##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431414_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3853193
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.751222427737204	31.0	31.0	34.0	27.0	34.0
2	30.863537331247098	33.0	31.0	34.0	27.0	34.0
3	30.9450637432384	34.0	31.0	34.0	27.0	34.0
4	34.18889087569712	37.0	35.0	37.0	30.0	37.0
5	34.297343268297226	37.0	35.0	37.0	31.0	37.0
6	34.43571422454053	37.0	35.0	37.0	31.0	37.0
7	34.39058801362921	37.0	35.0	37.0	31.0	37.0
8	34.36198835615034	37.0	35.0	37.0	31.0	37.0
9	35.849117861472294	39.0	37.0	39.0	30.0	39.0
10-11	35.86408324213191	39.0	37.0	39.0	30.5	39.0
12-13	35.79818568652024	39.0	37.0	39.0	30.0	39.0
14-15	37.167616441740655	41.0	38.0	41.0	30.5	41.0
16-17	37.19116561251928	41.0	38.0	41.0	31.0	41.0
18-19	37.22162009533392	41.0	38.0	41.0	31.0	41.0
20-21	37.30935603277594	41.0	38.0	41.0	31.0	41.0
22-23	37.290066575954015	41.0	38.0	41.0	30.0	41.0
24-25	37.34154557012846	41.0	38.0	41.0	30.0	41.0
26-27	37.38431594264809	41.0	38.0	41.0	30.0	41.0
28-29	37.35742292171713	41.0	38.0	41.0	30.0	41.0
30-31	37.29332478284893	40.0	38.0	41.0	30.0	41.0
32-33	37.242109595859844	40.0	38.0	41.0	30.0	41.0
34-35	37.169687840707695	40.0	38.0	41.0	30.0	41.0
36-37	37.042794378584205	40.0	38.0	41.0	30.0	41.0
38-39	36.92198638895067	40.0	38.0	41.0	30.0	41.0
40-41	36.86463590066732	40.0	37.5	41.0	30.0	41.0
42-43	36.70530972624522	40.0	37.0	41.0	29.5	41.0
44-45	36.558550531987365	40.0	37.0	41.0	29.0	41.0
46-47	36.437193127881216	40.0	37.0	41.0	29.0	41.0
48-49	36.30906562427576	40.0	36.0	41.0	29.0	41.0
50-51	36.180923068219	40.0	36.0	41.0	29.0	41.0
52-53	36.02766900074821	40.0	35.5	41.0	28.0	41.0
54-55	35.87327613747871	40.0	35.0	41.0	28.0	41.0
56-57	35.68947532604777	39.5	35.0	41.0	28.0	41.0
58-59	35.483145796226665	39.0	35.0	41.0	28.0	41.0
60-61	35.25313214261523	39.0	35.0	41.0	27.0	41.0
62-63	35.151742204452255	39.0	35.0	41.0	27.5	41.0
64-65	34.89592034450389	38.5	34.5	41.0	26.5	41.0
66-67	34.62849200649954	38.0	34.0	40.0	26.0	41.0
68-69	34.32186526862267	37.0	34.0	40.0	26.0	41.0
70-71	33.98558053022519	37.0	34.0	40.0	26.0	41.0
72-73	33.63190761532059	36.0	34.0	39.0	25.0	41.0
74-75	33.33887440883444	36.0	33.5	39.0	25.0	41.0
76-77	33.06207280559266	35.5	33.0	39.0	25.0	40.5
78-79	32.70545428168275	35.0	33.0	37.5	24.0	39.5
80-81	32.37274670643282	35.0	33.0	37.0	24.0	39.0
82-83	32.047853689135216	35.0	33.0	37.0	23.0	39.0
84-85	31.745734900899073	35.0	33.0	36.0	22.5	38.0
86-87	31.463726966181035	35.0	33.0	36.0	21.5	37.0
88-89	31.19734152947958	35.0	32.0	35.5	20.0	37.0
90-91	30.97205447534032	35.0	32.0	35.0	19.5	36.5
92-93	30.745037297638607	35.0	32.0	35.0	18.0	36.0
94-95	30.540068846797965	35.0	32.0	35.0	13.0	36.0
96-97	30.34953400984586	35.0	32.0	35.0	2.0	36.0
98-99	30.145102256751738	35.0	32.0	35.0	2.0	35.5
100	29.9804580253312	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.05876450644769449
1101	2	0.13252920262289436
1101	3	0.14792434886141237
1101	4	0.18508055849865457
1101	5	0.1662619938039569
1101	6	0.19517598361042587
1101	7	0.2022850109523162
1101	8	0.16268585123810908
1101	9	0.14263511602589318
1101	10-11	0.10989439502614573
1101	12-13	0.07177924566485672
1101	14-15	0.15904004530099058
1101	16-17	0.14578974174443005
1101	18-19	0.015521916972517147
1101	20-21	0.034110036126726584
