##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431418_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4460269
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66276406198819	34.0	31.0	34.0	31.0	34.0
2	32.7790059299114	34.0	31.0	34.0	31.0	34.0
3	32.855832910526246	34.0	31.0	34.0	31.0	34.0
4	36.31930024848277	37.0	37.0	37.0	35.0	37.0
5	36.22165299895589	37.0	37.0	37.0	35.0	37.0
6	36.26992856260463	37.0	37.0	37.0	35.0	37.0
7	36.22735265518739	37.0	37.0	37.0	35.0	37.0
8	36.243029960749006	37.0	37.0	37.0	35.0	37.0
9	38.03907522169627	39.0	39.0	39.0	35.0	39.0
10-11	38.016140282121995	39.0	39.0	39.0	35.0	39.0
12-13	37.99994820940172	39.0	39.0	39.0	35.0	39.0
14-15	39.47316148420644	41.0	39.0	41.0	36.0	41.0
16-17	39.50827876076532	41.0	39.0	41.0	36.5	41.0
18-19	39.57692753508813	41.0	40.0	41.0	37.0	41.0
20-21	39.575582324743195	41.0	40.0	41.0	37.0	41.0
22-23	39.52172884639917	41.0	40.0	41.0	36.0	41.0
24-25	39.47558645005492	41.0	39.0	41.0	36.0	41.0
26-27	39.36383209174156	41.0	39.0	41.0	36.0	41.0
28-29	39.286145858018884	41.0	39.0	41.0	36.0	41.0
30-31	39.11854251391564	41.0	39.0	41.0	35.0	41.0
32-33	38.905120296556106	41.0	38.0	41.0	35.0	41.0
34-35	38.78024206611754	41.0	38.0	41.0	35.0	41.0
36-37	38.64411204795047	41.0	38.0	41.0	35.0	41.0
38-39	38.49863909553437	41.0	38.0	41.0	35.0	41.0
40-41	38.359108049312724	41.0	38.0	41.0	35.0	41.0
42-43	38.1891093339886	40.0	38.0	41.0	34.0	41.0
44-45	37.95838703898801	40.0	38.0	41.0	33.5	41.0
46-47	37.60462485558607	40.0	38.0	41.0	33.0	41.0
48-49	37.27535872836369	40.0	38.0	41.0	32.5	41.0
50-51	37.11143991539524	40.0	37.0	41.0	32.0	41.0
52-53	37.05104972368258	40.0	37.0	41.0	32.0	41.0
54-55	36.91295143857916	40.0	37.0	41.0	31.5	41.0
56-57	36.76331831107048	40.0	36.5	41.0	31.0	41.0
58-59	36.59086279325305	40.0	36.0	41.0	31.0	41.0
60-61	36.439944541461514	40.0	35.5	41.0	31.0	41.0
62-63	36.28512192874466	39.0	35.0	41.0	31.0	41.0
64-65	36.05634323849077	39.0	35.0	41.0	31.0	41.0
66-67	35.79725023311374	39.0	35.0	41.0	30.0	41.0
68-69	35.50561871940907	38.0	35.0	41.0	30.0	41.0
70-71	35.20625381563309	37.0	35.0	40.0	29.5	41.0
72-73	34.89021289971524	37.0	35.0	39.5	29.0	41.0
74-75	34.55171235187833	36.0	35.0	39.0	29.0	41.0
76-77	34.21620411235287	36.0	35.0	39.0	29.0	41.0
78-79	33.89250850116888	35.5	35.0	38.5	29.0	40.0
80-81	33.620874548149445	35.0	34.5	37.0	29.0	39.0
82-83	33.411150650330725	35.0	35.0	37.0	29.0	39.0
84-85	33.14428490747979	35.0	34.0	36.5	29.0	39.0
86-87	32.897681619651195	35.0	34.0	36.0	29.0	37.0
88-89	32.659875783276746	35.0	34.0	36.0	29.0	37.0
90-91	32.47495173497383	35.0	34.0	35.5	29.0	37.0
92-93	32.31245447303739	35.0	34.0	35.0	29.0	36.0
94-95	32.173003803133845	35.0	34.0	35.0	28.5	36.0
96-97	32.03150807720341	35.0	34.0	35.0	27.5	36.0
98-99	31.915710240794894	35.0	34.0	35.0	27.0	36.0
100	31.81380158909698	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.02875166406169427
1101	2	6.868000393467355E-4
1101	3	-0.006163464574704847
1101	4	0.035174611743919115
1101	5	0.006050968938197343
1101	6	0.04947426764092455
1101	7	0.012702224825687836
1101	8	0.029198548822300552
1101	9	-0.0077770302062987184
1101	10-11	0.02147314398759903
1101	12-13	0.027286315112029058
1101	14-15	0.005440110423975852
1101	16-17	0.031837019704205716
1101	18-19	0.01703001149412131
