##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431409_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4649506
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.920071508672105	33.0	31.0	34.0	27.0	34.0
2	30.90256169150013	33.0	31.0	34.0	27.0	34.0
3	31.057146286078563	34.0	31.0	34.0	27.0	34.0
4	34.30727436420127	37.0	35.0	37.0	30.0	37.0
5	34.347859536045334	37.0	35.0	37.0	31.0	37.0
6	34.40019541861006	37.0	35.0	37.0	31.0	37.0
7	34.25377534731647	37.0	35.0	37.0	30.0	37.0
8	34.25129400843875	37.0	35.0	37.0	30.0	37.0
9	35.72714692700687	39.0	37.0	39.0	30.0	39.0
10-11	35.74995171530051	39.0	37.0	39.0	30.0	39.0
12-13	35.694247410370046	39.0	37.0	39.0	30.0	39.0
14-15	37.05415639855073	41.0	38.0	41.0	30.0	41.0
16-17	37.07334241530175	40.5	38.0	41.0	30.0	41.0
18-19	37.09860638958203	40.5	38.0	41.0	30.0	41.0
20-21	37.184320334246266	41.0	38.0	41.0	30.0	41.0
22-23	37.15552609244939	40.0	38.0	41.0	30.0	41.0
24-25	37.199027057928305	40.5	38.0	41.0	30.0	41.0
26-27	37.23629370518073	40.0	38.0	41.0	30.0	41.0
28-29	37.19920686197631	40.0	38.0	41.0	30.0	41.0
30-31	37.13465075644595	40.0	38.0	41.0	30.0	41.0
32-33	37.083782879299434	40.0	38.0	41.0	30.0	41.0
34-35	37.012880078012586	40.0	38.0	41.0	30.0	41.0
36-37	36.877933161071304	40.0	38.0	41.0	30.0	41.0
38-39	36.75456984032282	40.0	37.0	41.0	30.0	41.0
40-41	36.69150238756548	40.0	37.0	41.0	30.0	41.0
42-43	36.52264219037464	40.0	37.0	41.0	29.0	41.0
44-45	36.368057380719584	40.0	36.5	41.0	29.0	41.0
46-47	36.235800857123316	40.0	36.0	41.0	28.5	41.0
48-49	36.09749627164692	40.0	36.0	41.0	28.0	41.0
50-51	35.96283594429171	40.0	35.5	41.0	28.0	41.0
52-53	35.80469097147095	40.0	35.0	41.0	28.0	41.0
54-55	35.63832426498644	39.5	35.0	41.0	28.0	41.0
56-57	35.449971674410136	39.0	35.0	41.0	27.5	41.0
58-59	35.24121691637778	39.0	35.0	41.0	27.0	41.0
60-61	35.00855327426183	39.0	34.0	41.0	26.5	41.0
62-63	34.91259469285554	38.5	34.0	41.0	26.5	41.0
64-65	34.66707075977534	38.0	34.0	40.0	26.0	41.0
66-67	34.405918714805395	37.5	34.0	40.0	26.0	41.0
68-69	34.10987091962028	37.0	34.0	40.0	26.0	41.0
70-71	33.77356540673354	36.5	33.5	39.5	25.0	41.0
72-73	33.425358091805876	36.0	33.0	39.0	25.0	41.0
74-75	33.1327894834419	36.0	33.0	39.0	24.0	41.0
76-77	32.858355382270716	35.0	33.0	38.5	24.0	40.0
78-79	32.50051285018236	35.0	33.0	37.0	23.0	39.5
80-81	32.16206721746353	35.0	33.0	37.0	22.5	39.0
82-83	31.824173471332223	35.0	32.5	36.5	21.5	39.0
84-85	31.512397123479353	35.0	32.0	36.0	20.0	37.5
86-87	31.219447937049658	35.0	32.0	36.0	19.5	37.0
88-89	30.94974777965659	35.0	32.0	35.5	18.0	37.0
90-91	30.716004990637714	35.0	32.0	35.0	16.0	36.0
92-93	30.479271991476082	35.0	32.0	35.0	8.5	36.0
94-95	30.263712962194262	35.0	31.0	35.0	2.0	36.0
96-97	30.056575042595924	35.0	31.0	35.0	2.0	36.0
98-99	29.83688890819799	35.0	31.0	35.0	2.0	35.0
100	29.66820389090798	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20364490924515977
1101	2	0.23334095252843667
1101	3	0.26523622947220815
1101	4	0.3411322074264831
1101	5	0.22498328151000635
1101	6	0.3167883368286297
1101	7	0.29271235600181456
1101	8	0.2856567494084743
1101	9	0.2680735537296641
1101	10-11	0.2420062310786193
1101	12-13	0.26021528720220743
1101	14-15	0.2669139630800714
1101	16-17	0.23573935588259332
1101	18-19	0.22573190036157342
1101	20-21	0.21813446239802659
1101	22-23	0.2828665611115184
