##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431412_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3542954 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.499040913316968 31.0 31.0 34.0 26.0 34.0 2 30.618446358603585 33.0 31.0 34.0 26.0 34.0 3 30.70742747436179 33.0 31.0 34.0 26.0 34.0 4 33.93885441357692 37.0 35.0 37.0 28.0 37.0 5 34.06811858127427 37.0 35.0 37.0 30.0 37.0 6 34.21304086928591 37.0 35.0 37.0 30.0 37.0 7 34.172230009195715 37.0 35.0 37.0 30.0 37.0 8 34.13165002989031 37.0 35.0 37.0 30.0 37.0 9 35.57218270403737 39.0 37.0 39.0 29.0 39.0 10-11 35.59138560647415 39.0 37.0 39.0 30.0 39.0 12-13 35.51707233568373 39.0 37.0 39.0 29.5 39.0 14-15 36.85768641083119 41.0 38.0 41.0 29.5 41.0 16-17 36.883972809130455 40.5 38.0 41.0 30.0 41.0 18-19 36.904219332229545 40.5 38.0 41.0 30.0 41.0 20-21 36.996699505553835 41.0 38.0 41.0 30.0 41.0 22-23 36.98229231878258 40.0 38.0 41.0 30.0 41.0 24-25 37.03457242177008 40.5 38.0 41.0 30.0 41.0 26-27 37.08808130164829 40.0 38.0 41.0 30.0 41.0 28-29 37.063610902089046 40.0 38.0 41.0 30.0 41.0 30-31 37.000478132089775 40.0 38.0 41.0 30.0 41.0 32-33 36.952871530367034 40.0 38.0 41.0 30.0 41.0 34-35 36.884968306108405 40.0 38.0 41.0 30.0 41.0 36-37 36.74901339390802 40.0 37.0 41.0 30.0 41.0 38-39 36.62398171130644 40.0 37.0 41.0 29.0 41.0 40-41 36.56008644199163 40.0 37.0 41.0 29.0 41.0 42-43 36.39525068629172 40.0 37.0 41.0 28.5 41.0 44-45 36.23950325067726 40.0 36.0 41.0 28.0 41.0 46-47 36.10442303230581 40.0 36.0 41.0 28.0 41.0 48-49 35.9712954218429 40.0 36.0 41.0 28.0 41.0 50-51 35.831621861305564 40.0 35.0 41.0 28.0 41.0 52-53 35.67174439747171 40.0 35.0 41.0 27.0 41.0 54-55 35.51249254153455 39.5 35.0 41.0 27.0 41.0 56-57 35.32423395844259 39.0 35.0 41.0 27.0 41.0 58-59 35.11450938962233 39.0 34.5 41.0 26.5 41.0 60-61 34.88098843507423 39.0 34.0 41.0 26.0 41.0 62-63 34.786311225039896 38.5 34.0 41.0 26.0 41.0 64-65 34.549659973005575 38.0 34.0 40.0 26.0 41.0 66-67 34.29933905435972 37.5 34.0 40.0 26.0 41.0 68-69 34.013748414458675 37.0 34.0 40.0 25.0 41.0 70-71 33.69986076590325 36.5 33.5 40.0 25.0 41.0 72-73 33.36922198256032 36.0 33.0 39.0 24.0 41.0 74-75 33.08584249188671 36.0 33.0 39.0 24.0 41.0 76-77 32.82278319165307 35.0 33.0 39.0 23.5 40.5 78-79 32.47118223945329 35.0 33.0 37.5 22.5 39.5 80-81 32.141808078795265 35.0 33.0 37.0 22.0 39.0 82-83 31.805758697403355 35.0 32.5 37.0 20.5 39.0 84-85 31.49666238963306 35.0 32.0 36.0 20.0 38.0 86-87 31.20612220762674 35.0 32.0 36.0 18.5 37.0 88-89 30.93523582298839 35.0 32.0 35.5 17.5 37.0 90-91 30.699448257019426 35.0 32.0 35.0 13.5 36.5 92-93 30.466637585472462 35.0 31.5 35.0 7.0 36.0 94-95 30.25156677168261 35.0 31.0 35.0 2.0 36.0 96-97 30.049888878037933 35.0 31.0 35.0 2.0 36.0 98-99 29.839034743324355 35.0 31.0 35.0 2.0 35.5 100 29.67258903163857 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3040950063379775 1101 2 0.29458813860853184 1101 3 0.24735040791369656 1101 4 0.3319539356251511 1101 5 0.2896169770083574 1101 6 0.3804009800347572 1101 7 0.31478216343923293 1101 8 0.34986480976444057 1101 9 0.38220001520219427 1101 10-11 0.31537973581256296 1101 12-13 0.2956369427354346 1101 14-15 0.3314013062631176 1101 16-17 0.2834373867986386 1101 18-19 0.23083828708587362 1101 20-21 0.2340948008824597 1101 22-23 0.30305889909325856 1101 24-25 0.3659167327607449 1101 26-27 0.35504471898571666 1101 28-29 0.3668733261250239 1101 30-31 0.3634645452342866 1101 32-33 0.3356678567222744 1101 34-35 0.37727933148280357 1101 36-37 0.37224302924308716 1101 38-39 0.41078885399938514 1101 40-41 0.2736381871829252 1101 42-43 0.31554113097824654 1101 44-45 0.3069967033007259 1101 46-47 0.3133004703926545 1101 48-49 0.31402898261576695 1101 50-51 0.2829619543360238 1101 52-53 0.2418655587929308 1101 54-55 0.26815430867818435 1101 56-57 0.1849329399215378 1101 58-59 0.20321045496417156 1101 