1101	20-21	0.0323316661516273
1101	22-23	0.026490896027574706
1101	24-25	0.02818823486055777
1101	26-27	0.026198748333776223
1101	28-29	-0.03982946952793043
1101	30-31	-0.056835468257816046
1101	32-33	-0.08988144967678124
1101	34-35	-0.065625938884871
1101	36-37	-0.1385603955330481
1101	38-39	-0.11178411224330631
1101	40-41	-0.1170963549266375
1101	42-43	-0.19432005862400814
1101	44-45	-0.1757080665123567
1101	46-47	-0.10775940349683566
1101	48-49	-0.07362198215402316
1101	50-51	-0.16915530745185947
1101	52-53	-0.15473464464781017
1101	54-55	-0.12721978161717118
1101	56-57	-0.14777810272960323
1101	58-59	-0.1313567890614351
1101	60-61	-0.1558824259394953
1101	62-63	-0.1635998630975024
1101	64-65	-0.17451488331239773
1101	66-67	-0.17024323505641803
1101	68-69	-0.17338712688757596
1101	70-71	-0.14669158573468621
1101	72-73	-0.12282631067395045
1101	74-75	-0.13853073722827958
1101	76-77	-0.16247699333974452
1101	78-79	-0.15482009769833383
1101	80-81	-0.10626243850342831
1101	82-83	-0.11626048833853275
1101	84-85	-0.08852101360790243
1101	86-87	-0.12028808309288763
1101	88-89	-0.12685118545672225
1101	90-91	-0.13088436581479357
1101	92-93	-0.14166934651463947
1101	94-95	-0.1445247881626699
1101	96-97	-0.09560265656750033
1101	98-99	-0.09252595780035477
1101	100	-0.02241159109424018
1102	1	-0.012731983838612848
1102	2	-0.004020868166890068
1102	3	0.01837197195391127
1102	4	0.015340117353538574
1102	5	0.02266997456940345
1102	6	-0.008159267859035424
1102	7	-0.026552191291401073
1102	8	0.009166529715635363
1102	9	0.03415237086782241
1102	10-11	0.016332563876076733
1102	12-13	-0.010245232540093241
1102	14-15	-0.008698676522854498
1102	16-17	-0.012038734150713992
1102	18-19	-0.014989901294718777
1102	20-21	-0.021272853846681983
1102	22-23	0.028818506022844304
1102	24-25	-0.013320812154354655
1102	26-27	0.0029739503941783596
1102	28-29	-0.005022735156885005
1102	30-31	-0.009734426241251981
1102	32-33	0.02846268407594721
1102	34-35	0.005759697629642346
1102	36-37	0.0323423924296975
1102	38-39	0.0032602427743810836
1102	40-41	0.01002318150973025
1102	42-43	0.07802090374986648
1102	44-45	0.16011394137361634
1102	46-47	0.05353350850216998
1102	48-49	0.09419880556779958
1102	50-51	0.04330949216245017
1102	52-53	0.025402090016967804
1102	54-55	0.11073117290256818
1102	56-57	0.08183625576801035
1102	58-59	0.05547695548572307
1102	60-61	-8.018438660712945E-4
1102	62-63	0.0298226570579061
1102	64-65	0.026094982121399823
1102	66-67	0.0192099099194607
1102	68-69	-0.020904658623159378
1102	70-71	-0.014658052028352131
1102	72-73	0.030659802817041282
1102	74-75	0.04137663096006605
1102	76-77	0.013129595969921581
1102	78-79	0.03976649826081058
1102	80-81	0.005823492028426358
1102	82-83	-0.023408719138295453
1102	84-85	0.005487211038129658
1102	86-87	-0.02363483850066217
1102	88-89	-0.01594541535191496
1102	90-91	-0.03586604697811424
1102	92-93	-0.050861609228910254
1102	94-95	-0.04624169385498078
1102	96-97	0.006607945035703722
1102	98-99	-0.014341186011307627
1102	100	-0.005838257228194976
1103	1	-0.10902730238446168
1103	2	-0.14845876151677118
1103	3	-0.11462706566981495
1103	4	-0.053024477093480016
1103	5	-0.07041121008236217
1103	6	-0.06380087211665852
1103	7	-0.07869922883496372
1103	8	-0.06447222054340074
1103	9	-0.0863045437425285
1103	10-11	-0.04589718785742747
