FastQCFastQC Report
Wed 25 May 2016
SRR2431412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3542954
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254730.7189763118572807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50750.048.4960671
GTATCAA90650.038.9863851
TCAACGC103900.033.1502844
ATCAACG104300.033.0696643
TATCAAC110100.031.9263742
CAACGCA112800.030.367425
AACGCAG121350.028.0386126
GTACATG196650.027.6633471
TACATGG195250.027.2094152
ACATGGG199050.025.625793
CATGGGT85250.025.5551434
CGCAGAG132750.025.0993968
ACGCAGA137800.024.4182997
GTGGTAT24150.023.9678361
TACCTGG26100.023.7985082
GTACCTG29050.022.1929651
TGGTATC24950.021.8778042
GCAGAGT148200.021.4360089
ATGGGTG47000.020.8233725
CATGGGG87250.020.3314174
Produced by FastQC (version 0.11.5)