1101	22-23	0.0630263245669127
1101	24-25	0.06599636009281085
1101	26-27	0.06543704085910917
1101	28-29	0.08587861913784423
1101	30-31	0.10517619922559618
1101	32-33	0.047210606730381244
1101	34-35	0.10645717859176074
1101	36-37	0.06035991360867854
1101	38-39	0.16683407598846145
1101	40-41	0.0645449762903354
1101	42-43	0.11237377431407936
1101	44-45	0.06656458750256888
1101	46-47	0.04061972555699356
1101	48-49	0.042865984083796604
1101	50-51	0.0019984767549701132
1101	52-53	0.02431792880024375
1101	54-55	-0.009828418578877063
1101	56-57	-0.0386464481576283
1101	58-59	0.001263208983765196
1101	60-61	-0.06660192343553462
1101	62-63	-0.09677321428083019
1101	64-65	-0.08327823741512219
1101	66-67	-0.036182006669861266
1101	68-69	-0.02480502415375696
1101	70-71	-0.014945539053336176
1101	72-73	-0.022807020520730248
1101	74-75	-0.03317890808546764
1101	76-77	-0.053248603038539954
1101	78-79	-0.02600396804511007
1101	80-81	-0.0631771565597532
1101	82-83	-0.030358130065803124
1101	84-85	-0.05672206963211934
1101	86-87	-0.02236270114512351
1101	88-89	-0.05537381565105548
1101	90-91	-0.01630015100159099
1101	92-93	0.003120519579113079
1101	94-95	-0.0144029541003583
1101	96-97	0.005468056846737568
1101	98-99	0.0013210569998030053
1101	100	0.048351896326760624
1102	1	0.029112196030059323
1102	2	0.10278776079978158
1102	3	0.07776065149131384
1102	4	0.1563363448948749
1102	5	0.15255938615535314
1102	6	0.14901355920429182
1102	7	0.06714976983025878
1102	8	0.10648477796571143
1102	9	0.18048502325877536
1102	10-11	0.17308351006760603
1102	12-13	0.11117968043469517
1102	14-15	0.18594101346346292
1102	16-17	0.06783648461966862
1102	18-19	0.08923438690146668
1102	20-21	0.10544086638078198
1102	22-23	0.05509856460728457
1102	24-25	0.08092314538954781
1102	26-27	0.07850108945081757
1102	28-29	0.02356938593512581
1102	30-31	0.10940075200993249
1102	32-33	0.10139055393214846
1102	34-35	0.09814366442292766
1102	36-37	0.06142543602571493
1102	38-39	0.13199308482064964
1102	40-41	0.08441682646556359
1102	42-43	0.06565034049112484
1102	44-45	-0.011818208554274179
1102	46-47	-0.013828214058570154
1102	48-49	-0.018376098275744823
1102	50-51	-0.11902204859258347
1102	52-53	-0.056898594580438555
1102	54-55	-0.08826621555893155
1102	56-57	-0.13781849649569722
1102	58-59	-0.13720478898662236
1102	60-61	-0.1624880903344561
1102	62-63	-0.04624923328010766
1102	64-65	-0.028973566065417344
1102	66-67	-0.049167275993596604
1102	68-69	-0.027536426497171362
1102	70-71	-0.09478659070555295
1102	72-73	-0.09242323438080291
1102	74-75	-0.07031744235041515
1102	76-77	-0.06196252725172258
1102	78-79	-0.0852638570117108
1102	80-81	-0.07272812503462234
1102	82-83	-0.1452849685356341
1102	84-85	-0.17723931160733386
1102	86-87	-0.20146857157477882
1102	88-89	-0.2487334661230136
1102	90-91	-0.26946222220541927
1102	92-93	-0.22383573863529804
1102	94-95	-0.22232096676645696
1102	96-97	-0.2927763934437948
1102	98-99	-0.39963562340900793
1102	100	-0.4007538385114948
1103	1	-0.25465276636573364
1103	2	-0.2577061910237717
1103	3	-0.23153137553853753
1103	4	-0.06483638581890006
1103	5	-0.08594403965896902
1103	6	-0.044384689098009744
1103	7	-0.08911225715462479
1103	8	-0.09979952084602672
1103	9	0.010399022794821633
1103	10-11	0.032034817161978424
