##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431414_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3853193
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6590770304005	34.0	31.0	34.0	31.0	34.0
2	32.797072713461276	34.0	31.0	34.0	31.0	34.0
3	32.884133237032245	34.0	31.0	34.0	31.0	34.0
4	36.3379706129436	37.0	37.0	37.0	35.0	37.0
5	36.24580159882986	37.0	37.0	37.0	35.0	37.0
6	36.28542120781388	37.0	37.0	37.0	35.0	37.0
7	36.24246177131538	37.0	37.0	37.0	35.0	37.0
8	36.26068665649502	37.0	37.0	37.0	35.0	37.0
9	38.063894022438014	39.0	39.0	39.0	35.0	39.0
10-11	38.04172435691646	39.0	39.0	39.0	35.0	39.0
12-13	38.025373112636714	39.0	39.0	39.0	35.0	39.0
14-15	39.499295259801414	41.0	39.0	41.0	36.0	41.0
16-17	39.526304547942445	41.0	39.0	41.0	36.5	41.0
18-19	39.592668859307075	41.0	40.0	41.0	37.0	41.0
20-21	39.58868774546201	41.0	40.0	41.0	37.0	41.0
22-23	39.530579573875485	41.0	40.0	41.0	36.0	41.0
24-25	39.48151455169777	41.0	39.0	41.0	36.0	41.0
26-27	39.366248589157095	41.0	39.0	41.0	36.0	41.0
28-29	39.28554058932423	41.0	39.0	41.0	36.0	41.0
30-31	39.10770405219775	41.0	39.0	41.0	35.0	41.0
32-33	38.8857893959633	41.0	38.0	41.0	35.0	41.0
34-35	38.752902982020366	41.0	38.0	41.0	35.0	41.0
36-37	38.61510635465184	41.0	38.0	41.0	35.0	41.0
38-39	38.469925591580804	41.0	38.0	41.0	35.0	41.0
40-41	38.33286536646361	41.0	38.0	41.0	35.0	41.0
42-43	38.165437729176816	40.5	38.0	41.0	34.0	41.0
44-45	37.92518763010314	40.0	38.0	41.0	33.5	41.0
46-47	37.56199572666098	40.0	38.0	41.0	33.0	41.0
48-49	37.223544862663246	40.0	38.0	41.0	32.0	41.0
50-51	37.058327080943	40.0	37.0	41.0	32.0	41.0
52-53	36.999205983193676	40.0	37.0	41.0	32.0	41.0
54-55	36.862414366474766	40.0	37.0	41.0	31.0	41.0
56-57	36.71834125619973	40.0	36.0	41.0	31.0	41.0
58-59	36.5444234171504	40.0	36.0	41.0	31.0	41.0
60-61	36.391696704525316	40.0	35.5	41.0	31.0	41.0
62-63	36.23423521738984	39.0	35.0	41.0	31.0	41.0
64-65	36.00099021772333	39.0	35.0	41.0	31.0	41.0
66-67	35.73855228637652	39.0	35.0	41.0	30.0	41.0
68-69	35.446828253866336	38.0	35.0	41.0	30.0	41.0
70-71	35.14168522054307	37.0	35.0	40.0	29.5	41.0
72-73	34.81760853920372	37.0	35.0	39.5	29.0	41.0
74-75	34.47549032711312	36.0	35.0	39.0	29.0	41.0
76-77	34.13833332511504	36.0	35.0	39.0	29.0	41.0
78-79	33.81442663266543	35.5	35.0	38.5	29.0	40.0
80-81	33.540956162849874	35.0	34.5	37.0	29.0	39.0
82-83	33.330060549783	35.0	35.0	37.0	29.0	39.0
84-85	33.05983180702342	35.0	34.0	36.5	29.0	38.5
86-87	32.81470030180165	35.0	34.0	36.0	29.0	37.0
88-89	32.58188909821024	35.0	34.0	36.0	29.0	37.0
90-91	32.39911937969367	35.0	34.0	35.5	29.0	36.5
92-93	32.240625502018716	35.0	34.0	35.0	28.0	36.0
94-95	32.100508201899046	35.0	34.0	35.0	27.5	36.0
96-97	31.959729242734532	35.0	34.0	35.0	27.0	36.0
98-99	31.84557197108995	35.0	34.0	35.0	27.0	36.0
100	31.746639994415023	35.0	34.0	35.0	26.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.0024382897031856032
1101	2	0.03021065465225803
1101	3	0.02708776662875323
1101	4	0.03803757139763064
1101	5	0.037739084477493634
1101	6	0.026868592010664827
1101	7	0.053753190345616986
1101	8	0.0772496068551689
1101	9	0.05397224776222487
1101	10-11	0.08101900850240895
1101	12-13	0.04180709823222628
1101	14-15	0.03876806659057763
1101	16-17	0.0372930993023175
1101	18-19	0.03927593483242475
1101	20-21	0.020520386082345965
1101	22-23	0.04429955388194173
1101	24-25	0.050039424203383476
1101	26-27	0.05777552329463731
