##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431408_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3268090
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6311787619068	34.0	31.0	34.0	31.0	34.0
2	32.73068948529569	34.0	31.0	34.0	31.0	34.0
3	32.83301347270118	34.0	31.0	34.0	31.0	34.0
4	36.307181258778066	37.0	37.0	37.0	35.0	37.0
5	36.21597477425652	37.0	37.0	37.0	35.0	37.0
6	36.2617284101723	37.0	37.0	37.0	35.0	37.0
7	36.21277657592049	37.0	37.0	37.0	35.0	37.0
8	36.225069077045	37.0	37.0	37.0	35.0	37.0
9	38.01665560005998	39.0	39.0	39.0	35.0	39.0
10-11	37.990740616078504	39.0	38.5	39.0	35.0	39.0
12-13	37.970587254328976	39.0	38.0	39.0	35.0	39.0
14-15	39.420571495889035	41.0	39.0	41.0	36.0	41.0
16-17	39.45476746356435	41.0	39.0	41.0	36.0	41.0
18-19	39.534603392195436	41.0	39.5	41.0	36.5	41.0
20-21	39.535242909467	41.0	40.0	41.0	36.0	41.0
22-23	39.471498948927355	41.0	39.0	41.0	36.0	41.0
24-25	39.423486501289744	41.0	39.0	41.0	36.0	41.0
26-27	39.307186460593186	41.0	39.0	41.0	36.0	41.0
28-29	39.224792768865	41.0	39.0	41.0	35.0	41.0
30-31	39.042428452092814	41.0	39.0	41.0	35.0	41.0
32-33	38.8103630866959	41.0	38.0	41.0	35.0	41.0
34-35	38.67596899106206	41.0	38.0	41.0	35.0	41.0
36-37	38.534004418482965	41.0	38.0	41.0	35.0	41.0
38-39	38.39025042150002	40.5	38.0	41.0	35.0	41.0
40-41	38.2503711648088	40.0	38.0	41.0	34.0	41.0
42-43	38.08029384135688	40.0	38.0	41.0	34.0	41.0
44-45	37.834163991811735	40.0	38.0	41.0	33.0	41.0
46-47	37.47181947253594	40.0	38.0	41.0	33.0	41.0
48-49	37.138005226294254	40.0	37.0	41.0	32.0	41.0
50-51	36.972990186928754	40.0	37.0	41.0	31.0	41.0
52-53	36.9173786523627	40.0	37.0	41.0	31.0	41.0
54-55	36.77583037798837	40.0	36.5	41.0	31.0	41.0
56-57	36.633595464017205	40.0	36.0	41.0	31.0	41.0
58-59	36.457976371519756	40.0	36.0	41.0	31.0	41.0
60-61	36.30581226343216	40.0	35.0	41.0	31.0	41.0
62-63	36.15159022548339	39.0	35.0	41.0	31.0	41.0
64-65	35.920909001894074	39.0	35.0	41.0	30.0	41.0
66-67	35.65999528776747	39.0	35.0	41.0	30.0	41.0
68-69	35.370931033111084	38.0	35.0	41.0	29.5	41.0
70-71	35.07043793163591	37.0	35.0	40.0	29.0	41.0
72-73	34.75569675253742	37.0	35.0	39.5	29.0	41.0
74-75	34.41923998421096	36.0	35.0	39.0	29.0	41.0
76-77	34.08648583729334	36.0	35.0	39.0	29.0	41.0
78-79	33.76587043196485	35.5	34.0	38.5	28.5	40.0
80-81	33.49506806728089	35.0	34.0	37.0	29.0	39.0
82-83	33.29087616926094	35.0	34.0	37.0	29.0	39.0
84-85	33.022620552065575	35.0	34.0	36.5	29.0	38.5
86-87	32.775581914818744	35.0	34.0	36.0	29.0	37.0
88-89	32.53860251706654	35.0	34.0	36.0	28.0	37.0
90-91	32.35325817220456	35.0	34.0	35.5	28.0	37.0
92-93	32.18678004583717	35.0	34.0	35.0	27.0	36.0
94-95	32.04807196252245	35.0	34.0	35.0	27.0	36.0
96-97	31.90493024977892	35.0	34.0	35.0	27.0	36.0
98-99	31.789510386800853	35.0	34.0	35.0	26.5	36.0
100	31.68682166035819	35.0	34.0	35.0	26.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.015766006546307665
1101	2	0.009543115899248278
1101	3	-0.019795561430406394
1101	4	0.020252065085571758
1101	5	0.00884343341238747
1101	6	0.006543069364504106
1101	7	0.01963993879656556
1101	8	-0.009288900346120954
1101	9	0.00784868642491432
1101	10-11	-0.0018610926176307885
1101	12-13	0.05010701353293712
1101	14-15	0.051893705546078195
1101	16-17	0.005640792494659763
1101	18-19	-0.0328634978794895
