##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431415_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4033895
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.169446155638656	31.0	31.0	34.0	26.0	34.0
2	30.281554923963068	31.0	31.0	34.0	26.0	34.0
3	30.352673532652684	31.0	31.0	34.0	26.0	34.0
4	33.67799037902573	37.0	35.0	37.0	28.0	37.0
5	33.75181803195175	37.0	35.0	37.0	28.0	37.0
6	33.94057009416457	37.0	35.0	37.0	30.0	37.0
7	33.89526574191941	37.0	35.0	37.0	29.0	37.0
8	33.858889237325215	37.0	35.0	37.0	29.0	37.0
9	35.304366375426234	39.0	37.0	39.0	28.0	39.0
10-11	35.30576539548005	39.0	37.0	39.0	28.0	39.0
12-13	35.23128452773312	39.0	37.0	39.0	27.5	39.0
14-15	36.50492452084152	40.0	38.0	41.0	27.0	41.0
16-17	36.51078188698516	40.0	38.0	41.0	27.0	41.0
18-19	36.51986070534806	40.0	37.5	41.0	27.0	41.0
20-21	36.60534731320473	40.0	38.0	41.0	28.0	41.0
22-23	36.5722787777074	40.0	37.0	41.0	28.0	41.0
24-25	36.619656932071855	40.0	37.0	41.0	28.0	41.0
26-27	36.659003518931456	40.0	37.0	41.0	29.0	41.0
28-29	36.627999861176356	40.0	37.0	41.0	29.0	41.0
30-31	36.561297083835846	40.0	37.0	41.0	29.5	41.0
32-33	36.51341457821783	40.0	37.0	41.0	29.0	41.0
34-35	36.44452532849765	40.0	37.0	41.0	29.0	41.0
36-37	36.304194085369105	40.0	37.0	41.0	28.5	41.0
38-39	36.173288347862304	40.0	36.0	41.0	28.0	41.0
40-41	36.10586951321241	40.0	36.0	41.0	27.5	41.0
42-43	35.933763149511826	40.0	36.0	41.0	27.0	41.0
44-45	35.76475453624846	40.0	35.5	41.0	27.0	41.0
46-47	35.622014950810566	40.0	35.0	41.0	27.0	41.0
48-49	35.47954396433224	40.0	35.0	41.0	26.0	41.0
50-51	35.3412911094612	40.0	35.0	41.0	26.0	41.0
52-53	35.173954577399755	39.0	35.0	41.0	26.0	41.0
54-55	35.00761559237412	39.0	34.5	41.0	26.0	41.0
56-57	34.81213467380782	39.0	34.0	41.0	25.0	41.0
58-59	34.59675090700179	39.0	34.0	40.5	24.5	41.0
60-61	34.36098349114194	38.0	34.0	40.0	23.5	41.0
62-63	34.26795020693399	38.0	34.0	40.0	23.5	41.0
64-65	34.02381891447348	38.0	33.5	40.0	23.0	41.0
66-67	33.77578903268429	37.0	33.0	40.0	22.5	41.0
68-69	33.48506443524187	37.0	33.0	40.0	22.0	41.0
70-71	33.15952757818436	36.5	33.0	39.5	20.5	41.0
72-73	32.82314561980418	36.0	32.0	39.0	20.0	41.0
74-75	32.53966786443375	35.5	32.0	39.0	19.0	41.0
76-77	32.27892880206352	35.0	32.0	38.5	18.5	40.0
78-79	31.923898366219248	35.0	32.0	37.0	17.0	39.5
80-81	31.585019689406888	35.0	32.0	37.0	14.0	39.0
82-83	31.242309727942846	35.0	31.5	36.5	9.5	39.0
84-85	30.927928961958603	35.0	31.0	36.0	7.0	37.5
86-87	30.633177859116312	35.0	31.0	36.0	3.5	37.0
88-89	30.351480640919014	35.0	31.0	35.5	2.0	37.0
90-91	30.10387144434845	35.0	31.0	35.0	2.0	36.5
92-93	29.85662740353926	35.0	31.0	35.0	2.0	36.0
94-95	29.626946040985203	34.0	30.5	35.0	2.0	36.0
96-97	29.405930000656934	34.0	30.0	35.0	2.0	36.0
98-99	29.171285321011084	34.0	30.0	35.0	2.0	35.0
100	28.987171703775136	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4257573945942674
1101	2	0.4362426676917863
1101	3	0.40287622940389056
1101	4	0.4734624056208787
1101	5	0.44992506483502126
1101	6	0.4489622358847001
1101	7	0.4961054262624742
1101	8	0.45397745614558715
