##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431405_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3893213 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84605594402361 33.0 31.0 34.0 26.0 34.0 2 30.806421585461674 33.0 31.0 34.0 26.0 34.0 3 30.95656055807889 34.0 31.0 34.0 27.0 34.0 4 34.13761230120212 37.0 35.0 37.0 30.0 37.0 5 34.232307094423035 37.0 35.0 37.0 31.0 37.0 6 34.273626693427765 37.0 35.0 37.0 30.0 37.0 7 34.12458835414348 37.0 35.0 37.0 30.0 37.0 8 34.10137616410918 37.0 35.0 37.0 30.0 37.0 9 35.50503144831788 39.0 37.0 39.0 28.0 39.0 10-11 35.56125660219464 39.0 37.0 39.0 29.5 39.0 12-13 35.50593442999394 39.0 37.0 39.0 29.5 39.0 14-15 36.853694621897134 41.0 38.0 41.0 29.5 41.0 16-17 36.88951773252581 40.5 38.0 41.0 30.0 41.0 18-19 36.927781115495094 40.5 38.0 41.0 30.0 41.0 20-21 37.02590110019668 41.0 38.0 41.0 30.0 41.0 22-23 37.01470276607009 40.0 38.0 41.0 30.0 41.0 24-25 37.08145778307018 40.5 38.0 41.0 30.0 41.0 26-27 37.138514512306415 40.0 38.0 41.0 30.0 41.0 28-29 37.11698024228318 40.0 38.0 41.0 30.0 41.0 30-31 37.05481847512581 40.0 38.0 41.0 30.0 41.0 32-33 37.00088384581065 40.0 38.0 41.0 30.0 41.0 34-35 36.927601957560505 40.0 38.0 41.0 30.0 41.0 36-37 36.78909926068776 40.0 37.0 41.0 30.0 41.0 38-39 36.6628838442695 40.0 37.0 41.0 29.0 41.0 40-41 36.5967450278215 40.0 37.0 41.0 29.0 41.0 42-43 36.422731687169446 40.0 37.0 41.0 28.5 41.0 44-45 36.264068136010025 40.0 36.0 41.0 28.0 41.0 46-47 36.13310586911119 40.0 36.0 41.0 28.0 41.0 48-49 35.99048420931503 40.0 36.0 41.0 28.0 41.0 50-51 35.84855400924634 40.0 35.0 41.0 28.0 41.0 52-53 35.67679394885407 40.0 35.0 41.0 27.0 41.0 54-55 35.503330411153975 39.0 35.0 41.0 27.0 41.0 56-57 35.3057863774728 39.0 35.0 41.0 27.0 41.0 58-59 35.078954965988245 39.0 34.5 41.0 26.5 41.0 60-61 34.82934314151319 38.5 34.0 41.0 26.0 41.0 62-63 34.7055111035538 38.0 34.0 40.5 26.0 41.0 64-65 34.42689906768523 38.0 34.0 40.0 26.0 41.0 66-67 34.13376779025448 37.0 34.0 40.0 25.5 41.0 68-69 33.80889537767391 37.0 34.0 40.0 24.5 41.0 70-71 33.45086051032913 36.0 33.0 39.5 23.5 41.0 72-73 33.088988837754314 36.0 33.0 39.0 22.5 41.0 74-75 32.787061098378125 35.5 33.0 39.0 22.0 41.0 76-77 32.51611830639629 35.0 33.0 38.5 21.5 40.0 78-79 32.166416530510915 35.0 33.0 37.0 20.5 39.0 80-81 31.84535369629147 35.0 32.5 37.0 20.0 39.0 82-83 31.530792946597067 35.0 32.0 36.5 18.5 39.0 84-85 31.240729572206813 35.0 32.0 36.0 18.0 37.0 86-87 30.971677121184996 35.0 32.0 36.0 16.0 37.0 88-89 30.720574497208347 35.0 32.0 35.0 9.5 37.0 90-91 30.49717546920757 35.0 31.5 35.0 4.5 36.0 92-93 30.27759655071531 35.0 31.0 35.0 2.0 36.0 94-95 30.069969071818058 35.0 31.0 35.0 2.0 36.0 96-97 29.880855478495526 35.0 31.0 35.0 2.0 36.0 98-99 29.66482067639248 35.0 31.0 35.0 2.0 35.0 100 29.494114244455673 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3049869123192437 1101 2 0.30992659006076195 1101 3 0.34519724408923125 1101 4 0.43242023277831265 1101 5 0.37199829038890186 1101 6 0.4168189348403146 1101 7 0.43849225958656746 1101 8 0.4238854546008568 1101 9 0.38821641778830696 1101 10-11 0.40904432555212367 1101 12-13 0.3397355272252014 1101 14-15 0.4062573641781171 1101 16-17 0.3737967778205942 1101 18-19 0.3244631146351651 1101 20-21 0.37636992474443787 1101 22-23 0.37854362628667815 1101 24-25 0.3712090288596883 1101 26-27 0.39521824010168416 1101 28-29 0.38293871621598896 1101 30-31 0.4076122779623077 1101 32-33 0.35596955130151287 1101 34-35 0.4079708724278106 1101 36-37 0.4279116107381924 1101 38-39 0.43715408457147475 1101 40-41 0.37481283742145877 