##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431407_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3507256
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61117009992997	34.0	31.0	34.0	31.0	34.0
2	32.70670832126312	34.0	31.0	34.0	31.0	34.0
3	32.80242845118805	34.0	31.0	34.0	31.0	34.0
4	36.288258114035585	37.0	37.0	37.0	35.0	37.0
5	36.19816146868093	37.0	37.0	37.0	35.0	37.0
6	36.246378650432135	37.0	37.0	37.0	35.0	37.0
7	36.1951939065754	37.0	37.0	37.0	35.0	37.0
8	36.20658372243144	37.0	37.0	37.0	35.0	37.0
9	37.994548444710055	39.0	39.0	39.0	35.0	39.0
10-11	37.969930338703534	39.0	38.5	39.0	35.0	39.0
12-13	37.960515428585765	39.0	38.0	39.0	35.0	39.0
14-15	39.41253276065392	41.0	39.0	41.0	36.0	41.0
16-17	39.44569116140937	41.0	39.0	41.0	36.0	41.0
18-19	39.51435837589272	41.0	39.5	41.0	36.5	41.0
20-21	39.50854927612926	41.0	40.0	41.0	36.0	41.0
22-23	39.452842193441256	41.0	39.0	41.0	36.0	41.0
24-25	39.40846818709555	41.0	39.0	41.0	36.0	41.0
26-27	39.29261950082914	41.0	39.0	41.0	35.5	41.0
28-29	39.21027977427367	41.0	39.0	41.0	35.0	41.0
30-31	39.03165508876455	41.0	39.0	41.0	35.0	41.0
32-33	38.81131602597586	41.0	38.0	41.0	35.0	41.0
34-35	38.67924753710593	41.0	38.0	41.0	35.0	41.0
36-37	38.540673107409326	41.0	38.0	41.0	35.0	41.0
38-39	38.39445124621642	40.5	38.0	41.0	35.0	41.0
40-41	38.254385052017874	40.0	38.0	41.0	34.0	41.0
42-43	38.08326965582211	40.0	38.0	41.0	34.0	41.0
44-45	37.83943487444316	40.0	38.0	41.0	33.0	41.0
46-47	37.47024654031528	40.0	38.0	41.0	33.0	41.0
48-49	37.13905044285333	40.0	37.0	41.0	32.0	41.0
50-51	36.97604252441225	40.0	37.0	41.0	31.5	41.0
52-53	36.914068719249464	40.0	37.0	41.0	31.5	41.0
54-55	36.77194165467249	40.0	36.0	41.0	31.0	41.0
56-57	36.61634651134676	40.0	36.0	41.0	31.0	41.0
58-59	36.4307293222964	40.0	35.5	41.0	31.0	41.0
60-61	36.266897968098135	39.0	35.0	41.0	31.0	41.0
62-63	36.099243824802066	39.0	35.0	41.0	31.0	41.0
64-65	35.862020479828104	39.0	35.0	41.0	30.0	41.0
66-67	35.58987082779244	38.5	35.0	41.0	30.0	41.0
68-69	35.29306443555874	37.5	35.0	40.5	29.5	41.0
70-71	34.988233678978666	37.0	35.0	40.0	29.0	41.0
72-73	34.66531741623651	36.5	35.0	39.5	29.0	41.0
74-75	34.329314997251416	36.0	35.0	39.0	29.0	41.0
76-77	34.00141891552826	36.0	34.5	39.0	29.0	41.0
78-79	33.685585397815274	35.0	34.0	37.5	28.5	39.5
80-81	33.426030207090676	35.0	34.0	37.0	29.0	39.0
82-83	33.23230297417696	35.0	34.0	37.0	29.0	39.0
84-85	32.97681435287302	35.0	34.0	36.0	29.0	38.0
86-87	32.74231336406581	35.0	34.0	36.0	29.0	37.0
88-89	32.51484394067613	35.0	34.0	36.0	28.5	37.0
90-91	32.337726986567276	35.0	34.0	35.0	28.0	36.5
92-93	32.182569222206766	35.0	34.0	35.0	27.0	36.0
94-95	32.045266156790376	35.0	34.0	35.0	27.0	36.0
96-97	31.90541366241871	35.0	34.0	35.0	27.0	36.0
98-99	31.791752726347895	35.0	34.0	35.0	27.0	36.0
100	31.690059123143563	35.0	34.0	35.0	26.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.014957178502854163
1101	2	0.03898760113854394
1101	3	0.028077494028558192
1101	4	0.022676782066056944
1101	5	0.039836216402044045
1101	6	0.03977577163534818
1101	7	0.057831672466512885
1101	8	0.015439806411592372
1101	9	0.06063834536111301
1101	10-11	0.09191585246762912
1101	12-13	0.06141824501406745
1101	14-15	0.05641402125155537
1101	16-17	0.03454168855976292
1101	18-19	0.011772813692140005
