FastQCFastQC Report
Wed 25 May 2016
SRR2431406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4374582
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265180.6061836308017543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219620.5020365374337479No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135590.3099496134716414No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44970.10279839308075604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109150.063.9797331
GTATCAA215800.044.4329831
GTGGTAT35300.037.1681
ATCAACG274950.034.7671173
TCAACGC277300.034.4890484
TATCAAC278100.034.3921932
TGGTATC37100.034.2215542
CAACGCA286700.033.4074555
AACGCAG299300.032.017476
CGCAGAG335000.028.3529368
ACGCAGA338400.028.179177
GTACATG195800.027.8362311
TACATGG196050.027.1032582
ACATGGG197000.026.3027083
TATAACG4150.026.0609132
CATGGGT76000.025.9845854
GCAGAGT363600.025.9030199
GAGTACT257900.023.11615812-13
CAGAGTA358500.022.46319210-11
AGAGTAC344850.021.9486110-11
Produced by FastQC (version 0.11.5)