##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431413_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2541583
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.643838505372436	34.0	31.0	34.0	31.0	34.0
2	32.74956906778177	34.0	31.0	34.0	31.0	34.0
3	32.82619926242818	34.0	31.0	34.0	31.0	34.0
4	36.304917447118584	37.0	37.0	37.0	35.0	37.0
5	36.228910486102556	37.0	37.0	37.0	35.0	37.0
6	36.28258608906339	37.0	37.0	37.0	35.0	37.0
7	36.237873797550584	37.0	37.0	37.0	35.0	37.0
8	36.25577602620099	37.0	37.0	37.0	35.0	37.0
9	38.045350082999455	39.0	39.0	39.0	35.0	39.0
10-11	38.02296993645299	39.0	39.0	39.0	35.0	39.0
12-13	38.00774143516068	39.0	39.0	39.0	35.0	39.0
14-15	39.42884021493691	41.0	39.0	41.0	36.0	41.0
16-17	39.46170949365022	41.0	39.0	41.0	36.5	41.0
18-19	39.56427057467728	41.0	39.5	41.0	37.0	41.0
20-21	39.56163678306001	41.0	40.0	41.0	37.0	41.0
22-23	39.470381647972935	41.0	39.5	41.0	36.0	41.0
24-25	39.40254105413831	41.0	39.0	41.0	36.0	41.0
26-27	39.26627066674588	41.0	39.0	41.0	35.5	41.0
28-29	39.16168801097584	41.0	39.0	41.0	35.0	41.0
30-31	38.89837613015196	41.0	38.5	41.0	35.0	41.0
32-33	38.56338116835059	41.0	38.0	41.0	35.0	41.0
34-35	38.37563184047108	40.0	38.0	41.0	35.0	41.0
36-37	38.200550798459076	40.0	38.0	41.0	35.0	41.0
38-39	38.02018289388936	40.0	37.5	41.0	34.0	41.0
40-41	37.83631795617141	40.0	37.0	41.0	34.0	41.0
42-43	37.5897177074288	40.0	37.0	41.0	33.0	41.0
44-45	37.22002822650293	40.0	37.0	41.0	33.0	41.0
46-47	36.642534396869976	40.0	37.0	41.0	30.5	41.0
48-49	36.12146504757075	40.0	36.0	41.0	28.0	41.0
50-51	35.882393571250674	40.0	36.0	41.0	27.0	41.0
52-53	35.790644059233955	40.0	36.0	41.0	27.0	41.0
54-55	35.63538609598821	40.0	35.0	41.0	26.0	41.0
56-57	35.475957503650285	40.0	35.0	41.0	26.0	41.0
58-59	35.284959806545764	40.0	35.0	41.0	25.0	41.0
60-61	35.12072967910157	39.0	35.0	41.0	25.0	41.0
62-63	34.94626577215853	39.0	35.0	41.0	25.0	41.0
64-65	34.69948768149614	39.0	35.0	41.0	23.5	41.0
66-67	34.428730244103775	38.0	35.0	41.0	23.0	41.0
68-69	34.12428277966921	37.0	35.0	40.5	22.0	41.0
70-71	33.81129536198503	37.0	35.0	40.0	22.0	41.0
72-73	33.48556608224087	36.0	35.0	39.0	21.0	41.0
74-75	33.14701703623293	36.0	34.0	39.0	20.0	41.0
76-77	32.81836064374053	35.5	34.0	38.5	19.5	40.5
78-79	32.50161080712296	35.0	34.0	37.0	18.5	39.5
80-81	32.241302172701026	35.0	34.0	37.0	18.0	39.0
82-83	32.047074008600156	35.0	34.0	36.5	18.0	39.0
84-85	31.792867476686773	35.0	34.0	36.0	16.0	37.5
86-87	31.565854823548946	35.0	34.0	36.0	11.5	37.0
88-89	31.348635279666254	35.0	34.0	36.0	7.0	37.0
90-91	31.17452312200703	35.0	34.0	35.0	2.0	36.0
92-93	31.019275388606236	35.0	34.0	35.0	2.0	36.0
94-95	30.888474033702618	35.0	34.0	35.0	2.0	36.0
96-97	30.75465684181866	35.0	34.0	35.0	2.0	36.0
98-99	30.643176713095734	35.0	34.0	35.0	2.0	36.0
100	30.544564942400072	35.0	34.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.014378731509758325
1101	2	-0.020279329284093706
1101	3	-0.017478451034428133
1101	4	0.05466051188628995
1101	5	0.08255252325707829
1101	6	0.029885869018968947
1101	7	0.05130182631508262
1101	8	0.0613828005213648
1101	9	0.1114435598681851
1101	10-11	0.11450596702243132
1101	12-13	0.06234718793026417
1101	14-15	0.07326373110115725