1101	28-29	0.03846740090961731
1101	30-31	-0.02554554846442869
1101	32-33	0.010526172248447097
1101	34-35	0.034790167739942035
1101	36-37	-0.0037701236298630647
1101	38-39	-0.0016603441428344468
1101	40-41	-0.07372567948493014
1101	42-43	-0.10655453703090245
1101	44-45	-0.1650184861456765
1101	46-47	-0.22418060868649548
1101	48-49	-0.10234167013594941
1101	50-51	-0.13223940524166267
1101	52-53	-0.061354036261242584
1101	54-55	-0.0926544881885576
1101	56-57	-0.08259805481475979
1101	58-59	-0.09758995933662362
1101	60-61	-0.10606153155342923
1101	62-63	-0.12203436069525253
1101	64-65	-0.16078183261428336
1101	66-67	-0.21135680508579213
1101	68-69	-0.12227037733542545
1101	70-71	-0.16571808240336594
1101	72-73	-0.184950174457029
1101	74-75	-0.12145191425403112
1101	76-77	-0.14427470049637492
1101	78-79	-0.0979244592152213
1101	80-81	-0.0933741829789696
1101	82-83	-0.11210100945752544
1101	84-85	-0.06393347887123468
1101	86-87	-0.11736588001953407
1101	88-89	-0.11126244067620661
1101	90-91	-0.13281784927381324
1101	92-93	-0.12324124039080431
1101	94-95	-0.1870499373073713
1101	96-97	-0.1462597668559269
1101	98-99	-0.15355382949816132
1101	100	-0.10200529120032442
1102	1	-0.06256490016677674
1102	2	-0.038456319709403886
1102	3	-0.015054406005418741
1102	4	0.007853563233432226
1102	5	0.007967732786767101
1102	6	-0.053436677403468025
1102	7	-0.034794839783856446
1102	8	-0.0024846499530823962
1102	9	-0.0202041242133717
1102	10-11	-0.021789165824529277
1102	12-13	-0.062218258157137996
1102	14-15	-0.04490618384487988
1102	16-17	-0.04708100880204569
1102	18-19	-0.0541625248173645
1102	20-21	-0.028454164076570976
1102	22-23	-0.0249562626900115
1102	24-25	-0.015310412907474813
1102	26-27	0.03077096729067108
1102	28-29	-0.06173190183154986
1102	30-31	-0.052760736402746033
1102	32-33	-0.07576572333394438
1102	34-35	-0.08279801751672977
1102	36-37	-0.016720680890578876
1102	38-39	-0.06619543645997084
1102	40-41	-0.05058738928450168
1102	42-43	-0.028304175221201433
1102	44-45	-0.12019617335785426
1102	46-47	-0.17401074390168958
1102	48-49	-0.16984109619882304
1102	50-51	-0.19638748048851085
1102	52-53	-0.19518367842889717
1102	54-55	-0.09902317968236929
1102	56-57	-0.07083513531391361
1102	58-59	-0.10977671827810553
1102	60-61	-0.18572290798426394
1102	62-63	-0.18832062591123844
1102	64-65	-0.13735446354339587
1102	66-67	-0.10401130384227741
1102	68-69	-0.08023671105430452
1102	70-71	-0.12526840947773366
1102	72-73	-0.002299801932949208
1102	74-75	-0.07637658060281893
1102	76-77	-0.06568075739079404
1102	78-79	-0.006642501020181157
1102	80-81	0.00518157253696927
1102	82-83	0.004858138050096272
1102	84-85	-0.033807794948877756
1102	86-87	-0.06215354388739058
1102	88-89	-0.09387538598594602
1102	90-91	-0.09025700495945443
1102	92-93	-0.0653235567332473
1102	94-95	-0.0757408302578284
1102	96-97	-0.11252294830075016
1102	98-99	-0.09887269261478693
1102	100	-0.048322263215720085
1103	1	-0.0936503329901015
1103	2	-0.08025140647033879
1103	3	-0.10437633468214358
1103	4	-0.01790894926140396
1103	5	-0.02810250916938628
1103	6	-0.03985919511975311
1103	7	-0.05946075576828491
1103	8	-0.05383155984291221
1103	9	-0.031063061550973714
1103	10-11	-0.039414526187712795
1103	12-13	-0.04115527967326216
1103	14-15	-0.057514233285161254
1103	16-17	0.012757332650444653
1103	18-19	-0.03955475562649724
1103	20-21	-0.029644380951786786
1103	22-23	-0.05042239060065157
1103	24-25	-0.06684487763374136
