##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431413_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2541583
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.19780860983096	31.0	31.0	34.0	25.0	34.0
2	30.294999612446258	33.0	31.0	34.0	25.0	34.0
3	30.389233402961853	33.0	31.0	34.0	25.0	34.0
4	33.397479051441564	37.0	35.0	37.0	25.0	37.0
5	33.67391070840496	37.0	35.0	37.0	28.0	37.0
6	33.72381110512622	37.0	35.0	37.0	28.0	37.0
7	33.68232947733755	37.0	35.0	37.0	27.0	37.0
8	33.60597430813788	37.0	35.0	37.0	27.0	37.0
9	34.896918573975356	39.0	37.0	39.0	23.0	39.0
10-11	34.9686707064062	39.0	37.0	39.0	26.0	39.0
12-13	34.899019823472216	39.0	37.0	39.0	25.5	39.0
14-15	36.22437728769826	40.5	38.0	41.0	25.5	41.0
16-17	36.2804525762094	40.0	38.0	41.0	26.5	41.0
18-19	36.32697751755501	40.0	38.0	41.0	26.5	41.0
20-21	36.456574504944356	40.5	38.0	41.0	27.0	41.0
22-23	36.49685825723574	40.0	37.5	41.0	27.0	41.0
24-25	36.61593857056803	40.0	37.0	41.0	28.0	41.0
26-27	36.71536951577029	40.0	37.0	41.0	29.0	41.0
28-29	36.71438036845541	40.0	37.0	41.0	29.5	41.0
30-31	36.65795470775497	40.0	37.0	41.0	29.5	41.0
32-33	36.5958097768202	40.0	37.0	41.0	29.5	41.0
34-35	36.519280503528705	40.0	37.0	41.0	29.0	41.0
36-37	36.38400555874036	40.0	36.5	41.0	28.0	41.0
38-39	36.24878235336009	40.0	36.0	41.0	27.5	41.0
40-41	36.18364696332955	40.0	36.0	41.0	27.5	41.0
42-43	36.018140072545336	40.0	36.0	41.0	27.0	41.0
44-45	35.878088970535295	40.0	35.0	41.0	27.0	41.0
46-47	35.75513784125877	40.0	35.0	41.0	27.0	41.0
48-49	35.62442816150407	40.0	35.0	41.0	27.0	41.0
50-51	35.49988334042209	40.0	35.0	41.0	27.0	41.0
52-53	35.34144645286028	40.0	35.0	41.0	26.5	41.0
54-55	35.181891364555085	39.0	35.0	41.0	26.0	41.0
56-57	34.99662749554116	39.0	34.0	41.0	26.0	41.0
58-59	34.77142218058588	39.0	34.0	41.0	26.0	41.0
60-61	34.522444869988504	38.5	34.0	41.0	25.5	41.0
62-63	34.381420555614355	38.0	34.0	41.0	25.0	41.0
64-65	34.09378151333244	38.0	34.0	40.0	24.5	41.0
66-67	33.78913712438272	37.0	33.0	40.0	23.0	41.0
68-69	33.44934731621986	37.0	33.0	40.0	22.0	41.0
70-71	33.07125244385094	36.0	33.0	39.5	20.5	41.0
72-73	32.69815701474239	36.0	32.5	39.0	19.0	41.0
74-75	32.39366115527213	35.0	32.0	39.0	18.0	41.0
76-77	32.11179489318272	35.0	32.0	38.5	15.5	40.0
78-79	31.76154919984907	35.0	32.0	37.0	11.5	39.0
80-81	31.441884644333868	35.0	32.0	37.0	7.5	39.0
82-83	31.1329024076727	35.0	32.0	36.0	4.5	39.0
84-85	30.848551670356624	35.0	31.0	36.0	2.0	37.0
86-87	30.58440015533626	35.0	31.0	36.0	2.0	37.0
88-89	30.336194804576518	35.0	31.0	35.0	2.0	37.0
90-91	30.12168656305932	35.0	31.0	35.0	2.0	36.0
92-93	29.900532463429286	35.0	31.0	35.0	2.0	36.0
94-95	29.700388498034492	35.0	30.5	35.0	2.0	36.0
96-97	29.514298962496994	35.0	30.0	35.0	2.0	36.0
98-99	29.31225854123198	35.0	30.0	35.0	2.0	35.0
100	29.144989559656324	35.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4020734835725577
1101	2	0.35983269355320857
1101	3	0.3145419581120592
1101	4	0.5344246389710321
1101	5	0.40274892656326955
1101	6	0.41817669033692084
1101	7	0.4136652711970754
1101	8	0.3588878494218761
1101	9	0.464080370878996
1101	10-11	0.3778199597846452
1101	12-13	0.2831021267406584
1101	14-15	0.37809068618418706
1101	16-17	0.3388509916452378