60-61 0.2605439665861269 1101 62-63 0.24346828368714313 1101 64-65 0.31357439716064306 1101 66-67 0.3734944629764101 1101 68-69 0.3292335868208198 1101 70-71 0.3227789716356 1101 72-73 0.26025586586823835 1101 74-75 0.29357845128151183 1101 76-77 0.2530994141165053 1101 78-79 0.24360035967412585 1101 80-81 0.22654432432481997 1101 82-83 0.16244506463569408 1101 84-85 0.18920967817154022 1101 86-87 0.12772009825670239 1101 88-89 0.15177725053406022 1101 90-91 0.1690572949536282 1101 92-93 0.1512841663109903 1101 94-95 0.15378027399219718 1101 96-97 0.13743852884566365 1101 98-99 0.06519078657460398 1101 100 0.11781341765043152 1102 1 -0.10984537726090338 1102 2 -0.05486884779694634 1102 3 -0.034194102515428426 1102 4 0.07544212658665828 1102 5 0.08466923224727907 1102 6 0.027236009131080152 1102 7 0.015231031130745976 1102 8 0.09327490103602543 1102 9 0.09326100177331398 1102 10-11 0.03558736832931686 1102 12-13 0.006839589660280865 1102 14-15 0.0637671422616819 1102 16-17 -0.003240922253247902 1102 18-19 0.01844473407064129 1102 20-21 -0.06381075725781216 1102 22-23 -0.028879322072889124 1102 24-25 -0.040757791577128444 1102 26-27 -0.03834113550100682 1102 28-29 -0.041996971756020685 1102 30-31 -0.07886758762713697 1102 32-33 0.024456197123662093 1102 34-35 -0.05981251009875166 1102 36-37 -0.033138282977859035 1102 38-39 -0.05417224535194265 1102 40-41 -0.013122712671652437 1102 42-43 0.00155228903374649 1102 44-45 -0.10362855398675208 1102 46-47 -0.11631549531534091 1102 48-49 -0.16428746819329376 1102 50-51 -0.22301069280805308 1102 52-53 -0.1897325100590237 1102 54-55 -0.25443857666842007 1102 56-57 -0.230552690953445 1102 58-59 -0.2643439194710169 1102 60-61 -0.3242277661369428 1102 62-63 -0.2314355303789668 1102 64-65 -0.1815144568413487 1102 66-67 -0.20901953407133078 1102 68-69 -0.22361805665241263 1102 70-71 -0.2255386270714439 1102 72-73 -0.2110600819362034 1102 74-75 -0.1850328144389053 1102 76-77 -0.17886106470005814 1102 78-79 -0.23050906027506812 1102 80-81 -0.25828279371440743 1102 82-83 -0.28421657705937164 1102 84-85 -0.3449148623647851 1102 86-87 -0.337626703949212 1102 88-89 -0.3586376399863127 1102 90-91 -0.3134718957569831 1102 92-93 -0.3402905822475901 1102 94-95 -0.3382880402480737 1102 96-97 -0.3138740026682463 1102 98-99 -0.2814020116900906 1102 100 -0.34756192976378486 1103 1 -0.4657108536039338 1103 2 -0.4599628214811595 1103 3 -0.44101493192365737 1103 4 -0.25793541443693613 1103 5 -0.2595117113982113 1103 6 -0.23390287436102142 1103 7 -0.1983842408617562 1103 8 -0.22405310502184506 1103 9 -0.08527793674301165 1103 10-11 -0.12697278666323086 1103 12-13 -0.21528126107480006 1103 14-15 -0.24086449170506796 1103 16-17 -0.29313440124001033 1103 18-19 -0.2686716599168619 1103 20-21 -0.3555395634787004 1103 22-23 -0.3363816618270903 1103 24-25 -0.3051177124488049 1103 26-27 -0.2865620343248736 1103 28-29 -0.3329344029087622 1103 30-31 -0.29144487836247635 1103 32-33 -0.27348878447292435 1103 34-35 -0.3072191645082114 1103 36-37 -0.3692536005176734 1103 38-39 -0.3464804976531539 1103 40-41 -0.266216657726126 1103 42-43 -0.30499083952955885 1103 44-45 -0.4245031841922966 1103 46-47 -0.47572847613233904 1103 48-49 -0.48683686177095353 1103 50-51 -0.5466141240290838 1103 52-53 -0.6296111804264299 1103 54-55 -0.5792590398609079 1103 56-57 -0.5564148289460462 1103 58-59 -0.4937563598442196 1103 60-61 -0.49447462417582244 1103 62-63 -0.38683411610031015 1103 64-65 -0.44831048937147244 1103 66-67 -0.4358730572673153 1103 68-69 -0.4245904268077325 1103 70-71 -0.5054376850304507 1103 72-73 -0.4908200292023608 1103 74-75 -0.5451177712853124 1103 76-77 -0.4002332361925198 1103 78-79 -0.416500726417361 1103 80-81 -0.3267282017716724 1103 82-83 -0.39492436476263393 1103 84-85 -0.4824507471440036 1103 86-87 -0.5082913342297708 1103 88-89 -0.5927418559225472 1103 90-91 -0.4729998816362446 