FastQCFastQC Report
Wed 25 May 2016
SRR2431405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3893213
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288080.7399543770145636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61900.052.7513541
GTATCAA110000.039.3941541
ATCAACG131100.032.189593
TCAACGC131900.031.9553954
TATCAAC137200.031.2727762
CAACGCA147900.028.4980565
GTACATG255600.028.3481481
CATGGGT98450.027.8091854
TACATGG250500.027.739492
GTACCGG2053.6379788E-1127.5417921
AACGCAG156650.026.9106456
ACATGGG260150.025.6073783
CGCAGAG167900.024.6873428
ACGCAGA172150.024.1876877
ATGGGTG55750.023.8790455
GTGGTAT29100.023.6061231
TGGTATC27850.022.805222
GCAGAGT184750.021.3925449
TCTGTCG7600.021.0243138
TACCTGG24850.020.8253362
Produced by FastQC (version 0.11.5)