1101	9	0.48792341701689423
1101	10-11	0.4889443262532751
1101	12-13	0.35005715170763807
1101	14-15	0.3816571720728561
1101	16-17	0.4397022383338438
1101	18-19	0.3493757698577724
1101	20-21	0.3259459806450167
1101	22-23	0.4340315615907997
1101	24-25	0.3169248185902305
1101	26-27	0.37090205515868035
1101	28-29	0.333657268861721
1101	30-31	0.3485458387207885
1101	32-33	0.34501829064831213
1101	34-35	0.3622133017971336
1101	36-37	0.39815151938545057
1101	38-39	0.3993942743256369
1101	40-41	0.29570325353220284
1101	42-43	0.33157177412859795
1101	44-45	0.25234930706457703
1101	46-47	0.29543317723922513
1101	48-49	0.30906632862006944
1101	50-51	0.3796658870464782
1101	52-53	0.3670373417315176
1101	54-55	0.37700959759658303
1101	56-57	0.2515719330205499
1101	58-59	0.28280815442950313
1101	60-61	0.2698040852507404
1101	62-63	0.29123198167575026
1101	64-65	0.3129810514223266
1101	66-67	0.33758311805841146
1101	68-69	0.31293271185187166
1101	70-71	0.22522630140194622
1101	72-73	0.26501943866794875
1101	74-75	0.344926179258394
1101	76-77	0.3024286341105764
1101	78-79	0.28842294376823574
1101	80-81	0.31188337017056256
1101	82-83	0.3132744297862011
1101	84-85	0.21825619981569488
1101	86-87	0.2470901069128999
1101	88-89	0.23645457087880573
1101	90-91	0.18070179288503496
1101	92-93	0.10600311678831176
1101	94-95	0.14030517911233176
1101	96-97	0.17004883765328316
1101	98-99	0.1635338607175214
1101	100	0.15918320541205944
1102	1	0.10427235658445966
1102	2	0.12616257965117228
1102	3	0.09080037848322675
1102	4	0.16136452328305495
1102	5	0.13791282741643585
1102	6	0.24185356583097928
1102	7	0.12370932713456995
1102	8	0.13012466097893594
1102	9	0.11638358988496122
1102	10-11	0.11860996612595898
1102	12-13	0.09450154299316438
1102	14-15	0.16524920622120476
1102	16-17	0.1073805782258006
1102	18-19	0.09872811707196405
1102	20-21	0.09475301425467819
1102	22-23	0.14190622208252535
1102	24-25	0.16270212242675086
1102	26-27	0.15414149803434896
1102	28-29	0.06329232463841805
1102	30-31	0.1455850702366135
1102	32-33	0.16957677041673236
1102	34-35	0.12519308463469514
1102	36-37	0.04062777861383893
1102	38-39	0.04801726940760176
1102	40-41	0.05170604685601177
1102	42-43	0.014759316183209137
1102	44-45	0.0769194227289347
1102	46-47	0.07020006358215625
1102	48-49	-0.04108861189864399
1102	50-51	-0.06391939119436785
1102	52-53	-0.030233999328522998
1102	54-55	-0.05819848529919369
1102	56-57	-0.03893557396335012
1102	58-59	-0.047999126310052986
1102	60-61	-0.056361890586288155
1102	62-63	0.02642571757092327
1102	64-65	0.006331957911775987
1102	66-67	0.04819708699282188
1102	68-69	0.05129114564374504
1102	70-71	0.0555010479402398
1102	72-73	0.02484486006457587
1102	74-75	-0.02364631721658128
1102	76-77	-0.09040830835871816
1102	78-79	-0.08422693011518945
1102	80-81	-0.07876555134136254
1102	82-83	-0.07958274260367304
1102	84-85	-0.12585188454490392
1102	86-87	-0.20016969275595642
1102	88-89	-0.11801309337987931
1102	90-91	-0.1280190512218411
1102	92-93	-0.1876119127255187
1102	94-95	-0.13447576481044266
1102	96-97	-0.13852251177042874
1102	98-99	-0.15551508139643389
