FastQCFastQC Report
Wed 25 May 2016
SRR2431412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3542954
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312820.8829355391009874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248630.7017590406197766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172290.4862891248376354No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57550.16243507536366547No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54050.1525563131782123No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52400.14789918243364153No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48560.13706076906445866No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44440.1254320547204395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102050.066.036251
GTATCAA212050.047.370961
GTGGTAT30600.038.267241
TATCAAC276650.036.153922
ATCAACG276500.036.0354423
TCAACGC277050.035.9289444
TGGTATC32250.035.5777662
CAACGCA289400.034.4606865
AACGCAG299850.033.3377886
CGCAGAG332550.029.8900578
ACGCAGA335000.029.7416027
GCAGAGT355350.027.8399949
GTACATG179900.027.3693661
CATGGGT81950.027.0816294
TACATGG183500.026.1129952
ACATGGG183700.025.2383983
GAGTACT257800.023.85416212-13
CAGAGTA353050.023.14923910-11
AGAGTAC342800.022.72406810-11
TATAACG2704.7293724E-1122.6411342
Produced by FastQC (version 0.11.5)