##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431407_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3507256
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.274654601774152	31.0	31.0	34.0	26.0	34.0
2	30.25876155034021	31.0	31.0	34.0	26.0	34.0
3	30.44415491769064	33.0	31.0	34.0	26.0	34.0
4	33.70278188988771	37.0	35.0	37.0	28.0	37.0
5	33.78806736662508	37.0	35.0	37.0	28.0	37.0
6	33.81261504720499	37.0	35.0	37.0	29.0	37.0
7	33.62761999694348	37.0	35.0	37.0	28.0	37.0
8	33.63547029358564	37.0	35.0	37.0	28.0	37.0
9	35.0036986179509	39.0	37.0	39.0	27.0	39.0
10-11	35.048825349504	39.0	37.0	39.0	27.0	39.0
12-13	34.98879309066689	39.0	37.0	39.0	27.0	39.0
14-15	36.23810808221584	40.0	37.0	41.0	27.0	41.0
16-17	36.233057125000286	40.0	37.0	41.0	27.0	41.0
18-19	36.22436913644171	40.0	37.0	41.0	27.0	41.0
20-21	36.306737375315635	40.0	37.0	41.0	27.0	41.0
22-23	36.254166932781644	40.0	37.0	41.0	27.0	41.0
24-25	36.29388202629064	40.0	37.0	41.0	27.0	41.0
26-27	36.334531896160414	40.0	37.0	41.0	27.5	41.0
28-29	36.2969194435764	40.0	37.0	41.0	28.0	41.0
30-31	36.22666665906338	40.0	37.0	41.0	28.0	41.0
32-33	36.181947796225884	40.0	36.5	41.0	28.0	41.0
34-35	36.10593024860461	40.0	36.0	41.0	27.5	41.0
36-37	35.95253782444167	40.0	36.0	41.0	27.0	41.0
38-39	35.81440747410511	40.0	36.0	41.0	27.0	41.0
40-41	35.74660518080232	40.0	36.0	41.0	27.0	41.0
42-43	35.54979419808534	40.0	35.0	41.0	26.0	41.0
44-45	35.357704142497724	40.0	35.0	41.0	25.5	41.0
46-47	35.20276449737345	40.0	35.0	41.0	25.0	41.0
48-49	35.05052297294523	39.0	35.0	41.0	25.0	41.0
50-51	34.89177279902009	39.0	34.5	41.0	24.0	41.0
52-53	34.714728266200126	39.0	34.0	41.0	24.0	41.0
54-55	34.53400721247608	39.0	34.0	41.0	23.5	41.0
56-57	34.32454745248137	38.5	34.0	40.0	23.0	41.0
58-59	34.09696939715835	38.0	33.0	40.0	22.5	41.0
60-61	33.846910804343906	38.0	33.0	40.0	22.0	41.0
62-63	33.74689144447967	38.0	33.0	40.0	21.5	41.0
64-65	33.4983442896669	37.0	33.0	40.0	20.0	41.0
66-67	33.23890656969437	37.0	33.0	40.0	19.5	41.0
68-69	32.94704421348199	36.5	32.0	40.0	18.0	41.0
70-71	32.61907371460766	36.0	32.0	39.0	16.0	41.0
72-73	32.286619083408794	35.5	32.0	39.0	13.0	41.0
74-75	32.00400113364978	35.0	32.0	39.0	10.5	40.0
76-77	31.752885874313137	35.0	32.0	37.5	7.5	40.0
78-79	31.40948778760376	35.0	31.0	37.0	6.5	39.0
80-81	31.08390505283903	35.0	31.0	37.0	2.0	39.0
82-83	30.756291243068656	35.0	31.0	36.0	2.0	39.0
84-85	30.454943978996685	35.0	31.0	36.0	2.0	37.0
86-87	30.17326650806214	35.0	31.0	35.5	2.0	37.0
88-89	29.902496424555267	35.0	30.5	35.0	2.0	37.0
90-91	29.663197953043632	34.5	30.0	35.0	2.0	36.0
92-93	29.424487690661874	34.0	30.0	35.0	2.0	36.0
94-95	29.195182359086417	34.0	30.0	35.0	2.0	36.0
96-97	28.97828829717591	34.0	30.0	35.0	2.0	35.5
98-99	28.73922804608503	34.0	29.5	35.0	2.0	35.0
100	28.549026076225974	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4420582755718243
1101	2	0.4277746815396206
1101	3	0.4256100575399735
1101	4	0.5669673552877015
1101	5	0.47131698311631
1101	6	0.5113138369459094
1101	7	0.4486614885964073
1101	8	0.4934114147305806
1101	9	0.6108890924597148
1101	10-11	0.5152090017054789
1101	12-13	0.49974339338783835
1101	14-15	0.6248788998019421
