##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431415_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4033895
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.630262066811355	34.0	31.0	34.0	31.0	34.0
2	32.72953609352747	34.0	31.0	34.0	31.0	34.0
3	32.826055710423795	34.0	31.0	34.0	31.0	34.0
4	36.303603589086975	37.0	37.0	37.0	35.0	37.0
5	36.212622787653125	37.0	37.0	37.0	35.0	37.0
6	36.25710287451706	37.0	37.0	37.0	35.0	37.0
7	36.20692729979338	37.0	37.0	37.0	35.0	37.0
8	36.217292963748434	37.0	37.0	37.0	35.0	37.0
9	38.00883017530204	39.0	39.0	39.0	35.0	39.0
10-11	37.98766551930578	39.0	38.5	39.0	35.0	39.0
12-13	37.97961040136146	39.0	39.0	39.0	35.0	39.0
14-15	39.44264637527749	41.0	39.0	41.0	36.0	41.0
16-17	39.47506925688448	41.0	39.0	41.0	36.0	41.0
18-19	39.54221961652448	41.0	39.5	41.0	36.5	41.0
20-21	39.53449990641799	41.0	40.0	41.0	36.0	41.0
22-23	39.477992486170315	41.0	39.0	41.0	36.0	41.0
24-25	39.43388945423716	41.0	39.0	41.0	36.0	41.0
26-27	39.32332336364729	41.0	39.0	41.0	36.0	41.0
28-29	39.23886479444805	41.0	39.0	41.0	35.5	41.0
30-31	39.06881512781071	41.0	39.0	41.0	35.0	41.0
32-33	38.85475725074649	41.0	38.0	41.0	35.0	41.0
34-35	38.72238494060951	41.0	38.0	41.0	35.0	41.0
36-37	38.580176851405405	41.0	38.0	41.0	35.0	41.0
38-39	38.43256083760237	41.0	38.0	41.0	35.0	41.0
40-41	38.29374525117783	40.5	38.0	41.0	34.5	41.0
42-43	38.12680944843631	40.0	38.0	41.0	34.0	41.0
44-45	37.896353400373584	40.0	38.0	41.0	33.5	41.0
46-47	37.54746144855035	40.0	38.0	41.0	33.0	41.0
48-49	37.22637153421197	40.0	38.0	41.0	32.0	41.0
50-51	37.06626746605948	40.0	37.0	41.0	31.5	41.0
52-53	37.007758506356765	40.0	37.0	41.0	32.0	41.0
54-55	36.87337498868959	40.0	37.0	41.0	31.0	41.0
56-57	36.73080360296934	40.0	36.5	41.0	31.0	41.0
58-59	36.558787474636794	40.0	36.0	41.0	31.0	41.0
60-61	36.40612683275097	40.0	36.0	41.0	31.0	41.0
62-63	36.248386485022536	39.0	35.0	41.0	31.0	41.0
64-65	36.015978105528276	39.0	35.0	41.0	30.5	41.0
66-67	35.75753087276689	39.0	35.0	41.0	30.0	41.0
68-69	35.46432901699227	38.0	35.0	41.0	30.0	41.0
70-71	35.1586249766045	37.0	35.0	40.0	29.0	41.0
72-73	34.8380040878605	37.0	35.0	40.0	29.0	41.0
74-75	34.50084484102834	36.5	35.0	39.0	29.0	41.0
76-77	34.164558076003466	36.0	35.0	39.0	29.0	41.0
78-79	33.837517957210096	35.5	35.0	38.5	29.0	40.0
80-81	33.56514336639898	35.0	34.0	37.0	29.0	39.0
82-83	33.354443658052574	35.0	34.0	37.0	29.0	39.0
84-85	33.081490346179066	35.0	34.0	36.5	29.0	39.0
86-87	32.83198583503041	35.0	34.0	36.0	29.0	37.0
88-89	32.59138239840155	35.0	34.0	36.0	29.0	37.0
90-91	32.403580534446235	35.0	34.0	35.5	28.5	37.0
92-93	32.235484934535975	35.0	34.0	35.0	27.5	36.0
94-95	32.09446911731713	35.0	34.0	35.0	27.0	36.0
96-97	31.948587407456067	35.0	34.0	35.0	27.0	36.0
98-99	31.82998293212887	35.0	34.0	35.0	27.0	36.0
100	31.727348133751622	35.0	34.0	35.0	26.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.009175341506718837
1101	2	0.03648396975148671
1101	3	0.0284469452024112
1101	4	0.0693680438680957
1101	5	0.06031378511966068
1101	6	0.026310149051717246
1101	7	0.03480068732376651
1101	8	0.0570152044084864
1101	9	0.06390140007105316
1101	10-11	0.06918243180480488
1101	12-13	0.07515637168840072
1101	14-15	0.07681911409153486