1101	20-21	0.01667590268372976
1101	22-23	-0.01783427050827413
1101	24-25	0.009760212417909031
1101	26-27	0.04645869771885458
1101	28-29	-0.015068593827095356
1101	30-31	0.059247448389278645
1101	32-33	0.052656219115917224
1101	34-35	0.07338100964686589
1101	36-37	0.04202136148233393
1101	38-39	0.05032521925276967
1101	40-41	0.019264246858462286
1101	42-43	0.008875251294888642
1101	44-45	0.023620964289158053
1101	46-47	0.08719112638498672
1101	48-49	0.1121716748986259
1101	50-51	0.013879365324569903
1101	52-53	0.12180771752698405
1101	54-55	0.07903874116830423
1101	56-57	0.05401228582039863
1101	58-59	0.0527315942568265
1101	60-61	0.0756371283591264
1101	62-63	0.13327848572314593
1101	64-65	0.10922316847113933
1101	66-67	0.0561087317459652
1101	68-69	0.09061812763549426
1101	70-71	0.10818197129545837
1101	72-73	0.037627133072852814
1101	74-75	-0.05731474041964191
1101	76-77	-0.0460012993215102
1101	78-79	0.0020742276139102955
1101	80-81	-0.02989799144054217
1101	82-83	-0.006297059665982374
1101	84-85	-0.0036370689509084286
1101	86-87	0.02731544719149781
1101	88-89	0.07542141257648183
1101	90-91	0.04105187971184421
1101	92-93	0.0040156719249679895
1101	94-95	0.019450429530763813
1101	96-97	0.04923243746468131
1101	98-99	0.06935781594329882
1101	100	0.11750498884278215
1102	1	0.0015852918557541784
1102	2	-0.013007368094697824
1102	3	-0.02942901034263201
1102	4	0.05814584684648594
1102	5	0.013775106007955173
1102	6	-0.008445518295019383
1102	7	-0.017294197408034506
1102	8	0.017100400935369464
1102	9	0.0731446877977433
1102	10-11	0.07046528464129409
1102	12-13	0.02672237743882988
1102	14-15	0.04183977055334509
1102	16-17	0.041343725261384634
1102	18-19	0.046485881778288274
1102	20-21	0.06994812062797706
1102	22-23	0.061856159771970454
1102	24-25	0.051547383366497
1102	26-27	0.05255656081117621
1102	28-29	0.023385323546968095
1102	30-31	0.028439137972497974
1102	32-33	0.044427728334667904
1102	34-35	0.09053970760283647
1102	36-37	0.08686520823520993
1102	38-39	0.060347115060046974
1102	40-41	0.004839322375552513
1102	42-43	0.06319190681949038
1102	44-45	0.0655977033977777
1102	46-47	0.058843475942545354
1102	48-49	0.027299862612515824
1102	50-51	0.07763789287059808
1102	52-53	0.06825791475682053
1102	54-55	0.10580825480553102
1102	56-57	0.10161717711397955
1102	58-59	0.0871210953550019
1102	60-61	0.05150684580831211
1102	62-63	0.05729545034133565
1102	64-65	0.046289338957450354
1102	66-67	0.07306555532157688
1102	68-69	0.06953696797141617
1102	70-71	0.07446818441325576
1102	72-73	0.12990592040380022
1102	74-75	0.1583560005525868
1102	76-77	0.17959613041460898
1102	78-79	0.16056851664146876
1102	80-81	0.13393596860368007
1102	82-83	0.1818536694987145
1102	84-85	0.1654715264545672
1102	86-87	0.1638896588965153
1102	88-89	0.13463934764185126
1102	90-91	0.2028079817844315
1102	92-93	0.24291151352621654
1102	94-95	0.1761105680597268
1102	96-97	0.2488864816149743
1102	98-99	0.2490974751331052
1102	100	0.252508616660144
1103	1	-0.04614858074765493
1103	2	-0.05472384565891275
1103	3	-0.07326140711177942
1103	4	0.012704229990717408
1103	5	-0.008620231453413396
1103	6	-0.006852285418631254
1103	7	-0.02679871319838867
1103	8	0.006201418628677402
1103	9	0.013951922606914025
1103	10-11	0.03794073711514301
1103	12-13	0.0724303483755051