1103 92-93 -0.5286049510559288 1103 94-95 -0.6342461253868024 1103 96-97 -0.6166298335286271 1103 98-99 -0.6218366502181638 1103 100 -0.6493686349106618 1104 1 -0.3798808998036911 1104 2 -0.31538394691016336 1104 3 -0.29506136675194483 1104 4 -0.21431920647280833 1104 5 -0.1988826354665676 1104 6 -0.09786253037499648 1104 7 -0.11559227209911427 1104 8 -0.13086479672860207 1104 9 -0.09174058360250825 1104 10-11 -0.11164263802599095 1104 12-13 -0.1216067044489435 1104 14-15 -0.15272938732225327 1104 16-17 -0.13788285664799105 1104 18-19 -0.1636379036633855 1104 20-21 -0.21831985909447127 1104 22-23 -0.14427113539768044 1104 24-25 -0.1697553048303888 1104 26-27 -0.16431757591119833 1104 28-29 -0.13655992268116535 1104 30-31 -0.17143236675828888 1104 32-33 -0.2277328410341184 1104 34-35 -0.13960487035136282 1104 36-37 -0.2987521639671442 1104 38-39 -0.27803386957752707 1104 40-41 -0.17833032097230017 1104 42-43 -0.29461436119368756 1104 44-45 -0.3158136781760561 1104 46-47 -0.4434341924876932 1104 48-49 -0.45687032152923734 1104 50-51 -0.5314702950236878 1104 52-53 -0.5033206343285599 1104 54-55 -0.4474280171603766 1104 56-57 -0.4683883446214665 1104 58-59 -0.545801491914716 1104 60-61 -0.5898845028950603 1104 62-63 -0.4676828936466251 1104 64-65 -0.4316621606388438 1104 66-67 -0.4453264927437175 1104 68-69 -0.42073557188759025 1104 70-71 -0.44659201918513247 1104 72-73 -0.44634743763210594 1104 74-75 -0.3775971870634294 1104 76-77 -0.3131807850725892 1104 78-79 -0.33258992932179154 1104 80-81 -0.3587040803032622 1104 82-83 -0.46385191070696763 1104 84-85 -0.3856577363420577 1104 86-87 -0.3456365909572412 1104 88-89 -0.3228616441563048 1104 90-91 -0.368361717889627 1104 92-93 -0.421029834480958 1104 94-95 -0.3592359017087752 1104 96-97 -0.411106494082901 1104 98-99 -0.4459643482533373 1104 100 -0.40622766151635403 1105 1 -0.25406523324591035 1105 2 -0.18658575847644343 1105 3 -0.21807292440538006 1105 4 -0.05380915890341953 1105 5 -0.133206656718464 1105 6 -0.04506404393379171 1105 7 -0.1280154498083661 1105 8 -0.07631248577954608 1105 9 -0.21356763302232196 1105 10-11 -0.15922096767143756 1105 12-13 -0.15548732474272242 1105 14-15 -0.2004473844484238 1105 16-17 -0.22007795961999932 1105 18-19 -0.10075749565665149 1105 20-21 -0.11219132156847422 1105 22-23 -0.13872565878127574 1105 24-25 -0.23485224704995744 1105 26-27 -0.16510370430265908 1105 28-29 -0.12533039370804744 1105 30-31 -0.18981844238480505 1105 32-33 -0.12833121266188385 1105 34-35 -0.1461407437555522 1105 36-37 -0.1904367386275183 1105 38-39 -0.2363725659287894 1105 40-41 -0.3124757974908263 1105 42-43 -0.3029409900487394 1105 44-45 -0.331217589565739 1105 46-47 -0.3146065458858729 1105 48-49 -0.4049279101585128 1105 50-51 -0.299209373280803 1105 52-53 -0.33302435743819814 1105 54-55 -0.3828205887553011 1105 56-57 -0.43591885528380203 1105 58-59 -0.4156992195549236 1105 60-61 -0.5085837047311799 1105 62-63 -0.37569422034049893 1105 64-65 -0.31113287057994654 1105 66-67 -0.34841472897345227 1105 68-69 -0.31165090248673266 1105 70-71 -0.25621537965925256 1105 72-73 -0.24509685651562307 1105 74-75 -0.3410923428041528 1105 76-77 -0.36691948588870105 1105 78-79 -0.3683280894628709 1105 80-81 -0.31697397818525275 1105 82-83 -0.3503950091739334 1105 84-85 -0.3534307451383576 1105 86-87 -0.3101883631888711 1105 88-89 -0.3952833551687718 1105 90-91 -0.4131923063177574 1105 92-93 -0.43167718346606065 1105 94-95 -0.43673883613760367 1105 96-97 -0.38386614016475207 1105 98-99 -0.4213531876018912 1105 100 -0.4659896447885572 1106 1 -0.30777457628383686 1106 2 -0.25497581017135573 1106 3 -0.2732816176130619 1106 4 -0.24782151361520022 1106 5 -0.20508104971236207 1106 6 -0.138180656065515 1106 7 -0.09063614029954437 1106 8 -0.17003747496347188 1106 9 -0.1782096808924294 1106 10-11 -0.1939726893680387 1106 12-13 -0.1277401287681741 1106 14-15 -0.2831397987084898 1106 16-17 -0.23142367032186684 1106 18-19 -0.2195211167653568 1106 20-21 -0.32493177174801247 1106 22-23 -0.2972249275599026 1106 24-25 -0.27960046633036484 1106 26-27 -0.36461246770297606 1106 28-29 -0.3275084506593231 1106 30-31 -0.2756640233515171 1106 32-33 -0.3999520845513729 1106 34-35 -0.3196025957039268 1106 36-37 -0.3273477316541502 1106 38-39 -0.3425729285233885 1106 40-41 -0.3737428445311508 1106 42-43 -0.36092439563834944 1106 44-45 -0.31468041633164745 1106 46-47 -0.3608604289210433 1106 48-49 -0.3756775939020187 1106 50-51 -0.38852901342233537 1106 52-53 -0.4318723338151429 1106 54-55 -0.3766499252646227 1106 56-57 -0.37022106055049164 1106 58-59 -0.4133357741009789 1106 60-61 -0.4552538081527473 1106 62-63 -0.3898249616260827 1106 64-65 -0.3177876236920767 1106 66-67 -0.2527329072494666 1106 68-69 -0.2742604630294636 1106 70-71 -0.3366696821473738 1106 72-73 -0.29270312750018235 1106 74-75 -0.3041870031419336 1106 76-77 -0.25244363683057713 1106 78-79 -0.22985304103947612 1106 80-81 -0.25101983386146287 1106 82-83 -0.25293522779282185 1106 84-85 -0.261872235594673 1106 86-87 -0.23099436050296163 1106 88-89 -0.26585329295073734 1106 90-91 -0.2528258759451312 1106 92-93 -0.2688112408629699 1106 94-95 -0.3240601039574287 1106 96-97 -0.3123421150616501 1106 98-99 -0.3602791919525643 1106 100 -0.3699389112726408 1107 1 -0.608828737187963 1107 2 -0.6357760001606003 1107 3 -0.5899443028874956 1107 4 -0.44625857269347335 1107 5 -0.4374731679527244 1107 6 -0.4200233421781405 1107 7 -0.5079924958588222 1107 8 -0.4967850267564984 1107 9 -0.5016143998899594 1107 10-11 -0.46662845260790675 1107 12-13 -0.5328305133566928 1107 14-15 -0.627789436192252 1107 16-17 -0.6101562953864743 1107 18-19 -0.6427282329801045 1107 20-21 -0.5877689328058011 1107 22-23 -0.6282818824234866 1107 24-25 -0.6543657388112578 1107 26-27 -0.6255578388106429 1107 28-29 -0.6704146574402685 1107 30-31 -0.6710022670685944 1107 32-33 -0.6666654452655152 1107 34-35 -0.703973815127533 1107 36-37 -0.6562628371959605 1107 38-39 -0.7802326731554956 1107 40-41 -0.6935860684809754 1107 42-43 -0.7597034004299914 1107 44-45 -0.7641545582368465 1107 46-47 -0.7030179593402792 1107 48-49 -0.7475164732448789 1107 50-51 -0.8765943012700106 1107 52-53 -0.817864049725344 1107 54-55 -0.8408111437103472 1107 56-57 -0.8568808820164833 1107 58-59 -0.9802754106068505 1107 60-61 -0.9887478290795002 1107 62-63 -1.0311783555883451 1107 64-65 -0.8527523150293987 1107 66-67 -0.8932807677750105 1107 68-69 -0.9704371041314062 1107 70-71 -0.9134544634501935 1107 72-73 -0.8373543016616409 1107 74-75 -0.8733413065379949 1107 76-77 -0.9158840137357025 1107 78-79 -0.8347223885102437 1107 80-81 -0.8166739346954586 1107 82-83 -0.8279673583266067 1107 84-85 -0.8590813145659375 1107 86-87 -0.891759625998958 1107 88-89 -0.9425267698935897 1107 90-91 -0.8649981794250046 1107 92-93 -0.7913588778717582 1107 94-95 -0.8725973264823281 1107 96-97 -0.9036627847737329 1107 98-99 -0.919544066294236 1107 100 -0.8995514589445754 1108 1 -0.40824321084933857 1108 2 -0.4068923939957756 1108 3 -0.3730827268388275 1108 4 -0.37206018365439775 1108 5 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16-17 0.22756046948691733 2215 18-19 0.3617257240674121 2215 20-21 0.2529368332142212 2215 22-23 0.23024189081890256 2215 24-25 0.2892593293774297 2215 26-27 0.2842504213748285 2215 28-29 0.28965627381802506 2215 30-31 0.23166646141346092 2215 32-33 0.2342003518921345 2215 34-35 0.27037832851426913 2215 36-37 0.21210968114922935 2215 38-39 0.251480977955886 2215 40-41 0.2636025590743216 2215 42-43 0.3010771139117949 2215 44-45 0.3563143995695768 2215 46-47 0.37234402300805414 2215 48-49 0.333323040274891 2215 50-51 0.3572849773350484 2215 52-53 0.3672734021293067 2215 54-55 0.3668059116506015 2215 56-57 0.34313710627084504 2215 58-59 0.3478522746747572 2215 60-61 