FastQCFastQC Report
Wed 25 May 2016
SRR2431410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193036
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA253790.794823484608379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40150.049.2379951
GTATCAA70350.038.8730551
ATCAACG81750.032.411263
TCAACGC81950.032.0420044
TATCAAC88800.030.0510082
CAACGCA89300.029.2988745
AACGCAG95100.027.6235756
GTACATG153400.027.4928191
TACATGG155200.026.3522052
CATGGGT75150.026.1746964
ACATGGG159650.024.9093883
CGCAGAG105950.024.4391238
ACGCAGA108050.024.1392427
ATGGGTA33800.021.8580045
ATGGGTG43050.021.7524175
GCAGAGT115900.021.6111959
GGGTACG1708.640012E-519.3510517
GTGGTAT20200.019.3403051
TGGTATC19100.018.727042
AGAGTAC105500.018.46320510-11
Produced by FastQC (version 0.11.5)