1103	14-15	-0.010035087768955009
1103	16-17	-0.05803545795920684
1103	18-19	-0.050800050669785435
1103	20-21	-0.042552386429065336
1103	22-23	-0.05129099414668303
1103	24-25	-0.026136618685590918
1103	26-27	0.004507418035835542
1103	28-29	-0.021688527819698322
1103	30-31	-0.0470607606160911
1103	32-33	-0.05984741871188248
1103	34-35	-0.03389805561942438
1103	36-37	-0.050090123022179966
1103	38-39	-0.07908784443504402
1103	40-41	-0.12991621647694984
1103	42-43	-0.06540342586958303
1103	44-45	-0.08975956302789001
1103	46-47	-0.11076793940278407
1103	48-49	-0.021891530541616078
1103	50-51	-0.12113139021039387
1103	52-53	-0.12076382307678557
1103	54-55	-0.0633766360877317
1103	56-57	-0.09799626645952486
1103	58-59	-0.10570207894137695
1103	60-61	-0.08584554093901176
1103	62-63	-0.11581799919753877
1103	64-65	-0.1240890449026324
1103	66-67	-0.09640764223977527
1103	68-69	-0.06371887511531327
1103	70-71	-0.028386546727212192
1103	72-73	-0.06356765894167893
1103	74-75	-0.013242780124357978
1103	76-77	-0.04116302345632761
1103	78-79	0.003567908173010892
1103	80-81	0.00272636911908819
1103	82-83	-0.04846112344304743
1103	84-85	-0.09247871037089794
1103	86-87	-0.08730812914171082
1103	88-89	-0.14810329406567746
1103	90-91	-0.16992642770290445
1103	92-93	-0.16016423483031872
1103	94-95	-0.028325345895673593
1103	96-97	-0.0556760176393567
1103	98-99	-0.07789335011714371
1103	100	-0.037868275855696965
1104	1	-0.11554068915083349
1104	2	-0.10053251591986623
1104	3	-0.09097953421346006
1104	4	-0.05468574998565856
1104	5	-0.0356836181146889
1104	6	-0.08597241840133307
1104	7	-0.015836318337100863
1104	8	-0.1064019757136947
1104	9	-0.0751524670535062
1104	10-11	-0.09529332826802062
1104	12-13	-0.06417208436356248
1104	14-15	-0.09085837264099439
1104	16-17	-0.04655737401240856
1104	18-19	-0.049102762215184725
1104	20-21	-0.05773199809378582
1104	22-23	-0.07217220975397964
1104	24-25	-0.0537084237732941
1104	26-27	-0.05043923936660377
1104	28-29	-0.028706238324339495
1104	30-31	-0.06353613272432312
1104	32-33	-0.011160446970706062
1104	34-35	-0.046963254082037054
1104	36-37	-0.06923142300428964
1104	38-39	-0.11486851679510579
1104	40-41	-0.12114633576853606
1104	42-43	-0.08613477208487552
1104	44-45	-0.0856338158064247
1104	46-47	-0.15914971936567213
1104	48-49	-0.19952663140735183
1104	50-51	-0.2613820520455903
1104	52-53	-0.22663763609772047
1104	54-55	-0.13290342758691764
1104	56-57	-0.2398444797243684
1104	58-59	-0.19922896425552494
1104	60-61	-0.2073302212498831
1104	62-63	-0.16065099391751403
1104	64-65	-0.19822871778160334
1104	66-67	-0.19278122512089624
1104	68-69	-0.21911549249981022
1104	70-71	-0.1872824033310465
1104	72-73	-0.18971799154501667
1104	74-75	-0.15280184983180334
1104	76-77	-0.1938676206823331
1104	78-79	-0.16250619977868297
1104	80-81	-0.14164892588497935
1104	82-83	-0.12586368048694396
1104	84-85	-0.12607372879674017
1104	86-87	-0.13513376231937002
1104	88-89	-0.12367845303805325
1104	90-91	-0.11060846243203315
1104	92-93	-0.11980738619195108
1104	94-95	-0.19873646062256967
1104	96-97	-0.20224212640021122
1104	98-99	-0.22265205511894948
1104	100	-0.18364405224774671
1105	1	-0.13479892375312374
1105	2	-0.13025766723426813
1105	3	-0.12620283352011086
1105	4	-0.024299532253962752
1105	5	-0.11607194802583365
