##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431417_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4470602 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.595369930045216 31.0 31.0 34.0 26.0 34.0 2 30.709680485983768 33.0 31.0 34.0 26.0 34.0 3 30.793419543945088 33.0 31.0 34.0 26.0 34.0 4 34.01937636139383 37.0 35.0 37.0 30.0 37.0 5 34.14759220346611 37.0 35.0 37.0 30.0 37.0 6 34.2688772116149 37.0 35.0 37.0 30.0 37.0 7 34.23060496103209 37.0 35.0 37.0 30.0 37.0 8 34.19161133109143 37.0 35.0 37.0 30.0 37.0 9 35.64765483485222 39.0 37.0 39.0 30.0 39.0 10-11 35.66484256482684 39.0 37.0 39.0 30.0 39.0 12-13 35.59932610865383 39.0 37.0 39.0 30.0 39.0 14-15 36.949979779009624 41.0 38.0 41.0 30.0 41.0 16-17 36.973543495931864 41.0 38.0 41.0 30.0 41.0 18-19 36.9984256035317 41.0 38.0 41.0 30.0 41.0 20-21 37.082907961836014 41.0 38.0 41.0 30.0 41.0 22-23 37.06476711637493 40.5 38.0 41.0 30.0 41.0 24-25 37.114991336737205 41.0 38.0 41.0 30.0 41.0 26-27 37.15507665410609 40.5 38.0 41.0 30.0 41.0 28-29 37.12605729608674 40.0 38.0 41.0 30.0 41.0 30-31 37.0579316834735 40.0 38.0 41.0 30.0 41.0 32-33 37.00518218799168 40.0 38.0 41.0 30.0 41.0 34-35 36.93385141419433 40.0 38.0 41.0 30.0 41.0 36-37 36.80431841170384 40.0 37.5 41.0 30.0 41.0 38-39 36.687313475903245 40.0 37.0 41.0 29.5 41.0 40-41 36.62028089281935 40.0 37.0 41.0 29.0 41.0 42-43 36.461287875771546 40.0 37.0 41.0 29.0 41.0 44-45 36.31338251537488 40.0 36.0 41.0 28.0 41.0 46-47 36.17917229044321 40.0 36.0 41.0 28.0 41.0 48-49 36.045453721892486 40.0 36.0 41.0 28.0 41.0 50-51 35.91097429384231 40.0 35.0 41.0 28.0 41.0 52-53 35.750778754181205 40.0 35.0 41.0 28.0 41.0 54-55 35.5912429690677 39.5 35.0 41.0 27.5 41.0 56-57 35.403804342234 39.0 35.0 41.0 27.5 41.0 58-59 35.193511522609256 39.0 35.0 41.0 27.0 41.0 60-61 34.95888842263301 39.0 34.0 41.0 26.5 41.0 62-63 34.85753093207582 38.5 34.0 41.0 26.5 41.0 64-65 34.61457069987442 38.0 34.0 40.0 26.0 41.0 66-67 34.34992278444827 37.5 34.0 40.0 26.0 41.0 68-69 34.056278326721994 37.0 34.0 40.0 26.0 41.0 70-71 33.72692514341469 36.5 33.5 39.5 25.5 41.0 72-73 33.38186221900317 36.0 33.0 39.0 25.0 41.0 74-75 33.092031341640336 35.5 33.0 39.0 24.5 41.0 76-77 32.82122452412449 35.0 33.0 38.5 24.0 40.0 78-79 32.46968461965525 35.0 33.0 37.0 23.5 39.5 80-81 32.142212502924664 35.0 33.0 37.0 23.0 39.0 82-83 31.81384889551787 35.0 32.5 36.5 21.5 39.0 84-85 31.512343192259117 35.0 32.0 36.0 20.5 37.5 86-87 31.230401968236045 35.0 32.0 36.0 20.0 37.0 88-89 30.967664310086203 35.0 32.0 35.0 18.5 37.0 90-91 30.739999221581343 35.0 32.0 35.0 17.5 36.0 92-93 30.51082460482951 35.0 32.0 35.0 10.0 36.0 94-95 30.303731018775547 35.0 31.0 35.0 4.5 36.0 96-97 30.10638679086172 35.0 31.0 35.0 2.0 36.0 98-99 29.900712141228407 35.0 31.0 35.0 2.0 35.0 100 29.734194634190207 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.163211022878464 1101 2 0.21073341843391802 1101 3 0.2043655825402837 1101 4 0.2540092294841756 1101 5 0.18753529517606893 1101 6 0.24377813766724188 1101 7 0.19777453563443714 1101 8 0.15480441939439515 1101 9 0.19794737413605645 1101 10-11 0.2524217249422378 1101 12-13 0.19710174004409708 1101 14-15 0.2516653234411521 1101 16-17 0.16974953734973752 1101 18-19 0.12462711539407678 1101 20-21 0.14383824556337288 1101 22-23 0.23306520281528975 1101 24-25 0.2326274571673892 1101 26-27 0.2002596081254424 1101 28-29 0.18065092656200932 1101 30-31 0.22092782379197473 1101 32-33 0.1837478972672173 1101 34-35 0.16057921337623782 1101 36-37 0.20152784290932146 1101 38-39 0.22380168203739714 1101 40-41 0.12476199329601911 1101 42-43 0.25589945841479533 1101 44-45 0.1920379077025629 1101 46-47 0.16448377060933694 1101 48-49 0.2315966693682867 1101 50-51 0.22533828847713266 1101 