##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431406_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4374582
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65902753680238	34.0	31.0	34.0	31.0	34.0
2	32.79636271534057	34.0	31.0	34.0	31.0	34.0
3	32.886073229396544	34.0	31.0	34.0	31.0	34.0
4	36.34232573535026	37.0	37.0	37.0	35.0	37.0
5	36.247971120440766	37.0	37.0	37.0	35.0	37.0
6	36.28554591044356	37.0	37.0	37.0	35.0	37.0
7	36.243044706899994	37.0	37.0	37.0	35.0	37.0
8	36.26065096048034	37.0	37.0	37.0	35.0	37.0
9	38.06503615659736	39.0	39.0	39.0	35.0	39.0
10-11	38.0463071671762	39.0	39.0	39.0	35.0	39.0
12-13	38.02703058257909	39.0	39.0	39.0	35.0	39.0
14-15	39.510923557953646	41.0	39.0	41.0	36.0	41.0
16-17	39.538632605355204	41.0	39.5	41.0	37.0	41.0
18-19	39.60297875317002	41.0	40.0	41.0	37.0	41.0
20-21	39.59748371387255	41.0	40.0	41.0	37.0	41.0
22-23	39.545372792189056	41.0	40.0	41.0	36.5	41.0
24-25	39.49722522060394	41.0	40.0	41.0	36.0	41.0
26-27	39.38271668013081	41.0	39.0	41.0	36.0	41.0
28-29	39.30672690556492	41.0	39.0	41.0	36.0	41.0
30-31	39.142797643294834	41.0	39.0	41.0	35.5	41.0
32-33	38.93287027194827	41.0	38.5	41.0	35.0	41.0
34-35	38.8057429258384	41.0	38.0	41.0	35.0	41.0
36-37	38.66508948740702	41.0	38.0	41.0	35.0	41.0
38-39	38.5176283128308	41.0	38.0	41.0	35.0	41.0
40-41	38.37798902843746	41.0	38.0	41.0	35.0	41.0
42-43	38.21490590415267	41.0	38.0	41.0	34.0	41.0
44-45	37.98799851048626	40.0	38.0	41.0	33.5	41.0
46-47	37.652327468087236	40.0	38.0	41.0	33.0	41.0
48-49	37.344101333567416	40.0	38.0	41.0	33.0	41.0
50-51	37.184486083470375	40.0	37.5	41.0	32.0	41.0
52-53	37.124060081626084	40.0	37.0	41.0	32.0	41.0
54-55	36.99029438698372	40.0	37.0	41.0	32.0	41.0
56-57	36.84981193631757	40.0	36.5	41.0	31.5	41.0
58-59	36.6805021599778	40.0	36.0	41.0	31.0	41.0
60-61	36.533234946790344	40.0	36.0	41.0	31.0	41.0
62-63	36.380028537583705	39.5	35.0	41.0	31.0	41.0
64-65	36.15317692067494	39.0	35.0	41.0	31.0	41.0
66-67	35.89880278847214	39.0	35.0	41.0	31.0	41.0
68-69	35.612177232018965	38.5	35.0	41.0	30.0	41.0
70-71	35.315635413852114	37.0	35.0	40.0	30.0	41.0
72-73	34.999103457198885	37.0	35.0	40.0	30.0	41.0
74-75	34.66127220840757	36.5	35.0	39.0	29.0	41.0
76-77	34.32734876155024	36.0	35.0	39.0	29.0	41.0
78-79	34.00347621784207	36.0	35.0	38.5	29.0	40.0
80-81	33.73139753695324	35.0	35.0	37.0	29.0	39.0
82-83	33.51956472641272	35.0	35.0	37.0	29.0	39.0
84-85	33.24572884906489	35.0	35.0	36.5	29.0	39.0
86-87	32.99715469958044	35.0	34.0	36.0	29.0	37.0
88-89	32.75851624223755	35.0	34.0	36.0	29.0	37.0
90-91	32.57008361027408	35.0	34.0	36.0	29.0	37.0
92-93	32.40713444164494	35.0	34.0	35.0	29.0	36.0
94-95	32.26698493707513	35.0	34.0	35.0	29.0	36.0
96-97	32.124671111434196	35.0	34.0	35.0	29.0	36.0
98-99	32.01102596773818	35.0	34.0	35.0	28.5	36.0
100	31.9106646074985	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.059721646293454
1101	2	-0.0029196007105198873
1101	3	-0.01472658195590526
1101	4	0.021361839585516407
1101	5	-0.03279402235485662
1101	6	-0.03117217034940012
1101	7	-0.04949154697933267
1101	8	-0.002867503485362022
1101	9	-0.03273322309255633
1101	10-11	-0.03341567546002011
1101	12-13	0.009393267764039592
