##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431410_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3193036
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.624784061313434	34.0	31.0	34.0	31.0	34.0
2	32.73914136890408	34.0	31.0	34.0	31.0	34.0
3	32.81743206152389	34.0	31.0	34.0	31.0	34.0
4	36.29377276046997	37.0	37.0	37.0	35.0	37.0
5	36.1988615223881	37.0	37.0	37.0	35.0	37.0
6	36.25054744136928	37.0	37.0	37.0	35.0	37.0
7	36.20536630341781	37.0	37.0	37.0	35.0	37.0
8	36.22218415326354	37.0	37.0	37.0	35.0	37.0
9	38.00877033644469	39.0	39.0	39.0	35.0	39.0
10-11	37.989192730680145	39.0	38.5	39.0	35.0	39.0
12-13	37.97238944377702	39.0	38.0	39.0	35.0	39.0
14-15	39.42748907309532	41.0	39.0	41.0	36.0	41.0
16-17	39.46377507174989	41.0	39.0	41.0	36.0	41.0
18-19	39.53791798777088	41.0	39.5	41.0	36.5	41.0
20-21	39.53684815955724	41.0	40.0	41.0	36.0	41.0
22-23	39.4772032009661	41.0	39.0	41.0	36.0	41.0
24-25	39.42949781962997	41.0	39.0	41.0	36.0	41.0
26-27	39.310088736863605	41.0	39.0	41.0	36.0	41.0
28-29	39.22459768696626	41.0	39.0	41.0	35.0	41.0
30-31	39.04110038220678	41.0	39.0	41.0	35.0	41.0
32-33	38.81135305082687	41.0	38.0	41.0	35.0	41.0
34-35	38.67347737388492	41.0	38.0	41.0	35.0	41.0
36-37	38.528625421072604	41.0	38.0	41.0	35.0	41.0
38-39	38.3750324142916	40.5	38.0	41.0	35.0	41.0
40-41	38.227117389218286	40.0	38.0	41.0	34.0	41.0
42-43	38.042359528674275	40.0	38.0	41.0	34.0	41.0
44-45	37.786612333841525	40.0	38.0	41.0	33.0	41.0
46-47	37.3997443185733	40.0	38.0	41.0	33.0	41.0
48-49	37.04528902899936	40.0	37.0	41.0	31.5	41.0
50-51	36.86607620459024	40.0	37.0	41.0	31.0	41.0
52-53	36.79816200005261	40.0	37.0	41.0	31.0	41.0
54-55	36.65367960148273	40.0	36.5	41.0	31.0	41.0
56-57	36.502399597123244	40.0	36.0	41.0	31.0	41.0
58-59	36.32347286407043	40.0	35.5	41.0	30.5	41.0
60-61	36.16749043856693	39.5	35.0	41.0	30.0	41.0
62-63	36.006527799874476	39.0	35.0	41.0	30.0	41.0
64-65	35.77576168887542	39.0	35.0	41.0	30.0	41.0
66-67	35.512453664788	38.5	35.0	41.0	29.0	41.0
68-69	35.21887100552577	37.5	35.0	41.0	29.0	41.0
70-71	34.91787878370303	37.0	35.0	40.0	29.0	41.0
72-73	34.59967316372255	37.0	35.0	39.5	29.0	41.0
74-75	34.26352975663287	36.0	35.0	39.0	28.0	41.0
76-77	33.928056871266094	36.0	35.0	39.0	28.0	41.0
78-79	33.609695286868046	35.0	34.0	37.5	28.0	39.5
80-81	33.345268891424965	35.0	34.0	37.0	28.0	39.0
82-83	33.14480419262419	35.0	34.0	37.0	29.0	39.0
84-85	32.88506393288394	35.0	34.0	36.0	28.0	38.0
86-87	32.64539500963973	35.0	34.0	36.0	28.0	37.0
88-89	32.41092850190226	35.0	34.0	36.0	27.0	37.0
90-91	32.23213252215133	35.0	34.0	35.5	27.0	36.5
92-93	32.07392040052164	35.0	34.0	35.0	27.0	36.0
94-95	31.93761204070358	35.0	34.0	35.0	26.5	36.0
96-97	31.79833894763479	35.0	34.0	35.0	26.0	36.0
98-99	31.687777557158768	35.0	34.0	35.0	26.0	36.0
100	31.584529895685485	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.010587517649526035
1101	2	0.04740163018758636
1101	3	0.009297842335449502
1101	4	0.03607334388036065
1101	5	0.020353346891837987
1101	6	-0.013694534551490278
1101	7	0.03728612486991523
1101	8	0.04036057290355899
1101	9	0.050408472944390326
1101	10-11	0.03600613476480419
1101	12-13	0.0529492261579847
1101	14-15	0.07081429797111838
1101	16-17	0.03696357738265732
