##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431412_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3542954
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62409023656531	34.0	31.0	34.0	31.0	34.0
2	32.76710027846819	34.0	31.0	34.0	31.0	34.0
3	32.84760090026571	34.0	31.0	34.0	31.0	34.0
4	36.31090468575093	37.0	37.0	37.0	35.0	37.0
5	36.22709411412059	37.0	37.0	37.0	35.0	37.0
6	36.27619015093055	37.0	37.0	37.0	35.0	37.0
7	36.22730664863275	37.0	37.0	37.0	35.0	37.0
8	36.2454513945143	37.0	37.0	37.0	35.0	37.0
9	38.039464525929496	39.0	39.0	39.0	35.0	39.0
10-11	38.01511619964583	39.0	39.0	39.0	35.0	39.0
12-13	38.00016624545506	39.0	39.0	39.0	35.0	39.0
14-15	39.45021075633497	41.0	39.0	41.0	36.0	41.0
16-17	39.48186993113656	41.0	39.0	41.0	36.0	41.0
18-19	39.56107093120599	41.0	39.5	41.0	36.5	41.0
20-21	39.56191514199733	41.0	40.0	41.0	37.0	41.0
22-23	39.496990646787964	41.0	39.5	41.0	36.0	41.0
24-25	39.44266860365673	41.0	39.0	41.0	36.0	41.0
26-27	39.31786709621406	41.0	39.0	41.0	36.0	41.0
28-29	39.23021622634671	41.0	39.0	41.0	35.0	41.0
30-31	39.035336332337366	41.0	39.0	41.0	35.0	41.0
32-33	38.79444356319614	41.0	38.0	41.0	35.0	41.0
34-35	38.65173821054408	41.0	38.0	41.0	35.0	41.0
36-37	38.501617576745275	41.0	38.0	41.0	35.0	41.0
38-39	38.34733346806083	40.5	38.0	41.0	35.0	41.0
40-41	38.197621391640986	40.0	38.0	41.0	34.0	41.0
42-43	38.01369704489531	40.0	38.0	41.0	34.0	41.0
44-45	37.74664489011147	40.0	38.0	41.0	33.0	41.0
46-47	37.34969025846793	40.0	37.5	41.0	33.0	41.0
48-49	36.99249143511318	40.0	37.0	41.0	31.0	41.0
50-51	36.81965007166336	40.0	37.0	41.0	31.0	41.0
52-53	36.75851873888287	40.0	37.0	41.0	31.0	41.0
54-55	36.6175180372085	40.0	36.0	41.0	31.0	41.0
56-57	36.469575670471585	40.0	36.0	41.0	31.0	41.0
58-59	36.29731419036205	40.0	35.5	41.0	30.5	41.0
60-61	36.1464599879084	39.5	35.0	41.0	30.0	41.0
62-63	35.989933400207846	39.0	35.0	41.0	30.0	41.0
64-65	35.763353405096424	39.0	35.0	41.0	30.0	41.0
66-67	35.50552688519241	39.0	35.0	41.0	29.0	41.0
68-69	35.21861051540607	38.0	35.0	41.0	29.0	41.0
70-71	34.924353519690065	37.0	35.0	40.0	29.0	41.0
72-73	34.61216247797742	37.0	35.0	39.5	29.0	41.0
74-75	34.279685962617634	36.0	35.0	39.0	28.0	41.0
76-77	33.94786370356488	36.0	35.0	39.0	28.0	41.0
78-79	33.63050748048098	35.0	34.0	38.5	28.0	40.0
80-81	33.36176041235647	35.0	34.0	37.0	28.0	39.0
82-83	33.15443976975146	35.0	34.0	37.0	28.0	39.0
84-85	32.88653183191201	35.0	34.0	36.5	28.0	39.0
86-87	32.63910524946132	35.0	34.0	36.0	27.5	37.0
88-89	32.40335677516558	35.0	34.0	36.0	27.0	37.0
90-91	32.21391062373375	35.0	34.0	35.5	27.0	37.0
92-93	32.047772564927456	35.0	34.0	35.0	26.0	36.0
94-95	31.907119031181324	35.0	34.0	35.0	26.0	36.0
96-97	31.76324756121587	35.0	34.0	35.0	26.0	36.0
98-99	31.648233225720684	35.0	34.0	35.0	25.0	36.0
100	31.544337013689706	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.016386421326998857
1101	2	0.03165480260386744
1101	3	0.05163684145454539
1101	4	0.036432887238454725
1101	5	0.039260674861445466
1101	6	0.069842557531274
1101	7	0.05341180716822436
1101	8	0.05948031284986399
1101	9	0.06232379640476182
1101	10-11	0.11280199178349193
1101	12-13	0.07075332412442492
1101	14-15	0.11629824785016041
1101	16-17	0.06200909728400461
1101	18-19	0.035140261956165375
1101	20-21	0.03595042213702726
1101	22-23	0.09524505405474315
1101	24-25	0.08994857220954344
1101	26-27	0.11377256647592304
1101	28-29	0.08795061105340807
1101	30-31	0.06384498232442581
1101	32-33	0.008178364849811715