1101	16-17	-0.008494552102163766
1101	18-19	0.029466660751019447
1101	20-21	0.031928515422897874
1101	22-23	0.037142576231374846
1101	24-25	0.06668552560094554
1101	26-27	0.03991379340449441
1101	28-29	0.013255714056420231
1101	30-31	0.047341947298228604
1101	32-33	0.04255919539960473
1101	34-35	0.04784964121621016
1101	36-37	0.053148574795322645
1101	38-39	0.06877357760852476
1101	40-41	-0.023775551655440097
1101	42-43	-0.04239964221915926
1101	44-45	-0.02587017183162743
1101	46-47	0.07965815700705292
1101	48-49	0.12436098735047807
1101	50-51	-0.019560509848290053
1101	52-53	-0.023346900976044083
1101	54-55	0.012194544677846864
1101	56-57	0.03623566280064949
1101	58-59	0.013773272574781004
1101	60-61	-0.04905018558510221
1101	62-63	-0.024906544074156045
1101	64-65	0.004816183036524535
1101	66-67	-0.09003744252264312
1101	68-69	-0.10302107084245193
1101	70-71	-0.10658312417994154
1101	72-73	-0.167232840976979
1101	74-75	-0.17678640073260254
1101	76-77	-0.14423632097435046
1101	78-79	-0.1962962767082388
1101	80-81	-0.2041627498812062
1101	82-83	-0.16164765447171447
1101	84-85	-0.1591153341794751
1101	86-87	-0.2016222196006474
1101	88-89	-0.18922990667488904
1101	90-91	-0.19871619267429352
1101	92-93	-0.2688404618360458
1101	94-95	-0.31325871415026896
1101	96-97	-0.2570173209920412
1101	98-99	-0.25125970461966673
1101	100	-0.20047344042119164
1102	1	-0.04515424365339271
1102	2	-0.013343099951789839
1102	3	-0.004541994110518033
1102	4	-0.016868434764738538
1102	5	0.014593607293406308
1102	6	-0.03972371133712471
1102	7	-0.06002032460066431
1102	8	-0.03138782090375969
1102	9	0.03202362459423824
1102	10-11	0.021493529516341425
1102	12-13	-0.08515218056555796
1102	14-15	-0.08949107458312966
1102	16-17	-0.04423002938177234
1102	18-19	-0.043953828495908454
1102	20-21	-0.03354439992553182
1102	22-23	-0.06291253970278632
1102	24-25	-0.044858824124823116
1102	26-27	0.02234367455665165
1102	28-29	-0.030006386439026755
1102	30-31	0.031313386427477496
1102	32-33	0.06007100952978561
1102	34-35	0.05436304411087178
1102	36-37	0.026412969964383137
1102	38-39	0.0364203992042178
1102	40-41	0.0686661873945269
1102	42-43	0.06618007674271809
1102	44-45	0.09443168716661887
1102	46-47	0.10651599853826355
1102	48-49	0.05783108406441073
1102	50-51	0.11037710254069566
1102	52-53	0.07944970903448478
1102	54-55	0.15518045588211038
1102	56-57	0.19998969880014528
1102	58-59	0.09133511621685386
1102	60-61	0.0600267647624122
1102	62-63	0.04840124356037734
1102	64-65	0.06945266797171001
1102	66-67	0.09411410034836365
1102	68-69	0.04132460641764624
1102	70-71	0.0336292488001817
1102	72-73	0.06195550108069625
1102	74-75	0.06474456548129837
1102	76-77	0.10099425933422168
1102	78-79	0.1686042676358639
1102	80-81	0.1514964967155521
1102	82-83	0.09914192270809963
1102	84-85	0.10934154784053973
1102	86-87	0.05982004215971415
1102	88-89	0.07561599067378921
1102	90-91	0.07092288392903612
1102	92-93	0.06398709500557942
1102	94-95	0.10198295187030482
1102	96-97	0.17283671088078734
1102	98-99	0.10926759511847806
1102	100	0.11078371558409827
1103	1	-0.08634861554997997
1103	2	-0.07140065245037164
1103	3	-0.045547335489494856
1103	4	0.011400303672310486
1103	5	-0.03536296636185199
