FastQCFastQC Report
Wed 25 May 2016
SRR2431417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4470602
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311260.6962373300061154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68500.049.0479131
GTATCAA115050.035.7059441
TCAACGC132300.029.8696484
ATCAACG133500.029.5688973
TATCAAC144050.028.0568732
CAACGCA141650.028.0302375
GTACATG235700.027.071541
TACATGG235750.026.3439922
AACGCAG152300.025.9827466
ACATGGG242600.024.53323
TACCTGG36200.024.0448722
CATGGGT100650.024.0248134
GTGGTAT32750.022.9890791
CGCAGAG171750.022.4104358
ACGCAGA176250.022.238977
ATGGGTG61000.020.8225965
GTACCTG43600.020.7218071
TGGTATC33600.020.4443322
GCAGAGT186900.019.839739
CATGGGG112150.019.589724
Produced by FastQC (version 0.11.5)