1103	26-27	-0.017840353224990224
1103	28-29	-0.008133292392372482
1103	30-31	-0.025311222296245717
1103	32-33	-0.07273282358705302
1103	34-35	-0.09340674397292759
1103	36-37	-0.031792964474945506
1103	38-39	-0.13938037515028157
1103	40-41	-0.13757866187606282
1103	42-43	-0.014944385496882262
1103	44-45	-0.02174596980340482
1103	46-47	-0.03213770244038017
1103	48-49	-0.018855402541184674
1103	50-51	-0.1198677688636991
1103	52-53	-0.06178555312420997
1103	54-55	-0.035001199875665634
1103	56-57	-0.09366336107194684
1103	58-59	-0.10012834863137954
1103	60-61	-0.05955520760858235
1103	62-63	-0.1218558042881952
1103	64-65	-0.14763833741820065
1103	66-67	-0.0659096008145994
1103	68-69	-0.05630439981796087
1103	70-71	-0.07594813333353301
1103	72-73	-0.09024493428235303
1103	74-75	-0.08489084277184844
1103	76-77	-0.01638132802038683
1103	78-79	-0.018182884841522196
1103	80-81	-0.02580106469596899
1103	82-83	-0.05212994850636932
1103	84-85	-0.04815376436982177
1103	86-87	0.020764903930277967
1103	88-89	0.012817757571319532
1103	90-91	0.016394279730832295
1103	92-93	-0.016968167149535418
1103	94-95	-0.015847751558659695
1103	96-97	-0.023839284685742257
1103	98-99	-0.02996790573262942
1103	100	0.0506392070528463
1104	1	-0.08553266220867783
1104	2	-0.07110583346013755
1104	3	-0.05485428952690796
1104	4	-0.020485399981964747
1104	5	-0.023110291295076024
1104	6	-0.06561188735716428
1104	7	-0.09691814311650404
1104	8	-0.11152641229758586
1104	9	-0.05589497393747678
1104	10-11	-0.07198169821995748
1104	12-13	-0.0577281354928445
1104	14-15	-0.12585476587241828
1104	16-17	-0.06759823487760741
1104	18-19	-0.06717933265063891
1104	20-21	-0.03362838821090719
1104	22-23	-0.056291672943729054
1104	24-25	0.013796218291197704
1104	26-27	-0.035893624553366976
1104	28-29	0.001716452719385586
1104	30-31	-0.0072382593542883455
1104	32-33	0.02583906182981366
1104	34-35	-0.012721538833581292
1104	36-37	0.057615231647218934
1104	38-39	-0.0407186959755137
1104	40-41	-0.07134946409664167
1104	42-43	-0.04957405713528118
1104	44-45	-0.06052742124860799
1104	46-47	-0.013208941972322918
1104	48-49	0.006952032381967399
1104	50-51	-0.13550172854787945
1104	52-53	-0.0700264934066368
1104	54-55	0.01156080192650677
1104	56-57	-0.08185036208645613
1104	58-59	-0.039742834097395985
1104	60-61	-0.057530909732754765
1104	62-63	-0.08283765964778667
1104	64-65	-0.023722557661805865
1104	66-67	-0.044724181430495946
1104	68-69	-0.09400478167772519
1104	70-71	-0.08646542414558667
1104	72-73	-0.07113343564029151
1104	74-75	-0.05596661925155644
1104	76-77	-0.11837443438030704
1104	78-79	-0.13753139174461637
1104	80-81	-0.09515847220840357
1104	82-83	-0.08775247130137842
1104	84-85	-0.06138810402337924
1104	86-87	-0.08859168441102838
1104	88-89	-0.06832153617714454
1104	90-91	-0.07575932115639716
1104	92-93	-0.13979815133323115
1104	94-95	-0.11317040101013731
1104	96-97	-0.08872945274185184
1104	98-99	-0.12339673551517194
1104	100	-0.0843349890567211
1105	1	-0.1487895677515283
1105	2	-0.17495414813235755
1105	3	-0.09657078520140061
1105	4	-0.04642824640138343
1105	5	-0.11213229065632646
1105	6	-0.021621032582785915
1105	7	-0.02706749026908284
1105	8	-0.06018709015858548
1105	9	-0.03512094003743016
1105	10-11	-0.07802193558087112
1105	12-13	-0.09501116076052085
1105	14-15	-0.14258084853145903
1105	16-17	-0.10268179025599267
1105	18-19	-0.15446081351047525
1105	20-21	-0.12268404400727206