52-53 0.14376062370745757 1101 54-55 0.17758020447077172 1101 56-57 0.13196404249021754 1101 58-59 0.13841735838695257 1101 60-61 0.20868250756511486 1101 62-63 0.14722442896314902 1101 64-65 0.163878324495478 1101 66-67 0.23214315194397983 1101 68-69 0.2363700657339507 1101 70-71 0.2721960250699311 1101 72-73 0.19518308774645732 1101 74-75 0.2008051106377735 1101 76-77 0.2585359943708667 1101 78-79 0.19064090325402105 1101 80-81 0.13293792084297706 1101 82-83 0.16289320770107807 1101 84-85 0.16379286462599651 1101 86-87 0.1671350726220311 1101 88-89 0.1173930776314478 1101 90-91 0.12702105772046224 1101 92-93 0.17381601248809275 1101 94-95 0.13306954815779193 1101 96-97 0.09519896247935833 1101 98-99 0.1530404897358828 1101 100 0.11426715604692816 1102 1 -0.0134914190231612 1102 2 0.1146853526165792 1102 3 0.13988714510897182 1102 4 0.16969958175673838 1102 5 0.1650973460973617 1102 6 0.14275851436740794 1102 7 0.14246464205100295 1102 8 0.17802719513908016 1102 9 0.12864006994012556 1102 10-11 0.2048447646702911 1102 12-13 0.13546964705010822 1102 14-15 0.16520043054095623 1102 16-17 0.1106961051085591 1102 18-19 0.11568898878350353 1102 20-21 0.08504792358625934 1102 22-23 0.11021769700635531 1102 24-25 0.14716224468211436 1102 26-27 0.10914181427694558 1102 28-29 0.04066104906593182 1102 30-31 0.08448970618965745 1102 32-33 0.0797816607515287 1102 34-35 0.0688275157364302 1102 36-37 0.0725180410991797 1102 38-39 0.05861229946855673 1102 40-41 0.041668251758572694 1102 42-43 0.027354759605017875 1102 44-45 -0.058333412460982004 1102 46-47 -0.09782259700551776 1102 48-49 -0.07926093566169357 1102 50-51 -0.11292585732017812 1102 52-53 -0.13756094512542916 1102 54-55 -0.09497147032965358 1102 56-57 -0.15901762973523148 1102 58-59 -0.11132438194643157 1102 60-61 -0.17018529646963998 1102 62-63 -0.06232649441043492 1102 64-65 -0.11182058423096208 1102 66-67 -0.08100988052481028 1102 68-69 -0.06554729228143685 1102 70-71 -0.08387002121917675 1102 72-73 -0.13113245664440853 1102 74-75 -0.07704434511133229 1102 76-77 -0.028892809252837992 1102 78-79 -0.009894450256126675 1102 80-81 -0.017652313712517298 1102 82-83 -0.008213626568977617 1102 84-85 -0.08087883723798939 1102 86-87 -0.06544087456799019 1102 88-89 -0.06016787844071203 1102 90-91 -0.10168480565400628 1102 92-93 -0.1320691619518648 1102 94-95 -0.07365105160887708 1102 96-97 -0.05065329164871102 1102 98-99 -0.1330849444965274 1102 100 -0.13178685521699052 1103 1 -0.21968941922613539 1103 2 -0.29321877446250255 1103 3 -0.25919528674462455 1103 4 -0.09254371264442796 1103 5 -0.06409053316992441 1103 6 0.04210916880111171 1103 7 -0.05834627428252759 1103 8 -0.08607057186090117 1103 9 -0.10967608388183692 1103 10-11 -0.05207133622402438 1103 12-13 -0.02728266736701812 1103 14-15 -0.042053169919050504 1103 16-17 -0.0439148966178351 1103 18-19 -0.08611233644010952 1103 20-21 -0.09415476690691094 1103 22-23 -0.05351007087667625 1103 24-25 0.0031520568658791603 1103 26-27 -0.0349916836896611 1103 28-29 -0.03795093650489889 1103 30-31 -0.10403764321615228 1103 32-33 -0.07172230696782833 1103 34-35 -0.07950559992612938 1103 36-37 -0.15146894864309246 1103 38-39 -0.08642673638586729 1103 40-41 -0.07256116829369574 1103 42-43 -0.13868397151032497 1103 44-45 -0.29389197283454394 1103 46-47 -0.2653034897001447 1103 48-49 -0.32752943675053103 1103 50-51 -0.3579385529509338 1103 52-53 -0.42448726761958255 1103 54-55 -0.4073680063293068 1103 56-57 -0.3450124311803435 1103 58-59 -0.29572510546486086 1103 60-61 -0.3450829259731165 1103 62-63 -0.2337346897356909 1103 64-65 -0.26490202647219263 1103 66-67 -0.2342289840810281 1103 68-69 -0.2837325250291798 1103 70-71 -0.20336524749995988 1103 72-73 -0.21634743365714115 1103 74-75 -0.27021913721514323 