1101	18-19	0.34853545468661906
1101	20-21	0.3580858607955548
1101	22-23	0.35370106241332877
1101	24-25	0.37780956737606175
1101	26-27	0.34044697873350316
1101	28-29	0.36724260947060827
1101	30-31	0.4191706251141909
1101	32-33	0.45137052151497414
1101	34-35	0.4154289691406561
1101	36-37	0.4072039251102879
1101	38-39	0.4881564355812884
1101	40-41	0.42065101428968177
1101	42-43	0.46207945639568493
1101	44-45	0.3229201368819261
1101	46-47	0.3609811190672332
1101	48-49	0.41479859549927767
1101	50-51	0.3447955611402378
1101	52-53	0.24636920471300527
1101	54-55	0.36755833684402717
1101	56-57	0.3123257878903871
1101	58-59	0.3662490121425961
1101	60-61	0.3685293686843565
1101	62-63	0.3097893983037565
1101	64-65	0.4326255574704305
1101	66-67	0.40377184278884926
1101	68-69	0.48895608530958157
1101	70-71	0.39548909036910374
1101	72-73	0.35133747745307886
1101	74-75	0.44964896592982484
1101	76-77	0.45730089614296077
1101	78-79	0.42929431162929177
1101	80-81	0.42854369532573244
1101	82-83	0.33805694900490835
1101	84-85	0.21616535754397503
1101	86-87	0.2255732540387072
1101	88-89	0.29153386395600833
1101	90-91	0.26447393161458876
1101	92-93	0.21021771042793702
1101	94-95	0.20405626460974347
1101	96-97	0.199274602064758
1101	98-99	0.20344945266749903
1101	100	0.2277101094117171
1102	1	0.08532036544250943
1102	2	0.17804358593739522
1102	3	0.09870242645056493
1102	4	0.2171128584793891
1102	5	0.15332540859728283
1102	6	0.10451247173893563
1102	7	0.11477340472081465
1102	8	0.12587296718177043
1102	9	0.21904143020537248
1102	10-11	0.1748067625977754
1102	12-13	0.16376565631131257
1102	14-15	0.11540729987119391
1102	16-17	0.05890854918714439
1102	18-19	0.02944694343584331
1102	20-21	0.09199602526174999
1102	22-23	0.0523840010461214
1102	24-25	-4.5018217312531306E-4
1102	26-27	0.04494491415250934
1102	28-29	0.015295716471172227
1102	30-31	0.06698777776813358
1102	32-33	0.08759292915904382
1102	34-35	0.06242493189066067
1102	36-37	0.014064045683817028
1102	38-39	0.013278656650264509
1102	40-41	0.045869227120341804
1102	42-43	0.07718328568697785
1102	44-45	0.03398899834866853
1102	46-47	-0.08357109444871469
1102	48-49	-0.20585418599527827
1102	50-51	-0.14198544634026433
1102	52-53	-0.18070925686778594
1102	54-55	-0.22847070547050663
1102	56-57	-0.30895927934917466
1102	58-59	-0.2682723983501276
1102	60-61	-0.22959489195554994
1102	62-63	-0.25350701600300596
1102	64-65	-0.25159163682526753
1102	66-67	-0.2328533832396289
1102	68-69	-0.25192071135106886
1102	70-71	-0.2890763691033271
1102	72-73	-0.2876588591974283
1102	74-75	-0.23357220246545296
1102	76-77	-0.2377346369090958
1102	78-79	-0.28150686396889313
1102	80-81	-0.3086737378161075
1102	82-83	-0.34640143612010377
1102	84-85	-0.3514802291796819
1102	86-87	-0.38164826199649937
1102	88-89	-0.4875220497992778
1102	90-91	-0.43419915930450514
1102	92-93	-0.5396112832420172
1102	94-95	-0.5437648114983915
1102	96-97	-0.526818119005469
1102	98-99	-0.5571107353081608
1102	100	-0.6386057702298373
1103	1	-0.3059861655849083
1103	2	-0.30241839789105285
1103	3	-0.1929536958655369
1103	4	-0.08755166532954917
1103	5	-0.10994219964917562
1103	6	-0.06529113921997975
1103	7	-0.08757186358888447
1103	8	-0.17099846258302165