1105	22-23	-0.1607255513073369
1105	24-25	-0.162812209629827
1105	26-27	-0.10520329755851776
1105	28-29	-0.14902838895841342
1105	30-31	-0.1362325934172759
1105	32-33	-0.11751200013999608
1105	34-35	-0.19132487545387988
1105	36-37	-0.25158658341081264
1105	38-39	-0.11824403592007116
1105	40-41	-0.16491479080009697
1105	42-43	-0.21238057838284163
1105	44-45	-0.3361768301775072
1105	46-47	-0.2731960155700648
1105	48-49	-0.2439001602109343
1105	50-51	-0.2549575957824288
1105	52-53	-0.24956259408570958
1105	54-55	-0.22361754512583332
1105	56-57	-0.20179271779213792
1105	58-59	-0.18353536359267508
1105	60-61	-0.22983918169773432
1105	62-63	-0.24169049681241717
1105	64-65	-0.25476422287148637
1105	66-67	-0.31148042375216534
1105	68-69	-0.2838978853430447
1105	70-71	-0.2523773707522068
1105	72-73	-0.1722729172321138
1105	74-75	-0.1792814663143787
1105	76-77	-0.22981074533196022
1105	78-79	-0.11733141132654623
1105	80-81	-0.13485281349804978
1105	82-83	-0.17711628017246284
1105	84-85	-0.1841401164159322
1105	86-87	-0.24743563394560653
1105	88-89	-0.1736434490515606
1105	90-91	-0.1935488306058346
1105	92-93	-0.2587810800899888
1105	94-95	-0.2693639427044836
1105	96-97	-0.28715351566510705
1105	98-99	-0.32836850490535596
1105	100	-0.287152746599844
1106	1	-0.19591443019913868
1106	2	-0.14035421792591052
1106	3	-0.1564917824687484
1106	4	-0.033009711419317966
1106	5	-0.1217049333679796
1106	6	-0.17263710851281644
1106	7	-0.11745738648183135
1106	8	-0.11139309151209176
1106	9	-0.09997709954134137
1106	10-11	-0.12478849287457194
1106	12-13	-0.12623541323925025
1106	14-15	-0.1489780797566027
1106	16-17	-0.22049756984215918
1106	18-19	-0.2097698339367895
1106	20-21	-0.18885691572204166
1106	22-23	-0.1869813335246988
1106	24-25	-0.13448394357591553
1106	26-27	-0.14746351601456809
1106	28-29	-0.17863127925878075
1106	30-31	-0.2192849686836098
1106	32-33	-0.18494110003730668
1106	34-35	-0.22013339026717205
1106	36-37	-0.204564014227671
1106	38-39	-0.22806184799257778
1106	40-41	-0.29875159095529114
1106	42-43	-0.2551390235698179
1106	44-45	-0.2605791810829743
1106	46-47	-0.3141845364261826
1106	48-49	-0.29773693806787094
1106	50-51	-0.2812448742910263
1106	52-53	-0.34170680584781365
1106	54-55	-0.25603754175878635
1106	56-57	-0.2773339244178459
1106	58-59	-0.25836539511126233
1106	60-61	-0.30534680157038707
1106	62-63	-0.31783515008005736
1106	64-65	-0.24187457472687157
1106	66-67	-0.2662719573260972
1106	68-69	-0.3030596157325647
1106	70-71	-0.31586344158708357
1106	72-73	-0.29278316272480254
1106	74-75	-0.2687073809053544
1106	76-77	-0.28744087518587946
1106	78-79	-0.32657242317695534
1106	80-81	-0.27702762962103833
1106	82-83	-0.28715331200657346
1106	84-85	-0.3362524305181296
1106	86-87	-0.3415678748872182
1106	88-89	-0.3621134930125791
1106	90-91	-0.34202053578717084
1106	92-93	-0.3680825480958845
1106	94-95	-0.3991042739005266
1106	96-97	-0.32261332249528607
1106	98-99	-0.3442465378867148
1106	100	-0.26532971896449453
1107	1	-0.2222575184155744
1107	2	-0.1891836894349197
1107	3	-0.13150012619356488
1107	4	-0.19212660942900328
1107	5	-0.20655513611420417
1107	6	-0.1621022802426637
1107	7	-0.16395357890519335
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2216	28-29	0.023259950625508452
2216	30-31	-0.08710072478405095
2216	32-33	-0.06732642427226665
2216	34-35	0.01707668173216348
2216	36-37	0.01937398796938794
2216	38-39	0.08359501558792459
2216	40-41	0.0904068444199595