1101	16-17	0.5229839349257261
1101	18-19	0.4261945667746616
1101	20-21	0.5290189260139115
1101	22-23	0.5788410900686216
1101	24-25	0.5110202823652585
1101	26-27	0.5084496220868076
1101	28-29	0.45502138575261597
1101	30-31	0.4612212937735265
1101	32-33	0.43365321224059983
1101	34-35	0.4408601489666282
1101	36-37	0.4000023357004423
1101	38-39	0.4760146595084862
1101	40-41	0.4083444139618422
1101	42-43	0.4282593311121303
1101	44-45	0.39968404503884614
1101	46-47	0.5073963560391235
1101	48-49	0.5031909205925231
1101	50-51	0.5199567562955778
1101	52-53	0.4666717467404524
1101	54-55	0.5061470231678129
1101	56-57	0.4498835728089645
1101	58-59	0.54755557312577
1101	60-61	0.42853305942001185
1101	62-63	0.31676066881726683
1101	64-65	0.45815679330055303
1101	66-67	0.5002237220525174
1101	68-69	0.4854413369827171
1101	70-71	0.4337041363999674
1101	72-73	0.44751936736956566
1101	74-75	0.45765327621417384
1101	76-77	0.5045966496414636
1101	78-79	0.38288441617298474
1101	80-81	0.37468453004176183
1101	82-83	0.4776168342318243
1101	84-85	0.40720919710937764
1101	86-87	0.46383329735280654
1101	88-89	0.3420721921072598
1101	90-91	0.25700055915913467
1101	92-93	0.24048089841272002
1101	94-95	0.2676502702910284
1101	96-97	0.26419544980491594
1101	98-99	0.3309202910560316
1101	100	0.2521847066334679
1102	1	0.17676395992577199
1102	2	0.14739753193900285
1102	3	0.22055700665272227
1102	4	0.2769516355514554
1102	5	0.25536682447684456
1102	6	0.2230061641967893
1102	7	0.15153817132601688
1102	8	0.08990861729104438
1102	9	0.22557142440106048
1102	10-11	0.22764650987270585
1102	12-13	0.23247021334287155
1102	14-15	0.2508878688814633
1102	16-17	0.2147789770214601
1102	18-19	0.24844247294479516
1102	20-21	0.23092492792071084
1102	22-23	0.1740217486056963
1102	24-25	0.26889787466755166
1102	26-27	0.20512167368158174
1102	28-29	0.22759892944231552
1102	30-31	0.20240035213941354
1102	32-33	0.3026696701083722
1102	34-35	0.2604324290583051
1102	36-37	0.26438009372382254
1102	38-39	0.28720561804985323
1102	40-41	0.23886457755280333
1102	42-43	0.22645457858277496
1102	44-45	0.24606928314950238
1102	46-47	0.20589081586332725
1102	48-49	0.14917487924297035
1102	50-51	0.10761470894978231
1102	52-53	0.167893937703667
1102	54-55	0.13274930916808358
1102	56-57	0.17094220262351456
1102	58-59	0.13969905245640035
1102	60-61	0.1465002297273088
1102	62-63	0.194191862342592
1102	64-65	0.13579345840910406
1102	66-67	0.1948138270296127
1102	68-69	0.1405468433858985
1102	70-71	0.1652231340621455
1102	72-73	0.1171121350018609
1102	74-75	0.12022485168913022
1102	76-77	0.07646997138318312
1102	78-79	0.03878974633336796
1102	80-81	0.02079906353087324
1102	82-83	-0.02382410130839574
1102	84-85	0.014253817409912273
1102	86-87	-0.06250936566654275
1102	88-89	-0.03751704116244525
1102	90-91	0.025280533043360975
1102	92-93	0.007372223087308782
1102	94-95	-0.030046343633308936
1102	96-97	-9.908289084563648E-4
1102	98-99	-0.0660641975473979
1102	100	-0.16382177461571956
1103	1	-0.2687740574215418
1103	2	-0.27245655494074583
1103	3	-0.22308354359364557
1103	4	-0.1833243227863548
1103	5	-0.12675004918840216
1103	6	-0.14577403552403467
1103	7	-0.3514705261935802
1103	8	-0.20034395676911032
