##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431406_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4374582
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.045667220319565	33.0	31.0	34.0	27.0	34.0
2	31.027704589832812	33.0	31.0	34.0	27.0	34.0
3	31.173769745315095	34.0	31.0	34.0	27.0	34.0
4	34.43699123710562	37.0	35.0	37.0	31.0	37.0
5	34.474740900959226	37.0	35.0	37.0	31.0	37.0
6	34.536057387883005	37.0	35.0	37.0	31.0	37.0
7	34.387973525241954	37.0	35.0	37.0	31.0	37.0
8	34.38378958263898	37.0	35.0	37.0	31.0	37.0
9	35.86896919522825	39.0	37.0	39.0	30.0	39.0
10-11	35.89578398576138	39.0	37.0	39.0	30.5	39.0
12-13	35.84396429190263	39.0	37.0	39.0	30.0	39.0
14-15	37.21892000195676	41.0	38.0	41.0	31.0	41.0
16-17	37.24560049394434	41.0	38.0	41.0	31.0	41.0
18-19	37.275334420522924	41.0	38.0	41.0	31.0	41.0
20-21	37.366452726226186	41.0	38.0	41.0	31.0	41.0
22-23	37.33493417199632	41.0	38.0	41.0	30.5	41.0
24-25	37.37588082701387	41.0	38.0	41.0	30.5	41.0
26-27	37.41593231078992	41.0	38.0	41.0	30.0	41.0
28-29	37.38356864724447	40.0	38.0	41.0	30.0	41.0
30-31	37.31847991419523	40.0	38.0	41.0	30.0	41.0
32-33	37.27020341600638	40.0	38.0	41.0	30.0	41.0
34-35	37.19989944182096	40.0	38.0	41.0	30.0	41.0
36-37	37.06436854995518	40.0	38.0	41.0	30.0	41.0
38-39	36.94340510704794	40.0	38.0	41.0	30.0	41.0
40-41	36.88506399011379	40.0	37.5	41.0	30.0	41.0
42-43	36.72157717011591	40.0	37.0	41.0	29.5	41.0
44-45	36.567000801448	40.0	37.0	41.0	29.0	41.0
46-47	36.439918716805394	40.0	37.0	41.0	29.0	41.0
48-49	36.30840660890571	40.0	36.0	41.0	29.0	41.0
50-51	36.178262860314426	40.0	36.0	41.0	29.0	41.0
52-53	36.02753245910124	40.0	35.5	41.0	28.0	41.0
54-55	35.86935734659906	40.0	35.0	41.0	28.0	41.0
56-57	35.683088007037014	39.0	35.0	41.0	28.0	41.0
58-59	35.47860058401008	39.0	35.0	41.0	28.0	41.0
60-61	35.24962042087678	39.0	35.0	41.0	27.0	41.0
62-63	35.152507713879864	39.0	35.0	41.0	27.5	41.0
64-65	34.90485513815949	38.0	34.0	40.5	26.5	41.0
66-67	34.64379316698144	38.0	34.0	40.0	26.0	41.0
68-69	34.34841706018998	37.0	34.0	40.0	26.0	41.0
70-71	34.013117138963224	37.0	34.0	40.0	26.0	41.0
72-73	33.66849472703906	36.0	34.0	39.0	25.5	41.0
74-75	33.382832576918204	36.0	33.5	39.0	25.0	41.0
76-77	33.10880479552104	35.5	33.0	39.0	25.0	40.5
78-79	32.75534256758703	35.0	33.0	37.5	24.0	39.5
80-81	32.42541504536891	35.0	33.0	37.0	24.0	39.0
82-83	32.090118324447914	35.0	33.0	37.0	23.5	39.0
84-85	31.780877578703517	35.0	33.0	36.0	22.5	38.0
86-87	31.494488844877065	35.0	32.5	36.0	21.5	37.0
88-89	31.221753758416234	35.0	32.0	36.0	20.0	37.0
90-91	30.986736789937872	35.0	32.0	35.0	19.5	36.5
92-93	30.754099591686703	35.0	32.0	35.0	18.0	36.0
94-95	30.541094326269345	35.0	32.0	35.0	13.5	36.0
96-97	30.338689387923235	35.0	32.0	35.0	3.5	36.0
98-99	30.128956206558705	35.0	31.5	35.0	2.0	35.5
100	29.960497482959514	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1791812849858907
1101	2	0.1954419470900497
1101	3	0.19910611815602763
1101	4	0.33579080119067584
1101	5	0.22543706541382136
1101	6	0.30835062697606475
1101	7	0.2971145897448153
1101	8	0.2904458471880389
1101	9	0.2265740039265154
1101	10-11	0.2984510826743687
1101	12-13	0.241819616910405
1101	14-15	0.2945576756390267
1101	16-17	0.259356279592609
1101	18-19	0.15207763935335805
1101	20-21	0.1965767872263271
1101	22-23	0.2732725014783952