1101	20-21	0.04256685310953401
1101	22-23	0.004983381073429882
1101	24-25	0.030481265106892863
1101	26-27	0.05235758820263925
1101	28-29	0.00431344708875514
1101	30-31	-0.08843601940282042
1101	32-33	-0.07535745596248233
1101	34-35	-0.014577218788183188
1101	36-37	-0.02387883749084807
1101	38-39	0.009807112573390953
1101	40-41	-0.022570460543484216
1101	42-43	-0.05227143718233407
1101	44-45	0.0022977891956372787
1101	46-47	-0.029613067725712483
1101	48-49	0.0422861366031384
1101	50-51	-0.02948067335790938
1101	52-53	-0.021643825982948783
1101	54-55	-0.017506836561665295
1101	56-57	0.029346718273771444
1101	58-59	0.06391272945752036
1101	60-61	0.02234380140271952
1101	62-63	0.007766583793056725
1101	64-65	0.04042078871769661
1101	66-67	0.051363355615343664
1101	68-69	0.0626619663095127
1101	70-71	0.086786293995182
1101	72-73	0.011122702389123162
1101	74-75	0.00423088949042949
1101	76-77	0.029541270709273704
1101	78-79	0.04592512438272678
1101	80-81	0.055326922751604
1101	82-83	0.06228529711428621
1101	84-85	0.09249296282640529
1101	86-87	0.11016738115851865
1101	88-89	0.0979185106951519
1101	90-91	0.06847693162949753
1101	92-93	0.021861160739270247
1101	94-95	-0.042095786183232065
1101	96-97	-0.03055540009710711
1101	98-99	-0.04323596386752371
1101	100	0.003454238005623722
1102	1	-0.06169270591796305
1102	2	-0.04070719537745049
1102	3	-0.020337959105120262
1102	4	-0.04197625518794723
1102	5	-0.07086969868525017
1102	6	-0.05858601938115271
1102	7	-0.09310713072166976
1102	8	-0.025437225109442352
1102	9	-0.07735576952767786
1102	10-11	-0.0658411618566177
1102	12-13	-0.07501076470045831
1102	14-15	-0.13358801410439014
1102	16-17	-0.14867143648189085
1102	18-19	-0.10185757739886725
1102	20-21	-0.04859431422223537
1102	22-23	-0.05937651137033839
1102	24-25	-0.06621055715286417
1102	26-27	-0.010438203870407392
1102	28-29	-0.12170231521216834
1102	30-31	-0.12099212275709448
1102	32-33	-0.12621778358262503
1102	34-35	-0.1004338735469048
1102	36-37	-0.09179973089006666
1102	38-39	-0.17723157897368225
1102	40-41	-0.2249780970175621
1102	42-43	-0.152792630475318
1102	44-45	-0.21262672365801905
1102	46-47	-0.33145511069660216
1102	48-49	-0.3047219862365367
1102	50-51	-0.37814074652547447
1102	52-53	-0.3648833169148489
1102	54-55	-0.35724706138694984
1102	56-57	-0.2928542015030615
1102	58-59	-0.3209079882870398
1102	60-61	-0.3655643829455073
1102	62-63	-0.360030093115995
1102	64-65	-0.40258878063647785
1102	66-67	-0.4101775081927812
1102	68-69	-0.3742556995881827
1102	70-71	-0.392702777714554
1102	72-73	-0.4227247410232451
1102	74-75	-0.37566746978376386
1102	76-77	-0.3913409945070754
1102	78-79	-0.36392525007715193
1102	80-81	-0.3588054016423996
1102	82-83	-0.36694011157358375
1102	84-85	-0.3198401120714536
1102	86-87	-0.2789432296770329
1102	88-89	-0.3522497665540598
1102	90-91	-0.33437325862156086
1102	92-93	-0.2972839971711423
1102	94-95	-0.3143795773003859
1102	96-97	-0.3448158559845247
1102	98-99	-0.2965905332646912
1102	100	-0.3118361805557477
1103	1	-0.04352491629114752
1103	2	-0.049424766507499385
1103	3	-0.056906807009362126
1103	4	0.02634672701795182
1103	5	-0.009502338943093491
1103	6	-0.027005822943543478
1103	7	-0.03314830277365388
1103	8	0.057250835238754405
1103	9	0.014064593284807358
1103	10-11	0.044737098424171506
1103	12-13	0.027723735841085784