1105	6	-0.014888810865926416
1105	7	-0.0222438091472128
1105	8	-0.005337239014778561
1105	9	-0.0053052851431587555
1105	10-11	-0.10763943655069141
1105	12-13	-0.07560942171812002
1105	14-15	-0.1062888538763076
1105	16-17	-0.13735524103142183
1105	18-19	-0.18557885463134483
1105	20-21	-0.07568304524677671
1105	22-23	-0.11176146786505115
1105	24-25	-0.11867862526758444
1105	26-27	-0.02711797595693355
1105	28-29	-0.02178366548540822
1105	30-31	-0.08914140939084803
1105	32-33	-0.1445735551106253
1105	34-35	-0.1360469578474195
1105	36-37	-0.06919697617875187
1105	38-39	-0.09397833762128727
1105	40-41	-0.1731100107045691
1105	42-43	-0.20776942970135082
1105	44-45	-0.18946920580777515
1105	46-47	-0.2238854651281983
1105	48-49	-0.20910984502509677
1105	50-51	-0.19104847790855217
1105	52-53	-0.22140274801730442
1105	54-55	-0.18232583419656123
1105	56-57	-0.14209786637918143
1105	58-59	-0.1789068431637446
1105	60-61	-0.15796307825244327
1105	62-63	-0.25713142430800673
1105	64-65	-0.24378667476573668
1105	66-67	-0.26183182180777465
1105	68-69	-0.15904158223190734
1105	70-71	-0.15010076291336105
1105	72-73	-0.08782090745068416
1105	74-75	-0.07222956205322362
1105	76-77	-0.14627438435370266
1105	78-79	-0.1602486650169368
1105	80-81	-0.09817989150327122
1105	82-83	-0.16611671196008615
1105	84-85	-0.16654554622270012
1105	86-87	-0.15381215791091307
1105	88-89	-0.14226839634928723
1105	90-91	-0.13909419523172062
1105	92-93	-0.16957216412792775
1105	94-95	-0.17933613048205643
1105	96-97	-0.17670534306193986
1105	98-99	-0.21828529566889188
1105	100	-0.20614169911208435
1106	1	-0.15545445063048646
1106	2	-0.08680146141888656
1106	3	-0.08518356217141587
1106	4	-0.04838819655219595
1106	5	-0.03277030641450551
1106	6	-0.06065066396734409
1106	7	-0.08924922455714324
1106	8	-0.06423660239862272
1106	9	-0.12688630468274198
1106	10-11	-0.08551940936933278
1106	12-13	-0.1046997401454206
1106	14-15	-0.06350946253618872
1106	16-17	-0.126569276391038
1106	18-19	-0.13131809385119197
1106	20-21	-0.10918906866089628
1106	22-23	-0.06089565796769136
1106	24-25	-0.013470391281352079
1106	26-27	-0.031042471671092642
1106	28-29	-0.06452434871522428
1106	30-31	-0.12446023159134256
1106	32-33	-0.06294288189500463
1106	34-35	-0.04475643168535015
1106	36-37	-0.037893142473855335
1106	38-39	0.012023393998511267
1106	40-41	-0.0277691781560776
1106	42-43	-0.0730475469150278
1106	44-45	-6.309259827901315E-4
1106	46-47	-0.0654985092025413
1106	48-49	-0.01553416191449486
1106	50-51	-0.021768225229159555
1106	52-53	0.03373317370513007
1106	54-55	-0.052730633853215636
1106	56-57	-0.0691520040861704
1106	58-59	0.01566593702485619
1106	60-61	-0.03307704178411086
1106	62-63	-0.003703428099093742
1106	64-65	0.04727476942305486
1106	66-67	0.06916115484963115
1106	68-69	-0.0019901865046207945
1106	70-71	0.0473676000055363
1106	72-73	0.0394326943780996
1106	74-75	-0.06484551501038993
1106	76-77	-0.016262000738819893
1106	78-79	0.009826538247210692
1106	80-81	-0.011367174678241554
1106	82-83	-0.1164450044220331
1106	84-85	-0.0561963983226903
1106	86-87	-0.07219836128235357
1106	88-89	-0.10119417364092698
1106	90-91	-0.12880726344740623
1106	92-93	-0.09592735779514783
1106	94-95	-0.07427181317539677
1106	96-97	-0.08251714788066522
1106	98-99	-0.07980347320611614
1106	100	-0.09559155962876886