0.4109900709044183 2215 62-63 0.32899239037706707 2215 64-65 0.29111031276332966 2215 66-67 0.2824997390528665 2215 68-69 0.3188848110726781 2215 70-71 0.2612454838021989 2215 72-73 0.24060116492928785 2215 74-75 0.34224156957424867 2215 76-77 0.3817834298686904 2215 78-79 0.38614026783532296 2215 80-81 0.3370380543402547 2215 82-83 0.3515257687049633 2215 84-85 0.33500446116204685 2215 86-87 0.36802845183907706 2215 88-89 0.4191916600610419 2215 90-91 0.45111535867725294 2215 92-93 0.4276903577387401 2215 94-95 0.4360283202769999 2215 96-97 0.43965663280850364 2215 98-99 0.4503942595777879 2215 100 0.4310999815460512 2216 1 0.6610896796652774 2216 2 0.5002707691916548 2216 3 0.5564026116842555 2216 4 0.5032206262213421 2216 5 0.5189672643856085 2216 6 0.45271056814470967 2216 7 0.43034981835070596 2216 8 0.3743027136371069 2216 9 0.361882832564433 2216 10-11 0.3315413928851285 2216 12-13 0.3866222588999477 2216 14-15 0.5019760440415979 2216 16-17 0.5455351273906999 2216 18-19 0.49677671958801284 2216 20-21 0.4705470295238712 2216 22-23 0.46227847639280384 2216 24-25 0.47974537878064183 2216 26-27 0.46554345141449005 2216 28-29 0.4392335105656784 2216 30-31 0.4132433767203878 2216 32-33 0.4470700309153486 2216 34-35 0.4337790215880233 2216 36-37 0.497315873715543 2216 38-39 0.5748723829058093 2216 40-41 0.5364139464746884 2216 42-43 0.600029760614099 2216 44-45 0.6695117180869943 2216 46-47 0.7145020775104811 2216 48-49 0.7189527487465242 2216 50-51 0.7932531210465328 2216 52-53 0.7749963705587177 2216 54-55 0.8453039281591046 2216 56-57 0.8512263889786453 2216 58-59 0.918434797979792 2216 60-61 0.883770973928975 2216 62-63 0.8089164334096708 2216 64-65 0.8287402602312355 2216 66-67 0.7848855332256619 2216 68-69 0.7622167186110715 2216 70-71 0.7126043003445375 2216 72-73 0.687593899396326 2216 74-75 0.6863350229831369 2216 76-77 0.7060054549344272 2216 78-79 0.7031166376965672 2216 80-81 0.6790287496966556 2216 82-83 0.6759288164632302 2216 84-85 0.6883605639850963 2216 86-87 0.6842297435151146 2216 88-89 0.6761119954864405 2216 90-91 0.742395422242847 2216 92-93 0.7289470103226883 2216 94-95 0.669635210162788 2216 96-97 0.7059270411872056 2216 98-99 0.6726195696071322 2216 100 0.6942422097246563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84295.0 3 16836.0 4 12739.0 5 11128.0 6 7406.0 7 6023.0 8 6744.0 9 7092.0 10 7355.0 11 7714.0 12 9049.0 13 10341.0 14 10969.0 15 11055.0 16 11038.0 17 10735.0 18 10792.0 19 10296.0 20 9923.0 21 10380.0 22 12471.0 23 18991.0 24 30850.0 25 40774.0 26 45508.0 27 35214.0 28 31446.0 29 38705.0 30 46303.0 31 52144.0 32 66388.0 33 88536.0 34 126382.0 35 202123.0 36 339036.0 37 615866.0 38 966358.0 39 511836.0 40 2113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.64699854634518 22.284944055072057 14.31811826207239 21.749939136510374 2 14.447677451514554 22.988857388197125 40.69409969455293 21.869365465735388 3 17.116368639017278 29.654116372880736 30.776898485848932 22.452616502253058 4 11.353883912414043 20.070745114006513 39.63801800579081 28.937352967788637 5 11.734319471910236 41.09883528075341 34.68718124481614 12.479664002520208 6 26.657264349361714 41.28118201421251 19.204143648125477 12.8574099883003 7 24.095003484259536 36.25420773418052 23.30298765015718 16.34780113140277 8 23.5351269307646 37.976055680470516 21.201390373636787 17.287427015128095 9 24.709532201398538 19.16124422453429 21.730634538697032 34.39858903537014 10-11 21.7964017732685 29.997330776150978 29.402432806879535 18.803834643700984 12-13 22.598962771689163 28.347089323598457 28.90202708905244 20.15192081565994 14-15 20.59868297202788 29.80442011749 26.51107345448198 23.08582345600014 16-17 19.665274448177612 32.79948325609665 27.183960377695282 20.351281918030452 18-19 19.845921608441593 31.624666834535127 28.962322945495522 19.567088611527762 20-21 21.703882286498533 30.480692086108835 