1103 76-77 -0.25596333554506856 1103 78-79 -0.18880191875326346 1103 80-81 -0.21096445126836016 1103 82-83 -0.20805278365984492 1103 84-85 -0.2448154866654093 1103 86-87 -0.25254605789278983 1103 88-89 -0.32122632970422416 1103 90-91 -0.3472182219631641 1103 92-93 -0.31554734591566813 1103 94-95 -0.38647098129778357 1103 96-97 -0.4018965625661508 1103 98-99 -0.4927231660562619 1103 100 -0.45715146063069056 1104 1 -0.4424232500011449 1104 2 -0.4024859230806257 1104 3 -0.37145559263749206 1104 4 -0.29288171493233506 1104 5 -0.2836389436100859 1104 6 -0.244640498154709 1104 7 -0.2437914129807055 1104 8 -0.24264833724414103 1104 9 -0.29772371258869157 1104 10-11 -0.3306836658903052 1104 12-13 -0.3166801917142692 1104 14-15 -0.41268579833213437 1104 16-17 -0.3302488797783312 1104 18-19 -0.3753182889384874 1104 20-21 -0.42579795144462196 1104 22-23 -0.33919059358123604 1104 24-25 -0.3798788139565801 1104 26-27 -0.36683436242025635 1104 28-29 -0.3550179119480248 1104 30-31 -0.4041631645157011 1104 32-33 -0.4166462378435156 1104 34-35 -0.39570897340908573 1104 36-37 -0.5081602454696679 1104 38-39 -0.43480342682931195 1104 40-41 -0.466628980899948 1104 42-43 -0.5515673777354237 1104 44-45 -0.5679709671115845 1104 46-47 -0.6415690243400647 1104 48-49 -0.6086503713319047 1104 50-51 -0.6370762297315267 1104 52-53 -0.7137510425952343 1104 54-55 -0.6595456274089173 1104 56-57 -0.6046533655596775 1104 58-59 -0.667225150792369 1104 60-61 -0.6628465359885496 1104 62-63 -0.6339437412529918 1104 64-65 -0.6347017320693809 1104 66-67 -0.6042997109401327 1104 68-69 -0.5343213971133594 1104 70-71 -0.5894573731951311 1104 72-73 -0.547999643818315 1104 74-75 -0.46813545439921 1104 76-77 -0.5301602610907139 1104 78-79 -0.5391297788457337 1104 80-81 -0.5703907246854669 1104 82-83 -0.5704616866730738 1104 84-85 -0.5766772268747324 1104 86-87 -0.5340222750426449 1104 88-89 -0.5598885249432151 1104 90-91 -0.5401395544163528 1104 92-93 -0.6142152489642534 1104 94-95 -0.636815696310066 1104 96-97 -0.6716088033586196 1104 98-99 -0.700743808281878 1104 100 -0.631115713844757 1105 1 -0.2113880305017517 1105 2 -0.20071926667225526 1105 3 -0.08810051028629218 1105 4 -0.012385557088364862 1105 5 -0.028999468482979296 1105 6 0.05390356030304844 1105 7 -0.013577777589162565 1105 8 -0.047186236838236084 1105 9 -0.05062955626802079 1105 10-11 -0.08010897634692782 1105 12-13 -0.03640996182924994 1105 14-15 -0.03177379087988896 1105 16-17 -0.03503649164412792 1105 18-19 0.0467319116607996 1105 20-21 0.020402914977339037 1105 22-23 -0.00877343381580431 1105 24-25 -0.06846650136350974 1105 26-27 -0.018746905492569965 1105 28-29 -0.05357422308549786 1105 30-31 -0.03731253889775843 1105 32-33 0.0010162147399981336 1105 34-35 -0.036093105145639015 1105 36-37 -0.013868633942614395 1105 38-39 -0.031396731854663074 1105 40-41 -0.11063123281565623 1105 42-43 -0.12126213873299463 1105 44-45 -0.13604431913894643 1105 46-47 -0.15932265097472964 1105 48-49 -0.21682777704278067 1105 50-51 -0.1308417328743161 1105 52-53 -0.15679395469105373 1105 54-55 -0.1275532210020458 1105 56-57 -0.18551496622397678 1105 58-59 -0.17957680822298272 1105 60-61 -0.3128694253852018 1105 62-63 -0.1856779965851345 1105 64-65 -0.13523402815917507 1105 66-67 -0.11493297606552488 1105 68-69 -0.05167011257125864 1105 70-71 -0.15160704550753934 1105 72-73 -0.08043480356799648 1105 74-75 -0.1472791690251043 1105 76-77 -0.10895319929251457 1105 78-79 -0.16634647321388485 1105 80-81 -0.14011312580567648 1105 82-83 -0.13586578526603077 1105 84-85 -0.17600345222196978 1105 86-87 -0.16189601680340715 1105 88-89 -0.0947760104673101 1105 90-91 -0.160331029640016 1105 92-93 -0.22187979104898758 1105 94-95 -0.17873433299387642 1105 96-97 -0.15603296781126375 1105 98-99 -0.2280172198748538 1105 100 -0.11632004057483769 1106 1 -0.3903957500987616 1106 2 -0.41726033710770594 1106 3 -0.3484067892596343 1106 4 -0.2846204565441255 1106 5 -0.2591432072584112 1106 6 -0.2202099706586722 1106 7 -0.21074256401611535 1106 8 -0.3507568404194572 1106 9 -0.22386819242121447 1106 10-11 -0.29989767818326385 1106 12-13 -0.25409519970086336 1106 14-15 -0.26335402558479615 1106 16-17 -0.2788218612719007 1106 18-19 -0.3472651518474663 1106 20-21 -0.36308038626004446 1106 22-23 -0.2517363264940826 1106 24-25 -0.2771244979330518 1106 26-27 -0.33689031716809126 1106 28-29 -0.3377572120964629 1106 30-31 -0.27416757651912604 1106 32-33 -0.33560322517060115 1106 34-35 -0.292760641219715 1106 36-37 -0.31642745490920987 1106 38-39 -0.2971029616265639 1106 40-41 -0.2736105204512995 1106 42-43 -0.2935779798232616 1106 44-45 -0.33446662735322263 1106 46-47 -0.32329364298222174 1106 48-49 -0.2957115865910964 1106 50-51 -0.3614091371922328 1106 52-53 -0.3633813093747733 1106 54-55 -0.3212213473374703 1106 56-57 -0.32652741356488235 1106 58-59 -0.3692888871963689 1106 60-61 -0.3906232489746486 1106 62-63 -0.3657875097540213 1106 64-65 -0.35437978762455913 1106 66-67 -0.3158283194921694 1106 68-69 -0.3577749363983287 1106 70-71 -0.3577251931005563 1106 72-73 -0.42116026849140553 1106 74-75 -0.34963213439636576 1106 76-77 -0.3240067253535912 1106 78-79 -0.3003540017751831 1106 80-81 -0.3276906975118088 1106 82-83 -0.3516305782037925 1106 84-85 -0.31982982629990175 1106 86-87 -0.3199599605484593 1106 88-89 -0.36083343892005004 1106 90-91 -0.42798143760846585 1106 92-93 -0.4128379150471453 1106 94-95 -0.4605736013972894 1106 96-97 -0.44630076088684234 1106 98-99 -0.5039471982702786 1106 100 -0.5002734017409018 1107 1 -0.4165779009278694 1107 2 -0.3470449138049503 1107 3 -0.39933182253079025 1107 4 -0.2642643623674914 1107 5 -0.3002895556679448 1107 6 -0.3274872735022285 1107 7 -0.32071345917744054 1107 8 -0.2211546919915648 1107 9 -0.29056415805933966 1107 10-11 -0.287027169647736 1107 12-13 -0.2554334907707414 1107 14-15 -0.2776429285216082 1107 16-17 -0.27371982093638536 1107 18-19 -0.3385381294880858 1107 20-21 -0.2870402963332168 1107 22-23 -0.31503065704416144 1107 24-25 -0.32638364106866646 1107 26-27 -0.3399598768278267 1107 28-29 -0.3785458991312396 1107 30-31 -0.38348873942347694 1107 32-33 -0.392642545728485 1107 34-35 -0.3433712453552715 1107 36-37 -0.31563888473106516 1107 38-39 -0.4123197061630961 1107 40-41 -0.3843586535253607 1107 42-43 -0.4583893355353936 1107 44-45 -0.45638795004958865 1107 46-47 -0.4564461830965598 1107 48-49 -0.3921484584749635 1107 50-51 -0.49029386604811265 1107 52-53 -0.47988748194901376 1107 54-55 -0.4405499843538365 1107 56-57 -0.4580139457446606 1107 58-59 -0.5484506133722746 1107 60-61 -0.5918533722753452 1107 62-63 -0.5474724908694313 1107 64-65 -0.5212309511113133 1107 66-67 -0.43858408330382304 1107 68-69 -0.49291173027096846 1107 70-71 -0.40529528681391014 1107 72-73 -0.40469703130280266 1107 74-75 -0.36142810386059665 1107 76-77 -0.3496850733477146 1107 78-79 -0.2856343785134072 1107 80-81 -0.36948230181567254 1107 82-83 -0.3416624725231827 1107 84-85 -0.3290602509776086 1107 86-87 -0.34546959679074973 1107 88-89 -0.4205556085253761 1107 90-91 -0.41447137465101846 1107 92-93 -0.4148050172686091 1107 94-95 -0.42719449607799476 1107 96-97 -0.4366130639857886 1107 98-99 -0.37508798468143567 1107 100 -0.3615539863708257 1108 1 -0.33083561747458035 1108 2 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2215 12-13 -0.19147812832212452 2215 14-15 -0.2045012786991478 2215 16-17 -0.10735389890068348 2215 18-19 -0.0622573843887011 2215 20-21 -0.1153334132763888 2215 22-23 -0.11392860757965906 2215 24-25 -0.023093124968760037 2215 26-27 -0.06777585477485815 