1107	1	-0.24702839820630373
1107	2	-0.1886724784006546
1107	3	-0.19247737454870872
1107	4	-0.19797925688295948
1107	5	-0.2386607645864558
1107	6	-0.21022782100793336
1107	7	-0.17500135230935143
1107	8	-0.14243799960610914
1107	9	-0.12007610727797413
1107	10-11	-0.10496067272472231
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2216	30-31	-0.18028584856546104
2216	32-33	-0.0822637887445552
2216	34-35	-0.10513725710155342
2216	36-37	-0.023489236242681955
2216	38-39	-0.04805306019723332
2216	40-41	-0.16463134376935784
2216	42-43	-0.13246851881489619
2216	44-45	-0.13564020167564905
2216	46-47	-0.10665752911115334
2216	48-49	-0.11897098681672702
2216	50-51	-0.13011658973577767
2216	52-53	-0.13732120353849808
2216	54-55	-0.12423843587539807
2216	56-57	-0.17172719341470355
2216	58-59	-0.1614447047246017
2216	60-61	-0.23319581883821883
2216	62-63	-0.26998190768855324
2216	64-65	-0.23898652403416776
2216	66-67	-0.23824614845347725
2216	68-69	-0.24467226715094625
2216	70-71	-0.20305337992524386
2216	72-73	-0.19484641162178917
2216	74-75	-0.18340244206569878
2216	76-77	-0.21092883873141233
2216	78-79	-0.21072173149475049
2216	80-81	-0.26010841095543924
2216	82-83	-0.21787305957827385
2216	84-85	-0.1904212154188798
2216	86-87	-0.18589386156745746
2216	88-89	-0.17870676208124792
2216	90-91	-0.1741450833784981
2216	92-93	-0.18178999723169653
2216	94-95	-0.19518685425346405
2216	96-97	-0.18332063971725532
2216	98-99	-0.18700261550568342
2216	100	-0.20630558720688796
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43.0
3	211.0
4	71.0
5	104.0
6	255.0
7	619.0
8	1133.0
9	1917.0
10	2883.0
11	4341.0
12	6676.0
13	9952.0
14	11113.0
15	14981.0
16	18883.0
17	29061.0
18	33245.0
19	16733.0
20	10288.0
21	9522.0
22	9448.0
23	9310.0
24	10273.0
25	12240.0
26	15045.0
27	19176.0
28	24085.0
29	30356.0
30	38884.0
31	49285.0
32	62556.0
33	81188.0
34	113261.0
35	186791.0
36	302062.0
37	619476.0
38	1080311.0
39	665018.0
40	6460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83543439351544	20.784912856686226	14.60086414778963	22.778788602008703
2	14.61653678263798	21.898045195707112	40.36844654975916	23.11697147189575
3	16.425236476131413	28.470029724354724	31.821596846509703	23.283136953004156
4	11.131893423234574	18.492690582039064	39.55103362856889	30.824382366157476
5	11.385213692572046	39.10112545439194	35.5746104152452	13.939050437790806
6	27.541844678574932	39.91071652596788	18.513247963650215	14.034190831806976
7	24.33845718704309	35.01671962354615	23.607230267764884	17.037592921645867
8	25.011718562887907	35.05401373609454	21.101140036541388	18.83312766447616
9	25.963296081978644	15.65959703528828	22.01105993148488	36.3660469512482
10-11	22.92520135399298	27.384057508205846	29.587418198158332	20.103322939642844
12-13	24.607998389624253	25.788921595686197	29.588387616986044	20.014692397703506
14-15	21.874322832436526	26.719649207243496	28.407207800057936	22.99882016026204
16-17	19.581604536423917	29.917605102108315	28.980105814916275	21.52068454655149
18-19	19.477388863196108	27.613283782697234	33.01209436214201	19.897232991964653
20-21	21.087146500610878	26.623898176779274	32.8338543961018	19.455100926508052
22-23	20.591468509476147	26.714079834833726	32.917666202343234	19.7767854533469