1103	9	-0.0862655823815075
1103	10-11	-0.06838875300253733
1103	12-13	-0.06346537982776113
1103	14-15	-0.05515527399045084
1103	16-17	-0.11914445490857162
1103	18-19	-0.18713649964063706
1103	20-21	-0.30071372336911395
1103	22-23	-0.24739828370785233
1103	24-25	-0.19633271053231738
1103	26-27	-0.14941024455328034
1103	28-29	-0.2160787887633262
1103	30-31	-0.13164882234832476
1103	32-33	-0.17020945517454322
1103	34-35	-0.21948045580156617
1103	36-37	-0.3777198219512101
1103	38-39	-0.22073290876196694
1103	40-41	-0.1729600858466256
1103	42-43	-0.29908994464340566
1103	44-45	-0.38435549971158167
1103	46-47	-0.5014073906421856
1103	48-49	-0.43290233395687494
1103	50-51	-0.5057488028588821
1103	52-53	-0.513637758800165
1103	54-55	-0.4519786389170193
1103	56-57	-0.4706589170739548
1103	58-59	-0.5251362331113469
1103	60-61	-0.44739559328795053
1103	62-63	-0.4594497761495333
1103	64-65	-0.46925866548051687
1103	66-67	-0.43012799621077846
1103	68-69	-0.5146719791300711
1103	70-71	-0.5708366440099084
1103	72-73	-0.5563842437693438
1103	74-75	-0.5430393452453757
1103	76-77	-0.5587972893926896
1103	78-79	-0.6496446415719532
1103	80-81	-0.5500317723655428
1103	82-83	-0.6055299516884816
1103	84-85	-0.582658870543181
1103	86-87	-0.6261730567809138
1103	88-89	-0.7117157755216788
1103	90-91	-0.6913855437019798
1103	92-93	-0.7003573226610982
1103	94-95	-0.7967839252324183
1103	96-97	-0.7430158999008256
1103	98-99	-0.8053703800106398
1103	100	-0.7837154847093011
1104	1	-0.022670881092668793
1104	2	0.07023573808798744
1104	3	0.09715917656997419
1104	4	0.11710081299651165
1104	5	0.1483682111961926
1104	6	0.15575288483069727
1104	7	0.18640770510256033
1104	8	0.11690831834562943
1104	9	0.16396377021427355
1104	10-11	0.11201556964637405
1104	12-13	0.10531793875180995
1104	14-15	0.13031622375787322
1104	16-17	0.11583494243894421
1104	18-19	0.0842672010818859
1104	20-21	0.07366779858190853
1104	22-23	0.10124915667733347
1104	24-25	0.14209681500130955
1104	26-27	0.12986374451749327
1104	28-29	0.22107043893223022
1104	30-31	0.1647918598518956
1104	32-33	0.14524810516978448
1104	34-35	0.221180709570163
1104	36-37	0.03860927825709837
1104	38-39	0.08233194844324743
1104	40-41	0.1783096217713407
1104	42-43	0.10440184709309364
1104	44-45	0.1358870699693071
1104	46-47	0.012802470124775311
1104	48-49	0.01946937725104192
1104	50-51	-0.049851705468093144
1104	52-53	-0.10113650207361502
1104	54-55	-0.021307415135090935
1104	56-57	-0.04588162587754141
1104	58-59	-0.06634900948390765
1104	60-61	-0.07094924662268909
1104	62-63	2.0558498463429942E-4
1104	64-65	0.06570729868622038
1104	66-67	0.08001033873075158
1104	68-69	0.07528745772156498
1104	70-71	0.05438148233953655
1104	72-73	0.049693515726296766
1104	74-75	0.08202398917507736
1104	76-77	0.15909859632979106
1104	78-79	0.14714344122732115
1104	80-81	0.018787952969208987
1104	82-83	0.021674875428857376
1104	84-85	-0.004818760490717722
1104	86-87	0.050473385272955085
1104	88-89	-0.011707974621497641
1104	90-91	-0.007065521657157348
1104	92-93	-0.06869142280197593
1104	94-95	-0.08079791903583455
1104	96-97	-0.0670026104773136
1104	98-99	-0.2299816147168876
1104	100	-0.16003795951652933
1105	1	-0.11551096681340667
1105	2	-0.0453216920728714