1101	16-17	0.012716135499331926
1101	18-19	0.03333335665423931
1101	20-21	0.03527596707127145
1101	22-23	0.04414316405217278
1101	24-25	0.0800594125756362
1101	26-27	0.09430773405596682
1101	28-29	0.06151663586424405
1101	30-31	0.02631833263755823
1101	32-33	7.828319428284658E-4
1101	34-35	0.06564114417383138
1101	36-37	0.04838850332175326
1101	38-39	0.005893910015451809
1101	40-41	0.015454074342898139
1101	42-43	-2.1589893534468274E-4
1101	44-45	-0.00206934736515052
1101	46-47	-0.016869245237415953
1101	48-49	0.05358544729720194
1101	50-51	-0.042464111749339395
1101	52-53	-0.05408752464055766
1101	54-55	-0.028963211879329265
1101	56-57	-0.005510204383298856
1101	58-59	-0.00975284242569785
1101	60-61	0.002033022512371474
1101	62-63	0.01120784578094458
1101	64-65	-0.03390003786107343
1101	66-67	-0.04247967582531942
1101	68-69	-0.04172949476717491
1101	70-71	-8.829729282879839E-4
1101	72-73	-0.017464407509635294
1101	74-75	-0.04210021229647509
1101	76-77	-0.08209300153340138
1101	78-79	-0.04093733354767437
1101	80-81	0.012692933960600783
1101	82-83	-0.02857395962409015
1101	84-85	0.051908265183421065
1101	86-87	0.06342229555525591
1101	88-89	0.01852516924525105
1101	90-91	-0.02791455381802166
1101	92-93	0.006942730345961934
1101	94-95	0.0015676340227415153
1101	96-97	0.01545346920877222
1101	98-99	-0.017280308926277854
1101	100	0.0014441019719733106
1102	1	-0.031175937331397563
1102	2	-0.025660751497333933
1102	3	0.007734282607010812
1102	4	0.039395511670328176
1102	5	-0.009396808294489745
1102	6	-0.004502471205562131
1102	7	0.0032271267254344593
1102	8	0.017933951124447844
1102	9	0.05182357636397228
1102	10-11	0.015277047113812614
1102	12-13	0.008430149316858149
1102	14-15	0.037120656267106256
1102	16-17	0.010545922778618433
1102	18-19	0.014371158077103985
1102	20-21	0.03988068092037622
1102	22-23	0.07928492824773059
1102	24-25	0.0650433479514092
1102	26-27	0.0798113273623855
1102	28-29	0.04553237019441525
1102	30-31	0.0178031013088642
1102	32-33	0.04311652486669715
1102	34-35	0.029174128217654527
1102	36-37	0.02273941524522627
1102	38-39	0.06497165717368603
1102	40-41	-0.031476949247377206
1102	42-43	0.012216612868307664
1102	44-45	-0.08076406327445085
1102	46-47	-0.14908790108707137
1102	48-49	-0.19490649333870635
1102	50-51	-0.21159566872435676
1102	52-53	-0.1656297545554608
1102	54-55	-0.14902752471647318
1102	56-57	-0.13361235195623067
1102	58-59	-0.11364804423111963
1102	60-61	-0.1907615168620822
1102	62-63	-0.1269902430024814
1102	64-65	-0.18349654988018926
1102	66-67	-0.14738799795983937
1102	68-69	-0.14429997640112902
1102	70-71	-0.2004834187071438
1102	72-73	-0.17801189236053006
1102	74-75	-0.13669171792707147
1102	76-77	-0.14009416252411455
1102	78-79	-0.12394223338210253
1102	80-81	-0.1481654714798495
1102	82-83	-0.12012253080694535
1102	84-85	-0.20689440821386995
1102	86-87	-0.1620795954845704
1102	88-89	-0.13118867631321507
1102	90-91	-0.1346068891529626
1102	92-93	-0.14942587310081734
1102	94-95	-0.14155010565073312
1102	96-97	-0.1289133277799266
1102	98-99	-0.10661002698084232
1102	100	-0.08708167608553907
1103	1	-0.03138002899312653
1103	2	-0.03509610458593926
1103	3	-0.03157768278065021
1103	4	0.029817745726859357
1103	5	0.02020199864683292
