##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431416_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3773900
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.469938790111026	31.0	31.0	34.0	26.0	34.0
2	30.58286600068894	33.0	31.0	34.0	26.0	34.0
3	30.670493123824162	33.0	31.0	34.0	26.0	34.0
4	33.89254246270436	37.0	35.0	37.0	28.0	37.0
5	34.03426534884337	37.0	35.0	37.0	30.0	37.0
6	34.15815071941493	37.0	35.0	37.0	30.0	37.0
7	34.1109565701264	37.0	35.0	37.0	30.0	37.0
8	34.064147963645034	37.0	35.0	37.0	30.0	37.0
9	35.49350936696786	39.0	37.0	39.0	28.0	39.0
10-11	35.515584143724	39.0	37.0	39.0	29.5	39.0
12-13	35.44494236731233	39.0	37.0	39.0	28.5	39.0
14-15	36.77659278200271	40.5	38.0	41.0	28.5	41.0
16-17	36.80957471051167	40.0	38.0	41.0	29.0	41.0
18-19	36.84494647446938	40.0	38.0	41.0	29.0	41.0
20-21	36.94598929489388	40.5	38.0	41.0	29.0	41.0
22-23	36.932448793025785	40.0	38.0	41.0	29.0	41.0
24-25	36.99229033625693	40.0	38.0	41.0	30.0	41.0
26-27	37.0500666419354	40.0	38.0	41.0	30.0	41.0
28-29	37.02500635946899	40.0	38.0	41.0	30.0	41.0
30-31	36.962970534460375	40.0	38.0	41.0	30.0	41.0
32-33	36.912546437372484	40.0	38.0	41.0	30.0	41.0
34-35	36.84674037467872	40.0	38.0	41.0	30.0	41.0
36-37	36.71617888656297	40.0	37.0	41.0	30.0	41.0
38-39	36.596786480828854	40.0	37.0	41.0	29.0	41.0
40-41	36.53898937438724	40.0	37.0	41.0	29.0	41.0
42-43	36.37936498052413	40.0	37.0	41.0	28.5	41.0
44-45	36.23107223296854	40.0	36.0	41.0	28.0	41.0
46-47	36.10466957259069	40.0	36.0	41.0	28.0	41.0
48-49	35.97123188213784	40.0	36.0	41.0	28.0	41.0
50-51	35.84176952224489	40.0	35.0	41.0	27.5	41.0
52-53	35.684596571186304	40.0	35.0	41.0	27.0	41.0
54-55	35.52974999337555	40.0	35.0	41.0	27.0	41.0
56-57	35.3431256790058	39.0	35.0	41.0	27.0	41.0
58-59	35.128311560984656	39.0	35.0	41.0	26.0	41.0
60-61	34.89588568854501	39.0	34.0	41.0	26.0	41.0
62-63	34.80546424123585	39.0	34.0	41.0	26.0	41.0
64-65	34.5640925832693	38.0	34.0	40.5	26.0	41.0
66-67	34.3112271125361	38.0	34.0	40.0	25.5	41.0
68-69	34.02852963777524	37.0	34.0	40.0	25.0	41.0
70-71	33.711199952303986	37.0	33.5	40.0	24.5	41.0
72-73	33.38185603752086	36.0	33.0	39.0	24.0	41.0
74-75	33.10436630541349	36.0	33.0	39.0	23.5	41.0
76-77	32.838439412809024	35.5	33.0	39.0	23.0	40.5
78-79	32.48855573809587	35.0	33.0	37.5	22.0	39.5
80-81	32.156207504173395	35.0	33.0	37.0	22.0	39.0
82-83	31.816230027292722	35.0	32.0	37.0	20.5	39.0
84-85	31.504286017117572	35.0	32.0	36.0	20.0	38.0
86-87	31.212020721269774	35.0	32.0	36.0	18.5	37.0
88-89	30.938253530830174	35.0	32.0	35.5	17.5	37.0
90-91	30.6990501868094	35.0	32.0	35.0	13.5	36.5
92-93	30.45742309017197	35.0	31.5	35.0	7.0	36.0
94-95	30.245083600519358	35.0	31.0	35.0	2.0	36.0
96-97	30.045134741249107	35.0	31.0	35.0	2.0	36.0
98-99	29.832904422480723	35.0	31.0	35.0	2.0	35.5
100	29.662986035666023	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18599096977285612
1101	2	0.18609804845227274
1101	3	0.11635961468856593
1101	4	0.2598622308393459
1101	5	0.19434766265736414
1101	6	0.17524343125034392
1101	7	0.22427580869869246
1101	8	0.140808687323279
1101	9	0.22774415326980346
1101	10-11	0.17462242438925557
1101	12-13	0.115464474789853
1101	14-15	0.22217996412687313
1101	16-17	0.13980826900355936
1101	18-19	0.056875502841258196