1103	12-13	-0.013836388609028916
1103	14-15	-0.03067450669345817
1103	16-17	-0.09469086581854214
1103	18-19	-0.06467972465499372
1103	20-21	-0.18005902371488958
1103	22-23	-0.09940110819259473
1103	24-25	-0.05574423677939677
1103	26-27	-0.025353867525140572
1103	28-29	-0.04545510870998726
1103	30-31	-6.283547813623613E-4
1103	32-33	-0.03919372377160357
1103	34-35	-0.05773485466458084
1103	36-37	-0.16180332221941995
1103	38-39	-0.1424242900081154
1103	40-41	-0.157019100636262
1103	42-43	-0.21668285688354416
1103	44-45	-0.252134906922592
1103	46-47	-0.3508288933228414
1103	48-49	-0.3116122079430639
1103	50-51	-0.4116443108705923
1103	52-53	-0.4669935017913005
1103	54-55	-0.46394314882360277
1103	56-57	-0.46175325776525966
1103	58-59	-0.43701763422308204
1103	60-61	-0.40931103208834685
1103	62-63	-0.33062933255637716
1103	64-65	-0.35149678087401526
1103	66-67	-0.3644146030437412
1103	68-69	-0.4137469320279692
1103	70-71	-0.39500235233457914
1103	72-73	-0.35449887275828473
1103	74-75	-0.40042757679871244
1103	76-77	-0.44963910157495235
1103	78-79	-0.42256250792621586
1103	80-81	-0.4066173282507606
1103	82-83	-0.4429225248401103
1103	84-85	-0.4780403203729655
1103	86-87	-0.5090249434994725
1103	88-89	-0.5159354104905276
1103	90-91	-0.5900120691725768
1103	92-93	-0.6198511382016605
1103	94-95	-0.6034019095154299
1103	96-97	-0.6336133419813237
1103	98-99	-0.64215996371194
1103	100	-0.5898109652183514
1104	1	-0.19523686688433273
1104	2	-0.20374014251623862
1104	3	-0.10906608003830698
1104	4	-0.025709149639261852
1104	5	-0.057154900607820025
1104	6	-0.022147343716561352
1104	7	-0.07907110955888896
1104	8	-0.08346887187345686
1104	9	-0.030592615358386865
1104	10-11	-0.01914880634696914
1104	12-13	-0.04774062120321787
1104	14-15	-0.09256406667254424
1104	16-17	-0.10392544492211897
1104	18-19	-0.08955889691408458
1104	20-21	-0.11884527991381333
1104	22-23	-0.07538127795005778
1104	24-25	-0.1031218652159751
1104	26-27	-0.11645199792458527
1104	28-29	-0.029004734726854053
1104	30-31	-0.09023543620067187
1104	32-33	-0.1439661044719358
1104	34-35	-0.10171095377168626
1104	36-37	-0.1976326069570007
1104	38-39	-0.16062406694151576
1104	40-41	-0.09225535273932195
1104	42-43	-0.21691167343332296
1104	44-45	-0.21262722876862483
1104	46-47	-0.2600690648446715
1104	48-49	-0.2960597715390989
1104	50-51	-0.3372675110571066
1104	52-53	-0.39596151946018665
1104	54-55	-0.2877473165142348
1104	56-57	-0.3178158329872147
1104	58-59	-0.31813069874225874
1104	60-61	-0.3604860700537742
1104	62-63	-0.3035009841944003
1104	64-65	-0.2651982843680898
1104	66-67	-0.17558266230131636
1104	68-69	-0.19718880553534035
1104	70-71	-0.2649211283668933
1104	72-73	-0.33634005438112524
1104	74-75	-0.226169399265423
1104	76-77	-0.24098518877256225
1104	78-79	-0.2845132694402963
1104	80-81	-0.30593371419378457
1104	82-83	-0.296208582547564
1104	84-85	-0.3302413701722706
1104	86-87	-0.3331032184511109
1104	88-89	-0.3479986347480768
1104	90-91	-0.37396592463298006
1104	92-93	-0.4722116900709459
1104	94-95	-0.43708969189316704
1104	96-97	-0.5288917656440262
1104	98-99	-0.5259506938454663
1104	100	-0.45975258352241255
1105	1	-0.2646818941241875
1105	2	-0.27530071710589255
1105	3	-0.2062588710921638
1105	4	-0.15216322419511386
1105	5	-0.10609106586062467
1105	6	-0.12197291304939739
