##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431417_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4470602
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64027506810045	34.0	31.0	34.0	31.0	34.0
2	32.76086442049639	34.0	31.0	34.0	31.0	34.0
3	32.86933437599679	34.0	31.0	34.0	31.0	34.0
4	36.33603013643353	37.0	37.0	37.0	35.0	37.0
5	36.23597470765682	37.0	37.0	37.0	35.0	37.0
6	36.27887989134349	37.0	37.0	37.0	35.0	37.0
7	36.23895350111685	37.0	37.0	37.0	35.0	37.0
8	36.25126548952468	37.0	37.0	37.0	35.0	37.0
9	38.04953672905796	39.0	39.0	39.0	35.0	39.0
10-11	38.03010701914418	39.0	39.0	39.0	35.0	39.0
12-13	38.01001833757512	39.0	39.0	39.0	35.0	39.0
14-15	39.47440411828205	41.0	39.0	41.0	36.0	41.0
16-17	39.502786649314785	41.0	39.0	41.0	36.5	41.0
18-19	39.57388993697046	41.0	40.0	41.0	37.0	41.0
20-21	39.56522477286057	41.0	40.0	41.0	37.0	41.0
22-23	39.50158110697396	41.0	39.5	41.0	36.0	41.0
24-25	39.4487762498205	41.0	39.0	41.0	36.0	41.0
26-27	39.330925119256875	41.0	39.0	41.0	36.0	41.0
28-29	39.248149689907535	41.0	39.0	41.0	35.0	41.0
30-31	39.06222786103527	41.0	39.0	41.0	35.0	41.0
32-33	38.828697343221336	41.0	38.0	41.0	35.0	41.0
34-35	38.68973507371043	41.0	38.0	41.0	35.0	41.0
36-37	38.546886191166195	41.0	38.0	41.0	35.0	41.0
38-39	38.405831138625175	41.0	38.0	41.0	35.0	41.0
40-41	38.26644722567565	40.0	38.0	41.0	34.5	41.0
42-43	38.09302930567293	40.0	38.0	41.0	34.0	41.0
44-45	37.84321127221793	40.0	38.0	41.0	33.5	41.0
46-47	37.459852722295565	40.0	38.0	41.0	33.0	41.0
48-49	37.11252097592226	40.0	37.0	41.0	32.0	41.0
50-51	36.94187997500113	40.0	37.0	41.0	31.5	41.0
52-53	36.880806768305476	40.0	37.0	41.0	31.5	41.0
54-55	36.73389377985336	40.0	36.0	41.0	31.0	41.0
56-57	36.579167302300675	40.0	36.0	41.0	31.0	41.0
58-59	36.39445548943968	40.0	35.5	41.0	31.0	41.0
60-61	36.2347800139668	39.0	35.0	41.0	31.0	41.0
62-63	36.066625702757705	39.0	35.0	41.0	31.0	41.0
64-65	35.82821295655484	39.0	35.0	41.0	30.0	41.0
66-67	35.55784813767811	38.5	35.0	41.0	30.0	41.0
68-69	35.26012559382383	37.5	35.0	40.5	29.5	41.0
70-71	34.95625768073293	37.0	35.0	40.0	29.0	41.0
72-73	34.63610303489328	36.5	35.0	39.5	29.0	41.0
74-75	34.30068892287884	36.0	35.0	39.0	29.0	41.0
76-77	33.97117994399859	36.0	35.0	39.0	29.0	41.0
78-79	33.65645297881583	35.0	34.0	37.5	28.5	39.5
80-81	33.39734850026909	35.0	34.0	37.0	29.0	39.0
82-83	33.199396747910015	35.0	34.0	37.0	29.0	39.0
84-85	32.94181991597552	35.0	34.0	36.0	29.0	38.0
86-87	32.7058801029481	35.0	34.0	36.0	29.0	37.0
88-89	32.47803796893572	35.0	34.0	36.0	28.0	37.0
90-91	32.302210418194235	35.0	34.0	35.0	27.5	36.5
92-93	32.146106944881254	35.0	34.0	35.0	27.0	36.0
94-95	32.01016205423789	35.0	34.0	35.0	27.0	36.0
96-97	31.871194640005974	35.0	34.0	35.0	27.0	36.0
98-99	31.75849270411457	35.0	34.0	35.0	26.0	36.0
100	31.657219318561573	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.027603214272858168
1101	2	0.0198984439224148
1101	3	0.02375693449609173
1101	4	0.05997639140841926
1101	5	-0.00233606185881996
1101	6	0.07724005418653235
1101	7	0.07426206162544702
1101	8	0.06638594660135766
1101	9	0.040975295757881725
1101	10-11	0.09869078364915396
1101	12-13	0.02993049902224243
1101	14-15	0.02698064789935728
1101	16-17	0.03527088665454414
1101	18-19	0.03824090052620477
1101	20-21	0.08756454902158595