1103	6	0.018078491003883812
1103	7	-0.01025194911542826
1103	8	0.035415630512993346
1103	9	0.05351798846464817
1103	10-11	0.08240214276611368
1103	12-13	0.04859358311143325
1103	14-15	-0.011953860757742518
1103	16-17	0.051317118474941026
1103	18-19	-0.005258247569869923
1103	20-21	0.02240677406440028
1103	22-23	0.020610330589121872
1103	24-25	0.05928465349618506
1103	26-27	0.05392829244415509
1103	28-29	0.08764470384968348
1103	30-31	0.08562296108234335
1103	32-33	0.07220460119997796
1103	34-35	0.06759852053097148
1103	36-37	0.09967244409586584
1103	38-39	0.07446969197658149
1103	40-41	0.02510934441795598
1103	42-43	0.07054420455660448
1103	44-45	0.0784008558260183
1103	46-47	0.10228758645129687
1103	48-49	0.14663338075629895
1103	50-51	0.03220260246381912
1103	52-53	0.15940151108362244
1103	54-55	0.18242209773902118
1103	56-57	0.1847573518150014
1103	58-59	0.1998480308906565
1103	60-61	0.13908245080017423
1103	62-63	0.14827500093970514
1103	64-65	0.1542628646625701
1103	66-67	0.20534332013130552
1103	68-69	0.20884353664319377
1103	70-71	0.1721463349487138
1103	72-73	0.14731798896544035
1103	74-75	0.1113586300316669
1103	76-77	0.14899139651153348
1103	78-79	0.18809085052815533
1103	80-81	0.1936158525785956
1103	82-83	0.13164459208231705
1103	84-85	0.1212917534688458
1103	86-87	0.14072423281366753
1103	88-89	0.11314966052528064
1103	90-91	0.13730864397757614
1103	92-93	0.08709003652118241
1103	94-95	0.07597349526833597
1103	96-97	0.09438401120579343
1103	98-99	0.09518823618407168
1103	100	0.13073276798782985
1104	1	-0.10481969406130531
1104	2	-0.08048964348675014
1104	3	-0.08489091331919241
1104	4	-0.032475591895938294
1104	5	-0.024630147261959223
1104	6	-0.08307102271818678
1104	7	-0.07133558908209636
1104	8	-0.13314820542684913
1104	9	-0.05745644054543675
1104	10-11	-0.07567960468362855
1104	12-13	-0.09983476075918674
1104	14-15	-0.1009410469707035
1104	16-17	-0.08496606795760897
1104	18-19	-0.08824649300610332
1104	20-21	-0.043091507794486006
1104	22-23	-0.07451149957006464
1104	24-25	-0.02234052673141207
1104	26-27	-0.08188968828686427
1104	28-29	-0.07424113753817352
1104	30-31	-0.029606403443587226
1104	32-33	0.06489814715491349
1104	34-35	0.03714713055774865
1104	36-37	0.07616336018326564
1104	38-39	0.07106221068730889
1104	40-41	0.04542043353897185
1104	42-43	0.04132506158769189
1104	44-45	0.08382925883960013
1104	46-47	0.05042857806084555
1104	48-49	0.10742028235024748
1104	50-51	0.0590530897885273
1104	52-53	0.09481859319041064
1104	54-55	0.15728618957543716
1104	56-57	0.1229427553180642
1104	58-59	0.14795241433856177
1104	60-61	0.1333004257390229
1104	62-63	0.07722671532695813
1104	64-65	0.1636042847068282
1104	66-67	0.12702417093406382
1104	68-69	-0.017441367668773466
1104	70-71	0.025520848538477026
1104	72-73	0.07658963949456421
1104	74-75	0.060200076216297305
1104	76-77	0.026879319592403306
1104	78-79	0.026283691972246004
1104	80-81	0.06199908728506642
1104	82-83	-0.0010139211122393021
1104	84-85	-2.0744790155902137E-5
1104	86-87	0.01872971490768549
1104	88-89	0.06525772363134763
1104	90-91	0.06036763650352839
1104	92-93	0.006000079829298954
1104	94-95	0.008814223479397754
1104	96-97	0.04725735644564821
1104	98-99	0.023629500787574642
