##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431411_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7603 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.558726818361173 31.0 31.0 34.0 30.0 34.0 2 31.746810469551495 31.0 31.0 34.0 30.0 34.0 3 31.884124687623306 31.0 31.0 34.0 30.0 34.0 4 35.71629619886887 37.0 35.0 37.0 35.0 37.0 5 35.481520452452976 37.0 35.0 37.0 33.0 37.0 6 35.41601999210838 37.0 35.0 37.0 33.0 37.0 7 35.369854004998025 37.0 35.0 37.0 33.0 37.0 8 35.295146652637115 37.0 35.0 37.0 32.0 37.0 9 36.92292516112061 39.0 37.0 39.0 33.0 39.0 10-11 36.95666184400895 39.0 37.0 39.0 32.5 39.0 12-13 36.908325660923325 39.0 37.0 39.0 32.5 39.0 14-15 38.103117190582665 40.0 37.0 41.0 33.0 41.0 16-17 38.14027357621991 40.0 37.0 41.0 33.0 41.0 18-19 38.146849927660135 40.0 37.0 41.0 33.0 41.0 20-21 38.095554386426414 40.0 37.0 41.0 33.0 41.0 22-23 37.91937393134289 40.0 37.0 41.0 32.5 41.0 24-25 37.727212942259634 40.0 37.0 41.0 32.0 41.0 26-27 37.584900697093246 40.0 36.5 41.0 32.0 41.0 28-29 37.33138234907274 40.0 36.0 41.0 31.5 41.0 30-31 37.16118637379982 40.0 36.0 41.0 31.0 41.0 32-33 36.83670919373931 39.0 35.0 41.0 30.5 41.0 34-35 36.558332237274755 39.0 35.0 41.0 30.0 41.0 36-37 36.27002499013547 39.0 35.0 41.0 30.0 41.0 38-39 36.06405366302775 39.0 35.0 41.0 29.5 41.0 40-41 35.90142049191109 39.0 35.0 41.0 28.0 41.0 42-43 35.71991319216099 39.0 35.0 41.0 27.5 41.0 44-45 35.452518742601605 39.0 35.0 41.0 26.5 41.0 46-47 35.05760883861633 39.0 34.5 41.0 25.0 41.0 48-49 34.707681178482176 38.0 34.0 41.0 23.5 41.0 50-51 34.46389583059319 38.0 34.0 40.0 23.5 41.0 52-53 34.322175457056424 38.0 34.0 40.0 23.0 41.0 54-55 34.13935288701828 38.0 33.5 40.0 23.0 41.0 56-57 33.89997369459424 37.5 33.0 40.0 21.5 41.0 58-59 33.61929501512561 37.0 33.0 40.0 20.5 41.0 60-61 33.31092989609365 37.0 33.0 40.0 19.0 41.0 62-63 33.098842562146515 36.5 33.0 40.0 16.5 41.0 64-65 32.80889122714718 36.0 33.0 40.0 14.0 41.0 66-67 32.5774036564514 36.0 32.5 39.0 13.0 41.0 68-69 32.272458240168355 35.0 32.0 39.0 12.0 41.0 70-71 31.9613968170459 35.0 32.0 39.0 9.5 41.0 72-73 31.734052347757462 35.0 32.0 38.0 9.0 40.0 74-75 31.397671971590164 35.0 31.5 37.0 7.0 40.0 76-77 31.188215178219124 35.0 31.0 37.0 7.0 39.0 78-79 30.9018150729975 35.0 31.0 36.0 3.5 39.0 80-81 30.66158095488623 35.0 31.0 36.0 2.0 39.0 82-83 30.511048270419572 35.0 31.0 36.0 2.0 37.0 84-85 30.273313165855583 35.0 31.0 35.0 2.0 37.0 86-87 30.032750230172297 35.0 31.0 35.0 2.0 37.0 88-89 29.791069314744178 35.0 31.0 35.0 2.0 36.0 90-91 29.639681704590295 35.0 31.0 35.0 2.0 36.0 92-93 29.524792844929635 35.0 31.0 35.0 2.0 36.0 94-95 29.376627646981454 35.0 31.0 35.0 2.0 36.0 96-97 29.159081941338947 34.0 31.0 35.0 2.0 35.0 98-99 28.989083256609234 34.0 31.0 35.0 2.0 35.0 100 28.845718795212417 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.0212343429140347 1101 2 -1.156765977078475 1101 3 -0.8662350019771914 1101 4 -1.2400543250152651 1101 5 -2.108661217254962 1101 6 -0.13042378628315987 1101 7 0.05348494821151206 1101 8 -2.35975669295982 1101 9 -0.6001003770535007 1101 10-11 0.439786667325734 1101 12-13 -2.191168423590291 