1103	6	-0.008930778222293156
1103	7	-0.04960491199510386
1103	8	-0.02842429534793922
1103	9	-0.008588146340699154
1103	10-11	-0.02633664276401504
1103	12-13	0.011247471409447485
1103	14-15	-0.031359967561449764
1103	16-17	0.019735456194780454
1103	18-19	-0.014407176194126237
1103	20-21	-0.059990475707266455
1103	22-23	-0.05978977397901275
1103	24-25	-0.03827461939816601
1103	26-27	-0.04628622733788745
1103	28-29	-0.005234635798380793
1103	30-31	-0.02613484592988158
1103	32-33	-0.03551178798674215
1103	34-35	-0.016488158313634926
1103	36-37	0.0057775479487602865
1103	38-39	0.027088802515187638
1103	40-41	0.11910178680093253
1103	42-43	0.13704663501756187
1103	44-45	0.13244110214557736
1103	46-47	0.11225394235305686
1103	48-49	0.013267024370684055
1103	50-51	0.013619889590671619
1103	52-53	0.07039136970213633
1103	54-55	0.04440573173460649
1103	56-57	0.07844084938329132
1103	58-59	0.1345630633730721
1103	60-61	0.13458901553696023
1103	62-63	0.06696861643236218
1103	64-65	0.0023585814482558476
1103	66-67	-0.04544132628629427
1103	68-69	-0.025256901821691713
1103	70-71	0.0418551474859683
1103	72-73	-0.02867065828600346
1103	74-75	-0.005388290264612294
1103	76-77	0.0018937436233485982
1103	78-79	0.0330101129324305
1103	80-81	0.06927428711924932
1103	82-83	0.061113773591742415
1103	84-85	0.019175296191320967
1103	86-87	0.06029415403875049
1103	88-89	0.044788583674964855
1103	90-91	0.07105018392829976
1103	92-93	0.040451259979008114
1103	94-95	0.026126126133409144
1103	96-97	-0.045391958000394084
1103	98-99	-0.04893075517180634
1103	100	0.005821871801007461
1104	1	-0.0591266157240824
1104	2	-0.07906905993874602
1104	3	-0.06209060795639232
1104	4	-0.02272097166606102
1104	5	-0.035153460850033014
1104	6	-0.06524507817656655
1104	7	-0.06760269218688819
1104	8	-0.0794139062040955
1104	9	-0.010150855156545902
1104	10-11	-0.08951056237766153
1104	12-13	-0.11159934986932285
1104	14-15	-0.12495299168692497
1104	16-17	-0.0791363175429396
1104	18-19	-0.05193617286042951
1104	20-21	-0.024564771848439193
1104	22-23	-0.038118597400959686
1104	24-25	-0.027609723694212107
1104	26-27	-0.08801491294904906
1104	28-29	0.007589645629899167
1104	30-31	0.039108955956074
1104	32-33	0.06291411602877872
1104	34-35	0.10292473429080928
1104	36-37	0.06565210058476367
1104	38-39	-0.0011148233554791886
1104	40-41	-0.08087110928555319
1104	42-43	-0.047375802578308424
1104	44-45	0.08606911844256615
1104	46-47	-0.060091889483445016
1104	48-49	0.013405953958447014
1104	50-51	-0.05599080717530569
1104	52-53	0.018937727892499367
1104	54-55	0.05515905807522614
1104	56-57	-0.009183681489879802
1104	58-59	-0.017608437501735352
1104	60-61	0.03244699409536622
1104	62-63	0.02410918344286017
1104	64-65	-0.004914761787681243
1104	66-67	-0.026948939903576274
1104	68-69	-0.04689202414103022
1104	70-71	-0.05827871688074282
1104	72-73	-0.03047409920330324
1104	74-75	0.004140264095248369
1104	76-77	-0.008587968859693262
1104	78-79	-0.006732714837184517
1104	80-81	-0.04597937700494725
1104	82-83	0.04854941999670359
1104	84-85	-0.04695806119351431
1104	86-87	-0.056976786090796594
1104	88-89	0.04312675438379543
1104	90-91	0.008571793494624558
1104	92-93	0.007104902156470416
1104	94-95	0.06043728978863072
1104	96-97	0.07881182143612264