1105	7	-0.16439381503506922
1105	8	-0.2127905912597967
1105	9	-0.22016372455792776
1105	10-11	-0.16112563673252822
1105	12-13	-0.19770291433998466
1105	14-15	-0.1954271656988027
1105	16-17	-0.23273736402078526
1105	18-19	-0.17172124856350734
1105	20-21	-0.21821728871635315
1105	22-23	-0.27895652383143954
1105	24-25	-0.24674494694487237
1105	26-27	-0.25513982239593247
1105	28-29	-0.27093054137398553
1105	30-31	-0.24385826732753912
1105	32-33	-0.25434755572575796
1105	34-35	-0.3185507689556104
1105	36-37	-0.30963291816975413
1105	38-39	-0.2830912380758974
1105	40-41	-0.3397987243739635
1105	42-43	-0.3663105491044192
1105	44-45	-0.36536901892964124
1105	46-47	-0.35669843843284355
1105	48-49	-0.48044895532246557
1105	50-51	-0.44803113040311615
1105	52-53	-0.43686501553330714
1105	54-55	-0.46329534818100626
1105	56-57	-0.5249126201526693
1105	58-59	-0.38485319720950883
1105	60-61	-0.5358464415975064
1105	62-63	-0.35612420462861394
1105	64-65	-0.3713600054328836
1105	66-67	-0.35995392512572977
1105	68-69	-0.33876088450644914
1105	70-71	-0.3790126552209401
1105	72-73	-0.32878746292303873
1105	74-75	-0.31231297208105246
1105	76-77	-0.37148400919465274
1105	78-79	-0.4156110578482597
1105	80-81	-0.36269288797637955
1105	82-83	-0.3690460618874667
1105	84-85	-0.35618232250659787
1105	86-87	-0.4541565279131454
1105	88-89	-0.4197505119321008
1105	90-91	-0.46219619642544885
1105	92-93	-0.48213655025832836
1105	94-95	-0.4866715944637612
1105	96-97	-0.43539477170276797
1105	98-99	-0.43955826738842063
1105	100	-0.3790869961928536
1106	1	-0.15846385728523416
1106	2	-0.15523490379360894
1106	3	-0.0820882269734966
1106	4	-0.039472560141803115
1106	5	-0.034694269451534865
1106	6	0.015315797007453114
1106	7	0.027420828454786772
1106	8	-0.1059922422229107
1106	9	-0.09395920046410566
1106	10-11	-0.028332255747820057
1106	12-13	-0.006379808103275764
1106	14-15	-0.0728325904193241
1106	16-17	-0.047628782647187506
1106	18-19	-0.1528117672321656
1106	20-21	-0.17235004809248977
1106	22-23	-0.08209829281924641
1106	24-25	-0.09918105964473511
1106	26-27	-0.14363375336721163
1106	28-29	-0.17114429806207454
1106	30-31	-0.07708373283157499
1106	32-33	-0.12148978851735848
1106	34-35	-0.08085801444048712
1106	36-37	-0.08765472252259343
1106	38-39	-0.16409824976352638
1106	40-41	-0.17687091833315094
1106	42-43	-0.14711824222685266
1106	44-45	-0.17920684480476723
1106	46-47	-0.18567998773819738
1106	48-49	-0.1962495575984562
1106	50-51	-0.25599280749980124
1106	52-53	-0.210284877261806
1106	54-55	-0.22637662199136344
1106	56-57	-0.21873104096764706
1106	58-59	-0.30633573042554474
1106	60-61	-0.3512908570542166
1106	62-63	-0.30408132173126035
1106	64-65	-0.21892812940228623
1106	66-67	-0.2086344535052831
1106	68-69	-0.27175433662261383
1106	70-71	-0.3005726748880022
1106	72-73	-0.2736753639990397
1106	74-75	-0.2876694933742172
1106	76-77	-0.2338682115468913
1106	78-79	-0.32181956218136776
1106	80-81	-0.3023536589832858
1106	82-83	-0.3112346193127351
1106	84-85	-0.23321912912829035
1106	86-87	-0.26917198060683134
1106	88-89	-0.25689740505988823
1106	90-91	-0.2901071505568176
1106	92-93	-0.22883459615037438
1106	94-95	-0.28184940078331167
1106	96-97	-0.2244341459690169
1106	98-99	-0.24640837625967293
1106	100	-0.1954402050796027
1107	1	-0.3903920139525461