1101	18-19	0.006582457349921356
1101	20-21	0.027405279484398193
1101	22-23	0.04212041138652012
1101	24-25	0.05339826466490649
1101	26-27	0.08222282187953311
1101	28-29	0.05613625010913381
1101	30-31	-0.007246646715074689
1101	32-33	-0.056684513120941915
1101	34-35	-0.0493446347063653
1101	36-37	-0.1489950605331174
1101	38-39	-0.05615745996730226
1101	40-41	-0.12423224626243723
1101	42-43	-0.11873379137644235
1101	44-45	-0.16194509263749524
1101	46-47	-0.22847637350363215
1101	48-49	-0.2151991460286382
1101	50-51	-0.20688765747286908
1101	52-53	-0.10399338472010555
1101	54-55	-0.14848753685426175
1101	56-57	-0.1407649869214822
1101	58-59	-0.19543342606212377
1101	60-61	-0.15257286451389263
1101	62-63	-0.09310549714635385
1101	64-65	-0.08662211418339183
1101	66-67	-0.09246802831012246
1101	68-69	-0.14051063866309477
1101	70-71	-0.12881050097621483
1101	72-73	-0.22538051792014357
1101	74-75	-0.1568963059487487
1101	76-77	-0.1769744555243804
1101	78-79	-0.16926317511325806
1101	80-81	-0.1440793521925201
1101	82-83	-0.17426226068727857
1101	84-85	-0.09438706984528977
1101	86-87	-0.03818707195690507
1101	88-89	-0.03682102352326666
1101	90-91	-0.09408643403058647
1101	92-93	-0.06118761839508835
1101	94-95	-0.07403840843475606
1101	96-97	-0.04737856297788312
1101	98-99	-0.061079986090511795
1101	100	-0.030604013660749274
1102	1	0.0029655166349513706
1102	2	0.007475602486557875
1102	3	0.03354678199377048
1102	4	0.03902874921132593
1102	5	0.01080691302654202
1102	6	-0.015839136170541224
1102	7	-0.06757695408756348
1102	8	-0.022335738778664904
1102	9	0.012196611017643022
1102	10-11	-0.004298494245198015
1102	12-13	-0.008514655514233027
1102	14-15	-0.012145946799456908
1102	16-17	-0.01016552152623973
1102	18-19	-0.02763614632066691
1102	20-21	0.029237316528195834
1102	22-23	0.05232126207725685
1102	24-25	0.053127469892714885
1102	26-27	0.08617468223961566
1102	28-29	0.024931952537997404
1102	30-31	0.07849832132532697
1102	32-33	0.10318511953491338
1102	34-35	0.12315275090375621
1102	36-37	0.17098882659683312
1102	38-39	0.1770375162201887
1102	40-41	0.12019613386647165
1102	42-43	0.14821869355493789
1102	44-45	0.1840138474369013
1102	46-47	0.11767426137645742
1102	48-49	0.14675125971025693
1102	50-51	0.11714341442581144
1102	52-53	0.15436572447898556
1102	54-55	0.21128305232311106
1102	56-57	0.2228478197884698
1102	58-59	0.2555612042983597
1102	60-61	0.1949385315296226
1102	62-63	0.2855406731066239
1102	64-65	0.2738070525287952
1102	66-67	0.20129390612685683
1102	68-69	0.20539404948011253
1102	70-71	0.23985573133408877
1102	72-73	0.24816385558045795
1102	74-75	0.29419876254141286
1102	76-77	0.2728602201994619
1102	78-79	0.28295261182680775
1102	80-81	0.22190833629168338
1102	82-83	0.2152709053963875
1102	84-85	0.20008390525530473
1102	86-87	0.17599476405954562
1102	88-89	0.11467398443902965
1102	90-91	0.14932459454558966
1102	92-93	0.15958071353933434
1102	94-95	0.12913728205123576
1102	96-97	0.15445737908923718
1102	98-99	0.17412754149277987
1102	100	0.1585922744286279
1103	1	-0.04872407620840136
1103	2	-0.07037118506532636
1103	3	-0.05755567160660746
1103	4	-0.020518528959286186
1103	5	-0.004992765084821826
1103	6	-0.004443301834236024
1103	7	-0.04614779527097568
