##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431416_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3773900
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.646348869869364	34.0	31.0	34.0	31.0	34.0
2	32.74262540077903	34.0	31.0	34.0	31.0	34.0
3	32.83910914438644	34.0	31.0	34.0	31.0	34.0
4	36.31373247833806	37.0	37.0	37.0	35.0	37.0
5	36.22650202708074	37.0	37.0	37.0	35.0	37.0
6	36.27271814303506	37.0	37.0	37.0	35.0	37.0
7	36.22414266408755	37.0	37.0	37.0	35.0	37.0
8	36.23630090887411	37.0	37.0	37.0	35.0	37.0
9	38.02671162457935	39.0	39.0	39.0	35.0	39.0
10-11	38.00129984896262	39.0	39.0	39.0	35.0	39.0
12-13	37.98188452264236	39.0	39.0	39.0	35.0	39.0
14-15	39.42299067277882	41.0	39.0	41.0	36.0	41.0
16-17	39.46062071067066	41.0	39.0	41.0	36.0	41.0
18-19	39.54997694692493	41.0	39.5	41.0	36.5	41.0
20-21	39.552317496489046	41.0	40.0	41.0	37.0	41.0
22-23	39.48545682185538	41.0	39.5	41.0	36.0	41.0
24-25	39.43792668062217	41.0	39.0	41.0	36.0	41.0
26-27	39.32141484936008	41.0	39.0	41.0	36.0	41.0
28-29	39.23823021277723	41.0	39.0	41.0	35.0	41.0
30-31	39.04487758022205	41.0	39.0	41.0	35.0	41.0
32-33	38.80562018071491	41.0	38.0	41.0	35.0	41.0
34-35	38.66626858157344	41.0	38.0	41.0	35.0	41.0
36-37	38.521161133045396	41.0	38.0	41.0	35.0	41.0
38-39	38.374378494395714	41.0	38.0	41.0	35.0	41.0
40-41	38.23083838999443	40.0	38.0	41.0	34.5	41.0
42-43	38.04870889530724	40.0	38.0	41.0	34.0	41.0
44-45	37.78042051988659	40.0	38.0	41.0	33.0	41.0
46-47	37.37378732875805	40.0	38.0	41.0	33.0	41.0
48-49	37.0135946898434	40.0	37.0	41.0	31.5	41.0
50-51	36.83922652958478	40.0	37.0	41.0	31.0	41.0
52-53	36.7825725376931	40.0	37.0	41.0	31.0	41.0
54-55	36.64835832957948	40.0	37.0	41.0	31.0	41.0
56-57	36.50737976628952	40.0	36.0	41.0	31.0	41.0
58-59	36.33903495058163	40.0	36.0	41.0	30.5	41.0
60-61	36.19364477066165	40.0	35.0	41.0	30.0	41.0
62-63	36.04222210445428	39.0	35.0	41.0	30.0	41.0
64-65	35.81841635973396	39.0	35.0	41.0	30.0	41.0
66-67	35.56384496144572	39.0	35.0	41.0	29.0	41.0
68-69	35.27823299504492	38.0	35.0	41.0	29.0	41.0
70-71	34.981268184106625	37.0	35.0	40.0	29.0	41.0
72-73	34.665777180105465	37.0	35.0	40.0	29.0	41.0
74-75	34.32658946977927	36.5	35.0	39.0	28.0	41.0
76-77	33.986538594027394	36.0	35.0	39.0	28.0	41.0
78-79	33.65918943268237	35.5	34.5	38.5	28.0	40.0
80-81	33.38436299319007	35.0	34.0	37.0	28.0	39.0
82-83	33.1697747688068	35.0	34.0	37.0	28.0	39.0
84-85	32.89423394896526	35.0	34.0	36.5	28.0	39.0
86-87	32.64272582739341	35.0	34.0	36.0	27.5	37.0
88-89	32.40052081401203	35.0	34.0	36.0	27.0	37.0
90-91	32.20864357826122	35.0	34.0	35.5	27.0	37.0
92-93	32.04129600678343	35.0	34.0	35.0	26.0	36.0
94-95	31.89998463128329	35.0	34.0	35.0	26.0	36.0
96-97	31.758443254988208	35.0	34.0	35.0	26.0	36.0
98-99	31.64197580752007	35.0	34.0	35.0	25.0	36.0
100	31.538873844034022	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.03058562727224512
1101	2	0.0029934639441790978
1101	3	-0.03223877603712566
1101	4	0.021846569934474758
1101	5	0.023315739800892743
1101	6	-0.022562061953998125
1101	7	-0.020541191838901796
1101	8	0.059052512877222796
1101	9	-0.006767166216356202
1101	10-11	0.06506512893814431
1101	12-13	0.04694508459324709
1101	14-15	0.06801319827206953
1101	16-17	0.004354801843980738
1101	18-19	-0.03128783892417175