1101	20-21	0.09233415388854382
1101	22-23	0.11353195582987041
1101	24-25	0.11988999944573919
1101	26-27	0.04478702734616036
1101	28-29	0.1209927279921601
1101	30-31	0.11694357237830388
1101	32-33	0.10283280579044174
1101	34-35	0.15681359826061936
1101	36-37	0.10519097436285563
1101	38-39	0.14437548886601093
1101	40-41	0.052395321212244994
1101	42-43	0.06266923186896634
1101	44-45	0.12342177218047112
1101	46-47	0.08625662871721573
1101	48-49	0.10549704428949269
1101	50-51	0.07312312093687012
1101	52-53	0.07113298018954595
1101	54-55	0.09849748754666621
1101	56-57	-0.0030884429192283847
1101	58-59	0.0367676341309604
1101	60-61	-0.03070172336107646
1101	62-63	0.02432864230361531
1101	64-65	0.055241663220606085
1101	66-67	0.026055120522293862
1101	68-69	0.06255665223891782
1101	70-71	0.038145650842750456
1101	72-73	-0.01813435643281025
1101	74-75	0.009790392052558161
1101	76-77	0.10126400131242264
1101	78-79	0.026294388181526074
1101	80-81	-0.008392815921972385
1101	82-83	-0.02310803620989077
1101	84-85	-0.07163216915786563
1101	86-87	-0.04632395220129837
1101	88-89	-0.041605151325075695
1101	90-91	-0.05096570031183845
1101	92-93	0.02230009132582822
1101	94-95	-0.0032544515785950523
1101	96-97	0.03809781527380096
1101	98-99	0.021443431867272267
1101	100	0.002510131037340102
1102	1	-0.1290174913977289
1102	2	-0.09442654412396934
1102	3	-0.0924446011816471
1102	4	0.02595225033682169
1102	5	0.030713222014313146
1102	6	0.06964986173998966
1102	7	0.04042009005100766
1102	8	-0.04275181004640416
1102	9	5.332126083601452E-4
1102	10-11	-9.647992431638386E-4
1102	12-13	-0.06916608475103914
1102	14-15	-0.05629049535147601
1102	16-17	-0.07082521515933848
1102	18-19	-0.06453861627730362
1102	20-21	-0.0733489016976705
1102	22-23	-0.03438861952972161
1102	24-25	-0.028253601334156997
1102	26-27	-0.05902233880180319
1102	28-29	-0.07525768586944537
1102	30-31	-0.053405083031321965
1102	32-33	-0.02406819851371722
1102	34-35	-0.03681937087450393
1102	36-37	-0.06726929492310063
1102	38-39	0.03260618452138431
1102	40-41	-0.045241069178807436
1102	42-43	0.012459541989265688
1102	44-45	-0.04399720291425524
1102	46-47	-0.08961937834602907
1102	48-49	-0.06407217176465707
1102	50-51	-0.10695634049123015
1102	52-53	-0.16404203728460942
1102	54-55	-0.17437434411282737
1102	56-57	-0.15285024257342172
1102	58-59	-0.2380442887620262
1102	60-61	-0.22060166082199828
1102	62-63	-0.1681864406526472
1102	64-65	-0.07742259385182848
1102	66-67	-0.14391784051954204
1102	68-69	-0.14170152382700962
1102	70-71	-0.18790003947840717
1102	72-73	-0.20237266714170232
1102	74-75	-0.10391348537083189
1102	76-77	-0.09246645522291885
1102	78-79	-0.1468933578362055
1102	80-81	-0.2323829936065529
1102	82-83	-0.15371678064574823
1102	84-85	-0.11381800221528948
1102	86-87	-0.17466427519729066
1102	88-89	-0.12570872708933578
1102	90-91	-0.19285263332144353
1102	92-93	-0.1533179420038273
1102	94-95	-0.15364302164076804
1102	96-97	-0.19347292746982703
1102	98-99	-0.2710486587776124
1102	100	-0.28139157223308686
1103	1	-0.38043260855927485
1103	2	-0.382856718362774
1103	3	-0.32549673949027635
1103	4	-0.0952931849732721
1103	5	-0.1548665632237416
1103	6	-0.11960790603874472
1103	7	-0.12892575634137415
1103	8	-0.17244012843460865
1103	9	-0.12365989705165248
