##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431409_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4649506
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.634572576097334	34.0	31.0	34.0	31.0	34.0
2	32.76115935757476	34.0	31.0	34.0	31.0	34.0
3	32.869801436969865	34.0	31.0	34.0	31.0	34.0
4	36.33463770129558	37.0	37.0	37.0	35.0	37.0
5	36.22924736520396	37.0	37.0	37.0	35.0	37.0
6	36.26952669810513	37.0	37.0	37.0	35.0	37.0
7	36.23068730312425	37.0	37.0	37.0	35.0	37.0
8	36.24432143974005	37.0	37.0	37.0	35.0	37.0
9	38.045612372583236	39.0	39.0	39.0	35.0	39.0
10-11	38.02489834403913	39.0	39.0	39.0	35.0	39.0
12-13	38.00514151395869	39.0	39.0	39.0	35.0	39.0
14-15	39.483627185339685	41.0	39.0	41.0	36.0	41.0
16-17	39.50916710291373	41.0	39.0	41.0	36.5	41.0
18-19	39.572569429956644	41.0	40.0	41.0	37.0	41.0
20-21	39.56441329465969	41.0	40.0	41.0	37.0	41.0
22-23	39.50633669469401	41.0	39.5	41.0	36.0	41.0
24-25	39.45597682850608	41.0	39.0	41.0	36.0	41.0
26-27	39.34067124550436	41.0	39.0	41.0	36.0	41.0
28-29	39.262999015379265	41.0	39.0	41.0	36.0	41.0
30-31	39.09703525492816	41.0	39.0	41.0	35.0	41.0
32-33	38.888694304298134	41.0	38.0	41.0	35.0	41.0
34-35	38.757229155097335	41.0	38.0	41.0	35.0	41.0
36-37	38.620597327974195	41.0	38.0	41.0	35.0	41.0
38-39	38.47460719482887	41.0	38.0	41.0	35.0	41.0
40-41	38.33952467208344	41.0	38.0	41.0	34.5	41.0
42-43	38.172956223736456	40.0	38.0	41.0	34.0	41.0
44-45	37.943096965570106	40.0	38.0	41.0	33.5	41.0
46-47	37.60387910027431	40.0	38.0	41.0	33.0	41.0
48-49	37.29139794636247	40.0	38.0	41.0	32.5	41.0
50-51	37.12715211035323	40.0	37.0	41.0	32.0	41.0
52-53	37.069107986956034	40.0	37.0	41.0	32.0	41.0
54-55	36.92586975906688	40.0	37.0	41.0	32.0	41.0
56-57	36.77549980578582	40.0	36.0	41.0	31.0	41.0
58-59	36.59527872423436	40.0	36.0	41.0	31.0	41.0
60-61	36.43822322199391	40.0	35.0	41.0	31.0	41.0
62-63	36.27601781780688	39.0	35.0	41.0	31.0	41.0
64-65	36.04047279431406	39.0	35.0	41.0	31.0	41.0
66-67	35.771975775490986	39.0	35.0	41.0	30.5	41.0
68-69	35.47873494517482	38.0	35.0	41.0	30.0	41.0
70-71	35.173576719763346	37.0	35.0	40.0	29.5	41.0
72-73	34.852419267767374	37.0	35.0	39.5	29.0	41.0
74-75	34.51228603640902	36.0	35.0	39.0	29.0	41.0
76-77	34.17741443929742	36.0	35.0	39.0	29.0	41.0
78-79	33.855466903365645	35.0	35.0	38.0	29.0	40.0
80-81	33.591257651888185	35.0	34.5	37.0	29.0	39.0
82-83	33.390706023392596	35.0	35.0	37.0	29.0	39.0
84-85	33.130046288788535	35.0	34.0	36.5	29.0	38.5
86-87	32.887637955516134	35.0	34.0	36.0	29.0	37.0
88-89	32.654498671471764	35.0	34.0	36.0	29.0	37.0
90-91	32.47667849014498	35.0	34.0	35.5	29.0	36.5
92-93	32.319702996404345	35.0	34.0	35.0	29.0	36.0
94-95	32.183102893081546	35.0	34.0	35.0	29.0	36.0
96-97	32.04238159924947	35.0	34.0	35.0	27.5	36.0
98-99	31.927991920001823	35.0	34.0	35.0	27.0	36.0
100	31.827784285040174	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.011592620170581824
1101	2	-0.0035149767407602894
1101	3	0.02820216578243162
1101	4	0.05675590300914024
1101	5	0.012429157394812762
1101	6	0.018486159422216986
1101	7	0.04636096034906245
1101	8	0.023747212406533436
1101	9	0.04302914029756266
1101	10-11	0.05372290969390292
1101	12-13	0.03275671909867839
1101	14-15	0.043899759444123276
1101	16-17	0.03634178941080535
1101	18-19	0.0177263685572413
1101	20-21	0.06620013728873175
1101	22-23	0.041653058055466374
1101	24-25	0.02415338343926976
1101	26-27	0.04666967742275574