1103	8	-0.029966254668892134
1103	9	-0.03201007308945236
1103	10-11	-0.003295488891673415
1103	12-13	0.06222245655916936
1103	14-15	-0.03707861024938808
1103	16-17	-0.022624482107438837
1103	18-19	0.006563035344164803
1103	20-21	-0.024907788583163892
1103	22-23	-0.027284741074367957
1103	24-25	-0.029935760930285937
1103	26-27	-0.05313797703179546
1103	28-29	-0.06866192462143772
1103	30-31	-0.0753484828116271
1103	32-33	-0.026180849230328818
1103	34-35	-0.11163750435203212
1103	36-37	-0.0995441278289988
1103	38-39	-0.12112478853375563
1103	40-41	-0.1642141274106237
1103	42-43	-0.1382688021058769
1103	44-45	-0.1151724243606651
1103	46-47	-0.1295883146586192
1103	48-49	-0.13008653343379706
1103	50-51	-0.1999663693163427
1103	52-53	-0.14178280683213274
1103	54-55	-0.0919993640668082
1103	56-57	-0.11858197018231209
1103	58-59	-0.2084018496862825
1103	60-61	-0.21128946301480767
1103	62-63	-0.2235055630837124
1103	64-65	-0.2485444667215262
1103	66-67	-0.16350033645701956
1103	68-69	-0.13155512164629357
1103	70-71	-0.1557122993704141
1103	72-73	-0.1640235244225039
1103	74-75	-0.18869483983559832
1103	76-77	-0.1044436595790259
1103	78-79	-0.17241875215497515
1103	80-81	-0.13972559750577318
1103	82-83	-0.15406175040597958
1103	84-85	-0.17382330540484503
1103	86-87	-0.16936561922150162
1103	88-89	-0.13516222644293663
1103	90-91	-0.11689244002945287
1103	92-93	-0.164078861242416
1103	94-95	-0.1298981853587442
1103	96-97	-0.11081951216773689
1103	98-99	-0.13216257797272846
1103	100	-0.0611841357460392
1104	1	-0.06817703943910658
1104	2	-0.07229155588807146
1104	3	-0.04013516485739643
1104	4	-0.019143174652228367
1104	5	-0.019294533575056505
1104	6	-0.0373225451294914
1104	7	-0.002688253335236368
1104	8	-0.11502010663040352
1104	9	-0.08570167979443966
1104	10-11	-0.08691358231913426
1104	12-13	-0.018966609575208793
1104	14-15	-0.08331272419756885
1104	16-17	0.016081791774318788
1104	18-19	-0.027389292546153854
1104	20-21	0.047280618370038496
1104	22-23	-0.016336570974431197
1104	24-25	0.04479906948479595
1104	26-27	-0.030483476781299146
1104	28-29	-0.032642927006193645
1104	30-31	-0.03129852514882714
1104	32-33	0.0353501323982357
1104	34-35	0.017319599607638736
1104	36-37	-0.02085956602403627
1104	38-39	-0.012998691263739204
1104	40-41	-0.038377479151463945
1104	42-43	-0.04369930707662917
1104	44-45	0.021303028541098
1104	46-47	-0.002206805131450551
1104	48-49	-0.03769333061547542
1104	50-51	-0.13584559882687586
1104	52-53	-0.137880169497258
1104	54-55	-0.022951185989818157
1104	56-57	-0.06753257950119007
1104	58-59	-0.025086184156215552
1104	60-61	0.04407703336150348
1104	62-63	-0.08633587963304734
1104	64-65	-0.05127888223887567
1104	66-67	-0.11172505554798562
1104	68-69	-0.10923868620525212
1104	70-71	-0.12076164078807494
1104	72-73	-0.15319751865144582
1104	74-75	-0.10803933307396107
1104	76-77	-0.11436631369510764
1104	78-79	-0.08271388770518229
1104	80-81	-0.06425902400278005
1104	82-83	-0.00524636701087644
1104	84-85	-0.02361575485387135
1104	86-87	-0.04321998966957352
1104	88-89	0.011756107177767205
1104	90-91	-0.03577295287557547
1104	92-93	0.011866968843406767
1104	94-95	0.028230680155861165
1104	96-97	0.04029107366739382
1104	98-99	0.050477003134240306