1104	100	0.041440315067116984
1105	1	-0.17143668689775637
1105	2	-0.17825473236246836
1105	3	-0.15328041714805352
1105	4	-0.04908548235135157
1105	5	-0.11057041426693814
1105	6	-0.05340457417772626
1105	7	-0.04343308412627778
1105	8	-0.07005772897445439
1105	9	-0.06896178378063667
1105	10-11	-0.1115002792436286
1105	12-13	-0.15825398663906043
1105	14-15	-0.1438618322044647
1105	16-17	-0.19069820349879762
1105	18-19	-0.22285961714389657
1105	20-21	-0.10043590953326031
1105	22-23	-0.11352074911482646
1105	24-25	-0.1479550026348022
1105	26-27	-0.07112298411208684
1105	28-29	-0.13835914604081267
1105	30-31	-0.06896794479563795
1105	32-33	-0.14777661297051736
1105	34-35	-0.13659612924838171
1105	36-37	-0.07188285961083096
1105	38-39	-0.0872678970170071
1105	40-41	-0.12611847508637197
1105	42-43	-0.1482694018792543
1105	44-45	-0.10563155306621752
1105	46-47	-0.04090028856877126
1105	48-49	-0.0345528019388226
1105	50-51	-0.03748030570992711
1105	52-53	-0.03316387138207233
1105	54-55	0.01871393779850905
1105	56-57	-0.0022289997890965196
1105	58-59	0.017513074241428228
1105	60-61	0.007381549837752743
1105	62-63	-0.03291112398308371
1105	64-65	0.006081698021368709
1105	66-67	-0.020719876388184844
1105	68-69	0.08915243490833547
1105	70-71	0.07462486046704697
1105	72-73	0.135682092276582
1105	74-75	0.09536680054161906
1105	76-77	0.031798168249316916
1105	78-79	0.1129488901852227
1105	80-81	0.13907118767588855
1105	82-83	0.07584348004000674
1105	84-85	0.03297105740005435
1105	86-87	-0.01135143900562241
1105	88-89	0.01767965269972649
1105	90-91	0.04388844185356788
1105	92-93	0.042598075295778415
1105	94-95	0.03157906818399425
1105	96-97	-0.0018214673041008211
1105	98-99	-0.030999075526864317
1105	100	-0.01415565009506281
1106	1	-0.10268493329113682
1106	2	-0.062330710449423066
1106	3	-0.09456769290056855
1106	4	-0.06467439156965327
1106	5	-0.052912857788285805
1106	6	-0.0871716671835685
1106	7	-0.061204364427652536
1106	8	-0.06344928816631068
1106	9	-0.09578901684360375
1106	10-11	-0.09095346103419644
1106	12-13	-0.0688903325155863
1106	14-15	-0.06708469304915354
1106	16-17	-0.11604398524188753
1106	18-19	-0.09026103524843876
1106	20-21	-0.08739673877308007
1106	22-23	-0.1175625550965762
1106	24-25	-0.05659202837112787
1106	26-27	-0.09647084626397628
1106	28-29	-0.11750673302248771
1106	30-31	-0.17290966030740407
1106	32-33	-0.11945688128317755
1106	34-35	-0.10718809066002422
1106	36-37	-0.11020262689875437
1106	38-39	-0.09101661011171558
1106	40-41	-0.14635541529230522
1106	42-43	-0.08686663673783102
1106	44-45	-0.03180497872519794
1106	46-47	-0.09827826925501171
1106	48-49	-0.02108666064769693
1106	50-51	-0.10206182702943778
1106	52-53	-0.13982871129406504
1106	54-55	-0.10482998463761817
1106	56-57	-0.11818646677679112
1106	58-59	-0.14243389980952514
1106	60-61	-0.12824226074135936
1106	62-63	-0.2019490951150189
1106	64-65	-0.15982546646153395
1106	66-67	-0.21831553291736583
1106	68-69	-0.20155464319866212
1106	70-71	-0.11875513131811744
1106	72-73	-0.13955565839876982
1106	74-75	-0.1415790236106531
1106	76-77	-0.18193200417541533
1106	78-79	-0.14331525409146195
1106	80-81	-0.14192168853711706
1106	82-83	-0.14004841235101395
1106	84-85	-0.13611240181470663
