FastQCFastQC Report
Wed 25 May 2016
SRR2431416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773900
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323450.8570709345769627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258370.684623333951615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178520.4730385012851427No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56390.14942102334455074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55180.1462147910649461No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50620.13413179999470043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47580.1260764726145367No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40640.10768700813482074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116400.064.888121
GTATCAA229700.045.8422281
GTGGTAT37250.036.2349971
TGGTATC37700.035.1759452
ATCAACG305750.034.2646643
TATCAAC306450.034.2202642
TCAACGC308850.033.9359664
CAACGCA320250.032.771995
AACGCAG329200.032.007736
TACCTGG26800.029.8299272
ACGCAGA367350.028.5429337
CGCAGAG367650.028.4940788
GCAGAGT393200.026.4752039
GTACATG207400.026.4627341
TACATGG208950.025.7692152
CATGGGT97300.025.7584
GTACCTG32650.025.2073651
ACATGGG207250.025.0025793
GAGTACT274300.023.26767512-13
AGAGTAC378150.022.7874610-11
Produced by FastQC (version 0.11.5)