1103	14-15	-0.03560761245523736
1103	16-17	0.013101183617003187
1103	18-19	0.033392153855650974
1103	20-21	-0.0053029097800703084
1103	22-23	0.027771629359577332
1103	24-25	-0.009347133920549311
1103	26-27	-0.03369830490302661
1103	28-29	0.013115882553698555
1103	30-31	-0.0010806161794860714
1103	32-33	-0.034917974441249555
1103	34-35	0.010876358351332271
1103	36-37	0.0364867419338637
1103	38-39	-0.036769050217174026
1103	40-41	-0.0023728375798413026
1103	42-43	-0.0023676652696522638
1103	44-45	0.05636953458306948
1103	46-47	0.0869600572257454
1103	48-49	0.1482622847529811
1103	50-51	0.03440425499766775
1103	52-53	0.08566798637574635
1103	54-55	0.06945711747361116
1103	56-57	0.06430145626183048
1103	58-59	0.01896166508331021
1103	60-61	-0.006109893713293957
1103	62-63	-0.038713474035589
1103	64-65	-0.025372762243122793
1103	66-67	-0.01979931294611248
1103	68-69	0.05539136737613859
1103	70-71	0.015564055132379906
1103	72-73	-0.00680876422900667
1103	74-75	0.014953387028484144
1103	76-77	0.03390234956526683
1103	78-79	0.027947026822197074
1103	80-81	0.07750908071879081
1103	82-83	0.05487995423358427
1103	84-85	0.047973598448905364
1103	86-87	0.09331684351472092
1103	88-89	0.12495619669004299
1103	90-91	0.13905580676042462
1103	92-93	0.12394681526598816
1103	94-95	0.10600668368966382
1103	96-97	0.16912167558986368
1103	98-99	0.10096871440471844
1103	100	0.18002401394651812
1104	1	-0.11153655882064584
1104	2	-0.0910054100114337
1104	3	-0.0424864152645128
1104	4	-0.01242931672393155
1104	5	-0.03173602800776365
1104	6	-0.0628877049713168
1104	7	-0.08112554827091145
1104	8	-0.1273629980896871
1104	9	-0.09603229789313872
1104	10-11	-0.029643971691747595
1104	12-13	-0.0768210570182859
1104	14-15	-0.11414678187254879
1104	16-17	-0.08635701463069267
1104	18-19	-0.0796933104404971
1104	20-21	-0.03625248404187431
1104	22-23	-0.051125438355896335
1104	24-25	0.009460854166739807
1104	26-27	-0.030905238972849247
1104	28-29	-0.05822827283427756
1104	30-31	-0.08672491000840665
1104	32-33	9.212673595939691E-4
1104	34-35	0.015973001377133755
1104	36-37	0.11169324558205318
1104	38-39	0.07933074171948817
1104	40-41	0.03448391537386897
1104	42-43	0.014081828954346065
1104	44-45	0.07114872024561691
1104	46-47	0.03770054670758327
1104	48-49	0.10139543270617679
1104	50-51	-0.04690353649022683
1104	52-53	-0.005032800511195035
1104	54-55	0.13156512010052523
1104	56-57	0.09198245616116196
1104	58-59	0.10613895371011495
1104	60-61	0.07151539428299003
1104	62-63	0.041146362820114746
1104	64-65	0.056829447324517446
1104	66-67	-0.009503729669752659
1104	68-69	0.04282191535897084
1104	70-71	0.043815848643227184
1104	72-73	0.058048134994656664
1104	74-75	0.05459794141713559
1104	76-77	-0.03277495094487648
1104	78-79	0.020140741547898244
1104	80-81	-0.023433093541328276
1104	82-83	-0.01914396029303589
1104	84-85	0.0318844927822326
1104	86-87	-0.009330066047454011
1104	88-89	0.045207132677980155
1104	90-91	0.03496793276719501
1104	92-93	0.06187216900512027
1104	94-95	-0.019503185950078716
1104	96-97	-0.009217294466914439
1104	98-99	-0.029657785867204467
1104	100	0.026031848913461886
1105	1	-0.13360398735937906
1105	2	-0.16083444725774854
1105	3	-0.11602372741626965
1105	4	-0.0454398661459976
1105	5	-0.12599223067083187
1105	6	0.018268155466351743
1105	7	-0.017884858470253562