1101	22-23	0.08822671131148496
1101	24-25	0.09586711859000019
1101	26-27	0.12436925292837486
1101	28-29	0.0461889640255535
1101	30-31	0.0415868949807745
1101	32-33	0.005667074979889719
1101	34-35	0.039687156376423616
1101	36-37	0.01198569115443604
1101	38-39	-0.020610743474875903
1101	40-41	-0.08803929469950589
1101	42-43	-0.05286664639599792
1101	44-45	-0.07589246647525272
1101	46-47	-0.11167638554581316
1101	48-49	-0.14022927838634303
1101	50-51	-0.18710822831076968
1101	52-53	-0.11069827094136997
1101	54-55	-0.13773523857555858
1101	56-57	-0.13968724655941855
1101	58-59	-0.1786875648735844
1101	60-61	-0.134084545083617
1101	62-63	-0.17324976762204614
1101	64-65	-0.1588953132100599
1101	66-67	-0.15505720006584056
1101	68-69	-0.184067756232686
1101	70-71	-0.11156671572760501
1101	72-73	-0.17321212341457226
1101	74-75	-0.1950005069516152
1101	76-77	-0.1957137563511253
1101	78-79	-0.169699856895015
1101	80-81	-0.14638581270398987
1101	82-83	-0.11477638742825746
1101	84-85	-0.08885911124436063
1101	86-87	-0.0894833820184644
1101	88-89	-0.062426450095230734
1101	90-91	-0.06793603000987503
1101	92-93	-0.054972280175455523
1101	94-95	-0.07057473037524531
1101	96-97	-0.04215021064241853
1101	98-99	-0.06132988120330651
1101	100	-0.021559483182358008
1102	1	-0.06457052692088894
1102	2	-0.02583376484840727
1102	3	-0.008349308555430923
1102	4	-0.015523850705683628
1102	5	-0.03687918962937431
1102	6	-0.048447185388354796
1102	7	-0.05696783091580215
1102	8	-0.008031448139988129
1102	9	-0.05802753227901292
1102	10-11	-0.026505852641690808
1102	12-13	-0.06746741876872164
1102	14-15	-0.05529795766751988
1102	16-17	-0.060793879076840085
1102	18-19	-0.05815281886479795
1102	20-21	-0.05892361843101668
1102	22-23	-0.0348478024577048
1102	24-25	-0.06548610662767373
1102	26-27	-0.0056855477356307915
1102	28-29	-0.06365029271879763
1102	30-31	-0.03324465762894846
1102	32-33	-0.03166407918789105
1102	34-35	-0.026427401430630937
1102	36-37	0.005222905048867688
1102	38-39	-0.03764662375991179
1102	40-41	-0.06342955298134001
1102	42-43	-0.05041121853587782
1102	44-45	-0.0381898812731265
1102	46-47	-0.11120669526615501
1102	48-49	-0.06173750652407506
1102	50-51	-0.021001001835259103
1102	52-53	0.012453242597480596
1102	54-55	0.021276977446213152
1102	56-57	2.3285162775721346E-4
1102	58-59	-0.005122600267696953
1102	60-61	-0.10925290932080145
1102	62-63	-0.05078788430969183
1102	64-65	-0.0910037736775493
1102	66-67	-0.1259549181507822
1102	68-69	-0.11552476760497399
1102	70-71	-0.08452658909097721
1102	72-73	-0.09223081706276304
1102	74-75	-0.07245355801897801
1102	76-77	-0.10287488737741057
1102	78-79	-0.08152626482250014
1102	80-81	-0.09622158533348824
1102	82-83	-0.0740565088761258
1102	84-85	-0.09596421094077812
1102	86-87	-0.09077972497289011
1102	88-89	-0.10901157406125606
1102	90-91	-0.10399742249721555
1102	92-93	-0.08044979377583417
1102	94-95	-0.11888550288693622
1102	96-97	-0.11690659285064697
1102	98-99	-0.09534497293642374
1102	100	-0.05463764737258714
1103	1	-0.11547667593486466
1103	2	-0.1182672678001424
1103	3	-0.07801299278915508
1103	4	-0.026714518081533356
1103	5	-0.045018153947708583
1103	6	-0.00612992687004521
1103	7	-0.01980183023930948
1103	8	-0.015040996402404971
1103	9	-0.010696622301189507
1103	10-11	0.023435747044864286
1103	12-13	-0.007087908056917058