1101	24-25	0.2865622016741014
1101	26-27	0.2707504684212907
1101	28-29	0.26343238172326977
1101	30-31	0.31627024956356564
1101	32-33	0.28858304716759164
1101	34-35	0.2774324665153216
1101	36-37	0.2722579519955417
1101	38-39	0.33111794896783664
1101	40-41	0.24511166016688435
1101	42-43	0.22771106917068096
1101	44-45	0.2319886624636851
1101	46-47	0.2181404864773384
1101	48-49	0.20785884969817658
1101	50-51	0.21461100362616747
1101	52-53	0.16463978333599272
1101	54-55	0.2169300756646777
1101	56-57	0.1573390554772942
1101	58-59	0.14542284175772124
1101	60-61	0.12752851245287644
1101	62-63	0.1469645957240715
1101	64-65	0.15663883040150495
1101	66-67	0.14663811792563308
1101	68-69	0.15184779991776765
1101	70-71	0.17070751696801523
1101	72-73	0.16982589340772591
1101	74-75	0.18340350422733565
1101	76-77	0.22755747668268356
1101	78-79	0.18959568947016692
1101	80-81	0.24325171701762827
1101	82-83	0.17154193911580506
1101	84-85	0.16350263535371923
1101	86-87	0.10124296458331727
1101	88-89	0.12162110085863631
1101	90-91	0.11628375376446698
1101	92-93	0.06327935986048772
1101	94-95	0.05489324880815616
1101	96-97	0.03325716744555862
1101	98-99	0.02218742034924759
1101	100	0.09076150730502164
1102	1	0.15314449765863003
1102	2	0.14163916875845572
1102	3	0.14312918043917833
1102	4	0.22771480997013782
1102	5	0.18007487140617684
1102	6	0.27518222837453266
1102	7	0.1206458757383686
1102	8	0.15542871562755778
1102	9	0.17928303586133154
1102	10-11	0.1559109580253022
1102	12-13	0.11124798311872297
1102	14-15	0.1810936043318847
1102	16-17	0.1544352697397997
1102	18-19	0.15869177076985608
1102	20-21	0.19497510857556222
1102	22-23	0.16066346570679002
1102	24-25	0.17805106600353326
1102	26-27	0.1593029967956383
1102	28-29	0.15928158007144333
1102	30-31	0.1410594042298996
1102	32-33	0.15495247909444743
1102	34-35	0.19733516806808638
1102	36-37	0.13561878722507004
1102	38-39	0.16898287285192026
1102	40-41	0.09210041850755601
1102	42-43	0.11422193425592297
1102	44-45	0.05752140347775736
1102	46-47	0.005933806102540018
1102	48-49	-0.04888613638627959
1102	50-51	-0.11153421734613289
1102	52-53	-0.0971073206209141
1102	54-55	-0.10362490396150292
1102	56-57	-0.05890713834627803
1102	58-59	-0.03199844969159216
1102	60-61	-0.003244021957314658
1102	62-63	0.062263731411384526
1102	64-65	0.12446481816161281
1102	66-67	0.11681550544148678
1102	68-69	0.024096088018445982
1102	70-71	-0.004239593306351708
1102	72-73	-0.07353254042465096
1102	74-75	-0.02414143063239038
1102	76-77	0.0011414515620131738
1102	78-79	-0.07879326608190951
1102	80-81	-0.07154205015797288
1102	82-83	-0.03964766774389261
1102	84-85	-0.06422369252185689
1102	86-87	-0.07432257205664072
1102	88-89	-0.04967591429223717
1102	90-91	-0.005578541141566973
1102	92-93	-0.04316780160054634
1102	94-95	-0.034797031850850146
1102	96-97	0.007713589698706613
1102	98-99	-0.07001757219728688
1102	100	-0.11241381714047805
1103	1	-0.28021485328796913
1103	2	-0.29141922698994094
1103	3	-0.17180045621650208
1103	4	-0.131595041201777
1103	5	-0.17347602029816755
1103	6	-0.17438542494937082
1103	7	-0.2925208145289062
1103	8	-0.28128290228708863
1103	9	-0.1842916285513212
1103	10-11	-0.1853058481633525
1103	12-13	-0.19788055464712073
1103	14-15	-0.1952445121422386
1103	16-17	-0.1410238183858823
1103	18-19	-0.18911876355422663