1104	98-99	0.1316418079127999
1104	100	0.2105758844369312
1105	1	-0.08807493343022088
1105	2	-0.11643040147355777
1105	3	-0.08701112972764236
1105	4	0.01452424755974846
1105	5	-0.0365741236537005
1105	6	0.02237677064792365
1105	7	0.0016445618014415686
1105	8	-0.0019683422823248975
1105	9	0.03664793372523434
1105	10-11	-0.005888265545813454
1105	12-13	-0.005136169444092786
1105	14-15	-0.060419441891617964
1105	16-17	-0.09814119430532742
1105	18-19	-0.10864495196920387
1105	20-21	-0.015200593932583217
1105	22-23	-0.027441750428813805
1105	24-25	-0.016329452762136043
1105	26-27	-0.008260987359470562
1105	28-29	-0.06214835621981507
1105	30-31	-0.03951056705094658
1105	32-33	-0.055800117831815044
1105	34-35	0.04208454818046192
1105	36-37	0.07946889479243424
1105	38-39	0.10015956279764282
1105	40-41	0.05860702956600505
1105	42-43	0.02619509702122258
1105	44-45	0.025463199725933805
1105	46-47	0.0683211792958005
1105	48-49	0.004498473846261675
1105	50-51	-0.016022707097356204
1105	52-53	-0.13672493904736172
1105	54-55	-0.10877331528871537
1105	56-57	-0.04752940307577802
1105	58-59	-0.061116732193184475
1105	60-61	-0.03126879243286851
1105	62-63	-0.03745905208614886
1105	64-65	-0.03616473603474901
1105	66-67	-0.11372500627159354
1105	68-69	-0.06910198900841635
1105	70-71	-0.10405836393635326
1105	72-73	-0.03611573302171678
1105	74-75	-0.13271407245492384
1105	76-77	-0.1883167970469941
1105	78-79	-0.02619155491017011
1105	80-81	-0.037908427023324975
1105	82-83	-0.04469477135938149
1105	84-85	-0.1414332988602922
1105	86-87	-0.17369195567244589
1105	88-89	-0.11964121189129173
1105	90-91	-0.09075922962907157
1105	92-93	-0.04973048772792765
1105	94-95	-0.08693416937058984
1105	96-97	-0.03542147727278433
1105	98-99	-0.06022363704728306
1105	100	-0.0749355350683949
1106	1	-0.18690202088770747
1106	2	-0.13182998100624843
1106	3	-0.1143974529793681
1106	4	-0.07198276236961476
1106	5	-0.11273051579869531
1106	6	-0.11876016096833553
1106	7	-0.09423033418784854
1106	8	-0.07921151254303993
1106	9	-0.14034118636200077
1106	10-11	-0.13372206677866671
1106	12-13	-0.11494275590893466
1106	14-15	-0.15607675389805564
1106	16-17	-0.1473698023530119
1106	18-19	-0.1577655530119486
1106	20-21	-0.08796541626159637
1106	22-23	-0.12149298007447129
1106	24-25	-0.10333332345916091
1106	26-27	-0.11037952317412447
1106	28-29	-0.12196771655573713
1106	30-31	-0.12946666489737169
1106	32-33	-0.12400934242215556
1106	34-35	-0.09640273289751633
1106	36-37	-0.09546505929870364
1106	38-39	-0.14655241424078724
1106	40-41	-0.17830307092653186
1106	42-43	-0.18640161688259838
1106	44-45	-0.18297658253308668
1106	46-47	-0.35082828070486016
1106	48-49	-0.33791295470070537
1106	50-51	-0.38978731676638745
1106	52-53	-0.3112689689396504
1106	54-55	-0.3145627690665762
1106	56-57	-0.3121164859089802
1106	58-59	-0.3212258239067225
1106	60-61	-0.3525056385569627
1106	62-63	-0.3714531418514895
1106	64-65	-0.2946059812231354
1106	66-67	-0.26907661599343413
1106	68-69	-0.2588002901549231
1106	70-71	-0.24662789589886103
1106	72-73	-0.23474261324276569
1106	74-75	-0.3115140850147142
1106	76-77	-0.31032917414308514
1106	78-79	-0.3323749291817393
1106	80-81	-0.318876607952717
1106	82-83	-0.27829590402352267