1103	10-11	-0.11526604796117113
1103	12-13	-0.07180078494564412
1103	14-15	-0.1405561407540432
1103	16-17	-0.1176559378079105
1103	18-19	-0.20318549901111993
1103	20-21	-0.20634132843336772
1103	22-23	-0.21036940549745964
1103	24-25	-0.12470073109989954
1103	26-27	-0.09724266810644622
1103	28-29	-0.09292579950975721
1103	30-31	-0.1310125821466528
1103	32-33	-0.15651607623881603
1103	34-35	-0.14588676766652497
1103	36-37	-0.18011203696066502
1103	38-39	-0.17923811335040796
1103	40-41	-0.0866843185010282
1103	42-43	-0.15970767798167174
1103	44-45	-0.3077054779495185
1103	46-47	-0.38210274546270995
1103	48-49	-0.3305511875525369
1103	50-51	-0.4403726554004024
1103	52-53	-0.5093709551542247
1103	54-55	-0.39945828208649203
1103	56-57	-0.33976842217705894
1103	58-59	-0.38097059465292915
1103	60-61	-0.4118142776220779
1103	62-63	-0.331966368168942
1103	64-65	-0.2710585187982346
1103	66-67	-0.3398590709984788
1103	68-69	-0.3286626710093117
1103	70-71	-0.35276558400913416
1103	72-73	-0.36510305353205297
1103	74-75	-0.39166190970541237
1103	76-77	-0.40537471754371524
1103	78-79	-0.32311018419318316
1103	80-81	-0.35599987357806384
1103	82-83	-0.3117204872499002
1103	84-85	-0.3385739168325941
1103	86-87	-0.3250019258229173
1103	88-89	-0.37530862227938755
1103	90-91	-0.3718980062406416
1103	92-93	-0.4211746629207518
1103	94-95	-0.46824920655296864
1103	96-97	-0.39779694970776447
1103	98-99	-0.49361196106177374
1103	100	-0.44604474018835916
1104	1	-0.21950733994260574
1104	2	-0.09324451299886505
1104	3	-0.17007591673516487
1104	4	-0.0719097579900776
1104	5	-0.02265253619964369
1104	6	0.006831434120805113
1104	7	0.04450423487519117
1104	8	-0.003285864859392973
1104	9	0.0710695611428136
1104	10-11	-5.750924919851741E-4
1104	12-13	-0.04753696908492344
1104	14-15	-0.10905934730913458
1104	16-17	0.019971331747264287
1104	18-19	-0.06532480668398222
1104	20-21	-0.038162988622758576
1104	22-23	0.05404396938673983
1104	24-25	0.038440850640178326
1104	26-27	0.041638948045445545
1104	28-29	0.09132706713846517
1104	30-31	0.06956519937443773
1104	32-33	0.07705384831990614
1104	34-35	0.06064401646554529
1104	36-37	-0.036200160073128984
1104	38-39	0.08494845641773452
1104	40-41	0.029329205581220208
1104	42-43	0.0226313625785437
1104	44-45	0.007708005223591385
1104	46-47	9.422185011445094E-4
1104	48-49	-0.06264874383268904
1104	50-51	-0.11732396148504165
1104	52-53	-0.20927830797154456
1104	54-55	-0.05900936437403459
1104	56-57	-0.0764491262956426
1104	58-59	-0.19716195319752927
1104	60-61	-0.23672951146597399
1104	62-63	-0.12710347396721033
1104	64-65	-0.16295850796934275
1104	66-67	-0.1667100293553716
1104	68-69	-0.11201541613331045
1104	70-71	-0.19760477497062112
1104	72-73	-0.23140270099147386
1104	74-75	-0.18433857103784845
1104	76-77	-0.14654243300856962
1104	78-79	-0.21822286482510833
1104	80-81	-0.1474628568173486
1104	82-83	-0.19837472074486584
1104	84-85	-0.2391146394806185
1104	86-87	-0.16321780579066925
1104	88-89	-0.2086196160106617
1104	90-91	-0.29112530467286035
1104	92-93	-0.33221664292340236
1104	94-95	-0.30356077782512614
1104	96-97	-0.31567484794024736
1104	98-99	-0.36156674442378645
1104	100	-0.34805073217497906
1105	1	-0.06957338851324124
1105	2	-0.04394421267068793
1105	3	-0.037513439782966174
1105	4	0.11815296578985368