1101	28-29	0.06921740062893633
1101	30-31	0.025284153759429273
1101	32-33	0.035493153034913405
1101	34-35	-0.015786087100124746
1101	36-37	-0.0210606231954813
1101	38-39	-0.02060791482522717
1101	40-41	-0.03560528335485458
1101	42-43	-0.06822714111571315
1101	44-45	-0.09897544086320664
1101	46-47	-0.07940986390308069
1101	48-49	-0.08561435798537786
1101	50-51	-0.1249980358838485
1101	52-53	-0.047499998337848126
1101	54-55	-0.051330557962209866
1101	56-57	-0.028297106153182483
1101	58-59	-0.06610218398228795
1101	60-61	-0.030065819032635943
1101	62-63	-0.024787792117621166
1101	64-65	-0.036006844111383884
1101	66-67	-0.023612206812558156
1101	68-69	-0.08242103072097251
1101	70-71	-0.04686252702894933
1101	72-73	-0.07781093898253033
1101	74-75	-0.09810055743454882
1101	76-77	-0.11453629217013628
1101	78-79	-0.04715882259500148
1101	80-81	-0.013937763822802651
1101	82-83	-0.1454271286740223
1101	84-85	-0.11219025432352936
1101	86-87	-0.07809855034766855
1101	88-89	-0.06357553394333593
1101	90-91	-0.11343368151543132
1101	92-93	-0.1153866462727251
1101	94-95	-0.13551905773160655
1101	96-97	-0.11136537572348715
1101	98-99	-0.07808348953797406
1101	100	-0.044079875543918234
1102	1	-0.05784809785858158
1102	2	-0.011063108389080867
1102	3	-0.00895983566601899
1102	4	0.012950194686418115
1102	5	0.0026838044408066253
1102	6	-0.032527450905242006
1102	7	-0.012400866341607752
1102	8	0.02210229341658021
1102	9	0.020410338689885066
1102	10-11	0.032466453798356554
1102	12-13	0.021985491946686864
1102	14-15	0.046838093392075564
1102	16-17	0.04891044900876551
1102	18-19	0.04127499799120926
1102	20-21	0.08537040319684763
1102	22-23	0.07893080037680278
1102	24-25	0.11437698548171227
1102	26-27	0.15350164279696799
1102	28-29	0.09261104290144573
1102	30-31	0.05989338631324159
1102	32-33	0.05626814021921689
1102	34-35	0.058962822281195315
1102	36-37	0.010097980523376293
1102	38-39	0.07056021456251216
1102	40-41	0.014960633855167771
1102	42-43	-0.04563388527406431
1102	44-45	-0.048702515036701755
1102	46-47	-0.11815784355171388
1102	48-49	-0.12072660903071153
1102	50-51	-0.08288667308907094
1102	52-53	-0.03429678571753669
1102	54-55	-0.05934098710444147
1102	56-57	-0.043882497133743925
1102	58-59	-0.00324499518490029
1102	60-61	-0.09883521145632557
1102	62-63	-0.08058949722376951
1102	64-65	-0.0823020515544215
1102	66-67	-0.04281743759595713
1102	68-69	-0.10798944066495864
1102	70-71	-0.12281632125547759
1102	72-73	-0.017436969899286225
1102	74-75	-0.023711191627924677
1102	76-77	-0.09120734680971765
1102	78-79	-0.04399205146184926
1102	80-81	-0.09432938564879834
1102	82-83	-0.10465380267807944
1102	84-85	-0.08518581078738663
1102	86-87	-0.0766276945940021
1102	88-89	-0.06619514045671337
1102	90-91	-0.0710650533525623
1102	92-93	-0.03495730043842116
1102	94-95	-0.05423818636314337
1102	96-97	-0.07261363794539832
1102	98-99	-0.010874386834672123
1102	100	-0.01014967513952314
1103	1	-0.0972429529767922
1103	2	-0.10387119976557102
1103	3	-0.058819788523010175
1103	4	-0.020581870946301706
1103	5	-0.03718502426927017
1103	6	-0.045532872464605134
1103	7	-0.08862035654571088
1103	8	-0.05497858732012162
1103	9	-0.06061570309343978
1103	10-11	-0.04328741477144149
1103	12-13	-0.037655931940598464
1103	14-15	-0.09877520224102909
1103	16-17	-0.026103606956191072
1103	18-19	-0.03905004880822105
1103	20-21	-0.08916966019450712
1103	22-23	-0.04030924361523347