1103	9	-0.23555448084489683
1103	10-11	-0.22432409274967569
1103	12-13	-0.19595874498299537
1103	14-15	-0.20636197303604575
1103	16-17	-0.25572247256513947
1103	18-19	-0.22905326181068375
1103	20-21	-0.24974951759750041
1103	22-23	-0.2483523521137272
1103	24-25	-0.12469256641242055
1103	26-27	-0.1599275406782752
1103	28-29	-0.18008173199867628
1103	30-31	-0.23213267815277305
1103	32-33	-0.19417631578947692
1103	34-35	-0.28685272789076777
1103	36-37	-0.35557946102044724
1103	38-39	-0.2752880288236881
1103	40-41	-0.2886771502589838
1103	42-43	-0.4072129948211938
1103	44-45	-0.4669620231487386
1103	46-47	-0.5728996443058563
1103	48-49	-0.5132698515395617
1103	50-51	-0.5496117486900758
1103	52-53	-0.5728880635609954
1103	54-55	-0.552699943370186
1103	56-57	-0.5540162186991324
1103	58-59	-0.6138467079502021
1103	60-61	-0.630581627175232
1103	62-63	-0.5628848046820067
1103	64-65	-0.525660363607912
1103	66-67	-0.6006294601316995
1103	68-69	-0.5021325653259439
1103	70-71	-0.4601644364784363
1103	72-73	-0.5177375971527098
1103	74-75	-0.5429945429444203
1103	76-77	-0.5917987569177612
1103	78-79	-0.6222151214142251
1103	80-81	-0.6224851747501461
1103	82-83	-0.6061039490719757
1103	84-85	-0.5627587218124397
1103	86-87	-0.6696800470319033
1103	88-89	-0.6820709502739639
1103	90-91	-0.6389347812723507
1103	92-93	-0.7104896068517235
1103	94-95	-0.7688723574491263
1103	96-97	-0.7750316559483181
1103	98-99	-0.8306169732372979
1103	100	-0.7843608060771174
1104	1	-0.16397817824347882
1104	2	-0.13296380068022984
1104	3	-0.005178021117636433
1104	4	-0.08987817696859679
1104	5	-0.016821671043764752
1104	6	0.015455174935162574
1104	7	-0.05546417023032291
1104	8	-0.1075562746728167
1104	9	-0.029264070785586682
1104	10-11	-0.0998899288608115
1104	12-13	-0.12003729155479448
1104	14-15	-0.07502994724866596
1104	16-17	-0.02318504186361281
1104	18-19	-0.113009555768123
1104	20-21	-0.20499233708873277
1104	22-23	-0.0794778677295227
1104	24-25	-0.019340950889564112
1104	26-27	-0.0868023592782663
1104	28-29	-0.050448167352847406
1104	30-31	-0.047840292115957084
1104	32-33	-0.11927609679019469
1104	34-35	-0.03451746841911785
1104	36-37	-0.14062330485372598
1104	38-39	-0.07607935797639698
1104	40-41	-0.056082199262633026
1104	42-43	-0.16759394217634593
1104	44-45	-0.20615697355874119
1104	46-47	-0.28516454256060797
1104	48-49	-0.2648402845835349
1104	50-51	-0.35786570577559473
1104	52-53	-0.41565566339329507
1104	54-55	-0.28799576831148244
1104	56-57	-0.3612032065647597
1104	58-59	-0.3749333783108355
1104	60-61	-0.40866023824480635
1104	62-63	-0.3453035301471914
1104	64-65	-0.3142404449337519
1104	66-67	-0.2672192571689038
1104	68-69	-0.2524283462914738
1104	70-71	-0.43144896844442115
1104	72-73	-0.31447518206257286
1104	74-75	-0.34424034692393235
1104	76-77	-0.2792967999964162
1104	78-79	-0.2708865550533375
1104	80-81	-0.3373566629191984
1104	82-83	-0.3851544830842961
1104	84-85	-0.37307262079132286
1104	86-87	-0.29045918024034023
1104	88-89	-0.3496678508623603
1104	90-91	-0.37077628090441905
1104	92-93	-0.37837616111634986
1104	94-95	-0.41011259116563537
1104	96-97	-0.439737062328323
1104	98-99	-0.42592763266848266
1104	100	-0.38244428379752904
1105	1	-0.1400582034615283
