FastQCFastQC Report
Wed 25 May 2016
SRR2431408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268090
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196850.60233959285087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41050.047.110381
GTATCAA76500.041.947961
ATCAACG89600.034.81453
TCAACGC90250.034.509514
TATCAAC98450.032.2110942
CAACGCA97800.031.9892545
AACGCAG103150.030.2520336
GTACATG171450.027.5265241
CGCAGAG112850.027.0682938
ACGCAGA115300.026.819197
TACATGG169550.026.7232842
TACCTGG25450.025.6973482
ACATGGG172550.025.0857723
CATGGGT79000.024.5356834
TCTGTCG5800.023.4973588
GCAGAGT127800.023.2763719
GTACCTG28950.023.2421021
CTGTCGC6250.021.8052169
GTGGTAT19950.021.6986561
CATGGGG75150.020.5339684
Produced by FastQC (version 0.11.5)