1103	14-15	0.041879147651762594
1103	16-17	-0.0184042192763485
1103	18-19	4.5577186594414343E-4
1103	20-21	-0.04654766845602154
1103	22-23	-0.04703658303306213
1103	24-25	-0.07315449007816
1103	26-27	-0.09084583925790923
1103	28-29	-0.042397110571201324
1103	30-31	-0.04450992359448236
1103	32-33	-0.024731216957285085
1103	34-35	0.026054203328946812
1103	36-37	0.021686204039660595
1103	38-39	-0.020772057470644256
1103	40-41	-0.020715666725791948
1103	42-43	0.019287485286156425
1103	44-45	-0.0297865671291504
1103	46-47	-0.04757499092047368
1103	48-49	0.02448074736079775
1103	50-51	-0.08915256771211943
1103	52-53	-0.030314793554822472
1103	54-55	0.017446287679803163
1103	56-57	-0.04349643789070967
1103	58-59	-0.07771186210833747
1103	60-61	-0.0709244231104833
1103	62-63	-0.09834993730352437
1103	64-65	-0.08672266333962142
1103	66-67	-0.04206826845557288
1103	68-69	-0.04501454318984344
1103	70-71	-0.019685981090496796
1103	72-73	0.04358936023523796
1103	74-75	-0.009444350888550446
1103	76-77	-0.02246314216455403
1103	78-79	0.03582754796607901
1103	80-81	0.026433121937778026
1103	82-83	0.01243410127359823
1103	84-85	-0.019023868251437648
1103	86-87	0.0407487949111669
1103	88-89	0.035860617487927016
1103	90-91	0.044022741644397456
1103	92-93	0.05658780174352529
1103	94-95	0.03346545820850011
1103	96-97	-0.015205423649589989
1103	98-99	-0.011745785995131541
1103	100	0.021009167856785638
1104	1	-0.1915831788234641
1104	2	-0.15682228542204513
1104	3	-0.14665156987236827
1104	4	-0.10368585913498407
1104	5	-0.11567323343301439
1104	6	-0.15761285408576953
1104	7	-0.1347160416218003
1104	8	-0.17616937745545158
1104	9	-0.12861784176793378
1104	10-11	-0.21038835511828324
1104	12-13	-0.20995749803308428
1104	14-15	-0.2410355199301435
1104	16-17	-0.20406565391714793
1104	18-19	-0.22397664402627981
1104	20-21	-0.17340550465119264
1104	22-23	-0.16133032600298236
1104	24-25	-0.17184940353452305
1104	26-27	-0.19068943942625083
1104	28-29	-0.20873822118280572
1104	30-31	-0.13860724560293392
1104	32-33	-0.1738489257090592
1104	34-35	-0.2248691485244123
1104	36-37	-0.18191201561290882
1104	38-39	-0.201597366329068
1104	40-41	-0.21715887986901095
1104	42-43	-0.1530893215998006
1104	44-45	-0.1848341636680999
1104	46-47	-0.2233535805465081
1104	48-49	-0.1922109535584866
1104	50-51	-0.28925736596750085
1104	52-53	-0.24336236244531761
1104	54-55	-0.20539000186644785
1104	56-57	-0.2034004977729964
1104	58-59	-0.22249400163570243
1104	60-61	-0.23549992770132633
1104	62-63	-0.2561867838065197
1104	64-65	-0.2515879715281031
1104	66-67	-0.30486121905816077
1104	68-69	-0.3666241587886745
1104	70-71	-0.3442635646320156
1104	72-73	-0.3269323553616559
1104	74-75	-0.254950574049289
1104	76-77	-0.2770090124034681
1104	78-79	-0.27380834854684366
1104	80-81	-0.279063854943594
1104	82-83	-0.2822397573572246
1104	84-85	-0.3477924648241526
1104	86-87	-0.2775187213781578
1104	88-89	-0.2901203773936558
1104	90-91	-0.31354814804812037
1104	92-93	-0.3381581023647442
1104	94-95	-0.3528261483870381
1104	96-97	-0.35049747235446205
1104	98-99	-0.4409429468184456
1104	100	-0.4153933116773807
1105	1	-0.12636772854208544
1105	2	-0.11176724910532698
1105	3	-0.12562012129368583
1105	4	-0.0597854179873778
1105	5	-0.13003147171858842
1105	6	-0.04989175330420892
1105	7	-0.029633971679864146