1101 14-15 0.6523082061167926 1101 16-17 -1.6335777286949167 1101 18-19 0.5501706843503698 1101 20-21 -1.3143040228001226 1101 22-23 -1.2405606437832972 1101 24-25 -0.5611408123126864 1101 26-27 -2.6278966693029133 1101 28-29 -0.6839321160610083 1101 30-31 -0.3435017022907658 1101 32-33 0.5046870880855394 1101 34-35 2.07090378154831 1101 36-37 1.2527403694396 1101 38-39 2.463739060418746 1101 40-41 2.00414020658161 1101 42-43 0.5599785305839973 1101 44-45 0.44621735686188657 1101 46-47 2.5373353100892118 1101 48-49 2.626223375149152 1101 50-51 3.552136090905634 1101 52-53 3.6769472931582357 1101 54-55 4.084132317335424 1101 56-57 3.4251054067460345 1101 58-59 2.17545954479548 1101 60-61 3.031137352231106 1101 62-63 2.496010543276171 1101 64-65 3.9785081671800455 1101 66-67 3.7911466463224386 1101 68-69 3.1503303228889195 1101 70-71 2.92487115748834 1101 72-73 3.072545510045501 1101 74-75 3.651337594696969 1101 76-77 3.2790256401584514 1101 78-79 3.5425751765595663 1101 80-81 2.6406374007936435 1101 82-83 4.001525763732808 1101 84-85 5.064496484418356 1101 86-87 4.604535449359677 1101 88-89 3.1295131300990633 1101 90-91 3.2747470953525593 1101 92-93 2.8002526444909286 1101 94-95 4.98894163562132 1101 96-97 4.332851350038855 1101 98-99 2.126451066294809 1101 100 2.1327013481310324 1102 1 -0.6176545459748546 1102 2 -0.18454375485625008 1102 3 -0.03290166864385924 1102 4 -0.1011654361263723 1102 5 -0.08088343947718357 1102 6 -0.2693126751720456 1102 7 -0.02984838512182364 1102 8 -0.2486455818487059 1102 9 -0.07232259927572215 1102 10-11 -1.1435466660075946 1102 12-13 -0.6217239791458482 1102 14-15 -0.028247349438764502 1102 16-17 -1.0641332842504667 1102 18-19 -0.005384871205180275 1102 20-21 0.25514042164432027 1102 22-23 0.32888380066115275 1102 24-25 0.6194147432428707 1102 26-27 0.4137699973637581 1102 28-29 0.2605123283834345 1102 30-31 0.8926094088203484 1102 32-33 0.03246486586331798 1102 34-35 1.5014593371038671 1102 36-37 -1.6222596305604 1102 38-39 -2.5084831618034755 1102 40-41 -1.7736375711961685 1102 42-43 0.05997853058399727 1102 44-45 -0.7065604209158991 1102 46-47 -0.12933135657745964 1102 48-49 1.0012233751491522 1102 50-51 2.06602497979452 1102 52-53 -1.8786082623973215 1102 54-55 -1.6519787937756831 1102 56-57 0.2584387400793773 1102 58-59 -0.38009601076007726 1102 60-61 0.18391513000889148 1102 62-63 0.7043438766095065 1102 64-65 1.006285944957824 1102 66-67 1.7078133129891029 1102 68-69 0.15033032288891945 1102 70-71 2.008204490821676 1102 72-73 1.433656621156615 1102 74-75 1.262448705808076 1102 76-77 0.0984700846028943 1102 78-79 1.1675751765595628 1102 80-81 0.3906374007936435 1102 82-83 -0.7068075696005316 1102 84-85 -0.1438368489149724 1102 86-87 -0.03435343952921599 1102 88-89 -0.6760424254564903 1102 90-91 -1.9196973490918836 1102 92-93 -2.1858584666201857 1102 94-95 -0.23328058660090178 1102 96-97 -2.5282597610722597 1102 98-99 -2.234660044816298 1102 100 -2.8117430963134105 1103 1 1.1323454540251419 1103 2 1.1321229118104164 1103 3 1.4004316646894743 1103 4 0.5821678972069577 1103 5 0.7357832271894864 1103 6 1.1140206581612873 1103 7 0.7868182815448463 1103 8 1.0846877514846298 