2215 28-29 -0.06430828391115995 2215 30-31 -0.10506255734701853 2215 32-33 -0.1332158369472225 2215 34-35 -0.15144736754425026 2215 36-37 -0.17956627790176327 2215 38-39 -0.16035483034723086 2215 40-41 -0.136194385898321 2215 42-43 -0.09612509312699302 2215 44-45 -0.07673566781643615 2215 46-47 -0.022327041053486596 2215 48-49 -0.023940431319431354 2215 50-51 -0.017194238227418168 2215 52-53 0.06507796632470786 2215 54-55 0.0434426559405523 2215 56-57 0.07720386875175933 2215 58-59 0.04052663156398495 2215 60-61 0.13303751486003534 2215 62-63 0.06671626662467389 2215 64-65 0.05114106697421761 2215 66-67 0.09289570664608249 2215 68-69 0.10314849599967602 2215 70-71 0.14201753825744845 2215 72-73 0.13397761709314437 2215 74-75 0.1413064853940753 2215 76-77 0.18819831954629507 2215 78-79 0.252552863683114 2215 80-81 0.250748772896344 2215 82-83 0.2202861555115625 2215 84-85 0.24515784838604304 2215 86-87 0.18433977092927023 2215 88-89 0.226124380994996 2215 90-91 0.2761601972441916 2215 92-93 0.24868516189272682 2215 94-95 0.29889136279670936 2215 96-97 0.3313722438369524 2215 98-99 0.3173598745385924 2215 100 0.3595607214387293 2216 1 0.3384009549184732 2216 2 0.23292850455077385 2216 3 0.2599057900522048 2216 4 0.09757156263390954 2216 5 0.2462402056011257 2216 6 0.11138202211061099 2216 7 -0.009248397972790201 2216 8 0.08685065687244276 2216 9 -0.016942552688163914 2216 10-11 0.02272543852557618 2216 12-13 -0.01162898184189487 2216 14-15 0.10967890321185081 2216 16-17 0.11268930990701165 2216 18-19 0.11118280233143452 2216 20-21 0.08001263551207671 2216 22-23 0.0936069301656346 2216 24-25 0.058434304139808546 2216 26-27 0.05620230056842246 2216 28-29 0.07490767589604275 2216 30-31 0.018323225760106254 2216 32-33 0.03416616329874245 2216 34-35 0.03874966225302501 2216 36-37 0.040041742791792956 2216 38-39 0.10899269544579226 2216 40-41 0.07878099633932578 2216 42-43 0.1515636965878997 2216 44-45 0.19541561726115475 2216 46-47 0.236859011552923 2216 48-49 0.22849757456533837 2216 50-51 0.27624624625944705 2216 52-53 0.22903934067724663 2216 54-55 0.2494134907091734 2216 56-57 0.25449375098502003 2216 58-59 0.30187555155793433 2216 60-61 0.36140483337070606 2216 62-63 0.32659366831219216 2216 64-65 0.2610513963592851 2216 66-67 0.19009810664592663 2216 68-69 0.22226020481970465 2216 70-71 0.12812700587296888 2216 72-73 0.161533215342196 2216 74-75 0.13305636868378912 2216 76-77 0.13276883896771352 2216 78-79 0.1713053964848683 2216 80-81 0.16775555647112128 2216 82-83 0.1569802146778123 2216 84-85 0.18963061466138953 2216 86-87 0.14680399048369352 2216 88-89 0.18927821605710804 2216 90-91 0.20862929323867618 2216 92-93 0.2026165495040395 2216 94-95 0.19680836215650999 2216 96-97 0.19978484328542834 2216 98-99 0.19449251146489033 2216 100 0.1232883285139117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 105167.0 3 20666.0 4 15150.0 5 13239.0 6 8922.0 7 7511.0 8 7974.0 9 8319.0 10 8643.0 11 9373.0 12 10597.0 13 11702.0 14 12440.0 15 13165.0 16 13249.0 17 13393.0 18 13440.0 19 12776.0 20 12480.0 21 12801.0 22 15710.0 23 23455.0 24 37844.0 25 49603.0 26 58618.0 27 44114.0 28 40638.0 29 49549.0 30 59129.0 31 66996.0 32 84858.0 33 114025.0 34 161672.0 35 258176.0 36 434219.0 37 796194.0 38 1213800.0 39 628291.0 40 2704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.994519378934655 21.866055557398166 14.257413494662355 21.88201156900482 2 14.804899238967312 22.74584878528552 40.1756956711826 22.27355630456456 3 17.208237822156917 29.280633874471196 30.629952424452416 22.881175878919468 4 11.669967230410574 19.926500923612547 38.91554015367174 29.487991692305144 5 12.086115870074472 40.715673380615165 34.43027812368045 12.76793262562991 6 27.22522371667643 40.629800444769394 