1101	14-15	0.00318323919956498
1101	16-17	-0.03929703801892259
1101	18-19	-0.04830711062768245
1101	20-21	-0.00392869010919128
1101	22-23	-0.033176389573085885
1101	24-25	-0.025328573289364442
1101	26-27	-0.006718299291996743
1101	28-29	-0.04035960993640941
1101	30-31	-0.0625386707143818
1101	32-33	-0.05244668398150054
1101	34-35	-0.08123481599434257
1101	36-37	-0.07034417006918403
1101	38-39	-0.07506801155864906
1101	40-41	-0.12597685437289385
1101	42-43	-0.14224921218757913
1101	44-45	-0.18156921396298742
1101	46-47	-0.15256947553594102
1101	48-49	-0.119389367300343
1101	50-51	-0.18067818955712056
1101	52-53	-0.10287797409407062
1101	54-55	-0.09217785415095392
1101	56-57	-0.08278665631866744
1101	58-59	-0.09280448398042296
1101	60-61	-0.10715625198328382
1101	62-63	-0.06923379419703934
1101	64-65	-0.08598780873060008
1101	66-67	-0.07756150086978408
1101	68-69	-0.044053966644455045
1101	70-71	-0.06952177342743227
1101	72-73	-0.08322683178972312
1101	74-75	-0.13703198322333776
1101	76-77	-0.15034819932733967
1101	78-79	-0.1288799200589139
1101	80-81	-0.0680180690604999
1101	82-83	-0.12490420469043784
1101	84-85	-0.09259828998384023
1101	86-87	-0.08329609448970388
1101	88-89	-0.11036282592304758
1101	90-91	-0.13577132490205202
1101	92-93	-0.14436838077512704
1101	94-95	-0.1242778719739519
1101	96-97	-0.12586700906038217
1101	98-99	-0.14527392873768719
1101	100	-0.11509133552905126
1102	1	-0.053301147070001775
1102	2	0.0012304162013947462
1102	3	0.018706039237180505
1102	4	0.024076415104708815
1102	5	-0.009275088207282067
1102	6	-0.0419799175161657
1102	7	-0.05304480562802638
1102	8	-0.027623711986123567
1102	9	0.026688187119603413
1102	10-11	0.03474267995976987
1102	12-13	0.017406041458535526
1102	14-15	0.0018588082840835796
1102	16-17	0.006401817693394207
1102	18-19	-0.025618305544405473
1102	20-21	0.03806013799952268
1102	22-23	0.025445260429911798
1102	24-25	0.005340084520859989
1102	26-27	0.0628814738239214
1102	28-29	-0.00636671671019684
1102	30-31	-0.034991990205945456
1102	32-33	0.006416570270637578
1102	34-35	-0.02025441136987638
1102	36-37	0.03826004990381904
1102	38-39	-0.030351283082559632
1102	40-41	-0.01695385712130104
1102	42-43	0.040048471794783325
1102	44-45	0.010704279841185382
1102	46-47	-0.025922346679074337
1102	48-49	0.026808994520507667
1102	50-51	-6.355469650145551E-4
1102	52-53	0.008054900963657019
1102	54-55	0.020599467167770058
1102	56-57	0.04966869562905174
1102	58-59	0.03332301427150952
1102	60-61	0.009142830556676529
1102	62-63	0.03267678640186489
1102	64-65	0.04531552737277167
1102	66-67	0.07831763888417953
1102	68-69	0.027616287813458484
1102	70-71	0.04414109242141251
1102	72-73	0.02641434094977768
1102	74-75	0.06094441735478995
1102	76-77	0.03553529235537667
1102	78-79	-0.0019875832162981055
1102	80-81	1.1924105047711464E-4
1102	82-83	0.05981463883277627
1102	84-85	0.03374903620134262
1102	86-87	0.05278829717300226
1102	88-89	0.040591903937652773
1102	90-91	0.02650460123197007
1102	92-93	0.030619771139228646
1102	94-95	0.01010205486942084
1102	96-97	0.05123860101875266
1102	98-99	0.08970154828354282
1102	100	0.1112021372370009
1103	1	-0.06599559121055876
1103	2	-0.055779469820713246
1103	3	-0.034952898215799166