2216	42-43	0.048616452762679785
2216	44-45	0.03345597299896497
2216	46-47	0.035008714490608384
2216	48-49	0.047472945469621664
2216	50-51	0.1187067915811042
2216	52-53	0.0319804864507347
2216	54-55	0.036968221104785925
2216	56-57	-0.038714610802763616
2216	58-59	0.019397653088141453
2216	60-61	-0.02173044922428602
2216	62-63	-0.029899049006168354
2216	64-65	-0.04493891237371628
2216	66-67	-0.028348964137585142
2216	68-69	-0.02335840451021909
2216	70-71	-0.052826757164318394
2216	72-73	-0.0038461700076339866
2216	74-75	0.0019985199844470003
2216	76-77	-0.0357860766250937
2216	78-79	0.027153555615527125
2216	80-81	-0.019004231565837415
2216	82-83	-0.049630608304660484
2216	84-85	-0.00485773999857031
2216	86-87	0.024128357257843902
2216	88-89	0.052762978122053994
2216	90-91	0.027570878183254877
2216	92-93	0.016459723778929458
2216	94-95	-0.01944051515010159
2216	96-97	-0.013578611740065583
2216	98-99	0.015997063430905456
2216	100	0.027257858214657205
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56.0
3	347.0
4	78.0
5	133.0
6	300.0
7	724.0
8	1328.0
9	2387.0
10	3453.0
11	4759.0
12	7210.0
13	10758.0
14	12439.0
15	16208.0
16	21187.0
17	31896.0
18	38295.0
19	19824.0
20	11663.0
21	10275.0
22	10069.0
23	10001.0
24	10903.0
25	12732.0
26	15540.0
27	19996.0
28	24589.0
29	31163.0
30	39763.0
31	50303.0
32	64049.0
33	83257.0
34	115766.0
35	189108.0
36	306127.0
37	643008.0
38	1224691.0
39	800642.0
40	8166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5151184039878	21.07806661539863	14.91056340422528	22.496251576388293
2	14.392194989270731	22.00481078179876	40.772777245438114	22.830216983492395
3	16.297354975488943	28.43988529930896	32.267238388034336	22.995521337167762
4	10.952682619323765	18.853558594132192	40.04442549335058	30.149333293193465
5	11.135229562944227	39.054677280401066	35.884500080750655	13.925593075904057
6	26.888998293103928	40.443055927901874	18.647885013805436	14.020060765188767
7	23.738494282533992	35.40154879342924	23.80716460348599	17.052792320550775
8	24.482189186993747	35.27422581739352	21.398772394738597	18.844812600874132
9	25.518660892060403	15.91581732755596	22.514632024565607	36.050889755818034
10-11	22.438442611102012	27.6502370890843	29.927841662745674	19.98347863706801
12-13	24.21241292611089	26.131613443707597	29.839499345088605	19.81647428509291
14-15	21.537592329270815	27.170401274994532	28.729575705135975	22.56243069059868
16-17	19.035835993681086	30.402499952636685	29.38886009602945	21.172803957652782
18-19	18.94205594039537	27.952069849647977	33.4890527732797	19.616821436676958
20-21	20.643087434239604	27.005057883163392	33.30174481267873	19.050109869918273
22-23	20.135869205549376	27.05911801413426	33.479368139290266	19.3256446410261
24-25	19.76074641492041	27.23007314455132	33.38692958980503	19.622250850723244
26-27	19.51638234153351	27.22628285639283	33.39985832512477	19.857476476948897
28-29	19.532319427652784	27.171907503544794	33.610294651010186	19.68547841779224
30-31	19.808164094727772	27.510693090839823	33.21409876338794	19.46704405104447
32-33	19.47205533135227	27.341279213111353	33.53121472042355	19.65545073511283
34-35	19.808889773586365	27.22019200299805	33.22293935199831	19.747978871417267
36-37	19.98825377554434	27.357270785769472	32.756407544678346	19.89806789400784
38-39	19.837556854257546	27.496185926187668	32.87110707863811	19.79515014091668
40-41	19.77533671140148	27.3556432549303	32.87033582706893	19.998684206599286