1103	24-25	-0.03819880568593703
1103	26-27	-0.09889636556486892
1103	28-29	-0.08436238230848403
1103	30-31	-0.13269516246959512
1103	32-33	-0.12285638860699777
1103	34-35	-0.10622461462830302
1103	36-37	-0.11037886500299265
1103	38-39	-0.12415813085394944
1103	40-41	-0.17250830730262834
1103	42-43	-0.05244870301346083
1103	44-45	-0.10474794664877862
1103	46-47	-0.09808965551270887
1103	48-49	-0.16269073509515408
1103	50-51	-0.17005230531517412
1103	52-53	-0.13462415398154093
1103	54-55	-0.07724089306899629
1103	56-57	-0.0789377035732528
1103	58-59	-0.1573073398906928
1103	60-61	-0.15078638059751626
1103	62-63	-0.1095493406321566
1103	64-65	-0.15181696036813008
1103	66-67	-0.13549403309508534
1103	68-69	-0.10691687847798903
1103	70-71	-0.0882920335730546
1103	72-73	-0.13320150809487785
1103	74-75	-0.12787092205711303
1103	76-77	-0.11831291327554538
1103	78-79	-0.0756634338209139
1103	80-81	-0.08905820897566485
1103	82-83	-0.11032388023360085
1103	84-85	-0.1127097344003758
1103	86-87	-0.09100962315970662
1103	88-89	-0.09623309820567982
1103	90-91	-0.07008384070147144
1103	92-93	-0.11229206170341399
1103	94-95	-0.13165841283026936
1103	96-97	-0.045593441773100096
1103	98-99	-0.015659726339848135
1103	100	0.04385313339602348
1104	1	-0.1628890024230003
1104	2	-0.12175525292928313
1104	3	-0.09775854916692595
1104	4	-0.01800459063173321
1104	5	-0.05875714830430212
1104	6	-0.09816360173507377
1104	7	-0.09956103958666773
1104	8	-0.09664907508771847
1104	9	-0.08874560287141975
1104	10-11	-0.11722581072669414
1104	12-13	-0.10960628689648644
1104	14-15	-0.14620817734324731
1104	16-17	-0.13544619873578512
1104	18-19	-0.07691283853245068
1104	20-21	-0.07348275168118334
1104	22-23	-0.11375143070656435
1104	24-25	-0.06131458481190322
1104	26-27	-0.08390749902186201
1104	28-29	-0.05364880274294137
1104	30-31	-0.07341118800430735
1104	32-33	-0.0897692665765959
1104	34-35	-0.07463996342165302
1104	36-37	-0.03167403471329777
1104	38-39	-0.07070987181257493
1104	40-41	-0.18649984327370817
1104	42-43	-0.1550668307808607
1104	44-45	-0.0823919045939121
1104	46-47	-0.08982061888077908
1104	48-49	-0.060410339224453935
1104	50-51	-0.19017397892486088
1104	52-53	-0.13568964881294932
1104	54-55	-0.06638449492002962
1104	56-57	-0.15343399187892004
1104	58-59	-0.15095921367031906
1104	60-61	-0.10166330584005578
1104	62-63	-0.13669031858312763
1104	64-65	-0.10221664287993804
1104	66-67	-0.09099327096546972
1104	68-69	-0.11051564708466088
1104	70-71	-0.14350989207478193
1104	72-73	-0.11446309625127782
1104	74-75	-0.14093597461641139
1104	76-77	-0.13355202127960553
1104	78-79	-0.11956986957694227
1104	80-81	-0.11206904139030627
1104	82-83	-0.09004080285595961
1104	84-85	-0.09343397588281022
1104	86-87	-0.11114944990239195
1104	88-89	-0.10005378102363949
1104	90-91	-0.0864792788876727
1104	92-93	-0.11883840898643427
1104	94-95	-0.11249955212693408
1104	96-97	-0.16389390202412812
1104	98-99	-0.13381644815580174
1104	100	-0.07626083994035682
1105	1	-0.2048815479442041
1105	2	-0.18276497950787274
1105	3	-0.16184002340665415
1105	4	-0.07575790486494327
1105	5	-0.12285480333437704
1105	6	-0.053141288085726046
1105	7	-0.04426573450521687
1105	8	-0.03599577850627611
1105	9	-0.04632228623938062
1105	10-11	-0.08479243484806886
1105	12-13	-0.13023130669815686
1105	14-15	-0.16548821137948266
1105	16-17	-0.20737258406036574
1105	18-19	-0.25714426909536314
1105	20-21	-0.18158774351158513