1105	2	-0.07829830890385026
1105	3	-0.012117102507154698
1105	4	0.048651666830778595
1105	5	-0.06198860794739858
1105	6	-0.026391522622702723
1105	7	-0.08276351775749902
1105	8	-0.09203495828327846
1105	9	-0.16288923564233926
1105	10-11	-0.1123882170041881
1105	12-13	-0.17372540469685305
1105	14-15	-0.25188227525167406
1105	16-17	-0.27383781587962375
1105	18-19	-0.15303432698173225
1105	20-21	-0.12666237180472706
1105	22-23	-0.1852480560474774
1105	24-25	-0.2176372760938321
1105	26-27	-0.15972138975587313
1105	28-29	-0.13997083462221127
1105	30-31	-0.17214411166842325
1105	32-33	-0.14406261797421394
1105	34-35	-0.1823357166735562
1105	36-37	-0.17851698612782485
1105	38-39	-0.2156737554699646
1105	40-41	-0.3397991219224039
1105	42-43	-0.3329497612993606
1105	44-45	-0.34040090405476775
1105	46-47	-0.31834817803472504
1105	48-49	-0.4694581646324849
1105	50-51	-0.3583099116022197
1105	52-53	-0.3803173379962672
1105	54-55	-0.4468971444764591
1105	56-57	-0.5390555600359264
1105	58-59	-0.38460479638999345
1105	60-61	-0.37637370714803353
1105	62-63	-0.27894771605680546
1105	64-65	-0.38318398386068253
1105	66-67	-0.3595633234239415
1105	68-69	-0.2926522495137007
1105	70-71	-0.2762164937387226
1105	72-73	-0.2321211336627087
1105	74-75	-0.29376383313940835
1105	76-77	-0.30885383478927153
1105	78-79	-0.3335512783624033
1105	80-81	-0.3420002932569872
1105	82-83	-0.27119010602015337
1105	84-85	-0.24494487953315414
1105	86-87	-0.3176237524180152
1105	88-89	-0.31285849160457957
1105	90-91	-0.334283291052639
1105	92-93	-0.40853045849413405
1105	94-95	-0.49469628517076814
1105	96-97	-0.3914925579599995
1105	98-99	-0.48722656889255944
1105	100	-0.510842605156256
1106	1	-0.23963014168353425
1106	2	-0.29310112479779704
1106	3	-0.2197913672393348
1106	4	-0.1875183239895506
1106	5	-0.104356068993404
1106	6	-0.09541832845477671
1106	7	-0.09606673898757379
1106	8	-0.2629197534097045
1106	9	-0.13615493347617047
1106	10-11	-0.16186422319152172
1106	12-13	-0.12887917164049867
1106	14-15	-0.19369374030335962
1106	16-17	-0.14517052978384726
1106	18-19	-0.21022867075445362
1106	20-21	-0.2623814634345365
1106	22-23	-0.1734835101431642
1106	24-25	-0.2310211919985008
1106	26-27	-0.28642566426380967
1106	28-29	-0.2594921654361002
1106	30-31	-0.16400460450947918
1106	32-33	-0.2514535572214811
1106	34-35	-0.11519804544884948
1106	36-37	-0.21326941814461264
1106	38-39	-0.25095632840066173
1106	40-41	-0.23752532779739255
1106	42-43	-0.212435729516244
1106	44-45	-0.2811572657457333
1106	46-47	-0.3400825398091456
1106	48-49	-0.2943052694595636
1106	50-51	-0.2997974803007395
1106	52-53	-0.41536617205881043
1106	54-55	-0.3625828703857863
1106	56-57	-0.4468460236093179
1106	58-59	-0.47934302674013196
1106	60-61	-0.4710418977990827
1106	62-63	-0.4278482790567679
1106	64-65	-0.3159378628678482
1106	66-67	-0.2913265504956186
1106	68-69	-0.2796577990421909
1106	70-71	-0.3120453390672253
1106	72-73	-0.30929038981129864
1106	74-75	-0.2994996374335841
1106	76-77	-0.2736568369590131
1106	78-79	-0.36105203091661764
1106	80-81	-0.3833383942572155
1106	82-83	-0.39486408314250454
1106	84-85	-0.4103100247984699
1106	86-87	-0.4089286218163295
1106	88-89	-0.45533514752188964