1105	5	-0.003170849129958242
1105	6	0.11078599418939206
1105	7	0.13262850441276441
1105	8	0.09609469456617603
1105	9	0.11767424943524674
1105	10-11	0.05969360797897849
1105	12-13	0.16377729367649607
1105	14-15	0.06937587454470417
1105	16-17	-0.0522726403818794
1105	18-19	0.07848287189482761
1105	20-21	0.07344893573065292
1105	22-23	0.04272050860028287
1105	24-25	-0.01577316968154463
1105	26-27	0.04760941141494612
1105	28-29	0.06375115178729374
1105	30-31	0.04956604322995162
1105	32-33	0.05025623778116994
1105	34-35	0.10940986934608787
1105	36-37	0.05141126471783508
1105	38-39	0.062131430451458414
1105	40-41	0.02157965852066468
1105	42-43	0.01553710307440781
1105	44-45	0.015764833828939118
1105	46-47	-0.012345552260434545
1105	48-49	-0.13431349354092958
1105	50-51	-0.06590092574860051
1105	52-53	0.006508625263450085
1105	54-55	-0.015141198232385022
1105	56-57	-0.07599990825435299
1105	58-59	-0.05546033544213458
1105	60-61	-0.05766188741710465
1105	62-63	0.09742105986591554
1105	64-65	-0.019629734268598042
1105	66-67	-0.0020829164466604766
1105	68-69	0.0352404585777748
1105	70-71	0.0215698668338149
1105	72-73	0.04003123735813574
1105	74-75	0.049401219365321936
1105	76-77	0.06286282606772886
1105	78-79	0.07883479666563176
1105	80-81	0.06907785878900086
1105	82-83	0.026872879119103743
1105	84-85	0.030150607089741044
1105	86-87	0.013632216613693515
1105	88-89	0.0014320701392200874
1105	90-91	-0.04104256464027145
1105	92-93	-0.040617207631509444
1105	94-95	-9.543106852802907E-4
1105	96-97	0.04224899747555
1105	98-99	0.02630323769491838
1105	100	0.009360926701496908
1106	1	-0.21760758914901857
1106	2	-0.2513410616924112
1106	3	-0.20401146999074626
1106	4	-0.1806428527174262
1106	5	-0.09643128022244696
1106	6	-0.09443541333133254
1106	7	-0.061000624938976955
1106	8	-0.07099784931723718
1106	9	-0.1029149082672447
1106	10-11	-0.055106890883408255
1106	12-13	-0.095678675599558
1106	14-15	-0.0881677549900104
1106	16-17	-0.08917679862643269
1106	18-19	-0.14356266871267565
1106	20-21	-0.16983752851601963
1106	22-23	-0.06216046082101201
1106	24-25	-0.05497824978827026
1106	26-27	-0.0904032146730458
1106	28-29	-0.10586667205632949
1106	30-31	-0.06313565463454296
1106	32-33	-0.13865899188154174
1106	34-35	-0.06213391228801868
1106	36-37	-0.08238683938321145
1106	38-39	-0.15799622669693747
1106	40-41	-0.14598259153815718
1106	42-43	-0.09254374684677913
1106	44-45	-0.14518805083918096
1106	46-47	-0.11680058429042361
1106	48-49	-0.14574121618003488
1106	50-51	-0.1589957838141629
1106	52-53	-0.22166346197648323
1106	54-55	-0.2394336513025479
1106	56-57	-0.25927365838313676
1106	58-59	-0.25550967420198845
1106	60-61	-0.24240158820535385
1106	62-63	-0.28938969707343887
1106	64-65	-0.22600426134172125
1106	66-67	-0.12636322191502813
1106	68-69	-0.1049536811709828
1106	70-71	-0.179951478334047
1106	72-73	-0.16629090502850374
1106	74-75	-0.16827256313955985
1106	76-77	-0.1304425422893445
1106	78-79	-0.10669495775423599
1106	80-81	-0.06304190035040591
1106	82-83	-0.11814887381889605
1106	84-85	-0.18253113798595777
1106	86-87	-0.19662279801380933
1106	88-89	-0.1764393779057869
1106	90-91	-0.1972560137258199
1106	92-93	-0.12547222693949678
1106	94-95	-0.20664365196721235
1106	96-97	-0.1268128134676587
1106	98-99	-0.1465723819238569