1103	20-21	-0.24791040924390728
1103	22-23	-0.24803582889447284
1103	24-25	-0.17698756559655493
1103	26-27	-0.21523081211839923
1103	28-29	-0.20153679598151797
1103	30-31	-0.22649781522119383
1103	32-33	-0.18293023512472217
1103	34-35	-0.20425006195051765
1103	36-37	-0.22699293444367186
1103	38-39	-0.19830940921799112
1103	40-41	-0.15195336733989961
1103	42-43	-0.2298864050598155
1103	44-45	-0.3396385702970903
1103	46-47	-0.4231855027501865
1103	48-49	-0.3612590774863733
1103	50-51	-0.47221806044625936
1103	52-53	-0.46841580283105344
1103	54-55	-0.43597188376077156
1103	56-57	-0.45714921262327834
1103	58-59	-0.4872630397072868
1103	60-61	-0.4436326840989935
1103	62-63	-0.329522374575717
1103	64-65	-0.3822580805902689
1103	66-67	-0.320228624783752
1103	68-69	-0.4051866060041718
1103	70-71	-0.33191118522363183
1103	72-73	-0.34077809898649036
1103	74-75	-0.3536184690130568
1103	76-77	-0.3476161283140229
1103	78-79	-0.32575666514641455
1103	80-81	-0.3678262737277258
1103	82-83	-0.3821360120176571
1103	84-85	-0.3453701828204885
1103	86-87	-0.3903116735112846
1103	88-89	-0.45016447533726733
1103	90-91	-0.41011756433457336
1103	92-93	-0.38119639035129893
1103	94-95	-0.4392932512613328
1103	96-97	-0.3598866233521356
1103	98-99	-0.42186305111182065
1103	100	-0.44553790862781995
1104	1	-0.10792914736454406
1104	2	-0.0761746138131123
1104	3	-0.047574374333908764
1104	4	0.013273185403214427
1104	5	0.0011709634014138715
1104	6	-0.0210587675090963
1104	7	0.015333394558950886
1104	8	-0.03187084080197877
1104	9	-0.06736758925042352
1104	10-11	-0.06329878063648664
1104	12-13	0.053257692087761654
1104	14-15	-0.07786083737204308
1104	16-17	-0.06482652460802996
1104	18-19	-0.030823455401595368
1104	20-21	-0.09019852164792042
1104	22-23	-0.014061526204741881
1104	24-25	-0.04508152379719377
1104	26-27	-0.02441902677461627
1104	28-29	-0.0059259633810455625
1104	30-31	-0.011603442529825259
1104	32-33	-0.05457018660487023
1104	34-35	-0.07227612302030195
1104	36-37	-0.13396697790247458
1104	38-39	-0.10510776333392613
1104	40-41	-0.0471899411061969
1104	42-43	-0.13231780991021935
1104	44-45	-0.1620679681984356
1104	46-47	-0.2549102803481844
1104	48-49	-0.24483195552101478
1104	50-51	-0.2641746778265244
1104	52-53	-0.3141335333433162
1104	54-55	-0.2827588140624968
1104	56-57	-0.31450076691704965
1104	58-59	-0.370567569605015
1104	60-61	-0.373042153274902
1104	62-63	-0.35734564552173254
1104	64-65	-0.31207200575057215
1104	66-67	-0.19239114956283743
1104	68-69	-0.23139227368406523
1104	70-71	-0.2816189116523091
1104	72-73	-0.2358602956294007
1104	74-75	-0.20994019595556068
1104	76-77	-0.2869480790824426
1104	78-79	-0.2845380428890678
1104	80-81	-0.29630472991932777
1104	82-83	-0.2976553495758054
1104	84-85	-0.29924820538101216
1104	86-87	-0.2699174938493911
1104	88-89	-0.3360622435820346
1104	90-91	-0.3332847715302272
1104	92-93	-0.36740588610069835
1104	94-95	-0.3130147852208154
1104	96-97	-0.3371350683150425
1104	98-99	-0.3925073793204632
1104	100	-0.3877069513456384
1105	1	-0.2071368543994545
1105	2	-0.1803021863324723
1105	3	-0.12495962650530146
1105	4	-0.03828096253673152
1105	5	-0.057319764892568514
1105	6	-0.06926202086632571
1105	7	-0.08965221245830435
1105	8	-0.08298969153588587
1105	9	-0.1169433809776308
1105	10-11	-0.18447241975280804
1105	12-13	-0.11001389399178407
