##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431410_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3193036
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54920520783355	33.0	31.0	34.0	26.0	34.0
2	30.559490090309033	33.0	31.0	34.0	26.0	34.0
3	30.733718316987343	33.0	31.0	34.0	26.0	34.0
4	33.95938129103462	37.0	35.0	37.0	28.0	37.0
5	34.04217678723322	37.0	35.0	37.0	30.0	37.0
6	34.08322831311642	37.0	35.0	37.0	30.0	37.0
7	33.924699564928176	37.0	35.0	37.0	30.0	37.0
8	33.92794224681463	37.0	35.0	37.0	30.0	37.0
9	35.33007081661466	39.0	37.0	39.0	28.0	39.0
10-11	35.37830171661077	39.0	37.0	39.0	28.5	39.0
12-13	35.32217269081839	39.0	37.0	39.0	27.5	39.0
14-15	36.62489430122304	40.0	38.0	41.0	27.5	41.0
16-17	36.63336586245818	40.0	38.0	41.0	28.0	41.0
18-19	36.64023957136719	40.0	38.0	41.0	28.0	41.0
20-21	36.74301886981544	40.0	38.0	41.0	29.0	41.0
22-23	36.70790072520322	40.0	38.0	41.0	28.0	41.0
24-25	36.76081290658796	40.0	38.0	41.0	29.0	41.0
26-27	36.81666351397229	40.0	38.0	41.0	29.5	41.0
28-29	36.782354004151536	40.0	37.0	41.0	30.0	41.0
30-31	36.71136874122308	40.0	37.0	41.0	30.0	41.0
32-33	36.66348234094448	40.0	37.0	41.0	30.0	41.0
34-35	36.58066116385784	40.0	37.0	41.0	29.5	41.0
36-37	36.42985234115744	40.0	37.0	41.0	29.0	41.0
38-39	36.29499322901464	40.0	37.0	41.0	28.0	41.0
40-41	36.231930520044244	40.0	36.0	41.0	28.0	41.0
42-43	36.038672285561454	40.0	36.0	41.0	27.0	41.0
44-45	35.85864879068072	40.0	35.5	41.0	27.0	41.0
46-47	35.712034878404125	40.0	35.0	41.0	27.0	41.0
48-49	35.56592362253354	40.0	35.0	41.0	27.0	41.0
50-51	35.42230153371274	39.5	35.0	41.0	26.5	41.0
52-53	35.25594997989374	39.0	35.0	41.0	26.0	41.0
54-55	35.08822355902032	39.0	35.0	41.0	26.0	41.0
56-57	34.88700675469992	39.0	34.0	41.0	26.0	41.0
58-59	34.66411449792611	38.5	34.0	40.5	25.5	41.0
60-61	34.41908406294198	38.0	34.0	40.0	24.5	41.0
62-63	34.3239717622977	38.0	34.0	40.0	24.5	41.0
64-65	34.08025105260323	38.0	33.5	40.0	23.5	41.0
66-67	33.82555943622308	37.0	33.0	40.0	23.0	41.0
68-69	33.53345702961069	37.0	33.0	40.0	22.5	41.0
70-71	33.20596166156598	36.0	33.0	39.5	22.0	41.0
72-73	32.86663084913543	36.0	32.5	39.0	21.5	41.0
74-75	32.581403090976735	35.0	32.0	39.0	20.0	40.5
76-77	32.32275693102113	35.0	32.0	38.5	20.0	40.0
78-79	31.972395081045125	35.0	32.0	37.0	18.5	39.5
80-81	31.640211385026667	35.0	32.0	37.0	18.0	39.0
82-83	31.300605129412887	35.0	32.0	36.5	15.0	39.0
84-85	30.99274373981377	35.0	31.0	36.0	11.0	37.5
86-87	30.700369178424545	35.0	31.0	36.0	7.0	37.0
88-89	30.424054567502527	35.0	31.0	35.0	3.5	37.0
90-91	30.18482691707829	35.0	31.0	35.0	2.0	36.0
92-93	29.942727078554704	35.0	31.0	35.0	2.0	36.0
94-95	29.721893051002244	34.0	31.0	35.0	2.0	36.0
96-97	29.507399227568996	34.0	30.0	35.0	2.0	36.0
98-99	29.281011081616366	34.0	30.0	35.0	2.0	35.0
100	29.09769542216248	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3595445221110367
1101	2	0.33871678466804767
1101	3	0.3345936559655449
1101	4	0.4803240887996125
1101	5	0.30674295632906734
1101	6	0.3794523149726885
1101	7	0.35726993855382716
1101	8	0.18658568821653176
1101	9	0.28660333516798886
1101	10-11	0.30525341676037243
1101	12-13	0.1980628386538683
1101	14-15	0.345811831150165