1107	2	-0.39934561720812667
1107	3	-0.3763199378699902
1107	4	-0.2990567965553623
1107	5	-0.27366070378722895
1107	6	-0.3022553509849004
1107	7	-0.32244924654713003
1107	8	-0.21490012498612288
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2216	42-43	0.11653612490601972
2216	44-45	0.2105894244953035
2216	46-47	0.20227566001396013
2216	48-49	0.23138032945494302
2216	50-51	0.27977521478705825
2216	52-53	0.2949613955311392
2216	54-55	0.24858223114735267
2216	56-57	0.27642076868820453
2216	58-59	0.3079801435115215
2216	60-61	0.25509258716837735
2216	62-63	0.2638902128547471
2216	64-65	0.23255585402842627
2216	66-67	0.2278515085828019
2216	68-69	0.23785275499286485
2216	70-71	0.2000362276499743
2216	72-73	0.1314477272970933
2216	74-75	0.18993938843679103
2216	76-77	0.24096124200590197
2216	78-79	0.23968108150599932
2216	80-81	0.20677882354656418
2216	82-83	0.21537846623358803
2216	84-85	0.23351985161175648
2216	86-87	0.17019570114369387
2216	88-89	0.17288074541939835
2216	90-91	0.20070639044533678
2216	92-93	0.3056627960905267
2216	94-95	0.24178269637855365
2216	96-97	0.20058208055673532
2216	98-99	0.1982184366779478
2216	100	0.21629900979836592
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82682.0
3	15583.0
4	11644.0
5	9979.0
6	6936.0
7	5898.0
8	6609.0
9	7127.0
10	7205.0
11	8006.0
12	9246.0
13	10651.0
14	11750.0
15	12296.0
16	12436.0
17	12158.0
18	11709.0
19	10879.0
20	10498.0
21	10935.0
22	12881.0
23	18972.0
24	30105.0
25	40755.0
26	43340.0
27	33232.0
28	32835.0
29	41404.0
30	48142.0
31	54799.0
32	70445.0
33	94019.0
34	133338.0
35	210181.0
36	356676.0
37	677544.0
38	1098514.0
39	579263.0
40	2521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79845262751212	21.876850822954857	14.31667941497485	22.008017134558173
2	14.841067117993676	22.51890386812724	40.501945307797996	22.138083706081087
3	17.237462957719345	28.910516874535062	30.861143807903552	22.990876359842048
4	11.543018366486848	19.639242814976164	39.671928781407786	29.1458100371292
5	11.862851936474554	40.50546709175057	35.05818901464418	12.5734919571307
6	27.190005820535816	40.754321702391145	19.098474063811878	12.957198413261159
7	24.418040485224264	35.5647235809185	23.490349331549133	16.5268866023081
8	23.64664174036391	37.41848893833636	21.381745106090012	17.55312421520972
9	24.721790781058115	18.66272080864467	21.865441082334037	34.75004732796318
10-11	21.933911452613323	29.438891332457285	29.660942223524305	18.96625499140509
12-13	22.834121828127053	27.83566912281572	28.917398877506855	20.412810171550376
14-15	20.80698947630871	29.329348424562895	26.556568736429842	23.307093362698552
16-17	19.802772426056542	32.265496679480066	27.313355719016606	20.618375175446786
18-19	19.906166898723672	31.10971556589575	29.148152101615775	19.8359654337648
20-21	21.79616258030769	30.06650573420899	28.37941603827992	19.757915647203397
22-23	21.300863684352002	30.081161136671458	28.49815536968573	20.119819809290806
24-25	20.884466867651366	30.197778087127077	28.445871260365223	20.471883784856338
26-27	20.523343999335104	30.16326254986702	28.511183718417556	20.80220973238032
28-29	20.55485850184906	30.097669474302613	28.73764425051028	20.609827773338047
30-31	20.816616899929443	30.489268085467796	28.340248903380754	20.353866111222