1104	100	0.11908842674680287
1105	1	-0.11736066880751395
1105	2	-0.10864405334283589
1105	3	-0.10680220282102937
1105	4	-0.009390168817667188
1105	5	-0.10181312998342662
1105	6	0.009858753979173684
1105	7	-0.027848820706694255
1105	8	-0.06289023586402465
1105	9	-0.05255451475813544
1105	10-11	-0.07107238305965069
1105	12-13	-0.11015216820262452
1105	14-15	-0.05233029471242645
1105	16-17	-0.0865320645827623
1105	18-19	-0.14069616725793566
1105	20-21	-0.060717675149120964
1105	22-23	-0.1254591145665529
1105	24-25	-0.12443036307691102
1105	26-27	-0.038181019760770596
1105	28-29	-0.11081378450167989
1105	30-31	-0.1163899867296152
1105	32-33	-0.1090377511422389
1105	34-35	-0.13019085425834476
1105	36-37	-0.07436364397857886
1105	38-39	-0.058184789014354976
1105	40-41	-0.0966875507300955
1105	42-43	-0.16754080485887357
1105	44-45	-0.14881963312345903
1105	46-47	-0.08049974506823077
1105	48-49	-0.1479791104616197
1105	50-51	-0.07408859189624195
1105	52-53	-0.036280288015241524
1105	54-55	-0.07737823393391352
1105	56-57	-0.00461077686929201
1105	58-59	-0.014906001166224314
1105	60-61	-0.07835917967681638
1105	62-63	-0.09500094823758332
1105	64-65	-0.12008525904143141
1105	66-67	-0.10312381289130457
1105	68-69	-0.07873779175087492
1105	70-71	-0.03692887591039096
1105	72-73	0.09198701965191702
1105	74-75	-0.007531287440080803
1105	76-77	-0.03947062957674774
1105	78-79	0.019388915512635663
1105	80-81	0.07575538974531071
1105	82-83	-0.002867653020189209
1105	84-85	-0.031197787348297368
1105	86-87	-0.035346674708016224
1105	88-89	0.024214976756731232
1105	90-91	-0.0010784820071663148
1105	92-93	0.009756591167558781
1105	94-95	-0.04297368094973919
1105	96-97	-0.017145707455270554
1105	98-99	0.009518344080646557
1105	100	-0.013191685622512495
1106	1	-0.11226984299925391
1106	2	-0.1363976511969156
1106	3	-0.1754076781350946
1106	4	-0.02848514661329915
1106	5	-0.05604228394442856
1106	6	-0.12600553346396737
1106	7	-0.0414076707467359
1106	8	-0.04434691890671871
1106	9	-0.04457742442855306
1106	10-11	-0.10527392361526466
1106	12-13	-0.12166538802286198
1106	14-15	-0.17541433818944796
1106	16-17	-0.17921438202674977
1106	18-19	-0.15746786260651646
1106	20-21	-0.13553542927617457
1106	22-23	-0.11859614663298146
1106	24-25	-0.08457826786748512
1106	26-27	-0.14254325246466237
1106	28-29	-0.1600877072275182
1106	30-31	-0.21680314677719537
1106	32-33	-0.12435230365845484
1106	34-35	-0.06605540195978676
1106	36-37	-0.055402265442424437
1106	38-39	-0.0374795931309464
1106	40-41	-0.1295065124556416
1106	42-43	-0.10723553055096602
1106	44-45	-0.11434538524824944
1106	46-47	-0.09500528466609381
1106	48-49	-0.006342293975819757
1106	50-51	-0.06583155039111688
1106	52-53	-6.317707320846466E-5
1106	54-55	0.036154980663802405
1106	56-57	0.034204579411309055
1106	58-59	0.10402553441846152
1106	60-61	0.05808201365953636
1106	62-63	0.014870041724250882
1106	64-65	-0.019825087496514016
1106	66-67	0.013350580728541672
1106	68-69	-0.026845453668407515
1106	70-71	-0.07216689513654018
1106	72-73	0.00693447783925194
1106	74-75	0.019604023451471164
1106	76-77	-0.029755447582459738
1106	78-79	0.008373960203314823
1106	80-81	-0.033566845595856876
1106	82-83	-0.05562825616011935