1101	16-17	0.3483745190264571
1101	18-19	0.32520907981038505
1101	20-21	0.3769709456462138
1101	22-23	0.38716584878454796
1101	24-25	0.3654197369663379
1101	26-27	0.35970515128062175
1101	28-29	0.3697778709785595
1101	30-31	0.4672059839263696
1101	32-33	0.449538684035538
1101	34-35	0.4730839247183596
1101	36-37	0.4018729844642408
1101	38-39	0.46646766995186084
1101	40-41	0.38029735496761674
1101	42-43	0.4448555977490827
1101	44-45	0.40041695957685874
1101	46-47	0.37676231229649204
1101	48-49	0.38126567467860895
1101	50-51	0.37521708010155663
1101	52-53	0.29364687047470994
1101	54-55	0.40940313202870726
1101	56-57	0.2642703626756955
1101	58-59	0.39262597126131027
1101	60-61	0.3999647898584797
1101	62-63	0.3365070089448139
1101	64-65	0.3434348141923209
1101	66-67	0.3957172830023339
1101	68-69	0.34841004996480507
1101	70-71	0.41820744606472005
1101	72-73	0.35201503250507926
1101	74-75	0.4199131164320278
1101	76-77	0.3825805714577797
1101	78-79	0.3940708608888279
1101	80-81	0.35019546459817974
1101	82-83	0.34055855528859524
1101	84-85	0.2980109119801533
1101	86-87	0.30142558126993535
1101	88-89	0.3068894023183226
1101	90-91	0.2591895936479496
1101	92-93	0.3148643316238342
1101	94-95	0.28924269510198997
1101	96-97	0.3257848932625542
1101	98-99	0.2906983065602695
1101	100	0.3378530329549818
1102	1	0.21473384484749758
1102	2	0.2260190202241681
1102	3	0.24123226534954867
1102	4	0.3931071129194095
1102	5	0.33463188909781394
1102	6	0.252775775725226
1102	7	0.23187052788693308
1102	8	0.3657805142465804
1102	9	0.40539265706766514
1102	10-11	0.37457473997752544
1102	12-13	0.3088314983313154
1102	14-15	0.4226956039419676
1102	16-17	0.35466365206711714
1102	18-19	0.33689651244582564
1102	20-21	0.39011165130717274
1102	22-23	0.2993344593237879
1102	24-25	0.3607964682805189
1102	26-27	0.3083237376575312
1102	28-29	0.26600506058673545
1102	30-31	0.30967288013712846
1102	32-33	0.2920351003630941
1102	34-35	0.2993101310974495
1102	36-37	0.29840655569407204
1102	38-39	0.28346737544801215
1102	40-41	0.36093192590666945
1102	42-43	0.31241110660965177
1102	44-45	0.26358308275570863
1102	46-47	0.15152258919054162
1102	48-49	0.17069221967901171
1102	50-51	0.1412389142956556
1102	52-53	0.14486213465302455
1102	54-55	0.14221102277917197
1102	56-57	0.14757348244067003
1102	58-59	0.09240612774581791
1102	60-61	0.15208780925041054
1102	62-63	0.1694831178726517
1102	64-65	0.20870967321052092
1102	66-67	0.1866620685293796
1102	68-69	0.11597394997668431
1102	70-71	0.05656216676140957
1102	72-73	0.04929550917826475
1102	74-75	0.09217252806122644
1102	76-77	0.057532194494719135
1102	78-79	0.03489650833820335
1102	80-81	-0.0062641994164032155
1102	82-83	0.023281221229421334
1102	84-85	0.008984445917015194
1102	86-87	-0.0466205007945959
1102	88-89	-0.06679750453362132
1102	90-91	-0.03097569808634759
1102	92-93	-0.021905530707822862
1102	94-95	-0.0032504321731749997
1102	96-97	-0.01677604892659801
1102	98-99	-0.05833293385876814
1102	100	-0.0610401718627962
1103	1	-0.1953364274909255
1103	2	-0.1671399594528964
1103	3	-0.11945060662312912
1103	4	-0.1020313183123136
1103	5	-0.08099931230878354
1103	6	-0.13574574098895908
1103	7	-0.1572110114750913