1105	3	-0.005827152088375698
1105	4	0.0337910724699384
1105	5	-0.05046794359709139
1105	6	0.0561670668857559
1105	7	0.008035597531353744
1105	8	-0.001721258605819287
1105	9	0.015414044206735866
1105	10-11	-0.046036519824738775
1105	12-13	-0.009791034543439991
1105	14-15	0.0334900337570474
1105	16-17	-0.04846647511478608
1105	18-19	0.08270689183694913
1105	20-21	0.08028373603168859
1105	22-23	0.04905206640273008
1105	24-25	-0.013443623843627961
1105	26-27	0.07784716824517801
1105	28-29	0.15757476036777263
1105	30-31	0.2060725166097015
1105	32-33	0.16503961692866653
1105	34-35	0.08103007271422058
1105	36-37	0.12502892405408517
1105	38-39	0.10106921947159009
1105	40-41	0.012961155578487649
1105	42-43	-0.05618639106552337
1105	44-45	-0.13601867317788674
1105	46-47	-0.15669710640338508
1105	48-49	-0.20980359595580467
1105	50-51	-0.1770859080580749
1105	52-53	-0.13943899619351896
1105	54-55	-0.2714792199642133
1105	56-57	-0.2994725209895037
1105	58-59	-0.18719626032569892
1105	60-61	-0.253175715841472
1105	62-63	-0.1523714874520934
1105	64-65	-0.1003282369878633
1105	66-67	-0.12330150698090137
1105	68-69	-0.030174710941224703
1105	70-71	-0.12542769499383155
1105	72-73	-0.11681426315734456
1105	74-75	-0.09752574844469564
1105	76-77	-0.13015866665239884
1105	78-79	-0.17542609075107407
1105	80-81	-0.23051525698854292
1105	82-83	-0.275847074496955
1105	84-85	-0.30221851978040704
1105	86-87	-0.2947193196988387
1105	88-89	-0.2862378197405562
1105	90-91	-0.24742124549432987
1105	92-93	-0.35624490929938446
1105	94-95	-0.4836123117592379
1105	96-97	-0.46351841678380623
1105	98-99	-0.4861679932703744
1105	100	-0.3184463946410574
1106	1	-0.13246232191417917
1106	2	-0.17537896754176074
1106	3	-0.11661914636796666
1106	4	-0.009930391487053214
1106	5	-0.009827337530431635
1106	6	-0.012293103613309597
1106	7	-0.035883326209351196
1106	8	-0.02880272164936315
1106	9	0.060569607120548596
1106	10-11	0.0645307185499604
1106	12-13	-0.07837541131411285
1106	14-15	-0.025930981615843507
1106	16-17	-0.09670064800305767
1106	18-19	-0.10436730651344561
1106	20-21	-0.09991494404920331
1106	22-23	-0.04500708447024948
1106	24-25	-0.02736378929023431
1106	26-27	-0.013811922112481057
1106	28-29	-0.115505091312599
1106	30-31	-0.045882527927567196
1106	32-33	-0.1018920421474192
1106	34-35	-0.013977555927233709
1106	36-37	-0.028257957108948517
1106	38-39	-0.050549672441484006
1106	40-41	-0.08645349426759452
1106	42-43	-0.17088101704801062
1106	44-45	-0.18831529388239687
1106	46-47	-0.1778651234846933
1106	48-49	-0.1960152725584976
1106	50-51	-0.16371341341382362
1106	52-53	-0.2614616851575988
1106	54-55	-0.20223177937698011
1106	56-57	-0.2770220857501755
1106	58-59	-0.19264003459783652
1106	60-61	-0.28229268453763723
1106	62-63	-0.32540431130539815
1106	64-65	-0.23077115491109623
1106	66-67	-0.13837149923094927
1106	68-69	-0.15903459656155405
1106	70-71	-0.2172238011753933
1106	72-73	-0.29292633924679734
1106	74-75	-0.2512663517995861
1106	76-77	-0.24082674011000904
1106	78-79	-0.2117204050225574
1106	80-81	-0.2099768347543005
1106	82-83	-0.2600257788125262
1106	84-85	-0.3280305169430413
1106	86-87	-0.2657554947308469
1106	88-89	-0.2517776620867238
1106	90-91	-0.2732176499707535
