FastQCFastQC Report
Wed 25 May 2016
SRR2431407_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431407_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3507256
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218320.6224809366638763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43200.049.32981
GTATCAA76900.037.377461
TCAACGC86150.032.160584
ATCAACG86450.032.104313
CAACGCA94450.029.4335885
TATCAAC95150.029.416062
GTACATG181050.028.4518491
GTCGTAC2203.6379788E-1227.7981281
TACATGG178550.027.7161052
AACGCAG101000.027.498076
CATGGGT76200.027.2854564
GCGCGTA700.00884211626.8548569
ACATGGG185350.025.7602313
CGCAGAG113550.023.7563348
ACGCAGA116200.023.4568867
ATGGGTG41400.023.1786165
GCAGAGT127000.020.833669
CATGGGG81400.020.5148264
ATGGGTA35800.019.5776675
GTACCGT1708.64328E-519.350196
Produced by FastQC (version 0.11.5)