1103	8	-0.19616222440514264
1103	9	-0.1570992778644893
1103	10-11	-0.16714498932643806
1103	12-13	-0.14593824210997752
1103	14-15	-0.14526595001492382
1103	16-17	-0.10898322591949494
1103	18-19	-0.15385634162648643
1103	20-21	-0.26035456201879725
1103	22-23	-0.22856320063750957
1103	24-25	-0.08195001528080326
1103	26-27	-0.1146964595067459
1103	28-29	-0.1404883181681953
1103	30-31	-0.18640818217369315
1103	32-33	-0.13906575922744224
1103	34-35	-0.08170606399645663
1103	36-37	-0.2232340628093823
1103	38-39	-0.2328825815805189
1103	40-41	-0.14342591732431487
1103	42-43	-0.184228319841651
1103	44-45	-0.2937864140232165
1103	46-47	-0.2825116861672896
1103	48-49	-0.32639044286104735
1103	50-51	-0.4768225762463558
1103	52-53	-0.45768655347917786
1103	54-55	-0.4591851870247794
1103	56-57	-0.4198285325996878
1103	58-59	-0.3029561559082339
1103	60-61	-0.29281332873902244
1103	62-63	-0.2326093194950829
1103	64-65	-0.34659125027862103
1103	66-67	-0.2845212971947504
1103	68-69	-0.3091165480891931
1103	70-71	-0.3151204327092927
1103	72-73	-0.25220147798189885
1103	74-75	-0.2602635082628453
1103	76-77	-0.2503318945136783
1103	78-79	-0.19822756897311322
1103	80-81	-0.22685307087816753
1103	82-83	-0.1880893208698282
1103	84-85	-0.2808550313030409
1103	86-87	-0.34358639981038763
1103	88-89	-0.3532859906918837
1103	90-91	-0.37833999590543144
1103	92-93	-0.4571069806118011
1103	94-95	-0.3654187278242276
1103	96-97	-0.3161275955883589
1103	98-99	-0.47624734704777083
1103	100	-0.41431112842763795
1104	1	-0.0729951117453993
1104	2	-0.03633685440897594
1104	3	0.03881443392769768
1104	4	-0.03446784585340623
1104	5	-0.01873866660047696
1104	6	0.04495636267355252
1104	7	-0.02471139055511884
1104	8	0.007864046123088997
1104	9	0.006999866420258627
1104	10-11	0.008096373354966602
1104	12-13	0.07642325357370083
1104	14-15	-0.0028161281203935573
1104	16-17	0.04932420832000872
1104	18-19	0.06499845479228128
1104	20-21	0.0053604461831184835
1104	22-23	0.04201594098972805
1104	24-25	0.05825651158462364
1104	26-27	0.0014247349196097048
1104	28-29	0.016187456211504525
1104	30-31	0.013878901739374783
1104	32-33	-0.03186104674862378
1104	34-35	0.04930837674921662
1104	36-37	-0.06818771109588795
1104	38-39	-7.946494773065638E-4
1104	40-41	0.018628025254841418
1104	42-43	-0.02338099293773155
1104	44-45	-0.03523822159654344
1104	46-47	-0.1296461236498203
1104	48-49	-0.19492108348708115
1104	50-51	-0.24703468253700578
1104	52-53	-0.25815704207096246
1104	54-55	-0.09390455594635938
1104	56-57	-0.15007150312273865
1104	58-59	-0.26667420895321925
1104	60-61	-0.22851441888504098
1104	62-63	-0.14442976911676197
1104	64-65	-0.19184719087502344
1104	66-67	-0.08213464099242884
1104	68-69	0.005127550521784485
1104	70-71	-0.10576756242380725
1104	72-73	-0.07705434860635307
1104	74-75	-0.015594367727089775
1104	76-77	-0.06523709513006537
1104	78-79	-0.0701925729379198
1104	80-81	-0.053775571554407264
1104	82-83	-0.09599895271137626
1104	84-85	-0.028474284455505483
1104	86-87	0.05589059404320551
1104	88-89	-0.01960632924480521
1104	90-91	-0.03143341320056692
1104	92-93	-0.04595696593445453
1104	94-95	-0.06576804639539802
1104	96-97	-0.037873614457165417