1106	84-85	-0.22434714017027346
1106	86-87	-0.16838730241524758
1106	88-89	-0.23712039904491533
1106	90-91	-0.2717565977834546
1106	92-93	-0.19172248372021272
1106	94-95	-0.22794368340686333
1106	96-97	-0.19824894152378647
1106	98-99	-0.22365008167219713
1106	100	-0.22038175707113794
1107	1	-0.2533670609402563
1107	2	-0.23817578260656092
1107	3	-0.24007844724220462
1107	4	-0.2106760716990692
1107	5	-0.24630796082031736
1107	6	-0.21150933760984003
1107	7	-0.12666191528277437
1107	8	-0.14583320930049837
1107	9	-0.2068964651604901
1107	10-11	-0.13761116055746214
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2216	34-35	0.07977722334899084
2216	36-37	0.1135829287563368
2216	38-39	0.1290543420422381
2216	40-41	0.11273874386053251
2216	42-43	0.12498698741318037
2216	44-45	0.15007270814214735
2216	46-47	0.16313580122379534
2216	48-49	0.3077735035890683
2216	50-51	0.2863248973178969
2216	52-53	0.3053465220588194
2216	54-55	0.31158465999383367
2216	56-57	0.32732034051496584
2216	58-59	0.3458032747322193
2216	60-61	0.2614572883336166
2216	62-63	0.3287562594358917
2216	64-65	0.3187481420657363
2216	66-67	0.3422414105490077
2216	68-69	0.3657492997353984
2216	70-71	0.4203515251544516
2216	72-73	0.36489231261254673
2216	74-75	0.3741210622137814
2216	76-77	0.388411002285423
2216	78-79	0.39005428450118274
2216	80-81	0.3767779471999688
2216	82-83	0.3659473314494335
2216	84-85	0.34564105107074994
2216	86-87	0.3632679667808816
2216	88-89	0.3620258526430291
2216	90-91	0.3439376271794288
2216	92-93	0.3213012548851424
2216	94-95	0.3288385085992225
2216	96-97	0.31914199058427783
2216	98-99	0.296236033762856
2216	100	0.2638898540197694
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62.0
3	480.0
4	110.0
5	153.0
6	343.0
7	752.0
8	1359.0
9	2227.0
10	3206.0
11	4800.0
12	7749.0
13	11329.0
14	12352.0
15	16406.0
16	22580.0
17	36162.0
18	44202.0
19	22132.0
20	11858.0
21	9399.0
22	8660.0
23	8368.0
24	8675.0
25	9657.0
26	11452.0
27	14115.0
28	17126.0
29	21061.0
30	26529.0
31	33202.0
32	41782.0
33	54328.0
34	76611.0
35	135969.0
36	201643.0
37	420340.0
38	779307.0
39	460981.0
40	4116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.170609325765945	20.28159714680542	14.769075072636214	20.778718454792415
2	15.150356192196233	22.105242448745113	40.73984886669697	22.00455249236169
3	16.821846705414462	29.42066381173167	32.134268505534386	21.623220977319477
4	10.802165422101108	18.93044610386519	39.38391939196949	30.88346908206421
5	10.487936218269937	39.202980301635876	35.04873615087934	15.260347329214843
6	26.067100700626344	40.05181023008102	18.61505211515815	15.26603695413449
7	23.075225164789032	36.7034639435344	23.00495399914148	17.216356892535085
8	25.23883737025311	33.5061652521283	21.269342767873407	19.985654609745186
9	26.66798605436061	15.066909087761445	22.454863760105415	35.81024109777253
10-11	22.48978294236309	27.59646251961868	29.04815620815846	20.86559832985978
12-13	25.105928077107848	26.689449055962367	29.34002155349638	18.8646013134334
14-15	22.37039324908792	26.71253048051755	29.772572796672943	21.14450347372159
16-17	18.15358774433099	30.130237729792807	30.20017445820184	21.51600006767436
18-19	18.023541641296966	26.56312614803027	36.119518496652574	19.29381371402019
