FastQCFastQC Report
Wed 25 May 2016
SRR2431411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7603
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA811.0653689333157963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.6444824411416545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.381428383532816No Hit
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT150.19729054320662895No Hit
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC140.18413784032618705No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT140.18413784032618705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1709851374457451No Hit
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGG130.1709851374457451No Hit
ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAA120.15783243456530316No Hit
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG110.14467973168486123No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.14467973168486123No Hit
CCCACAGGCCGGTGGCATCGGCGCGGAGTGCCCAGGTTCACAGTGCAGCT100.1315270288044193No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC100.1315270288044193No Hit
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG100.1315270288044193No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG100.1315270288044193RNA PCR Primer, Index 27 (96% over 30bp)
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC90.11837432592397738No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.10522162304353545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTCA250.005038714556.3999986
GTATCAA405.3263E-446.9999961
TCAACGC405.3263E-446.9999964
TATCAAC405.3263E-446.9999962
ATCAACG405.3263E-446.9999963
CAACGCA405.3263E-446.9999965
AACGCAG459.516354E-441.777786
ACGCAGA459.516354E-441.777787
CGCAGAG500.001597869437.68
GCAGAGT600.00390855231.3333349
GAGTACT601.7246399E-423.512-13
CAGAGTA757.788353E-418.80000110-11
AGTACTT650.007689140318.07692312-13
AGAGTAC650.007689140318.07692310-11
Produced by FastQC (version 0.11.5)