1104	98-99	-0.09648208515036671
1104	100	4.1340185774885185E-4
1105	1	0.10104922556749685
1105	2	6.351891575242519E-4
1105	3	0.05355210148473688
1105	4	0.13569992839209988
1105	5	0.15493786394129927
1105	6	0.10666881396867467
1105	7	0.11156023842119112
1105	8	0.12499234630331557
1105	9	0.14232468814202548
1105	10-11	0.15805961495566834
1105	12-13	0.18975519083045356
1105	14-15	0.15508581383698328
1105	16-17	0.09200761602412655
1105	18-19	0.1839620015990917
1105	20-21	0.2321756984682679
1105	22-23	0.15402502142823948
1105	24-25	0.15897263203632406
1105	26-27	0.15075929650329556
1105	28-29	0.15913674967050895
1105	30-31	0.18015518677960074
1105	32-33	0.1681649069090625
1105	34-35	0.07038865040992448
1105	36-37	0.0726331596548988
1105	38-39	0.04415741979779142
1105	40-41	-0.021772797242185504
1105	42-43	-0.042563085136706036
1105	44-45	-0.031027045706494505
1105	46-47	-0.003594666346366182
1105	48-49	-0.1620306703003962
1105	50-51	-0.1282921316823149
1105	52-53	0.0050407827229150826
1105	54-55	-0.10915762081713609
1105	56-57	-0.18335066422923063
1105	58-59	-0.14066931228887825
1105	60-61	-0.13805623100729036
1105	62-63	-0.02926893441148337
1105	64-65	-0.09027593638882792
1105	66-67	-0.06591728078016246
1105	68-69	-0.030690279306881507
1105	70-71	0.04816636602429014
1105	72-73	0.01415983701689072
1105	74-75	0.010626735158552947
1105	76-77	-0.01398652983044002
1105	78-79	-0.08731062532197953
1105	80-81	-0.006447684404921716
1105	82-83	0.09144816531873445
1105	84-85	0.02067548262990826
1105	86-87	0.022251089234540444
1105	88-89	0.07048616825450793
1105	90-91	0.0795296123053042
1105	92-93	-0.02589144687243916
1105	94-95	-0.0384065420189188
1105	96-97	-0.007228471132822989
1105	98-99	-0.0780506440206139
1105	100	-0.04176652716512663
1106	1	-0.05312961178928788
1106	2	-0.13230500955836177
1106	3	-0.010364647091002155
1106	4	0.07314710680176262
1106	5	0.04379438440930983
1106	6	0.08694670042839192
1106	7	0.014956782711514904
1106	8	0.06130167543550158
1106	9	0.07475877122168129
1106	10-11	0.11219880629843004
1106	12-13	0.07342437303816496
1106	14-15	0.08866208383241059
1106	16-17	0.037244672294569625
1106	18-19	-0.04210026433109704
1106	20-21	-0.015052116897557255
1106	22-23	0.1022938699848055
1106	24-25	0.12216083088407714
1106	26-27	0.005685855949323582
1106	28-29	-0.06434011033047682
1106	30-31	-0.04089884197547633
1106	32-33	-0.04371080934346594
1106	34-35	0.02232779160959808
1106	36-37	-0.03331109648785002
1106	38-39	-0.047934631827040164
1106	40-41	-0.08222863097530819
1106	42-43	-0.040576490174089486
1106	44-45	-0.03447954553081445
1106	46-47	-0.10123438384466255
1106	48-49	-0.039077059606910325
1106	50-51	-0.13630622206655119
1106	52-53	-0.21001611407859855
1106	54-55	-0.1522213034199993
1106	56-57	-0.20903134811863566
1106	58-59	-0.24823497762620406
1106	60-61	-0.2795316873485305
1106	62-63	-0.2890363415708421
1106	64-65	-0.13624922000704487
1106	66-67	-0.04118055373646001
1106	68-69	-0.027597586579105382
1106	70-71	-0.10798839570489349
1106	72-73	-0.10530848130677839
1106	74-75	-0.14358411396884208
1106	76-77	-0.10753715570826472
1106	78-79	-0.03385114190000138
1106	80-81	-0.029915044978043426
1106	82-83	-0.06899318070248128