1101	34-35	0.0809452097919987
1101	36-37	0.02521483689655213
1101	38-39	0.023197900691378948
1101	40-41	-0.03084923487416802
1101	42-43	-0.06970002542060172
1101	44-45	-0.004570663595956148
1101	46-47	0.04226536770137557
1101	48-49	0.03601779850286135
1101	50-51	-0.07577049894628374
1101	52-53	0.0020002246895387543
1101	54-55	-0.0026407644720265466
1101	56-57	0.026244379327941658
1101	58-59	0.01056948151228454
1101	60-61	0.0031950477305429104
1101	62-63	-0.015509096312904092
1101	64-65	-0.015396272426947633
1101	66-67	-0.03690031285695028
1101	68-69	-0.04029672685336294
1101	70-71	-0.05022997166074816
1101	72-73	-0.08629631333953824
1101	74-75	-0.09236906056802496
1101	76-77	-0.06895657955455903
1101	78-79	-0.027829562939594155
1101	80-81	0.013856390116622208
1101	82-83	-0.026879964037568982
1101	84-85	-0.0076813944118896416
1101	86-87	0.03062111561994385
1101	88-89	0.04278116508486818
1101	90-91	-0.008876343897917138
1101	92-93	-0.05158852262722746
1101	94-95	-0.04171197346904165
1101	96-97	-0.02288657550437634
1101	98-99	-0.0671175940947002
1101	100	-0.08776430798253898
1102	1	-0.012511072906107756
1102	2	-0.04365430774779355
1102	3	0.013595289374684683
1102	4	0.04287527433036331
1102	5	-0.011256579537104017
1102	6	-0.016532959175059148
1102	7	-0.05043398598360227
1102	8	-0.0066128818506783205
1102	9	-0.01354053727852289
1102	10-11	2.777702227447776E-4
1102	12-13	-0.007743791211005657
1102	14-15	-0.09732708799373313
1102	16-17	-0.06724725969581868
1102	18-19	-0.02179646724709272
1102	20-21	-0.03193411759546194
1102	22-23	0.03006469705542969
1102	24-25	0.028516239120612852
1102	26-27	-0.0058274413188215135
1102	28-29	0.03206229026068286
1102	30-31	0.00354920524925717
1102	32-33	0.033910005180793235
1102	34-35	-0.013361307589065063
1102	36-37	-0.02737487574530917
1102	38-39	-0.013532855423235901
1102	40-41	-0.08331350084060318
1102	42-43	-0.0019195424874354217
1102	44-45	-0.009774112526976353
1102	46-47	-0.06128203287300238
1102	48-49	-0.04054882342521893
1102	50-51	-0.03946723587120715
1102	52-53	-0.02792619109190042
1102	54-55	-0.048480182375094216
1102	56-57	-0.053545872003873285
1102	58-59	-0.013306872520153945
1102	60-61	-0.04651456757443384
1102	62-63	-0.10224991556495411
1102	64-65	-0.12316767874506951
1102	66-67	-0.044405571264924504
1102	68-69	-0.03388912685531409
1102	70-71	-0.035798275548621916
1102	72-73	-0.031686132793765864
1102	74-75	-0.05132950041086559
1102	76-77	-0.03673916341504935
1102	78-79	0.018673321724975267
1102	80-81	-0.041604361516000665
1102	82-83	-0.057507526255463404
1102	84-85	-0.09309345324759732
1102	86-87	-0.12744862237919108
1102	88-89	-0.08082468694164646
1102	90-91	-0.09576811021146625
1102	92-93	-0.1098525659562668
1102	94-95	-0.05465604556556514
1102	96-97	0.008038846447085746
1102	98-99	-0.05104405372157572
1102	100	-0.05785014761053375
1103	1	-0.1133417334922413
1103	2	-0.11736732045557119
1103	3	-0.054395678936778324
1103	4	-0.05250919019164968
1103	5	-0.08923630416797579
1103	6	-0.041664361271521955
1103	7	-0.0968203307294857
1103	8	-0.04489314375617681
1103	9	-0.018262416173492113
1103	10-11	-0.014927187346295057
1103	12-13	-0.006524079985588571
1103	14-15	-0.09193794090131036
1103	16-17	-0.050174670744972616
1103	18-19	-0.08538884692830351
1103	20-21	-0.06966959148429908
1103	22-23	-0.06575284973084905
1103	24-25	-0.06051806819043293
1103	26-27	-0.05670563187012334
1103	28-29	-0.05855614508869422
1103	30-31	-0.006937892305742821