1105	22-23	-0.23696973333033355
1105	24-25	-0.22075822587444804
1105	26-27	-0.13903986467605023
1105	28-29	-0.1329670935988787
1105	30-31	-0.13100631521020745
1105	32-33	-0.1476963828655542
1105	34-35	-0.12167961427866913
1105	36-37	-0.10278990953961653
1105	38-39	-0.030189721636737943
1105	40-41	-0.06432344776540333
1105	42-43	-0.18608846098982923
1105	44-45	-0.08474157034177665
1105	46-47	-0.05675641848338131
1105	48-49	0.019183025067370352
1105	50-51	0.05321978909465486
1105	52-53	0.06912843610565034
1105	54-55	0.09178884094792039
1105	56-57	0.036252168679787644
1105	58-59	-0.029742463497193228
1105	60-61	-0.010624295425508024
1105	62-63	-0.012288668368810818
1105	64-65	-0.028410853098961297
1105	66-67	-0.0706917538183589
1105	68-69	-0.011002315471245083
1105	70-71	0.03218718316249891
1105	72-73	0.046640942836084776
1105	74-75	-0.010265229937971299
1105	76-77	-0.05752572919257659
1105	78-79	-0.0058929716720754755
1105	80-81	0.04350829599840722
1105	82-83	-0.09161944828618829
1105	84-85	-0.06293488336489617
1105	86-87	-0.035964841794346114
1105	88-89	-0.021890097053713475
1105	90-91	-0.007981738820419082
1105	92-93	-0.013213773902862158
1105	94-95	-0.02126461836634519
1105	96-97	-0.05937974439833482
1105	98-99	-0.1077267907359456
1105	100	-0.11497818982109465
1106	1	-0.12094613579348845
1106	2	-0.10852635623147222
1106	3	-0.0771584568477337
1106	4	-0.004647162955386364
1106	5	-0.03496811303107705
1106	6	-0.06890177756580584
1106	7	-0.06087771681602305
1106	8	-0.06130709660222067
1106	9	-0.08324275094622635
1106	10-11	-0.09813766478331587
1106	12-13	-0.061267911582518764
1106	14-15	-0.09503863986591199
1106	16-17	-0.12692356055015352
1106	18-19	-0.08436146457077598
1106	20-21	-0.06643971301824081
1106	22-23	-0.09218063633548468
1106	24-25	-0.07082150721415559
1106	26-27	-0.12037321772589848
1106	28-29	-0.09001880945826457
1106	30-31	-0.11942387288973322
1106	32-33	-0.12850321777215612
1106	34-35	-0.12107537534225088
1106	36-37	-0.08333348537457397
1106	38-39	-0.06846057968821384
1106	40-41	-0.19788586969667676
1106	42-43	-0.17912794854580483
1106	44-45	-0.1286679099925152
1106	46-47	-0.18406094655681215
1106	48-49	-0.21098042780607784
1106	50-51	-0.1951205001119405
1106	52-53	-0.16238206120304
1106	54-55	-0.14583219820561766
1106	56-57	-0.1472963357059811
1106	58-59	-0.11437314403038101
1106	60-61	-0.11539566494678155
1106	62-63	-0.19324215941114176
1106	64-65	-0.1916154723323089
1106	66-67	-0.13838699958481016
1106	68-69	-0.1792055951408429
1106	70-71	-0.12915757357620095
1106	72-73	-0.11090284524144778
1106	74-75	-0.10605604227030341
1106	76-77	-0.19136911157365688
1106	78-79	-0.08553712612899034
1106	80-81	-0.09476351888431367
1106	82-83	-0.08669346353286045
1106	84-85	-0.07417878139104062
1106	86-87	-0.0771716060694061
1106	88-89	-0.1021603670888851
1106	90-91	-0.10552276948425288
1106	92-93	-0.0364835185506962
1106	94-95	-0.03912678769415834
1106	96-97	-0.10634332740083607
1106	98-99	-0.09788021427817029
1106	100	-0.04944499125599222
1107	1	-0.2812082882707898
1107	2	-0.21446298776523776
1107	3	-0.21672029774676815
1107	4	-0.20545161382730726
1107	5	-0.24735678062706512
1107	6	-0.2452338365256992
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2216	22-23	-0.10494743007054552
2216	24-25	-0.0763384122116193
2216	26-27	-0.04398969671083108
2216	28-29	-0.04005839555607338
2216	30-31	-0.07611543200610527