1103	12-13	-0.02969178947518003
1103	14-15	-0.08079788208145544
1103	16-17	-0.09110834680113555
1103	18-19	-0.13339710034300367
1103	20-21	-0.11654813049050716
1103	22-23	-0.11268148875527118
1103	24-25	-0.09049263117444895
1103	26-27	-0.09591665846934916
1103	28-29	-0.0971097249978925
1103	30-31	-0.12321633650044816
1103	32-33	-0.09664277180652903
1103	34-35	-0.03208918875673561
1103	36-37	-0.08131909827262973
1103	38-39	-0.17799166639995434
1103	40-41	-0.13300496476087176
1103	42-43	-0.13586541292244902
1103	44-45	-0.16880045131118493
1103	46-47	-0.16933063104104917
1103	48-49	-0.18157830240927808
1103	50-51	-0.2703209870273753
1103	52-53	-0.16393762261334643
1103	54-55	-0.12199258882687758
1103	56-57	-0.12281870167910824
1103	58-59	-0.14232202665107252
1103	60-61	-0.20495044068007928
1103	62-63	-0.21402267454766388
1103	64-65	-0.17471739108231077
1103	66-67	-0.13480617339246947
1103	68-69	-0.12138982938861176
1103	70-71	-0.09957270515788252
1103	72-73	-0.11976102380716469
1103	74-75	-0.0835154521246011
1103	76-77	-0.09952909255291331
1103	78-79	-0.07638920551638506
1103	80-81	-0.05731806613167123
1103	82-83	-0.10188168747821891
1103	84-85	-0.03654356981186879
1103	86-87	-0.05665766444298015
1103	88-89	-0.0963435360122773
1103	90-91	-0.11761504981006965
1103	92-93	-0.11595812693987995
1103	94-95	-0.12146482458559404
1103	96-97	-0.06317574091991318
1103	98-99	-0.10135323877605273
1103	100	-0.06026503570420516
1104	1	-0.10998316990929169
1104	2	-0.09467816371220295
1104	3	-0.06913918475806469
1104	4	-0.03185603261697878
1104	5	-0.008094140191893473
1104	6	-0.04643104031561052
1104	7	-0.05030391249486854
1104	8	-0.07359855856000763
1104	9	-0.03070138781842502
1104	10-11	-0.04264856105184123
1104	12-13	-0.04308234356560092
1104	14-15	-0.11188730306182038
1104	16-17	-0.0654413232792237
1104	18-19	-0.09133068032296165
1104	20-21	-0.05006002913201968
1104	22-23	-0.040035163476673574
1104	24-25	-0.024872056367549078
1104	26-27	-0.04259221037423089
1104	28-29	-0.02337445523436088
1104	30-31	-0.0566405457233401
1104	32-33	-0.00854104204945827
1104	34-35	-0.02488513359872968
1104	36-37	-0.05529994020611184
1104	38-39	-0.1417676998799351
1104	40-41	-0.11826127922267204
1104	42-43	-0.09381995329196968
1104	44-45	-0.10498682189962949
1104	46-47	-0.12197182944522922
1104	48-49	-0.14390845010761666
1104	50-51	-0.2526454654628125
1104	52-53	-0.18714874003347148
1104	54-55	-0.15309871665147057
1104	56-57	-0.1664857824394801
1104	58-59	-0.12418134425356442
1104	60-61	-0.07997793505617778
1104	62-63	-0.10269672251693152
1104	64-65	-0.10705212720047541
1104	66-67	-0.1557231423681884
1104	68-69	-0.21167383688919728
1104	70-71	-0.15441140309701495
1104	72-73	-0.18089504259564393
1104	74-75	-0.12483051901320152
1104	76-77	-0.13877989914066546
1104	78-79	-0.13929626427239583
1104	80-81	-0.12250460720613887
1104	82-83	-0.12904266430269473
1104	84-85	-0.15041878465893177
1104	86-87	-0.18215654015472182
1104	88-89	-0.13257680869940458
1104	90-91	-0.16059423640140835
1104	92-93	-0.152906954134167
1104	94-95	-0.15629044422511384
1104	96-97	-0.11914759071105152
1104	98-99	-0.13955516534196022
1104	100	-0.020075892099253423
1105	1	-0.17376941028562243
1105	2	-0.1694686992915706
1105	3	-0.1763091918368076
1105	4	-0.0666449082843883
1105	5	-0.12364556938356941