1101	24-25	0.24425517892116488
1101	26-27	0.19731250875635453
1101	28-29	0.2570195026454343
1101	30-31	0.2771676965165213
1101	32-33	0.28665777667663406
1101	34-35	0.2121426711846226
1101	36-37	0.1789444919531462
1101	38-39	0.28194838819086243
1101	40-41	0.1917764925489891
1101	42-43	0.22844755550339357
1101	44-45	0.20232151926617092
1101	46-47	0.23396951721269943
1101	48-49	0.30061591114489516
1101	50-51	0.2413949703747491
1101	52-53	0.24224149992116395
1101	54-55	0.27403169110534265
1101	56-57	0.16096928080745698
1101	58-59	0.18084627478651782
1101	60-61	0.25341235773403525
1101	62-63	0.2438507357453048
1101	64-65	0.27628549764314414
1101	66-67	0.3054111826640806
1101	68-69	0.2852167953058071
1101	70-71	0.2685069289625943
1101	72-73	0.22877843097722206
1101	74-75	0.29985818319331514
1101	76-77	0.3010435825238673
1101	78-79	0.31700398694708554
1101	80-81	0.2394622187136335
1101	82-83	0.23161412731080588
1101	84-85	0.19480047890327512
1101	86-87	0.21030757623417884
1101	88-89	0.21893546437655687
1101	90-91	0.16172805681728164
1101	92-93	0.15997240609913277
1101	94-95	0.09805675372458467
1101	96-97	0.14730824490414918
1101	98-99	0.11290800624328767
1101	100	0.11998697936000724
1102	1	0.17323637639755773
1102	2	0.1936587515267547
1102	3	0.16816205022582054
1102	4	0.229433530231411
1102	5	0.24120509311904215
1102	6	0.21143573587213638
1102	7	0.1333272231321203
1102	8	0.1832873411807867
1102	9	0.23831115604762232
1102	10-11	0.27621806691188056
1102	12-13	0.21970935903701871
1102	14-15	0.23695379267309846
1102	16-17	0.16012228779461424
1102	18-19	0.18443653940675375
1102	20-21	0.18400578688213187
1102	22-23	0.15119634700854334
1102	24-25	0.20363137185737656
1102	26-27	0.23564754550716316
1102	28-29	0.2371288900304762
1102	30-31	0.23596454711235282
1102	32-33	0.22149934602722965
1102	34-35	0.22284784651717615
1102	36-37	0.21983005084690888
1102	38-39	0.16133077237221727
1102	40-41	0.20415104436149534
1102	42-43	0.16592857336584643
1102	44-45	0.1485074334473282
1102	46-47	0.10528218609617568
1102	48-49	0.08073239571085367
1102	50-51	0.032448898151407946
1102	52-53	0.05712887443129233
1102	54-55	0.06760313028461695
1102	56-57	0.04931785144881218
1102	58-59	0.033059229406553925
1102	60-61	0.027336071016030417
1102	62-63	0.11325646367560438
1102	64-65	0.12937631480670575
1102	66-67	0.11276129716672045
1102	68-69	0.08540252753032007
1102	70-71	0.03944228534046346
1102	72-73	0.06161810796853473
1102	74-75	0.08028837239000097
1102	76-77	0.07960415060203019
1102	78-79	0.05713024238440312
1102	80-81	0.05104883980684605
1102	82-83	0.04009381445824545
1102	84-85	0.06799959489257645
1102	86-87	0.03547891210804721
1102	88-89	0.03969047874520726
1102	90-91	0.05198500477657575
1102	92-93	-0.003207237144280839
1102	94-95	0.025662058435134583
1102	96-97	0.03511683216476058
1102	98-99	0.028426763102409325
1102	100	-0.045166729240207815
1103	1	-0.15803703188978702
1103	2	-0.09706474185156466
1103	3	-0.012640947052641849
1103	4	0.0178904920573828
1103	5	0.02827821029831057
1103	6	0.06476280706589677
1103	7	-0.059510836361084785
1103	8	0.02992818754660931
1103	9	0.050300117245683396
1103	10-11	0.019890397066397725
1103	12-13	0.06714166979450198
1103	14-15	0.027019608743785284
1103	16-17	0.0583053302022023
1103	18-19	-0.0038982877996645016
1103	20-21	-0.061463134494012195
1103	22-23	-0.02880340620345123