1105	8	-0.06429740016238839
1105	9	-0.008027080554349197
1105	10-11	-0.06844254366820479
1105	12-13	-0.05182403525908086
1105	14-15	-0.10074653055836791
1105	16-17	-0.11802236125187449
1105	18-19	-0.14696042108968754
1105	20-21	-0.10072399955983968
1105	22-23	-0.16770581049776467
1105	24-25	-0.1452952743558953
1105	26-27	-0.11631275870990976
1105	28-29	-0.11544718209523097
1105	30-31	-0.10846775017400745
1105	32-33	-0.12072547934730693
1105	34-35	-0.09815969259555146
1105	36-37	-0.11512197325730256
1105	38-39	-0.10245227199827411
1105	40-41	-0.17582994174985345
1105	42-43	-0.22439309058749046
1105	44-45	-0.13771049045790562
1105	46-47	-0.11723995361319339
1105	48-49	-0.2614196984125883
1105	50-51	-0.2736796894499065
1105	52-53	-0.21856485127344172
1105	54-55	-0.14055338235010595
1105	56-57	-0.15876983066572592
1105	58-59	-0.1894143744567245
1105	60-61	-0.18084354121702972
1105	62-63	-0.2619872719295486
1105	64-65	-0.2669706586144045
1105	66-67	-0.23366703893783836
1105	68-69	-0.1546675019783592
1105	70-71	-0.18089471489169995
1105	72-73	-0.16138011491620574
1105	74-75	-0.17009685498769045
1105	76-77	-0.24132275034728679
1105	78-79	-0.1302611742469466
1105	80-81	-0.10351765185778561
1105	82-83	-0.14620141485637106
1105	84-85	-0.15003743598381902
1105	86-87	-0.12878934369666695
1105	88-89	-0.07669749006298332
1105	90-91	-0.07260152830983202
1105	92-93	-0.12896783573805237
1105	94-95	-0.10101440354919689
1105	96-97	-0.05588501039734339
1105	98-99	-0.07850154720241065
1105	100	-0.0896535203431732
1106	1	-0.15940077518540363
1106	2	-0.1265550698381972
1106	3	-0.10728132687324887
1106	4	-0.044081892930222466
1106	5	-0.10121495352370147
1106	6	-0.13677679132908338
1106	7	-0.06839804082621725
1106	8	-0.056027016018816767
1106	9	-0.1370833485626335
1106	10-11	-0.12690863219844317
1106	12-13	-0.09227094867760144
1106	14-15	-0.1373248734249657
1106	16-17	-0.1710127913375743
1106	18-19	-0.12461479889711313
1106	20-21	-0.0999739163715816
1106	22-23	-0.10985871177460638
1106	24-25	-0.10776141181465704
1106	26-27	-0.14075397439097515
1106	28-29	-0.13111371835078245
1106	30-31	-0.20248682664891504
1106	32-33	-0.13190511314744668
1106	34-35	-0.13807046332521367
1106	36-37	-0.12325865677214409
1106	38-39	-0.12532446072751213
1106	40-41	-0.21137486428390417
1106	42-43	-0.20676485640655784
1106	44-45	-0.18446548092464354
1106	46-47	-0.18489695523758343
1106	48-49	-0.1447112573048983
1106	50-51	-0.1747839879287767
1106	52-53	-0.21034948736308934
1106	54-55	-0.1522792223272873
1106	56-57	-0.11628295316191384
1106	58-59	-0.0834733296356518
1106	60-61	-0.11958647947487577
1106	62-63	-0.15246360094839417
1106	64-65	-0.15434816427676168
1106	66-67	-0.20816979053429918
1106	68-69	-0.24268469460726294
1106	70-71	-0.17213604445344544
1106	72-73	-0.18684653931840955
1106	74-75	-0.22983386704495956
1106	76-77	-0.2214346660826081
1106	78-79	-0.14614476995222958
1106	80-81	-0.17271948420211913
1106	82-83	-0.2346910553603081
1106	84-85	-0.1484856607260241
1106	86-87	-0.1634822079866538
1106	88-89	-0.19698724152357272
1106	90-91	-0.2254933995650248
1106	92-93	-0.1564216163054688
1106	94-95	-0.19315431244855574
1106	96-97	-0.22802570293297464
1106	98-99	-0.21452786510025845
1106	100	-0.18909014044586314
1107	1	-0.22529222065850263
1107	2	-0.16517646700238942
1107	3	-0.14280691875676865