1106	100	-0.15532871585802965
1107	1	-0.4267179430295833
1107	2	-0.43084032961932195
1107	3	-0.3673847968450481
1107	4	-0.39477912309580887
1107	5	-0.3256043155839663
1107	6	-0.35415275958302317
1107	7	-0.32585643599045966
1107	8	-0.33529177800616594
1107	9	-0.30357930281800094
1107	10-11	-0.3047721177570253
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2216	58-59	0.19952233547046205
2216	60-61	0.2711809940349639
2216	62-63	0.23385021828860886
2216	64-65	0.13461030932209184
2216	66-67	0.0813367645730736
2216	68-69	0.11400741361593703
2216	70-71	0.09783739132008407
2216	72-73	0.09843595640806768
2216	74-75	0.005814433241091876
2216	76-77	0.14187877508090452
2216	78-79	0.1516772582892969
2216	80-81	0.08760613691192276
2216	82-83	0.10474658222896238
2216	84-85	0.16087579366773852
2216	86-87	0.07496624370602589
2216	88-89	0.12072195718751644
2216	90-91	0.16948239951799593
2216	92-93	0.14321068951946714
2216	94-95	0.12347010010341819
2216	96-97	0.1282228207277889
2216	98-99	0.14847777018919217
2216	100	0.155204402166099
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91746.0
3	17959.0
4	12909.0
5	11355.0
6	7299.0
7	6155.0
8	6914.0
9	7439.0
10	7728.0
11	8565.0
12	9821.0
13	11262.0
14	11833.0
15	11944.0
16	11781.0
17	11325.0
18	11631.0
19	10848.0
20	10686.0
21	11336.0
22	13597.0
23	21148.0
24	33701.0
25	44374.0
26	48673.0
27	37930.0
28	34846.0
29	42989.0
30	51115.0
31	56106.0
32	71532.0
33	95231.0
34	133189.0
35	207818.0
36	345924.0
37	647323.0
38	1044322.0
39	551224.0
40	2322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.883242540734564	22.05968155608657	14.440827841624296	21.61624806155457
2	14.444524370035195	22.637563960011605	41.0729913231776	21.84492034677561
3	16.93210618713493	29.690538392740763	30.79946173203037	22.57789368809394
4	11.131718375061052	19.85249304538022	39.837468943110146	29.17831963644858
5	11.564991018859068	41.187437977562816	34.81897810916586	12.428592894412258
6	26.755967604941727	41.15570412527588	19.34382531849722	12.744502951285167
7	24.24938080413257	36.13757020646934	23.38131327863549	16.231735710762603
8	23.75627543117323	37.88997559031609	21.16886484629005	17.184884132220624
9	24.923999183385607	18.894972253670126	21.641951708394423	34.53907685454985
10-11	21.826585981109904	29.9390270904382	29.473536553383912	18.76085037506798
12-13	22.74026707537614	28.237122204485548	28.951002593325235	20.07160812681307
14-15	20.700323310178636	29.630477652141796	26.585931486243076	23.083267551436492
16-17	19.627208507736306	32.67965693722129	27.256579585823765	20.436554969218637
18-19	19.777341469717065	31.47150822775387	29.196358646993648	19.554791655535418
20-21	21.81260802212972	30.271692063412935	28.343773815250138	19.571926099207207
22-23	21.267797464977818	30.38506600595526	28.47505567394274	19.87208085512418
24-25	20.705466427475468	30.516671812963125	28.49202288694371	20.285838872617695
26-27	20.407561491010327	30.50066014153201	28.554972858491055	20.5368055089666
28-29	20.346342902410004	30.537452643511294	28.72018322776524	20.39602122631347
30-31	20.60611090920879	30.88506183448197	28.336306181088126	20.172521075221113
32-33	20.24171211110155	30.642526922272744	28.829845190385345	20.28591577624036
34-35	20.520502550712287	30.381060253608833	28.69743237002849	20.401004825650393