2216	32-33	-0.04442365804805348
2216	34-35	-0.02425242344234846
2216	36-37	-0.061440369115430826
2216	38-39	-0.056150275953108064
2216	40-41	-0.005792314126594533
2216	42-43	0.004999064433469869
2216	44-45	-0.0035513977783807604
2216	46-47	0.012238825099068151
2216	48-49	0.05268397448114115
2216	50-51	0.05342067389842953
2216	52-53	-0.02921425344298001
2216	54-55	-0.02622223416103253
2216	56-57	-0.01326813845660979
2216	58-59	-0.03029206396089279
2216	60-61	-0.05754275179368307
2216	62-63	-0.02698638638253925
2216	64-65	-0.03165757464373087
2216	66-67	-0.0352168409172009
2216	68-69	-0.01754951366188351
2216	70-71	-0.004705981473883014
2216	72-73	0.005553737692764571
2216	74-75	0.008058923189793177
2216	76-77	0.05775157441692613
2216	78-79	0.0323466327319295
2216	80-81	0.033432625089737655
2216	82-83	0.021657066222665833
2216	84-85	0.017590500469033543
2216	86-87	0.0017164193678382844
2216	88-89	0.03012135650499914
2216	90-91	0.016512011045414
2216	92-93	-0.07284122594914777
2216	94-95	-0.0640308177820117
2216	96-97	-0.01959024452679614
2216	98-99	-0.0506675600588693
2216	100	-0.010523527964050317
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39.0
3	240.0
4	72.0
5	119.0
6	343.0
7	832.0
8	1500.0
9	2417.0
10	3950.0
11	5720.0
12	8804.0
13	12988.0
14	14725.0
15	19111.0
16	24392.0
17	36262.0
18	42158.0
19	21874.0
20	13337.0
21	11748.0
22	11748.0
23	11860.0
24	13147.0
25	15527.0
26	18730.0
27	23796.0
28	30363.0
29	38418.0
30	48790.0
31	61949.0
32	79089.0
33	102555.0
34	143827.0
35	231836.0
36	380431.0
37	801268.0
38	1470849.0
39	935518.0
40	9174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.576963563397435	20.819680523166653	14.405257993481065	23.19809791995485
2	14.415713076923408	21.850723360249365	40.238432925226604	23.49513063760062
3	16.454215700085925	27.929708041677912	31.833425996324312	23.782650261911854
4	11.290382247060226	18.40877288899079	39.492840744801704	30.80800411914728
5	11.574083239014147	38.63737105527033	35.62823034251852	14.160315363197013
6	27.904018190319572	39.64638393842271	18.49215809163382	13.9574397796239
7	24.415583074847092	34.65131564514596	23.671955687335387	17.261145592671564
8	24.760802545474725	35.04486283058888	21.376356972117037	18.817977651819355
9	25.576765698714166	15.84280477169075	22.236496143137817	36.343933386457266
10-11	22.76570887315771	27.31354685852648	29.746396713973482	20.174347554342333
12-13	24.44970760869086	25.53879116822208	29.772037047810784	20.23946417527627
14-15	21.755063871301598	26.69178187962334	28.36462626352133	23.188527985553733
16-17	19.68025205258365	29.962376648185852	28.86745387574508	21.48991742348542
18-19	19.537425728604912	27.720818272580587	32.71699850865748	20.02475749015701
20-21	21.06408723851523	26.923043007149577	32.47752556938307	19.535344184952123
22-23	20.571613660295807	26.94265882984508	32.56210770009016	19.923619809768955
24-25	20.36707133340001	27.053256206843525	32.521752315569074	20.057920144187392
26-27	20.137672956881143	26.896683662770403	32.60123621974592	20.364407160602536
28-29	20.067465340616845	27.000288310664043	32.696666102806574	20.235580245912544
30-31	20.340269321808925	27.313405212847382	32.342318577281596	20.004006888062108
32-33	20.05655957262755	27.153477175931524	32.610904897241255	20.17905835419967
34-35	20.244264925389185	27.02848287758148	32.415968736154305	20.311283460875025
36-37	20.54968513973769	27.2548209264184	31.884342717574693	20.311151216269216