1103	4	0.00717080083028776
1103	5	-0.009030004822193405
1103	6	-0.043066547992346216
1103	7	-0.07531935651838495
1103	8	-0.03175008372670618
1103	9	-0.021153371929258924
1103	10-11	0.029904566529388887
1103	12-13	0.018521688606647047
1103	14-15	0.010112683937023803
1103	16-17	0.012958288004163876
1103	18-19	0.004845256461329939
1103	20-21	-0.029551875131630823
1103	22-23	-0.009977061265118436
1103	24-25	0.01695199435724959
1103	26-27	0.017431799367834344
1103	28-29	-0.01611411773993865
1103	30-31	0.01505244435566766
1103	32-33	0.003762403458736685
1103	34-35	0.036096436194263504
1103	36-37	0.07972038356628985
1103	38-39	0.10408906405911722
1103	40-41	0.11896336356747383
1103	42-43	0.1729718868624346
1103	44-45	0.1352149844616477
1103	46-47	0.1530875067771973
1103	48-49	0.21264893494537773
1103	50-51	0.0940483468611859
1103	52-53	0.17472083829120777
1103	54-55	0.23319728235611592
1103	56-57	0.2296207195323845
1103	58-59	0.18096700298642787
1103	60-61	0.17121524171871982
1103	62-63	0.19276118026635913
1103	64-65	0.22118962122193153
1103	66-67	0.25990268699130326
1103	68-69	0.23886396908751806
1103	70-71	0.23424047929314895
1103	72-73	0.20993112851288487
1103	74-75	0.1919408379110621
1103	76-77	0.2229281780932979
1103	78-79	0.21059693849456096
1103	80-81	0.22431530368780983
1103	82-83	0.18115867174055467
1103	84-85	0.18677100618530318
1103	86-87	0.2280052607188523
1103	88-89	0.24038124366271063
1103	90-91	0.2590324049535795
1103	92-93	0.2162889826057608
1103	94-95	0.13976501878316583
1103	96-97	0.18499282662725847
1103	98-99	0.15264590980100223
1103	100	0.1627074804516866
1104	1	-0.10506919323514552
1104	2	-0.08441869582436112
1104	3	-0.05822255969535206
1104	4	-0.006670023971622641
1104	5	-0.002254598712923439
1104	6	-0.07210220906861053
1104	7	-0.045240087081900526
1104	8	-0.08993910342692146
1104	9	-0.08438415077966255
1104	10-11	-0.06256602980066361
1104	12-13	-0.03258846519870673
1104	14-15	-0.06650464032838244
1104	16-17	-0.07903308067493953
1104	18-19	-0.05934648883987137
1104	20-21	-0.035109788376956885
1104	22-23	-0.04524009532056539
1104	24-25	-0.024883971873826738
1104	26-27	-0.09893782768387638
1104	28-29	-0.08936700474502146
1104	30-31	-0.04209600547446257
1104	32-33	-0.03544609748331595
1104	34-35	-0.0315387345550775
1104	36-37	0.002276250978631822
1104	38-39	-0.04836387638321327
1104	40-41	-0.0868674738931432
1104	42-43	-0.08140665275885084
1104	44-45	-0.1111996097329282
1104	46-47	-0.1224317171974505
1104	48-49	-0.04421279190641769
1104	50-51	-0.10826099353282359
1104	52-53	0.030085393073306932
1104	54-55	0.09190033423068655
1104	56-57	0.026787377702994775
1104	58-59	0.047174886939210126
1104	60-61	3.6921557413904793E-4
1104	62-63	-0.05309082869040793
1104	64-65	-0.04728924532582113
1104	66-67	-0.09949410742672171
1104	68-69	-0.050923288891091545
1104	70-71	-0.007726556353361502
1104	72-73	0.013098880037723859
1104	74-75	-0.018620426462440776
1104	76-77	-0.0014502435039887018
1104	78-79	-0.036942261167141055
1104	80-81	-0.006782236616750481
1104	82-83	-0.008223864374159007
1104	84-85	-0.05945462405656343
1104	86-87	-0.0773689996536362
1104	88-89	-0.06567072556767783
1104	90-91	-0.001357712068468686
1104	92-93	-0.019348903197737854