1106	90-91	-0.4356643770554314
1106	92-93	-0.4148531060543661
1106	94-95	-0.44824621751539695
1106	96-97	-0.45869453158930895
1106	98-99	-0.5219256887766797
1106	100	-0.5779336329217806
1107	1	-0.25199360588585407
1107	2	-0.2765496237180969
1107	3	-0.21877174384287557
1107	4	-0.20087799552066343
1107	5	-0.16802355205151542
1107	6	-0.2588681152587924
1107	7	-0.31901287544616963
1107	8	-0.26061370979581966
1107	9	-0.23753113967806172
1107	10-11	-0.23497218663025876
1107	12-13	-0.21262205443649407
1107	14-15	-0.3029778452571037
1107	16-17	-0.30525250065572607
1107	18-19	-0.3582164622021722
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2216	88-89	0.2163719815106262
2216	90-91	0.25367122634645867
2216	92-93	0.265620636908551
2216	94-95	0.24200547098194392
2216	96-97	0.28496209912568204
2216	98-99	0.21296553264334506
2216	100	0.21382650006997395
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106556.0
3	20818.0
4	14770.0
5	13347.0
6	9444.0
7	7817.0
8	8417.0
9	9000.0
10	9453.0
11	10326.0
12	11471.0
13	12375.0
14	12981.0
15	13063.0
16	13208.0
17	12682.0
18	12634.0
19	12507.0
20	12305.0
21	12884.0
22	14944.0
23	21505.0
24	33579.0
25	42508.0
26	46310.0
27	38366.0
28	37491.0
29	44809.0
30	53486.0
31	62464.0
32	79524.0
33	105244.0
34	148675.0
35	229681.0
36	370746.0
37	624172.0
38	848928.0
39	367796.0
40	970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38256989711969	21.51032787404966	13.862951349131297	22.244150879699355
2	14.989711184891648	22.544857377458854	39.87559173181964	22.589839705829853
3	17.25210751564659	28.965047217548058	30.428489858245953	23.3543554085594
4	11.677835404140929	19.163959807967977	39.08007075104583	30.078134036845267
5	11.909974928924342	40.99119441666546	34.460260616930704	12.638570037479502
6	27.909444509753122	40.334816664593276	18.772605203719323	12.98313362193428
7	25.241307563424197	35.37211640743057	22.963765915970068	16.42281011317516
8	24.055166353545683	37.74173439066942	20.759906819760715	17.443192436024177
9	25.190273255160445	18.656963809995485	21.069050690655256	35.08371224418882
10-11	22.392310136193814	29.578111181981736	28.958322128530657	19.071256553293797
12-13	23.139682470390575	27.831667564710898	28.4203822800965	20.608267684802026
14-15	21.104385533066218	29.186439328782527	25.956074340121503	23.753100798029756
16-17	20.35168599896407	32.05939358786736	26.607045217647578	20.98187519552099
18-19	20.438867439601836	30.995706592013256	28.399518617892394	20.16590735049251
20-21	22.22519270025155	29.896682050540957	27.6630125507135	20.215112698493993
22-23	21.791649940126835	29.913672509043742	27.699577934833684	20.595099615995736
24-25	21.331156978707362	30.034506975647396	27.74352853334155	20.890807512303695
26-27	21.024352546779394	30.024450554427982	27.848224242641255	21.102972656151366
28-29	21.027646940753215	29.96719868994481	28.060191894725385	20.944962474576595
30-31	21.288039296781523	30.298131274316354	27.688460786178158	20.725368642723964
32-33	20.925400985218864	30.09427057996094	28.043143627106215	20.937184807713983
34-35	21.24883447023605	29.819258717363738	27.986209942582324	20.945696869817887
36-37	21.389540558382667	29.93163997710917	27.606303452414434	21.072516012093732