42-43	19.785130528020193	27.383355005928227	32.67275713946662	20.15875732658496
44-45	20.143782202222628	27.586474476076827	32.20270274829498	20.067040573405563
46-47	20.253190345241396	27.93837207008846	31.592566986090482	20.21587059857967
48-49	20.36451351159513	27.959738819038403	31.090754531861325	20.58499313750514
50-51	20.386309088795137	28.061831461980535	30.79127547503571	20.760583974188616
52-53	20.378708860831768	28.213346877156503	30.74650722742385	20.661437034587877
54-55	20.499503460335806	28.478032288508686	30.611550266514563	20.410913984640942
56-57	20.390588027059156	28.18690651079964	30.552642687530486	20.869862774610716
58-59	20.27992236533218	27.91147197941023	30.957738623636534	20.85086703162106
60-61	20.498310283646298	28.104298249632155	30.684296518586102	20.713094948135446
62-63	20.334697324502816	28.005596974737312	30.854523555124857	20.805182145635015
64-65	20.301549297072295	27.895120193702038	31.011972200814686	20.791358308410985
66-67	20.267205234740494	27.948002954961492	30.796909202670363	20.987882607627647
68-69	20.718566774906154	28.11552731455337	30.44580793442514	20.720097976115337
70-71	20.597461813069636	27.894986055037347	30.502077048757215	21.005475083135803
72-73	20.449812064662318	28.011734182638943	30.663850469754	20.874603282944744
74-75	20.49759263277113	28.17922480491398	30.399097159431243	20.924085402883648
76-77	20.58797385888923	28.108091414212915	30.325684252515046	20.978250474382808
78-79	20.62246726166648	28.113410100497866	30.441144964797136	20.822977673038526
80-81	20.533911385892257	28.080391002986506	30.419310865670905	20.966386745450333
82-83	20.3779200022008	28.07328613637151	30.626928376791575	20.921865484636115
84-85	20.361189351386745	28.10744378833056	30.324442879206387	21.206923981076304
86-87	20.458476734103634	28.08052883773162	30.31529699628536	21.14569743187939
88-89	20.304179499773237	27.976624335527855	30.40855507276185	21.310641091937057
90-91	20.233447534061032	27.92582809369045	30.349334325074217	21.491390047174306
92-93	20.279261841845052	28.047240730849865	30.285158303915903	21.388339123389176
94-95	20.10546644923522	27.862796553018637	30.5274347592118	21.50430223853434
96-97	20.098798709017053	27.956292296145424	30.359743671993165	21.585165322844357
98-99	20.073458805454198	27.87668886478182	30.308355695814885	21.7414966339491
100	20.065836427309762	28.093816389865427	30.06958397544266	21.770763207382153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4172.0
1	2353.5
2	2200.0
3	3811.0
4	2344.0
5	1304.5
6	3198.5
7	8421.5
8	14707.0
9	16274.0
10	10669.5
11	5119.5
12	4170.5
13	4526.5
14	5042.0
15	5479.0
16	5983.0
17	6593.5
18	7450.5
19	8333.5
20	9658.5
21	11995.5
22	15541.5
23	20924.5
24	26798.0
25	32410.5
26	40296.0
27	51322.5
28	65542.5
29	78443.0
30	87053.0
31	96478.5
32	106358.0
33	115261.0
34	123590.5
35	130119.5
36	133100.5
37	134335.5
38	134940.0
39	134012.0
40	134247.0
41	132117.0
42	127069.5
43	123520.0
44	120870.0
45	117801.0
46	114761.5
47	110738.5
48	106769.0
49	104226.0
50	103082.0
51	99712.0
52	91994.5
53	85145.0
54	80626.5
55	77036.0
56	70572.0
57	62860.0
58	56816.0
59	51219.0
60	44455.0
61	36996.0
62	31276.0
63	25052.0
64	18604.0
65	14385.5
66	11252.5
67	8428.0
68	6329.5
69	4679.5
70	3370.5
71	2453.5
72	1836.0
73	1312.0
74	1052.0
75	823.0
76	519.0
77	377.0
78	270.0
79	147.0
80	72.5
81	34.0
82	9.0
83	5.5
84	4.5
85	2.0
86	1.5
87	2.5