1103	32-33	-0.022748139927287525
1103	34-35	-0.02541737626002316
1103	36-37	0.012900932780809171
1103	38-39	-0.018877488624951866
1103	40-41	-0.08645603607929786
1103	42-43	0.01870026387446444
1103	44-45	-0.007897639607115536
1103	46-47	-0.011169649652117641
1103	48-49	0.01382018624138226
1103	50-51	-0.05999267508131112
1103	52-53	0.026782165225604615
1103	54-55	0.07365284365545932
1103	56-57	0.007808201510876245
1103	58-59	-0.002113026046920652
1103	60-61	-0.03499907666391522
1103	62-63	-0.05158179766124249
1103	64-65	-0.010416577150671458
1103	66-67	0.021553144646738076
1103	68-69	0.03715305241004074
1103	70-71	0.10060480152096574
1103	72-73	0.10820606015110457
1103	74-75	0.12376458733302087
1103	76-77	0.15264018970280802
1103	78-79	0.12665018414712392
1103	80-81	0.17469018356887744
1103	82-83	0.09636616207748716
1103	84-85	0.10403361335666261
1103	86-87	0.15294047112814724
1103	88-89	0.17515833592739938
1103	90-91	0.1398081452601474
1103	92-93	0.07996681634862313
1103	94-95	0.09104402358980934
1103	96-97	0.10177178055626968
1103	98-99	0.16754988451993924
1103	100	0.24604721767236626
1104	1	-0.15798545002675013
1104	2	-0.060923322631921906
1104	3	-0.05635721377804259
1104	4	-0.04246170201816568
1104	5	-0.0905320052607479
1104	6	-0.08722461408499527
1104	7	-0.08332033332832367
1104	8	-0.1252342752357265
1104	9	-0.10947261017543752
1104	10-11	-0.0821012952077993
1104	12-13	-0.07361853440236388
1104	14-15	-0.06979640786520491
1104	16-17	0.0032896925997789594
1104	18-19	-0.04177574998226419
1104	20-21	-0.005157737348248759
1104	22-23	-0.003694450685799211
1104	24-25	-0.055106755747125646
1104	26-27	-0.08759364049264917
1104	28-29	-0.06281165740488603
1104	30-31	-0.030775025045791438
1104	32-33	-0.05927661403249118
1104	34-35	-0.09518844440646035
1104	36-37	-0.07328500495458456
1104	38-39	-0.06642628902206837
1104	40-41	-0.12264148250147855
1104	42-43	-0.1677646183653465
1104	44-45	-0.09793964631849406
1104	46-47	-0.06542517904097167
1104	48-49	-0.0353268048483244
1104	50-51	-0.15515205993590797
1104	52-53	-0.08813079968047788
1104	54-55	-0.05109854521493418
1104	56-57	-0.10341050529884654
1104	58-59	-0.1101762499775063
1104	60-61	-0.08590550023714627
1104	62-63	-0.11052813529297367
1104	64-65	0.02279115072447979
1104	66-67	-0.08294477668101052
1104	68-69	-0.1157361671573014
1104	70-71	-0.13170074249157437
1104	72-73	-0.11689406246474476
1104	74-75	-0.12076928749957005
1104	76-77	-0.09433528420672843
1104	78-79	-0.10230475544960882
1104	80-81	-0.11833668564935351
1104	82-83	-0.11649807980857219
1104	84-85	-0.15623133308393733
1104	86-87	-0.11160686758368144
1104	88-89	-0.11884431170074805
1104	90-91	-0.09098891540444498
1104	92-93	-0.0877086738102939
1104	94-95	-0.13713226991365346
1104	96-97	-0.12032674075786787
1104	98-99	-0.1221072019941758
1104	100	-0.025617087086608592
1105	1	-0.15371429799854752
1105	2	-0.1322360324366656
1105	3	-0.11903308986489947
1105	4	-0.02878535689703199
1105	5	-0.07290446334553735
1105	6	0.0023043868885750385
1105	7	-0.0018147208979044649
1105	8	-8.153964237749278E-4
1105	9	-0.010487104309426343
1105	10-11	-0.0021556104228395156
1105	12-13	-0.05604911825271586
1105	14-15	-0.10386219109803818
1105	16-17	-0.11927582509542844
1105	18-19	-0.17657755072149683
1105	20-21	-0.09245877848085371
1105	22-23	-0.12197801188731461
1105	24-25	-0.09740134412918167
1105	26-27	-0.022104669348443906
1105	28-29	-0.02132770510430504
1105	30-31	-0.03975453349230662
1105	32-33	-0.140064576547843