38-39	21.194608995526803	30.000017120097194	27.84399767965616	20.96137620471984
40-41	21.09536721145613	29.741047866356197	28.049229352264437	21.11435556992324
42-43	21.03137657164624	29.82628966654635	28.04249169326202	21.099842068545392
44-45	21.19266650638888	29.81241078008437	27.939101098426022	21.055821615100736
46-47	21.211782700480633	29.911048090014212	27.8410247652652	21.036144444239955
48-49	21.160846833363415	29.752951054618144	27.859967313151003	21.226234798867445
50-51	21.07140258491425	29.717411433373332	27.890636926408842	21.320549055303584
52-53	21.15532991042105	29.78679844707754	27.81060589199736	21.24726575050405
54-55	21.28046971871406	29.91733212005882	27.76703702641597	21.035161134811148
56-57	21.19110271622093	29.72712247781778	27.673340176373536	21.408434629587756
58-59	21.184536120128016	29.484526874945782	27.88288465664991	21.448052348276285
60-61	21.306343567108378	29.844660088905073	27.62986580350547	21.219130540481082
62-63	21.11996142540545	29.749728772382074	27.803666155714968	21.32664364649751
64-65	21.222625316170376	29.592134865122038	27.77543022710056	21.409809591607022
66-67	21.010687015059858	29.584451557201035	27.767493510354612	21.63736791738449
68-69	21.435049163840237	29.757130272585584	27.573849188092257	21.233971375481918
70-71	21.30053219982251	29.580321488236528	27.70514700438419	21.413999307556775
72-73	21.214165857961685	29.641810858182083	27.862740737433384	21.28128254642285
74-75	21.15154992523348	29.72924276821865	27.751432204697924	21.367775101849947
76-77	21.191283634266217	29.619881985556418	27.668368028959634	21.520466351217728
78-79	21.303860675469128	29.58433634734467	27.824633443970402	21.287169533215803
80-81	21.127047914416927	29.59273337696206	27.869840863569202	21.410377845051812
82-83	21.127175320390496	29.564197588109614	27.98905696584047	21.319570125659418
84-85	21.098057366312226	29.484695644258498	27.819438378732603	21.597808610696678
86-87	21.32103709558451	29.572389962320894	27.710063502344777	21.396509439749813
88-89	21.20209346816304	29.4296978515885	27.853989683892472	21.51421899635599
90-91	21.15941330109372	29.425526484327776	27.75222162126456	21.662838593313943
92-93	21.195097824669684	29.518846937695386	27.737337487906196	21.548717749728734
94-95	21.09769703941751	29.33982595516593	27.939572624223537	21.622904381193024
96-97	21.114839345831044	29.468973109490886	27.74798566193301	21.668201882745063
98-99	21.009687872012346	29.501813051919367	27.73457250895112	21.753926567117162
100	21.010223917140273	29.51586778878257	27.727367155851674	21.746541138225485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19453.0
1	14334.0
2	7823.0
3	5329.0
4	3525.5
5	2438.0
6	2048.0
7	2099.5
8	2370.0
9	2473.5
10	1979.5
11	1482.0
12	1393.5
13	1433.5
14	1499.0
15	1609.5
16	1841.0
17	2233.5
18	2786.5
19	3633.0
20	4886.5
21	6680.0
22	9672.5
23	14394.0
24	19949.0
25	25474.5
26	32489.5
27	41639.0
28	53149.0
29	65046.5
30	72947.5
31	79698.5
32	87951.0
33	96097.0
34	103479.5
35	109039.0
36	112779.0
37	115561.0
38	117107.5
39	117739.5
40	118528.0
41	118127.0
42	116251.5
43	114683.5
44	113304.5
45	110533.5
46	107974.0
47	105959.5
48	103643.0
49	101766.0
50	100890.5