24-25	20.28047864789966	26.85922849339847	32.85425964953498	20.006033209166883
26-27	20.008875897390862	26.899586029058085	32.89720353633088	20.194334537220175
28-29	19.968776133344242	26.863089146976282	33.1051252932138	20.06300942646567
30-31	20.231654826410708	27.169623784373144	32.71246597043149	19.886255418784664
32-33	19.96778919759119	26.994887814625223	32.990596367452916	20.04672662033067
34-35	20.268357997706442	26.87393540977493	32.73035779355603	20.127348798962597
36-37	20.41994627924386	27.02801842090744	32.247294537924084	20.304740761924613
38-39	20.309906244885553	27.17760666419177	32.2991387485594	20.213348342363272
40-41	20.271252599082327	27.008843829812413	32.331864232564136	20.38803933854112
42-43	20.265115091490255	27.109929869185994	32.129872236834096	20.495082802489655
44-45	20.588518321413495	27.179682306514636	31.742489874897657	20.48930949717421
46-47	20.7046099282186	27.462705419395363	31.163757997551066	20.66892665483497
48-49	20.812756317234825	27.514587281404623	30.683223225714844	20.989433175645708
50-51	20.827239397549672	27.67130368906522	30.437977859250438	21.063479054134678
52-53	20.813999440013276	27.8282180846059	30.338749199509813	21.019033275871017
54-55	20.932065321214342	28.03495187929364	30.223892299945653	20.809090499546365
56-57	20.837579387188217	27.69140887631024	30.24253402593196	21.228477710569578
58-59	20.810308874766	27.43978475324048	30.529601632969378	21.22030473902414
60-61	20.89036286815405	27.686618752994725	30.368579245528604	21.05443913332262
62-63	20.721182317697842	27.603342026845816	30.54048898655428	21.13498666890206
64-65	20.78798013797351	27.51121622835343	30.580777515935097	21.120026117737964
66-67	20.69275074342741	27.540694087978977	30.41991334770648	21.346641820887136
68-69	21.06381820027349	27.689804302181425	30.152492954582	21.093884542963078
70-71	20.995222604177552	27.542611431107407	30.22545755636439	21.236708408350655
72-73	20.92616490194021	27.64247067786481	30.23461699885494	21.19674742134004
74-75	20.918528166312424	27.798272739701595	30.005783765851717	21.277415328134264
76-77	20.95530769901911	27.691756961199538	29.91801782947549	21.434917510305866
78-79	21.027829401355945	27.744861608576123	30.01002786817844	21.217281121889496
80-81	20.889848812095032	27.726165273114784	30.026530946831187	21.357454967959
82-83	20.817561017335766	27.685290260036492	30.19458542427007	21.30256329835766
84-85	20.77563318099961	27.67624255391462	29.93480291467981	21.613321350405965
86-87	20.88668211874645	27.68666170352479	29.888868201210826	21.537787976517933
88-89	20.76279951671021	27.59256817201695	29.9748589513752	21.66977335989764
90-91	20.692094966356553	27.610948953601728	29.891957193458868	21.80499888658285
92-93	20.662780008736284	27.643823641114366	29.948104078111047	21.745292272038302
94-95	20.501629705620832	27.472361723794158	30.08382544792847	21.942183122656537
96-97	20.556968976557425	27.620064831402143	29.875264131366702	21.94770206067373
98-99	20.443564931200946	27.578043494562536	29.869815122590595	22.108576451645924
100	20.491599251492694	27.545479403075973	29.773637271626434	22.189284073804895
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2989.0
1	1706.0
2	2268.0
3	3992.5
4	2385.5
5	1280.5
6	3037.0
7	7750.0
8	13855.0
9	15401.5
10	9800.5
11	4519.0
12	3678.5
13	3919.5
14	4352.0