1107	4	-0.14283799185651702
1107	5	-0.21650028064780713
1107	6	-0.22218046594218777
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2216	12-13	0.0816587874472603
2216	14-15	0.08836129549381866
2216	16-17	0.08124389753375993
2216	18-19	0.0749089083953578
2216	20-21	0.07403992620611888
2216	22-23	-0.013979375722627196
2216	24-25	4.585304234225873E-4
2216	26-27	0.004542401787134054
2216	28-29	0.04593950418433934
2216	30-31	-0.03891973948174865
2216	32-33	0.030952591578802924
2216	34-35	0.08637086128207017
2216	36-37	0.07905615147537759
2216	38-39	0.021299675280261
2216	40-41	0.04410770491399063
2216	42-43	0.11310975952142144
2216	44-45	0.07698809065490764
2216	46-47	0.1567984685315551
2216	48-49	0.17381692317346875
2216	50-51	0.13732797468003355
2216	52-53	0.07488531167030743
2216	54-55	0.08385247578040378
2216	56-57	0.06656895674089469
2216	58-59	0.1000078348592055
2216	60-61	0.037165700182583805
2216	62-63	-0.011509240246716956
2216	64-65	0.01977356816019693
2216	66-67	0.041586755954178045
2216	68-69	0.040041122661513384
2216	70-71	0.05386551925260363
2216	72-73	0.058773167682723226
2216	74-75	0.0524027760140271
2216	76-77	0.044032311222167664
2216	78-79	0.024440989235628763
2216	80-81	0.09069770045937986
2216	82-83	0.11806284079569451
2216	84-85	0.139369723883064
2216	86-87	0.08081621434072872
2216	88-89	0.10950073942260019
2216	90-91	0.0973064783138966
2216	92-93	0.08260537630820153
2216	94-95	0.04476280844941627
2216	96-97	0.047045791962801786
2216	98-99	0.03275695382438926
2216	100	0.015899709967804654
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58.0
3	332.0
4	105.0
5	162.0
6	399.0
7	854.0
8	1522.0
9	2733.0
10	3916.0
11	5794.0
12	8682.0
13	12782.0
14	14174.0
15	18657.0
16	24623.0
17	37709.0
18	44900.0
19	23171.0
20	14110.0
21	12679.0
22	12730.0
23	12630.0
24	13480.0
25	15478.0
26	18820.0
27	23629.0
28	29597.0
29	37574.0
30	46947.0
31	59704.0
32	76016.0
33	99113.0
34	140362.0
35	235608.0
36	379242.0
37	778787.0
38	1386552.0
39	868491.0
40	8480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.788547721852154	20.82990518398816	14.879264271055773	22.502282823103915
2	14.299292501156497	21.963154941978296	40.647808382076214	23.08974417478899
3	16.301119948176563	28.645043444182882	32.20701012221277	22.84682648542779
4	11.117518401324922	18.771700097660226	39.53422827619188	30.576553224822966
5	11.310033866276434	39.05457745257479	35.48893947060473	14.146449210544043
6	26.97468931477237	40.10039363826169	18.675941181970572	14.248975864995364
7	23.90830138759836	35.478242080149386	23.651736388074802	16.96172014417745
8	24.90053912202428	35.08004067461161	21.169721661646463	18.849698541717647
9	25.845428836078195	15.953559711546614	22.305927100181833	35.895084352193365
10-11	22.765770694863914	27.60794854921105	29.5178031951849	20.10847756074014
12-13	24.475028695803015	26.013859656245366	29.69771517322669	19.813396474724936
14-15	21.761532339492533	26.874154755891936	28.696839933413887	22.66747297120164
16-17	19.29665177083534	30.145179552999796	29.297228874321625	21.260939801843243
18-19	19.23933459476012	27.63881305391067	33.479842410379455	19.642009940949748
20-21	20.841868723719983	26.628863405867936	33.33305000087237	19.196217869539716
22-23	20.3918355039776	26.66225711736041	33.52145869441924	19.424448684242755