1103 9 1.1110107340576079 1103 10-11 0.3397866673257397 1103 12-13 0.8866093541874847 1103 14-15 0.2634193172278998 1103 16-17 0.8442000490828647 1103 18-19 1.0612817954614826 1103 20-21 1.0968070883109888 1103 22-23 1.4038838006611485 1103 24-25 1.8610814099095307 1103 26-27 1.7721033306970924 1103 28-29 2.160512328383426 1103 30-31 1.3676094088203499 1103 32-33 1.5491315325299837 1103 34-35 2.19312600377053 1103 36-37 2.2194070361062685 1103 38-39 2.5415168381965216 1103 40-41 2.5263624288038287 1103 42-43 3.12664519725066 1103 44-45 3.4351062457507737 1103 46-47 4.137335310089206 1103 48-49 3.692890041815822 1103 50-51 3.2743583131278555 1103 52-53 4.188058404269341 1103 54-55 4.539687872890987 1103 56-57 3.75843874007937 1103 58-59 4.353237322573257 1103 60-61 4.908915130008886 1103 62-63 2.996010543276178 1103 64-65 3.322952611624487 1103 66-67 2.7578133129891 1103 68-69 3.483663656222255 1103 70-71 2.9582044908216787 1103 72-73 3.0169899544899437 1103 74-75 2.5957820391414117 1103 76-77 2.8234700846028886 1103 78-79 2.3425751765595635 1103 80-81 2.5073040674603106 1103 82-83 2.668192430399465 1103 84-85 2.5311631510850248 1103 86-87 2.8823132271374483 1103 88-89 2.7406242412101776 1103 90-91 1.4636359842414493 1103 92-93 3.322474866713147 1103 94-95 2.7333860800657668 1103 96-97 3.4550735722610746 1103 98-99 3.9153399551837005 1103 100 3.2215902370199245 1104 1 0.8942502159299082 1104 2 -0.4583532786657756 1104 3 1.205193569451378 1104 4 0.7440726591117226 1104 5 0.4548308462371011 1104 6 -0.2812174370768119 1104 7 0.00586590059246106 1104 8 0.89421156100844 1104 9 0.8919631150099931 1104 10-11 1.285024762563836 1104 12-13 0.5389903065684436 1104 14-15 0.7158002696088559 1104 16-17 1.3703905252733364 1104 18-19 1.1850913192710095 1104 20-21 1.2849023264062254 1104 22-23 1.1443599911373425 1104 24-25 1.8991766480047687 1104 26-27 1.9673414259351887 1104 28-29 1.8795599474310478 1104 30-31 2.089037980248918 1104 32-33 2.3538934372918874 1104 34-35 -0.12354066289613286 1104 36-37 -0.8544024877032612 1104 38-39 0.05104064772032757 1104 40-41 0.4763624288038315 1104 42-43 -0.7257357551302874 1104 44-45 -0.1648937542492277 1104 46-47 0.15638292913683216 1104 48-49 1.114318613244393 1104 50-51 0.08388212265166572 1104 52-53 -0.057179690968752084 1104 54-55 0.53254501574812 1104 56-57 0.2703435019841365 1104 58-59 -0.36819124885531096 1104 60-61 -0.17918010808634932 1104 62-63 0.9364867337523677 1104 64-65 -0.029428340756460614 1104 66-67 1.4459085510843366 1104 68-69 1.32890175146035 1104 70-71 1.8772521098693034 1104 72-73 0.7907994782994763 1104 74-75 0.16721061056998465 1104 76-77 1.402041513174325 1104 78-79 0.8401942241786102 1104 80-81 -0.10936259920635649 1104 82-83 0.29914481135185156 1104 84-85 1.37402029394217 1104 86-87 1.7989798938041197 1104 88-89 2.0263385269244623 1104 90-91 2.616016936622401 1104 92-93 2.665332009570296 1104 94-95 2.3381479848276676 1104 96-97 3.233645000832503 1104 98-99 4.110578050421797 1104 100 4.259685475115159 1105 1 0.46567878735847756 1105 2 0.4702181499056515 1105 3 0.705193569451378 1105 4 0.4583583733974379 1105 5 0.9548308462371011 1105 6 0.861639705780334 