36-37	20.63868243653134	30.552603394763178	28.40687937517412	20.40183479353136
38-39	20.553551497112537	30.580032824541448	28.540525102500453	20.325890575845563
40-41	20.390550441126674	30.35492725142192	28.76971652331033	20.484805784141077
42-43	20.34488149074213	30.313406042824585	28.810489535431195	20.53122293100209
44-45	20.559880784227193	30.433650290852327	28.644127445671057	20.36234147924942
46-47	20.586370821024957	30.54692346847653	28.50776940873787	20.358936301760643
48-49	20.496938389489046	30.338658052070784	28.551654492225076	20.61274906621509
50-51	20.4542920200561	30.311230209583996	28.581335203786338	20.653142566573564
52-53	20.471013027520577	30.334699601177782	28.593891479360067	20.600395891941574
54-55	20.590967137303124	30.587025093436253	28.40908895030386	20.41291881895676
56-57	20.56998201329632	30.35425294189868	28.301109034215045	20.774656010589958
58-59	20.528429439117694	30.10028101065183	28.620987264554298	20.750302285676174
60-61	20.67225141441825	30.446965448019547	28.24744728499318	20.633335852569022
62-63	20.52227116082782	30.36491470995844	28.464975471845953	20.647838657367785
64-65	20.590831233295102	30.163001504973998	28.541617748271985	20.704549513458918
66-67	20.369183242567775	30.25441094092339	28.358271487903263	21.01813432860557
68-69	20.782499366584876	30.35253678282393	28.24615141478621	20.618812435804983
70-71	20.68190532150882	30.154235565075222	28.32690419990168	20.83695491351428
72-73	20.51850487812671	30.303208675324544	28.511550576252787	20.66673587029596
74-75	20.538501802306804	30.350467401236397	28.379955017901093	20.731075778555706
76-77	20.585541037367545	30.275542094090213	28.23476797910208	20.904148889440158
78-79	20.65703554414808	30.282297876955454	28.390200337477754	20.670466241418705
80-81	20.506526024807858	30.251369566220497	28.440410278495705	20.801694130475944
82-83	20.41107578886653	30.279109083857364	28.620061193302547	20.689753933973552
84-85	20.444111180946166	30.157354294458187	28.46806637335943	20.930468151236212
86-87	20.634030578068373	30.29382875030032	28.296593579634568	20.775547091996742
88-89	20.507126367892692	30.12156394239503	28.421697466489693	20.949612223222587
90-91	20.449387548363656	30.04530736612648	28.418922753393744	21.08638233211612
92-93	20.53708473467076	30.152600657315475	28.381303963395677	20.92901064461809
94-95	20.400523325588807	30.112421133911	28.4774794458943	21.009576094605897
96-97	20.39215187147966	30.183870575987974	28.37740839086001	21.04656916167236
98-99	20.421567931984814	30.168851255892953	28.33239081418406	21.07718999793817
100	20.468303084228516	30.238912013493746	28.200475516723312	21.092309385554422
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20983.0
1	15304.0
2	8448.0
3	6143.0
4	4184.0
5	2997.0
6	2500.5
7	2583.5
8	3027.5
9	3063.0
10	2386.0
11	1776.0
12	1622.0
13	1648.0
14	1742.0
15	1891.0
16	2105.5
17	2501.5
18	3290.5
19	4292.5
20	5994.0
21	8775.5
22	12798.0
23	18856.0
24	25858.5
25	32745.5
26	41996.0
27	53871.5
28	68195.5
29	81929.5
30	91238.0
31	100510.5
32	110494.0
33	119332.0
34	126849.0
35	132066.5
36	133919.0
37	134475.0
38	134045.0
39	132746.5
40	132193.5
41	128502.0
42	122416.0
43	117969.5
44	114255.0
45	110847.0
46	109079.5
47	106419.5