32-33	20.424468699881178	30.290138887987066	28.73791791388815	20.54747449824361
34-35	20.78710706590509	30.041952967380936	28.640178959157087	20.530761007556883
36-37	20.940211234917218	30.160497704188515	28.221025981099977	20.678265079794286
38-39	20.702975295073028	30.23058442660645	28.5334120861403	20.533028192180225
40-41	20.57545864193755	29.975675280118676	28.719090717011085	20.72977536093269
42-43	20.516938653063452	29.97075377850273	28.708452054118496	20.803855514315327
44-45	20.79799306821451	30.02715176609867	28.557540508473807	20.61731465721301
46-47	20.78720435778479	30.268027816410235	28.401925232001467	20.5428425938035
48-49	20.738689273443764	30.002358552441038	28.44264886219326	20.81630331192194
50-51	20.662273381115572	29.988849252175214	28.408432697847203	20.94044466886201
52-53	20.68338379432642	29.983453261795734	28.482896184380092	20.850266759497753
54-55	20.816014836633393	30.273342632203704	28.334606083746976	20.576036447415927
56-57	20.80415337127015	29.990518609096444	28.182171530711535	21.023156488921863
58-59	20.732732528366714	29.80795168070365	28.485771218687912	20.97354457224173
60-61	20.896456865782767	30.12433560470475	28.17899682949987	20.800210700012613
62-63	20.749602015304376	30.045386942154277	28.329952459999276	20.87505858254207
64-65	20.79346346222074	29.889533986765482	28.40476708688355	20.912235464130234
66-67	20.65525406168718	29.91718777372649	28.2867469920198	21.14081117256653
68-69	21.082930009259034	30.11226412521638	28.066615674902835	20.738190190621758
70-71	20.97226507433413	29.869272490606296	28.172991118964735	20.985471316094838
72-73	20.778551621555117	30.00835595457529	28.392248115365415	20.82084430850418
74-75	20.79300094523911	30.15817918819556	28.23588566073954	20.812934205825783
76-77	20.905853842184964	30.041688024079082	28.11933260201917	20.933125531716783
78-79	20.987191634097744	30.0335870281142	28.275176765630487	20.70404457215757
80-81	20.894062410835772	29.965751845752266	28.250383085559864	20.889802657852094
82-83	20.783536270582424	29.937693470054878	28.52039037200148	20.758379887361222
84-85	20.754913019281933	29.963446604053797	28.24262519489408	21.03901518177019
86-87	20.98339344179263	29.955402423645804	28.14031742980817	20.920886704753393
88-89	20.824872649802316	29.854720188014518	28.291556009476054	21.02885115270712
90-91	20.782261772108498	29.841532055927416	28.208358982750603	21.16784718921348
92-93	20.920210807028774	29.943581693697137	28.117382178121137	21.018825321152956
94-95	20.74545931007329	29.832111410102613	28.37721807700129	21.04521120282281
96-97	20.781567479973784	29.949494733066683	28.212424168405775	21.056513618553755
98-99	20.746177457404468	29.900929150414058	28.15974452097358	21.193148871207896
100	20.71321161605417	30.12532531409256	28.02066424728313	21.140798822570144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18766.0
1	13518.0
2	7458.0
3	5725.0
4	3989.5
5	2796.5
6	2324.0
7	2405.5
8	2892.0
9	3004.5
10	2249.5
11	1575.0
12	1505.0
13	1540.5
14	1583.0
15	1725.5
16	1941.5
17	2393.5
18	3101.5
19	4107.5
20	5711.0
21	8126.5
22	11925.5
23	17777.0
24	24026.5
25	30150.0
26	38864.5
27	50493.5
28	65454.0
29	79321.0
30	88224.0
31	97727.5
32	108012.5
33	116970.5
34	125159.5
35	132174.5
36	135747.0
37	136809.5
38	137437.5
39	136134.5
40	135310.0
41	133519.0
42	128663.0
