FastQCFastQC Report
Wed 25 May 2016
SRR2431408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268090
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260590.7973770612192442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202610.6199645664593081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136480.4176139579999327No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54930.16807982644296823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41280.126312310860472No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39850.12193666637087718No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39400.12055971530771797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38780.1186625827318097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89950.065.290721
GTATCAA179550.045.436441
ATCAACG230450.035.2149963
TATCAAC232950.034.8376052
TCAACGC233500.034.834494
CAACGCA241650.033.6401945
AACGCAG249600.032.5562636
GTGGTAT29050.031.2393051
ATAACGC3600.030.039243
TATAACG3150.029.8531172
TGGTATC29550.029.277272
ACGCAGA278950.028.9286427
CGCAGAG279850.028.7516388
GTACATG163350.027.1445581
TACATGG165200.026.611652
GCAGAGT301350.026.4819939
CATGGGT78350.026.2236274
ACATGGG165150.026.0499023
GAGTACT213350.022.99775112-13
CAGAGTA299150.022.5759110-11
Produced by FastQC (version 0.11.5)