FastQCFastQC Report
Wed 25 May 2016
SRR2431409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4649506
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA187580.40344070961517203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54000.047.5851171
GTATCAA95300.036.1485141
TCAACGC104750.031.3557724
ATCAACG104850.031.3272153
CAACGCA113300.029.0295285
TATCAAC116300.029.011772
AACGCAG118600.027.775986
GTACATG203300.026.8993021
TACATGG208000.025.2937552
CGCAGAG134550.024.0993968
ACGCAGA135750.024.0597137
ACATGGG212850.023.9878253
CATGGGT94000.022.7771634
GTGGTAT31950.020.3273371
GCAGAGT154400.020.1186879
GTACCTG36600.019.6735441
TACCTGG33700.019.409742
CATGGGG101250.018.2182714
ATGGGTG52100.017.701545
TGGTATC31650.017.6932682
Produced by FastQC (version 0.11.5)