43	124609.0
44	121765.5
45	118234.0
46	115350.5
47	111664.5
48	107389.5
49	104718.0
50	103754.5
51	100496.5
52	93436.5
53	86743.5
54	82361.5
55	79761.5
56	73582.0
57	65302.5
58	58722.0
59	52874.0
60	46269.5
61	38879.5
62	32599.0
63	26275.5
64	20219.0
65	15927.5
66	12522.5
67	9516.0
68	7176.0
69	5509.0
70	4123.0
71	3084.5
72	2400.5
73	1871.0
74	1537.0
75	1231.0
76	925.5
77	741.5
78	566.5
79	408.5
80	306.5
81	229.0
82	165.5
83	124.0
84	114.5
85	107.0
86	98.5
87	78.0
88	67.0
89	63.0
90	52.5
91	45.0
92	37.0
93	32.5
94	27.5
95	22.5
96	19.5
97	17.5
98	15.5
99	13.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17987679309082105
2	0.1753870102016691
3	0.18815564130839021
4	0.17891655050759203
5	0.19111422656482557
6	0.07868798682028126
7	0.08037489946649441
8	0.06856651094300233
9	0.06238981540763725
10-11	0.07654690538470302
12-13	0.0758980928284672
14-15	0.07352343887264406
16-17	0.06920234724811344
18-19	0.0738608214018867
20-21	0.07587214032621777
22-23	0.07339367636139689
24-25	0.07310819883665313
26-27	0.07663773914257604
28-29	0.07422415643337876
30-31	0.07953144314338784
32-33	0.07702702667631753
34-35	0.07601487908858964
36-37	0.08709659754909758
38-39	0.08230836088407718
40-41	0.07771476798592751
42-43	0.08104966452497966
44-45	0.09175507170287084
46-47	0.08210074086608171
48-49	0.08756374258958739
50-51	0.08374872475892071
52-53	0.08277550592456698
54-55	0.0846830148399003
56-57	0.07836358054216334
58-59	0.08570813867875292
60-61	0.0822564558795783
62-63	0.07413332267550574
64-65	0.07679345415607264
66-67	0.07724762294543772
68-69	0.07521035151885722
70-71	0.07113580866569622
72-73	0.06865734470087535
74-75	0.07382189264851255
76-77	0.07906429810289804
78-79	0.07518439901660778
80-81	0.08316479345830848
82-83	0.08500742111801822
84-85	0.08682409627547855
86-87	0.08325562721618149
88-89	0.08505932612251708
90-91	0.08312586470493433
92-93	0.07388677390413613
94-95	0.07375701139288897
96-97	0.07273188755403635
98-99	0.08228240838182775
100	0.08958803776504318
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3853193.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.48965194057035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75261753088645	48.72996972563699
2	14.691395493515813	18.655031728091654
3	4.384904356904972	8.351881542377615
4	1.6614006496826483	4.219269959287551
5	0.7746346532307204	2.459064225736143
6	0.44669606088589847	1.7016346457321707
7	0.28259080073586806	1.2559114106229134
8	0.17891387758682853	0.9087343852260442
9	0.1268016090252576	0.72455310202661
>10	0.6358086394469884	7.238540760298083
>50	0.041079016599422966	1.7769550627558213
>100	0.021235199465193554	2.4611267268112607
>500	0.0013495680766570305	0.5477519223678812
>1k	5.316479604663363E-4	0.44667118356042135
>5k	0.0	0.0
>10k+	4.0895996958948184E-5	0.5229036194688826
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20139	0.5226574428013339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	7.785750674829939E-5	0.0	0.0	0.0	0.0
9	7.785750674829939E-5	0.0	0.0	0.0	0.0
10-11	7.785750674829939E-5	0.0	0.0	0.0	0.0
12-13	7.785750674829939E-5	0.0	0.0	0.0	0.0
14-15	7.785750674829939E-5	0.0	0.0	0.0	0.0
16-17	7.785750674829939E-5	0.0	0.0	0.0	0.0
18-19	7.785750674829939E-5	0.0	0.0	5.1905004498866264E-5	0.0