1104	94-95	-0.09615625017067231
1104	96-97	-0.0753938877880671
1104	98-99	-0.02904531632017182
1104	100	0.06334701984672009
1105	1	-0.19654374469921976
1105	2	-0.1932470330619509
1105	3	-0.16035437360998372
1105	4	-0.0813023673731692
1105	5	-0.1322484043951988
1105	6	-0.05729821095569321
1105	7	-0.06493353577895533
1105	8	-0.07624472843622243
1105	9	-0.10300391939966858
1105	10-11	-0.1254911974524191
1105	12-13	-0.1122224503399778
1105	14-15	-0.14334033975848115
1105	16-17	-0.17426898956176018
1105	18-19	-0.21992474762964065
1105	20-21	-0.12410458129653534
1105	22-23	-0.16942575914536917
1105	24-25	-0.19057652468673325
1105	26-27	-0.12693026877672509
1105	28-29	-0.1400800741322712
1105	30-31	-0.21315295807702483
1105	32-33	-0.16076613577835985
1105	34-35	-0.1925084784125275
1105	36-37	-0.21644860742832606
1105	38-39	-0.18498342849747473
1105	40-41	-0.24770731679362257
1105	42-43	-0.30044313387789856
1105	44-45	-0.2308787210932266
1105	46-47	-0.21024199734207372
1105	48-49	-0.2799662661868396
1105	50-51	-0.2515375615313431
1105	52-53	-0.314791801979311
1105	54-55	-0.24644356428909475
1105	56-57	-0.14969615216925547
1105	58-59	-0.21725795935090275
1105	60-61	-0.17986793225099973
1105	62-63	-0.218263727823782
1105	64-65	-0.22161295212958265
1105	66-67	-0.25129740265618494
1105	68-69	-0.21737751271859906
1105	70-71	-0.13520048536963714
1105	72-73	-0.12887507660883557
1105	74-75	-0.1846598471094012
1105	76-77	-0.24812015900318585
1105	78-79	-0.06788894888217101
1105	80-81	-0.15069792779241453
1105	82-83	-0.1974129366814239
1105	84-85	-0.18741615213649254
1105	86-87	-0.1557759236301237
1105	88-89	-0.1388411815710171
1105	90-91	-0.11113203436197949
1105	92-93	-0.20705579848211642
1105	94-95	-0.1964405865779284
1105	96-97	-0.21332809042962353
1105	98-99	-0.18701217080046462
1105	100	-0.1817949174191078
1106	1	-0.116900525776785
1106	2	-0.08417726845497953
1106	3	-0.08913944591893141
1106	4	-0.01874964344693808
1106	5	-0.05455682349908386
1106	6	-0.08576787698108745
1106	7	-0.08097661058295103
1106	8	-0.05405689352137699
1106	9	-0.08461810830323913
1106	10-11	-0.09801562334219227
1106	12-13	-0.10868039126940232
1106	14-15	-0.11756871376077527
1106	16-17	-0.1339239365852407
1106	18-19	-0.06419694533411047
1106	20-21	-0.054933831520841636
1106	22-23	-0.06692459388434457
1106	24-25	-0.04441724028459504
1106	26-27	-0.07091786239717379
1106	28-29	-0.10502392361279789
1106	30-31	-0.17408018619629928
1106	32-33	-0.10354274976938882
1106	34-35	-0.09909796072884802
1106	36-37	-0.07999855922930976
1106	38-39	-0.1072992876291039
1106	40-41	-0.1989711848417457
1106	42-43	-0.17917976966747773
1106	44-45	-0.19819638776385062
1106	46-47	-0.21043581369993802
1106	48-49	-0.19435134656106356
1106	50-51	-0.21986919250393555
1106	52-53	-0.16856484240626202
1106	54-55	-0.2028267772890615
1106	56-57	-0.22573298122748753
1106	58-59	-0.1578143915129857
1106	60-61	-0.1912678965824881
1106	62-63	-0.18884984472716582
1106	64-65	-0.2348253144893775
1106	66-67	-0.2214135497290073
1106	68-69	-0.20480830076998302
1106	70-71	-0.1921602472034465
1106	72-73	-0.1869577858219884
1106	74-75	-0.2144275556295696
1106	76-77	-0.19695344026844452
1106	78-79	-0.18068328346826235