1105 7 0.8630087577353223 1105 8 1.3227829895798635 1105 9 1.3919631150099931 1105 10-11 1.3564533339924054 1105 12-13 1.2889903065684365 1105 14-15 0.8586574124660018 1105 16-17 1.0132476681304894 1105 18-19 0.9708056049852942 1105 20-21 1.7849023264062254 1105 22-23 0.572931419708766 1105 24-25 1.00631950514763 1105 26-27 1.7173414259351887 1105 28-29 1.236702804573902 1105 30-31 1.4818951231060637 1105 32-33 -0.07467799127954322 1105 34-35 -0.5163978057532717 1105 36-37 1.0384546551538918 1105 38-39 -0.73467363799395 1105 40-41 -1.345066142624738 1105 42-43 -0.44002146941600273 1105 44-45 0.1922491028936335 1105 46-47 -1.8079027851488902 1105 48-49 -3.1713956724698917 1105 50-51 -3.0232607344911955 1105 52-53 -1.4143225481116062 1105 54-55 -1.9317406985375882 1105 56-57 -1.6582279265872941 1105 58-59 -1.868191248855311 1105 60-61 -0.7148943938006411 1105 62-63 -0.6706561233904935 1105 64-65 -2.743714055042176 1105 66-67 -1.6612343060585175 1105 68-69 -1.7068125342539382 1105 70-71 -0.44417646155927315 1105 72-73 0.040799478299472725 1105 74-75 -0.9042179608585883 1105 76-77 -0.3122442011113904 1105 78-79 -0.3383772043928204 1105 80-81 0.24778025793650116 1105 82-83 -0.41514090293386374 1105 84-85 -0.447408277486403 1105 86-87 0.6918370366612656 1105 88-89 -0.3665186159326801 1105 90-91 0.43744550805097404 1105 92-93 0.7010462952845806 1105 94-95 0.4452908419705288 1105 96-97 0.7693592865467913 1105 98-99 -0.7108505210067797 1105 100 -1.3117430963134105 1106 1 0.8823454540251419 1106 2 0.9821229118104178 1106 3 0.7170983313561443 1106 4 -0.017832102793043703 1106 5 1.0024498938561521 1106 6 1.064020658161283 1106 7 1.1368182815448407 1106 8 0.6680210848179584 1106 9 0.8443440673909421 1106 10-11 1.0231200006590768 1106 12-13 1.2949426875208232 1106 14-15 1.3467526505612355 1106 16-17 1.8108667157495262 1106 18-19 2.036281795461484 1106 20-21 1.5468070883109917 1106 22-23 1.37055046732781 1106 24-25 1.3694147432428707 1106 26-27 1.8721033306970938 1106 28-29 1.1355123283834274 1106 30-31 0.9342760754870199 1106 32-33 1.5741315325299894 1106 34-35 0.6264593371038671 1106 36-37 -1.0389262972270714 1106 38-39 -0.13348316180347553 1106 40-41 -2.0236375711961685 1106 42-43 -3.5650214694160027 1106 44-45 -4.123227087582563 1106 46-47 -2.420998023244124 1106 48-49 -1.3321099581841764 1106 50-51 -1.9756416868721445 1106 52-53 -1.58694159573065 1106 54-55 -1.6519787937756831 1106 56-57 -1.6998945932539584 1106 58-59 -1.4634293440934094 1106 60-61 -3.98275153665778 1106 62-63 -5.5456561233904935 1106 64-65 -4.91038072170884 1106 66-67 -4.917186687010897 1106 68-69 -4.558003010444416 1106 70-71 -3.991795509178324 1106 72-73 -4.483010045510053 1106 74-75 -3.9042179608585883 1106 76-77 -3.1098632487304414 1106 78-79 -3.4574248234404408 1106 80-81 -3.5260292658730243 1106 82-83 -3.4151409029338637 1106 84-85 -3.1021701822483045 1106 86-87 -2.7010201061958803 1106 88-89 -2.467709092123158 1106 90-91 -3.2946973490918836 1106 92-93 -3.769191799953518 1106 94-95 -3.1499472532675696 1106 96-97 -2.944926427738924 1106 98-99 -2.859660044816298 1106 100 -2.9784097629800783 1107 1 0.8293151509948444 1107 2 0.580607760295262 