24-25	20.046069883916015	26.79441537986125	33.43626509151625	19.723249644706485
26-27	19.735943396342478	26.897160992794134	33.433618280521756	19.933277330341635
28-29	19.73768150823738	26.790846231688125	33.658398119624316	19.813074140450176
30-31	19.992998683058993	27.052551817853914	33.313932452949416	19.640517046137674
32-33	19.765509312074155	26.988831326864315	33.519753765764314	19.725905595297213
34-35	20.084888788975867	26.80179530282034	33.2060560713379	19.907259836865894
36-37	20.23029895513671	26.889554878977627	32.82881899870195	20.051327167183718
38-39	20.03560186750987	27.032992793731065	32.96870274632752	19.96270259243154
40-41	20.023278785772092	26.943679072624356	32.885820094600405	20.14722204700315
42-43	20.049631216218682	27.092105744374855	32.61649763727182	20.241765402134646
44-45	20.328220256115767	27.20204724404165	32.22072987815605	20.24900262168653
46-47	20.479473251146633	27.49206565079111	31.643416025187708	20.385045072874554
48-49	20.622485582730793	27.51482549240945	31.092918647155017	20.769770277704737
50-51	20.579031668006724	27.699666564852986	30.849489591349855	20.871812175790435
52-53	20.5387407230862	27.892763646543333	30.82422199299486	20.744273637375606
54-55	20.65189977686263	28.117724282917738	30.644004800736013	20.586371139483617
56-57	20.567393813301635	27.829629300725273	30.63729522612686	20.96568165984623
58-59	20.560592442939093	27.58462336156386	30.918803715138143	20.9359804803589
60-61	20.654927446141304	27.743420762722003	30.792503636584478	20.80914815455221
62-63	20.47406769155335	27.60976686724633	31.081515153752015	20.8346502874483
64-65	20.48344177689973	27.590264505669126	31.075289093368834	20.851004624062302
66-67	20.439077157980137	27.618524303664334	30.912346739719066	21.030051798636464
68-69	20.783979734207193	27.817484838402113	30.503764885487644	20.89477054190305
70-71	20.690927087323534	27.5936440121125	30.595743847984185	21.11968505257978
72-73	20.640311242930608	27.75995078056226	30.58421911283999	21.015518863667147
74-75	20.648804901652998	27.89725971399419	30.415818015294903	21.038117369057908
76-77	20.662057404837512	27.800452023503077	30.35934615856075	21.17814441309866
78-79	20.65968633935226	27.821008713889345	30.464657255773542	21.05464769098485
80-81	20.65764350301796	27.79409685749104	30.423930471474765	21.124329168016235
82-83	20.486859064263914	27.78383969342365	30.64582441381723	21.08347682849521
84-85	20.4733450254214	27.80237396138211	30.35780093453941	21.366480078657084
86-87	20.602528637955025	27.77581615697271	30.31230556606579	21.309349639006474
88-89	20.456290970242677	27.711813309625565	30.415308285016163	21.416587435115595
90-91	20.384986402672155	27.672952809471322	30.33719024347689	21.604870544379633
92-93	20.428598606456728	27.764983542839783	30.31854159217447	21.487876258529017
94-95	20.300615145753902	27.55828595771923	30.473050786740334	21.668048109786533
96-97	20.299360331803413	27.73561216293387	30.274488944322677	21.690538560940045
98-99	20.270492594424734	27.61739544348172	30.303822688062375	21.80828927403117
100	20.24772988830346	27.7018159775153	30.22443321302869	21.82602092115255
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6951.0
1	3936.0
2	4311.5
3	7263.0
4	4226.0
5	2094.0
6	4742.0
7	12122.5
8	21269.0
9	23391.0
10	14969.0
11	6941.0
12	5541.5