1103	24-25	0.0809869824299767
1103	26-27	0.049619766337137605
1103	28-29	-0.011335859610724697
1103	30-31	0.03006983756021242
1103	32-33	0.014452098336796837
1103	34-35	0.021562589861474635
1103	36-37	-0.04951025428991329
1103	38-39	0.010285332594726526
1103	40-41	0.029115978839278966
1103	42-43	-0.06490707879943614
1103	44-45	-0.14698062035957804
1103	46-47	-0.2533825025349614
1103	48-49	-0.19030128220128262
1103	50-51	-0.24561887390502335
1103	52-53	-0.285624882788845
1103	54-55	-0.26644628685046
1103	56-57	-0.2629434797460206
1103	58-59	-0.2823690406426209
1103	60-61	-0.25577312198974056
1103	62-63	-0.1712757420316109
1103	64-65	-0.2393301267926944
1103	66-67	-0.1911454841350917
1103	68-69	-0.17823911186457053
1103	70-71	-0.1590233038887945
1103	72-73	-0.15661605263041167
1103	74-75	-0.23474711038203822
1103	76-77	-0.20363334672337885
1103	78-79	-0.1850037716428261
1103	80-81	-0.11444177601573102
1103	82-83	-0.17972247349907988
1103	84-85	-0.2656460185524345
1103	86-87	-0.2213939811301877
1103	88-89	-0.2502279387381705
1103	90-91	-0.30132232337692244
1103	92-93	-0.32912296692820675
1103	94-95	-0.37860582205535565
1103	96-97	-0.3490343023349567
1103	98-99	-0.38183886729210315
1103	100	-0.3091473841137997
1104	1	-0.09606509196952828
1104	2	-0.07962491337307753
1104	3	-0.06860089523257429
1104	4	0.015123030218681777
1104	5	-0.030113509338953293
1104	6	-0.010280817867908354
1104	7	-0.035320637927590326
1104	8	-0.047622618645185355
1104	9	-0.08516611897658777
1104	10-11	-0.08578569084490795
1104	12-13	-0.08123700693550262
1104	14-15	-0.07284654702127824
1104	16-17	-0.08047003522646179
1104	18-19	-0.09766781355161669
1104	20-21	-0.1624479443033593
1104	22-23	-0.15973630303215458
1104	24-25	-0.1565298202949208
1104	26-27	-0.16191225796777076
1104	28-29	-0.1478253262201079
1104	30-31	-0.1440490693567753
1104	32-33	-0.15379362059382373
1104	34-35	-0.1301934188783278
1104	36-37	-0.1281867582985896
1104	38-39	-0.10878730765984557
1104	40-41	-0.06713237729988464
1104	42-43	-0.19074433977887395
1104	44-45	-0.1967234661592201
1104	46-47	-0.2791617559754229
1104	48-49	-0.3363352866726359
1104	50-51	-0.34757505887724705
1104	52-53	-0.3980085381226104
1104	54-55	-0.31170294007662136
1104	56-57	-0.39262607555075135
1104	58-59	-0.42461294300814956
1104	60-61	-0.478083348819311
1104	62-63	-0.36474521060470977
1104	64-65	-0.3189024831618852
1104	66-67	-0.27060508991987575
1104	68-69	-0.2901360814352216
1104	70-71	-0.36190098005153715
1104	72-73	-0.3723541634077421
1104	74-75	-0.3265960592527861
1104	76-77	-0.3120024032420545
1104	78-79	-0.36216896581363756
1104	80-81	-0.3385465451645615
1104	82-83	-0.35733658575396277
1104	84-85	-0.37292349121167234
1104	86-87	-0.3886075299837124
1104	88-89	-0.4085990795104131
1104	90-91	-0.42922608409858753
1104	92-93	-0.41911600100123536
1104	94-95	-0.44542171792906515
1104	96-97	-0.5256295033787914
1104	98-99	-0.5249615838730435
1104	100	-0.48576695585745
1105	1	-0.20114999345529938
1105	2	-0.22307550104560647
1105	3	-0.1082319411201027
1105	4	-0.04438357247136082
1105	5	-0.10196824300435026
1105	6	-0.10547706976105076
1105	7	-0.1922426141260658
1105	8	-0.10864149629642839
1105	9	-0.20541619383508447
1105	10-11	-0.21281751043514419
1105	12-13	-0.18614414987295191
1105	14-15	-0.22946922633002487
1105	16-17	-0.24956513815605064