1107 3 1.0428559071137151 1107 4 0.7700466850857453 1107 5 0.7600256514319099 1107 6 0.6473539914946187 1107 7 0.08378797851454323 1107 8 0.933172599969474 1107 9 2.0867683098151844 1107 10-11 1.2428169703560457 1107 12-13 1.889639657217785 1107 14-15 1.6573587111672978 1107 16-17 -0.34064843576561543 1107 18-19 0.1006757348554217 1107 20-21 0.7362010277049293 1107 22-23 0.17358077035812158 1107 24-25 0.9186571674852928 1107 26-27 0.6751336337273983 1107 28-29 0.1847547526258566 1107 30-31 1.4039730451839887 1107 32-33 1.782464865863318 1107 34-35 1.5355502461947736 1107 36-37 1.6163767330759597 1107 38-39 2.044547141226829 1107 40-41 2.499089701531105 1107 42-43 2.8327058033112706 1107 44-45 3.562378973023499 1107 46-47 4.325214097967994 1107 48-49 4.792890041815824 1107 50-51 4.44102497979452 1107 52-53 4.485028101239038 1107 54-55 4.503324236527341 1107 56-57 4.773590255230893 1107 58-59 5.589600958936899 1107 60-61 5.051339372433127 1107 62-63 5.60207114933678 1107 64-65 6.0290132176850975 1107 66-67 6.169934525110314 1107 68-69 5.1503303228889195 1107 70-71 5.455174187791371 1107 72-73 5.365474802974802 1107 74-75 5.504872948232318 1107 76-77 5.450742811875621 1107 78-79 4.784999418983801 1107 80-81 4.1406374007936435 1107 82-83 4.698495460702496 1107 84-85 5.503890423812301 1107 86-87 4.68534353016776 1107 88-89 5.331533332119271 1107 90-91 4.966666287271757 1107 92-93 4.84065668489497 1107 94-95 5.312173958853645 1107 96-97 5.9641644813519825 1107 98-99 6.318370258214006 1107 100 5.870075085504769 1108 1 1.696448018127711 1108 2 1.1680203477078486 1108 3 1.287611151868962 1108 4 0.7001166151556788 1108 5 1.2075780989843565 1108 6 1.4935078376484654 1108 7 1.4893823841089429 1108 8 1.5975082643051408 1108 9 2.0238312468781245 1108 10-11 1.5679917955308724 1108 12-13 1.7917375593156848 1108 14-15 1.9685475223561042 1108 16-17 1.4775333824161976 1108 18-19 1.674102308281988 1108 20-21 2.2327045242084296 1108 22-23 2.421832518609868 1108 24-25 2.596978845806966 1108 26-27 2.8464623050560647 1108 28-29 2.47846104633215 1108 30-31 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-6.813020020344233 2216 68-69 -6.7871696771110805 2216 70-71 -6.0334621758449885 2216 72-73 -7.003843378843385 2216 74-75 -4.841717960858588 2216 76-77 -5.651529915397106 2216 78-79 -6.249091490107105 2216 80-81 -4.9843625992063565 2216 82-83 -4.977640902933864 2216 84-85 -4.581336848914972 2216 86-87 -4.76352010619588 2216 88-89 -5.509375758789822 2216 90-91 -4.357197349091884 2216 92-93 -7.60252513328685 2216 94-95 -7.045780586600902 2216 96-97 -6.632426427738924 2216 98-99 -6.755493378149634 2216 100 -6.9367430963134105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 7.0 7 9.0 8 19.0 9 29.0 10 26.0 11 25.0 12 32.0 13 64.0 14 60.0 15 49.0 16 63.0 17 72.0 18 88.0 19 48.0 20 38.0 21 38.0 22 44.0 23 59.0 24 56.0 25 68.0 26 97.0 27 100.0 28 122.0 29 137.0 30 174.0 31 193.0 32 254.0 33 315.0 34 394.0 35 575.0 36 904.0 37 1337.0 38 1500.0 39 603.