FastQCFastQC Report
Wed 25 May 2016
SRR2431407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3507256
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258250.7363306242829152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208500.5944818399341251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137000.3906187629303364No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52120.14860620382430026No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42280.120550082457625No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41700.11889636798682504No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38100.10863193334048044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37350.10649350945582528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95050.065.2081761
GTATCAA191400.045.47821
ATCAACG243800.035.3695873
TCAACGC246600.035.0061234
TATCAAC248500.034.910252
CAACGCA254600.033.8322945
AACGCAG264400.032.7243466
GTGGTAT29750.032.7206651
TGGTATC31550.030.4028152
ACGCAGA296950.028.9790157
CGCAGAG297700.028.8112878
CATGGGT74450.028.0403024
GCAGAGT319500.026.580679
GTACATG178700.026.4735491
TACATGG180200.025.7801322
ACATGGG177700.025.2950123
TATAACG3400.024.8929622
GAGTACT224900.023.13325912-13
CAGAGTA316500.022.73422210-11
AGAGTAC306750.021.8940610-11
Produced by FastQC (version 0.11.5)