1105	18-19	-0.2175423683358062
1105	20-21	-0.21952689589408436
1105	22-23	-0.224717053936196
1105	24-25	-0.21750624925965667
1105	26-27	-0.19648601520270859
1105	28-29	-0.21042286877765548
1105	30-31	-0.1882136000668524
1105	32-33	-0.20082875930742716
1105	34-35	-0.29759928592490326
1105	36-37	-0.3360115268304895
1105	38-39	-0.37503310918439325
1105	40-41	-0.4031474556316752
1105	42-43	-0.33224557808158295
1105	44-45	-0.3783906710991616
1105	46-47	-0.3974071710499274
1105	48-49	-0.567077796953356
1105	50-51	-0.46119819086397484
1105	52-53	-0.38951078716768706
1105	54-55	-0.45810787407464915
1105	56-57	-0.4741378355443331
1105	58-59	-0.4315682836748138
1105	60-61	-0.5446660827425447
1105	62-63	-0.46187403678486305
1105	64-65	-0.501562882677554
1105	66-67	-0.5631086568682946
1105	68-69	-0.4794100137267563
1105	70-71	-0.49943036230698823
1105	72-73	-0.533931013215188
1105	74-75	-0.5591862589104153
1105	76-77	-0.5110274693285248
1105	78-79	-0.5330780833253925
1105	80-81	-0.5359117006056415
1105	82-83	-0.4622244610257127
1105	84-85	-0.4567459253898498
1105	86-87	-0.4803024631634223
1105	88-89	-0.474480363221506
1105	90-91	-0.5375862519560961
1105	92-93	-0.5047504307694375
1105	94-95	-0.5117237371679622
1105	96-97	-0.5034528563728706
1105	98-99	-0.5616612401234313
1105	100	-0.5059961353936338
1106	1	-0.1414002696875336
1106	2	-0.12196705081477432
1106	3	-0.12241527556943765
1106	4	-0.06164220724759417
1106	5	-0.06806069220743893
1106	6	-0.053193959111141
1106	7	-0.04822660299249293
1106	8	-0.0587465860824139
1106	9	0.004873766826555936
1106	10-11	-0.048333934514495525
1106	12-13	-0.06382844584287284
1106	14-15	-0.060672724490508756
1106	16-17	-0.07200601641182658
1106	18-19	-0.11105979461355986
1106	20-21	-0.11731575842458852
1106	22-23	-0.06492567570693097
1106	24-25	-0.08267132929799459
1106	26-27	-0.14405263780357558
1106	28-29	-0.1252149695620659
1106	30-31	-0.07291280010877443
1106	32-33	-0.09840732593980306
1106	34-35	-0.06625484649621427
1106	36-37	-0.1271504392491707
1106	38-39	-0.08547884997087607
1106	40-41	-0.04685551747366645
1106	42-43	-0.10038245724004469
1106	44-45	-0.1261525617115069
1106	46-47	-0.08097596914397798
1106	48-49	-0.06510162137625741
1106	50-51	-0.04197266963974755
1106	52-53	-0.15395611601412185
1106	54-55	-0.09571260488535671
1106	56-57	-0.11115824547919573
1106	58-59	-0.1982417069529916
1106	60-61	-0.163024903744855
1106	62-63	-0.10209267714669323
1106	64-65	0.004501866841792435
1106	66-67	0.09340349884048038
1106	68-69	0.005260923903591674
1106	70-71	-0.020114596696750198
1106	72-73	-0.07984857426649228
1106	74-75	-0.025800515792084866
1106	76-77	-0.037942072617404676
1106	78-79	0.024562271404462876
1106	80-81	-0.017131786150450523
1106	82-83	-0.03860429475054161
1106	84-85	-0.022477249702429702
1106	86-87	-0.028593477885539897
1106	88-89	-0.03946361334406845
1106	90-91	-0.024371890240317384
1106	92-93	-0.008869213163091416
1106	94-95	-0.017905854942537047
1106	96-97	-0.003495678942964986
1106	98-99	-0.028702377684769687
1106	100	-0.02197240747297613
1107	1	-0.1678107896019334
1107	2	-0.2771011747705465
1107	3	-0.22498173430242474
1107	4	-0.10395891483818787
1107	5	-0.13246683008509308
1107	6	-0.1946970508616772
1107	7	-0.2423547357807081
1107	8	-0.1821375521922235
1107	9	-0.22300657015509273
1107	10-11	-0.23680966845677887
1107	12-13	-0.15814485212820983