1105	14-15	-0.16024493538916573
1105	16-17	-0.14411890356893053
1105	18-19	-0.13277714439549726
1105	20-21	-0.14161258694137047
1105	22-23	-0.21804284927289075
1105	24-25	-0.22480563566416834
1105	26-27	-0.19813090008015877
1105	28-29	-0.23968933233475553
1105	30-31	-0.20231974235647243
1105	32-33	-0.20606877672621238
1105	34-35	-0.23862760373235403
1105	36-37	-0.3105026820044827
1105	38-39	-0.24332526257580867
1105	40-41	-0.28653738001523266
1105	42-43	-0.37850468785501334
1105	44-45	-0.39546461028840696
1105	46-47	-0.37245563182210617
1105	48-49	-0.4557831823191165
1105	50-51	-0.3439042210077403
1105	52-53	-0.3157124050783082
1105	54-55	-0.41070988471031455
1105	56-57	-0.4691098028135201
1105	58-59	-0.42661782736613674
1105	60-61	-0.5041145323263194
1105	62-63	-0.41261356772720603
1105	64-65	-0.4277785178963498
1105	66-67	-0.43550379049412413
1105	68-69	-0.39397764715481287
1105	70-71	-0.3810513958901893
1105	72-73	-0.370630947070282
1105	74-75	-0.41435596758689996
1105	76-77	-0.4573153377623598
1105	78-79	-0.4349146705860534
1105	80-81	-0.3838662282378813
1105	82-83	-0.341343426935822
1105	84-85	-0.44154909113784413
1105	86-87	-0.49788751752086924
1105	88-89	-0.4563536335911351
1105	90-91	-0.4947951973905269
1105	92-93	-0.5567230567480692
1105	94-95	-0.5514985885891335
1105	96-97	-0.5326953083791324
1105	98-99	-0.5622580141053177
1105	100	-0.5545648455345855
1106	1	-0.1431017056382906
1106	2	-0.1657600909764092
1106	3	-0.06135461688479893
1106	4	-0.04301492865446477
1106	5	-0.0043516743450169315
1106	6	0.04148449887745187
1106	7	-0.08251833958812682
1106	8	-0.14559829188312534
1106	9	-0.07893673882903585
1106	10-11	-0.08893534167189898
1106	12-13	-0.07324630482381167
1106	14-15	-0.09792773365086305
1106	16-17	-0.05005103987736703
1106	18-19	-0.09174761136394238
1106	20-21	-0.10177265611156372
1106	22-23	-0.029707883527450463
1106	24-25	-0.10364061532715141
1106	26-27	-0.1702752806971901
1106	28-29	-0.13949113778834032
1106	30-31	-0.12601575532762865
1106	32-33	-0.1534066112257193
1106	34-35	-0.13015795094032256
1106	36-37	-0.19437581313907515
1106	38-39	-0.26295156778062534
1106	40-41	-0.26468357165286704
1106	42-43	-0.2401981226008587
1106	44-45	-0.2831806801823191
1106	46-47	-0.2564547602358118
1106	48-49	-0.2443603568486168
1106	50-51	-0.303568390634787
1106	52-53	-0.3649841260176032
1106	54-55	-0.36810127566461404
1106	56-57	-0.3032138782187772
1106	58-59	-0.3695369281535221
1106	60-61	-0.37397760977398775
1106	62-63	-0.3436573488459729
1106	64-65	-0.30253561323501543
1106	66-67	-0.209954656339427
1106	68-69	-0.19423120743692834
1106	70-71	-0.2437378040152538
1106	72-73	-0.26081242316516295
1106	74-75	-0.25580415778407684
1106	76-77	-0.24253703556373551
1106	78-79	-0.21051638696735608
1106	80-81	-0.17012587048737515
1106	82-83	-0.23657610220121228
1106	84-85	-0.25569192690732834
1106	86-87	-0.3166711588378881
1106	88-89	-0.35657805401234555
1106	90-91	-0.30946211573280635
1106	92-93	-0.2542512136823092
1106	94-95	-0.33696623551492166
1106	96-97	-0.32746405096368747
1106	98-99	-0.3313238336573825
1106	100	-0.2947406241342705
1107	1	-0.267556050895255
1107	2	-0.39011348392916645
1107	3	-0.31109979710174684
1107	4	-0.2557837458928276
1107	5	-0.15383271370058083
1107	6	-0.2636128090413976
1107	7	-0.21119375649626448
1107	8	-0.23899199134098126
1107	9	-0.26889710545192713