1105	6	-0.02831786590735419
1105	7	-0.032590187557183015
1105	8	-0.032736994807684994
1105	9	-0.07193578072165963
1105	10-11	-0.12385015694965062
1105	12-13	-0.11960951742635473
1105	14-15	-0.19519996988038457
1105	16-17	-0.1919584675005268
1105	18-19	-0.23309926636370193
1105	20-21	-0.1275483436194591
1105	22-23	-0.17586059436476376
1105	24-25	-0.20896815630022303
1105	26-27	-0.13882870481184995
1105	28-29	-0.13376519547190213
1105	30-31	-0.17524546302203703
1105	32-33	-0.2485528823046792
1105	34-35	-0.19733289055829317
1105	36-37	-0.18663618211479616
1105	38-39	-0.09529995467719488
1105	40-41	-0.13281353979296995
1105	42-43	-0.164927806361014
1105	44-45	-0.18562114457247958
1105	46-47	-0.16063162270035036
1105	48-49	-0.2055999679116809
1105	50-51	-0.15134863874718008
1105	52-53	-0.22499181512985444
1105	54-55	-0.179593711030698
1105	56-57	-0.20011794011355732
1105	58-59	-0.19796886766178545
1105	60-61	-0.17190381849211178
1105	62-63	-0.22591485199627925
1105	64-65	-0.20605539877506374
1105	66-67	-0.20050349929218214
1105	68-69	-0.14278626074430179
1105	70-71	-0.14037494738906275
1105	72-73	-0.07694464743942575
1105	74-75	-0.14415725688905923
1105	76-77	-0.23457275775284359
1105	78-79	-0.18165403948786008
1105	80-81	-0.10375083313081745
1105	82-83	-0.18317272129838358
1105	84-85	-0.21250783498119574
1105	86-87	-0.23462802343870237
1105	88-89	-0.18860937460959093
1105	90-91	-0.1773734892572847
1105	92-93	-0.1840953833421608
1105	94-95	-0.1917585359019256
1105	96-97	-0.2164006486057346
1105	98-99	-0.19997568344523486
1105	100	-0.1831355255456515
1106	1	-0.15094818091777285
1106	2	-0.13018789930358565
1106	3	-0.13779019739017428
1106	4	-0.06558854230033262
1106	5	-0.1298085074387174
1106	6	-0.1577623963068504
1106	7	-0.13532162745386955
1106	8	-0.09989209720884418
1106	9	-0.1301139009909349
1106	10-11	-0.09352977708645227
1106	12-13	-0.10840022002084737
1106	14-15	-0.17553266832118197
1106	16-17	-0.21702533426493176
1106	18-19	-0.1597278204522965
1106	20-21	-0.15887061964533444
1106	22-23	-0.15272190313480394
1106	24-25	-0.11684136019382407
1106	26-27	-0.13083223794646415
1106	28-29	-0.12216732250146833
1106	30-31	-0.1815238204882661
1106	32-33	-0.17948490317225207
1106	34-35	-0.1596597281071368
1106	36-37	-0.1656358612142128
1106	38-39	-0.13882059339427144
1106	40-41	-0.18592449293601732
1106	42-43	-0.14401762909654536
1106	44-45	-0.14860273112947908
1106	46-47	-0.12047939914095451
1106	48-49	-0.12303240907527169
1106	50-51	-0.1667034907438918
1106	52-53	-0.13519146638264345
1106	54-55	-0.1322468860867687
1106	56-57	-0.15552741445182505
1106	58-59	-0.14723352156426017
1106	60-61	-0.14370022922446424
1106	62-63	-0.1548629993930959
1106	64-65	-0.11072770238791207
1106	66-67	-0.14062045139070278
1106	68-69	-0.15654727687677195
1106	70-71	-0.11383070514510507
1106	72-73	-0.07119311619063495
1106	74-75	-0.051535062611193894
1106	76-77	-0.0706152268561624
1106	78-79	-0.05079799565823606
1106	80-81	-0.08896759260944975
1106	82-83	-0.10931369036574523
1106	84-85	-0.04586722384500774
1106	86-87	-0.06796723612860234
1106	88-89	-0.1484361093476636
1106	90-91	-0.15137216738455095
1106	92-93	-0.1199400042993446
1106	94-95	-0.14920916065217682
1106	96-97	-0.1770958126540556
1106	98-99	-0.13561071781677825
1106	100	-0.06702345764853135