1105	34-35	-0.07399369715358972
1105	36-37	-0.06487649556838448
1105	38-39	-0.053824160000267796
1105	40-41	-0.08406549373570726
1105	42-43	-0.15932377087511895
1105	44-45	-0.11703032744441799
1105	46-47	-0.17092897752586822
1105	48-49	-0.1250483850665276
1105	50-51	-0.12201431252541539
1105	52-53	-0.1687083951116719
1105	54-55	-0.15353396961640442
1105	56-57	-0.11378612385920661
1105	58-59	-0.15416456067523399
1105	60-61	-0.07865688974976592
1105	62-63	-0.16186504484164743
1105	64-65	-0.131345278654166
1105	66-67	-0.16142052711230548
1105	68-69	-0.13157156071146403
1105	70-71	-0.05666869896922577
1105	72-73	-0.0026929327844626982
1105	74-75	-0.06510257311004608
1105	76-77	-0.10359676285015951
1105	78-79	0.036331501151693146
1105	80-81	0.07211038995642127
1105	82-83	-0.025255234614085964
1105	84-85	-0.011221138298999733
1105	86-87	-0.06294035509083784
1105	88-89	-0.054662417357207005
1105	90-91	-0.06939585230798428
1105	92-93	-0.10452239853833589
1105	94-95	-0.06974670096762736
1105	96-97	-0.12509184885465885
1105	98-99	-0.22536288108664237
1105	100	-0.21843147002118357
1106	1	-0.12249282638341441
1106	2	-0.09540860241355631
1106	3	-0.08038723912411427
1106	4	-0.014759745821372405
1106	5	-0.008490346717820785
1106	6	-0.08883311476738953
1106	7	-0.05486286923525796
1106	8	-0.03706731611439551
1106	9	-0.056733284770878356
1106	10-11	-0.025994679233271256
1106	12-13	-0.04740322277566378
1106	14-15	-0.02531080313394085
1106	16-17	-0.10171778124176711
1106	18-19	-0.0653833408056883
1106	20-21	-0.05312842689136943
1106	22-23	-0.08481812651604059
1106	24-25	-0.08134643859629165
1106	26-27	-0.18018397649057505
1106	28-29	-0.10417368379079761
1106	30-31	-0.12737848900887627
1106	32-33	-0.09540288945979825
1106	34-35	-0.10695162111407086
1106	36-37	-0.07379273875584857
1106	38-39	-0.0881044914006992
1106	40-41	-0.1583493852765514
1106	42-43	-0.20207523771934888
1106	44-45	-0.20760202365000424
1106	46-47	-0.1310609429975571
1106	48-49	-0.05632261299806629
1106	50-51	-0.17363357030180993
1106	52-53	-0.11103974879336675
1106	54-55	-0.09986129948801903
1106	56-57	-0.09880840612422048
1106	58-59	-0.09300266188366635
1106	60-61	-0.09669382804634097
1106	62-63	-0.06946262544700232
1106	64-65	-0.03180444945748917
1106	66-67	-0.09375747571265691
1106	68-69	-0.17111570832842204
1106	70-71	-0.040296472653402304
1106	72-73	-0.03450308306654648
1106	74-75	-0.04928036828204796
1106	76-77	-0.08320575928308926
1106	78-79	-0.03201002613266013
1106	80-81	-0.011387637751774093
1106	82-83	-0.04522037785237387
1106	84-85	-0.09308955755914639
1106	86-87	-0.04682815828361697
1106	88-89	-0.07146665523750073
1106	90-91	-0.13636000788510927
1106	92-93	-0.08476381776298325
1106	94-95	-0.15115790843968213
1106	96-97	-0.1633622577527305
1106	98-99	-0.10630652609893687
1106	100	-0.11112758295974601
1107	1	-0.30997204630993025
1107	2	-0.21870875747830354
1107	3	-0.17019361773355968
1107	4	-0.2393238182966826
1107	5	-0.3209328959264326
1107	6	-0.32390415118664606
1107	7	-0.23947367068252845
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2216	22-23	-0.14929032547594545
2216	24-25	-0.13217161098261698
2216	26-27	-0.07588787207350123
2216	28-29	-0.10124393251938102
2216	30-31	-0.119446681803133
2216	32-33	-0.025491883070969834
2216	34-35	-0.0032546708576361993
2216	36-37	0.005666476960819011
2216	38-39	0.04840295517191606
2216	40-41	0.03893397378287489
2216	42-43	0.11671599309282499
2216	44-45	0.12166312514151656
2216	46-47	0.14488791734027018