1102	100	-0.1729125883710374
1103	1	-0.24550314258763706
1103	2	-0.24477877934856807
1103	3	-0.08560151059320731
1103	4	0.027908634661940823
1103	5	3.670823013450786E-4
1103	6	0.14974955630736986
1103	7	0.16344853108682145
1103	8	0.1094081036793284
1103	9	0.2446534441926005
1103	10-11	0.11807942809620897
1103	12-13	0.20548678922197894
1103	14-15	0.21707770704509244
1103	16-17	0.14025663591704784
1103	18-19	0.1655969698762405
1103	20-21	0.10487911983475584
1103	22-23	0.09016973801521289
1103	24-25	0.17337406037486147
1103	26-27	0.14218007185986892
1103	28-29	0.10690049472349017
1103	30-31	0.12597848073364304
1103	32-33	0.1129932347468241
1103	34-35	0.11250053894474377
1103	36-37	0.039499507756623586
1103	38-39	0.15039481190330406
1103	40-41	0.15760855823650388
1103	42-43	0.1030883821454367
1103	44-45	0.0029005921390492517
1103	46-47	-0.06870612545987598
1103	48-49	0.04695186745099278
1103	50-51	-0.04237092218270533
1103	52-53	-0.010288176387817316
1103	54-55	-0.02268001726034896
1103	56-57	-0.07150173325914722
1103	58-59	-0.06997533534002542
1103	60-61	-0.04477655469754893
1103	62-63	-0.04589569219599099
1103	64-65	-0.054057109996094255
1103	66-67	0.04771744651507248
1103	68-69	0.07267012329997158
1103	70-71	0.03707638484191733
1103	72-73	0.007612904625759143
1103	74-75	-0.018396347554521242
1103	76-77	-0.025196509353399676
1103	78-79	-0.016557255729146902
1103	80-81	-0.06329018826379951
1103	82-83	-0.0470068049604464
1103	84-85	-0.15971480314592768
1103	86-87	-0.10294283208687816
1103	88-89	-0.16766779235220142
1103	90-91	-0.10120832693212378
1103	92-93	-0.18633019703172238
1103	94-95	-0.21206945892969742
1103	96-97	-0.18277680496640158
1103	98-99	-0.20803784488779797
1103	100	-0.2338602757166548
1104	1	-0.44225825794579876
1104	2	-0.4176465022261233
1104	3	-0.3913206882511915
1104	4	-0.35712856580173025
1104	5	-0.2860041149204591
1104	6	-0.22171247131722538
1104	7	-0.20663773914282046
1104	8	-0.30053032307925776
1104	9	-0.2257454696237602
1104	10-11	-0.20472854991260903
1104	12-13	-0.22499841168878731
1104	14-15	-0.29359719218361136
1104	16-17	-0.2765449342968509
1104	18-19	-0.25525896150953997
1104	20-21	-0.3021730921269068
1104	22-23	-0.21519126701752356
1104	24-25	-0.23982058821557217
1104	26-27	-0.25428961905384995
1104	28-29	-0.23620146048963164
1104	30-31	-0.22207082473189388
1104	32-33	-0.266591443187707
1104	34-35	-0.23399507136157638
1104	36-37	-0.31051948545321295
1104	38-39	-0.260218525133304
1104	40-41	-0.2437782281304237
1104	42-43	-0.2820917617039882
1104	44-45	-0.3379990870176073
1104	46-47	-0.4182088389126477
1104	48-49	-0.46500520428919856
1104	50-51	-0.4428611429767315
1104	52-53	-0.4528095098739797
1104	54-55	-0.36101711056301156
1104	56-57	-0.3483977554378228
1104	58-59	-0.47317351331781055
1104	60-61	-0.5167617882619808
1104	62-63	-0.38256951301047337
1104	64-65	-0.3701746048068344
1104	66-67	-0.3649382711999962
1104	68-69	-0.4058427903671671
1104	70-71	-0.45559916234251574
1104	72-73	-0.5057715074154743
1104	74-75	-0.3977466489307133
1104	76-77	-0.38476033197589743
1104	78-79	-0.33978337803390346