20-21	20.488471161476923	25.412961134851784	35.9748235646839	18.123744138987394
22-23	19.664940903921433	25.550213518630034	36.500186989872255	18.28465858757628
24-25	19.02368288727257	25.796787197894854	36.44127948655601	18.73825042827656
26-27	18.662292483866825	25.824649592900794	36.486169328954695	19.02688859427769
28-29	18.57859200492922	25.832371496689948	36.63306091076864	18.955975587612187
30-31	19.064514250595497	26.392402672670283	36.055887326692314	18.487195750041906
32-33	18.496362249232064	26.028322164921075	36.67909612389009	18.796219461956774
34-35	18.881059036692104	25.83300381065922	36.26679336075749	19.019143791891185
36-37	19.21197839736664	26.13193093012038	35.61097947623923	19.04511119627376
38-39	19.06697917924921	26.232465923367265	35.716399159410926	18.9841557379726
40-41	18.912375842316766	26.0949060553558	35.675829549100726	19.316888553226708
42-43	18.926120811949662	26.091243513092248	35.442502571745756	19.540133103212337
44-45	19.536315445095703	26.349270551315186	34.67097396616353	19.44344003742558
46-47	19.69466201496158	26.964715214215424	33.63028151419375	19.71034125662925
48-49	19.931058592130835	27.023186574799873	32.766428422336745	20.279326410732544
50-51	20.050331015347183	27.206309948087288	32.236244154213104	20.507114882352433
52-53	20.106418620169883	27.425998886906953	32.124341424865335	20.34324106805783
54-55	20.262861817145232	27.885433513661965	31.803710589957717	20.047994079235085
56-57	20.109799915956202	27.288914717579793	31.815534882110473	20.78575048435353
58-59	19.888021530083886	26.837316451313363	32.39764160594281	20.87702041265994
60-61	20.13237231627159	27.2577471131224	31.900595051786446	20.709285518819566
62-63	19.862083808520296	27.078033486344026	32.30554582284348	20.754336882292197
64-65	19.81006817313967	26.92306859984887	32.44056220166997	20.82630102534149
66-67	19.742863561839165	27.063105506873903	32.07561943035975	21.118411500927184
68-69	20.51834404224625	27.19070403011991	31.652367459620013	20.638584468013825
70-71	20.4075666555974	26.97814580897177	31.573962885961915	21.04032464946891
72-73	20.157866615045155	27.165644466268173	31.792930842910067	20.883558075776605
74-75	20.33235827774585	27.290139597086753	31.495541427337375	20.881960697830017
76-77	20.46640380900969	27.240942840853606	31.292107620812942	21.000545729323765
78-79	20.67784119479657	27.2103996216488	31.520125088823608	20.591634094731017
80-81	20.494134690823014	27.17471279382614	31.46199136433851	20.86916115101233
82-83	20.224903454014996	27.15583299069263	31.798598091310957	20.820665463981413
84-85	20.18260167150428	27.205736829949366	31.440133021554228	21.171528476992126
86-87	20.368847145608314	27.37768262887449	31.141365174727582	21.112105050789616
88-89	20.096059850315193	27.121877459753247	31.404240853136116	21.37782183679544
90-91	19.931813129415378	27.026257650366215	31.374301851826345	21.66762736839206
92-93	19.923164816347857	27.247157633312664	31.26874844582663	21.560929104512848
94-95	19.745073691019446	26.983566772822027	31.511261989692933	21.76009754646559
96-97	19.60982893498231	27.14558182860841	31.361251034052668	21.883338202356608
98-99	19.66865388822351	26.966499473554162	31.30991370694987	22.054932931272457