1107	10-11	-0.2618051289528154
1107	12-13	-0.2786380608347159
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2216	64-65	0.3086058170534969
2216	66-67	0.18144656922358848
2216	68-69	0.21227544744239424
2216	70-71	0.17804664198802556
2216	72-73	0.220018617106561
2216	74-75	0.2546742557642503
2216	76-77	0.2681332186924408
2216	78-79	0.2705068300001443
2216	80-81	0.23990922882762789
2216	82-83	0.27569071629616104
2216	84-85	0.2942273956711219
2216	86-87	0.24418274810031093
2216	88-89	0.26172719330601524
2216	90-91	0.2953623215025303
2216	92-93	0.31425633868796155
2216	94-95	0.2693883411480158
2216	96-97	0.202474464253239
2216	98-99	0.20282514789264638
2216	100	0.20246303232828566
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105336.0
3	20535.0
4	13763.0
5	11807.0
6	8274.0
7	7696.0
8	8423.0
9	9097.0
10	9513.0
11	10052.0
12	11455.0
13	12640.0
14	13749.0
15	14238.0
16	14475.0
17	14475.0
18	14394.0
19	13852.0
20	13366.0
21	14257.0
22	16401.0
23	23258.0
24	35848.0
25	48466.0
26	55724.0
27	46008.0
28	43415.0
29	52727.0
30	63131.0
31	72203.0
32	91181.0
33	121499.0
34	171052.0
35	267146.0
36	447935.0
37	826949.0
38	1272782.0
39	649758.0
40	2626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50469992146149	21.099388390736117	13.901662021510289	22.49424966629211
2	15.084599689309176	22.171646984553796	40.03430095641926	22.709452369717773
3	17.376748700239038	28.228564853153465	30.675112877238824	23.719573569368674
4	11.9492930883025	18.876654967579906	39.17652764870764	29.99752429540995
5	12.123220794042345	40.02704028869409	34.81995300920184	13.029785908061726
6	28.21666128921513	39.90746335028217	18.820377262484712	13.055498098017992
7	25.254006974416022	34.72347394021787	23.18908304359365	16.83343604177245
8	23.915922275560835	37.226334711815454	21.18103957567286	17.676703436950852
9	24.87304368261448	18.366802771098573	21.47868250795138	35.28147103833557
10-11	22.345341717043436	29.003426226468438	29.369057549347854	19.282174507140276
12-13	23.26433124103682	27.120831253628047	28.77764339958042	20.83719410575471
14-15	21.14979135097406	28.696464712718427	26.2300402727003	23.923703663607213
16-17	20.449287692324578	31.604602898129013	26.867697132626127	21.078412276920275
18-19	20.49081762977203	30.497907315806987	28.681933956152424	20.329341098268557
20-21	22.139024749317063	29.639377732932598	27.927310342333843	20.2942871754165
22-23	21.69556670465098	29.702866837996034	27.950957515647406	20.650608941705578
24-25	21.340538325431215	29.75245699571458	27.98324574606083	20.92375893279337
26-27	21.12673358301499	29.556518930760422	28.081889786150576	21.23485770007401
28-29	21.01498489887655	29.66220080915979	28.206638683089487	21.116175608874176
30-31	21.3061869777348	30.01411451211841	27.8666100579486	20.81308845219819
32-33	20.98914144341027	29.768172361976852	28.194742232638216	21.047943961974656
34-35	21.194670575327546	29.480385937214983	28.175332281912162	21.149611205545305
36-37	21.40854090037051	29.72177361851434	27.758760329021893	21.110925152093248
38-39	21.30587066115889	29.755012971940015	27.967332623444374	20.971783743456722
40-41	21.139616997375914	29.519988110201044	28.174820079080938	21.165574813342104
42-43	21.079506594294184	29.51369199219307	28.152838890864956	21.25396252264779