1106	84-85	-0.032884955139429906
1106	86-87	-0.022943154627789397
1106	88-89	-0.09041908706380042
1106	90-91	-0.1187441646082874
1106	92-93	-0.05332672358621693
1106	94-95	-0.004216796033357895
1106	96-97	-0.06742058359401426
1106	98-99	-0.08013105329126802
1106	100	-0.061196675641603804
1107	1	-0.1546140217306089
1107	2	-0.10824320169794532
1107	3	-0.12118533838827972
1107	4	-0.17439970651939518
1107	5	-0.21402622408948702
1107	6	-0.161155952853548
1107	7	-0.1269884272495716
1107	8	-0.10705940070953801
1107	9	-0.13494698096292979
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2216	26-27	-0.14220236810646725
2216	28-29	-0.13497910438508143
2216	30-31	-0.14437065001426674
2216	32-33	-0.10142732712115787
2216	34-35	-0.09525769702403863
2216	36-37	-0.06113408212663529
2216	38-39	-0.030308938831986154
2216	40-41	-0.003346763297251698
2216	42-43	0.0018408303882750943
2216	44-45	-0.01639564296677065
2216	46-47	0.022887475870959406
2216	48-49	0.08683751543534868
2216	50-51	0.1782370301874252
2216	52-53	0.050214324037931135
2216	54-55	0.09273282075606915
2216	56-57	0.0606406687865757
2216	58-59	0.07530935395325855
2216	60-61	0.028104525531453817
2216	62-63	0.03532930254891653
2216	64-65	0.07226265210179861
2216	66-67	0.07526584934684166
2216	68-69	0.10560705312084906
2216	70-71	0.07599780211645424
2216	72-73	0.127094851827259
2216	74-75	0.1362411623383295
2216	76-77	0.14400910031793757
2216	78-79	0.09821679091524516
2216	80-81	0.054525324985789325
2216	82-83	0.0669906865384533
2216	84-85	0.15566598979690838
2216	86-87	0.10625920361438546
2216	88-89	0.11658502234392643
2216	90-91	0.1561402355947621
2216	92-93	0.11971814884650911
2216	94-95	0.09384883824293766
2216	96-97	0.10918207436420957
2216	98-99	0.102115959419816
2216	100	0.08480441480927325
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41.0
3	299.0
4	72.0
5	105.0
6	267.0
7	576.0
8	1135.0
9	1933.0
10	2934.0
11	4106.0
12	6514.0
13	9452.0
14	11061.0
15	14690.0
16	18872.0
17	29019.0
18	34094.0
19	17232.0
20	10285.0
21	8888.0
22	8512.0
23	8563.0
24	9247.0
25	10872.0
26	13518.0
27	17159.0
28	21424.0
29	27042.0
30	34332.0
31	43262.0
32	54941.0
33	71278.0
34	99437.0
35	163170.0
36	263572.0
37	546591.0
38	994231.0
39	628030.0
40	6280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66673191644229	20.788766431589995	14.849919774095882	22.69458187787184
2	14.53320521581934	21.932618038386394	40.22093380880465	23.31324293698961
3	16.547839134507548	27.919767065674538	32.149327764490046	23.38306603532787
4	11.238833511429249	18.565371640031618	39.50371997058599	30.692074877953146
5	11.391451249765801	38.87188655138079	35.52864006155932	14.208022137294085
6	27.63194652362203	39.91242190817767	18.292433909295102	14.16319765890519
7	24.12387458205921	35.20536567705469	23.372771243418487	17.297988497467614
8	24.941497684335534	34.8598950967042	21.182692584737538	19.015914634222728
9	25.685476571812266	15.714865547939672	22.240508557065162	36.359149323182905
10-11	22.682393809527984	27.447654833832125	29.541759003030343	20.32819235360954
12-13	24.512125763693238	25.838293085326942	29.628995726950773	20.020585424029044
14-15	21.795369673251415	26.85528756957328	28.59197328185464	22.757369475320665