1106	84-85	-0.07289657118109716
1106	86-87	-0.09991823307888126
1106	88-89	-0.03905290562501307
1106	90-91	-0.06636446603764412
1106	92-93	-0.09013529675198484
1106	94-95	-0.0833739706929606
1106	96-97	-0.0780424983610466
1106	98-99	-0.011757867316159576
1106	100	-0.01883256597310634
1107	1	-0.05362451205536445
1107	2	-0.1441787762007678
1107	3	-0.192202225431366
1107	4	-0.032030590978514795
1107	5	-0.04475044071298839
1107	6	-0.0729696546004277
1107	7	-0.09601030957791323
1107	8	-0.12164422351798265
1107	9	-0.09150335714128488
1107	10-11	-0.1551097332141822
1107	12-13	-0.0738899653403422
1107	14-15	-0.09296066086228194
1107	16-17	-0.05087155213178107
1107	18-19	-0.12987428228898779
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2216	76-77	-0.6434157378567775
2216	78-79	-0.6032661822880634
2216	80-81	-0.6741408359103076
2216	82-83	-0.6497815495212755
2216	84-85	-0.64112193187173
2216	86-87	-0.7342967187231189
2216	88-89	-0.6658793397716671
2216	90-91	-0.6695801273382962
2216	92-93	-0.6001700430278838
2216	94-95	-0.7244013794853714
2216	96-97	-0.7336991096301553
2216	98-99	-0.8139763520380008
2216	100	-0.8915741528065766
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81361.0
3	16329.0
4	11306.0
5	10106.0
6	6837.0
7	6313.0
8	6967.0
9	7400.0
10	7695.0
11	8254.0
12	9488.0
13	10300.0
14	10922.0
15	11175.0
16	10953.0
17	10969.0
18	10772.0
19	10556.0
20	10187.0
21	10655.0
22	12679.0
23	19184.0
24	31162.0
25	39321.0
26	40996.0
27	32386.0
28	31256.0
29	36957.0
30	44242.0
31	51955.0
32	66736.0
33	88173.0
34	124691.0
35	198436.0
36	323229.0
37	572752.0
38	834449.0
39	374842.0
40	1045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15426537303328	21.551625966907505	14.108622413462621	22.18548624659659
2	14.99118342829591	22.651734908698838	39.76821247106499	22.588869191940265
3	17.301337151824885	28.523479299230665	30.652932563234614	23.522250985709835
4	11.75463759441142	19.430024180868376	39.05934916195159	29.755989062768617
5	11.974607266352933	40.71675831942096	34.49934754868755	12.809286865538555
6	27.92476873761262	40.47630837659383	18.538505704618476	13.06041718117507
7	24.947464127519016	35.69035662337491	22.726242737826485	16.63593651127958
8	23.779660607967447	37.76661603561702	20.857903285398358	17.595820071017176
9	24.697334285527166	19.13398827472944	21.294379873321454	34.874297566421944
10-11	22.03213183435335	29.77281189121698	29.05129823567315	19.143758038756523
12-13	22.919649810630098	27.992647715573778	28.457352206077076	20.630350267719052
14-15	20.981759726424883	29.40973229307349	26.10300402687803	23.505503953623602
16-17	20.097199935507152	32.21355092514176	26.788332936651134	20.900916202699957
18-19	20.147767805447046	31.059366141268978	28.683300821449347	20.109565231834623
20-21	22.078250738639525	29.982347866896802	27.884462753842453	20.054938640621216
22-23	21.64212326164483	29.931698199715033	28.003659769316698	20.422518769323435
24-25	21.207075642953587	30.000076780400377	27.98518671034688	20.807660866299155
26-27	20.94970198020159	29.951863342601094	28.065572033948104	21.032862643249217
28-29	20.8451808601422	30.004049025805017	28.242769809435668	20.90800030461711
