FastQCFastQC Report
Wed 25 May 2016
SRR2431415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4033895
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230770.5720773594751475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191590.47495038914002474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123630.30647798219834677No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.12972573653999422No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41010.10166352867389955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102450.063.022371
GTATCAA205550.045.321381
ATCAACG261900.035.315663
TATCAAC264400.035.0368272
TCAACGC265250.034.8692054
CAACGCA275550.033.6169975
TATAACG4350.033.5113872
AACGCAG285350.032.5291186
GTGGTAT33350.030.316481
ACGCAGA317250.029.0804927
CGCAGAG318600.028.9130178
ATAACGC5650.028.2962533
TACCTGG34450.027.4363822
TGGTATC35350.027.4029792
GCAGAGT345750.026.2620149
GTACCTG37350.025.6847721
GTACATG177300.024.8258021
TACATGG180650.023.8439122
ACATGGG180250.023.1130333
GAGTACT237150.022.780812-13
Produced by FastQC (version 0.11.5)