16-17	19.303775466358665	30.0820285145548	29.158581362690555	21.455614656395984
18-19	19.158875358221152	27.62400468895654	33.40516677787227	19.811953174950037
20-21	20.90036595276907	26.629100526614963	33.227055137197056	19.24347838341891
22-23	20.306952524315037	26.686761449022868	33.42716779289419	19.579118233767907
24-25	20.00708417135552	26.82871525953059	33.310324913546175	19.853875655567712
26-27	19.814311200597675	26.760001196355628	33.41828397818311	20.00740362486359
28-29	19.749304266725627	26.867393206586588	33.463126293049186	19.9201762336386
30-31	20.01756327696674	27.218992266270302	33.086686422221426	19.67675803454153
32-33	19.733855308487318	26.968493579705605	33.46165048543689	19.836000626370183
34-35	19.967757695716582	26.892869110964263	33.127205990585686	20.012167202733472
36-37	20.235054733775527	27.067498569527537	32.69104832726328	20.006398369433644
38-39	20.159952596430607	27.19310017496039	32.716683967487086	19.930263261121915
40-41	20.051098807574437	27.049045428487272	32.731250995332175	20.168604768606116
42-43	20.04477898012412	27.104843821640728	32.520068800156345	20.330308398078806
44-45	20.405336314829732	27.266943363312716	32.06452183703398	20.263198484823572
46-47	20.556208714737235	27.59769096919914	31.425530875394763	20.420569440668856
48-49	20.668052080320585	27.656869440135146	30.88017194955449	20.794906529989778
50-51	20.690333375352036	27.755676635538894	30.62461977640308	20.929370212705987
52-53	20.69703064438136	27.939885049119706	30.500363919253772	20.86272038724516
54-55	20.851336233841238	28.195611405435134	30.30495733798016	20.648095022743473
56-57	20.724867783694076	27.818752361634637	30.37311517479737	21.083264679873917
58-59	20.558315223501786	27.49612670399187	30.766685551627194	21.178872520879153
60-61	20.723601741873118	27.807317226325907	30.480101860335402	20.988979171465576
62-63	20.59480066175958	27.693417089400963	30.704626758043567	21.00715549079589
64-65	20.541653793375815	27.501671244155734	30.83235240553458	21.124322556933876
66-67	20.515245674119537	27.604614502161823	30.6159951055748	21.26414471814384
68-69	20.97489058181505	27.70075398720649	30.347601412453706	20.976754018524755
70-71	20.95636657743564	27.57340419618089	30.26445945808399	21.205769768299472
72-73	20.755031935961775	27.67419540777954	30.412691015635463	21.158081640623227
74-75	20.840711429027785	27.798832449632165	30.17713693348909	21.183319187850962
76-77	20.95014779948127	27.764285625923012	30.028560838469424	21.25700573612629
78-79	21.03387761103674	27.800380111734746	30.106340149110157	21.059402128118357
80-81	20.912634160243783	27.713568075264405	30.155652503437196	21.218145261054616
82-83	20.735921964003623	27.704410414320378	30.439403467848926	21.120264153827073
84-85	20.727799562448354	27.6987636527404	30.169077961142744	21.404358823668503
86-87	20.841345073498175	27.80248048016729	30.034445980631514	21.321728465703018
88-89	20.643857627423337	27.67242976290081	30.114248767259888	21.569463842415967
90-91	20.54251615060167	27.62214443034238	30.052419477966204	21.782919941089744
92-93	20.54602576668114	27.713753165323073	30.03447072259172	21.705750345404063
94-95	20.379503741295053	27.550021734963725	30.249510493540267	21.82096403020095