30-31	21.184395130385074	30.36883789946574	27.84030443633786	20.606462533811328
32-33	20.72631434948227	30.151590106394664	28.288065548277185	20.834029995845878
34-35	21.02683877539028	29.87530605449239	28.189481519205533	20.908373650911795
36-37	21.25872144457102	30.001816339122268	27.863174970188687	20.876287246118025
38-39	21.091016394346916	30.13747832704196	28.008435372273404	20.76306990633772
40-41	20.899338448421247	29.824181193394107	28.261902780735543	21.01457757744911
42-43	20.83798103518254	29.839953990494084	28.2404654858187	21.081599488504676
44-45	21.12122001917995	29.892262837739462	28.112812216867294	20.873704926213296
46-47	21.080273312194397	30.09037066892912	27.999832008467273	20.829524010409205
48-49	20.97495269102834	29.826036193812026	28.09481383968625	21.104197275473382
50-51	20.93165207135569	29.773791876992878	28.10754284605665	21.18701320559478
52-53	20.997341888434534	29.831308172635275	28.02188985119151	21.14946008773868
54-55	21.112640281632046	30.13738087462146	27.8735990642354	20.876379779511094
56-57	21.09873437398633	29.811975417714876	27.820878018448937	21.268412189849855
58-59	20.951441859473807	29.543360626206017	28.15521504283555	21.349982471484623
60-61	21.156082571192478	29.91189666863441	27.800886251721252	21.13113450845186
62-63	20.99311699540851	29.856141035938727	27.95964026484039	21.191101703812368
64-65	21.029825959607134	29.630115979918585	28.04238483570478	21.297673224769504
66-67	20.83393140658681	29.710680888378533	27.942065975889445	21.51332172914521
68-69	21.30442072406358	29.85228212906784	27.755697487840347	21.087599659028232
70-71	21.183000913508167	29.65817871855017	27.846831474195355	21.31198889374631
72-73	20.995922373421845	29.724321481934595	28.114564635332577	21.165191509310976
74-75	20.993230926793753	29.813725522456842	27.960288438139656	21.232755112609745
76-77	21.080708040152654	29.80719416884512	27.77314107178843	21.338956719213794
78-79	21.18224848751614	29.765434747147363	27.954183518834498	21.098133246502
80-81	21.05310253039408	29.6798641145893	28.026506994936266	21.240526360080352
82-83	20.972566401775737	29.717822130514136	28.176665621395124	21.132945846314996
84-85	20.967670908456356	29.587497823628244	27.99979251008418	21.445038757831217
86-87	21.13546096123273	29.72579974096126	27.89817411383444	21.240565183971565
88-89	21.034208190916086	29.569401699463317	27.975608315124873	21.42078179449573
90-91	20.977978810777742	29.482423777236576	27.943778527855695	21.59581888412999
92-93	21.088075830013633	29.63806906865451	27.86132805001933	21.412527051312527
94-95	20.893347373171373	29.487357877978766	28.05162774372281	21.56766700512705
96-97	20.863204401467154	29.575240619053467	27.966151624476332	21.595403355003047
98-99	20.88652871818169	29.56710348142243	27.9160374163705	21.630330384025378
100	20.988850032895428	29.681235626860847	27.748977953637976	21.58093638660575
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23210.0
1	15378.5
2	6128.0
3	3885.0
4	2474.5
5	1765.0
6	1672.0
7	1826.0
8	2196.5
9	2290.0
10	1722.0
11	1212.5
12	1118.5
13	1151.0
14	1202.0
15	1354.5
16	1610.0
17	2008.0
18	2557.5
19	3312.0
20	4488.0
21	6198.5
22	8932.5
23	13376.0
24	18209.0
25	23378.5
