FastQCFastQC Report
Wed 25 May 2016
SRR2431416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773900
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA238710.6325286838548981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55450.047.9413261
GTATCAA98800.039.4785081
TCAACGC116350.031.9840054
ATCAACG117050.031.8743863
TATCAAC124100.030.7468552
CAACGCA124400.029.9135075
AACGCAG130000.028.5584036
GTACATG215550.028.1800231
TACATGG215400.027.3688772
CATGGGT102050.025.9548284
ACATGGG220600.025.8699383
TACCTGG29450.025.5614972
CGCAGAG143050.025.3285858
ACGCAGA145700.025.1914527
GTACCTG32900.024.3117471
GCAGAGT157550.022.043289
ATGGGTG55300.021.1829015
CATGGGG89350.021.1141014
ATGGGTA44250.019.8810625
CAGAGTA153800.018.89864510-11
Produced by FastQC (version 0.11.5)