FastQCFastQC Report
Wed 25 May 2016
SRR2431409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4649506
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287380.618087168830409No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232910.5009349380342771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146190.3144204997262075No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59860.1287448602066542No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49340.10611880057795388No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48240.10375295784111258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113600.064.413671
GTATCAA227450.045.714041
ATCAACG289750.035.5576483
TCAACGC292150.035.2969784
TATCAAC293050.035.2871252
CAACGCA300250.034.3760725
AACGCAG310900.033.2565966
GTGGTAT38300.033.1521571
TGGTATC41600.029.5039372
ACGCAGA353250.029.1232227
CGCAGAG353400.028.9512758
GCAGAGT382200.026.536079
TATAACG3550.025.1685412
GTACATG203100.024.4512371
TACATGG204600.023.4437642
ACATGGG201150.023.1201343
GAGTACT268350.023.0653512-13
CAGAGTA377950.022.5009110-11
CATGGGT90600.022.4212744
TACCTGG36750.022.1371422
Produced by FastQC (version 0.11.5)