1101	20-21	0.02302642898216334
1101	22-23	0.045425706795008125
1101	24-25	0.05756174680089998
1101	26-27	0.02769069930798196
1101	28-29	-0.012043395579048877
1101	30-31	-0.04242544638437806
1101	32-33	-0.032653989926323845
1101	34-35	-0.011511624566132639
1101	36-37	-0.02330369621083861
1101	38-39	-0.06762798129204839
1101	40-41	-0.036932780481166105
1101	42-43	-0.06835603865267359
1101	44-45	-0.09129321661724532
1101	46-47	-0.06444482050064693
1101	48-49	-0.025350817285200833
1101	50-51	-0.11038092237564712
1101	52-53	-0.05847085880008507
1101	54-55	-0.04719641223739046
1101	56-57	-0.012610298827361532
1101	58-59	-0.09792237101660106
1101	60-61	-0.08896624798358488
1101	62-63	-0.09400888829080145
1101	64-65	-0.08675578801930328
1101	66-67	-0.04918699213769884
1101	68-69	-0.06662880791540005
1101	70-71	-0.11785024112118236
1101	72-73	-0.17197003376295328
1101	74-75	-0.10545102981658516
1101	76-77	-0.09381659912091322
1101	78-79	-0.08375536773820613
1101	80-81	-0.07480604276904756
1101	82-83	-0.06224934985829833
1101	84-85	-0.05623796003846593
1101	86-87	-0.02765963123293602
1101	88-89	0.0035161314686860123
1101	90-91	-0.09785867788873048
1101	92-93	-0.08802482593539196
1101	94-95	-0.13112139929083
1101	96-97	-0.12073434514366
1101	98-99	-0.11685692059663566
1101	100	-0.10859137536666807
1102	1	-0.0352827463436185
1102	2	-0.03879184304916805
1102	3	-0.015093869293941964
1102	4	0.03538536256221647
1102	5	-0.055014466578100496
1102	6	-0.01569195931631384
1102	7	-0.02424755243191612
1102	8	0.0030064546790811164
1102	9	-0.006081995337495982
1102	10-11	-0.001049722255615393
1102	12-13	-0.016528869716431416
1102	14-15	0.003225923136874087
1102	16-17	-0.061798216586659294
1102	18-19	-0.08718398725217469
1102	20-21	-0.029111396129117395
1102	22-23	-0.00872946371597294
1102	24-25	-0.0138224823128823
1102	26-27	-0.029598323242879587
1102	28-29	-0.08926997101222156
1102	30-31	-0.05203209392144004
1102	32-33	-0.09204542942894989
1102	34-35	-0.08581265830044771
1102	36-37	-0.1191949701688273
1102	38-39	-0.07716887081858914
1102	40-41	-0.2235066501791323
1102	42-43	-0.19845435377609277
1102	44-45	-0.2535481185780242
1102	46-47	-0.27621964300000457
1102	48-49	-0.2892344945835106
1102	50-51	-0.2622318034410185
1102	52-53	-0.2911475317206609
1102	54-55	-0.20844149188275907
1102	56-57	-0.20953576695082887
1102	58-59	-0.2225945006564487
1102	60-61	-0.30215923624830765
1102	62-63	-0.23292889344108403
1102	64-65	-0.2888655625107148
1102	66-67	-0.26444423011732
1102	68-69	-0.23893962717743733
1102	70-71	-0.1879105729462225
1102	72-73	-0.24259689614017788
1102	74-75	-0.20522386578024054
1102	76-77	-0.25504650384077365
1102	78-79	-0.22233324729528192
1102	80-81	-0.21826381049152133
1102	82-83	-0.28078207251584075
1102	84-85	-0.24139688289466932
1102	86-87	-0.2537080806314549
1102	88-89	-0.2436185666882551
1102	90-91	-0.26188122874772546
1102	92-93	-0.32345045960349594
1102	94-95	-0.3088266746467525
1102	96-97	-0.33044859669794135
1102	98-99	-0.3500421834447387
1102	100	-0.30018170755958096
1103	1	-0.07249224753714145
1103	2	-0.14430994906642525
1103	3	-0.07807393177484556
1103	4	-0.024562004615219735
1103	5	-0.09549161345211132
1103	6	-0.011171713780868231
1103	7	-0.09207683317011828
1103	8	-0.0199321845243432
1103	9	-0.023138014370935878