1107	14-15	-0.21578370545788061
1107	16-17	-0.24044391661958997
1107	18-19	-0.21642395869927356
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2216	54-55	0.5275666033214819
2216	56-57	0.5365730845530194
2216	58-59	0.5164253737608746
2216	60-61	0.563319752233717
2216	62-63	0.4973335879844498
2216	64-65	0.5415604468177833
2216	66-67	0.47796036154151267
2216	68-69	0.4420524035424549
2216	70-71	0.400975753091501
2216	72-73	0.4529316766945257
2216	74-75	0.45316920668012983
2216	76-77	0.4539428598239823
2216	78-79	0.5496186446459319
2216	80-81	0.5164786368690741
2216	82-83	0.46029403526439694
2216	84-85	0.5229176677313454
2216	86-87	0.4427352555782136
2216	88-89	0.44969684656208386
2216	90-91	0.5059486545207079
2216	92-93	0.5003898509389764
2216	94-95	0.4895343753829806
2216	96-97	0.5057824963577566
2216	98-99	0.49070176985383895
2216	100	0.5791558570922923
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91552.0
3	18512.0
4	12218.0
5	10226.0
6	7508.0
7	6659.0
8	7492.0
9	8232.0
10	8441.0
11	9213.0
12	10660.0
13	12006.0
14	13211.0
15	14067.0
16	13974.0
17	13730.0
18	13005.0
19	12349.0
20	11734.0
21	12447.0
22	14576.0
23	20468.0
24	32002.0
25	43180.0
26	47835.0
27	38526.0
28	37711.0
29	47451.0
30	56282.0
31	64126.0
32	81957.0
33	109713.0
34	155684.0
35	242150.0
36	410817.0
37	763417.0
38	1234237.0
39	664556.0
40	2658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.86982802488548	21.699794941649746	14.147766595321446	22.282610438143326
2	14.683818004737137	22.45534631637596	40.52566964209039	22.335166036796508
3	16.96135467848854	28.63094497821075	31.399635788383478	23.008064554917233
4	11.530233022551101	19.090435247798297	40.337254294858994	29.042077434791608
5	11.68838635009685	40.50520738734162	35.400545440927864	12.40586082163367
6	27.204785925900506	40.86366288047768	19.21805474531874	12.713496448303077
7	24.474443146519004	35.396851760595446	23.78033519420152	16.34836989868403
8	23.567909946736176	37.634180346712384	21.529336278074453	17.268573428476987
9	24.74213740381189	18.308004159288107	22.064973094090398	34.88488534280961
10-11	21.928636986295103	29.228197024032298	30.012348774410448	18.83081721526215
12-13	22.82625877794892	27.391890281271824	29.387441742465608	20.394409198313646
14-15	20.70658563598346	29.032452877839415	26.784790470762378	23.47617101541475
16-17	19.87774100270815	32.06384999980557	27.531915506856606	20.526493490629676
18-19	19.93252072869286	30.970578987383153	29.307136485393237	19.789763798530753
20-21	21.720040619244454	29.956578402851125	28.484428183935904	19.838952793968513
22-23	21.298622956489872	30.02242629729559	28.56439099283404	20.1145597533805
24-25	20.926646658814963	29.98131855776475	28.560771762141652	20.531263021278633
26-27	20.559427873298592	30.07029193693045	28.60316665444672	20.767113535324235
28-29	20.55287123893041	30.004555770095	28.814759734312705	20.627813256661884
30-31	20.85518794835467	30.32111213576563	28.47005379169325	20.35364612418645
32-33	20.54631689056219	30.15266149616616	28.857111973687292	20.443909639584355
34-35	20.819812353004018	29.856257633610923	28.736668990159203	20.58726102322585
36-37	20.966358859423963	29.97179698312143	28.404672910667113	20.657171246787495
38-39	20.73273515106314	30.05999743766901	28.65906584733083	20.54820156393702