1106	86-87	-0.1430213186244913
1106	88-89	-0.13622679320775433
1106	90-91	-0.15103636471847892
1106	92-93	-0.11567156915902643
1106	94-95	-0.20189873118405188
1106	96-97	-0.21405667965820996
1106	98-99	-0.1767550193215648
1106	100	-0.10483366088604029
1107	1	-0.3001261411823606
1107	2	-0.22885177994891848
1107	3	-0.22272417754791007
1107	4	-0.27167107277399083
1107	5	-0.3090701560673992
1107	6	-0.27048512242759415
1107	7	-0.1841709954696924
1107	8	-0.18209068597781197
1107	9	-0.19591520955628994
1107	10-11	-0.16249233993181633
1107	12-13	-0.15529654484112854
1107	14-15	-0.2170300237493592
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2216	32-33	-0.08221725025746451
2216	34-35	-0.02366096917319993
2216	36-37	-0.03670221577298349
2216	38-39	-0.036371800695270906
2216	40-41	-0.056870001139529336
2216	42-43	0.006277744993475665
2216	44-45	-0.017675142526925924
2216	46-47	0.06504085029121143
2216	48-49	0.13764139843455325
2216	50-51	0.13134091683831883
2216	52-53	0.0615552478421435
2216	54-55	0.0983727575347757
2216	56-57	0.07161592622948376
2216	58-59	0.07004806477036851
2216	60-61	0.043606449411917936
2216	62-63	-0.011278431792035803
2216	64-65	-0.005951015163134343
2216	66-67	0.012821559044496667
2216	68-69	0.045957003557660414
2216	70-71	0.030221664767246637
2216	72-73	0.05493580830201239
2216	74-75	0.060407578282585916
2216	76-77	0.08355158509545646
2216	78-79	0.10498652345825121
2216	80-81	0.1035806315487875
2216	82-83	0.1222467467736763
2216	84-85	0.17034219633770675
2216	86-87	0.17529561567640428
2216	88-89	0.18920317869849157
2216	90-91	0.16695488966752947
2216	92-93	0.1593667268464003
2216	94-95	0.0919853960100383
2216	96-97	0.06657546736059672
2216	98-99	0.078732735912034
2216	100	0.11095226747458042
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45.0
3	213.0
4	65.0
5	112.0
6	308.0
7	638.0
8	1252.0
9	2226.0
10	3497.0
11	5311.0
12	8339.0
13	12362.0
14	13692.0
15	17398.0
16	22054.0
17	33285.0
18	38302.0
19	19067.0
20	11742.0
21	10467.0
22	10248.0
23	10696.0
24	11441.0
25	13789.0
26	16662.0
27	21192.0
28	26492.0
29	33654.0
30	43029.0
31	54473.0
32	69205.0
33	90858.0
34	125392.0
35	196582.0
36	318722.0
37	673319.0
38	1264561.0
39	844294.0
40	8911.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.316012593896424	21.17730619530456	14.874953516622455	22.63172769417656
2	14.333805354665524	22.042776437409078	40.992474433004205	22.63094377492119
3	16.16366573323391	28.50106696917292	32.10930186898094	23.225965428612227
4	10.975992186212085	18.833336018909762	40.176033337506304	30.014638457371845
5	11.121962736653202	38.943634761068864	35.90127489790847	14.033127604369463
6	26.92759727261121	40.410868403862764	18.92022474556229	13.741309577963731
7	23.835474150913697	34.894487833719026	24.183946285166076	17.086091730201208
8	24.39438805422551	35.371223098271024	21.731180410000757	18.50320843750271
9	25.338320739216254	16.06574193570778	22.697795232100795	35.89814209297518
10-11	22.30339411412543	27.643295127909873	30.216899547459715	19.836411210504984
12-13	24.140848980947695	25.929963472028895	30.138823643153824	19.790363903869583
14-15	21.41707243670756	27.043893567852802	28.892194489989077	22.646839505450565
16-17	19.186233156787672	30.336263090635722	29.29452799341579	21.18297575916081