1103	10-11	-0.015454901881184924
1103	12-13	-0.024208709075352886
1103	14-15	-0.1008301248697947
1103	16-17	-0.07060273759168467
1103	18-19	-0.1137474445290323
1103	20-21	-0.09883360616196057
1103	22-23	-0.07149644638773367
1103	24-25	-0.0565467772929793
1103	26-27	-0.05264276395549672
1103	28-29	-0.0836117527138569
1103	30-31	-0.12496126056701229
1103	32-33	-0.14429150861981554
1103	34-35	-0.18986093557480643
1103	36-37	-0.07643087216167999
1103	38-39	-0.07419588005570432
1103	40-41	-0.1388622431092159
1103	42-43	-0.10049583415615615
1103	44-45	-0.1412803052466316
1103	46-47	-0.0416984495055317
1103	48-49	-0.05392476514846578
1103	50-51	-0.19521331598980396
1103	52-53	-0.16162735650858195
1103	54-55	-0.11513314022315058
1103	56-57	-0.11451138443219833
1103	58-59	-0.14008264976224183
1103	60-61	-0.17209525042100182
1103	62-63	-0.17965707758479255
1103	64-65	-0.23526030839862244
1103	66-67	-0.231063871546894
1103	68-69	-0.16747399533321783
1103	70-71	-0.1455365235072037
1103	72-73	-0.20083156578630934
1103	74-75	-0.18108122017007844
1103	76-77	-0.12488831986021864
1103	78-79	-0.1247522235220373
1103	80-81	-0.09631925540497832
1103	82-83	-0.08741847481726239
1103	84-85	-0.10945681021968312
1103	86-87	-0.03961877951635273
1103	88-89	-0.05356160897846962
1103	90-91	-0.06001961444946602
1103	92-93	-0.08996639516028182
1103	94-95	-0.08411281340432097
1103	96-97	-0.07912831059996961
1103	98-99	-0.07363203111307115
1103	100	-0.031810008608861295
1104	1	-0.09594570135192271
1104	2	-0.03420057726245318
1104	3	-0.0791510455143083
1104	4	-0.03625287585970227
1104	5	-0.04873655094534257
1104	6	-0.09436431163193504
1104	7	-0.07925398180267251
1104	8	-0.13030241229202488
1104	9	-0.11558137325243223
1104	10-11	-0.08897633707027097
1104	12-13	-0.08135866515818435
1104	14-15	-0.07761237992628622
1104	16-17	-0.10841951274780115
1104	18-19	-0.0979099500938787
1104	20-21	-0.04834041031543279
1104	22-23	-0.046423231049210756
1104	24-25	-0.030927545181704374
1104	26-27	-0.08675954753408632
1104	28-29	-0.14817649143677158
1104	30-31	-0.08079767211301458
1104	32-33	-0.10030972522171311
1104	34-35	-0.13690825783059068
1104	36-37	-0.1328166694688946
1104	38-39	-0.16944319990506784
1104	40-41	-0.26807534366149355
1104	42-43	-0.2578531033503637
1104	44-45	-0.2872201928667977
1104	46-47	-0.3726114453785243
1104	48-49	-0.3547821727118361
1104	50-51	-0.40759735661017515
1104	52-53	-0.3729719438676611
1104	54-55	-0.3029094875177307
1104	56-57	-0.3379927527227977
1104	58-59	-0.2720181336332175
1104	60-61	-0.31995750692134095
1104	62-63	-0.39286736262528166
1104	64-65	-0.44714874675214844
1104	66-67	-0.4230450774041259
1104	68-69	-0.4227580130925972
1104	70-71	-0.43748512236896886
1104	72-73	-0.3920234657754733
1104	74-75	-0.3847843400940292
1104	76-77	-0.4532527962833157
1104	78-79	-0.46927027769056906
1104	80-81	-0.4386344724039333
1104	82-83	-0.4280149060959175
1104	84-85	-0.47232177394290176
1104	86-87	-0.503102433906264
1104	88-89	-0.422614802899858
1104	90-91	-0.42425936610688453
1104	92-93	-0.4164667380751794
1104	94-95	-0.4280009870516146
1104	96-97	-0.42241235242355657
1104	98-99	-0.4545854548475958
1104	100	-0.3781728246019327
1105	1	-0.11807743225990208
1105	2	-0.10900043583227159
1105	3	-0.1205903929777179
1105	4	-0.03444121934602151