38-39	20.440828295463636	27.344910755073933	31.95794902280957	20.256311926652863
40-41	20.389665764956323	27.215930323653254	31.952216341658428	20.442187569732
42-43	20.372820266528787	27.25922624879046	31.745503098304134	20.622450386376624
44-45	20.66106255092618	27.376097147660733	31.430748621437644	20.532091679975448
46-47	20.789228694732113	27.720573915289627	30.77304093782957	20.71715645214869
48-49	20.850512592531416	27.695611271916864	30.42518317015457	21.028692965397155
50-51	20.862971418284594	27.79271548610617	30.244003997424667	21.100309098184567
52-53	20.890579927716583	27.945901860768824	30.106070017352494	21.057448194162095
54-55	20.96724153153387	28.158739400269212	29.96913948999988	20.90487957819704
56-57	20.79101924084289	27.887107741600996	30.036736946963018	21.285136070593097
58-59	20.690745764797025	27.60085962264183	30.364113679760457	21.344280932800686
60-61	20.905569962882687	27.869698496434253	30.084047620711925	21.14068391997114
62-63	20.794031637508915	27.765201020034734	30.262507421577546	21.178259920878805
64-65	20.725017281673423	27.598047418244885	30.39450608396635	21.282429216115336
66-67	20.70614641738049	27.700893582068915	30.164099753757885	21.42886024679271
68-69	21.149848198469854	27.755591363529607	29.908009305102578	21.18655113289796
70-71	21.092056938693517	27.588487086331153	29.98552314962581	21.33393282534952
72-73	20.972094818899688	27.775801458007805	30.027626656228257	21.22447706686425
74-75	21.01195187418808	27.809615268804254	29.87538391390005	21.303048943107616
76-77	21.05446823364763	27.87491008386652	29.667103024505042	21.403518657980804
78-79	21.114682248089217	27.918621235954173	29.717127882466563	21.249568633490043
80-81	20.977601012243642	27.745453329285564	29.84015286892161	21.436792789549184
82-83	20.89493396836349	27.75223586162457	29.959943545865116	21.392886624146833
84-85	20.88073007352806	27.778503670165684	29.747156088048705	21.593610168257555
86-87	20.980136843110483	27.86528705135187	29.61670206899281	21.537874036544835
88-89	20.786687527940163	27.68321032759935	29.774825788328048	21.755276356132434
90-91	20.719992455024	27.681620079271717	29.697696599265527	21.90069086643876
92-93	20.76134506502966	27.818171396629133	29.64011592325166	21.780367615089553
94-95	20.58425540010068	27.645345220270638	29.763369981568882	22.007029398059803
96-97	20.61668619566508	27.65889033994222	29.68864797388792	22.03577549050478
98-99	20.53922234945237	27.640030351702936	29.69253415211812	22.128213146726566
100	20.59303593785051	27.66427092467382	29.54283891662306	22.199854220852615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3594.0
1	2067.0
2	2737.0
3	4751.5
4	2812.5
5	1477.5
6	3483.0
7	8299.5
8	14519.5
9	16139.0
10	10475.0
11	5270.5
12	4361.0
13	4665.5
14	5193.5
15	5830.0
16	6433.0
17	7083.0
18	8166.5
19	9371.5
20	10963.5
21	13174.5
22	16688.5
23	22215.0
24	28699.0
25	36233.0
26	46354.0
27	58329.5
28	72864.5
29	88795.5
30	101157.5
31	111566.5
32	121447.5
33	130889.5
34	139885.0
35	146111.5
36	150312.5
37	154150.0
38	155772.5
39	155526.5
40	155492.0
41	153544.5
42	149716.0
43	146803.5
44	144454.5
45	141650.0
46	139802.0
47	136925.0
48	132441.5
49	129376.5
50	126460.5
51	121906.0
52	115469.5
53	109319.0
54	104809.0
55	100059.5
56	92865.5
57	84006.0
58	77528.5
59	72395.0
60	64023.5
61	53554.0
62	45727.0
63	37657.0
64	28970.5
65	23240.0
66	18456.0
67	14052.5