1106	92-93	-0.2677471379473957
1106	94-95	-0.29871298984484085
1106	96-97	-0.29309274715464895
1106	98-99	-0.3355254818274105
1106	100	-0.2940022721203661
1107	1	-0.47015700780487535
1107	2	-0.37503751757886405
1107	3	-0.3485147877804877
1107	4	-0.23448670831136553
1107	5	-0.20248173546424653
1107	6	-0.20962422264101832
1107	7	-0.16242214904771402
1107	8	-0.19742727017538897
1107	9	-0.2596288508618301
1107	10-11	-0.2231483016702427
1107	12-13	-0.28687329490725233
1107	14-15	-0.21223475774304035
1107	16-17	-0.2354046066255151
1107	18-19	-0.30487808023502794
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2216	58-59	0.5260550668801898
2216	60-61	0.5106148725644886
2216	62-63	0.4689744687072519
2216	64-65	0.3981009870812926
2216	66-67	0.4007717832975004
2216	68-69	0.41433698039277544
2216	70-71	0.3970209057394527
2216	72-73	0.3356148925471203
2216	74-75	0.3959211906932225
2216	76-77	0.4761974427744562
2216	78-79	0.41831517781403704
2216	80-81	0.4241308320928674
2216	82-83	0.3791884153902103
2216	84-85	0.3690060056894673
2216	86-87	0.3373169558264095
2216	88-89	0.4154355310100222
2216	90-91	0.41392591677369595
2216	92-93	0.47438324822508093
2216	94-95	0.4469769390332772
2216	96-97	0.3449621391600459
2216	98-99	0.3296709928868893
2216	100	0.3414579114726628
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63992.0
3	13880.0
4	10493.0
5	9637.0
6	5848.0
7	5087.0
8	6084.0
9	6261.0
10	6191.0
11	6703.0
12	8046.0
13	9570.0
14	10941.0
15	11996.0
16	12027.0
17	11233.0
18	10254.0
19	8647.0
20	7633.0
21	7729.0
22	9223.0
23	15954.0
24	29321.0
25	39018.0
26	39671.0
27	25535.0
28	22969.0
29	28460.0
30	32154.0
31	34863.0
32	44254.0
33	59294.0
34	83387.0
35	143450.0
36	228775.0
37	442870.0
38	693948.0
39	334851.0
40	1334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16377971813375	22.188777776770415	13.85045471262501	20.796987792470823
2	15.309196144419507	23.6619421852126	39.46451672409675	21.564344946271145
3	17.594226060547047	30.42518072141917	29.90252061881884	22.078072599214945
4	11.490180589487858	20.8641286224129	38.04137625477253	29.604314533326708
5	11.52833721197489	42.112506756247974	33.1849554176337	13.174200614143434
6	26.54450663794784	41.02123788916606	18.90107164022185	13.533183832664255
7	24.259320731774068	37.44992669894827	21.863047725029112	16.427704844248556
8	24.016769547082063	37.53367335448425	20.607659846557382	17.841897251876297
9	25.212508420219237	20.05722796343489	20.778404239026745	33.951859377319124
10-11	21.607937607322565	31.174839751982258	28.047645804138117	19.169576836557063
12-13	22.73388691322412	29.95698425551695	27.370487643678672	19.938641187580263
14-15	21.08995973356785	30.787273421339496	25.636564196245352	22.486202648847296
16-17	19.430875070723268	33.59887614481132	26.299959564206883	20.670289220258528
18-19	19.653524456952397	32.094593197865514	28.55559722685692	19.696285118325168
20-21	22.491842899350445	30.789257689567318	27.377932899246098	19.34096651183614
22-23	21.72256742800061	30.939784833616763	27.64765569598722	19.689992042395406
24-25	20.8943870030765	31.084999071755192	27.867365924733615	20.15324800043469
26-27	20.459358705164437	31.043450269773814	27.948882267182885	20.54830875787886