1106	80-81	-0.1613171739033774
1106	82-83	-0.15545611196214537
1106	84-85	-0.15729329740609188
1106	86-87	-0.14986706329140276
1106	88-89	-0.14845098313626437
1106	90-91	-0.19741300611296708
1106	92-93	-0.1776466532533263
1106	94-95	-0.20836966532534262
1106	96-97	-0.22995100270512836
1106	98-99	-0.20943271863515633
1106	100	-0.15181415025433864
1107	1	-0.24894436155724264
1107	2	-0.17341136875791108
1107	3	-0.16155712249118181
1107	4	-0.17298979017865435
1107	5	-0.22748078642359815
1107	6	-0.2296277942814413
1107	7	-0.19533208411234426
1107	8	-0.17236822037891386
1107	9	-0.19153815082900394
1107	10-11	-0.15855624778043165
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2216	42-43	0.22564881994926367
2216	44-45	0.1942123296046887
2216	46-47	0.2857851073838873
2216	48-49	0.27151123887668405
2216	50-51	0.2950283080869056
2216	52-53	0.2292994653215885
2216	54-55	0.24163208073041886
2216	56-57	0.2220341950078506
2216	58-59	0.24535111148657052
2216	60-61	0.16666228139636985
2216	62-63	0.21615155116705864
2216	64-65	0.18611823482709866
2216	66-67	0.1877115787202044
2216	68-69	0.23250961424025007
2216	70-71	0.2498042206192963
2216	72-73	0.1965568501054591
2216	74-75	0.1781556652980143
2216	76-77	0.20201783066156054
2216	78-79	0.2675658344295755
2216	80-81	0.19889741081603773
2216	82-83	0.22480880511471213
2216	84-85	0.23017844021880762
2216	86-87	0.23773795408771292
2216	88-89	0.26912221567928896
2216	90-91	0.22158000229521235
2216	92-93	0.21408165557934922
2216	94-95	0.21953619051277684
2216	96-97	0.2292034944686847
2216	98-99	0.22189970895713174
2216	100	0.24623261044735045
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50.0
3	275.0
4	83.0
5	127.0
6	274.0
7	646.0
8	1335.0
9	2234.0
10	3642.0
11	5203.0
12	7893.0
13	12049.0
14	14086.0
15	18361.0
16	22883.0
17	33651.0
18	38818.0
19	20622.0
20	12294.0
21	10984.0
22	10621.0
23	10791.0
24	11737.0
25	14299.0
26	17204.0
27	21948.0
28	27578.0
29	34883.0
30	44640.0
31	56081.0
32	71457.0
33	93636.0
34	130478.0
35	209717.0
36	347603.0
37	730825.0
38	1380080.0
39	945818.0
40	9676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2980452920906	21.216762581606186	14.643118242157371	22.842073884145847
2	14.205473774497646	22.077730880640754	40.697027146210786	23.019768198650816
3	16.11609817628783	28.344243816319846	32.53350810222517	23.00614990516715
4	10.92963853460742	18.728646531257155	40.516008158036584	29.825706776098837
5	11.153570657523519	39.14376305880717	36.083451037935035	13.619215245734276
6	26.848713774253174	40.643974670037046	18.816632080505062	13.690679475204718
7	23.691886447665173	35.3438797123931	24.087718552309685	16.876515287632053
8	24.376774740992396	35.48247581140323	21.635667133454124	18.505082314150243
9	25.45459242011458	15.931731014968758	22.623576491263844	35.99010007365282
10-11	22.334728209460927	27.604534558959003	30.26821762627835	19.792519605301717
12-13	24.02396388957848	25.966286607497587	30.180597826260886	19.829151676663052
14-15	21.292619648302356	27.18335456774551	28.709934881770977	22.814090902181157
16-17	19.1032194618823	30.523956346000602	29.383241644573125	20.98958254754397
18-19	19.03280510339453	28.20221422475331	33.21299412046212	19.551986551390037