28.216303883162098 19.599121744230533 22-23 21.255360500451776 30.551131329330676 28.28278135089554 19.910726819322008 24-25 20.788806737604176 30.55432769559668 28.311472404784993 20.34539316201415 26-27 20.467950644455694 30.61487953740193 28.39993028941755 20.517239528724826 28-29 20.41911020990817 30.578968284566976 28.554467465039078 20.44745404048578 30-31 20.657399861873575 30.881466908270216 28.185586550045404 20.275546679810805 32-33 20.388925945640356 30.72455372192504 28.58617281181844 20.300347520616164 34-35 20.64415137033245 30.535678045488833 28.413057020839812 20.407113563338903 36-37 20.773771329079157 30.501457143676358 28.202937112382337 20.521834414862145 38-39 20.607438138506414 30.560401949467348 28.443144937645943 20.3890149743803 40-41 20.48411235781454 30.342629164725338 28.613757337095336 20.559501140364787 42-43 20.468061655343377 30.413824545307662 28.558743640182634 20.559370159166328 44-45 20.64243599779538 30.462006887883884 28.423157098387808 20.47240001593293 46-47 20.66274482221339 30.586827585447146 28.34309416127074 20.407333431068725 48-49 20.599450106936207 30.352664902240452 28.394959718482777 20.65292527234057 50-51 20.526729618597848 30.33825093213951 28.368565539728515 20.76645390953413 52-53 20.583921708758048 30.37695103125181 28.363683100690235 20.67544415929991 54-55 20.644580110235047 30.590521887690418 28.251685276457657 20.51321272561688 56-57 20.658088705716132 30.3760006530496 28.08501677676522 20.880893864469048 58-59 20.611068267783043 30.20172145433796 28.39951921311401 20.78769106476499 60-61 20.75496108081113 30.44747180632718 28.08689786551779 20.7106692473439 62-63 20.601756263998848 30.38473705468483 28.23780458526693 20.775702096049393 64-65 20.696362539662186 30.240467906095088 28.302604658243908 20.76056489599882 66-67 20.55123739388037 30.273202265090625 28.207089480570254 20.96847086045876 68-69 20.849912686974506 30.51446543456325 27.932033535545546 20.703588342916703 70-71 20.822780609254764 30.172176396073496 28.065162100292184 20.939880894379552 72-73 20.642695641011628 30.318660460460716 28.316894965175177 20.721748933352483 74-75 20.677497187863434 30.406677668069733 28.14999191488671 20.765833229180128 76-77 20.753476274784735 30.358369554532917 28.009871524431375 20.87828264625098 78-79 20.76802827700289 30.32099307324716 28.257527708353013 20.653450941396933 80-81 20.68784343656757 30.32927821719894 28.190815779163348 20.792062567070143 82-83 20.60183710667351 30.32165256269107 28.408996695693812 20.66751363494161 84-85 20.609282800065877 30.18133174539997 28.186857685709533 21.022527768824624 86-87 20.770253539459667 30.233811532193894 28.14038860966414 20.855546318682297 88-89 20.696225237211483 30.10118386820674 28.240426090862098 20.96216480371968 90-91 20.600947051470058 30.110556190416464 28.17538149208839 21.11311526602508 92-93 20.693438517034096 30.181494136728926 28.130454339074802 20.99461300716218 94-95 20.551205544113277 30.082720515546495 28.32363501404896 21.04243892629127 96-97 20.56948912989138 30.239113862520124 28.150701781590058 21.040695225998444 98-99 20.522864259577407 30.20821975541177 28.116474594043083 21.152441390967738 100 20.589420122649198 30.324205644755768 27.95116857992933 21.135205652665704 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22329.0 1 16339.0 2 8998.0 3 6484.5 4 4431.0 5 3139.0 6 2675.0 7 2814.0 8 3244.0 9 3239.0 10 2460.0 11 1782.0 12 1649.0 13 1686.5 14 1764.0 15 1884.0 16 2095.0 17 2534.5 18 3367.5 19 4435.5 20 5934.0 21 8328.0 22 12450.0 23 18833.5 24 25269.5 25 32004.0 26 41281.0 27 52438.5 28 66884.0 29 79726.0 30 86612.5 31 93969.0 32 101765.5 33 108719.5 34 115976.5 35 121397.0 36 122877.5 37 123117.5 38 122616.0 39 120441.0 40 119297.0 41 116067.0 42 109914.0 43 105244.5 44 102438.0 45 99476.5 46 97656.0 47 95295.5 48 92808.5 49 92090.0 50 91724.0 51 89072.5 52 84028.0 53 79475.5 54 76869.0 55 75264.0 56 68754.0 57 60450.5 58 55284.0 59 50922.5 60 44847.5 61 37636.0 62 31692.5 63 25832.0 64 20458.5 65 16609.0 66 13274.0 67 10219.0 68 7853.5 69 6034.5 70 4537.0 71 3396.5 72 2603.5 73 1996.0 74 1602.0 75 1281.5 76 886.5 77 672.5 78 529.5 79 406.0 80 299.5 81 213.5 82 178.0 83 138.0 84 106.0 85 92.5 86 85.0 87 85.0 88 71.5 89 51.0 90 39.0 91 35.5 92 31.5 93 27.0 94 29.0 95 27.0 96 20.5 97 19.0 98 16.0 99 15.0 100 29.