1105	8	-0.04285231698943903
1105	9	-0.0011500636934869135
1105	10-11	-0.05371565771027775
1105	12-13	-0.08566611694909199
1105	14-15	-0.1044616918074226
1105	16-17	-0.11493811839724088
1105	18-19	-0.14291719576051776
1105	20-21	-0.02698056875851762
1105	22-23	-0.1004108041179066
1105	24-25	-0.11737809462078275
1105	26-27	-0.006582045894020894
1105	28-29	-0.004683315936482302
1105	30-31	-0.04643931312013905
1105	32-33	-0.11517688218270195
1105	34-35	-0.11580678788261167
1105	36-37	-0.14696711587112787
1105	38-39	-0.04651708741737792
1105	40-41	-0.01440794546641655
1105	42-43	-0.052359575803720304
1105	44-45	-0.0745184857156147
1105	46-47	-0.09939704199594246
1105	48-49	-0.04546400806828643
1105	50-51	-0.09600334200636951
1105	52-53	-0.14201771301758725
1105	54-55	-0.13372732353638384
1105	56-57	-0.12913290375460917
1105	58-59	-0.11904225029731208
1105	60-61	-0.10500173577369054
1105	62-63	-0.13676894311450383
1105	64-65	-0.12148972629489663
1105	66-67	-0.1484888708894303
1105	68-69	-0.05315383810223295
1105	70-71	-0.04313645886187345
1105	72-73	-0.07283845303048508
1105	74-75	-0.09421385684498773
1105	76-77	-0.12106590768686942
1105	78-79	-3.270081546205006E-4
1105	80-81	0.007504213853465558
1105	82-83	-0.06072457940111775
1105	84-85	-0.09812808989581612
1105	86-87	-0.09986467948579758
1105	88-89	-0.04682226085780172
1105	90-91	-0.14350963385438575
1105	92-93	-0.13505764376366614
1105	94-95	-0.07681458710939637
1105	96-97	-0.1283107134186494
1105	98-99	-0.1969127817100329
1105	100	-0.18546016663145792
1106	1	-0.1417223347861878
1106	2	-0.12228329768226587
1106	3	-0.09472828732231875
1106	4	-0.07752473494066692
1106	5	-0.048794933210707825
1106	6	-0.11801472186869688
1106	7	-0.10684459244770039
1106	8	-0.12472290874064385
1106	9	-0.16647858878205568
1106	10-11	-0.13174039555992323
1106	12-13	-0.12223460320433333
1106	14-15	-0.19357711982704018
1106	16-17	-0.21985304864789157
1106	18-19	-0.15596863312374154
1106	20-21	-0.15453183085667632
1106	22-23	-0.18507998721644725
1106	24-25	-0.1559082234752438
1106	26-27	-0.12329501570873447
1106	28-29	-0.19576736219417512
1106	30-31	-0.28746765446714306
1106	32-33	-0.2749331040709606
1106	34-35	-0.2929887397900188
1106	36-37	-0.3142099123194626
1106	38-39	-0.3333691721932226
1106	40-41	-0.3191412021103659
1106	42-43	-0.29432438387622284
1106	44-45	-0.3147282554560036
1106	46-47	-0.3328286951682031
1106	48-49	-0.2874895758313514
1106	50-51	-0.29934413151309514
1106	52-53	-0.3145947199559984
1106	54-55	-0.33180406400624207
1106	56-57	-0.3252365319012114
1106	58-59	-0.2457588234814665
1106	60-61	-0.3411520546512108
1106	62-63	-0.3728170151583612
1106	64-65	-0.35164035617675893
1106	66-67	-0.30526716440683543
1106	68-69	-0.34444673140578885
1106	70-71	-0.30306157563453695
1106	72-73	-0.25299536994137384
1106	74-75	-0.29438271335485666
1106	76-77	-0.3187485629356601
1106	78-79	-0.2500391457960873
1106	80-81	-0.23985379490693504
1106	82-83	-0.27381853575622017
1106	84-85	-0.18849322749476727
1106	86-87	-0.1943299236423215
1106	88-89	-0.19834102500654183
1106	90-91	-0.25905273445508215
1106	92-93	-0.20475625736396807
1106	94-95	-0.1869553624090159
1106	96-97	-0.22539452968492313
1106	98-99	-0.27272053702576926
1106	100	-0.1868064734591961
1107	1	-0.23888869663444723
1107	2	-0.19305046023139738
1107	3	-0.2132244380707462