20-21	20.630576818539463	27.276194616994264	32.93441521955698	19.158813344909294
22-23	20.146976412247167	27.36749897904473	33.06698392382628	19.418540684881826
24-25	19.883485957509066	27.40759112874836	32.97458270279661	19.734340210945966
26-27	19.583575452146885	27.492288391966852	33.00309927320923	19.921036882677036
28-29	19.58200534794503	27.429791791321563	33.17212919419075	19.816073666542657
30-31	19.887426727134283	27.755383211115692	32.793817819476324	19.5633722422737
32-33	19.62345807350956	27.645607706172072	33.10025249630274	19.63068172401563
34-35	19.893780904656783	27.482676476148484	32.786304763720466	19.837237855474275
36-37	20.078515926237962	27.608107986087184	32.35319296842755	19.960183119247308
38-39	19.938064267180884	27.727521636873593	32.4597737199238	19.874640376021723
40-41	19.90114473912052	27.575965907564786	32.422411078555584	20.100478274759105
42-43	19.9262325811053	27.643065816905015	32.19630088511759	20.2344007168721
44-45	20.228006227349933	27.818986239458543	31.823799508683532	20.12920802450799
46-47	20.34719266378852	28.19481394074029	31.221748677699818	20.236244717771378
48-49	20.39589221763168	28.142851379031264	30.826495239708457	20.6347611636286
50-51	20.38943089858347	28.22403060602853	30.60459981945625	20.781938675931745
52-53	20.394911296059572	28.399662411145187	30.557883412975773	20.647542879819465
54-55	20.48515228101489	28.63485885614592	30.4589004019619	20.421088460877293
56-57	20.400608802119194	28.372368723042584	30.3236695826624	20.90335289217582
58-59	20.325436120954322	28.138769597540144	30.70663898693366	20.829155294571876
60-61	20.487433158503045	28.3056366309691	30.448564965180243	20.758365245347612
62-63	20.342914317939766	28.22239539626287	30.66709063573186	20.767599650065506
64-65	20.311620617402877	28.114926032993807	30.745798737140866	20.82765461246245
66-67	20.261141184553402	28.22089767253616	30.533182872779342	20.9847782701311
68-69	20.683285286717258	28.355146138901393	30.226861102494397	20.734707471886956
70-71	20.550811085988624	28.101044057617585	30.27251611416627	21.07562874222752
72-73	20.447117975527895	28.23302162668466	30.458347644255525	20.861512753531915
74-75	20.47484857022518	28.39515584736953	30.196608681688016	20.933386900717274
76-77	20.588877041311594	28.34435523773721	30.09021620869729	20.9765515122539
78-79	20.562418441359444	28.289122744775362	30.290831954425347	20.85762685943985
80-81	20.477080255395588	28.308199683304352	30.249292098130386	20.965427963169674
82-83	20.310596398072978	28.28311772298649	30.46514022854396	20.941145650396574
84-85	20.296297854140242	28.34300335328449	30.170576545936385	21.190122246638886
86-87	20.4305691476193	28.29140729351474	30.09989281033739	21.17813074852857
88-89	20.232503063762607	28.160224554768597	30.28930239937017	21.31796998209862
90-91	20.15978859443186	28.173083736995746	30.192203505290856	21.474924163281543
92-93	20.204136215776323	28.274134592744822	30.130996929955906	21.390732261522952
94-95	20.078314376708175	28.134106655133284	30.280705572324273	21.506873395834265
96-97	20.07898828068477	28.26262128898066	30.11536211648543	21.543028313849145
98-99	20.034769131594647	28.127953147395058	30.09709525736244	21.740182463647855