18-19	19.115591315882696	27.99010781892331	33.24068090055933	19.653619964634657
20-21	20.744838425392828	27.140852699438135	32.95255578045537	19.16175309471367
22-23	20.21270516933578	27.307525595308853	33.00760171283649	19.472167522518877
24-25	19.916413272138534	27.326548491358476	33.02972264516618	19.727315591336808
26-27	19.713559439261	27.223653291573818	33.12503981887463	19.937747450290548
28-29	19.707661871337358	27.282297325621506	33.14682190803146	19.86321889500967
30-31	19.970326391105353	27.725426155290645	32.77706678731125	19.52718066629275
32-33	19.586552917361775	27.464936339219083	33.1267106057588	19.821800137660343
34-35	19.816116823924844	27.290572776905925	32.90910418307204	19.98420621609719
36-37	20.19870106673565	27.570296703012026	32.33586982753566	19.895132402716662
38-39	20.064246243884106	27.655967759868382	32.389740383546304	19.8900456127012
40-41	19.959280005245557	27.550041331067366	32.41052279430986	20.08015586937722
42-43	19.929655780368172	27.625599980961248	32.19067359002924	20.25407064864133
44-45	20.28397895282482	27.75495517292797	31.778700983579007	20.182364890668204
46-47	20.408816405210953	28.16915636498674	31.122894060793442	20.299133169008865
48-49	20.462469229453458	28.09591981933072	30.750156796541305	20.69145415467452
50-51	20.486131855518934	28.160073973353235	30.540384765554794	20.81340940557304
52-53	20.517906607891074	28.320170634977693	30.39557011448548	20.766352642645757
54-55	20.647544899224492	28.646209703988752	30.226839407447	20.479405989339757
56-57	20.439603318790866	28.26973994987628	30.318567878054008	20.972088853278848
58-59	20.319089888417572	27.965885937580182	30.711183949741677	21.00384022426057
60-61	20.570717729871248	28.283100724729387	30.341407050162122	20.804774495237243
62-63	20.4526413997725	28.171423096340458	30.53232819073465	20.843607313152393
64-65	20.40176610893914	27.956218882779048	30.79470563962696	20.84730936865485
66-67	20.378060094710246	28.104524611215126	30.43136482036847	21.086050473706162
68-69	20.844438731729316	28.216758205765085	30.149224575356794	20.78957848714881
70-71	20.823892993492255	28.02295702245391	30.167936100318165	20.985213883735668
72-73	20.657662869133407	28.141889324958125	30.32769481472043	20.872752991188033
74-75	20.69179438514658	28.25323907154218	30.095959911469677	20.959006631841564
76-77	20.739558527725016	28.319954108540045	29.903236746089235	21.037250617645704
78-79	20.82620307134873	28.304258225923885	30.05053716499544	20.819001537731946
80-81	20.698445389827594	28.19496300453683	30.0728357090072	21.033755896628374
82-83	20.529255527492936	28.20669952868684	30.242553242124863	21.021491701695357
84-85	20.574112150042417	28.20774516429234	29.969884819598963	21.248257866066282
86-87	20.66675525104126	28.286556734449412	29.893941696442006	21.15274631806732
88-89	20.481658901340047	28.101859501238337	30.00812747026693	21.408354127154688
90-91	20.38950358249341	28.09920541219591	29.94812031426533	21.563170691045347
92-93	20.45329862045047	28.22438001583102	29.853356084121064	21.468965279597445
94-95	20.28478890592521	28.06025982525444	30.046431895960822	21.60851937285953
96-97	20.239081819513714	28.08039433886538	29.977371723727785	21.703152117893122