44-45	21.24944972031261	29.57346731634651	28.085198902562013	21.091884060778863
46-47	21.260246998828944	29.73706531141007	27.907847746297932	21.094839943463054
48-49	21.198993064700296	29.45702276453443	28.041344232148337	21.30263993861694
50-51	21.132314197531993	29.427527429199284	28.109716151389225	21.330442221879494
52-53	21.224338199421993	29.46126618036991	28.014257635109107	21.30013798509899
54-55	21.273432441554505	29.710273767375224	27.914148924079406	21.102144866990866
56-57	21.18843101771809	29.449268530760342	27.882562421201655	21.479738030319908
58-59	21.145551394441863	29.199200629921528	28.159261831155025	21.495986144481577
60-61	21.347512021075385	29.559468672665346	27.812449483931612	21.280569822327656
62-63	21.211647378592964	29.52297248157814	27.93735755094213	21.328022588886757
64-65	21.193003300116928	29.2788547684887	28.103447069794274	21.424694861600102
66-67	21.081111970504775	29.36153914284647	27.923540485710358	21.63380840093839
68-69	21.512610160597635	29.47985892297747	27.730300232592203	21.277230683832695
70-71	21.454553750507664	29.25522744916321	27.925005127745983	21.365213672583142
72-73	21.24171858680238	29.402220945725926	28.112447940867757	21.243612526603936
74-75	21.278551136944515	29.38565027922933	28.02410194523201	21.31169663859415
76-77	21.345350184102372	29.451117383519588	27.78081329631017	21.42271913606787
78-79	21.400852202470084	29.408841304678635	27.939860686828617	21.250445806022668
80-81	21.25446445147719	29.267891842558562	28.04704968166988	21.430594024294365
82-83	21.250015471420923	29.318627859858527	28.11638813624537	21.314968532475184
84-85	21.275992103874454	29.267703346166705	27.95204118676946	21.50426336318938
86-87	21.399071011303576	29.39178986490079	27.839116498557697	21.370022625237937
88-89	21.288522762251837	29.217070666411228	27.972578530791303	21.521828040545632
90-91	21.235502037458414	29.166285493007678	27.954874348300667	21.643338121233242
92-93	21.338019643113693	29.322520814242736	27.849055913408016	21.49040362923556
94-95	21.171182999173084	29.1833775489252	28.010832138443376	21.634607313458343
96-97	21.192892800122596	29.203698578069304	27.947763105390234	21.655645516417867
98-99	21.185446337820984	29.26130260842837	27.87816744034271	21.675083613407935
100	21.250870483843713	29.3053458202245	27.77312204210397	21.670661653827814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18044.0
1	13142.5
2	7474.0
3	5706.0
4	3957.5
5	2879.5
6	2472.5
7	2513.5
8	2838.0
9	2801.5
10	2164.5
11	1714.0
12	1629.0
13	1636.5
14	1738.5
15	1919.5
16	2158.5
17	2533.0
18	3260.5
19	4347.5
20	6044.5
21	8599.0
22	12584.5
23	18377.0
24	25373.5
25	33719.0
26	44420.5
27	56953.5
28	72504.5
29	88876.0
30	101165.5
31	112419.5
32	122949.5
33	132772.5
34	141730.0
35	147913.0
36	152278.5
37	155968.0
38	157782.0
39	157659.5
40	156901.5
41	154669.5
42	151217.0
43	147236.0
44	143997.0
45	141548.0
46	140159.5
47	137410.0
48	133027.0
49	130165.5
50	127506.0
51	123402.0
52	117601.5
53	111830.0
54	107482.0
55	103138.5
56	95924.0
57	87280.5
58	80377.0
59	74283.0
60	66615.0
61	56450.5
62	47583.5
63	39441.0
64	31182.5
65	24992.0
66	20205.0
67	15843.0
68	12294.5
69	9771.5
70	7566.5
71	5810.5
72	4585.0
73	3592.0
74	2832.0
75	2283.5
76	1717.0
77	1301.0
78	988.5
79	703.5
80	498.0
81	361.0
82	267.0
83	212.0
84	177.5
85	153.5
86	131.0
87	109.0
88	107.5
89	103.5
90	78.5