100	20.10373591004373	28.2120071229858	29.995885309870456	21.68837165710001
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2861.0
1	1642.5
2	1829.5
3	3167.5
4	1940.5
5	1121.5
6	2821.5
7	7317.5
8	12897.5
9	14452.0
10	9596.0
11	4845.5
12	4257.0
13	4704.0
14	5261.0
15	5857.5
16	6416.5
17	7121.0
18	7977.5
19	9109.0
20	10853.0
21	13354.5
22	18093.0
23	25475.0
24	32893.5
25	40014.0
26	50016.5
27	63543.0
28	82024.0
29	99021.5
30	107603.0
31	116673.5
32	126894.0
33	136248.0
34	146254.5
35	152595.5
36	153597.0
37	153004.5
38	151359.5
39	149155.0
40	148555.0
41	144698.5
42	138165.0
43	134144.5
44	131351.5
45	128159.0
46	125839.0
47	122727.0
48	117762.5
49	113098.0
50	110889.0
51	106688.5
52	98945.0
53	92891.5
54	90219.0
55	88050.0
56	80839.5
57	72128.0
58	66344.0
59	60713.5
60	52570.5
61	43572.5
62	36921.5
63	30464.0
64	23463.5
65	18221.5
66	14049.5
67	10501.5
68	8071.5
69	6077.5
70	4397.5
71	3178.0
72	2338.0
73	1744.0
74	1395.0
75	1040.5
76	666.0
77	500.0
78	353.5
79	203.0
80	99.5
81	40.5
82	22.0
83	16.0
84	10.0
85	3.5
86	3.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.5
93	1.5
94	1.0
95	1.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005029051918560448
2	0.005326223168293565
3	9.82951056809542E-4
4	0.0
5	0.04695305745783254
6	0.0
7	0.0
8	0.0
9	9.143730761018996E-5
10-11	0.0
12-13	0.0
14-15	1.1429663451273745E-5
16-17	0.0
18-19	8.000764415891621E-5
20-21	0.0
22-23	5.714831725636872E-5
24-25	4.571865380509498E-5
26-27	1.8287461522037993E-4
28-29	1.028669710614637E-4
30-31	2.057339421229274E-4
32-33	0.001428707931409218
34-35	8.915137491993521E-4
36-37	0.0012686926430913856
38-39	0.0010858180278710057
40-41	1.028669710614637E-4
42-43	5.029051918560448E-4
44-45	1.9430427867165367E-4
46-47	1.8287461522037993E-4
48-49	1.714449517691062E-4
50-51	4.3432721114840234E-4
52-53	6.857798070764248E-4
54-55	8.686544222968047E-4
56-57	0.002057339421229274
58-59	4.91475528404771E-4
60-61	0.0012344036527375645
62-63	0.001120107018224827
64-65	0.0014629969217630394
66-67	3.0860091318439113E-4
68-69	2.514525959280224E-4
70-71	3.428899035382124E-4
72-73	2.1716360557420117E-4
74-75	6.743501436251509E-4
76-77	8.115061050404359E-4
78-79	0.0010400993740659107
80-81	8.000764415891622E-4
82-83	0.001428707931409218
84-85	0.0014858562486655868
86-87	0.002125917401936916
88-89	0.0013487002872503019
90-91	0.0011886849989324695
92-93	0.0011886849989324695
94-95	7.772171146866147E-4
96-97	2.057339421229274E-4
98-99	2.743119228305699E-4
100	2.743119228305699E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4374582.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.33184371829706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.97852229926337	41.11014712954362
2	16.183483193590146	18.232908921580144
3	5.216120669905966	8.815010831787657
4	2.1291085987772034	4.797464513823994
5	1.071343198112465	3.0175368802365976
6	0.6041074755870415	2.0418292742294506
7	0.3869470096293836	1.5258206931593379
8	0.2654235690973485	1.1961439210835567
9	0.17932557783647887	0.9091566382840058
>10	0.8902490627958521	8.830873715125415
>50	0.05855859425296018	2.2811858583061975
>100	0.03278752359520722	3.5238126072569895