13	5943.5
14	6525.0
15	6994.5
16	7632.0
17	8299.5
18	9128.0
19	10345.5
20	12024.0
21	14412.0
22	18375.5
23	25249.0
24	32408.0
25	38902.0
26	48230.0
27	60272.0
28	76029.5
29	90429.0
30	98327.0
31	106395.5
32	114993.5
33	123504.0
34	132020.0
35	138085.0
36	140858.5
37	142251.5
38	143628.0
39	143918.5
40	144313.0
41	142124.0
42	137985.0
43	136847.5
44	136473.5
45	133553.5
46	131998.5
47	130925.0
48	126649.0
49	122844.0
50	122278.5
51	118390.5
52	110645.0
53	105015.5
54	101161.0
55	97690.5
56	89983.5
57	79576.5
58	72494.5
59	66390.0
60	58410.0
61	49897.5
62	42741.0
63	34595.0
64	26331.5
65	20872.0
66	16073.5
67	11841.5
68	8953.5
69	6831.5
70	4971.5
71	3583.0
72	2645.0
73	1873.0
74	1417.5
75	1036.0
76	632.0
77	434.5
78	315.5
79	174.5
80	79.0
81	34.0
82	19.5
83	8.5
84	8.5
85	6.0
86	0.5
87	1.0
88	1.0
89	2.0
90	2.5
91	2.0
92	1.5
93	1.0
94	1.0
95	0.5
96	0.0
97	1.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005815771567229649
2	0.004607880549420414
3	8.499973829027947E-4
4	0.0
5	0.047980115429644594
6	0.0
7	0.0
8	0.0
9	2.2368352181652493E-5
10-11	0.0
12-13	1.1184176090826247E-5
14-15	0.0
16-17	0.0
18-19	6.710505654495748E-5
20-21	0.0
22-23	3.355252827247874E-5
24-25	5.592088045413123E-5
26-27	1.677626413623937E-4
28-29	1.3421011308991496E-4
30-31	3.243411066339611E-4
32-33	0.0013868378352624546
34-35	0.0010513125525376673
36-37	0.0014539428918074122
38-39	0.0011184176090826246
40-41	1.7894681745321995E-4
42-43	6.37498037177096E-4
44-45	1.4539428918074123E-4
46-47	1.9013099354404619E-4
48-49	1.9013099354404619E-4
50-51	3.914461631789186E-4
52-53	6.37498037177096E-4
54-55	8.388132068119685E-4
56-57	0.002046704224621203
58-59	6.263138610862699E-4
60-61	0.001364469483080802
62-63	0.001319732778717497
64-65	0.0016552580614422846
66-67	4.697353958147024E-4
68-69	3.243411066339611E-4
70-71	3.243411066339611E-4
72-73	2.348676979073512E-4
74-75	6.37498037177096E-4
76-77	8.499973829027947E-4
78-79	8.499973829027947E-4
80-81	7.605239741761847E-4
82-83	0.0015210479483523695
84-85	0.0012861802504450183
86-87	0.002069072576802856
88-89	0.0012414435460817133
90-91	0.001219075193900061
92-93	0.0012414435460817133
94-95	7.940765024486634E-4
96-97	6.710505654495748E-5
98-99	3.914461631789186E-4
100	1.5657846527156747E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4470602.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.74915665094944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.05139818007396	36.25100778038682
2	17.20863701301174	17.810649050713426
3	5.845122448882103	9.074404717535433
4	2.5193533397090286	5.214976425427807
5	1.2862689488788412	3.328166666539162
6	0.7486235070726857	2.324438108405254
7	0.47815436912812614	1.7320859745943114
8	0.312022564848763	1.2917523669591733
9	0.24422051049526397	1.1374384909494832
>10	1.1836901281058274	10.961415192122837
>50	0.07881177923648476	2.8078548623149264
>100	0.03989319756049914	3.705954830802951
>500	0.002247826371508322	0.7233955203704836
>1k	0.0012103673750987057	0.8374365133219852
>5k	2.1613703126753937E-4	0.8229185013240223
>10k+	1.2968221876052364E-4	1.9761049982320245