1104	80-81	-0.3696827641867735
1104	82-83	-0.40988090388064435
1104	84-85	-0.4328172864005282
1104	86-87	-0.35182024964305825
1104	88-89	-0.46312579534703247
1104	90-91	-0.5180465780006109
1104	92-93	-0.48923669606499587
1104	94-95	-0.5021896633840974
1104	96-97	-0.5867245924813496
1104	98-99	-0.5273047174557455
1104	100	-0.5250900069638966
1105	1	-0.2061293623240701
1105	2	-0.19476564625298565
1105	3	-0.11249825788665291
1105	4	0.005459894825285971
1105	5	-0.07747221049366004
1105	6	-0.08055320509171082
1105	7	0.0502663492412978
1105	8	-0.048507686617156764
1105	9	-0.06840168473834751
1105	10-11	-0.12306496151554569
1105	12-13	-0.10520754553927958
1105	14-15	-0.08381127628230445
1105	16-17	-0.16439328295471256
1105	18-19	-0.13339336905873012
1105	20-21	-0.19672342401971576
1105	22-23	-0.18908466207731323
1105	24-25	-0.26706481639580204
1105	26-27	-0.21733637602442002
1105	28-29	-0.22023523937957634
1105	30-31	-0.16156749123921088
1105	32-33	-0.12623956946993076
1105	34-35	-0.18966447634311834
1105	36-37	-0.18092609119875647
1105	38-39	-0.17475314954071308
1105	40-41	-0.3052859188353523
1105	42-43	-0.27333672770937767
1105	44-45	-0.3568931340571311
1105	46-47	-0.2889764323843522
1105	48-49	-0.43524909782647825
1105	50-51	-0.3426763182890298
1105	52-53	-0.2947426786102767
1105	54-55	-0.32844978059050334
1105	56-57	-0.3470519013520814
1105	58-59	-0.2812236981082279
1105	60-61	-0.36473061009434105
1105	62-63	-0.324433354283137
1105	64-65	-0.3069459360671942
1105	66-67	-0.32376245274268456
1105	68-69	-0.27319160260745434
1105	70-71	-0.1867673575204023
1105	72-73	-0.20442009308209208
1105	74-75	-0.25098091390109545
1105	76-77	-0.24071941814877107
1105	78-79	-0.2683606263230338
1105	80-81	-0.25423908641108994
1105	82-83	-0.22409139755010088
1105	84-85	-0.238593766844307
1105	86-87	-0.29990942641969554
1105	88-89	-0.2619558215752953
1105	90-91	-0.30032334433878205
1105	92-93	-0.31609071309117454
1105	94-95	-0.3724767966283622
1105	96-97	-0.33951285533833087
1105	98-99	-0.494198797394116
1105	100	-0.4400258430769277
1106	1	-0.185463699371212
1106	2	-0.13986521550823738
1106	3	-0.19985020119985109
1106	4	-0.03329775957720216
1106	5	0.02712259484793833
1106	6	0.012405356502519282
1106	7	0.06211819899173321
1106	8	0.032018986114955794
1106	9	-0.0015951967999328076
1106	10-11	0.006245531487934386
1106	12-13	-0.06882211342946931
1106	14-15	-0.03062070238095771
1106	16-17	0.005973813522189175
1106	18-19	-0.09912843812050909
1106	20-21	-0.14000232963122983
1106	22-23	-0.053820486872623974
1106	24-25	-0.016364889554985496
1106	26-27	-0.11075177647863654
1106	28-29	-0.09239599590521408
1106	30-31	-0.06459440816904305
1106	32-33	-0.08939479255316485
1106	34-35	-0.04924234749083212
1106	36-37	-0.10351323140206858
1106	38-39	-0.13078785365207324
1106	40-41	-0.1275110095000116
1106	42-43	-0.16342739570750808
1106	44-45	-0.16519942763862616
1106	46-47	-0.1565444500707187
1106	48-49	-0.04002892883053022
1106	50-51	-0.13383947318113343
1106	52-53	-0.17855952342446102
1106	54-55	-0.19826199755199525
1106	56-57	-0.16427235726644085