1107	1	-0.21595379429964368
1107	2	-0.1823006875837052
1107	3	-0.16851170447412755
1107	4	-0.21443447790693426
1107	5	-0.30759559908039336
1107	6	-0.2674556469549074
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2216	28-29	0.01008345659121801
2216	30-31	-0.04834607109515332
2216	32-33	-0.01578304310046974
2216	34-35	0.08389324089049666
2216	36-37	0.013948771856071573
2216	38-39	0.11454659985285076
2216	40-41	0.08428930561897374
2216	42-43	0.11144478501717003
2216	44-45	0.03870578613519626
2216	46-47	0.08287780215273699
2216	48-49	0.09838585127994293
2216	50-51	0.09651139778586781
2216	52-53	0.029839427691719322
2216	54-55	0.07636477384651386
2216	56-57	0.04960705117370878
2216	58-59	0.08321360939419975
2216	60-61	0.08042624170496282
2216	62-63	0.010711401375786522
2216	64-65	-0.0017181868528695077
2216	66-67	0.04043897280004671
2216	68-69	0.048488905291783624
2216	70-71	0.008014878482782706
2216	72-73	-0.028734662773196362
2216	74-75	-0.004506807757962861
2216	76-77	0.04603395602470073
2216	78-79	0.11375525525652819
2216	80-81	0.11018789721216393
2216	82-83	0.058568356251008424
2216	84-85	0.09009120794696912
2216	86-87	0.05823607127744879
2216	88-89	0.06482887422264838
2216	90-91	0.04466516939754683
2216	92-93	0.045565928007128775
2216	94-95	0.012031125207627724
2216	96-97	0.059240401259884834
2216	98-99	0.05575087467172679
2216	100	0.07924440881787476
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45.0
3	272.0
4	95.0
5	113.0
6	305.0
7	758.0
8	1384.0
9	2619.0
10	3718.0
11	5293.0
12	8020.0
13	11695.0
14	14050.0
15	18676.0
16	23780.0
17	36114.0
18	42623.0
19	21745.0
20	13201.0
21	11938.0
22	11439.0
23	11197.0
24	12271.0
25	14670.0
26	17793.0
27	22730.0
28	28634.0
29	36477.0
30	45927.0
31	58793.0
32	75349.0
33	97688.0
34	135719.0
35	218190.0
36	357702.0
37	749931.0
38	1406584.0
39	932976.0
40	9755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.12725680395999	21.131059483458905	15.057781221713928	22.68390249086718
2	14.225366354796956	21.91335195329062	40.814140704367105	23.047140987545315
3	16.370108516898355	28.117066687412994	32.11031635833781	23.402508437350836
4	11.143587976420257	18.839200057216278	39.83927875202146	30.177933214342005
5	11.419712934286032	38.89479789841628	35.701263605924346	13.98422556137335
6	27.33770093238771	40.15885588963356	18.556459262883024	13.946983915095704
7	23.95088726711326	35.21931076354363	23.632184516225365	17.19761745311774
8	24.451866019740066	35.5220279314992	21.260376896550408	18.765729152210326
9	25.408361111933186	16.128021903616556	22.4083171683854	36.05529981606486
10-11	22.541633699671475	27.65388141387885	29.75070561887635	20.053779267573326
12-13	24.296721150307434	26.000531133944065	29.758320603344846	19.94442711240366
14-15	21.588171027352836	27.13075153090542	28.520802220673236	22.760275221068504
16-17	19.29003609423557	30.34459356599344	29.11442112572134	21.250949214049648
18-19	19.18440944652392	28.0147480026411	33.11228144516077	19.688561105674214
20-21	20.785484014529167	27.099083037368377	32.892657371113714	19.22277557698874
22-23	20.295489036867075	27.14924913687903	33.0017463080125	19.553515518241397
24-25	20.006338182741892	27.244825368156043	32.99083530612167	19.75800114298039
26-27	19.8093813025256	27.143227236136504	33.051720800028605	19.995670661309287