1105	5	-0.057243559587270454
1105	6	-0.04132103956532518
1105	7	0.0041955307441909895
1105	8	0.02683205692707702
1105	9	0.028721732895306218
1105	10-11	-0.05601240429855636
1105	12-13	-0.004273156435665726
1105	14-15	-0.09648714923728363
1105	16-17	-0.06515412947285171
1105	18-19	-0.06967905097427263
1105	20-21	-0.028041196029299442
1105	22-23	-0.0661535179516406
1105	24-25	-0.03278536059168857
1105	26-27	0.0391269522123423
1105	28-29	0.05604344431207409
1105	30-31	0.05256933668080421
1105	32-33	0.022242954568028495
1105	34-35	-0.025462708029188263
1105	36-37	0.01259462148657775
1105	38-39	0.0041941633165052394
1105	40-41	-0.02617466039168903
1105	42-43	-0.10008499990718889
1105	44-45	-0.06605763001011411
1105	46-47	-0.10949694346845718
1105	48-49	-0.14516043094343445
1105	50-51	-0.07881698941488935
1105	52-53	-0.03423018306187231
1105	54-55	-0.020803308554540934
1105	56-57	0.011466310580708239
1105	58-59	0.0072935503057678375
1105	60-61	-0.06291012359069015
1105	62-63	-0.07309165941939
1105	64-65	-0.11353855567794824
1105	66-67	-0.1424610112525997
1105	68-69	-0.012794678614433508
1105	70-71	-0.04008834910365522
1105	72-73	0.0030982055309820566
1105	74-75	-0.0732643284825798
1105	76-77	-0.1310920536229574
1105	78-79	-0.051430092109860936
1105	80-81	-0.0026200526010029535
1105	82-83	-0.04496398025984405
1105	84-85	-0.036261600294309915
1105	86-87	-0.08438784524784637
1105	88-89	-0.041508592714016856
1105	90-91	0.01242899045919188
1105	92-93	-0.03600997596564426
1105	94-95	-0.012096352850317516
1105	96-97	0.022737512095442725
1105	98-99	-0.020067680297096047
1105	100	-0.012901934325931563
1106	1	-0.1579572770187383
1106	2	-0.11719151678797601
1106	3	-0.12051769117285005
1106	4	-0.05421909711567707
1106	5	-0.08702668271829594
1106	6	-0.08380351047090073
1106	7	-0.08097402644010998
1106	8	-0.06517153555957833
1106	9	-0.10697744955238164
1106	10-11	-0.11507385714844531
1106	12-13	-0.07861300025118823
1106	14-15	-0.10087704176301315
1106	16-17	-0.14139073547819692
1106	18-19	-0.1072952602142081
1106	20-21	-0.08645199234250356
1106	22-23	-0.11558662603848546
1106	24-25	-0.05819951553134928
1106	26-27	-0.0901662165521202
1106	28-29	-0.13713936488501588
1106	30-31	-0.2412861398115851
1106	32-33	-0.23240693306911453
1106	34-35	-0.20373605099504033
1106	36-37	-0.204155503460413
1106	38-39	-0.2354198751098906
1106	40-41	-0.2862684526126884
1106	42-43	-0.26414640368746944
1106	44-45	-0.2549295175810258
1106	46-47	-0.3135371518006451
1106	48-49	-0.2683836390400671
1106	50-51	-0.4250371799106887
1106	52-53	-0.40137314884716346
1106	54-55	-0.39996688594154506
1106	56-57	-0.3538509536953356
1106	58-59	-0.36866304051327603
1106	60-61	-0.35098503526784697
1106	62-63	-0.4237199260977462
1106	64-65	-0.4027203892594855
1106	66-67	-0.35333666625400895
1106	68-69	-0.4200957253661599
1106	70-71	-0.3581683295572162
1106	72-73	-0.3551044896611373
1106	74-75	-0.3525928848095248
1106	76-77	-0.3474828872349818
1106	78-79	-0.3935244797963904
1106	80-81	-0.3350291321012975
1106	82-83	-0.356120648251121
1106	84-85	-0.3082741067115151
1106	86-87	-0.29971843052955194
1106	88-89	-0.30006179594404614
1106	90-91	-0.3567179521640469
1106	92-93	-0.3243113627911356
1106	94-95	-0.3536133930210035
1106	96-97	-0.3468344653007591
1106	98-99	-0.38362071957881483