26	30818.0
27	40158.5
28	50899.0
29	60977.5
30	68985.5
31	76826.0
32	84308.5
33	91256.0
34	97932.0
35	103508.0
36	106675.5
37	108802.0
38	110001.5
39	109915.5
40	110377.0
41	109256.0
42	105474.0
43	101465.0
44	98111.5
45	95340.5
46	93535.0
47	91396.5
48	89280.0
49	87937.5
50	86909.5
51	84384.5
52	80100.5
53	76115.5
54	73191.0
55	70797.5
56	65652.5
57	58776.5
58	53297.0
59	48530.0
60	42700.0
61	36270.0
62	30775.5
63	25439.5
64	20529.5
65	16485.5
66	13098.5
67	10292.5
68	7982.0
69	6263.5
70	4823.0
71	3621.5
72	2796.5
73	2155.0
74	1734.5
75	1371.5
76	1015.5
77	768.5
78	591.0
79	446.5
80	325.5
81	248.5
82	193.0
83	146.0
84	127.0
85	119.0
86	95.5
87	84.5
88	77.5
89	68.5
90	61.0
91	51.5
92	42.0
93	32.5
94	22.5
95	23.0
96	21.0
97	20.0
98	19.5
99	15.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16990099704481879
2	0.16564172781014683
3	0.17973489807192902
4	0.16886749789228808
5	0.1824282594997363
6	0.07165594124212818
7	0.07387953032787604
8	0.061915994683429815
9	0.05665454445236446
10-11	0.07027794237208726
12-13	0.06861808009681068
14-15	0.06584642327866018
16-17	0.06215088085445952
18-19	0.06738101292938758
20-21	0.06900955704852686
22-23	0.06653542271368065
24-25	0.0663161956207196
26-27	0.06966723832741002
28-29	0.06717744491449516
30-31	0.07367596231298362
32-33	0.06976119279582191
34-35	0.06963592017127272
36-37	0.07993959354044239
38-39	0.07591521047680014
40-41	0.07138973691496119
42-43	0.07488171132426945
44-45	0.08535763455219421
46-47	0.07513225657336779
48-49	0.08059727481932556
50-51	0.07693305055126218
52-53	0.07594652863293744
54-55	0.07798220878186152
56-57	0.07149935046144171
58-59	0.07893741254404898
60-61	0.07539846090053479
62-63	0.06680162704084765
64-65	0.06968289740547867
66-67	0.06996476081071432
68-69	0.0675219446320054
70-71	0.06392035667621661
72-73	0.06150885865364499
74-75	0.06659805902595524
76-77	0.07243889514556053
78-79	0.06813264867668263
80-81	0.0753671427443975
82-83	0.07759073183014535
84-85	0.0789060943879117
86-87	0.07496000671461267
88-89	0.07643196005306548
90-91	0.0753045064321229
92-93	0.06606565037162124
94-95	0.06626921838651366
96-97	0.06576812788831696
98-99	0.07517923380757374
100	0.0821475235481216
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3193036.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.84205139514197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17954930142369	54.50882602452787
2	13.744602360158813	18.924132441676853
3	3.7955798705974995	7.838865135781179
4	1.4366849531578634	3.9561735753568303
5	0.6073010102240046	2.0903923679081284
6	0.3512918626744946	1.4510191478959626
7	0.20209043653336795	0.9738624152807753
8	0.14212108521612732	0.7827125642225591
9	0.0906096406718415	0.5613978186023633
>10	0.41283389235621093	4.860810793458911
>50	0.023635228113216538	1.141289082079937
>100	0.012746541029616035	1.580003167551909
>500	6.358785634619824E-4	0.2654026913482991
>1k	2.725193826618131E-4	0.27156248370328057
>5k	0.0	0.0
>10k+	4.5419897110302176E-5	0.7935502906051304
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25379	0.794823484608379	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.131815613729378E-5	0.0
5	0.0	0.0	0.0	3.131815613729378E-5	0.0
