FastQCFastQC Report
Wed 25 May 2016
SRR2431414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853193
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA201390.5226574428013339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50450.049.146771
GTATCAA91750.039.0745931
TCAACGC104450.033.1485184
ATCAACG105200.033.003343
CAACGCA112400.030.8872415
TATCAAC116350.030.5279672
AACGCAG124500.028.0465666
GTACATG187950.026.5594221
TACATGG187850.025.9958152
CATGGGT89600.025.674124
CGCAGAG132850.025.5759248
ACATGGG193750.024.7671383
ACGCAGA138800.024.717527
TACCTGG29500.022.4861152
ATGGGTG49850.022.459545
GCAGAGT149350.021.7122149
GTACCTG32200.021.6247651
GTGGTAT27200.019.0269571
TGGTATC25100.018.9306262
CAGAGTA146650.018.73095310-11
Produced by FastQC (version 0.11.5)