1106	100	-0.30512793853725384
1107	1	-0.2333067264500741
1107	2	-0.17643908127231356
1107	3	-0.14922648149434536
1107	4	-0.17408233867796952
1107	5	-0.2280913896550203
1107	6	-0.19956177784690254
1107	7	-0.14219015205024732
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2216	30-31	-0.05626112856946719
2216	32-33	0.04727968955358364
2216	34-35	0.10444347818704358
2216	36-37	0.07590329362292181
2216	38-39	0.08666867213960217
2216	40-41	0.09596023404826326
2216	42-43	0.13362375976785046
2216	44-45	0.13806997549410482
2216	46-47	0.17136826034930408
2216	48-49	0.16400299396310913
2216	50-51	0.1922620812458007
2216	52-53	0.1354435790026045
2216	54-55	0.12716219852224953
2216	56-57	0.05710478472776259
2216	58-59	0.1022194327270185
2216	60-61	0.008761619724431569
2216	62-63	0.022102198580356003
2216	64-65	0.0724309129711429
2216	66-67	0.07274459301000036
2216	68-69	0.09235391384384428
2216	70-71	0.05203718852234829
2216	72-73	0.09980556726675616
2216	74-75	0.07033652635951171
2216	76-77	0.13179533857270087
2216	78-79	0.1330320139102099
2216	80-81	0.11331734830385898
2216	82-83	0.17504285311740375
2216	84-85	0.14931092952914327
2216	86-87	0.17637304907597695
2216	88-89	0.1588065019036975
2216	90-91	0.20117814745268703
2216	92-93	0.1820305866044194
2216	94-95	0.17542011648210476
2216	96-97	0.17658229170785944
2216	98-99	0.1452280307537741
2216	100	0.20754370906109898
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43.0
3	252.0
4	63.0
5	115.0
6	320.0
7	745.0
8	1425.0
9	2485.0
10	3572.0
11	5101.0
12	7956.0
13	11701.0
14	13070.0
15	17707.0
16	22944.0
17	36299.0
18	42184.0
19	20909.0
20	12483.0
21	10371.0
22	10118.0
23	10289.0
24	11124.0
25	12783.0
26	15656.0
27	19931.0
28	24617.0
29	31411.0
30	39102.0
31	50042.0
32	63441.0
33	82792.0
34	114878.0
35	189429.0
36	297115.0
37	612458.0
38	1179212.0
39	791493.0
40	8264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83372349328213	21.00819203395319	15.15679978037272	22.00128469239196
2	14.094062348376182	22.01428771685104	41.443812502798956	22.44783743197382
3	15.933292685285805	29.08473793006045	32.450046490873135	22.53192289378061
4	10.49360078433451	18.924984763772226	40.35387795119107	30.22753650070219
5	10.798926056076668	39.490608292526716	35.732361255733956	13.978104395662658
6	26.419936935265902	40.81737724899971	18.821590397201835	13.941095418532553
7	23.432390895360236	35.86515275974456	23.806168684914812	16.89628765998039
8	24.65982140491269	35.465884098677755	21.269111529187313	18.605182967222238
9	25.836787321755917	15.834662198077426	22.46772435291044	35.860826127256225
10-11	22.40870187339357	27.904435729616576	29.811070775590238	19.875791621399614
12-13	24.322716553167812	26.40276636900819	29.93435173163041	19.340165346193594
14-15	21.49454145578844	27.17480590370704	29.132700919473226	22.197951721031295
16-17	18.70191314025279	30.54793449746946	29.632329950449137	21.117822411828612
18-19	18.682854535472096	27.890182983314855	34.11534128126477	19.311621199948277
20-21	20.559010572617186	26.84008585283129	33.94053896499643	18.660364609555103
22-23	19.973131243541165	26.93187418850526	34.150176210286446	18.944818357667135
24-25	19.46873519021903	27.133631936939494	34.09790059327229	19.29973227956918
26-27	19.251148083797727	27.116426335959886	34.12668655238868	19.505739027853704