48	102568.5
49	100468.0
50	98860.5
51	94512.5
52	88441.5
53	83112.5
54	79972.5
55	77503.0
56	71086.5
57	63229.0
58	57288.5
59	51394.5
60	44135.0
61	36376.5
62	30050.0
63	24316.0
64	18704.5
65	14524.0
66	11297.5
67	8473.5
68	6377.0
69	4809.5
70	3643.5
71	2837.0
72	2170.0
73	1578.5
74	1261.0
75	1001.5
76	688.0
77	539.0
78	426.0
79	311.5
80	231.5
81	177.0
82	154.0
83	143.5
84	126.0
85	102.5
86	89.0
87	77.5
88	63.0
89	59.0
90	53.5
91	44.0
92	38.0
93	36.5
94	30.5
95	21.5
96	17.0
97	18.5
98	21.0
99	19.5
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17769416253742812
2	0.17242110283791304
3	0.18556400540554865
4	0.17541535281803972
5	0.1886112509605448
6	0.07501523622777498
7	0.07631362781207769
8	0.06481358806539654
9	0.05909006597949071
10-11	0.07199448846021357
12-13	0.07101407032512785
14-15	0.06886774954291316
16-17	0.06523755266435254
18-19	0.06914597631097803
20-21	0.07106706589999734
22-23	0.06919897188584753
24-25	0.06835104268793556
26-27	0.07257743978377805
28-29	0.06988791435915101
30-31	0.07484300060944911
32-33	0.07206073292880044
34-35	0.0715042793926707
36-37	0.08271284347756963
38-39	0.07792999284559739
40-41	0.07316039110734254
42-43	0.07592940989427382
44-45	0.08632979146241289
46-47	0.07749277935292402
48-49	0.0818914120670924
50-51	0.07889716208696575
52-53	0.07741328599061979
54-55	0.07985108243461671
56-57	0.07369034685603752
58-59	0.08072550941996343
60-61	0.07724105037229392
62-63	0.06946394976019503
64-65	0.07256419089006068
66-67	0.07253769310262594
68-69	0.07092132806910623
70-71	0.06693341106017647
72-73	0.06416439227324519
74-75	0.0694771986539124
76-77	0.07436604043562363
78-79	0.07123930151832322
80-81	0.0794403667293781
82-83	0.08114947401891942
84-85	0.08240811892207
86-87	0.07848644638172712
88-89	0.08068576273881131
90-91	0.0792283844299001
92-93	0.07025888338323749
94-95	0.06979517210312938
96-97	0.06905323405495642
98-99	0.07889716208696575
100	0.08651527597445613
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3773900.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.23930631542098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46682837245636	47.72469876099071
2	15.223146624511063	19.254024649440442
3	4.75042310925624	9.0124038640233
4	1.8743571353295876	4.74132180102411
5	0.9102241079371958	2.878097058876057
6	0.5009567585521373	1.9008094742915407
7	0.3118408885267264	1.3804421029850293
8	0.19326866562993547	0.9777741077555333
9	0.13549878176202096	0.7711964068693339
>10	0.5819032658627714	6.358745731171172
>50	0.031147123640200967	1.3595779582476044
>100	0.01910600080797954	2.2713816322288647
>500	8.381714667914439E-4	0.3647300253397351
>1k	4.190856918320926E-4	0.37215075104891704
>5k	0.0	0.0
>10k+	4.190856918320755E-5	0.6326456757077649
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23871	0.6325286838548981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.6497787434749198E-5	0.0	0.0	0.0	0.0
6	2.6497787434749198E-5	0.0	0.0	0.0	0.0
7	2.6497787434749198E-5	0.0	0.0	0.0	0.0
8	2.6497787434749198E-5	0.0	0.0	0.0	0.0
9	2.6497787434749198E-5	0.0	0.0	5.2995574869498396E-5	0.0
10-11	2.6497787434749198E-5	0.0	0.0	5.2995574869498396E-5	0.0
12-13	2.6497787434749198E-5	0.0	0.0	5.2995574869498396E-5	0.0
14-15	2.6497787434749198E-5	0.0	0.0	5.2995574869498396E-5	0.0