100	19.798573877557125	27.176276776190534	30.97694856382474	22.048200782427603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7531.0
1	4155.0
2	4020.0
3	6475.5
4	3438.0
5	1628.5
6	3811.5
7	9664.5
8	17226.5
9	18910.0
10	11558.0
11	4981.5
12	3998.5
13	4274.5
14	4795.5
15	5303.5
16	5861.5
17	6325.5
18	6886.5
19	7560.5
20	8250.5
21	9404.5
22	11473.0
23	14726.0
24	17991.0
25	21025.0
26	25614.5
27	31761.0
28	41140.0
29	49549.5
30	53149.5
31	58068.0
32	64201.5
33	69399.5
34	74949.5
35	79900.0
36	82923.5
37	85024.5
38	85753.0
39	85415.5
40	85195.0
41	82805.0
42	79328.5
43	77757.5
44	77223.0
45	76340.0
46	74863.0
47	73872.0
48	72375.5
49	70222.0
50	70984.5
51	68923.5
52	62413.0
53	57582.0
54	55137.0
55	53610.0
56	49688.0
57	44662.5
58	40491.0
59	36175.5
60	30253.5
61	24082.5
62	19157.0
63	14617.0
64	10860.5
65	8132.0
66	6121.0
67	4392.5
68	3072.0
69	2094.5
70	1409.5
71	978.0
72	721.0
73	511.0
74	393.0
75	293.0
76	167.5
77	112.5
78	92.0
79	53.5
80	25.0
81	14.0
82	9.0
83	4.5
84	1.0
85	1.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005941179178488368
2	0.004642775781865081
3	7.47565591995225E-4
4	0.0
5	0.04599495668644305
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.967277873671645E-5
16-17	0.0
18-19	5.9018336210149344E-5
20-21	0.0
22-23	5.9018336210149344E-5
24-25	3.93455574734329E-5
26-27	1.3770945115701514E-4
28-29	1.7705500863044805E-4
30-31	1.967277873671645E-4
32-33	0.001573822298937316
34-35	0.0011016756092561211
36-37	0.0016131678564107487
38-39	0.0012590578391498527
40-41	2.5574612357731386E-4
42-43	8.459294856788072E-4
44-45	2.3607334484059738E-4
46-47	2.3607334484059738E-4
48-49	1.7705500863044805E-4
50-51	3.541100172608961E-4
52-53	6.492016983116429E-4
54-55	7.672383707319414E-4
56-57	0.0020066234311450775
58-59	9.049478218889567E-4
60-61	0.0015541495202005995
62-63	0.0015541495202005995
64-65	0.0015541495202005995
66-67	5.508378046280606E-4
68-69	3.344372385241796E-4
70-71	4.328011322077619E-4
72-73	2.754189023140303E-4
74-75	7.278928132585086E-4
76-77	0.001180366724202987
78-79	0.001141021166729554
80-81	0.001141021166729554
82-83	0.001888586758724779
84-85	0.001573822298937316
86-87	0.0027345162444035865
88-89	0.001672186192620898
90-91	0.00149513118399045
92-93	0.0015344767414638828
94-95	9.836389368358225E-4
96-97	2.1640056610388095E-4
98-99	4.328011322077619E-4
100	2.3607334484059738E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2541583.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.060167290872435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.51016592738208	45.95213365050975
2	15.298619436825481	18.37675285390262
3	4.337756325563593	7.815791117411684
4	1.6312522140119117	3.9189312346864584
5	0.7296108297437381	2.191027424582058
6	0.3841014463165449	1.3841518273462647
7	0.23246089818284824	0.9773148303413829
8	0.14690845087333215	0.7058676908716195
9	0.10952802246189394	0.5920444216889805
>10	0.5324204579727286	5.8422417824478465
>50	0.049802290516270234	2.0768737834783586
>100	0.03369495138748727	3.93945941883911
>500	0.0022992179762984914	0.9885767219072817
>1k	8.53995248336216E-4	0.7858190680944377
>5k	3.2845971089854464E-4	1.33605417999106
>10k+	1.9707582653912678E-4	3.116959993901123