28-29	19.19433624866832	27.13573471648452	34.27038037231831	19.399548662528854
30-31	19.441933771767005	27.518032459354263	33.873836776863335	19.1661969920154
32-33	19.175296441064344	27.286131706772892	34.254951106998604	19.28362074516416
34-35	19.442306938133502	27.15349344492176	33.97359155116183	19.430608065782906
36-37	19.594468370721657	27.32864299983492	33.56779701213161	19.509091617311817
38-39	19.543386194666347	27.450791528849383	33.56413546580531	19.441686810678966
40-41	19.49715082163123	27.335723436926568	33.549144849022035	19.617980892420164
42-43	19.49546170711039	27.444429958933576	33.279834102037	19.780274231919034
44-45	19.825146122281122	27.585684706565683	32.85249930178219	19.736669869371
46-47	19.970961032429496	27.944130393682176	32.14979910010733	19.935109473780997
48-49	20.117888890581806	27.99662417517192	31.539093919606877	20.346393014639393
50-51	20.15252710965208	28.182829557804666	31.265235454219408	20.399407878323846
52-53	20.1385075205866	28.336368896209983	31.194174581057215	20.330949002146205
54-55	20.2508406157551	28.549562047770323	30.992398917768902	20.207198418705676
56-57	20.15364533717948	28.22627252325958	31.011844493949237	20.608237645611702
58-59	20.067776337027894	27.951035054473728	31.404374300372545	20.576814308125833
60-61	20.20928188200005	28.25938711252346	31.084652772395355	20.44667823308114
62-63	20.080114249056106	28.093314525006953	31.349250985827375	20.477320240109563
64-65	20.094346809650908	27.95614814343736	31.431094434916133	20.5184106119956
66-67	19.984111876134108	28.068599280105822	31.222097741109433	20.725191102650637
68-69	20.431841000093005	28.183393841207305	30.937449405639644	20.44731575306004
70-71	20.360264872585052	28.01642337217036	30.942224071183844	20.681087684060746
72-73	20.261252596951877	28.162213288629744	31.03203308093719	20.544501033481186
74-75	20.36398363116354	28.252949849869307	30.769578299884326	20.613488219082836
76-77	20.38090551741837	28.23353605597217	30.663809656677866	20.7217487699316
78-79	20.43704743897477	28.22054392712838	30.851358267687846	20.491050366209006
80-81	20.307392513577565	28.189376852209087	30.846992160836407	20.656238473376945
82-83	20.19593526343201	28.22464918769262	31.04817270751595	20.531242841359422
84-85	20.200466048519075	28.17088145387635	30.809323408206357	20.819329089398217
86-87	20.301874573210853	28.268883589806503	30.650873823042385	20.778368013940256
88-89	20.126875753789335	28.08075679753525	30.81101935835014	20.981348090325277
90-91	20.06426051649546	28.062655792204104	30.77323809566711	21.099845595633326
92-93	20.06486727117593	28.166161436831256	30.750571033371664	21.01840025862115
94-95	19.852032842609116	28.091130661881493	30.826181980445575	21.230654515063815
96-97	19.819215979880735	28.18024505448018	30.733747132107926	21.266791833531162
98-99	19.860854486788305	28.10770104382983	30.70075420832239	21.330690261059473
100	19.943273446472	28.132242188042376	30.519882116455843	21.404602249029782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4217.0
1	2392.5
2	2553.5
3	4365.5
4	2630.0
5	1455.5
6	3421.5
7	9703.5
8	17454.0
9	18900.0
10	11924.5
11	5517.0
12	4542.0
13	4847.0
14	5427.0
15	6006.5
16	6577.5
17	7242.0
18	8135.0
19	9200.5
20	10697.5
21	13078.5
22	16948.5