68	10763.5
69	8196.0
70	6143.0
71	4566.5
72	3475.0
73	2698.5
74	2191.0
75	1668.0
76	1072.5
77	760.0
78	555.5
79	317.5
80	146.0
81	65.5
82	36.5
83	23.0
84	14.5
85	11.5
86	9.0
87	5.0
88	4.5
89	4.5
90	2.0
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006022145148323285
2	0.004989777408610722
3	8.81814111004481E-4
4	0.0
5	0.046585594254529406
6	0.0
7	0.0
8	0.0
9	2.150766124401173E-5
10-11	0.0
12-13	1.0753830622005865E-5
14-15	0.0
16-17	0.0
18-19	8.603064497604692E-5
20-21	0.0
22-23	4.301532248802346E-5
24-25	2.150766124401173E-5
26-27	1.9356895119610555E-4
28-29	1.6130745933008798E-4
30-31	2.6884576555014664E-4
32-33	0.0015485516095688444
34-35	9.893524172245394E-4
36-37	0.001344228827750733
38-39	0.0011614137071766335
40-41	1.8281512057409969E-4
42-43	5.807068535883167E-4
44-45	1.3979979808607625E-4
46-47	9.678447559805278E-5
48-49	8.603064497604692E-5
50-51	2.6884576555014664E-4
52-53	6.452298373203519E-4
54-55	7.85029635406428E-4
56-57	0.002140012293779167
58-59	5.161838698562815E-4
60-61	0.001258198182774686
62-63	0.0012151828602866629
64-65	0.0015485516095688444
66-67	3.6563024114819937E-4
68-69	3.011072574161642E-4
70-71	3.011072574161642E-4
72-73	2.3658427368412904E-4
74-75	5.59199192344305E-4
76-77	7.420143129184047E-4
78-79	8.065372966504399E-4
80-81	6.989989904303812E-4
82-83	0.0014625209645927975
84-85	0.0012151828602866629
86-87	0.0020217201569371025
88-89	0.0013227211665067214
90-91	0.0011721675377986392
92-93	0.0010861368928225925
94-95	6.989989904303812E-4
96-97	2.1507661244011728E-4
98-99	3.011072574161642E-4
100	1.505536287080821E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4649506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.56177581333487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2345974647834	42.88748078512468
2	16.449124751733336	19.265799120731707
3	4.9989982005300115	8.78250635732109
4	2.017325374620456	4.725526253242998
5	0.9819085274722343	2.875115352751538
6	0.5650871505069648	1.9855504213791053
7	0.36094797615479873	1.4796428121897987
8	0.24546604662231106	1.1499942073665108
9	0.18188468668295435	0.9586341220865244
>10	0.8921754144986885	9.180368299757708
>50	0.049597653278687155	1.985834809006051
>100	0.02152521057714604	2.134315329291232
>500	5.887754320841202E-4	0.2332984712854998
>1k	6.255733721973034E-4	0.7906843070005904
>5k	3.6798433658571044E-5	0.1289971999045995
>10k+	1.1039530097571315E-4	1.4362521515605993
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28738	0.618087168830409	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23291	0.5009349380342771	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14619	0.3144204997262075	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5986	0.1287448602066542	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4934	0.10611880057795388	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4824	0.10375295784111258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.150766124401173E-5	0.0
10-11	0.0	0.0	0.0	4.301532248802346E-5	0.0
12-13	2.150766124401173E-5	4.301532248802346E-5	0.0	4.301532248802346E-5	0.0
14-15	6.452298373203519E-5	4.301532248802346E-5	0.0	5.376915311002933E-5	0.0
16-17	6.452298373203519E-5	4.301532248802346E-5	0.0	9.678447559805279E-5	0.0
18-19	6.452298373203519E-5	4.301532248802346E-5	0.0	1.0753830622005864E-4	0.0
20-21	6.452298373203519E-5	4.301532248802346E-5	0.0	1.3979979808607625E-4	0.0