1107	4	-0.21887007583175233
1107	5	-0.24305953226161847
1107	6	-0.21563043873045729
1107	7	-0.13082823149229483
1107	8	-0.13522715197765933
1107	9	-0.1382802548122939
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2216	40-41	0.021919659072104025
2216	42-43	0.06334461625797161
2216	44-45	0.05756866328929533
2216	46-47	0.07110957377395977
2216	48-49	0.12303509913912336
2216	50-51	0.12490627015146316
2216	52-53	0.06214986964155145
2216	54-55	0.05222090153741732
2216	56-57	0.10670443322369749
2216	58-59	0.09968040463316186
2216	60-61	-0.014240172181281707
2216	62-63	-0.004052803847486075
2216	64-65	0.00940269867247423
2216	66-67	0.010701317114801157
2216	68-69	-0.044218219783395796
2216	70-71	0.027586220740374756
2216	72-73	0.022996245749311583
2216	74-75	0.009705704314100672
2216	76-77	0.04387338378370487
2216	78-79	0.04834347892347779
2216	80-81	0.09138037019869216
2216	82-83	0.11013469566272249
2216	84-85	0.1480470566971448
2216	86-87	0.13557217909824715
2216	88-89	0.18624950602694668
2216	90-91	0.1754010277653535
2216	92-93	0.17660070546352102
2216	94-95	0.12160695853359726
2216	96-97	0.14729189398219233
2216	98-99	0.11213740269226591
2216	100	0.18646537098647542
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41.0
3	181.0
4	68.0
5	102.0
6	254.0
7	584.0
8	1166.0
9	1966.0
10	2984.0
11	4241.0
12	6666.0
13	9758.0
14	11070.0
15	13770.0
16	18064.0
17	27454.0
18	31830.0
19	16296.0
20	9848.0
21	8901.0
22	8911.0
23	9014.0
24	9385.0
25	11090.0
26	13750.0
27	17200.0
28	21791.0
29	27772.0
30	34966.0
31	44390.0
32	56733.0
33	72609.0
34	102500.0
35	168272.0
36	267897.0
37	551768.0
38	1014362.0
39	663809.0
40	6627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83469383008907	20.955903503263844	14.707716580924156	22.501686085722923
2	14.20864470631004	22.04344722130506	40.91603861662422	22.831869455760685
3	16.110771460272176	28.940668812280794	32.08072551867131	22.86783420877572
4	10.823967516194474	18.725861282890005	40.090450385393304	30.359720815522216
5	11.02522328589656	39.24245040200033	35.69705975270184	14.035266559401274
6	26.862785296610557	40.476241474378	18.77209623969964	13.888876989311798
7	23.809350415686225	35.40483891202506	23.964150314097836	16.821660358190872
8	24.730561275852256	35.23149607262958	21.419299958079492	18.618642693438677
9	25.816923366436306	15.86494843310379	22.4442142722726	35.8739139281873
10-11	22.544008885924192	27.671407458178937	29.873075710889236	19.911507945007635
12-13	24.3519609313085	26.014506944423193	29.97547497161951	19.658057152648794
14-15	21.558956693199498	26.932723231723	28.883312406223883	22.625007668853623
16-17	19.08128878987078	30.30888999656986	29.322074766017693	21.287746447541668
18-19	19.032991303632077	27.81026278817113	33.57731897407307	19.579426934123724
20-21	20.705503826394008	26.839729016030773	33.376941883485465	19.077825274089758
22-23	20.134142654665357	26.961223198396127	33.55477269889917	19.349861448039345
24-25	19.822789430950408	27.100588891460898	33.443897420801974	19.632724256786723
26-27	19.521163225024797	27.082606289643802	33.52934507700454	19.86688540832686
28-29	19.553399213882496	27.05529673097698	33.668815069613366	19.72248898552716
30-31	19.830806088398266	27.38261510680033	33.2890566324096	19.49752217239181
32-33	19.50586182264152	27.228409816424364	33.5927640107483	19.672964350185822