23	22520.5
24	28845.0
25	35364.5
26	44066.5
27	54969.0
28	68478.5
29	81653.0
30	90455.0
31	99276.5
32	108683.5
33	117370.0
34	124490.5
35	129355.0
36	131407.0
37	132114.5
38	132608.0
39	130815.0
40	129303.5
41	126627.5
42	121430.0
43	117044.5
44	113451.0
45	110641.5
46	108792.5
47	105682.0
48	101575.5
49	99089.5
50	97525.0
51	93047.5
52	86127.0
53	80632.0
54	77326.0
55	74072.0
56	67269.0
57	59486.5
58	54013.0
59	48974.0
60	42193.5
61	34405.0
62	28301.0
63	22619.0
64	17069.0
65	13072.0
66	9915.0
67	7324.5
68	5494.5
69	4025.5
70	2810.5
71	2010.0
72	1453.0
73	1044.0
74	814.5
75	598.0
76	354.0
77	251.0
78	212.5
79	122.0
80	45.5
81	21.0
82	12.0
83	8.5
84	5.0
85	2.5
86	0.5
87	1.0
88	1.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006200482259731313
2	0.0044781260764726145
3	0.0010864092848247172
4	0.0
5	0.043350380243249685
6	0.0
7	0.0
8	0.0
9	5.2995574869498396E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.0599114973899679E-4
20-21	0.0
22-23	0.0
24-25	1.3248893717374599E-5
26-27	7.949336230424759E-5
28-29	1.457378308911206E-4
30-31	2.914756617822412E-4
32-33	0.0012453960094332124
34-35	8.479291979119743E-4
36-37	0.0010334137099552187
38-39	8.61178091629349E-4
40-41	1.32488937173746E-4
42-43	3.709690240864888E-4
44-45	1.5898672460849518E-4
46-47	1.457378308911206E-4
48-49	1.98733405760619E-4
50-51	3.5772013036911417E-4
52-53	6.756935795861046E-4
54-55	7.816847293251013E-4
56-57	0.0020933252073451866
58-59	4.372134926733618E-4
60-61	0.001126155965976841
62-63	0.0010466626036725933
64-65	0.001523622777498079
66-67	3.1797344921699036E-4
68-69	2.914756617822412E-4
70-71	2.64977874347492E-4
72-73	1.1924004345637139E-4
74-75	5.697024298471078E-4
76-77	6.491957921513553E-4
78-79	8.081825167598506E-4
80-81	7.419380481729776E-4
82-83	0.0013646360528895836
84-85	0.0011659026471289647
86-87	0.002146320782214685
88-89	0.0013381382654548345
90-91	0.0011394048596942157
92-93	0.001059911497389968
94-95	5.829513235644823E-4
96-97	1.1924004345637139E-4
98-99	2.3848008691274277E-4
100	1.5898672460849518E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3773900.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.49649544110925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63423157469843	42.48847996177821
2	16.795729827492732	19.649826665680628
3	5.352034095606167	9.392257142228624
4	2.130005577342593	4.983914461782331
5	1.0235107674052586	2.993589646972398
6	0.5909504901740127	2.0741119592631247
7	0.3427896817181334	1.4036396537718858
8	0.22876406163649057	1.0705516710886887
9	0.15754947873167133	0.8294483127938717
>10	0.6770208793772831	6.823479349943525
>50	0.03867218399476678	1.552280984249357
>100	0.026058748061504558	2.8729666020168105
>500	0.0016823296739095868	0.6667702827201929
>1k	7.274938814774002E-4	0.7449932181381155
>5k	1.364051027770122E-4	0.431383256157875
>10k+	1.364051027770122E-4	2.0223068314142587
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32345	0.8570709345769627	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25837	0.684623333951615	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17852	0.4730385012851427	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5639	0.14942102334455074	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5518	0.1462147910649461	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5062	0.13413179999470043	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4758	0.1260764726145367	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4064	0.10768700813482074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.6497787434749198E-5	0.0
