FastQCFastQC Report
Wed 25 May 2016
SRR2431406_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431406_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4374582
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA153200.35020488814702755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54050.049.792731
GTATCAA94450.038.457491
ATCAACG105100.033.395413
TCAACGC105700.033.0255284
TATCAAC114400.031.0095882
CAACGCA113250.030.7819275
AACGCAG122700.028.5344986
GTACATG197950.026.811381
TACATGG198000.025.924492
CGCAGAG134400.025.4554468
ACGCAGA141050.024.5554547
ACATGGG206600.024.2077053
CATGGGT79600.023.2273854
GCAGAGT154350.021.5875369
CAGAGTA150500.018.90780610-11
CATGGGG101800.018.624224
TACCTGG30350.018.6025812
TGGTATC26050.018.2416572
ATGGGTG50300.018.238225
GTGGTAT28500.018.1598971
Produced by FastQC (version 0.11.5)