34-35	19.761972444261396	27.066259657467246	33.346608254971514	19.82515964329985
36-37	20.007209171069253	27.288364853822277	32.822245127421944	19.882180847686527
38-39	19.89186269867437	27.4400850043642	32.86526364587023	19.802788651091202
40-41	19.80741635816555	27.2958656388073	32.88415101619862	20.01256698682853
42-43	19.79397036204969	27.359115393212548	32.69149240370512	20.155421841032645
44-45	20.1652130369374	27.50766619029165	32.28115193507143	20.045968837699526
46-47	20.283499358186827	27.837540945232455	31.618287162053623	20.2606725345271
48-49	20.384910252528506	27.888567208976873	31.185251675559993	20.541270862934624
50-51	20.38687853418521	27.96788940295219	30.9247325646863	20.7204994981763
52-53	20.353312775822406	28.129926936943622	30.873979806108654	20.642780481125314
54-55	20.472420224199947	28.424832431423486	30.722950736903947	20.379796607472617
56-57	20.343985306076238	28.120871445761843	30.691604313624392	20.843538934537527
58-59	20.245015909219912	27.861613271549736	31.066666646267286	20.826704172963062
60-61	20.44461185582657	28.05035335905294	30.80386430620698	20.70117047891351
62-63	20.335880609510333	27.936599199648963	31.00553966780353	20.72198052303717
64-65	20.338349022374384	27.85045373450278	31.075148739514187	20.736048503608657
66-67	20.261306212834658	27.911620319138308	30.884648990546896	20.942424477480138
68-69	20.69996966112718	28.02886522671002	30.600983542500202	20.670181569662596
70-71	20.62023144124245	27.842576994213587	30.66178021909763	20.875411345446334
72-73	20.469828589245907	28.017382042177292	30.748858926732485	20.763930441844316
74-75	20.540837847327673	28.08498268477415	30.534465599423516	20.83971386847466
76-77	20.615689381812206	28.039046821578378	30.42063121839406	20.924632578215352
78-79	20.633719014232547	28.095210847493306	30.54852201893899	20.722548119335155
80-81	20.513966408650234	28.013813030826878	30.57310369549522	20.89911686502767
82-83	20.372604116273514	28.025570576731774	30.703862513857636	20.897962793137076
84-85	20.375503949359583	28.06073263921135	30.473217838770783	21.090545572658286
86-87	20.509021086814332	28.054772933297123	30.417031628309037	21.019174351579505
88-89	20.296197086926636	27.939644936193808	30.542580224570504	21.221577752309052
90-91	20.216816605195465	27.950101306233066	30.422514998570254	21.410567090001216
92-93	20.270019274414235	28.079431149277113	30.369319685697615	21.281229890611044
94-95	20.15294023329949	27.867376932101294	30.546355437483975	21.433327397115242
96-97	20.130749253194722	27.991172206986505	30.40824335333739	21.46983518648139
98-99	20.047382524767965	27.899463982319904	30.444151884170612	21.60900160874152
100	20.094611982246484	27.959676691395725	30.29921192380247	21.64649940255532
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2993.0
1	1719.5
2	2457.0
3	4270.0
4	2514.0
5	1314.0
6	3047.0
7	7934.0
8	14250.0
9	15399.5
10	9637.0
11	4627.5
12	3721.5
13	3903.0
14	4362.0
15	4827.5
16	5317.5
17	5932.0
18	6575.0
19	7376.0
20	8709.0
21	10771.0
22	13926.5
23	18528.0
24	23632.5
25	28987.0
26	36081.5
27	45253.0
28	57008.0
29	68557.5
30	76118.0
31	83369.5
32	90995.5
33	97698.5
34	103564.0
35	107629.5
36	109860.0
37	111043.0
38	111250.5
39	110608.5
40	110153.0
41	107876.0
42	104061.5
43	101267.0
44	99341.5