88	1.5
89	1.0
90	1.5
91	1.0
92	1.0
93	2.0
94	2.5
95	1.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006228600539863952
2	0.004697402907147397
3	8.823850764807264E-4
4	0.0
5	0.047519031618712064
6	0.0
7	0.0
8	0.0
9	2.5952502249433132E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	6.488125562358284E-5
20-21	0.0
22-23	5.1905004498866264E-5
24-25	2.5952502249433132E-5
26-27	1.1678626012244908E-4
28-29	1.946437668707485E-4
30-31	1.0381000899773253E-4
32-33	0.0011159575967256247
34-35	6.228600539863951E-4
36-37	0.0010381000899773254
38-39	7.655988163582773E-4
40-41	1.1678626012244908E-4
42-43	4.282162871156467E-4
44-45	1.0381000899773253E-4
46-47	1.2976251124716568E-4
48-49	6.488125562358284E-5
50-51	2.3357252024489817E-4
52-53	5.709550494875289E-4
54-55	6.87741309609978E-4
56-57	0.0020632239288299343
58-59	4.5416878936507983E-4
60-61	8.175038208571438E-4
62-63	0.0010381000899773254
64-65	0.0015182213815918381
66-67	3.244062781179141E-4
68-69	2.3357252024489817E-4
70-71	2.4654877136961475E-4
72-73	1.5571501349659879E-4
74-75	5.450025472380958E-4
76-77	7.396463141088442E-4
78-79	6.87741309609978E-4
80-81	6.617888073605449E-4
82-83	0.0012846488613469402
84-85	8.823850764807264E-4
86-87	0.0016609601439637205
88-89	0.0010510763411020418
90-91	9.342900809795927E-4
92-93	9.083375787301597E-4
94-95	6.358363051111117E-4
96-97	1.2976251124716568E-4
98-99	3.3738252924263073E-4
100	1.8166751574603192E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3853193.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.10586979457435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.18508669765006	43.847740750505146
2	16.264260133296812	19.226264834874556
3	4.797386194916848	8.506610513731312
4	1.8820587168915257	4.449628698653368
5	0.8943332401425164	2.6430172022411687
6	0.4833870527247563	1.7142607319239496
7	0.31167135727533707	1.2895124663270034
8	0.21290609875925262	1.006720012138814
9	0.1554797194508008	0.8270787648200384
>10	0.7331539507946978	7.809540222646332
>50	0.050038498704063246	2.03274170655585
>100	0.02728966386307435	2.9853067389832306
>500	0.0017163932608707563	0.6661031742294922
>1k	0.0010562419452934389	1.1114898731142913
>5k	4.401008105389282E-5	0.13979139643137362
>10k+	1.3203024316167848E-4	1.7441929128240539
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28033	0.727526495558359	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23948	0.6215105238694247	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15071	0.39113016140120677	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5374	0.13946874708845367	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4972	0.12903584118418154	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4708	0.1221843805903312	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4515	0.11717554765619058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.5952502249433132E-5	0.0
12-13	0.0	0.0	0.0	5.1905004498866264E-5	0.0
14-15	2.5952502249433132E-5	0.0	0.0	5.1905004498866264E-5	0.0
16-17	2.5952502249433132E-5	0.0	0.0	5.1905004498866264E-5	0.0
18-19	2.5952502249433132E-5	0.0	0.0	9.083375787301596E-5	2.5952502249433132E-5
20-21	2.5952502249433132E-5	0.0	0.0	1.167862601224491E-4	2.5952502249433132E-5
22-23	2.5952502249433132E-5	0.0	0.0	2.4654877136961475E-4	2.5952502249433132E-5
24-25	2.5952502249433132E-5	0.0	0.0	3.8928753374149695E-4	2.5952502249433132E-5