51	96825.5
52	90004.0
53	84483.5
54	80867.5
55	78701.5
56	72364.0
57	63856.5
58	57816.5
59	53241.0
60	47661.5
61	40293.0
62	33788.5
63	28040.5
64	22554.0
65	18485.0
66	15140.5
67	11959.0
68	9305.0
69	7214.5
70	5465.0
71	4222.5
72	3386.5
73	2631.0
74	2168.0
75	1776.5
76	1262.5
77	1001.0
78	761.0
79	543.5
80	392.5
81	267.5
82	210.5
83	166.0
84	135.5
85	119.5
86	96.0
87	78.5
88	60.0
89	52.0
90	51.0
91	41.0
92	35.5
93	38.5
94	35.5
95	26.5
96	22.0
97	18.0
98	21.0
99	18.5
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17115944772779632
2	0.16768094487542398
3	0.18056851282027886
4	0.17007597962623772
5	0.18273544902339606
6	0.07219319034595707
7	0.07413202800137772
8	0.0633258593042538
9	0.057994055751847025
10-11	0.0694132392959054
12-13	0.06892852988205024
14-15	0.065906224125071
16-17	0.0626558198203952
18-19	0.06694692374893649
20-21	0.06830125887588473
22-23	0.06691841143047442
24-25	0.06593473644353307
26-27	0.06921365306667092
28-29	0.0674458893220227
30-31	0.07246405737134672
32-33	0.06988369255052954
34-35	0.06877171213050887
36-37	0.07970618626071208
38-39	0.07421756495676392
40-41	0.0706250128305433
42-43	0.07330517076597773
44-45	0.08335576302385682
46-47	0.07440289502676736
48-49	0.0795066000314776
50-51	0.07672664898142594
52-53	0.0752154961029363
54-55	0.0767123928221949
56-57	0.07066778130823641
58-59	0.07778160476452246
60-61	0.07467376205215702
62-63	0.06724630309278821
64-65	0.06902832299666747
66-67	0.06962708168437091
68-69	0.0682442342389606
70-71	0.06487978066043654
72-73	0.06177193794807108
74-75	0.06686138679355029
76-77	0.07172273709133294
78-79	0.06851510126435024
80-81	0.07654131891142249
82-83	0.07822354570068453
84-85	0.08019089567456725
86-87	0.07587127942756389
88-89	0.07802395947145005
90-91	0.07597107254218112
92-93	0.06737460852586752
94-95	0.06713225381893993
96-97	0.06616283499122962
98-99	0.07607086565679835
100	0.08337001918308785
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3507256.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.91186107582634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.90827969503712	47.61534728059934
2	14.044720948234243	17.39069624391653
3	4.391400366303866	8.156393082208135
4	1.7463046719533026	4.324678889841574
5	0.8646054967772614	2.6764667700934814
6	0.48167836891174537	1.7892962555576768
7	0.3119528328765943	1.3519506315886325
8	0.21305693087310895	1.0552600884366286
9	0.1622320865805127	0.9039681365772718
>10	0.7975609997747327	8.878745896970052
>50	0.053681236270546516	2.294505182140179
>100	0.023375907703353047	2.351632449923333
>500	8.283302720900645E-4	0.3115755624908937
>1k	2.761100842315814E-4	0.27747205015119364
>5k	0.0	0.0
>10k+	4.6018347371930176E-5	0.6220114795051706
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21832	0.6224809366638763	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	2.8512318462068352E-5	0.0	2.8512318462068352E-5	0.0
14-15	0.0	2.8512318462068352E-5	0.0	2.8512318462068352E-5	0.0
16-17	0.0	2.8512318462068352E-5	0.0	2.8512318462068352E-5	0.0
18-19	0.0	2.8512318462068352E-5	0.0	4.276847769310253E-5	0.0
20-21	0.0	2.8512318462068352E-5	0.0	1.1404927384827341E-4	0.0
22-23	0.0	2.8512318462068352E-5	0.0	2.1384238846551264E-4	0.0