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1792571961137514 2 0.17417668984694692 3 0.1876682564887944 4 0.17821286982557494 5 0.19015205955256545 6 0.07598179372354255 7 0.07753417063839949 8 0.06556675587659337 9 0.05966772360013706 10-11 0.07335686548569358 12-13 0.0721714140234392 14-15 0.07006864892967846 16-17 0.0655385308417778 18-19 0.07009687396449404 20-21 0.07133877549637957 22-23 0.06980051109893043 24-25 0.06963116089003696 26-27 0.07344154059014033 28-29 0.07059081207376669 30-31 0.07674386966356322 32-33 0.07306050262012999 34-35 0.07311695268976114 36-37 0.08412471626783752 38-39 0.07924178524474211 40-41 0.07449997939572459 42-43 0.07784464602137087 44-45 0.08792098345053309 46-47 0.07855027189176038 48-49 0.08447752920303228 50-51 0.08031433656773415 52-53 0.08013087384143289 54-55 0.08134455033850284 56-57 0.07454231694794795 58-59 0.08253000180075722 60-61 0.07897364741399408 62-63 0.07011098648190182 64-65 0.07297582751568324 66-67 0.07366734086866496 68-69 0.0714516756356419 70-71 0.06771185852257748 72-73 0.06504459273250512 74-75 0.07046379941709659 76-77 0.07536084295759979 78-79 0.07198795129713792 80-81 0.07950992307549011 82-83 0.08118931264701715 84-85 0.08250177676594164 86-87 0.07888897230954735 88-89 0.0808364997118224 90-91 0.07927001027955767 92-93 0.07004042389486287 94-95 0.06994163627300834 96-97 0.0695747108204058 98-99 0.07903009748362524 100 0.08755405799793055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3542954.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.3208733947976 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3489779925503 47.58134991295916 2 14.632254973740807 18.23789819397797 3 4.434970351378607 8.291736773338416 4 1.798867350834701 4.484279377016175 5 0.8513093150532778 2.6527170021623596 6 0.4906021738302098 1.8344853577490998 7 0.3337847823067103 1.4561231411472602 8 0.21908407948140404 1.0922808944141085 9 0.15462715427878243 0.8672849374685253 >10 0.6728740800716979 7.280602759205978 >50 0.038531351864886806 1.6313628748814535 >100 0.02194241744061952 2.7079191889274097 >500 0.0014493181216641384 0.5920939886299954 >1k 6.793678558868867E-4 0.5708681281899063 >5k 0.0 0.0 >10k+ 4.529119039245892E-5 0.7189974699323332 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25473 0.7189763118572807 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.8225034815580443E-5 0.0 3 0.0 0.0 0.0 2.8225034815580443E-5 0.0 4 0.0 0.0 0.0 2.8225034815580443E-5 0.0 5 0.0 2.8225034815580443E-5 0.0 2.8225034815580443E-5 0.0 6 0.0 2.8225034815580443E-5 0.0 2.8225034815580443E-5 0.0 7 0.0 2.8225034815580443E-5 0.0 2.8225034815580443E-5 0.0 8 0.0 2.8225034815580443E-5 0.0 2.8225034815580443E-5 2.8225034815580443E-5 9 0.0 2.8225034815580443E-5 0.0 5.645006963116089E-5 2.8225034815580443E-5 10-11 0.0 2.8225034815580443E-5 0.0 5.645006963116089E-5 2.8225034815580443E-5 12-13 0.0 2.8225034815580443E-5 0.0 5.645006963116089E-5 2.8225034815580443E-5 14-15 5.645006963116089E-5 2.8225034815580443E-5 0.0 5.645006963116089E-5 2.8225034815580443E-5 16-17 5.645006963116089E-5 2.8225034815580443E-5 0.0 8.467510444674133E-5 2.8225034815580443E-5 18-19 5.645006963116089E-5 2.8225034815580443E-5 0.0 1.1290013926232177E-4 2.8225034815580443E-5 20-21 5.645006963116089E-5 2.8225034815580443E-5 0.0 1.1290013926232177E-4 2.8225034815580443E-5 22-23 5.645006963116089E-5 2.8225034815580443E-5 0.0 