22-23	6.452298373203519E-5	4.301532248802346E-5	0.0	2.903534267941583E-4	0.0
24-25	6.452298373203519E-5	4.301532248802346E-5	0.0	6.667374985643637E-4	0.0
26-27	6.452298373203519E-5	4.301532248802346E-5	0.0	0.001107644554066604	0.0
28-29	6.452298373203519E-5	4.301532248802346E-5	0.0	0.0025271501961713783	0.0
30-31	6.452298373203519E-5	4.301532248802346E-5	0.0	0.005925360672725232	0.0
32-33	6.452298373203519E-5	4.301532248802346E-5	0.0	0.012603489488990873	0.0
34-35	6.452298373203519E-5	4.301532248802346E-5	0.0	0.022228167895686123	0.0
36-37	8.603064497604692E-5	4.301532248802346E-5	0.0	0.03907942048036931	0.0
38-39	8.603064497604692E-5	4.301532248802346E-5	0.0	0.06202809502772983	0.0
40-41	8.603064497604692E-5	5.376915311002933E-5	0.0	0.08766522723059181	0.0
42-43	8.603064497604692E-5	6.452298373203519E-5	0.0	0.1120118997588131	0.0
44-45	8.603064497604692E-5	6.452298373203519E-5	0.0	0.1433700698525822	0.0
46-47	8.603064497604692E-5	6.452298373203519E-5	0.0	0.18485834839228082	0.0
48-49	8.603064497604692E-5	6.452298373203519E-5	0.0	0.22861568519322267	0.0
50-51	8.603064497604692E-5	6.452298373203519E-5	0.0	0.27498620283531194	0.0
52-53	8.603064497604692E-5	6.452298373203519E-5	0.0	0.31960384608601433	0.0
54-55	8.603064497604692E-5	6.452298373203519E-5	0.0	0.372319123795087	0.0
56-57	8.603064497604692E-5	6.452298373203519E-5	0.0	0.43542260188501747	0.0
58-59	8.603064497604692E-5	6.452298373203519E-5	0.0	0.5081937737041311	0.0
60-61	8.603064497604692E-5	6.452298373203519E-5	0.0	0.5856213541825734	0.0
62-63	8.603064497604692E-5	6.452298373203519E-5	0.0	0.6632962727653218	0.0
64-65	8.603064497604692E-5	6.452298373203519E-5	0.0	0.7485849034284502	0.0
66-67	8.603064497604692E-5	6.452298373203519E-5	0.0	0.8507355405068839	0.0
68-69	8.603064497604692E-5	6.452298373203519E-5	0.0	0.9653498672762224	2.150766124401173E-5
70-71	8.603064497604692E-5	7.527681435404106E-5	0.0	1.096180970623546	2.150766124401173E-5
72-73	8.603064497604692E-5	8.603064497604692E-5	0.0	1.2338730179077089	2.150766124401173E-5
74-75	8.603064497604692E-5	8.603064497604692E-5	0.0	1.3725651714397185	2.150766124401173E-5
76-77	8.603064497604692E-5	8.603064497604692E-5	0.0	1.5262911801812924	2.150766124401173E-5
78-79	8.603064497604692E-5	8.603064497604692E-5	0.0	1.7076975489439095	2.150766124401173E-5
80-81	8.603064497604692E-5	8.603064497604692E-5	0.0	1.910654594273026	2.150766124401173E-5
82-83	8.603064497604692E-5	8.603064497604692E-5	0.0	2.1306887226298876	2.150766124401173E-5
84-85	8.603064497604692E-5	8.603064497604692E-5	0.0	2.3497334985695257	2.150766124401173E-5
86-87	8.603064497604692E-5	8.603064497604692E-5	0.0	2.5884470307168117	2.150766124401173E-5
88	8.603064497604692E-5	8.603064497604692E-5	0.0	2.7848119778746385	2.150766124401173E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11360	0.0	64.41367	1
GTATCAA	22745	0.0	45.71404	1
ATCAACG	28975	0.0	35.557648	3
TCAACGC	29215	0.0	35.296978	4
TATCAAC	29305	0.0	35.287125	2
CAACGCA	30025	0.0	34.376072	5
AACGCAG	31090	0.0	33.256596	6
GTGGTAT	3830	0.0	33.152157	1
TGGTATC	4160	0.0	29.503937	2
ACGCAGA	35325	0.0	29.123222	7
CGCAGAG	35340	0.0	28.951275	8
GCAGAGT	38220	0.0	26.53607	9
TATAACG	355	0.0	25.168541	2
GTACATG	20310	0.0	24.451237	1
TACATGG	20460	0.0	23.443764	2
ACATGGG	20115	0.0	23.120134	3
GAGTACT	26835	0.0	23.06535	12-13
CAGAGTA	37795	0.0	22.50091	10-11
CATGGGT	9060	0.0	22.421274	4
TACCTGG	3675	0.0	22.137142	2
>>END_MODULE