40-41	20.608417757636964	29.784864580820454	28.8490396338691	20.75767802767349
42-43	20.600123287932917	29.766497596640274	28.819967017217873	20.81341209820894
44-45	20.823868576396833	29.864778291310117	28.723378941106485	20.587974191186564
46-47	20.814786624338247	30.083685679338313	28.56182318310313	20.53970451322031
48-49	20.710885938557986	29.825581135961926	28.62161748789659	20.841915437583502
50-51	20.639367918814127	29.794981991931973	28.60122753424164	20.96442255501226
52-53	20.69308056473028	29.830929119994547	28.66895431647673	20.807035998798444
54-55	20.82863323640867	30.100227171827992	28.486722648009728	20.58441694375361
56-57	20.790980886165155	29.868991517328574	28.343577075019695	20.99645052148658
58-59	20.737658719849676	29.695590316755055	28.620644016024727	20.94610694737054
60-61	20.94942772977974	29.957970831468778	28.296204631787784	20.796396806963696
62-63	20.73276142595654	29.928806487539276	28.462489838771166	20.87594224773302
64-65	20.79885159364259	29.74930655243607	28.555169830312664	20.896672023608673
66-67	20.65499694939007	29.77367837214787	28.480960968435394	21.090363710026658
68-69	21.040584809234698	30.038477903800665	28.19294541261947	20.727991874345168
70-71	20.969264070501914	29.706079989477324	28.32984007251495	20.99481586750581
72-73	20.80294809270663	29.879942081232674	28.52159601431042	20.795513811750283
74-75	20.792587914882677	30.033136969916857	28.344845468895958	20.829429646304508
76-77	20.90367588166918	29.889784631982884	28.280478288531054	20.92606119781688
78-79	20.901499788793558	29.874896927480982	28.484628940647273	20.738974343078187
80-81	20.85894321661011	29.863948418565517	28.42127659817854	20.855831766645828
82-83	20.736458804965473	29.789427951157922	28.688682864845088	20.78543037903152
84-85	20.70374938956123	29.793736043130465	28.460534339976274	21.04198022733203
86-87	20.95384253909904	29.782153146997697	28.350335799711896	20.91366851419137
88-89	20.836211273073946	29.622379225480927	28.516541127865636	21.024868373579494
90-91	20.744254744906545	29.695378138043065	28.424003194709467	21.136363922340923
92-93	20.87669942423572	29.788349609176173	28.305577586346914	21.02937338024119
94-95	20.78303868366379	29.679181023222277	28.486761467591894	21.051018825522043
96-97	20.81491686640119	29.799499133864167	28.326662171567524	21.058921828167115
98-99	20.764426992041525	29.761476751484416	28.34601102104908	21.128085235424987
100	20.82562279208537	29.89562169384804	28.23291325755496	21.045842256511634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15172.0
1	11208.5
2	6486.5
3	4908.5
4	3388.5
5	2440.5
6	2108.0
7	2156.5
8	2402.5
9	2412.0
10	1911.0
11	1481.0
12	1436.5
13	1458.0
14	1531.5
15	1712.0
16	2045.5
17	2508.0
18	3384.0
19	4576.5
20	6392.5
21	9373.5
22	14354.0
23	22165.0
24	29932.5
25	37357.0
26	48299.5
27	62505.0
28	81707.5
29	99563.5
30	108447.0
31	117299.0
32	128407.5
33	139115.0
34	148758.5
35	154622.5
36	156081.0
37	155496.0
38	153815.5
39	151554.5
40	150604.5
41	146159.5
42	138993.5
43	134746.0
44	131463.5
45	128308.5
46	126163.5
47	122821.5
48	118283.5
49	114508.5
50	112327.0
51	107537.0
52	100209.5
53	94867.5
54	92566.0
55	90993.0
56	83962.5
57	74779.5
58	68019.0
59	62001.5
60	54454.0
61	45430.0
62	38336.0
63	31776.5
64	25041.0
65	20012.0
66	15837.0
67	12043.0
68	9152.0
69	7048.0
70	5382.5
71	4121.5
72	3166.5
73	2411.0
74	1965.0