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.92882515458493 21.036705696618867 13.327193790290751 24.70727535850546 2 13.652505589898725 22.75417598316454 38.590030251216625 25.00328817572011 3 15.862159673812966 29.330527423385504 31.250822043930025 23.5564908588715 4 10.798369064842825 19.05826647376036 36.55136130474813 33.592003156648694 5 12.166250164408785 38.62948835985795 32.81599368670262 16.388267789030646 6 27.712744969091148 38.5505721425753 18.768907010390635 14.967775877942918 7 25.016440878600555 34.11811127186637 22.806786794686307 18.05866105484677 8 24.687623306589504 34.27594370643167 21.294225963435487 19.742207023543337 9 26.213336840720768 17.821912402998816 21.833486781533608 34.13126397474681 10-11 22.510850979876366 27.50230172300408 28.40326187031435 21.583585426805207 12-13 24.470603709062214 26.430356438248058 28.370380113113246 20.728659739576482 14-15 23.069840852295144 26.930159147704853 27.127449690911483 22.872550309088517 16-17 19.900039458108644 30.349861896619757 27.64698145468894 22.103117190582662 18-19 20.37353676180455 28.054715243982635 30.974615283440748 20.597132710772065 20-21 22.293831382349072 26.930159147704853 30.895699066158095 19.88031040378798 22-23 21.38629488359858 27.101144285150596 31.19821123240826 20.31434959884256 24-25 21.04432460870709 26.890701039063526 31.467841641457316 20.597132710772065 26-27 20.952255688543996 27.60094699460739 30.461659871103514 20.9851374457451 28-29 20.31434959884256 27.916611863738 30.816782848875445 20.952255688543996 30-31 20.991713797185323 27.245824016835456 30.514270682625277 21.24819150335394 32-33 20.56425095357096 27.04195712218861 31.02722609496251 21.366565829277917 34-35 20.952255688543996 26.417203735367618 32.13205313691964 20.49848743916875 36-37 21.98474286465869 26.943311850585296 30.422201762462187 20.649743522293832 38-39 21.971590161778245 27.32474023411811 29.777719321320532 20.925950282783113 40-41 21.688807049848744 26.673681441536235 30.59976325134815 21.03774825726687 42-43 21.024595554386426 27.04195712218861 30.356438248059973 21.577009075364987 44-45 21.629619886886754 26.851242930422202 29.9881625674076 21.53097461528344 46-47 22.62922530580034 27.160331448112586 28.850453768249373 21.359989477837697 48-49 21.577009075364987 26.831513876101535 29.238458503222414 22.353018545311063 50-51 21.820334078653165 27.712744969091148 28.219124029988162 22.247796922267526 52-53 21.018019202946206 27.778508483493358 28.72550309088518 22.47796922267526 54-55 21.99131921609891 28.015257135341315 28.515059844798106 21.478363803761674 56-57 21.846639484414045 27.633828751808498 28.52821254767855 21.99131921609891 58-59 21.491516506642114 27.9494936209391 28.159936867026175 22.39905300539261 60-61 20.9851374457451 28.929369985532027 27.916611863738 22.168880704984876 62-63 21.577009075364987 27.706168617650928 28.857030119689597 21.859792187294488 64-65 20.71550703669604 27.712744969091148 28.646586873602526 22.925161120610284 66-67 21.215309746152837 28.284887544390376 28.47560173615678 22.02420097330001 68-69 21.715112455609628 27.64698145468894 28.350651058792582 22.287255030908852 70-71 21.767723267131394 27.055109825069053 28.061291595422862 23.115875312376694 72-73 21.91240299881626 27.87715375509667 27.706168617650928 22.504274628436143 74-75 21.438905695120347 