15	4820.5
16	5184.0
17	5786.5
18	6591.0
19	7258.5
20	8370.0
21	10125.0
22	12716.5
23	17034.5
24	22208.0
25	27234.0
26	33999.5
27	42932.0
28	54060.0
29	65324.0
30	72892.5
31	79631.5
32	87379.0
33	95145.0
34	101999.0
35	107491.5
36	111188.0
37	114191.0
38	115907.0
39	116166.5
40	117272.5
41	117258.0
42	115812.5
43	115357.0
44	114442.0
45	110892.0
46	107903.0
47	105931.0
48	102928.5
49	100611.0
50	99847.0
51	96056.5
52	89027.0
53	83100.5
54	78905.5
55	75649.0
56	69174.0
57	60859.5
58	55526.5
59	51544.0
60	45669.5
61	38048.5
62	31958.0
63	26485.5
64	20931.5
65	16883.0
66	13536.0
67	10298.0
68	7765.0
69	5914.0
70	4308.5
71	3109.0
72	2341.0
73	1826.0
74	1529.5
75	1155.0
76	742.0
77	581.0
78	461.5
79	265.0
80	124.0
81	56.0
82	31.0
83	20.0
84	13.0
85	9.5
86	6.5
87	2.5
88	1.5
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	1.5
95	1.5
96	0.5
97	1.0
98	1.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006187173106268832
2	0.004362384724696458
3	8.83881872324119E-4
4	0.0
5	0.04197013277616461
6	0.0
7	0.0
8	0.0
9	2.8512318462068352E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.7024636924136704E-5
20-21	2.8512318462068352E-5
22-23	7.128079615517088E-5
24-25	4.276847769310253E-5
26-27	1.4256159231034176E-4
28-29	8.553695538620506E-5
30-31	3.4214782154482024E-4
32-33	0.0013543351269482468
34-35	0.0010834681015585972
36-37	0.00129731049002411
38-39	9.694188277103239E-4
40-41	2.4235470692758097E-4
42-43	6.985518023206747E-4
44-45	2.1384238846551266E-4
46-47	2.9937934385171767E-4
48-49	1.5681775154137594E-4
50-51	3.564039807758544E-4
52-53	8.553695538620507E-4
54-55	9.694188277103239E-4
56-57	0.0022239608400413317
58-59	7.27064120782743E-4
60-61	0.00129731049002411
62-63	0.001226029693868939
64-65	0.0017392514261861695
66-67	5.845025284724012E-4
68-69	3.4214782154482024E-4
70-71	2.9937934385171767E-4
72-73	2.2809854769654682E-4
74-75	7.27064120782743E-4
76-77	8.553695538620507E-4
78-79	8.553695538620507E-4
80-81	8.83881872324119E-4
82-83	0.0015966898338758276
84-85	0.0012545420123310075
86-87	0.0019673499738827164
88-89	0.0012402858530999733
90-91	9.979311461723924E-4
92-93	0.0012545420123310075
94-95	7.840887577068797E-4
96-97	1.4256159231034176E-4
98-99	3.7066014000688855E-4
100	2.5661086615861515E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3507256.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.30550393823651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.10977011581177	41.16482449179405
2	16.167959684365606	18.20690235362593
3	5.133363558835198	8.67109866235185
4	2.0427050153589406	4.600621411477932
5	1.053960244896738	2.9671881359889003
6	0.59766063208408	2.019094984412346
7	0.3746775905312705	1.476748738445919
8	0.264184541441855	1.1900034990860457
9	0.18430860765166532	0.9339830130583556
>10	0.9658732473327462	9.835965107515396
>50	0.07120876727350468	2.7472391700257606
>100	0.032045293097366284	3.0346366260526683
>500	0.001471074189152913	0.5377067409247459
>1k	6.087203475338165E-4	0.7406963129684178
>5k	5.072669562781802E-5	0.14886473540243675
>10k+	1.5218008688345404E-4	1.7244260168691712