>500	0.002682151978286033	1.0417922750419661
>1k	0.0012191596166949645	1.256088747021223
>5k	0.0	0.0
>10k+	1.2191596166945772E-4	1.4202279935197615
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26518	0.6061836308017543	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21962	0.5020365374337479	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13559	0.3099496134716414	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4497	0.10279839308075604	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.5718653805094975E-5	0.0
5	0.0	0.0	0.0	4.5718653805094975E-5	0.0
6	0.0	0.0	0.0	4.5718653805094975E-5	0.0
7	0.0	0.0	0.0	6.857798070764247E-5	0.0
8	0.0	0.0	0.0	6.857798070764247E-5	0.0
9	0.0	0.0	0.0	6.857798070764247E-5	0.0
10-11	0.0	0.0	0.0	6.857798070764247E-5	0.0
12-13	0.0	0.0	0.0	6.857798070764247E-5	0.0
14-15	0.0	0.0	0.0	6.857798070764247E-5	0.0
16-17	0.0	0.0	0.0	1.028669710614637E-4	0.0
18-19	0.0	0.0	0.0	1.1429663451273744E-4	0.0
20-21	0.0	0.0	0.0	1.257262979640112E-4	0.0
22-23	0.0	0.0	0.0	2.5145259592802236E-4	0.0
24-25	0.0	0.0	0.0	5.714831725636872E-4	0.0
26-27	0.0	0.0	0.0	9.029434126506257E-4	0.0
28-29	0.0	0.0	0.0	0.0022059250460958325	0.0
30-31	0.0	0.0	0.0	0.005726261389088145	0.0
32-33	0.0	0.0	0.0	0.012309747537021822	0.0
34-35	0.0	0.0	0.0	0.022939334546706408	0.0
36-37	0.0	0.0	0.0	0.04046100861750906	0.0
38-39	0.0	0.0	0.0	0.06479476210527085	0.0
40-41	0.0	0.0	0.0	0.09044292688992914	0.0
42-43	0.0	0.0	0.0	0.1167540121547613	0.0
44-45	0.0	0.0	0.0	0.1504943786629214	0.0
46-47	0.0	0.0	0.0	0.19461287958483806	0.0
48-49	0.0	0.0	0.0	0.2390056924295853	0.0
50-51	0.0	0.0	0.0	0.2858444532529051	0.0
52-53	0.0	0.0	0.0	0.3311173501834004	0.0
54-55	0.0	0.0	0.0	0.38233367210855806	0.0
56-57	0.0	0.0	0.0	0.446465513733655	0.0
58-59	0.0	0.0	0.0	0.5219012925120617	0.0
60-61	0.0	0.0	0.0	0.601954655324783	0.0
62-63	0.0	0.0	0.0	0.6864770165469524	0.0
64-65	0.0	0.0	0.0	0.7739939495933554	0.0
66-67	0.0	0.0	0.0	0.8767923426741115	0.0
68-69	0.0	0.0	0.0	0.9965637859800092	0.0
70-71	0.0	0.0	0.0	1.1306908865807066	0.0
72-73	0.0	0.0	0.0	1.2736188280388845	0.0
74-75	2.2859326902547488E-5	0.0	0.0	1.4176325875249338	0.0
76-77	2.2859326902547488E-5	0.0	0.0	1.5755333881042806	0.0
78-79	2.2859326902547488E-5	0.0	0.0	1.7604196240920846	0.0
80-81	2.2859326902547488E-5	0.0	0.0	1.9703596823650809	0.0
82-83	2.2859326902547488E-5	0.0	0.0	2.1966327297099473	0.0
84-85	2.2859326902547488E-5	0.0	0.0	2.42081414864323	0.0
86-87	2.2859326902547488E-5	0.0	0.0	2.662665827272183	0.0
88	2.2859326902547488E-5	0.0	0.0	2.8631535538709754	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10915	0.0	63.979733	1
GTATCAA	21580	0.0	44.432983	1
GTGGTAT	3530	0.0	37.168	1
ATCAACG	27495	0.0	34.767117	3
TCAACGC	27730	0.0	34.489048	4
TATCAAC	27810	0.0	34.392193	2
TGGTATC	3710	0.0	34.221554	2
CAACGCA	28670	0.0	33.407455	5
AACGCAG	29930	0.0	32.01747	6
CGCAGAG	33500	0.0	28.352936	8
ACGCAGA	33840	0.0	28.17917	7
GTACATG	19580	0.0	27.836231	1
TACATGG	19605	0.0	27.103258	2
ACATGGG	19700	0.0	26.302708	3
TATAACG	415	0.0	26.060913	2
CATGGGT	7600	0.0	25.984585	4
GCAGAGT	36360	0.0	25.903019	9
GAGTACT	25790	0.0	23.116158	12-13
CAGAGTA	35850	0.0	22.463192	10-11
AGAGTAC	34485	0.0	21.94861	10-11
>>END_MODULE