96-97	20.325311592652877	27.612228005302796	30.137524502580774	21.924935899463552
98-99	20.32781437924176	27.541769540591034	30.088162437737363	22.042253642429838
100	20.48370341534423	27.592873354502352	29.850853311477405	22.072569918676017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3803.0
1	2133.0
2	1936.5
3	3343.5
4	2005.5
5	1138.5
6	2926.0
7	7063.5
8	12411.5
9	13775.5
10	8739.5
11	4084.5
12	3331.0
13	3578.5
14	3932.0
15	4343.5
16	4810.5
17	5447.0
18	6159.0
19	6935.0
20	8001.5
21	9566.5
22	12019.5
23	16085.5
24	20566.5
25	25493.5
26	32597.0
27	41415.0
28	51656.0
29	61142.0
30	68497.5
31	76053.5
32	83655.0
33	90689.5
34	97073.5
35	102504.0
36	105834.5
37	107391.5
38	108816.5
39	109282.0
40	109270.0
41	107911.5
42	104462.5
43	100808.0
44	98082.5
45	95590.5
46	93069.0
47	90731.0
48	88352.5
49	86907.5
50	86029.5
51	83032.0
52	78581.0
53	74460.5
54	71085.0
55	68120.5
56	62793.5
57	56237.0
58	51253.0
59	47160.5
60	41438.0
61	34860.5
62	29660.5
63	24451.0
64	19232.5
65	15158.0
66	11778.5
67	8818.5
68	6681.5
69	5127.5
70	3788.5
71	2762.5
72	2046.0
73	1530.5
74	1146.5
75	810.5
76	526.0
77	381.5
78	268.5
79	131.5
80	63.5
81	38.5
82	22.5
83	9.5
84	6.5
85	6.0
86	6.5
87	5.0
88	1.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005543313636301
2	0.004196632922397367
3	0.001033499152530695
4	0.0
5	0.04127732978895321
6	0.0
7	0.0
8	0.0
9	6.263631227458758E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.697723420594068E-5
20-21	3.131815613729379E-5
22-23	4.697723420594068E-5
24-25	6.263631227458758E-5
26-27	1.7224985875511582E-4
28-29	6.263631227458758E-5
30-31	3.131815613729378E-4
32-33	0.0011274536209425763
34-35	8.142720595696385E-4
36-37	0.0010961354648052824
38-39	8.612492937755791E-4
40-41	2.0356801489240963E-4
42-43	6.263631227458756E-4
44-45	2.662043271669972E-4
46-47	2.505452490983503E-4
48-49	2.0356801489240963E-4
50-51	4.2279510785346613E-4
52-53	6.263631227458756E-4
54-55	0.0010021809963934012
56-57	0.0021139755392673306
58-59	5.950449666085819E-4
60-61	0.0011431126990112232
62-63	0.0010491582305993418
64-65	0.001738157665619805
66-67	4.854314201280537E-4
68-69	3.4449971751023163E-4
70-71	4.2279510785346613E-4
72-73	1.7224985875511582E-4
74-75	8.142720595696385E-4
76-77	8.612492937755791E-4
78-79	8.612492937755791E-4
80-81	8.142720595696385E-4
82-83	0.0015032714945901018
84-85	0.001299703479697692
86-87	0.002051339226992743
88-89	0.0014876124165214548
90-91	0.0013623397919722796
92-93	0.0011431126990112232
94-95	7.516357472950509E-4
96-97	1.565907806864689E-4
98-99	2.9752248330429094E-4
100	1.565907806864689E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3193036.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.23235251563281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59454360031621	49.19847725309519
2	15.372023702184245	19.74762490634723
3	4.2811706985274425	8.24968996462238
4	1.5790134462808747	4.056949932337496
5	0.7263760798078863	2.3328422208571795
6	0.3960890382641799	1.526503844001767
7	0.2408116842622527	1.0827530695391399
8	0.15850042624981628	0.814468420220501
9	0.10387112968409075	0.6004698316258976
>10	0.4970583404631013	5.581001734515826
>50	0.029592964898767428	1.3440497193551193
>100	0.01899676226069128	2.232842158533459