26-27	2.5952502249433132E-5	0.0	0.0	6.358363051111118E-4	2.5952502249433132E-5
28-29	2.5952502249433132E-5	0.0	0.0	0.0017128651484625869	2.5952502249433132E-5
30-31	2.5952502249433132E-5	0.0	0.0	0.004879070422893429	2.5952502249433132E-5
32-33	2.5952502249433132E-5	0.0	0.0	0.01130231472962813	2.5952502249433132E-5
34-35	2.5952502249433132E-5	0.0	0.0	0.02195581690302043	2.5952502249433132E-5
36-37	2.5952502249433132E-5	0.0	0.0	0.03804636829766897	2.5952502249433132E-5
38-39	2.5952502249433132E-5	0.0	0.0	0.061702074098027276	2.5952502249433132E-5
40-41	2.5952502249433132E-5	0.0	0.0	0.08614933121699328	2.5952502249433132E-5
42-43	2.5952502249433132E-5	0.0	0.0	0.1109080183629525	2.5952502249433132E-5
44-45	2.5952502249433132E-5	0.0	0.0	0.14349138493711577	2.5952502249433132E-5
46-47	2.5952502249433132E-5	0.0	0.0	0.18386050218610905	2.5952502249433132E-5
48-49	2.5952502249433132E-5	0.0	0.0	0.22690272716679388	2.5952502249433132E-5
50-51	2.5952502249433132E-5	0.0	0.0	0.27238448735892545	2.5952502249433132E-5
52-53	3.8928753374149696E-5	0.0	0.0	0.3175677937751885	2.5952502249433132E-5
54-55	6.488125562358283E-5	0.0	0.0	0.3680713631525854	2.5952502249433132E-5
56-57	7.785750674829939E-5	1.2976251124716566E-5	0.0	0.43155118365469886	2.5952502249433132E-5
58-59	1.5571501349659879E-4	2.5952502249433132E-5	0.0	0.5037510449126218	2.5952502249433132E-5
60-61	1.5571501349659879E-4	2.5952502249433132E-5	0.0	0.5813490266384269	2.5952502249433132E-5
62-63	1.5571501349659879E-4	2.5952502249433132E-5	0.0	0.6633200049932615	3.8928753374149696E-5
64-65	1.5571501349659879E-4	2.5952502249433132E-5	0.0	0.7503127925333613	5.1905004498866264E-5
66-67	1.5571501349659879E-4	2.5952502249433132E-5	0.0	0.8498017099065631	5.1905004498866264E-5
68-69	1.5571501349659879E-4	2.5952502249433132E-5	0.0	0.9683008351774749	5.1905004498866264E-5
70-71	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.099373947788237	5.1905004498866264E-5
72-73	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.23977698495767	5.1905004498866264E-5
74-75	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.3791289457860012	5.1905004498866264E-5
76-77	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.5389185021357612	5.1905004498866264E-5
78-79	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.728151172287503	5.1905004498866264E-5
80-81	1.5571501349659879E-4	2.5952502249433132E-5	0.0	1.9420906245807048	5.1905004498866264E-5
82-83	1.5571501349659879E-4	2.5952502249433132E-5	0.0	2.163893685055485	5.1905004498866264E-5
84-85	1.5571501349659879E-4	2.5952502249433132E-5	0.0	2.3949488125821885	5.1905004498866264E-5
86-87	1.5571501349659879E-4	2.5952502249433132E-5	0.0	2.638889357475735	5.1905004498866264E-5
88	1.5571501349659879E-4	2.5952502249433132E-5	0.0	2.8383992185182523	5.1905004498866264E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10850	0.0	65.00933	1
GTATCAA	21240	0.0	44.43314	1
GTGGTAT	3535	0.0	35.250935	1
ATCAACG	27070	0.0	34.599976	3
TATCAAC	27325	0.0	34.36535	2
TCAACGC	27260	0.0	34.357925	4
TGGTATC	3570	0.0	33.85071	2
CAACGCA	28085	0.0	33.29843	5
AACGCAG	29670	0.0	31.712023	6
CGCAGAG	32645	0.0	28.606096	8
ACGCAGA	33030	0.0	28.329576	7
CATGGGT	8840	0.0	27.338474	4
GTACATG	18400	0.0	26.859571	1
GCAGAGT	35095	0.0	26.421608	9
TACATGG	18395	0.0	26.022612	2
ACATGGG	18430	0.0	25.282673	3
ATGGGTG	4705	0.0	23.58391	5
GAGTACT	24555	0.0	23.417551	12-13
CAGAGTA	35080	0.0	22.748642	10-11
TACCTGG	2815	0.0	22.71765	2
>>END_MODULE