19.007198064756167 13.137777773798007 7 24.55968603417564 35.93250746644883 23.055135871347616 16.45267062802791 8 23.922909904868494 37.571035254616675 20.938914381645215 17.567140458869616 9 24.915713078657724 18.991645729697666 21.42321401592515 34.669427175719456 10-11 22.19462717813246 29.74904565221917 29.001617796613395 19.054709373034978 12-13 22.97181135940097 28.065163496354 28.528571988202966 20.43445315604206 14-15 20.979044772564574 29.438620438946362 26.125615411786985 23.45671937670208 16-17 20.13103998071401 32.32977611708942 26.89338077084198 20.645803131354587 18-19 20.217505248422732 31.244750038306186 28.657927074205652 19.879817639065433 20-21 22.027318353610127 30.139002665306236 27.889436296629004 19.94424268445463 22-23 21.633579977189825 30.153526566912266 27.934291479986257 20.278601975911652 24-25 21.185057658190253 30.22609648271105 27.952002974491297 20.636842884607397 26-27 20.826904892808088 30.297945346540327 27.992023062692628 20.883126697958957 28-29 20.811705213100293 30.177941830080062 28.234105605106645 20.776247351712996 30-31 21.04483761487945 30.459784202867258 27.899710310196767 20.595667872056524 32-33 20.780755928203977 30.348943579326885 28.231289357153116 20.63901113531602 34-35 21.130618099358603 30.04562698915037 28.07010651520722 20.753648396283804 36-37 21.21140479931654 30.0620693337596 27.8327382069777 20.893787659946156 38-39 20.978122058570197 30.18394314710448 28.071402594620228 20.766532199705097 40-41 20.901621683333826 29.93786261498606 28.242798627974302 20.91771707370581 42-43 20.827159319226098 30.066241696002326 28.17079791133821 20.935801073433364 44-45 21.034146077166184 30.092056090250207 28.036774076259885 20.83702375632372 46-47 21.044744360236542 30.180467729389854 27.968709091643785 20.806078818729816 48-49 21.003380781284875 29.978033093194355 27.975220738085383 21.043365387435387 50-51 20.90369235425754 29.953958626791305 28.02048288145656 21.121866137494592 52-53 20.904667069927918 30.023199461641752 28.045010492702566 21.02712297572776 54-55 21.0100519387481 30.227444703143192 27.909890749529865 20.85261260857885 56-57 21.012597247378437 30.019831715836293 27.76986434336291 21.197706693422358 58-59 21.019986386122127 29.83648906574567 27.99208957801792 21.15143497011428 60-61 21.121491477417415 30.111901326337026 27.739453514625772 21.02715368161979 62-63 20.908447850934238 30.02558598165778 27.985288620170166 21.080677547237812 64-65 21.043673229405364 29.911043123663795 27.961529063906198 21.083754583024646 66-67 20.887526405149035 29.918862474070547 27.888829355776483 21.304781765003938 68-69 21.20532286537169 30.118756237103273 27.656463149996263 21.019457747528776 70-71 21.128117967128187 29.849315510046754 27.82546009839249 21.19710642443257 72-73 21.007431173952682 30.00062002927344 27.982200906943905 21.009747889829974 74-75 21.036972767564492 30.066204396546777 27.85066107581948 21.04616176006925 76-77 21.06940111182722 29.950822252035508 27.80107547221764 21.178701163919634 78-79 21.073006336162464 29.976428266828435 27.943383069764945 21.007182327244156 80-81 21.034821460053294 29.947048904067348 27.92064667386412 21.097482962015242 82-83 20.939368679002023 29.92728477166639 28.13777443111788 20.99557211821371 84-85 20.911600985420186 29.856422738454402 27.909291015070554 21.322685261054858 86-87 21.1361501464553 29.85658708560673 27.85847519931084 21.148787568627135 88-89 20.999279907378604 29.78898812378877 27.965507194042072 21.246224774790555 90-91 20.95358303025934 29.798504254154786 27.82707841074793 21.42083430483794 92-93 21.069580496557926 29.80799224407351 27.893689993728866 21.2287372656397 94-95 20.96238019998037 29.762550594169145 28.01655859029476 21.25851061555572 96-97 20.992871906411075 