45	96905.0
46	94707.5
47	92555.5
48	89594.5
49	87065.0
50	86108.0
51	83081.0
52	76949.5
53	71818.0
54	68318.0
55	65831.5
56	61012.5
57	54423.0
58	49762.0
59	45781.0
60	39823.0
61	32997.0
62	27961.5
63	22829.5
64	17492.5
65	13856.0
66	10978.0
67	8197.0
68	6113.5
69	4669.5
70	3363.5
71	2411.5
72	1841.5
73	1358.5
74	1032.0
75	772.5
76	503.5
77	384.0
78	295.0
79	159.0
80	80.0
81	39.5
82	15.5
83	9.0
84	8.5
85	6.0
86	2.5
87	1.0
88	1.0
89	1.0
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	1.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005079419477431772
2	0.005232414040005019
3	8.567695504101784E-4
4	0.0
5	0.0436340492458898
6	0.0
7	0.0
8	0.0
9	1.5299456257324615E-4
10-11	0.0
12-13	0.0
14-15	1.5299456257324616E-5
16-17	3.059891251464923E-5
18-19	4.589836877197384E-5
20-21	0.0
22-23	6.119782502929846E-5
24-25	1.5299456257324616E-5
26-27	1.070961938012723E-4
28-29	1.070961938012723E-4
30-31	1.6829401883057077E-4
32-33	0.0011168603067846967
34-35	7.037749878369323E-4
36-37	9.332668316968015E-4
38-39	6.884755315796076E-4
40-41	1.070961938012723E-4
42-43	5.048820564917122E-4
44-45	1.9889293134522E-4
46-47	1.5299456257324615E-4
48-49	1.070961938012723E-4
50-51	3.059891251464923E-4
52-53	6.884755315796076E-4
54-55	7.649728128662308E-4
56-57	0.002218421157312069
58-59	5.048820564917122E-4
60-61	7.9557172538088E-4
62-63	7.343739003515815E-4
64-65	0.0013616516069018907
66-67	2.6009075637451845E-4
68-69	1.6829401883057077E-4
70-71	1.070961938012723E-4
72-73	1.3769510631592152E-4
74-75	3.824864064331154E-4
76-77	4.130853189477646E-4
78-79	5.201815127490369E-4
80-81	5.048820564917122E-4
82-83	0.0011321597630420215
84-85	8.720690066675031E-4
86-87	0.0016064429070190846
88-89	9.026679191821523E-4
90-91	8.720690066675031E-4
92-93	8.261706378955292E-4
94-95	4.895826002343877E-4
96-97	7.649728128662307E-5
98-99	2.2949184385986923E-4
100	1.5299456257324615E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3268090.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.48484944656897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20484763016734	49.648229777930396
2	14.206775119707165	18.038299591917426
3	3.7724102889823077	7.184726977401885
4	1.3926651272007973	3.536525437193178
5	0.6827114459855544	2.1670916681921164
6	0.39805255832380665	1.5162184042205085
7	0.2780525391610848	1.2356486510814386
8	0.17930021190453577	0.9106277566797892
9	0.14103550693960687	0.8058256132213327
>10	0.6834841753758395	7.684799415364623
>50	0.03889634395516748	1.6929907404038376
>100	0.019793340262249978	2.2730761781508244
>500	0.0012528905602947015	0.5455496636005365
>1k	5.300690810417057E-4	0.7577979406142938
>5k	4.81880982765187E-5	0.16804263403957514
>10k+	1.4456429482955608E-4	1.8345495499882107
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26059	0.7973770612192442	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20261	0.6199645664593081	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13648	0.4176139579999327	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5493	0.16807982644296823	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4128	0.126312310860472	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3985	0.12193666637087718	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3940	0.12055971530771797	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3878	0.1186625827318097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.059891251464923E-5	0.0