20-21	7.785750674829939E-5	0.0	0.0	9.083375787301596E-5	0.0
22-23	7.785750674829939E-5	0.0	0.0	1.9464376687074848E-4	0.0
24-25	1.0381000899773253E-4	0.0	0.0	3.6333503149206384E-4	0.0
26-27	1.0381000899773253E-4	0.0	0.0	6.098838028616787E-4	0.0
28-29	1.0381000899773253E-4	0.0	0.0	0.0016739363950884372	0.0
30-31	1.0381000899773253E-4	0.0	0.0	0.004866094171768713	0.0
32-33	1.0381000899773253E-4	0.0	0.0	0.011289338478503413	0.0
34-35	1.0381000899773253E-4	0.0	0.0	0.02190391189852156	0.0
36-37	1.0381000899773253E-4	0.0	0.0	0.03787767703304766	0.0
38-39	1.5571501349659879E-4	0.0	0.0	0.06136469156878464	0.0
40-41	1.5571501349659879E-4	0.0	0.0	0.08565623367425405	0.0
42-43	1.5571501349659879E-4	0.0	0.0	0.11029813456009081	0.0
44-45	1.5571501349659879E-4	0.0	0.0	0.14231054608476656	0.0
46-47	1.5571501349659879E-4	0.0	0.0	0.18244609081351493	0.0
48-49	1.5571501349659879E-4	0.0	0.0	0.22522879077170543	1.2976251124716566E-5
50-51	1.5571501349659879E-4	0.0	0.0	0.27072352721496173	2.5952502249433132E-5
52-53	1.5571501349659879E-4	0.0	0.0	0.31572516611547874	2.5952502249433132E-5
54-55	1.5571501349659879E-4	0.0	0.0	0.36565778044338815	2.5952502249433132E-5
56-57	1.5571501349659879E-4	0.0	0.0	0.4283460496268938	2.5952502249433132E-5
58-59	1.5571501349659879E-4	0.0	0.0	0.5000009083375787	2.5952502249433132E-5
60-61	1.5571501349659879E-4	0.0	0.0	0.5764699562155334	2.5952502249433132E-5
62-63	1.5571501349659879E-4	0.0	0.0	0.6575974782472614	2.5952502249433132E-5
64-65	1.5571501349659879E-4	0.0	0.0	0.7435910944507581	2.5952502249433132E-5
66-67	1.5571501349659879E-4	0.0	0.0	0.8423533417609759	2.5952502249433132E-5
68-69	1.5571501349659879E-4	0.0	0.0	0.9591396018834251	2.5952502249433132E-5
70-71	1.5571501349659879E-4	1.2976251124716566E-5	0.0	1.088577706852473	2.5952502249433132E-5
72-73	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.2266969238239558	2.5952502249433132E-5
74-75	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.3638818507144594	2.5952502249433132E-5
76-77	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.5214784206241423	2.5952502249433132E-5
78-79	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.7082456030621875	2.5952502249433132E-5
80-81	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.919148612592206	2.5952502249433132E-5
82-83	1.5571501349659879E-4	2.5952502249433132E-5	0.0	2.138304517837544	2.5952502249433132E-5
84-85	1.5571501349659879E-4	2.5952502249433132E-5	0.0	2.3665048701168097	2.5952502249433132E-5
86-87	1.5571501349659879E-4	2.5952502249433132E-5	0.0	2.605670154596461	3.8928753374149696E-5
88	1.5571501349659879E-4	2.5952502249433132E-5	0.0	2.8016764278353046	5.1905004498866264E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5045	0.0	49.14677	1
GTATCAA	9175	0.0	39.074593	1
TCAACGC	10445	0.0	33.148518	4
ATCAACG	10520	0.0	33.00334	3
CAACGCA	11240	0.0	30.887241	5
TATCAAC	11635	0.0	30.527967	2
AACGCAG	12450	0.0	28.046566	6
GTACATG	18795	0.0	26.559422	1
TACATGG	18785	0.0	25.995815	2
CATGGGT	8960	0.0	25.67412	4
CGCAGAG	13285	0.0	25.575924	8
ACATGGG	19375	0.0	24.767138	3
ACGCAGA	13880	0.0	24.71752	7
TACCTGG	2950	0.0	22.486115	2
ATGGGTG	4985	0.0	22.45954	5
GCAGAGT	14935	0.0	21.712214	9
GTACCTG	3220	0.0	21.624765	1
GTGGTAT	2720	0.0	19.026957	1
TGGTATC	2510	0.0	18.930626	2
CAGAGTA	14665	0.0	18.730953	10-11
>>END_MODULE