28-29	20.39162661419831	31.080157633442983	28.12052418218841	20.407691570170293
30-31	20.807588912510774	31.69905892665109	27.53658428058233	19.95676788025581
32-33	20.201135440095257	31.322687696485158	28.213556137267133	20.26262072615245
34-35	20.58098962081185	30.831537974039332	28.181546311804052	20.40592609334477
36-37	20.81034984521465	31.14554548594251	27.651110158101194	20.392994510741644
38-39	20.622439922598144	31.18287487881349	27.920801709330355	20.27388348925801
40-41	20.377965548429827	30.896318075556884	28.19105434153022	20.53466203448307
42-43	20.22780181545261	30.842887587161727	28.268929078271114	20.660381519114544
44-45	20.64800135946269	30.993228875507363	27.98522076598949	20.373548999040462
46-47	20.548039604833733	31.384200279741425	27.76573466777555	20.302025447649292
48-49	20.419538450996747	30.95192808272314	27.941900367100082	20.68663309918003
50-51	20.465364366287783	30.861262342585217	27.879706839666152	20.793666451460847
52-53	20.4564924408514	30.951506661324352	27.871989163103756	20.720011734720497
54-55	20.657543589660794	31.461226791461517	27.62432853788324	20.256901080994446
56-57	20.630847041771187	31.056111322103547	27.435638611104057	20.87740302502121
58-59	20.488215866100074	30.679072800583135	27.952727998743004	20.87998333457379
60-61	20.748353363886647	31.25364860855237	27.42647112389055	20.571526903670435
62-63	20.53698447179412	31.1194703628498	27.650558077505977	20.692987087850106
64-65	20.512296005848107	30.865948542413047	27.84243188611864	20.779323565620206
66-67	20.32726039905829	31.007028979429673	27.550992684364527	21.11471793714751
68-69	21.015714102056513	31.386052826331383	27.261172404275136	20.337060667336964
70-71	20.878956678206414	31.06386497215794	27.346228845300118	20.710949504335527
72-73	20.554346488702524	31.22837583113093	27.7006501400188	20.516627540147745
74-75	20.555234651638127	31.41954505668187	27.479356821925098	20.545863469754906
76-77	20.72231476845602	31.294137613372964	27.270545444379103	20.713002173791917
78-79	20.871712032035816	31.200781995209987	27.569859321581745	20.357646651172452
80-81	20.721917157931856	31.151709634363232	27.539820193101132	20.586553014603773
82-83	20.524304039873616	31.1419698211031	27.847048749450416	20.48667738957287
84-85	20.60501978540753	31.095213914151593	27.515725840035614	20.78404046040526
86-87	20.948820575099095	31.22022494563801	27.225337060112825	20.60561741915007
88-89	20.70212613886936	30.962439978806145	27.51737555354766	20.81805832877684
90-91	20.608739755760638	30.880287901913622	27.457357344586047	21.053614997739693
92-93	20.746376908605093	31.191129440615008	27.25906235505075	20.80343129572915
94-95	20.596377227171327	31.061909461588634	27.48776472038161	20.853948590858433
96-97	20.55109433941463	31.2135923190215	27.3775917386445	20.85772160291937
98-99	20.56862594533079	31.144359714978215	27.30304336954189	20.98397097014911
100	20.71260102840945	31.36585801932137	26.992617143334225	20.92892380893496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31329.0
1	21846.0
2	10718.0
3	7415.5
4	4570.5
5	2925.5
6	2443.5
7	2699.0
8	3458.5
9	3638.5
10	2496.0
11	1425.0
12	1231.0
13	1289.5
14	1283.5
15	1341.0
16	1573.5
17	1898.0
18	2320.0
19	2930.5
20	3809.5