1106	58-59	-0.24906537577213328
1106	60-61	-0.2647348154126661
1106	62-63	-0.26848730951852673
1106	64-65	-0.18394734217879716
1106	66-67	-0.08454750891038287
1106	68-69	-0.14451934731748395
1106	70-71	-0.19299416290916582
1106	72-73	-0.26115295234922087
1106	74-75	-0.23614590255674983
1106	76-77	-0.22451449376551125
1106	78-79	-0.21651369612753868
1106	80-81	-0.21914343822579596
1106	82-83	-0.2680397831639958
1106	84-85	-0.22105190167660282
1106	86-87	-0.3119837658787752
1106	88-89	-0.3245592996628055
1106	90-91	-0.28325968115941436
1106	92-93	-0.2770212337785587
1106	94-95	-0.22895296738119697
1106	96-97	-0.24463333659585018
1106	98-99	-0.2825934927744278
1106	100	-0.36256799583055255
1107	1	-0.5163083276199742
1107	2	-0.4963333147818041
1107	3	-0.4164590279459084
1107	4	-0.26988324632770855
1107	5	-0.26775533415536756
1107	6	-0.38435850075909883
1107	7	-0.301208961422482
1107	8	-0.3643784417418985
1107	9	-0.32711829185341657
1107	10-11	-0.21840054579921286
1107	12-13	-0.2877590375122949
1107	14-15	-0.28313010166408503
1107	16-17	-0.3956383064432458
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2216	82-83	0.46667382451734696
2216	84-85	0.45122353257806935
2216	86-87	0.42661783137094744
2216	88-89	0.43563193246662735
2216	90-91	0.4995709192537383
2216	92-93	0.47977888842563843
2216	94-95	0.417495296812497
2216	96-97	0.41331349974652554
2216	98-99	0.3397110263365626
2216	100	0.4142486704648398
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111697.0
3	19026.0
4	13863.0
5	12207.0
6	9029.0
7	8089.0
8	8613.0
9	9503.0
10	10090.0
11	11189.0
12	12239.0
13	13381.0
14	14477.0
15	14525.0
16	14622.0
17	14267.0
18	13943.0
19	13603.0
20	13596.0
21	14294.0
22	16407.0
23	23897.0
24	36877.0
25	48577.0
26	50847.0
27	41872.0
28	41407.0
29	50295.0
30	59190.0
31	68376.0
32	86783.0
33	114875.0
34	160466.0
35	244217.0
36	400197.0
37	707645.0
38	1037834.0
39	490340.0
40	1540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.784937269873026	21.81207012026513	14.301341519047256	22.10165109081459
2	14.813005739725286	22.413783684320574	40.742141463344375	22.031069112609764
3	17.124612445648015	28.832528836229294	30.818182992212655	23.22467572591004
4	11.550728038709533	19.613326827112072	39.808910791511614	29.02703434266678
5	11.928715212280991	40.14765105671713	35.011828228050724	12.91180550295116
6	27.34874503705894	40.62357661213096	19.25943641034852	12.768241940461579
7	24.502332043266843	35.2450133968443	23.598491614567827	16.654162945321026
8	23.57556287006119	37.333770913751756	21.599108367882096	17.491557848304957
9	24.61056145478033	18.525693481026583	21.93193665200797	34.93180841218512
10-11	21.83959928474182	29.341809658763896	29.770748738771996	19.04784231772229
12-13	22.876445290328213	27.606422090267884	29.10073222089474	20.416400398509165
14-15	20.795368721505223	29.157404849754222	26.647513967301574	23.399712461438984
16-17	19.940782870691244	32.10093305676182	27.179848712538444	20.778435360008494
18-19	19.987298712816333	30.870366867501637	29.16660826637973	19.975726153302304