2216	48-49	0.18357787870392173
2216	50-51	0.1258843974160797
2216	52-53	0.12986014048039607
2216	54-55	0.1841242727326815
2216	56-57	0.11309271778655017
2216	58-59	0.1511829549002286
2216	60-61	0.09862136857346115
2216	62-63	0.10656670516517863
2216	64-65	0.11219657276884476
2216	66-67	0.1331153261777942
2216	68-69	0.13048600085984674
2216	70-71	0.17244989860456172
2216	72-73	0.13593476994957854
2216	74-75	0.16973691663600476
2216	76-77	0.21214248298275606
2216	78-79	0.1587988889439771
2216	80-81	0.21625909962754974
2216	82-83	0.23393334503925445
2216	84-85	0.26010323131398394
2216	86-87	0.2386063901679023
2216	88-89	0.2525776582203676
2216	90-91	0.20232243242642056
2216	92-93	0.2493286953350804
2216	94-95	0.16075105529048273
2216	96-97	0.21641541250410512
2216	98-99	0.21896336461720622
2216	100	0.26423417895803425
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52.0
3	320.0
4	70.0
5	139.0
6	340.0
7	733.0
8	1255.0
9	2406.0
10	3663.0
11	4814.0
12	7377.0
13	10922.0
14	12224.0
15	16213.0
16	21379.0
17	32425.0
18	37508.0
19	19240.0
20	11486.0
21	10472.0
22	10178.0
23	10072.0
24	10768.0
25	12234.0
26	14923.0
27	19003.0
28	23745.0
29	29787.0
30	37470.0
31	47376.0
32	60349.0
33	77776.0
34	108928.0
35	184505.0
36	291333.0
37	592254.0
38	1082003.0
39	729663.0
40	7549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73294489871899	21.22888691240491	15.031725202363011	22.006442986513083
2	14.103065302052087	22.287139799417577	41.11660239986474	22.4931924986656
3	16.36355014808419	28.987300577462015	32.38839634120698	22.260752933246813
4	10.804458652299747	18.916813483889435	40.275741655127334	30.00298620868349
5	10.993498885890382	39.39432872883636	35.648034544598694	13.964137840674558
6	26.331219654559447	40.81249714221523	18.822598317675023	14.033684885550308
7	23.365953946904195	35.86761216769961	23.881201957462615	16.88523192793358
8	24.580053819496385	35.400798316884725	21.342811676358203	18.676336187260688
9	25.712618823901984	15.964620473373484	22.594598347762446	35.72816235496209
10-11	22.450404380073802	27.837815562945494	29.796096703485286	19.915683353495417
12-13	24.253969992271983	26.30271236939571	30.052436469680387	19.390881168651923
14-15	21.49617240302866	27.187059160237474	29.17134967035982	22.145418766374046
16-17	18.814525957717766	30.514127476676244	29.686075517774153	20.985271047831837
18-19	18.764968998270508	27.873246781105284	34.12525870055935	19.236525520064863
20-21	20.44138873945301	26.829913682198526	34.002798229951615	18.725899348396847
22-23	19.944972456592	26.93566637773631	34.161277329956114	18.958083835715573
24-25	19.58377658410936	27.01480939769734	34.06785243834734	19.333561579845966
26-27	19.360450495025752	27.08778878148615	34.06724684281193	19.48451388067617
28-29	19.267587227866066	27.079218644927707	34.263759200496196	19.389434926710035
30-31	19.5627191594101	27.349852474237146	33.84399201736524	19.243436348987515
32-33	19.319105240711202	27.22732272487741	34.157611694610374	19.295960339801017
34-35	19.571411938623942	27.186375616106805	33.775530725074354	19.466681720194895
36-37	19.798134695604485	27.149597773805525	33.4376403332066	19.614627197383392
38-39	19.631754999346583	27.275507470829798	33.498719838731574	19.59401769109205
40-41	19.58507129377642	27.201938725569057	33.484746874493716	19.728243106160807
42-43	19.647534717375912	27.327053127347543	33.144370176044944	19.8810419792316
44-45	19.96201185058653	27.504066529925513	32.700771419950684	19.83315019953728