21	5152.0
22	7533.0
23	11149.0
24	14926.0
25	18401.5
26	23381.5
27	30461.5
28	40335.5
29	49079.5
30	53706.0
31	59103.0
32	65363.0
33	71132.5
34	77049.0
35	82164.0
36	85090.5
37	87101.5
38	87841.0
39	87166.0
40	86853.5
41	84628.0
42	81013.0
43	78852.0
44	77750.0
45	76516.5
46	75346.0
47	74100.0
48	72680.5
49	71986.5
50	72208.5
51	69957.5
52	65073.5
53	60721.5
54	58124.5
55	56885.0
56	53067.5
57	47893.5
58	43333.0
59	38215.0
60	32289.0
61	25967.0
62	20505.0
63	15864.5
64	11997.0
65	9093.0
66	6937.5
67	5121.5
68	3690.5
69	2657.0
70	1970.0
71	1482.5
72	1115.5
73	888.5
74	745.5
75	576.0
76	408.5
77	331.5
78	273.0
79	212.0
80	160.5
81	122.5
82	104.5
83	90.5
84	77.0
85	75.5
86	71.0
87	60.0
88	56.0
89	50.5
90	39.5
91	31.0
92	32.0
93	33.0
94	25.0
95	20.5
96	17.5
97	12.5
98	12.5
99	14.5
100	20.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1851601934699752
2	0.1803993810156898
3	0.19452443614865225
4	0.18370440784345818
5	0.19838030078104868
6	0.07939933498138758
7	0.08089446616537803
8	0.06850061556124667
9	0.06161514300339592
10-11	0.07577954369383176
12-13	0.07599594425993564
14-15	0.07375324748394996
16-17	0.06901210780840131
18-19	0.07339913746668907
20-21	0.07572052535762161
22-23	0.07373357470521325
24-25	0.07253353520227354
26-27	0.07694023763929803
28-29	0.0745008130759452
30-31	0.08010755501590938
32-33	0.07676318263066759
34-35	0.0760746353748825
36-37	0.08892095988995834
38-39	0.08297978071146998
40-41	0.07711729264792848
42-43	0.08069773837801086
44-45	0.09224565949646342
46-47	0.08242894290684193
48-49	0.08738648314849447
50-51	0.08473065801903774
52-53	0.08303879904768013
54-55	0.08506509525756192
56-57	0.07859275105318221
58-59	0.08632415309671178
60-61	0.08213385122579117
62-63	0.07404833916500071
64-65	0.07701892875424489
66-67	0.07688121930308789
68-69	0.07560248868520131
70-71	0.07123513180565025
72-73	0.06867767056987711
74-75	0.07404833916500071
76-77	0.07914358885781028
78-79	0.07575987091509503
80-81	0.08406178354198938
82-83	0.08496673136387833
84-85	0.08701270035249685
86-87	0.0836093096310449
88-89	0.0852814958236658
90-91	0.0829010895965231
92-93	0.07479590475699593
94-95	0.07469754086331236
96-97	0.07296633633448131
98-99	0.0837273463034652
100	0.09136038445331118
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2541583.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.47247975463836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40410404526955	52.78187698584378
2	13.629393905226317	18.119592208662954
3	3.7577181298044224	7.493545269211858
4	1.370054455492854	3.6428366822200333
5	0.6208376884247462	2.063431033736521
6	0.32443325634606685	1.2939529838517194
7	0.19524954157128274	0.9085104839439658
8	0.12039388843042163	0.6402304249018714
9	0.08652204198912773	0.5176201216007037
>10	0.4283783957036171	5.204610600418031
>50	0.038200311324696874	1.7781731262580844
>100	0.022644785199471804	2.9478329080511023
>500	0.0014782537267932555	0.6909474533133411
>1k	5.321713416302933E-4	0.591151356596752
>5k	0.0	0.0
>10k+	5.913014907003259E-5	1.325688361389312
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33728	1.3270469624639447	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2806	0.11040363427045272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.93455574734329E-5	0.0