20-21	21.822518408152366	29.96051998169697	28.284247201054125	19.93271440909654
22-23	21.33707605112925	30.060849139332845	28.330485073988175	20.271589735549725
24-25	20.94431740547449	30.03367042623738	28.407341457763497	20.61467071052463
26-27	20.70423108238249	29.888491080824554	28.50882589783422	20.898451938958733
28-29	20.709180201717803	29.903385621262007	28.61860490385076	20.768829273169427
30-31	20.964176707305544	30.370026909830113	28.207515550617003	20.45828083224734
32-33	20.531157292481836	30.065488866818217	28.706753340152996	20.69660050054695
34-35	20.779426328250743	29.77343677127553	28.60239205202857	20.844744848445156
36-37	21.11163350369672	29.991787823152883	28.213665459236836	20.68291321391356
38-39	20.887725285515884	30.068393812205795	28.433596128187787	20.610284774090534
40-41	20.725885786788492	29.812997017969707	28.66689812411552	20.794219071126278
42-43	20.659332453366666	29.815017212936922	28.601794491934612	20.9238558417618
44-45	20.905670348646797	29.909426762338605	28.48878782465438	20.696115064360217
46-47	20.903966295641528	30.109749544384833	28.329972556411683	20.65631160356196
48-49	20.770661266979	29.78573804146203	28.479457232645185	20.964143458913785
50-51	20.754681873270307	29.69192734273533	28.511277803758894	21.042112980235466
52-53	20.79229708548159	29.77864614990724	28.456000889156606	20.973055875454563
54-55	20.957060030513247	30.05849964094402	28.269322874626134	20.7151174539166
56-57	20.802223405019358	29.793860455931497	28.25111645947112	21.152799679578024
58-59	20.69581445245654	29.52957759722486	28.548270269995353	21.226337680323255
60-61	20.98629977256221	29.889010109104397	28.11792280511986	21.006767313213533
62-63	20.854399288331354	29.79745302107532	28.29165663525953	21.0564910553338
64-65	20.84423167685954	29.549944461706485	28.501966942466545	21.103856918967427
66-67	20.66340542012923	29.667370714869406	28.279616787406713	21.389607077594647
68-69	21.170605345959732	29.793522764864672	28.08863402129309	20.947237867882503
70-71	21.08755727217025	29.542194736541816	28.248221989040456	21.122026002247477
72-73	20.86030462186698	29.630654482321177	28.504663392117617	21.004377503694226
74-75	20.889371306746686	29.661156857703396	28.365041309613158	21.084430525936764
76-77	20.941383051274666	29.73954445397912	28.18067383975634	21.138398654989867
78-79	21.05674964233627	29.71702454539433	28.28081272709817	20.945413085171225
80-81	20.87823659501496	29.55041082173775	28.427082925009096	21.144269658238194
82-83	20.790352962629637	29.606572608631197	28.561758071088722	21.041316357650437
84-85	20.838685091352247	29.563946571819084	28.335620416478214	21.26174792035046
86-87	21.009001601944266	29.649142698079032	28.25774357826764	21.08411212170906
88-89	20.88560675960621	29.529450468150415	28.327758135228347	21.25718463701503
90-91	20.779651076390707	29.42667585274065	28.356053319254855	21.437619751613788
92-93	20.9399925181044	29.590512579309085	28.20601459068871	21.2634803118978
94-95	20.770090912304575	29.471574942958874	28.37125015426998	21.38708399046658
96-97	20.778918263345084	29.490460364825832	28.32639441704793	21.404226954781155