>500	0.0011224729123630703	0.45945919701019616
>1k	6.832443783994697E-4	0.9891993155234882
>5k	0.0	0.0
>10k+	1.464095096570292E-4	1.78366843241496
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24206	0.7580872874593334	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19978	0.6256741233108553	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12716	0.39824167344182776	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4667	0.1461618346927501	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4374	0.13698561494452302	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4208	0.13178680102573226	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4053	0.1269324868244517	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3248	0.10172137113393022	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	9.395446841188136E-5	0.0
9	0.0	0.0	0.0	1.2527262454917513E-4	0.0
10-11	0.0	0.0	0.0	1.2527262454917513E-4	0.0
12-13	0.0	0.0	0.0	1.2527262454917513E-4	0.0
14-15	0.0	0.0	0.0	1.2527262454917513E-4	0.0
16-17	0.0	0.0	0.0	1.2527262454917513E-4	0.0
18-19	0.0	0.0	0.0	1.2527262454917513E-4	0.0
20-21	0.0	0.0	0.0	1.5659078068646894E-4	0.0
22-23	0.0	0.0	0.0	3.7581787364752544E-4	0.0
24-25	0.0	0.0	0.0	5.637268104712881E-4	0.0
26-27	0.0	0.0	0.0	0.0010491582305993418	0.0
28-29	0.0	0.0	0.0	0.0024897934129148556	0.0
30-31	0.0	0.0	0.0	0.006028745056429054	0.0
32-33	0.0	0.0	0.0	0.012918739406633687	0.0
34-35	0.0	0.0	0.0	0.024021025757304332	0.0
36-37	0.0	0.0	0.0	0.041715783974875326	0.0
38-39	0.0	0.0	0.0	0.06623790023037636	0.0
40-41	0.0	0.0	0.0	0.0932341508207236	0.0
42-43	0.0	0.0	0.0	0.1179441760130484	0.0
44-45	0.0	0.0	0.0	0.15034280853707882	0.0
46-47	0.0	0.0	0.0	0.19046136654895215	0.0
48-49	0.0	0.0	0.0	0.23440073960957533	0.0
50-51	0.0	0.0	0.0	0.2802505201945734	0.0
52-53	0.0	0.0	0.0	0.3250511425489722	0.0
54-55	0.0	0.0	0.0	0.3749409652756812	0.0
56-57	0.0	0.0	0.0	0.43638718761705164	0.0
58-59	0.0	0.0	0.0	0.5099691954616233	0.0
60-61	0.0	0.0	0.0	0.5877165180724552	0.0
62-63	0.0	0.0	0.0	0.6669357940217397	0.0
64-65	0.0	0.0	0.0	0.7525283147449637	0.0
66-67	0.0	0.0	0.0	0.8554084576559737	0.0
68-69	0.0	0.0	0.0	0.9773926758107331	0.0
70-71	0.0	0.0	0.0	1.1128593601825973	0.0
72-73	0.0	0.0	0.0	1.254292153298616	0.0
74-75	0.0	0.0	0.0	1.3983243533740302	0.0
76-77	0.0	0.0	0.0	1.559973016276672	0.0
78-79	0.0	0.0	0.0	1.7476157487732678	0.0
80-81	0.0	0.0	0.0	1.9633978445592222	0.0
82-83	0.0	0.0	0.0	2.1851930263235366	0.0
84-85	0.0	0.0	0.0	2.4101200237015803	0.0
86-87	0.0	0.0	0.0	2.6544329597286094	0.0
88	0.0	0.0	0.0	2.8554015676616236	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9415	0.0	64.99255	1
GTATCAA	17995	0.0	43.648697	1
GTGGTAT	3135	0.0	36.156475	1
TGGTATC	3160	0.0	33.783493	2
ATCAACG	23225	0.0	33.613316	3
TATCAAC	23355	0.0	33.48715	2
TCAACGC	23555	0.0	33.1424	4
CAACGCA	24185	0.0	32.279068	5
AACGCAG	25160	0.0	31.10118	6
TATAACG	325	0.0	28.940928	2
ACGCAGA	27915	0.0	27.947548	7
ATAACGC	320	0.0	27.923035	3
CGCAGAG	28050	0.0	27.779531	8
GTACATG	15570	0.0	26.310926	1
GCAGAGT	30010	0.0	25.620676	9
TACATGG	15485	0.0	25.511375	2
CATGGGT	7665	0.0	24.90995	4
GCGGTAT	210	1.4206307E-9	24.636557	1
ACATGGG	15880	0.0	24.60988	3
GAGTACT	21285	0.0	23.691868	12-13
>>END_MODULE