FastQCFastQC Report
Wed 25 May 2016
SRR2431414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853193
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280330.727526495558359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239480.6215105238694247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150710.39113016140120677No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53740.13946874708845367No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49720.12903584118418154No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47080.1221843805903312No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45150.11717554765619058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108500.065.009331
GTATCAA212400.044.433141
GTGGTAT35350.035.2509351
ATCAACG270700.034.5999763
TATCAAC273250.034.365352
TCAACGC272600.034.3579254
TGGTATC35700.033.850712
CAACGCA280850.033.298435
AACGCAG296700.031.7120236
CGCAGAG326450.028.6060968
ACGCAGA330300.028.3295767
CATGGGT88400.027.3384744
GTACATG184000.026.8595711
GCAGAGT350950.026.4216089
TACATGG183950.026.0226122
ACATGGG184300.025.2826733
ATGGGTG47050.023.583915
GAGTACT245550.023.41755112-13
CAGAGTA350800.022.74864210-11
TACCTGG28150.022.717652
Produced by FastQC (version 0.11.5)