98-99	20.72551560362919	29.485809255373862	28.328261090151464	21.460414050845486
100	20.827855261293802	29.599080800394397	28.21752736596267	21.35553657234913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14732.0
1	10632.0
2	5681.0
3	4125.0
4	2875.5
5	2114.5
6	1876.0
7	1946.0
8	2193.5
9	2236.5
10	1809.0
11	1458.0
12	1409.5
13	1406.0
14	1509.0
15	1686.0
16	1978.5
17	2481.0
18	3182.5
19	4224.0
20	5889.5
21	8280.0
22	11994.5
23	17864.5
24	24761.5
25	32399.0
26	42628.5
27	55063.0
28	70109.0
29	84934.5
30	95399.5
31	105149.5
32	115906.0
33	125445.0
34	133269.0
35	139452.5
36	142558.0
37	143800.5
38	143772.5
39	142554.0
40	141186.0
41	137583.5
42	131891.0
43	126720.0
44	123094.5
45	119422.0
46	116559.0
47	113621.0
48	109773.5
49	107211.0
50	105512.0
51	101417.0
52	95481.0
53	90726.0
54	87065.5
55	83896.5
56	77502.5
57	69338.0
58	63690.0
59	59049.5
60	52235.0
61	43888.0
62	36908.5
63	30234.0
64	23852.5
65	19017.5
66	14953.5
67	11518.5
68	8812.0
69	6724.0
70	5064.0
71	3843.5
72	2954.5
73	2204.0
74	1739.5
75	1327.5
76	955.0
77	773.5
78	605.0
79	399.5
80	310.5
81	251.5
82	183.0
83	147.0
84	126.0
85	101.5
86	81.0
87	71.5
88	61.5
89	54.5
90	48.5
91	40.0
92	35.0
93	31.0
94	28.0
95	22.5
96	16.0
97	19.0
98	20.5
99	22.5
100	34.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17457073126593528
2	0.17038123203504305
3	0.18252830081100277
4	0.17303375521673223
5	0.18617242144379068
6	0.0748408176216783
7	0.07672485277876592
8	0.06534627202740775
9	0.05914878795804055
10-11	0.07196518501349193
12-13	0.07117190705261292
14-15	0.06900278762833441
16-17	0.06517274247346547
18-19	0.06959774609899365
20-21	0.07110993221191925
22-23	0.06886644297880833
24-25	0.06844501406209136
26-27	0.07231224412137649
28-29	0.06985804042990708
30-31	0.07501434717562058
32-33	0.07200236991790812
34-35	0.07137022654283268
36-37	0.08168284003425968
38-39	0.07736939112198012
40-41	0.07229984915323776
42-43	0.07586959997719325
44-45	0.0856492298386547
46-47	0.07637779367088136
48-49	0.08159607525728856
50-51	0.07907989672512547
52-53	0.0770719118866505
54-55	0.07899313194815433
56-57	0.07339060634944639
58-59	0.0806044778061898
60-61	0.07740657602639632
62-63	0.06954816622643872
64-65	0.0717048906825785
66-67	0.07241140386648637
68-69	0.07113472214819672
70-71	0.0670567776305531
72-73	0.06450341419397382
74-75	0.06969690584410353
76-77	0.07427064908729653
78-79	0.07124627686144533
80-81	0.07915426653395788
82-83	0.08000951933553055
84-85	0.08150931048031741
86-87	0.07853451812702116
88-89	0.08046813315666372
90-91	0.07823703889169153
92-93	0.07018030960151417
94-95	0.069721695780381
96-97	0.0685689637434787
98-99	0.0776916602935872
100	0.08507906130427291
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4033895.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.16815925520044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07479337787562	53.90383108055094
2	13.64171336650549	18.598609785634853
3	3.7991922738822037	7.769518319013874
4	1.4313973565185116	3.9030289182650733
5	0.6674179876038332	2.2748327834381743