98-99	20.238876714230468	27.982537903953514	29.957609051335194	21.820976330480825
100	20.277454337489207	28.08629587973293	29.79115191320336	21.8450978695745
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2608.0
1	1498.5
2	2202.5
3	3771.0
4	2243.5
5	1188.5
6	2409.0
7	6326.0
8	11033.0
9	12247.0
10	8472.0
11	4604.0
12	4013.0
13	4347.5
14	4902.5
15	5458.5
16	6081.0
17	6746.5
18	7537.5
19	8550.5
20	10008.5
21	12265.0
22	15719.0
23	21390.0
24	27953.5
25	34884.0
26	44491.5
27	55969.5
28	70533.5
29	85113.0
30	94914.5
31	104634.5
32	114737.5
33	124035.5
34	132088.5
35	137638.5
36	140964.5
37	142740.5
38	142334.0
39	140779.0
40	139880.0
41	136814.0
42	131028.5
43	126081.0
44	122284.5
45	118957.0
46	116929.5
47	113615.0
48	109219.0
49	106780.0
50	104855.0
51	99851.5
52	93668.5
53	88683.5
54	84516.0
55	80574.5
56	74026.5
57	66676.0
58	61420.0
59	57059.0
60	50279.5
61	41633.5
62	34991.5
63	28462.5
64	21915.0
65	16991.5
66	12920.0
67	9677.0
68	7249.0
69	5299.5
70	3773.0
71	2729.0
72	2059.5
73	1491.0
74	1113.5
75	824.5
76	510.5
77	372.0
78	265.0
79	136.5
80	72.0
81	35.5
82	17.5
83	6.0
84	3.5
85	3.5
86	2.0
87	3.0
88	2.5
89	1.0
90	1.5
91	1.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004834037574106416
2	0.004437398593666915
3	9.668075148212832E-4
4	0.0
5	0.04286179982374355
6	0.0
7	0.0
8	0.0
9	2.4789936277468796E-5
10-11	0.0
12-13	0.0
14-15	2.4789936277468796E-5
16-17	0.0
18-19	4.957987255493759E-5
20-21	0.0
22-23	3.71849044162032E-5
24-25	2.4789936277468796E-5
26-27	9.915974510987518E-5
28-29	1.115547132486096E-4
30-31	1.85924522081016E-4
32-33	0.0013262615908445807
34-35	7.313031201853296E-4
36-37	0.0011527320369022992
38-39	0.0010039924192374863
40-41	1.115547132486096E-4
42-43	4.214289167169696E-4
44-45	1.115547132486096E-4
46-47	1.487396176648128E-4
48-49	1.23949681387344E-4
50-51	2.47899362774688E-4
52-53	5.205886618268447E-4
54-55	6.941182157691264E-4
56-57	0.001896430125226363
58-59	3.9663898043950074E-4
60-61	0.0011031521643473615
62-63	9.792024829600174E-4
64-65	0.0013758414633995182
66-67	3.0987420346836E-4
68-69	2.107144583584848E-4
70-71	2.3550439463595357E-4
72-73	1.115547132486096E-4
74-75	5.701685343817823E-4
76-77	7.189081520465951E-4
78-79	7.932779608790015E-4
80-81	5.453785981043136E-4
82-83	0.0014626062403706591
84-85	0.0012147068775959712
86-87	0.0018840351570876285
88-89	0.0011031521643473615
90-91	0.001053572291792424
92-93	0.0010411773236536895
94-95	7.43698088324064E-4
96-97	9.915974510987518E-5
98-99	3.71849044162032E-4
100	1.487396176648128E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4033895.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.276755130904846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2092610811816	48.22274752141125
2	15.362204720867103	19.441409327862765
3	4.337693660472545	8.234255387597987
4	1.6226087640089413	4.106936697338157
5	0.7707360644110127	2.4384838609146477
6	0.44474931490089537	1.688537609617299
7	0.2634739128289135	1.1670241985819572
8	0.1701434137044914	0.8612898500892273
9	0.14422090480195798	0.8213247790119931
>10	0.6193140290051574	6.799534695083115
>50	0.03588897852649178	1.5661600611374684
>100	0.017899949512913994	2.026949554278817