27.44969091148231 28.205971327107722 22.90543206628962 76-77 20.83388136262002 28.199394975667502 28.738655793765616 22.228067867946862 78-79 22.076811784821782 28.02840983822176 27.45626726292253 22.438511114033936 80-81 21.550703669604104 27.57464158884651 28.705774036564513 22.168880704984876 82-83 21.511245560962777 27.226094962514797 28.429567276075236 22.83309220044719 84-85 21.794028672892278 27.548336183085624 28.087597001183745 22.570038142838353 86-87 21.80718137577272 27.048533473628833 28.19281862422728 22.95146652637117 88-89 21.432329343680127 27.653557806129157 27.52860712876496 23.385505721425755 90-91 21.471787452321454 27.462843614362754 28.232276732868606 22.83309220044719 92-93 20.860186768380903 27.101144285150596 28.791266605287387 23.24740234118111 94-95 21.169275286071287 27.403656451400764 28.561094304879653 22.865973957648297 96-97 21.6756543469683 26.71971590161778 28.238853084308822 23.36577666710509 98-99 21.774299618571614 27.548336183085624 27.870577403656448 22.806786794686307 100 21.544127318163884 27.673286860449824 26.857819281862422 23.924766539523873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 33.0 1 25.5 2 15.5 3 15.0 4 11.0 5 6.5 6 7.0 7 11.5 8 23.0 9 24.5 10 15.5 11 8.5 12 8.5 13 11.0 14 10.5 15 9.0 16 10.0 17 14.0 18 18.0 19 17.5 20 16.5 21 21.5 22 28.0 23 40.5 24 43.0 25 51.5 26 67.0 27 81.0 28 95.5 29 120.5 30 145.0 31 150.0 32 144.0 33 156.5 34 184.0 35 198.0 36 184.0 37 191.0 38 218.5 39 219.0 40 220.5 41 225.0 42 220.5 43 230.5 44 224.0 45 203.5 46 214.5 47 223.0 48 253.5 49 253.0 50 223.5 51 206.0 52 188.0 53 189.5 54 206.0 55 195.5 56 162.5 57 153.0 58 154.0 59 144.5 60 131.5 61 135.0 62 118.5 63 99.5 64 93.5 65 85.5 66 67.0 67 38.5 68 29.0 69 22.5 70 16.0 71 12.0 72 8.0 73 4.0 74 3.5 75 3.0 76 1.5 77 3.5 78 3.5 79 0.5 80 0.5 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026305405760883863 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7603.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.75364987504932 #Duplication Level Percentage of deduplicated Percentage of total 1 96.28985507246377 87.38655793765618 2 2.1594202898550723 3.9195054583716957 3 0.7391304347826086 2.0123635407076157 4 0.2753623188405797 0.9996054189135868 5 0.14492753623188406 0.6576351440220966 6 0.10144927536231885 0.5524135209785611 7 0.043478260869565216 0.27620676048928056 8 0.014492753623188406 0.10522162304353545 9 0.014492753623188406 0.11837432592397738 >10 0.2028985507246377 2.9067473365776664 >50 0.014492753623188406 1.0653689333157963 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81 1.0653689333157963 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49 0.6444824411416545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.381428383532816 No Hit TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT 15 0.19729054320662895 No Hit CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC 14 0.18413784032618705 No Hit CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT 14 0.18413784032618705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.1709851374457451 No Hit ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGG 13 0.1709851374457451 