28-29	19.693641486956658	27.236265921000875	33.155813965741274	19.9142786263012
30-31	19.997567407747862	27.544533533620495	32.80397734438276	19.65392171424888
32-33	19.744611080581954	27.359162121484225	33.067897817527715	19.82832898040611
34-35	19.96546593743736	27.22545498733923	32.842480910194396	19.96659816502901
36-37	20.191698677354907	27.463959810135307	32.36079358257166	19.983547929938126
38-39	20.12768167044188	27.535534578574705	32.38096529934647	19.955818451636944
40-41	20.09251692444283	27.413214237270655	32.396931572451415	20.0973372658351
42-43	20.100745473908415	27.494053023933635	32.16139229863797	20.243809203519984
44-45	20.34979319609781	27.661602541912316	31.764308121671718	20.224296140318152
46-47	20.51395589411035	27.91362667607717	31.193117181800368	20.379300248012115
48-49	20.571450158863826	27.93741136319993	30.71931912588631	20.771819352049928
50-51	20.571383859098766	28.09723605736797	30.507466046254265	20.823914037279
52-53	20.55768498659938	28.269889840058006	30.375078302926163	20.797346870416447
54-55	20.734751429270624	28.473994443559462	30.202750777734643	20.588503349435268
56-57	20.595113487151682	28.180063358507613	30.28130401624421	20.9435191380965
58-59	20.47506305700353	27.902382153466732	30.58829662014461	21.034258169385126
60-61	20.644854637541112	28.14779731090101	30.345867813991656	20.861480237566223
62-63	20.49440505247544	28.056269301837496	30.583437466614665	20.8658881790724
64-65	20.525621462327077	27.8943281991908	30.69268549479553	20.887364843686594
66-67	20.43422405221226	28.005730614056816	30.445019399115075	21.115025934615847
68-69	20.896019313667967	27.988193964433922	30.234519908139905	20.88126681375821
70-71	20.781810750536795	27.922110387710497	30.236100533130234	21.05997832862247
72-73	20.67530546965504	28.09548450394069	30.28531052989476	20.94389949650951
74-75	20.784990070618278	28.116152747128943	30.04842220078594	21.050434981466836
76-77	20.810691619578403	28.12908540143907	29.928562156170486	21.13166082281204
78-79	20.87450081116784	28.142165571507878	30.004948168661926	20.978385448662358
80-81	20.736031564195994	28.071303730390508	30.074322175459706	21.1183425299538
82-83	20.619083235580042	28.11154771257305	30.237143648906063	21.03222540294085
84-85	20.629547562574842	28.074271689349782	30.011138466519245	21.28504228155613
86-87	20.738086646221372	28.122293769924163	29.92538778297673	21.21423180087774
88-89	20.573175055835183	27.969258504979177	30.02940097983835	21.42816545934729
90-91	20.468402273786126	27.96618377633779	29.995632508307875	21.56978144156821
92-93	20.504086903278154	28.09431277315058	29.951921686426893	21.449678637144377
94-95	20.30879504241924	27.96064785751439	30.067642099976684	21.66291500008968
96-97	20.340695156317015	27.98146656374299	29.907429225586025	21.77040905435397
98-99	20.32842064765124	27.961828644121393	29.923296994981563	21.7864537132458
100	20.377695131947345	27.936709575935414	29.84455628635435	21.841039005762887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3698.0
1	2109.5
2	1775.5
3	3110.5
4	2000.5
5	1240.5
6	3143.0
7	8261.0
8	14938.0
9	16698.0
10	10826.5
11	5362.5
12	4497.5
13	4812.5
14	5396.0
15	5965.5
16	6611.0
17	7372.0
18	8336.5
19	9539.0
20	11279.5
21	13822.5
22	17750.0
23	23483.0
24	30463.5
25	38574.0