>500	0.0010595774058764166	0.43913050859000025
>1k	5.886539778891364E-4	0.7004631925815308
>5k	3.9243598525938674E-5	0.12994625236737656
>10k+	1.1773079557781601E-4	1.3558065035364786
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23077	0.5720773594751475	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19159	0.47495038914002474	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12363	0.30647798219834677	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5233	0.12972573653999422	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4101	0.10166352867389955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.47899362774688E-5	0.0
3	0.0	0.0	0.0	4.95798725549376E-5	0.0
4	0.0	0.0	0.0	7.43698088324064E-5	0.0
5	0.0	0.0	0.0	7.43698088324064E-5	0.0
6	0.0	0.0	0.0	7.43698088324064E-5	0.0
7	0.0	0.0	0.0	9.91597451098752E-5	0.0
8	0.0	0.0	0.0	1.23949681387344E-4	0.0
9	0.0	0.0	0.0	2.47899362774688E-4	0.0
10-11	0.0	0.0	0.0	2.602943309134224E-4	0.0
12-13	0.0	0.0	0.0	3.594540760232976E-4	0.0
14-15	0.0	0.0	0.0	4.95798725549376E-4	0.0
16-17	0.0	0.0	0.0	5.081936936881103E-4	0.0
18-19	0.0	0.0	0.0	5.205886618268447E-4	0.0
20-21	0.0	0.0	0.0	5.453785981043135E-4	0.0
22-23	0.0	0.0	0.0	7.189081520465951E-4	0.0
24-25	0.0	0.0	0.0	0.0010411773236536897	0.0
26-27	0.0	0.0	0.0	0.0013510515271220496	0.0
28-29	0.0	0.0	0.0	0.0023426489782208014	0.0
30-31	0.0	0.0	0.0	0.005528155789875542	0.0
32-33	0.0	0.0	0.0	0.011279421006248302	0.0
34-35	0.0	0.0	0.0	0.02135653010303937	0.0
36-37	0.0	0.0	0.0	0.0367510805313475	0.0
38-39	0.0	0.0	0.0	0.05870256910504612	0.0
40-41	0.0	0.0	0.0	0.08331897582857264	0.0
42-43	0.0	0.0	0.0	0.10869147560856195	0.0
44-45	0.0	0.0	0.0	0.1387988532175478	0.0
46-47	0.0	0.0	0.0	0.17495497527823606	0.0
48-49	0.0	0.0	0.0	0.21654009338368996	0.0
50-51	0.0	0.0	0.0	0.259612607665792	0.0
52-53	0.0	0.0	0.0	0.3027718867248652	0.0
54-55	0.0	0.0	0.0	0.3516700360321724	0.0
56-57	0.0	0.0	0.0	0.41179802647317293	0.0
58-59	0.0	0.0	0.0	0.4799951411724896	0.0
60-61	0.0	0.0	0.0	0.5525180997522245	0.0
62-63	0.0	0.0	0.0	0.630296524822783	0.0
64-65	0.0	0.0	0.0	0.711966969888904	0.0
66-67	0.0	0.0	0.0	0.8068504509909158	0.0
68-69	0.0	0.0	0.0	0.917066507680542	0.0
70-71	0.0	0.0	0.0	1.0410781639085798	0.0
72-73	0.0	0.0	0.0	1.177150124135606	0.0
74-75	0.0	0.0	0.0	1.3140649421960662	0.0
76-77	0.0	0.0	0.0	1.4656182176283714	0.0
78-79	0.0	0.0	0.0	1.6419366394018686	0.0
80-81	0.0	0.0	0.0	1.8403181044623125	0.0
82-83	0.0	0.0	0.0	2.0498426458794787	0.0
84-85	0.0	0.0	0.0	2.2635938714319535	0.0
86-87	0.0	0.0	0.0	2.495454145435119	0.0
88	0.0	0.0	0.0	2.6875761515855023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10245	0.0	63.02237	1
GTATCAA	20555	0.0	45.32138	1
ATCAACG	26190	0.0	35.31566	3
TATCAAC	26440	0.0	35.036827	2
TCAACGC	26525	0.0	34.869205	4
CAACGCA	27555	0.0	33.616997	5
TATAACG	435	0.0	33.511387	2
AACGCAG	28535	0.0	32.529118	6
GTGGTAT	3335	0.0	30.31648	1
ACGCAGA	31725	0.0	29.080492	7
CGCAGAG	31860	0.0	28.913017	8
ATAACGC	565	0.0	28.296253	3
TACCTGG	3445	0.0	27.436382	2
TGGTATC	3535	0.0	27.402979	2
GCAGAGT	34575	0.0	26.262014	9
GTACCTG	3735	0.0	25.684772	1
GTACATG	17730	0.0	24.825802	1
TACATGG	18065	0.0	23.843912	2
ACATGGG	18025	0.0	23.113033	3
GAGTACT	23715	0.0	22.7808	12-13
>>END_MODULE