FastQCFastQC Report
Wed 25 May 2016
SRR2431410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193036
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242060.7580872874593334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199780.6256741233108553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127160.39824167344182776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46670.1461618346927501No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43740.13698561494452302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42080.13178680102573226No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.1269324868244517No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32480.10172137113393022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94150.064.992551
GTATCAA179950.043.6486971
GTGGTAT31350.036.1564751
TGGTATC31600.033.7834932
ATCAACG232250.033.6133163
TATCAAC233550.033.487152
TCAACGC235550.033.14244
CAACGCA241850.032.2790685
AACGCAG251600.031.101186
TATAACG3250.028.9409282
ACGCAGA279150.027.9475487
ATAACGC3200.027.9230353
CGCAGAG280500.027.7795318
GTACATG155700.026.3109261
GCAGAGT300100.025.6206769
TACATGG154850.025.5113752
CATGGGT76650.024.909954
GCGGTAT2101.4206307E-924.6365571
ACATGGG158800.024.609883
GAGTACT212850.023.69186812-13
Produced by FastQC (version 0.11.5)