46-47	20.129832557519897	27.81631285584773	32.075167346514824	19.97868724011755
48-49	20.269690512143836	27.837155477779817	31.489902482394616	20.403251527681736
50-51	20.197536537104288	28.01897972008836	31.285278791805503	20.498204951001846
52-53	20.179888162981786	28.215765878094746	31.19847210926231	20.405873849661162
54-55	20.34658139988786	28.420459454413233	31.005940157545208	20.2270189881537
56-57	20.242459139707552	28.149393217071495	30.964276087290937	20.64387155593002
58-59	20.127318403405546	27.909623352545783	31.354443082890327	20.608615161158347
60-61	20.286200287419625	28.09320095791489	31.116653332499745	20.503945422165746
62-63	20.14730859796452	27.92155584298467	31.39912825712816	20.532007301922647
64-65	20.155031320413773	27.815937070707953	31.455557415301662	20.57347419357661
66-67	20.104230011655595	27.910656522342293	31.283440685522322	20.70167278047979
68-69	20.441762681476952	28.11371788933663	30.867445510695486	20.577073918490925
70-71	20.369178643987233	27.86944819367837	30.906257109196694	20.8551160531377
72-73	20.305166986057223	27.98348154099463	31.05231226390584	20.659039209042305
74-75	20.325524629776453	28.165087793228594	30.77341136297756	20.73597621401739
76-77	20.374670805056898	28.065452161188354	30.6654095127936	20.89446752096115
78-79	20.42052882932722	28.0703910898355	30.81771533085703	20.69136474998024
80-81	20.261623217801024	28.123670323302573	30.857475028715726	20.757231430180674
82-83	20.187299016537626	28.09675770437194	31.035219542458403	20.680723736632032
84-85	20.140635924115422	28.028127749331272	30.76175863492889	21.06947769162442
86-87	20.254053127948694	28.044788972590386	30.69906948610467	21.002088413356248
88-89	20.13330290635505	27.922496446069662	30.78290054626023	21.161300101315064
90-91	20.068040027497137	27.946454818483485	30.679797663759594	21.305707490259785
92-93	20.071952223407532	27.98666857282291	30.70058439306823	21.240794810701328
94-95	19.903020795390926	27.888147207201282	30.846607089707952	21.36222490769984
96-97	19.901725865744798	28.02209968454223	30.684121253312746	21.39205319640023
98-99	19.88645304273471	27.88639207641	30.70032666377735	21.526828217077938
100	19.92882753123604	27.96251590838464	30.539300237119253	21.56935632326007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4596.0
1	2623.0
2	2582.0
3	4509.0
4	2793.5
5	1582.0
6	3850.5
7	10220.5
8	17798.0
9	19108.0
10	12002.0
11	5498.0
12	4528.0
13	4851.0
14	5338.5
15	5814.0
16	6320.0
17	7046.5
18	7908.0
19	8806.0
20	10216.0
21	12530.0
22	16265.5
23	21886.0
24	27976.0
25	34255.5
26	42672.0
27	53494.0
28	67405.5
29	79103.5
30	85496.0
31	92882.5
32	100536.0
33	107049.0
34	114009.5
35	119314.5
36	120582.0
37	120990.0
38	120652.5
39	118391.5
40	117510.0
41	114868.5
42	108789.0
43	104403.0
44	101810.0
45	98754.5
46	96860.5
47	94890.0
48	92069.0
49	90489.0
50	90585.5
51	88076.5
52	82325.5
53	77662.5
54	74444.5
55	71351.0
56	65111.5
57	57572.5
58	52594.5
59	48735.0
60	43020.0
61	35901.5
62	30206.0
63	24697.5
64	19067.0
65	14823.5
66	11712.5
67	8873.0
68	6672.0
69	5067.5
70	3525.5
71	2509.0
72	1903.0
73	1403.0
74	1176.5
75	901.5
76	514.0
77	373.0
78	281.5
79	139.5
80	50.0
81	18.5
82	9.5
83	5.0
84	4.0
85	3.5
86	3.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005278081510513543
2	0.004826480953464256
3	0.0010443262881764763
4	0.0
5	0.044059279347121075
6	0.0
7	0.0
8	0.0
9	2.8225034815580443E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.8346272630127288E-4
20-21	0.0