5	0.0	0.0	0.0	2.6497787434749198E-5	0.0
6	0.0	0.0	0.0	2.6497787434749198E-5	0.0
7	0.0	0.0	0.0	2.6497787434749198E-5	0.0
8	0.0	0.0	0.0	2.6497787434749198E-5	0.0
9	0.0	0.0	0.0	7.949336230424759E-5	0.0
10-11	0.0	0.0	0.0	7.949336230424759E-5	0.0
12-13	0.0	0.0	0.0	7.949336230424759E-5	0.0
14-15	0.0	0.0	0.0	7.949336230424759E-5	0.0
16-17	0.0	0.0	0.0	9.274225602162218E-5	0.0
18-19	0.0	0.0	0.0	1.32488937173746E-4	0.0
20-21	0.0	0.0	0.0	2.64977874347492E-4	0.0
22-23	0.0	0.0	0.0	3.8421791780386336E-4	0.0
24-25	0.0	0.0	0.0	6.756935795861046E-4	0.0
26-27	0.0	0.0	0.0	8.876758790640982E-4	0.0
28-29	0.0	0.0	0.0	0.0016428628209544502	0.0
30-31	0.0	0.0	0.0	0.004239645989559872	0.0
32-33	0.0	0.0	0.0	0.008770767640901985	0.0
34-35	0.0	0.0	0.0	0.01506399215665492	0.0
36-37	0.0	0.0	0.0	0.026047325048358463	0.0
38-39	2.6497787434749198E-5	0.0	0.0	0.04246270436418559	0.0
40-41	2.6497787434749198E-5	0.0	0.0	0.06346220090622433	0.0
42-43	2.6497787434749198E-5	0.0	0.0	0.0825671056466785	0.0
44-45	2.6497787434749198E-5	0.0	0.0	0.10685232783062615	0.0
46-47	2.6497787434749198E-5	0.0	0.0	0.13639736082037152	0.0
48-49	2.6497787434749198E-5	0.0	0.0	0.17259333845623892	0.0
50-51	2.6497787434749198E-5	0.0	0.0	0.2095312541402793	0.0
52-53	2.6497787434749198E-5	0.0	0.0	0.24619094305625477	0.0
54-55	2.6497787434749198E-5	0.0	0.0	0.2862290998701609	0.0
56-57	3.9746681152123795E-5	0.0	0.0	0.3364821537401627	0.0
58-59	5.2995574869498396E-5	0.0	0.0	0.3964201489175654	0.0
60-61	5.2995574869498396E-5	0.0	0.0	0.4614324703887226	0.0
62-63	5.2995574869498396E-5	0.0	0.0	0.5306446911682874	0.0
64-65	5.2995574869498396E-5	0.0	0.0	0.5987307559818755	0.0
66-67	5.2995574869498396E-5	0.0	0.0	0.6808076525610112	0.0
68-69	5.2995574869498396E-5	0.0	0.0	0.7816582315376666	0.0
70-71	5.2995574869498396E-5	0.0	0.0	0.8938896102175469	0.0
72-73	5.2995574869498396E-5	0.0	0.0	1.0135668671665916	0.0
74-75	5.2995574869498396E-5	0.0	0.0	1.1326479238983547	0.0
76-77	5.2995574869498396E-5	0.0	0.0	1.2633482604202548	0.0
78-79	5.2995574869498396E-5	0.0	0.0	1.4204403932271656	0.0
80-81	5.2995574869498396E-5	0.0	0.0	1.5964916929436392	0.0
82-83	5.2995574869498396E-5	0.0	0.0	1.7899255412173085	0.0
84-85	5.2995574869498396E-5	0.0	0.0	1.9790137523516786	0.0
86-87	5.2995574869498396E-5	0.0	0.0	2.1810063859667714	0.0
88	5.2995574869498396E-5	0.0	0.0	2.350115265375341	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11640	0.0	64.88812	1
GTATCAA	22970	0.0	45.842228	1
GTGGTAT	3725	0.0	36.234997	1
TGGTATC	3770	0.0	35.175945	2
ATCAACG	30575	0.0	34.264664	3
TATCAAC	30645	0.0	34.220264	2
TCAACGC	30885	0.0	33.935966	4
CAACGCA	32025	0.0	32.77199	5
AACGCAG	32920	0.0	32.00773	6
TACCTGG	2680	0.0	29.829927	2
ACGCAGA	36735	0.0	28.542933	7
CGCAGAG	36765	0.0	28.494078	8
GCAGAGT	39320	0.0	26.475203	9
GTACATG	20740	0.0	26.462734	1
TACATGG	20895	0.0	25.769215	2
CATGGGT	9730	0.0	25.758	4
GTACCTG	3265	0.0	25.207365	1
ACATGGG	20725	0.0	25.002579	3
GAGTACT	27430	0.0	23.267675	12-13
AGAGTAC	37815	0.0	22.78746	10-11
>>END_MODULE