FastQCFastQC Report
Wed 25 May 2016
SRR2431413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541583
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA337281.3270469624639447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28060.11040363427045272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52800.050.3462031
GTATCAA89950.039.857131
ATCAACG109350.031.881193
TCAACGC109750.031.6345184
TATCAAC112500.031.6158122
CAACGCA116500.029.7612365
GTACATG196600.028.5023371
AACGCAG126400.027.5886066
TACATGG197150.027.3716982
CATGGGT99000.025.9933074
TACCTGG19750.025.2507252
CGCAGAG135600.025.1944488
ACATGGG203500.025.1304453
ACGCAGA142050.024.416287
ATGGGTG46200.023.8277635
TGGTATC24550.023.571582
GTGGTAT25350.023.5711
GTACCTG23000.022.706351
GCAGAGT144700.022.6366169
ATGGGTA41800.022.6219065
Produced by FastQC (version 0.11.5)