No Hit ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAA 12 0.15783243456530316 No Hit CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG 11 0.14467973168486123 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.14467973168486123 No Hit CCCACAGGCCGGTGGCATCGGCGCGGAGTGCCCAGGTTCACAGTGCAGCT 10 0.1315270288044193 No Hit GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC 10 0.1315270288044193 No Hit CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG 10 0.1315270288044193 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 10 0.1315270288044193 RNA PCR Primer, Index 27 (96% over 30bp) CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC 9 0.11837432592397738 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.10522162304353545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.013152702880441932 0.0 7 0.0 0.0 0.0 0.013152702880441932 0.0 8 0.0 0.0 0.0 0.013152702880441932 0.0 9 0.0 0.0 0.0 0.013152702880441932 0.0 10-11 0.0 0.0 0.0 0.013152702880441932 0.0 12-13 0.0 0.0 0.0 0.013152702880441932 0.0 14-15 0.0 0.0 0.0 0.013152702880441932 0.0 16-17 0.0 0.0 0.0 0.013152702880441932 0.0 18-19 0.0 0.0 0.0 0.013152702880441932 0.0 20-21 0.0 0.0 0.0 0.013152702880441932 0.0 22-23 0.0 0.0 0.0 0.013152702880441932 0.0 24-25 0.0 0.0 0.0 0.013152702880441932 0.0 26-27 0.0 0.0 0.0 0.013152702880441932 0.0 28-29 0.0 0.0 0.0 0.026305405760883863 0.0 30-31 0.0 0.0 0.0 0.032881757201104825 0.0 32-33 0.0 0.0 0.0 0.03945810864132579 0.0 34-35 0.0 0.0 0.0 0.046034460081546755 0.0 36-37 0.0 0.0 0.0 0.07891621728265158 0.0 38-39 0.0 0.0 0.0 0.11837432592397737 0.0 40-41 0.0 0.0 0.0 0.17756148888596607 0.0 42-43 0.0 0.0 0.0 0.21701959752729189 0.0 44-45 0.0 0.0 0.0 0.26963040904905955 0.0 46-47 0.0 0.0 0.0 0.4143101407339208 0.0 48-49 0.0 0.0 0.0 0.4866500065763515 0.0 50-51 0.0 0.0 0.0 0.5129554123372353 0.0 52-53 0.0 0.0 0.0 0.6050243325003288 0.0 54-55 0.0 0.0 0.0 0.7168223069840852 0.0 56-57 0.0 0.0 0.0 0.7891621728265159 0.0 58-59 0.0 0.0 0.0 0.8286202814678416 0.0 60-61 0.0 0.0 0.0 0.9141128501907142 0.0 62-63 0.0 0.0 0.0 1.0061817703538076 0.0 64-65 0.0 0.0 0.0 1.1837432592397739 0.0 66-67 0.0 0.0 0.0 1.3415756938050771 0.0 68-69 0.0 0.0 0.0 1.4862554254899383 0.0 70-71 0.0 0.0 0.0 1.5914770485334735 0.0 72-73 0.0 0.0 0.0 1.70327502301723 0.0 74-75 0.0 0.0 0.0 1.8808365119031962 0.0 76-77 0.0 0.0 0.0 2.078127055109825 0.0 78-79 0.0 0.0 0.0 2.2293831382349074 0.0 80-81 0.0 0.0 0.0 2.426673681441536 0.0 82-83 0.0 0.0 0.0 2.696304090490596 0.0 84-85 0.0 0.0 0.0 2.992239905300539 0.0 86-87 0.0 0.0 0.0 3.196106799947389 0.0 88 0.0 0.0 0.0 3.40655004603446 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTTCA 25 0.0050387145 56.399998 6 GTATCAA 40 5.3263E-4 46.999996 1 TCAACGC 40 5.3263E-4 46.999996 4 TATCAAC 40 5.3263E-4 46.999996 2 ATCAACG 40 5.3263E-4 46.999996 3 CAACGCA 40 5.3263E-4 46.999996 5 AACGCAG 45 9.516354E-4 41.77778 6 ACGCAGA 45 9.516354E-4 41.77778 7 CGCAGAG 50 0.0015978694 37.6 8 GCAGAGT 60 0.003908552 31.333334 9 GAGTACT 60 1.7246399E-4 23.5 12-13 CAGAGTA 75 7.788353E-4 18.800001 10-11 AGTACTT 65 0.0076891403 18.076923 12-13 AGAGTAC 65 0.0076891403 18.076923 10-11 >>END_MODULE