26	48872.5
27	61294.0
28	76318.5
29	91215.5
30	102781.5
31	113563.0
32	123136.0
33	132528.5
34	141262.0
35	147184.0
36	150202.5
37	152647.5
38	153833.0
39	152265.5
40	151408.0
41	149966.5
42	145512.0
43	141141.5
44	137342.0
45	132313.0
46	129451.0
47	126987.0
48	122759.0
49	119570.5
50	116989.0
51	111614.0
52	104825.5
53	100045.5
54	96742.5
55	92549.0
56	84792.5
57	75920.5
58	69446.5
59	64142.5
60	56471.5
61	47105.0
62	39766.0
63	32676.5
64	25633.5
65	20197.5
66	15595.0
67	11558.5
68	8458.0
69	6301.5
70	4518.5
71	3333.0
72	2579.5
73	1895.5
74	1443.0
75	1055.0
76	646.0
77	456.5
78	352.5
79	185.5
80	83.0
81	41.0
82	23.5
83	14.5
84	14.0
85	9.0
86	5.0
87	2.5
88	1.5
89	1.0
90	0.0
91	0.5
92	0.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005739564138396137
2	0.004035631034809784
3	9.416472414556163E-4
4	0.0
5	0.0429794705207242
6	0.0
7	0.0
8	0.0
9	2.242017241560991E-5
10-11	0.0
12-13	1.1210086207804956E-5
14-15	0.0
16-17	0.0
18-19	8.968068966243965E-5
20-21	0.0
22-23	4.484034483121982E-5
24-25	0.0
26-27	7.847060345463469E-5
28-29	1.0089077587024459E-4
30-31	1.457311207014644E-4
32-33	0.0011658489656117153
34-35	7.847060345463468E-4
36-37	8.968068966243965E-4
38-39	8.071262069619567E-4
40-41	8.968068966243965E-5
42-43	4.2598327589658835E-4
44-45	1.1210086207804956E-4
46-47	6.726051724682973E-5
48-49	5.605043103902478E-5
50-51	2.2420172415609912E-4
52-53	6.053446552214676E-4
54-55	8.855968104165915E-4
56-57	0.0021187062932751365
58-59	4.2598327589658835E-4
60-61	8.743867242087865E-4
62-63	8.855968104165915E-4
64-65	0.0014124708621834244
66-67	3.1388241381853876E-4
68-69	1.793613793248793E-4
70-71	1.793613793248793E-4
72-73	1.3452103449365947E-4
74-75	4.8203370693561306E-4
76-77	6.277648276370775E-4
78-79	7.398656897151271E-4
80-81	5.717143965980527E-4
82-83	0.0013676305173522046
84-85	0.0011210086207804955
86-87	0.0016703028449629384
88-89	9.752775000790311E-4
90-91	9.640674138712261E-4
92-93	0.001031327931118056
94-95	6.501850000526874E-4
96-97	1.3452103449365947E-4
98-99	3.026723276107338E-4
100	1.1210086207804956E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4460269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.094818901043645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61983366216013	44.2417057145247
2	16.33410324503667	19.63189952842856
3	5.042620857333782	9.091061616242973
4	2.0205568219355645	4.856999851739443
5	0.9789563816945784	2.941510323497833
6	0.5321398985578472	1.9187310500312138
7	0.34476556201915487	1.4503036809000351
8	0.21367531578059848	1.0272623524366704
9	0.1451921291638927	0.7852765237164964
>10	0.7041552811594929	7.32489182077716
>50	0.04045666477914781	1.6777195887523018
>100	0.02193530763347137	2.2827285954541274
>500	8.231445696555784E-4	0.3173964382458069
>1k	6.360656662356932E-4	0.7745582348188804
>5k	3.741562742561807E-5	0.11683129900482644
>10k+	1.1224688227685423E-4	1.561123381429003
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29520	0.6618434897088046	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24454	0.5482628962513247	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15456	0.3465261848556668	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5196	0.11649521587150909	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4821	0.10808765121565539	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4621	0.10360361673253339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.242017241560991E-5	0.0