29.86637300835724 27.84369656685933 21.297058518372356 98-99 20.916779076851853 29.845489024322124 27.87172623874793 21.36600566007809 100 20.92422982899865 29.94774741171811 27.78770211524585 21.340320644037387 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28165.0 1 20747.5 2 11802.0 3 8643.0 4 5648.5 5 3774.0 6 3235.0 7 3510.5 8 4163.5 9 4296.0 10 3268.0 11 2292.0 12 2100.5 13 2077.0 14 2136.0 15 2278.5 16 2534.5 17 3067.5 18 3879.5 19 4966.0 20 6724.5 21 9299.0 22 13541.0 23 20901.0 24 28959.0 25 36334.5 26 46426.5 27 59422.0 28 75871.5 29 91190.5 30 99633.0 31 108091.0 32 117382.0 33 126276.5 34 135031.0 35 140906.5 36 143542.0 37 144828.0 38 146231.0 39 146615.5 40 146808.0 41 144571.0 42 140181.0 43 138429.5 44 137731.0 45 134448.5 46 132500.5 47 131479.5 48 127655.5 49 124343.5 50 123317.5 51 119630.5 52 112294.0 53 106609.5 54 103787.0 55 101135.5 56 93172.5 57 82767.0 58 75318.5 59 68684.5 60 60545.5 61 51428.5 62 43765.0 63 35930.0 64 28341.5 65 22794.0 66 17909.5 67 13725.5 68 10664.5 69 8289.5 70 6264.0 71 4771.5 72 3715.5 73 2735.0 74 2079.0 75 1626.5 76 1157.0 77 872.5 78 680.5 79 523.0 80 388.5 81 268.0 82 221.0 83 189.0 84 151.0 85 133.5 86 118.0 87 111.0 88 97.0 89 84.0 90 75.5 91 58.5 92 51.0 93 40.0 94 34.5 95 33.0 96 25.0 97 23.0 98 23.0 99 26.0 100 37.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18641784708189188 2 0.18147444124974668 3 0.19339677296256746 4 0.18415864351154498 5 0.19787044339889798 6 0.0798102805841361 7 0.08095106654540037 8 0.06938662846748603 9 0.0632353316175316 10-11 0.07638792270034327 12-13 0.07553792531744047 14-15 0.07309977492964036 16-17 0.06957675946103008 18-19 0.07398332484081561 20-21 0.07572805631098452 22-23 0.07374845714290827 24-25 0.07344648438845597 26-27 0.07770765547906076 28-29 0.07443069188444867 30-31 0.08013462169077006 32-33 0.07646621193297905 34-35 0.07604121324152764 36-37 0.08731486274108051 38-39 0.08288592900911332 40-41 0.07794252317696812 42-43 0.0811300133628536 44-45 0.09139708701423209 46-47 0.08234908855675366 48-49 0.08685631152135663 50-51 0.08388132068119684 52-53 0.0824944828459344 54-55 0.08504447499464278 56-57 0.07862475791850851 58-59 0.0858161831449098 60-61 0.08221487844366374 62-63 0.07408398242563306 64-65 0.07689121062443044 66-67 0.07680173721570384 68-69 0.07569450378271204 70-71 0.07131030675510815 72-73 0.0686932095498548 74-75 0.07382674637554405 76-77 0.07920633507523148 78-79 0.07576160883925699 80-81 0.08375829474419777 82-83 0.08485434400109874 84-85 0.08674446976044838 86-87 0.08298658659393075 88-89 0.0853464477490951 90-91 0.0835122428701996 92-93 0.07400569319299728 94-95 0.07411753495390554 96-97 0.0733346426275477 98-99 0.08281882395256836 100 0.0901220909398779 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4470602.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.48907972981144 #Duplication Level Percentage of deduplicated Percentage of total 1 73.20326190317948 41.35184898130974 2 15.667335795770407 17.700667618420066 3 5.133258985501459 8.699192283172888 4 2.155273141104292 4.86997585229446 5 1.1543324590784214 3.260358915779512 6 0.6389509431803979 2.1656250463653435 7 0.4263342688509728 1.6858261353271433 8 0.280868109638968 1.2692784831165669 9 0.20967173861592353 1.0659747203786782 >10 1.0324763458022759 10.408177501507401 >50 0.06592415155349962 2.548360758733962 >100 0.02998010913778666 3.0607123939643177 >500 0.0015415240625073646 0.5607234713388838 >1k 7.509982975122883E-4 0.6582964659761469 >5k 0.0 0.0 >10k+ 3.952622618480881E-5 0.6949813723147791 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31126 0.6962373300061154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 2.2368352181652493E-5 6 0.0 0.0 0.0 0.0 2.2368352181652493E-5 7 0.0 