3.104753829713849E-4 2.8225034815580443E-5 24-25 5.645006963116089E-5 2.8225034815580443E-5 0.0 7.197383877973014E-4 2.8225034815580443E-5 26-27 5.645006963116089E-5 2.8225034815580443E-5 0.0 0.0011713389448465884 2.8225034815580443E-5 28-29 7.056258703895111E-5 2.8225034815580443E-5 0.0 0.0026249282378489813 2.8225034815580443E-5 30-31 8.467510444674133E-5 2.8225034815580443E-5 0.0 0.0064494204553601315 2.8225034815580443E-5 32-33 8.467510444674133E-5 2.8225034815580443E-5 0.0 0.013463341607031873 2.8225034815580443E-5 34-35 8.467510444674133E-5 2.8225034815580443E-5 0.0 0.02348322896656293 2.8225034815580443E-5 36-37 8.467510444674133E-5 2.8225034815580443E-5 0.0 0.039726736502929474 2.8225034815580443E-5 38-39 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.06487524252361165 2.8225034815580443E-5 40-41 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.09175958818545203 2.8225034815580443E-5 42-43 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.11759960755911592 2.8225034815580443E-5 44-45 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.14974792221406205 2.8225034815580443E-5 46-47 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.19152097374112112 2.8225034815580443E-5 48-49 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.23593306602343694 2.8225034815580443E-5 50-51 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.2844377883540119 2.8225034815580443E-5 52-53 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.33151714642640007 2.8225034815580443E-5 54-55 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.3858785634812081 2.8225034815580443E-5 56-57 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.45009051768665354 2.8225034815580443E-5 58-59 1.1290013926232177E-4 2.8225034815580443E-5 0.0 0.5267355997283623 2.8225034815580443E-5 60-61 1.4112517407790221E-4 2.8225034815580443E-5 0.0 0.6080801500668651 2.8225034815580443E-5 62-63 1.6935020889348267E-4 2.8225034815580443E-5 0.0 0.6924306666132273 2.8225034815580443E-5 64-65 1.6935020889348267E-4 2.8225034815580443E-5 0.0 0.7819322520134329 2.8225034815580443E-5 66-67 1.6935020889348267E-4 2.8225034815580443E-5 0.0 0.8875644448107427 2.8225034815580443E-5 68-69 1.9757524370906312E-4 2.8225034815580443E-5 0.0 1.0100893209451773 2.8225034815580443E-5 70-71 1.9757524370906312E-4 2.8225034815580443E-5 0.0 1.1488153670637553 2.8225034815580443E-5 72-73 1.9757524370906312E-4 2.8225034815580443E-5 0.0 1.2938497084636154 2.8225034815580443E-5 74-75 1.9757524370906312E-4 2.8225034815580443E-5 0.0 1.4377691609882601 2.8225034815580443E-5 76-77 1.9757524370906312E-4 2.8225034815580443E-5 0.0 1.5970994825222116 2.8225034815580443E-5 78-79 2.2580027852464355E-4 4.233755222337067E-5 0.0 1.787265654592185 2.8225034815580443E-5 80-81 2.2580027852464355E-4 5.645006963116089E-5 0.0 2.0047113228114166 2.8225034815580443E-5 82-83 2.2580027852464355E-4 5.645006963116089E-5 0.0 2.237116259482906 2.8225034815580443E-5 84-85 2.2580027852464355E-4 5.645006963116089E-5 0.0 2.466317654702827 2.8225034815580443E-5 86-87 2.2580027852464355E-4 5.645006963116089E-5 0.0 2.7119460201854158 2.8225034815580443E-5 88 2.2580027852464355E-4 5.645006963116089E-5 0.0 2.917283148468764 2.8225034815580443E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5075 0.0 48.496067 1 GTATCAA 9065 0.0 38.986385 1 TCAACGC 10390 0.0 33.150284 4 ATCAACG 10430 0.0 33.069664 3 TATCAAC 11010 0.0 31.926374 2 CAACGCA 11280 0.0 30.36742 5 AACGCAG 12135 0.0 28.038612 6 GTACATG 19665 0.0 27.663347 1 TACATGG 19525 0.0 27.209415 2 ACATGGG 19905 0.0 25.62579 3 CATGGGT 8525 0.0 25.555143 4 CGCAGAG 13275 0.0 25.099396 8 ACGCAGA 13780 0.0 24.418299 7 GTGGTAT 2415 0.0 23.967836 1 TACCTGG 2610 0.0 23.798508 2 GTACCTG 2905 0.0 22.192965 1 TGGTATC 2495 0.0 21.877804 2 GCAGAGT 14820 0.0 21.436008 9 ATGGGTG 4700 0.0 20.823372 5 CATGGGG 8725 0.0 20.331417 4 >>END_MODULE