5	0.0	0.0	0.0	2.242017241560991E-5	0.0
6	0.0	0.0	0.0	2.242017241560991E-5	0.0
7	0.0	0.0	0.0	4.484034483121982E-5	0.0
8	0.0	0.0	0.0	4.484034483121982E-5	0.0
9	0.0	0.0	0.0	4.484034483121982E-5	0.0
10-11	0.0	0.0	0.0	4.484034483121982E-5	0.0
12-13	0.0	0.0	0.0	4.484034483121982E-5	0.0
14-15	0.0	0.0	0.0	5.605043103902478E-5	0.0
16-17	0.0	0.0	0.0	6.726051724682973E-5	0.0
18-19	0.0	2.242017241560991E-5	0.0	6.726051724682973E-5	0.0
20-21	0.0	2.242017241560991E-5	0.0	1.008907758702446E-4	0.0
22-23	0.0	4.484034483121982E-5	0.0	2.3541181036390406E-4	0.0
24-25	0.0	4.484034483121982E-5	0.0	6.389749138448824E-4	0.0
26-27	0.0	4.484034483121982E-5	0.0	0.0010537481035336658	0.0
28-29	0.0	4.484034483121982E-5	0.0	0.002040235689820502	0.0
30-31	0.0	6.726051724682973E-5	0.0	0.005123009396966864	0.0
32-33	0.0	6.726051724682973E-5	0.0	0.011187666035389347	0.0
34-35	0.0	6.726051724682973E-5	0.0	0.01993153327747721	0.0
36-37	0.0	6.726051724682973E-5	0.0	0.035166040433884146	0.0
38-39	0.0	6.726051724682973E-5	0.0	0.056341893280427706	0.0
40-41	0.0	6.726051724682973E-5	0.0	0.07866117492016737	0.0
42-43	0.0	6.726051724682973E-5	0.0	0.10126070871510215	0.0
44-45	0.0	7.847060345463469E-5	0.0	0.12900567207941943	0.0
46-47	0.0	8.968068966243965E-5	0.0	0.1635327375994587	0.0
48-49	0.0	8.968068966243965E-5	0.0	0.20387783786134872	0.0
50-51	0.0	1.3452103449365947E-4	0.0	0.24427898855427777	0.0
52-53	0.0	1.793613793248793E-4	0.0	0.2846577190747912	0.0
54-55	0.0	2.2420172415609912E-4	0.0	0.3303948708026354	0.0
56-57	0.0	2.6904206898731894E-4	0.0	0.3877568819279734	0.0
58-59	0.0	2.6904206898731894E-4	0.0	0.452551180209086	0.0
60-61	0.0	2.6904206898731894E-4	0.0	0.5238809587493489	0.0
62-63	0.0	2.914622414029288E-4	0.0	0.5959281828069114	0.0
64-65	0.0	2.914622414029288E-4	0.0	0.6742082147960133	0.0
66-67	0.0	2.914622414029288E-4	0.0	0.7671622496311321	0.0
68-69	0.0	2.914622414029288E-4	0.0	0.8739159005880587	0.0
70-71	0.0	2.914622414029288E-4	0.0	0.9951978232703005	0.0
72-73	0.0	2.914622414029288E-4	0.0	1.1223202008668087	0.0
74-75	0.0	3.1388241381853876E-4	0.0	1.2500815533771616	0.0
76-77	0.0	3.1388241381853876E-4	0.0	1.3938621190784681	0.0
78-79	0.0	3.1388241381853876E-4	0.0	1.5623945551266079	0.0
80-81	0.0	3.1388241381853876E-4	0.0	1.7529436004868764	0.0
82-83	0.0	3.1388241381853876E-4	0.0	1.9592652371415267	0.0
84-85	0.0	3.250925000263437E-4	0.0	2.1686696475033234	0.0
86-87	0.0	3.3630258623414865E-4	0.0	2.3929274220904615	0.0
88	0.0	3.3630258623414865E-4	0.0	2.5794184162435045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11820	0.0	64.1661	1
GTATCAA	23290	0.0	44.61815	1
GTGGTAT	3715	0.0	37.08494	1
ATCAACG	29975	0.0	34.240067	3
TATCAAC	30265	0.0	34.13214	2
TCAACGC	30300	0.0	33.918976	4
TGGTATC	3940	0.0	33.29524	2
CAACGCA	31220	0.0	32.94956	5
AACGCAG	32350	0.0	31.903433	6
ACGCAGA	36190	0.0	28.388401	7
CGCAGAG	36190	0.0	28.232563	8
TATAACG	380	0.0	27.221573	2
GCAGAGT	38945	0.0	26.090555	9
GTACATG	20145	0.0	26.07202	1
CATGGGT	9660	0.0	25.989586	4
TACATGG	19975	0.0	25.657473	2
ACATGGG	20130	0.0	24.593744	3
GAGTACT	27475	0.0	23.298008	12-13
CAGAGTA	38570	0.0	22.512005	10-11
AGAGTAC	37275	0.0	22.146742	10-11
>>END_MODULE