6	0.36353275916687766	1.4868815412822085
7	0.22283280791646304	1.063307163613306
8	0.14844896595281	0.8095594201872786
9	0.10169328448776946	0.6239019610932005
>10	0.507944016812732	5.987521809990048
>50	0.02830860744460049	1.314095480479012
>100	0.01199777091789482	1.4616980908646822
>500	5.455687211635459E-4	0.2513080206691079
>1k	1.454849552367798E-4	0.12984410411939123
>5k	0.0	0.0
>10k+	3.637123880916658E-5	0.422061520798747
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17023	0.4219990852513514	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.47899362774688E-5	0.0
5	0.0	0.0	0.0	4.95798725549376E-5	0.0
6	0.0	0.0	0.0	4.95798725549376E-5	0.0
7	0.0	0.0	0.0	7.43698088324064E-5	0.0
8	0.0	0.0	0.0	1.23949681387344E-4	0.0
9	0.0	0.0	0.0	1.735295539422816E-4	0.0
10-11	0.0	0.0	0.0	1.85924522081016E-4	0.0
12-13	0.0	0.0	0.0	2.355043946359536E-4	0.0
14-15	0.0	0.0	0.0	2.726892990521568E-4	0.0
16-17	0.0	0.0	0.0	3.0987420346836E-4	0.0
18-19	0.0	0.0	0.0	3.222691716070944E-4	0.0
20-21	0.0	0.0	0.0	3.470591078845632E-4	0.0
22-23	0.0	0.0	0.0	5.205886618268447E-4	0.0
24-25	0.0	0.0	0.0	7.684880246015327E-4	0.0
26-27	0.0	0.0	0.0	0.001053572291792424	0.0
28-29	0.0	0.0	0.0	0.001983194902197504	0.0
30-31	0.0	0.0	0.0	0.0051439117775747755	0.0
32-33	0.0	0.0	0.0	0.010833202153253865	0.0
34-35	0.0	0.0	0.0	0.020637621950992772	0.0
36-37	0.0	0.0	0.0	0.035895827729774815	0.0
38-39	0.0	0.0	0.0	0.05738870248234027	0.0
40-41	0.0	0.0	0.0	0.08169523500239842	0.0
42-43	0.0	0.0	0.0	0.10655954108869964	0.0
44-45	0.0	0.0	0.0	0.13625788474910724	0.0
46-47	0.0	0.0	0.0	0.17207934267004965	0.0
48-49	0.0	0.0	0.0	0.2127720230695147	0.0
50-51	0.0	0.0	0.0	0.2550016795181828	0.0
52-53	0.0	0.0	0.0	0.297367680616377	0.0
54-55	0.0	0.0	0.0	0.34528662744072414	0.0
56-57	0.0	0.0	0.0	0.4042866757810999	0.0
58-59	0.0	0.0	0.0	0.4712938735390981	0.0
60-61	0.0	0.0	0.0	0.5424409906554335	0.0
62-63	0.0	0.0	0.0	0.6188683641988698	0.0
64-65	0.0	0.0	0.0	0.6990018332157877	0.0
66-67	0.0	0.0	0.0	0.7925218678225388	0.0
68-69	0.0	0.0	0.0	0.9008167044506612	0.0
70-71	0.0	0.0	0.0	1.0219651230386513	0.0
72-73	0.0	0.0	0.0	1.155161450657491	0.0
74-75	0.0	0.0	0.0	1.2890147115876838	0.0
76-77	0.0	0.0	0.0	1.4374072701446121	0.0
78-79	0.0	0.0	0.0	1.6100195964446273	0.0
80-81	0.0	0.0	0.0	1.804038032720237	0.0
82-83	0.0	0.0	0.0	2.0086169818500483	0.0
84-85	0.0	0.0	0.0	2.217707699382359	0.0
86-87	0.0	0.0	0.0	2.4435564138382384	0.0
88	0.0	0.0	0.0	2.631179046554261	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4750	0.0	48.933765	1
GTATCAA	8605	0.0	38.494358	1
TCAACGC	9720	0.0	32.959908	4
ATCAACG	9850	0.0	32.62326	3
TATCAAC	10635	0.0	30.746115	2
CAACGCA	10460	0.0	30.492832	5
AACGCAG	11255	0.0	28.476448	6
TACCTGG	3700	0.0	26.830185	2
GTACCTG	3935	0.0	26.66462	1
GTACATG	18400	0.0	26.620008	1
TACATGG	18435	0.0	25.521122	2
CGCAGAG	12560	0.0	25.181278	8
ACGCAGA	12740	0.0	24.936161	7
ACATGGG	18955	0.0	24.101183	3
CATGGGT	8855	0.0	22.525917	4
GCAGAGT	14195	0.0	21.453728	9
GTGGTAT	2520	0.0	19.418161	1
ACCTGGG	4865	0.0	19.24479	3
ATGGGTG	5360	0.0	18.43123	5
ATGGGTA	3765	0.0	18.117687	5
>>END_MODULE