22-23	2.8225034815580443E-5
24-25	2.8225034815580443E-5
26-27	1.9757524370906312E-4
28-29	1.8346272630127288E-4
30-31	3.8103797001033603E-4
32-33	0.0016088269844880855
34-35	0.0011713389448465886
36-37	0.0013265766363322808
38-39	0.0011572264274387982
40-41	2.2580027852464355E-4
42-43	6.632883181661404E-4
44-45	1.9757524370906312E-4
46-47	1.6935020889348267E-4
48-49	1.1290013926232177E-4
50-51	4.0926300482591646E-4
52-53	8.608635618752035E-4
54-55	9.737637011375253E-4
56-57	0.0025120280985866596
58-59	7.197383877973014E-4
60-61	0.0014253642581868124
62-63	0.001453589293002393
64-65	0.0016088269844880855
66-67	5.786132137193991E-4
68-69	3.104753829713849E-4
70-71	2.2580027852464355E-4
72-73	2.3991279593243376E-4
74-75	7.620759400206721E-4
76-77	8.608635618752035E-4
78-79	9.59651183729735E-4
80-81	9.737637011375253E-4
82-83	0.001778177193381568
84-85	0.0013265766363322808
86-87	0.0020604275415373728
88-89	0.0012842390841089101
90-91	0.0011148888752154276
92-93	0.0013406891537400712
94-95	8.326385270596232E-4
96-97	2.1168776111685336E-4
98-99	4.0926300482591646E-4
100	3.104753829713849E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3542954.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.62490217119397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.90732361314943	42.589022929425106
2	15.87068704711034	18.290935769587374
3	4.96308856984817	8.579924799134153
4	2.024514387187939	4.6664977402351875
5	1.0280169713687284	2.9619688702725044
6	0.5783712922338079	1.9997153480160135
7	0.35362460795994416	1.4264308407313024
8	0.23620111953455472	1.0888853124724167
9	0.15684196511978368	0.8134202606728816
>10	0.799128807030546	7.99599765588475
>50	0.048776716387525315	1.9231409800981207
>100	0.029744523634590788	3.281041751695262
>500	0.0023554428486783074	0.8854544235113729
>1k	0.0010305062335918451	0.958976002427679
>5k	1.4721517622740639E-4	0.46375155367599247
>10k+	1.4721517622740639E-4	2.0748357621598945
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31282	0.8829355391009874	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24863	0.7017590406197766	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17229	0.4862891248376354	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5755	0.16243507536366547	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5405	0.1525563131782123	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5240	0.14789918243364153	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4856	0.13706076906445866	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4444	0.1254320547204395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.8225034815580443E-5	0.0
3	0.0	0.0	0.0	2.8225034815580443E-5	0.0
4	0.0	0.0	0.0	2.8225034815580443E-5	0.0
5	0.0	0.0	0.0	2.8225034815580443E-5	0.0
6	0.0	0.0	0.0	2.8225034815580443E-5	0.0
7	0.0	0.0	0.0	2.8225034815580443E-5	0.0
8	0.0	0.0	0.0	2.8225034815580443E-5	0.0
9	0.0	0.0	0.0	2.8225034815580443E-5	0.0
10-11	0.0	0.0	0.0	4.233755222337067E-5	0.0
12-13	0.0	0.0	0.0	5.645006963116089E-5	0.0
14-15	0.0	0.0	0.0	5.645006963116089E-5	0.0
16-17	0.0	0.0	0.0	5.645006963116089E-5	0.0
18-19	0.0	0.0	0.0	5.645006963116089E-5	2.8225034815580443E-5
20-21	0.0	0.0	0.0	5.645006963116089E-5	4.233755222337067E-5
22-23	2.8225034815580443E-5	0.0	0.0	2.399127959324338E-4	5.645006963116089E-5
24-25	2.8225034815580443E-5	0.0	0.0	6.632883181661405E-4	5.645006963116089E-5
26-27	2.8225034815580443E-5	0.0	0.0	0.0011148888752154276	5.645006963116089E-5