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25825	0.7363306242829152	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20850	0.5944818399341251	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13700	0.3906187629303364	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5212	0.14860620382430026	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4228	0.120550082457625	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4170	0.11889636798682504	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3810	0.10863193334048044	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3735	0.10649350945582528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.8512318462068352E-5	0.0
3	0.0	0.0	0.0	2.8512318462068352E-5	0.0
4	0.0	0.0	0.0	2.8512318462068352E-5	0.0
5	0.0	0.0	0.0	2.8512318462068352E-5	0.0
6	0.0	0.0	0.0	2.8512318462068352E-5	0.0
7	0.0	0.0	0.0	2.8512318462068352E-5	0.0
8	0.0	0.0	0.0	2.8512318462068352E-5	0.0
9	0.0	0.0	0.0	2.8512318462068352E-5	0.0
10-11	0.0	0.0	0.0	2.8512318462068352E-5	0.0
12-13	0.0	0.0	0.0	5.7024636924136704E-5	0.0
14-15	0.0	0.0	0.0	8.553695538620506E-5	0.0
16-17	0.0	0.0	0.0	9.979311461723924E-5	0.0
18-19	0.0	0.0	0.0	1.2830543307930758E-4	0.0
20-21	0.0	0.0	0.0	1.9958622923447848E-4	0.0
22-23	0.0	0.0	0.0	2.993793438517177E-4	0.0
24-25	0.0	0.0	0.0	5.274778915482645E-4	0.0
26-27	0.0	0.0	0.0	9.83674986941358E-4	0.0
28-29	0.0	0.0	0.0	0.0022524731585033997	0.0
30-31	0.0	0.0	0.0	0.006343990857810208	0.0
32-33	0.0	0.0	0.0	0.014013804524106596	0.0
34-35	0.0	0.0	0.0	0.02434951996660637	0.0
36-37	0.0	0.0	0.0	0.041542447999233595	0.0
38-39	0.0	0.0	0.0	0.06532172159659859	0.0
40-41	0.0	0.0	0.0	0.09329230600788765	0.0
42-43	0.0	0.0	0.0	0.119723225222225	0.0
44-45	0.0	0.0	0.0	0.15098698241588296	0.0
46-47	0.0	0.0	0.0	0.1919591840458752	0.0
48-49	0.0	0.0	0.0	0.23784976061057417	0.0
50-51	0.0	0.0	0.0	0.28489508607298697	0.0
52-53	0.0	0.0	0.0	0.331199091255386	0.0
54-55	0.0	0.0	0.0	0.38236444673556763	0.0
56-57	0.0	0.0	0.0	0.4476291436952421	0.0
58-59	0.0	0.0	0.0	0.5217611716966197	0.0
60-61	0.0	0.0	0.0	0.6020376043265733	0.0
62-63	0.0	0.0	0.0	0.6841673376565611	0.0
64-65	0.0	0.0	0.0	0.7724272194558937	0.0
66-67	0.0	0.0	0.0	0.8763688764093639	0.0
68-69	0.0	0.0	0.0	0.999085895070106	0.0
70-71	0.0	0.0	0.0	1.1354032896372548	1.4256159231034176E-5
72-73	0.0	0.0	0.0	1.2795758279407035	2.8512318462068352E-5
74-75	0.0	0.0	0.0	1.423648573129535	2.8512318462068352E-5
76-77	0.0	0.0	0.0	1.5827045416701833	2.8512318462068352E-5
78-79	0.0	0.0	0.0	1.7698736562144308	2.8512318462068352E-5
80-81	0.0	0.0	0.0	1.9842150102530298	2.8512318462068352E-5
82-83	0.0	0.0	0.0	2.2107311242749317	2.8512318462068352E-5
84-85	0.0	0.0	0.0	2.432870597412906	2.8512318462068352E-5
86-87	0.0	0.0	0.0	2.6733434913219907	2.8512318462068352E-5
88	0.0	0.0	0.0	2.872416498824152	2.8512318462068352E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9505	0.0	65.208176	1
GTATCAA	19140	0.0	45.4782	1
ATCAACG	24380	0.0	35.369587	3
TCAACGC	24660	0.0	35.006123	4
TATCAAC	24850	0.0	34.91025	2
CAACGCA	25460	0.0	33.832294	5
AACGCAG	26440	0.0	32.724346	6
GTGGTAT	2975	0.0	32.720665	1
TGGTATC	3155	0.0	30.402815	2
ACGCAGA	29695	0.0	28.979015	7
CGCAGAG	29770	0.0	28.811287	8
CATGGGT	7445	0.0	28.040302	4
GCAGAGT	31950	0.0	26.58067	9
GTACATG	17870	0.0	26.473549	1
TACATGG	18020	0.0	25.780132	2
ACATGGG	17770	0.0	25.295012	3
TATAACG	340	0.0	24.892962	2
GAGTACT	22490	0.0	23.133259	12-13
CAGAGTA	31650	0.0	22.734222	10-11
AGAGTAC	30675	0.0	21.89406	10-11
>>END_MODULE