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34009	1.3381030641139795	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27828	1.0949081733706907	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17164	0.6753271484740022	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9050	0.3560772951345677	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8474	0.3334142540298704	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5774	0.22718124885160157	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5325	0.20951509354603018	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5240	0.20617072116078838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.93455574734329E-5	0.0
5	0.0	0.0	0.0	3.93455574734329E-5	0.0
6	0.0	0.0	0.0	3.93455574734329E-5	0.0
7	0.0	0.0	0.0	3.93455574734329E-5	0.0
8	0.0	0.0	0.0	3.93455574734329E-5	0.0
9	0.0	0.0	0.0	3.93455574734329E-5	0.0
10-11	0.0	0.0	0.0	3.93455574734329E-5	0.0
12-13	0.0	0.0	0.0	3.93455574734329E-5	0.0
14-15	0.0	0.0	0.0	7.86911149468658E-5	0.0
16-17	0.0	0.0	0.0	9.836389368358224E-5	0.0
18-19	0.0	0.0	0.0	2.3607334484059738E-4	0.0
20-21	0.0	0.0	0.0	3.344372385241796E-4	0.0
22-23	0.0	0.0	0.0	6.688744770483592E-4	0.0
24-25	0.0	0.0	0.0	0.0013770945115701514	0.0
26-27	0.0	0.0	0.0	0.0021246601035653763	0.0
28-29	0.0	0.0	0.0	0.004465720773234634	0.0
30-31	0.0	0.0	0.0	0.01046591828793315	0.0
32-33	0.0	0.0	0.0	0.019456378170612566	0.0
34-35	0.0	0.0	0.0	0.03391587054209916	0.0
36-37	0.0	0.0	0.0	0.0562051288507989	0.0
38-39	0.0	0.0	0.0	0.08939310657963953	0.0
40-41	0.0	0.0	0.0	0.12374177825394646	0.0
42-43	0.0	0.0	0.0	0.15683139208910352	0.0
44-45	0.0	0.0	0.0	0.1966294234734809	0.0
46-47	0.0	0.0	0.0	0.2465982814647407	0.0
48-49	0.0	0.0	0.0	0.3026066825281724	0.0
50-51	0.0	0.0	0.0	0.3649497183448268	0.0
52-53	0.0	0.0	0.0	0.42369263565266213	0.0
54-55	0.0	0.0	0.0	0.48961611719939896	0.0
56-57	0.0	0.0	0.0	0.5688187243934194	0.0
58-59	0.0	0.0	0.0	0.6610447111111462	0.0
60-61	0.0	0.0	0.0	0.7626742860650233	0.0
62-63	0.0	0.0	0.0	0.8649530627172121	0.0
64-65	0.0	0.0	0.0	0.9720516701598965	0.0
66-67	0.0	0.0	0.0	1.100298514744551	0.0
68-69	0.0	0.0	0.0	1.2476476274825572	0.0
70-71	0.0	0.0	0.0	1.4170695979631591	0.0
72-73	0.0	0.0	0.0	1.601501898619876	0.0
74-75	0.0	0.0	0.0	1.7864063459662738	0.0
76-77	0.0	0.0	0.0	1.9825046044138634	0.0
78-79	0.0	0.0	0.0	2.2108858927684047	0.0
80-81	0.0	0.0	0.0	2.4686189669981267	0.0
82-83	0.0	0.0	0.0	2.7461035110795127	0.0
84-85	0.0	0.0	0.0	3.0281127942703425	0.0
86-87	0.0	0.0	0.0	3.3270209943960123	0.0
88	0.0	0.0	0.0	3.5668321671965857	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10710	0.0	65.41863	1
GTATCAA	21990	0.0	47.15069	1
GTGGTAT	3330	0.0	42.36251	1
TGGTATC	3435	0.0	40.245445	2
TATCAAC	28755	0.0	35.94268	2
ATCAACG	29155	0.0	35.446766	3
TCAACGC	29530	0.0	35.011864	4
TATAACG	220	0.0	34.197426	2
CAACGCA	30245	0.0	34.168625	5
ATAACGC	290	0.0	34.047348	3
AACGCAG	31480	0.0	32.90416	6
CGCAGAG	35065	0.0	29.352442	8
ACGCAGA	35355	0.0	29.244606	7
TACCTGG	2105	0.0	28.145863	2
CATGGGT	9325	0.0	27.983212	4
GCAGAGT	37160	0.0	27.394077	9
GTACATG	18525	0.0	26.043169	1
TACATGG	18605	0.0	25.753761	2
ACATGGG	18585	0.0	25.222874	3
TCTGTCG	700	0.0	24.84149	8
>>END_MODULE