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37503	0.8388803118685135	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30795	0.6888334054339885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20040	0.448261777720316	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9209	0.2059901552408378	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8629	0.19301651097547937	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6514	0.14570744611128436	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6254	0.1398916745440547	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6181	0.13825878483479406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	1.1184176090826247E-5	0.0	0.0	0.0
16-17	0.0	2.2368352181652493E-5	0.0	0.0	0.0
18-19	0.0	3.355252827247874E-5	0.0	2.2368352181652493E-5	0.0
20-21	0.0	4.4736704363304987E-5	0.0	5.592088045413123E-5	0.0
22-23	0.0	4.4736704363304987E-5	0.0	2.0131516963487243E-4	0.0
24-25	0.0	6.710505654495748E-5	0.0	4.2499869145139733E-4	0.0
26-27	0.0	6.710505654495748E-5	0.0	0.0011072334329917985	0.0
28-29	0.0	6.710505654495748E-5	0.0	0.0026506497335258205	0.0
30-31	0.0	6.710505654495748E-5	0.0	0.0063078753152260025	0.0
32-33	0.0	6.710505654495748E-5	0.0	0.013264432843719929	0.0
34-35	0.0	6.710505654495748E-5	0.0	0.022502562294742408	0.0
36-37	0.0	6.710505654495748E-5	0.0	0.03863014421771385	0.0
38-39	0.0	6.710505654495748E-5	0.0	0.06163599443654344	0.0
40-41	0.0	6.710505654495748E-5	0.0	0.08599512996236301	0.0
42-43	0.0	6.710505654495748E-5	0.0	0.11061150153827158	0.0
44-45	0.0	6.710505654495748E-5	0.0	0.14230745657967317	0.0
46-47	0.0	6.710505654495748E-5	0.0	0.18100470585393197	0.0
48-49	0.0	6.710505654495748E-5	0.0	0.22415325721233964	0.0
50-51	0.0	6.710505654495748E-5	0.0	0.2700307475369089	0.0
52-53	0.0	6.710505654495748E-5	0.0	0.31283258943650094	0.0
54-55	0.0	6.710505654495748E-5	0.0	0.3626692780972227	0.0
56-57	0.0	6.710505654495748E-5	0.0	0.42343290679868173	0.0
58-59	0.0	6.710505654495748E-5	0.0	0.49577215775414585	0.0
60-61	0.0	6.710505654495748E-5	0.0	0.5736587600506599	0.0
62-63	0.0	6.710505654495748E-5	0.0	0.6529657527107088	0.0
64-65	0.0	7.828923263578373E-5	0.0	0.7356727349023688	0.0
66-67	0.0	8.947340872660997E-5	0.0	0.83382506427546	0.0
68-69	0.0	8.947340872660997E-5	0.0	0.9513148341095897	0.0
70-71	0.0	8.947340872660997E-5	0.0	1.0839703467228798	0.0
72-73	0.0	8.947340872660997E-5	0.0	1.2195113767676031	0.0
74-75	0.0	8.947340872660997E-5	0.0	1.3623109370952726	0.0
76-77	0.0	1.1184176090826247E-4	0.0	1.5184755878514795	0.0
78-79	0.0	1.1184176090826247E-4	0.0	1.7036519913872896	0.0
80-81	0.0	1.1184176090826247E-4	0.0	1.9086803074843166	0.0
82-83	0.0	1.1184176090826247E-4	0.0	2.1297802846238607	0.0
84-85	0.0	1.1184176090826247E-4	0.0	2.3533072279751135	0.0
86-87	0.0	1.1184176090826247E-4	0.0	2.5911051800182614	0.0
88	0.0	1.1184176090826247E-4	0.0	2.786425631268451	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14115	0.0	62.99982	1
GTATCAA	26355	0.0	43.982765	1
GTGGTAT	4220	0.0	34.098686	1
ATCAACG	34060	0.0	33.850163	3
TCAACGC	34410	0.0	33.519516	4
TATCAAC	34520	0.0	33.46794	2
CAACGCA	35390	0.0	32.61789	5
AACGCAG	36735	0.0	31.459694	6
TGGTATC	4665	0.0	29.634003	2
TATAACG	435	0.0	28.104685	2
ACGCAGA	41280	0.0	27.847921	7
CGCAGAG	41395	0.0	27.668373	8
GCAGAGT	44255	0.0	25.699757	9
GTACATG	22465	0.0	25.0772	1
TACATGG	22760	0.0	24.213078	2
CATGGGT	9765	0.0	23.449934	4
ACATGGG	22795	0.0	23.412144	3
TACCTGG	3425	0.0	23.064463	2
GAGTACT	30855	0.0	22.817196	12-13
CAGAGTA	43540	0.0	22.764816	10-11
>>END_MODULE