6	0.0	0.0	0.0	3.131815613729378E-5	0.0
7	0.0	0.0	0.0	3.131815613729378E-5	0.0
8	0.0	0.0	0.0	6.263631227458756E-5	0.0
9	0.0	0.0	0.0	6.263631227458756E-5	0.0
10-11	3.131815613729378E-5	0.0	0.0	6.263631227458756E-5	0.0
12-13	3.131815613729378E-5	0.0	0.0	6.263631227458756E-5	0.0
14-15	3.131815613729378E-5	0.0	0.0	6.263631227458756E-5	0.0
16-17	3.131815613729378E-5	0.0	0.0	1.5659078068646894E-4	0.0
18-19	3.131815613729378E-5	0.0	0.0	1.8790893682376272E-4	0.0
20-21	3.131815613729378E-5	0.0	0.0	2.192270929610565E-4	0.0
22-23	3.131815613729378E-5	0.0	0.0	4.38454185922113E-4	0.0
24-25	3.131815613729378E-5	0.0	0.0	6.576812788831694E-4	0.0
26-27	3.131815613729378E-5	0.0	0.0	0.0011274536209425763	0.0
28-29	3.131815613729378E-5	0.0	0.0	0.0024897934129148556	0.0
30-31	3.131815613729378E-5	0.0	0.0	0.005872154275742584	0.0
32-33	3.131815613729378E-5	0.0	0.0	0.012495944298780221	0.0
34-35	3.131815613729378E-5	0.0	0.0	0.023363344478421165	0.0
36-37	3.131815613729378E-5	0.0	0.0	0.04088585283723704	0.0
38-39	3.131815613729378E-5	0.0	0.0	0.06490687859454138	0.0
40-41	3.131815613729378E-5	0.0	0.0	0.09144901592089785	0.0
42-43	3.131815613729378E-5	0.0	0.0	0.1159398140202616	0.0
44-45	3.131815613729378E-5	0.0	0.0	0.14797828774871313	0.0
46-47	3.131815613729378E-5	0.0	0.0	0.18725125554487954	0.0
48-49	3.131815613729378E-5	0.0	0.0	0.23040767470207038	0.0
50-51	3.131815613729378E-5	0.0	0.0	0.2751926379784005	0.0
52-53	3.131815613729378E-5	0.0	0.0	0.31878751132151345	0.0
54-55	3.131815613729378E-5	0.0	0.0	0.3674246078027307	0.0
56-57	3.131815613729378E-5	0.0	0.0	0.4274928312740601	0.0
58-59	3.131815613729378E-5	0.0	0.0	0.49986909010734615	0.0
60-61	3.131815613729378E-5	0.0	0.0	0.5759095732086954	0.0
62-63	3.131815613729378E-5	0.0	0.0	0.6531401462432619	0.0
64-65	3.131815613729378E-5	0.0	0.0	0.7374016453306509	0.0
66-67	3.131815613729378E-5	0.0	0.0	0.8381834717804623	0.0
68-69	3.131815613729378E-5	0.0	0.0	0.9578188282249245	0.0
70-71	3.131815613729378E-5	0.0	0.0	1.0911871961355901	0.0
72-73	3.131815613729378E-5	0.0	0.0	1.2292846056229871	0.0
74-75	3.131815613729378E-5	0.0	0.0	1.370482512567976	0.0
76-77	3.131815613729378E-5	0.0	0.0	1.5287018373735841	0.0
78-79	3.131815613729378E-5	0.0	0.0	1.7123828231188123	0.0
80-81	3.131815613729378E-5	0.0	0.0	1.9231070366885936	0.0
82-83	3.131815613729378E-5	0.0	0.0	2.1403297676568633	0.0
84-85	3.131815613729378E-5	0.0	0.0	2.3599796557257733	0.0
86-87	3.131815613729378E-5	0.0	0.0	2.598201210384098	0.0
88	3.131815613729378E-5	0.0	0.0	2.7938927090079786	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4015	0.0	49.237995	1
GTATCAA	7035	0.0	38.873055	1
ATCAACG	8175	0.0	32.41126	3
TCAACGC	8195	0.0	32.042004	4
TATCAAC	8880	0.0	30.051008	2
CAACGCA	8930	0.0	29.298874	5
AACGCAG	9510	0.0	27.623575	6
GTACATG	15340	0.0	27.492819	1
TACATGG	15520	0.0	26.352205	2
CATGGGT	7515	0.0	26.174696	4
ACATGGG	15965	0.0	24.909388	3
CGCAGAG	10595	0.0	24.439123	8
ACGCAGA	10805	0.0	24.139242	7
ATGGGTA	3380	0.0	21.858004	5
ATGGGTG	4305	0.0	21.752417	5
GCAGAGT	11590	0.0	21.611195	9
GGGTACG	170	8.640012E-5	19.351051	7
GTGGTAT	2020	0.0	19.340305	1
TGGTATC	1910	0.0	18.72704	2
AGAGTAC	10550	0.0	18.463205	10-11
>>END_MODULE