75	1574.0
76	1125.5
77	887.5
78	693.0
79	509.5
80	382.5
81	264.0
82	188.5
83	148.5
84	128.0
85	108.5
86	98.0
87	85.5
88	70.5
89	62.0
90	55.0
91	51.0
92	44.5
93	33.5
94	26.5
95	24.0
96	23.0
97	21.5
98	21.0
99	21.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18333180175843086
2	0.17850848378199333
3	0.19290985973059827
4	0.1820973981056933
5	0.19597300953553962
6	0.07845320992954298
7	0.0800990814665264
8	0.06736643638180745
9	0.06126299609882727
10-11	0.07539006012460162
12-13	0.07449854637540226
14-15	0.0720411687333784
16-17	0.06768646695844312
18-19	0.07236119931001407
20-21	0.07440710906779208
22-23	0.0720411687333784
24-25	0.07158398219532747
26-27	0.07572152036468856
28-29	0.07320699440540833
30-31	0.07911613040971686
32-33	0.07565294238398092
34-35	0.07518432618247868
36-37	0.08624824040331168
38-39	0.08142492242687416
40-41	0.07677304940220574
42-43	0.0803162450721006
44-45	0.0910258397259441
46-47	0.08054483834112608
48-49	0.0871054651621572
50-51	0.08285363035828337
52-53	0.08172209367660727
54-55	0.08369942545367763
56-57	0.07718451728645159
58-59	0.08486525112570754
60-61	0.08160779704209453
62-63	0.07284124517496758
64-65	0.07559579406672455
66-67	0.0760072619509704
68-69	0.07410993781805895
70-71	0.07016670392736953
72-73	0.06725213974729471
74-75	0.07270408921355229
76-77	0.07801888271839458
78-79	0.07466999132717138
80-81	0.0829679269927961
82-83	0.08409946367447221
84-85	0.08573390554800435
86-87	0.08225928785881714
88-89	0.0843280569434977
90-91	0.08241930314713497
92-93	0.07257836291558828
94-95	0.07260122224249083
96-97	0.07210974671408606
98-99	0.08189353862837638
100	0.09011146664984221
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4374582.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.46204633064802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50395832956406	45.6512382666942
2	14.902585626675135	18.02081645212963
3	4.6423358397588395	8.420553738777803
4	1.8728722568485745	4.5295075665985545
5	0.9309908190069657	2.8144805016103556
6	0.544736372806388	1.976152548636539
7	0.3435718478007259	1.454113988574369
8	0.23512523843173433	1.1372922447651348
9	0.1617883392106968	0.8803848655004174
>10	0.7864072716794525	8.307584797207896
>50	0.04750742018668234	1.9953509477586353
>100	0.025321875669839674	2.9640594634679838
>500	0.002080161455542792	0.8690533973846054
>1k	6.807798052551414E-4	0.6290824815968767
>5k	0.0	0.0
>10k+	3.782110029192205E-5	0.3503287392969455
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15320	0.35020488814702755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.5718653805094975E-5	0.0
5	0.0	0.0	0.0	6.857798070764247E-5	0.0
6	0.0	0.0	0.0	6.857798070764247E-5	0.0
7	0.0	0.0	0.0	9.143730761018995E-5	0.0
8	0.0	0.0	0.0	9.143730761018995E-5	0.0
9	0.0	0.0	0.0	1.6001528831783242E-4	0.0
10-11	0.0	0.0	0.0	1.6001528831783242E-4	0.0
12-13	0.0	0.0	0.0	1.6001528831783242E-4	0.0
14-15	0.0	0.0	0.0	1.6001528831783242E-4	0.0
16-17	0.0	0.0	0.0	1.828746152203799E-4	0.0
18-19	1.1429663451273744E-5	1.1429663451273744E-5	0.0	1.828746152203799E-4	0.0
20-21	2.2859326902547488E-5	2.2859326902547488E-5	0.0	1.9430427867165364E-4	0.0
22-23	2.2859326902547488E-5	2.2859326902547488E-5	0.0	2.9717124973311737E-4	0.0
24-25	2.2859326902547488E-5	2.2859326902547488E-5	0.0	6.172018263687822E-4	0.0