0.0 0.0 0.0 2.2368352181652493E-5 8 0.0 0.0 0.0 0.0 2.2368352181652493E-5 9 0.0 0.0 0.0 0.0 2.2368352181652493E-5 10-11 0.0 0.0 0.0 0.0 2.2368352181652493E-5 12-13 0.0 0.0 0.0 0.0 2.2368352181652493E-5 14-15 0.0 1.1184176090826247E-5 0.0 0.0 2.2368352181652493E-5 16-17 0.0 2.2368352181652493E-5 0.0 0.0 2.2368352181652493E-5 18-19 0.0 2.2368352181652493E-5 0.0 2.2368352181652493E-5 2.2368352181652493E-5 20-21 0.0 2.2368352181652493E-5 0.0 3.355252827247874E-5 2.2368352181652493E-5 22-23 0.0 2.2368352181652493E-5 0.0 1.7894681745321995E-4 2.2368352181652493E-5 24-25 0.0 2.2368352181652493E-5 0.0 4.0263033926974485E-4 2.2368352181652493E-5 26-27 0.0 2.2368352181652493E-5 0.0 0.0011072334329917985 2.2368352181652493E-5 28-29 0.0 2.2368352181652493E-5 0.0 0.002639465557434994 2.2368352181652493E-5 30-31 0.0 2.2368352181652493E-5 0.0 0.00622958608259022 2.2368352181652493E-5 32-33 0.0 2.2368352181652493E-5 0.0 0.013040749321903404 2.2368352181652493E-5 34-35 0.0 2.2368352181652493E-5 0.0 0.022189405364199274 2.2368352181652493E-5 36-37 0.0 2.2368352181652493E-5 0.0 0.03812685629362668 2.2368352181652493E-5 38-39 0.0 2.2368352181652493E-5 0.0 0.060909022990639744 2.2368352181652493E-5 40-41 0.0 2.2368352181652493E-5 0.0 0.08530171104473178 2.2368352181652493E-5 42-43 0.0 2.2368352181652493E-5 0.0 0.10960492569009722 2.2368352181652493E-5 44-45 0.0 2.2368352181652493E-5 0.0 0.14097653962486484 2.2368352181652493E-5 46-47 0.0 2.2368352181652493E-5 0.0 0.17957313131430622 2.2368352181652493E-5 48-49 0.0 2.2368352181652493E-5 0.0 0.22230786815735332 2.2368352181652493E-5 50-51 0.0 2.2368352181652493E-5 0.0 0.2675366762686546 2.2368352181652493E-5 52-53 0.0 2.2368352181652493E-5 0.0 0.30963391507452465 2.2368352181652493E-5 54-55 0.0 2.2368352181652493E-5 0.0 0.35898968416334087 2.2368352181652493E-5 56-57 0.0 2.2368352181652493E-5 0.0 0.418780289544898 2.2368352181652493E-5 58-59 0.0 2.2368352181652493E-5 0.0 0.49035901652618596 2.2368352181652493E-5 60-61 0.0 2.2368352181652493E-5 0.0 0.5670712803331632 2.2368352181652493E-5 62-63 0.0 2.2368352181652493E-5 0.0 0.6451927503275845 2.2368352181652493E-5 64-65 0.0 2.2368352181652493E-5 0.0 0.7268484199667069 2.2368352181652493E-5 66-67 0.0 6.710505654495748E-5 0.0 0.8237033849132622 2.2368352181652493E-5 68-69 0.0 6.710505654495748E-5 0.0 0.9399181586730379 2.2368352181652493E-5 70-71 0.0 6.710505654495748E-5 0.0 1.071030254985794 2.2368352181652493E-5 72-73 0.0 6.710505654495748E-5 0.0 1.2050726054343466 2.2368352181652493E-5 74-75 0.0 6.710505654495748E-5 0.0 1.3457583564808497 2.2368352181652493E-5 76-77 0.0 6.710505654495748E-5 0.0 1.4996638036667098 2.2368352181652493E-5 78-79 0.0 6.710505654495748E-5 0.0 1.681932321418905 2.2368352181652493E-5 80-81 0.0 6.710505654495748E-5 0.0 1.883661305569138 2.2368352181652493E-5 82-83 0.0 6.710505654495748E-5 0.0 2.1014395824097067 2.2368352181652493E-5 84-85 0.0 6.710505654495748E-5 0.0 2.320996143248717 2.2368352181652493E-5 86-87 0.0 6.710505654495748E-5 0.0 2.5545329242012595 2.2368352181652493E-5 88 0.0 6.710505654495748E-5 0.0 2.7463415441589296 2.2368352181652493E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6850 0.0 49.047913 1 GTATCAA 11505 0.0 35.705944 1 TCAACGC 13230 0.0 29.869648 4 ATCAACG 13350 0.0 29.568897 3 TATCAAC 14405 0.0 28.056873 2 CAACGCA 14165 0.0 28.030237 5 GTACATG 23570 0.0 27.07154 1 TACATGG 23575 0.0 26.343992 2 AACGCAG 15230 0.0 25.982746 6 ACATGGG 24260 0.0 24.5332 3 TACCTGG 3620 0.0 24.044872 2 CATGGGT 10065 0.0 24.024813 4 GTGGTAT 3275 0.0 22.989079 1 CGCAGAG 17175 0.0 22.410435 8 ACGCAGA 17625 0.0 22.23897 7 ATGGGTG 6100 0.0 20.822596 5 GTACCTG 4360 0.0 20.721807 1 TGGTATC 3360 0.0 20.444332 2 GCAGAGT 18690 0.0 19.83973 9 CATGGGG 11215 0.0 19.58972 4 >>END_MODULE