24-25	0.0	2.8512318462068352E-5	0.0	4.419409361620595E-4	0.0
26-27	0.0	2.8512318462068352E-5	0.0	8.981380315551531E-4	0.0
28-29	0.0	2.8512318462068352E-5	0.0	0.0021669362031171945	0.0
30-31	0.0	2.8512318462068352E-5	0.0	0.006215685424730901	2.8512318462068352E-5
32-33	0.0	5.7024636924136704E-5	0.0	0.013757193657947982	2.8512318462068352E-5
34-35	0.0	5.7024636924136704E-5	0.0	0.023879066711982245	2.8512318462068352E-5
36-37	0.0	5.7024636924136704E-5	0.0	0.04082964003768188	2.8512318462068352E-5
38-39	0.0	5.7024636924136704E-5	0.0	0.06443783972427447	2.8512318462068352E-5
40-41	0.0	5.7024636924136704E-5	0.0	0.0920662763140187	2.8512318462068352E-5
42-43	0.0	5.7024636924136704E-5	0.0	0.11819781618450435	2.8512318462068352E-5
44-45	0.0	5.7024636924136704E-5	0.0	0.14894835164584508	2.8512318462068352E-5
46-47	0.0	5.7024636924136704E-5	0.0	0.18926476995120972	2.8512318462068352E-5
48-49	0.0	5.7024636924136704E-5	0.0	0.23478468637590183	2.8512318462068352E-5
50-51	0.0	5.7024636924136704E-5	0.0	0.2813167901059974	2.8512318462068352E-5
52-53	0.0	5.7024636924136704E-5	0.0	0.32660860798299296	2.8512318462068352E-5
54-55	0.0	5.7024636924136704E-5	0.0	0.37677603231700224	2.8512318462068352E-5
56-57	0.0	5.7024636924136704E-5	0.0	0.44030147785049056	2.8512318462068352E-5
58-59	0.0	5.7024636924136704E-5	0.0	0.5132645007949235	2.8512318462068352E-5
60-61	0.0	5.7024636924136704E-5	0.0	0.5914167656994528	2.8512318462068352E-5
62-63	0.0	5.7024636924136704E-5	0.0	0.6715363805778649	2.8512318462068352E-5
64-65	0.0	5.7024636924136704E-5	0.0	0.7580284986325492	2.8512318462068352E-5
66-67	0.0	5.7024636924136704E-5	0.0	0.8596749139498229	2.8512318462068352E-5
68-69	0.0	5.7024636924136704E-5	0.0	0.9800539224966754	2.8512318462068352E-5
70-71	0.0	5.7024636924136704E-5	0.0	1.1134630605806932	2.8512318462068352E-5
72-73	0.0	5.7024636924136704E-5	0.0	1.2547986231971662	2.8512318462068352E-5
74-75	0.0	5.7024636924136704E-5	0.0	1.3955639394443975	2.8512318462068352E-5
76-77	0.0	5.7024636924136704E-5	0.0	1.5512126859288287	2.8512318462068352E-5
78-79	0.0	5.7024636924136704E-5	0.0	1.7342190019776145	2.8512318462068352E-5
80-81	0.0	5.7024636924136704E-5	0.0	1.9442692520876719	2.8512318462068352E-5
82-83	0.0	7.128079615517088E-5	0.0	2.1650971585763914	2.8512318462068352E-5
84-85	0.0	8.553695538620506E-5	0.0	2.3818763158434972	2.8512318462068352E-5
86-87	0.0	8.553695538620506E-5	0.0	2.615320923251682	2.8512318462068352E-5
88	0.0	8.553695538620506E-5	0.0	2.809062127201436	2.8512318462068352E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4320	0.0	49.3298	1
GTATCAA	7690	0.0	37.37746	1
TCAACGC	8615	0.0	32.16058	4
ATCAACG	8645	0.0	32.10431	3
CAACGCA	9445	0.0	29.433588	5
TATCAAC	9515	0.0	29.41606	2
GTACATG	18105	0.0	28.451849	1
GTCGTAC	220	3.6379788E-12	27.798128	1
TACATGG	17855	0.0	27.716105	2
AACGCAG	10100	0.0	27.49807	6
CATGGGT	7620	0.0	27.285456	4
GCGCGTA	70	0.008842116	26.854856	9
ACATGGG	18535	0.0	25.760231	3
CGCAGAG	11355	0.0	23.756334	8
ACGCAGA	11620	0.0	23.456886	7
ATGGGTG	4140	0.0	23.178616	5
GCAGAGT	12700	0.0	20.83366	9
CATGGGG	8140	0.0	20.514826	4
ATGGGTA	3580	0.0	19.577667	5
GTACCGT	170	8.64328E-5	19.35019	6
>>END_MODULE