5	0.0	0.0	0.0	3.93455574734329E-5	0.0
6	0.0	0.0	0.0	3.93455574734329E-5	0.0
7	0.0	0.0	0.0	3.93455574734329E-5	0.0
8	0.0	0.0	0.0	3.93455574734329E-5	0.0
9	0.0	0.0	0.0	3.93455574734329E-5	0.0
10-11	0.0	0.0	0.0	3.93455574734329E-5	0.0
12-13	0.0	0.0	0.0	3.93455574734329E-5	0.0
14-15	0.0	3.93455574734329E-5	0.0	7.86911149468658E-5	0.0
16-17	0.0	3.93455574734329E-5	0.0	9.836389368358224E-5	0.0
18-19	0.0	3.93455574734329E-5	0.0	1.7705500863044805E-4	0.0
20-21	0.0	3.93455574734329E-5	0.0	2.557461235773138E-4	0.0
22-23	0.0	3.93455574734329E-5	0.0	5.901833621014934E-4	0.0
24-25	0.0	3.93455574734329E-5	0.0	0.0012984033966232855	0.0
26-27	0.0	3.93455574734329E-5	0.0	0.00210498732482866	0.0
28-29	0.0	3.93455574734329E-5	0.0	0.004347684100814335	0.0
30-31	0.0	3.93455574734329E-5	0.0	0.010308536058039419	0.0
32-33	0.0	3.93455574734329E-5	0.0	0.01916128648956182	0.0
34-35	0.0	5.9018336210149344E-5	0.0	0.03366012441852184	0.0
36-37	0.0	7.86911149468658E-5	0.0	0.05577232771859113	0.0
38-39	0.0	7.86911149468658E-5	0.0	0.0886258682089076	0.0
40-41	0.0	7.86911149468658E-5	0.0	0.12279748487458406	0.0
42-43	0.0	7.86911149468658E-5	0.0	0.15513953311774592	0.0
44-45	0.0	7.86911149468658E-5	0.0	0.1947211639360194	0.0
46-47	0.0	7.86911149468658E-5	0.0	0.244493294139912	0.0
48-49	0.0	7.86911149468658E-5	0.0	0.30012791240734615	0.0
50-51	0.0	7.86911149468658E-5	0.0	0.36180207374695217	0.0
52-53	0.0	1.1803667242029869E-4	0.0	0.41989578935647587	0.0
54-55	0.0	1.1803667242029869E-4	0.0	0.48507170531121746	0.0
56-57	0.0	1.1803667242029869E-4	0.0	0.5633103463471387	0.0
58-59	0.0	1.1803667242029869E-4	0.0	0.6540805474383484	0.0
60-61	0.0	1.1803667242029869E-4	0.0	0.7542543367657086	0.0
62-63	0.0	1.1803667242029869E-4	0.0	0.8552740555787476	0.0
64-65	0.0	1.1803667242029869E-4	0.0	0.9615070607570164	0.0
66-67	0.0	1.1803667242029869E-4	0.0	1.0885932113962047	0.0
68-69	0.0	1.1803667242029869E-4	0.0	1.2343685018352737	0.0
70-71	0.0	1.1803667242029869E-4	0.0	1.4010166105139985	0.0
72-73	0.0	1.1803667242029869E-4	0.0	1.582281593794104	0.0
74-75	0.0	1.1803667242029869E-4	0.0	1.7645695615685186	0.0
76-77	0.0	1.1803667242029869E-4	0.0	1.957618539311917	0.0
78-79	0.0	1.1803667242029869E-4	0.0	2.1822029813702724	0.0
80-81	0.0	1.1803667242029869E-4	0.0	2.4361588820825446	0.0
82-83	0.0	1.1803667242029869E-4	0.0	2.7090399959395386	0.0
84-85	0.0	1.1803667242029869E-4	0.0	2.9861704300036633	0.0
86-87	0.0	1.1803667242029869E-4	0.0	3.280081744330207	0.0
88	0.0	1.1803667242029869E-4	0.0	3.5167846180903792	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5280	0.0	50.346203	1
GTATCAA	8995	0.0	39.85713	1
ATCAACG	10935	0.0	31.88119	3
TCAACGC	10975	0.0	31.634518	4
TATCAAC	11250	0.0	31.615812	2
CAACGCA	11650	0.0	29.761236	5
GTACATG	19660	0.0	28.502337	1
AACGCAG	12640	0.0	27.588606	6
TACATGG	19715	0.0	27.371698	2
CATGGGT	9900	0.0	25.993307	4
TACCTGG	1975	0.0	25.250725	2
CGCAGAG	13560	0.0	25.194448	8
ACATGGG	20350	0.0	25.130445	3
ACGCAGA	14205	0.0	24.41628	7
ATGGGTG	4620	0.0	23.827763	5
TGGTATC	2455	0.0	23.57158	2
GTGGTAT	2535	0.0	23.571	1
GTACCTG	2300	0.0	22.70635	1
GCAGAGT	14470	0.0	22.636616	9
ATGGGTA	4180	0.0	22.621906	5
>>END_MODULE