26-27	2.2859326902547488E-5	2.2859326902547488E-5	0.0	9.486620664557208E-4	0.0
28-29	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.002251643699900928	0.0
30-31	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.005760550379441967	0.0
32-33	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.012275458546668002	0.0
34-35	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.022916475219803858	0.0
36-37	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.04023241534848358	0.0
38-39	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.06437186455757374	0.0
40-41	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.08997431068842691	0.0
42-43	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.11597679504007469	0.0
44-45	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.14939713097159912	0.0
46-47	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.193149882663075	0.0
48-49	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.23732553190224803	0.0
50-51	2.2859326902547488E-5	2.2859326902547488E-5	0.0	0.2838899808027373	0.0
52-53	4.5718653805094975E-5	2.2859326902547488E-5	0.0	0.32877426917588926	0.0
54-55	4.5718653805094975E-5	2.2859326902547488E-5	0.0	0.3792248036498116	0.0
56-57	4.5718653805094975E-5	2.2859326902547488E-5	0.0	0.4423622645546478	0.0
58-59	4.5718653805094975E-5	2.2859326902547488E-5	0.0	0.5164950616996092	0.0
60-61	4.5718653805094975E-5	2.2859326902547488E-5	0.0	0.5955311844651672	0.0
62-63	4.5718653805094975E-5	2.2859326902547488E-5	0.0	0.678864860688404	0.0
64-65	4.5718653805094975E-5	2.2859326902547488E-5	0.0	0.7654674206587052	0.0
66-67	4.5718653805094975E-5	2.2859326902547488E-5	0.0	0.8670199804232724	0.0
68-69	4.5718653805094975E-5	2.2859326902547488E-5	0.0	0.9847226546444894	1.1429663451273744E-5
70-71	4.5718653805094975E-5	2.2859326902547488E-5	0.0	1.1170552980833368	2.2859326902547488E-5
72-73	4.5718653805094975E-5	2.2859326902547488E-5	0.0	1.2580059077644448	2.2859326902547488E-5
74-75	4.5718653805094975E-5	2.2859326902547488E-5	0.0	1.3998251718678494	2.2859326902547488E-5
76-77	4.5718653805094975E-5	2.2859326902547488E-5	0.0	1.554788548940219	2.2859326902547488E-5
78-79	4.5718653805094975E-5	2.2859326902547488E-5	0.0	1.7368516580555582	2.2859326902547488E-5
80-81	5.714831725636872E-5	2.2859326902547488E-5	0.0	1.944562931955556	2.2859326902547488E-5
82-83	6.857798070764247E-5	2.2859326902547488E-5	0.0	2.1679214151203476	2.2859326902547488E-5
84-85	6.857798070764247E-5	2.2859326902547488E-5	0.0	2.388651075691346	2.2859326902547488E-5
86-87	6.857798070764247E-5	2.2859326902547488E-5	0.0	2.626410934804743	2.2859326902547488E-5
88	6.857798070764247E-5	2.2859326902547488E-5	0.0	2.823424043714348	2.2859326902547488E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5405	0.0	49.79273	1
GTATCAA	9445	0.0	38.45749	1
ATCAACG	10510	0.0	33.39541	3
TCAACGC	10570	0.0	33.025528	4
TATCAAC	11440	0.0	31.009588	2
CAACGCA	11325	0.0	30.781927	5
AACGCAG	12270	0.0	28.534498	6
GTACATG	19795	0.0	26.81138	1
TACATGG	19800	0.0	25.92449	2
CGCAGAG	13440	0.0	25.455446	8
ACGCAGA	14105	0.0	24.555454	7
ACATGGG	20660	0.0	24.207705	3
CATGGGT	7960	0.0	23.227385	4
GCAGAGT	15435	0.0	21.587536	9
CAGAGTA	15050	0.0	18.907806	10-11
CATGGGG	10180	0.0	18.62422	4
TACCTGG	3035	0.0	18.602581	2
TGGTATC	2605	0.0	18.241657	2
ATGGGTG	5030	0.0	18.23822	5
GTGGTAT	2850	0.0	18.159897	1
>>END_MODULE