28-29	2.8225034815580443E-5	0.0	0.0	0.0026249282378489813	5.645006963116089E-5
30-31	2.8225034815580443E-5	0.0	0.0	0.006505870524991292	5.645006963116089E-5
32-33	2.8225034815580443E-5	0.0	0.0	0.013646804333333144	5.645006963116089E-5
34-35	5.645006963116089E-5	0.0	0.0	0.023892491971388846	5.645006963116089E-5
36-37	5.645006963116089E-5	0.0	0.0	0.04057348754739689	5.645006963116089E-5
38-39	5.645006963116089E-5	0.0	0.0	0.06617359412512835	5.645006963116089E-5
40-41	5.645006963116089E-5	0.0	0.0	0.09321317747845442	5.645006963116089E-5
42-43	5.645006963116089E-5	0.0	0.0	0.1193777847524975	5.645006963116089E-5
44-45	7.056258703895111E-5	0.0	0.0	0.15173778716856046	8.467510444674133E-5
46-47	8.467510444674133E-5	1.4112517407790222E-5	0.0	0.19387776414822205	1.1290013926232177E-4
48-49	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.23896725726611184	1.1290013926232177E-4
50-51	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.2878530175666972	1.1290013926232177E-4
52-53	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.335765014166145	1.1290013926232177E-4
54-55	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.39088850716097356	1.1290013926232177E-4
56-57	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.45635647541571245	1.1290013926232177E-4
58-59	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.5340317712281899	1.1290013926232177E-4
60-61	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.6170415986208119	1.1290013926232177E-4
62-63	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.7026622417338753	1.1290013926232177E-4
64-65	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.7932787160092962	1.1290013926232177E-4
66-67	8.467510444674133E-5	2.8225034815580443E-5	0.0	0.9005338483085019	1.1290013926232177E-4
68-69	1.9757524370906312E-4	2.8225034815580443E-5	0.0	1.0244699761837155	1.1290013926232177E-4
70-71	1.9757524370906312E-4	2.8225034815580443E-5	0.0	1.1656798253660647	1.1290013926232177E-4
72-73	1.9757524370906312E-4	2.8225034815580443E-5	0.0	1.312986282068579	1.1290013926232177E-4
74-75	1.9757524370906312E-4	2.8225034815580443E-5	0.0	1.4594742127614415	1.1290013926232177E-4
76-77	1.9757524370906312E-4	2.8225034815580443E-5	0.0	1.6212459998069408	1.1290013926232177E-4
78-79	1.9757524370906312E-4	2.8225034815580443E-5	0.0	1.8148697386418227	1.1290013926232177E-4
80-81	1.9757524370906312E-4	2.8225034815580443E-5	0.0	2.0358153111781863	1.1290013926232177E-4
82-83	1.9757524370906312E-4	2.8225034815580443E-5	0.0	2.2723552154501583	1.1290013926232177E-4
84-85	1.9757524370906312E-4	2.8225034815580443E-5	0.0	2.506354866588728	1.1290013926232177E-4
86-87	1.9757524370906312E-4	2.8225034815580443E-5	0.0	2.7570355133033058	1.1290013926232177E-4
88	1.9757524370906312E-4	2.8225034815580443E-5	0.0	2.9659431084908245	1.1290013926232177E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10205	0.0	66.03625	1
GTATCAA	21205	0.0	47.37096	1
GTGGTAT	3060	0.0	38.26724	1
TATCAAC	27665	0.0	36.15392	2
ATCAACG	27650	0.0	36.035442	3
TCAACGC	27705	0.0	35.928944	4
TGGTATC	3225	0.0	35.577766	2
CAACGCA	28940	0.0	34.460686	5
AACGCAG	29985	0.0	33.337788	6
CGCAGAG	33255	0.0	29.890057	8
ACGCAGA	33500	0.0	29.741602	7
GCAGAGT	35535	0.0	27.839994	9
GTACATG	17990	0.0	27.369366	1
CATGGGT	8195	0.0	27.081629	4
TACATGG	18350	0.0	26.112995	2
ACATGGG	18370	0.0	25.238398	3
GAGTACT	25780	0.0	23.854162	12-13
CAGAGTA	35305	0.0	23.149239	10-11
AGAGTAC	34280	0.0	22.724068	10-11
TATAACG	270	4.7293724E-11	22.641134	2
>>END_MODULE