91	60.5
92	47.5
93	34.0
94	36.5
95	32.0
96	26.5
97	25.0
98	24.5
99	28.0
100	48.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18219139839802334
2	0.17713709800568062
3	0.19023526370328375
4	0.18072887743343058
5	0.19406362740471783
6	0.0766317970124138
7	0.07891160910427904
8	0.06648018090524026
9	0.060845173659309186
10-11	0.07382504722007026
12-13	0.07344866314830005
14-15	0.07111508190332479
16-17	0.0672974720325127
18-19	0.07140543533011894
20-21	0.07308303290715186
22-23	0.07074945166217658
24-25	0.0706096518640905
26-27	0.07434123108992655
28-29	0.07158825045069304
30-31	0.07696516576169597
32-33	0.07412615447748643
34-35	0.07341640165643404
36-37	0.08427777058465996
38-39	0.07977191555403951
40-41	0.07541661415212712
42-43	0.07814808713011662
44-45	0.08906322521145257
46-47	0.07946005446600134
48-49	0.08440681655212402
50-51	0.08113765204303425
52-53	0.07989020769088157
54-55	0.0819441893396847
56-57	0.07631993592437562
58-59	0.08276148046695714
60-61	0.07972890023155148
62-63	0.07156674278944902
64-65	0.07438424641241456
66-67	0.07453480004112265
68-69	0.07294323310906578
70-71	0.06914713089949771
72-73	0.06682430348514444
74-75	0.071512973636339
76-77	0.07711571939040406
78-79	0.0737067550832282
80-81	0.08126669801049831
82-83	0.08266469599135907
84-85	0.08466490848705217
86-87	0.08192268167844068
88-89	0.08336369498178946
90-91	0.08101935990619219
92-93	0.07201840367557329
94-95	0.07166352726504709
96-97	0.07071719017031057
98-99	0.08000849982772364
100	0.0882029187616921
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4649506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.05088720835671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03346724455096	47.93977567296467
2	14.890130155766428	18.7767183393796
3	4.4563872148576875	8.429375029222648
4	1.723973233515272	4.347921675863896
5	0.881436794601446	2.778768595885562
6	0.5017571169968394	1.8981738833854778
7	0.3138073363670932	1.3850081679305513
8	0.21920894501731825	1.105705477387984
9	0.14886839988205997	0.8447656220867076
>10	0.7751653046707394	8.489112397477767
>50	0.04041133310749604	1.7516144580736748
>100	0.01490947448997234	1.5449220426801447
>500	2.3872350530002585E-4	0.09020282129801176
>1k	2.046194326314387E-4	0.21459389823079073
>5k	0.0	0.0
>10k+	3.410323877121812E-5	0.40334191813259584
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18758	0.40344070961517203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.301532248802346E-5	0.0
3	0.0	0.0	0.0	4.301532248802346E-5	0.0
4	0.0	0.0	0.0	4.301532248802346E-5	0.0
5	0.0	0.0	0.0	4.301532248802346E-5	0.0
6	0.0	0.0	0.0	6.452298373203519E-5	0.0
7	0.0	0.0	0.0	6.452298373203519E-5	0.0
8	0.0	0.0	0.0	6.452298373203519E-5	0.0
9	0.0	0.0	0.0	6.452298373203519E-5	0.0
10-11	0.0	0.0	0.0	1.1829213684206452E-4	0.0
12-13	0.0	0.0	0.0	1.5055362870808212E-4	0.0
14-15	2.150766124401173E-5	0.0	0.0	2.1507661244011728E-4	0.0
16-17	2.150766124401173E-5	0.0	0.0	2.473381043061349E-4	0.0
18-19	2.150766124401173E-5	0.0	0.0	2.6884576555014664E-4	0.0
20-21	2.150766124401173E-5	0.0	0.0	3.2261491866017596E-4	0.0
22-23	2.150766124401173E-5	2.150766124401173E-5	0.0	4.624147167462522E-4	0.0
24-25	2.150766124401173E-5	3.2261491866017596E-5	0.0	7.635219741624164E-4	0.0
26-27	2.150766124401173E-5	4.301532248802346E-5	0.0	0.001204429029664657	0.0