16-17	2.6497787434749198E-5	0.0	0.0	6.6244468586873E-5	0.0
18-19	2.6497787434749198E-5	0.0	0.0	7.949336230424759E-5	0.0
20-21	2.6497787434749198E-5	0.0	0.0	1.5898672460849518E-4	0.0
22-23	2.6497787434749198E-5	0.0	0.0	2.782267680648666E-4	0.0
24-25	5.2995574869498396E-5	0.0	0.0	5.697024298471078E-4	0.0
26-27	5.2995574869498396E-5	0.0	0.0	8.081825167598506E-4	0.0
28-29	5.2995574869498396E-5	0.0	0.0	0.0015633694586502027	0.0
30-31	5.2995574869498396E-5	0.0	0.0	0.004107157052386126	0.0
32-33	5.2995574869498396E-5	0.0	0.0	0.00855878534142399	0.0
34-35	5.2995574869498396E-5	0.0	0.0	0.0148652587508943	0.0
36-37	5.2995574869498396E-5	0.0	0.0	0.02586184053631522	0.0
38-39	5.2995574869498396E-5	0.0	0.0	0.0421447309149686	0.0
40-41	5.2995574869498396E-5	0.0	0.0	0.06302498741355098	0.0
42-43	6.6244468586873E-5	0.0	0.0	0.0820503987917009	0.0
44-45	7.949336230424759E-5	0.0	0.0	0.10617663425104004	0.0
46-47	7.949336230424759E-5	0.0	0.0	0.13532420042926416	0.0
48-49	7.949336230424759E-5	0.0	0.0	0.1712419512970667	0.0
50-51	7.949336230424759E-5	0.0	0.0	0.20808712472508545	0.0
52-53	9.274225602162218E-5	0.0	0.0	0.24441559129812662	0.0
54-55	1.0599114973899679E-4	0.0	0.0	0.28400328572564193	0.0
56-57	1.0599114973899679E-4	0.0	0.0	0.3333421659291449	0.0
58-59	1.0599114973899679E-4	0.0	0.0	0.392445480802353	0.0
60-61	1.0599114973899679E-4	0.0	0.0	0.4566628686504677	0.0
62-63	1.0599114973899679E-4	0.0	0.0	0.5250271602321206	0.0
64-65	1.0599114973899679E-4	0.0	0.0	0.5922520469540793	0.0
66-67	1.0599114973899679E-4	0.0	0.0	0.6730967964174992	0.0
68-69	1.0599114973899679E-4	1.3248893717374599E-5	0.0	0.7719202946553962	1.3248893717374599E-5
70-71	1.0599114973899679E-4	2.6497787434749198E-5	0.0	0.8818993614033228	2.6497787434749198E-5
72-73	1.0599114973899679E-4	2.6497787434749198E-5	0.0	0.9998012665942393	2.6497787434749198E-5
74-75	1.0599114973899679E-4	2.6497787434749198E-5	0.0	1.1168817403746787	2.6497787434749198E-5
76-77	1.0599114973899679E-4	2.6497787434749198E-5	0.0	1.2459392140756247	2.6497787434749198E-5
78-79	1.0599114973899679E-4	2.6497787434749198E-5	0.0	1.4003285725641907	2.6497787434749198E-5
80-81	1.0599114973899679E-4	2.6497787434749198E-5	0.0	1.5736108534937332	2.6497787434749198E-5
82-83	1.0599114973899679E-4	2.6497787434749198E-5	0.0	1.7638649672752327	2.6497787434749198E-5
84-85	1.0599114973899679E-4	2.6497787434749198E-5	0.0	1.9498529372797373	2.6497787434749198E-5
86-87	1.0599114973899679E-4	2.6497787434749198E-5	0.0	2.1483213651660087	2.6497787434749198E-5
88	1.0599114973899679E-4	2.6497787434749198E-5	0.0	2.3142372611886906	2.6497787434749198E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5545	0.0	47.941326	1
GTATCAA	9880	0.0	39.478508	1
TCAACGC	11635	0.0	31.984005	4
ATCAACG	11705	0.0	31.874386	3
TATCAAC	12410	0.0	30.746855	2
CAACGCA	12440	0.0	29.913507	5
AACGCAG	13000	0.0	28.558403	6
GTACATG	21555	0.0	28.180023	1
TACATGG	21540	0.0	27.368877	2
CATGGGT	10205	0.0	25.954828	4
ACATGGG	22060	0.0	25.869938	3
TACCTGG	2945	0.0	25.561497	2
CGCAGAG	14305	0.0	25.328585	8
ACGCAGA	14570	0.0	25.191452	7
GTACCTG	3290	0.0	24.311747	1
GCAGAGT	15755	0.0	22.04328	9
ATGGGTG	5530	0.0	21.182901	5
CATGGGG	8935	0.0	21.114101	4
ATGGGTA	4425	0.0	19.881062	5
CAGAGTA	15380	0.0	18.898645	10-11
>>END_MODULE