5	0.0	0.0	0.0	3.059891251464923E-5	0.0
6	0.0	0.0	0.0	3.059891251464923E-5	0.0
7	0.0	0.0	0.0	3.059891251464923E-5	0.0
8	0.0	0.0	0.0	3.059891251464923E-5	0.0
9	0.0	0.0	0.0	3.059891251464923E-5	0.0
10-11	0.0	0.0	0.0	3.059891251464923E-5	0.0
12-13	0.0	0.0	0.0	3.059891251464923E-5	0.0
14-15	0.0	0.0	0.0	7.649728128662309E-5	1.5299456257324616E-5
16-17	0.0	0.0	0.0	1.5299456257324615E-4	6.119782502929846E-5
18-19	0.0	0.0	0.0	2.141923876025446E-4	6.119782502929846E-5
20-21	0.0	0.0	0.0	2.4479130011719385E-4	6.119782502929846E-5
22-23	0.0	0.0	0.0	3.3658803766114154E-4	9.17967375439477E-5
24-25	0.0	0.0	0.0	6.578766190649584E-4	1.2239565005859693E-4
26-27	0.0	0.0	0.0	8.873684629248277E-4	1.3769510631592152E-4
28-29	0.0	0.0	0.0	0.0021266244197681214	1.5299456257324615E-4
30-31	0.0	0.0	0.0	0.004880526546086552	1.5299456257324615E-4
32-33	0.0	0.0	0.0	0.010296534061179466	1.5299456257324615E-4
34-35	0.0	0.0	0.0	0.017854465452297824	1.5299456257324615E-4
36-37	0.0	0.0	0.0	0.029971634808098918	1.5299456257324615E-4
38-39	0.0	0.0	0.0	0.04900415839221074	1.5299456257324615E-4
40-41	0.0	0.0	0.0	0.06893934989550471	1.5299456257324615E-4
42-43	0.0	0.0	0.0	0.08970071203669422	1.5299456257324615E-4
44-45	0.0	0.0	0.0	0.1154802958302862	1.5299456257324615E-4
46-47	0.0	0.0	0.0	0.1486648164524233	1.5299456257324615E-4
48-49	0.0	0.0	0.0	0.18761723208357176	1.5299456257324615E-4
50-51	0.0	0.0	0.0	0.2276100107402183	1.5299456257324615E-4
52-53	0.0	0.0	0.0	0.2663329345275069	1.5299456257324615E-4
54-55	0.0	0.0	0.0	0.3103953685486018	1.5299456257324615E-4
56-57	0.0	0.0	0.0	0.36408116055555384	1.9889293134522E-4
58-59	0.0	0.0	0.0	0.42504949374099243	2.7539021263184305E-4
60-61	0.0	0.0	0.0	0.4958094789311188	2.7539021263184305E-4
62-63	0.0	0.0	0.0	0.5668295548776197	2.7539021263184305E-4
64-65	0.0	0.0	0.0	0.6414297035883345	2.7539021263184305E-4
66-67	0.0	0.0	0.0	0.7275809417733294	2.7539021263184305E-4
68-69	0.0	0.0	0.0	0.8297813095722578	3.059891251464923E-4
70-71	0.0	0.0	0.0	0.9473882298223122	3.212885814038169E-4
72-73	0.0	0.0	0.0	1.0733639526451229	3.3658803766114154E-4
74-75	0.0	0.0	0.0	1.1983452108112078	3.3658803766114154E-4
76-77	0.0	0.0	0.0	1.3390390105535648	3.3658803766114154E-4
78-79	0.0	0.0	0.0	1.502070016431616	3.6718695017579073E-4
80-81	0.0	0.0	0.0	1.6874229289891036	3.6718695017579073E-4
82-83	0.0	0.0	0.0	1.8864076570718677	3.6718695017579073E-4
84-85	0.0	0.0	0.0	2.085116994942	3.6718695017579073E-4
86-87	0.0	0.0	0.0	2.2960199994492196	3.6718695017579073E-4
88	0.0	0.0	0.0	2.472086142058511	3.6718695017579073E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8995	0.0	65.29072	1
GTATCAA	17955	0.0	45.43644	1
ATCAACG	23045	0.0	35.214996	3
TATCAAC	23295	0.0	34.837605	2
TCAACGC	23350	0.0	34.83449	4
CAACGCA	24165	0.0	33.640194	5
AACGCAG	24960	0.0	32.556263	6
GTGGTAT	2905	0.0	31.239305	1
ATAACGC	360	0.0	30.03924	3
TATAACG	315	0.0	29.853117	2
TGGTATC	2955	0.0	29.27727	2
ACGCAGA	27895	0.0	28.928642	7
CGCAGAG	27985	0.0	28.751638	8
GTACATG	16335	0.0	27.144558	1
TACATGG	16520	0.0	26.61165	2
GCAGAGT	30135	0.0	26.481993	9
CATGGGT	7835	0.0	26.223627	4
ACATGGG	16515	0.0	26.049902	3
GAGTACT	21335	0.0	22.997751	12-13
CAGAGTA	29915	0.0	22.57591	10-11
>>END_MODULE