28-29	2.150766124401173E-5	4.301532248802346E-5	0.0	0.002613180841147425	0.0
30-31	2.150766124401173E-5	4.301532248802346E-5	0.0	0.005968375995213255	0.0
32-33	2.150766124401173E-5	4.301532248802346E-5	0.0	0.012538966505258837	0.0
34-35	2.150766124401173E-5	4.301532248802346E-5	0.0	0.02204535277511202	0.0
36-37	2.150766124401173E-5	5.376915311002933E-5	0.0	0.03873529790046512	0.0
38-39	2.150766124401173E-5	6.452298373203519E-5	0.0	0.06136135752916547	0.0
40-41	2.150766124401173E-5	6.452298373203519E-5	0.0	0.08662210566025724	0.0
42-43	2.150766124401173E-5	6.452298373203519E-5	0.0	0.1107752092372824	0.0
44-45	2.150766124401173E-5	6.452298373203519E-5	0.0	0.14164945695306125	0.0
46-47	2.150766124401173E-5	8.603064497604692E-5	0.0	0.18271833609850163	0.0
48-49	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.2260777811664293	0.0
50-51	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.2718030689711982	0.0
52-53	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.31584000536831225	0.0
54-55	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.3677057304582465	0.0
56-57	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.43000267125152647	0.0
58-59	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.5019242904515018	2.150766124401173E-5
60-61	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.5785130721414276	2.150766124401173E-5
62-63	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.6551663768150853	2.150766124401173E-5
64-65	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.7393151014322812	2.150766124401173E-5
66-67	2.150766124401173E-5	1.0753830622005864E-4	0.0	0.839788140933682	2.150766124401173E-5
68-69	2.150766124401173E-5	1.2904596746407039E-4	0.0	0.9530152235527818	2.150766124401173E-5
70-71	2.150766124401173E-5	1.2904596746407039E-4	0.0	1.0819536527106322	2.150766124401173E-5
72-73	2.150766124401173E-5	1.2904596746407039E-4	0.0	1.2179573485871402	2.150766124401173E-5
74-75	2.150766124401173E-5	1.2904596746407039E-4	0.0	1.355251504138289	2.150766124401173E-5
76-77	2.150766124401173E-5	1.2904596746407039E-4	0.0	1.5068912697391936	2.150766124401173E-5
78-79	2.150766124401173E-5	1.2904596746407039E-4	0.0	1.6848779203640127	2.150766124401173E-5
80-81	2.150766124401173E-5	1.2904596746407039E-4	0.0	1.884705600982126	2.150766124401173E-5
82-83	2.150766124401173E-5	1.2904596746407039E-4	0.0	2.101094180758128	2.150766124401173E-5
84-85	2.150766124401173E-5	1.2904596746407039E-4	0.0	2.316289085335087	2.150766124401173E-5
86-87	2.150766124401173E-5	1.2904596746407039E-4	0.0	2.550432239468021	2.150766124401173E-5
88	2.150766124401173E-5	1.2904596746407039E-4	0.0	2.742850530787572	2.150766124401173E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5400	0.0	47.585117	1
GTATCAA	9530	0.0	36.148514	1
TCAACGC	10475	0.0	31.355772	4
ATCAACG	10485	0.0	31.327215	3
CAACGCA	11330	0.0	29.029528	5
TATCAAC	11630	0.0	29.01177	2
AACGCAG	11860	0.0	27.77598	6
GTACATG	20330	0.0	26.899302	1
TACATGG	20800	0.0	25.293755	2
CGCAGAG	13455	0.0	24.099396	8
ACGCAGA	13575	0.0	24.059713	7
ACATGGG	21285	0.0	23.987825	3
CATGGGT	9400	0.0	22.777163	4
GTGGTAT	3195	0.0	20.327337	1
GCAGAGT	15440	0.0	20.118687	9
GTACCTG	3660	0.0	19.673544	1
TACCTGG	3370	0.0	19.40974	2
CATGGGG	10125	0.0	18.218271	4
ATGGGTG	5210	0.0	17.70154	5
TGGTATC	3165	0.0	17.693268	2
>>END_MODULE