1101 42-43 0.4164669743660738 1101 44-45 0.41785151028491185 1101 46-47 0.39882641708671684 1101 48-49 0.43994200063441724 1101 50-51 0.4070201342023978 1101 52-53 0.353453766848169 1101 54-55 0.4460365053175437 1101 56-57 0.3384919844296448 1101 58-59 0.33864811508247783 1101 60-61 0.3688450527184699 1101 62-63 0.37015540550390114 1101 64-65 0.36642936778643076 1101 66-67 0.37151266904420766 1101 68-69 0.36119999170774975 1101 70-71 0.3625229426722498 1101 72-73 0.32805909364948604 1101 74-75 0.3753298590546166 1101 76-77 0.42601065183970377 1101 78-79 0.43006466598914983 1101 80-81 0.40190704017512857 1101 82-83 0.3678328768845951 1101 84-85 0.3665663635745773 1101 86-87 0.3200897946290553 1101 88-89 0.3172050379929452 1101 90-91 0.2538636543480379 1101 92-93 0.2834150917624392 1101 94-95 0.27031883669960166 1101 96-97 0.28389715052083275 1101 98-99 0.27988180881796865 1101 100 0.2723121053994255 1102 1 0.15791066255834707 1102 2 0.14651845107666261 1102 3 0.1822788966087323 1102 4 0.28674555663745593 1102 5 0.16931825038504655 1102 6 0.2093853730530597 1102 7 0.13784832428966354 1102 8 0.1348206859683998 1102 9 0.15454524331703112 1102 10-11 0.23387226580635456 1102 12-13 0.16804562265795653 1102 14-15 0.2147187155391137 1102 16-17 0.18098309946375934 1102 18-19 0.11321298875728303 1102 20-21 0.11572625052758667 1102 22-23 0.22954490824300677 1102 24-25 0.14110332873624287 1102 26-27 0.20349583639626445 1102 28-29 0.16559783251862825 1102 30-31 0.1872521233602953 1102 32-33 0.21815598217204268 1102 34-35 0.22229666782949664 1102 36-37 0.2111877950692289 1102 38-39 0.1732451114632525 1102 40-41 0.18162778601266893 1102 42-43 0.1935251243528313 1102 44-45 0.14843294887862157 1102 46-47 0.17231163329563515 1102 48-49 0.09391049733015677 1102 50-51 0.05946550852991095 1102 52-53 0.07338613645866587 1102 54-55 0.026794559237494298 1102 56-57 0.067616702902761 1102 58-59 0.054302634777769754 1102 60-61 0.05963442825228071 1102 62-63 0.10674841207512742 1102 64-65 0.08882734542839188 1102 66-67 0.09997962563405594 1102 68-69 0.01748690589606383 1102 70-71 0.007646553420016744 1102 72-73 -0.057653646897151134 1102 74-75 -0.050312810803262664 1102 76-77 -0.06788120720477764 1102 78-79 -0.02427476418498742 1102 80-81 -0.124588189252961 1102 82-83 -0.06536832685427996 1102 84-85 -0.015494786168684271 1102 86-87 -0.06579224833832598 1102 88-89 -0.06889232941434997 1102 90-91 -0.002840708193048158 1102 92-93 -0.03304376761611749 1102 94-95 -0.1111123242168226 1102 96-97 -0.0885533228599229 1102 98-99 -0.16041597695679144 1102 100 -0.1703521845507474 1103 1 -0.24543225386303646 1103 2 -0.24167839512959333 1103 3 -0.20505074510830212 1103 4 -0.17245107019743955 1103 5 -0.13611110395951442 1103 6 -0.11307760652805143 1103 7 -0.35811406774203647 1103 8 -0.315031428628636 1103 9 -0.3247517015195527 1103 10-11 -0.2540441842817245 1103 12-13 -0.35634271778770454 1103 14-15 -0.3380842909834101 1103 16-17 -0.4000722977459432 1103 18-19 -0.3854693310727981 1103 20-21 -0.36418137583826393 1103 22-23 -0.3819290383038165 1103 24-25 -0.3419450098695265 1103 26-27 -0.28730234956415757 1103 28-29 -0.30375149574170024 1103 30-31 -0.3279658634087994 1103 32-33 -0.268918999172044 1103 34-35 -0.292018615144201 1103 36-37 -0.40027169806943874 1103 38-39 -0.3010060751515411 1103 40-41 -0.30161767638490744 1103 42-43 -0.39583752774674963 1103 44-45 -0.5098591082359079 1103 46-47 -0.6063826211107823 1103 48-49 -0.5430757006998306 1103 50-51 -0.6389286310370039 1103 52-53 -0.702745241616725 1103 54-55 -0.6576861888896062 1103 56-57 -0.6492820717019185 1103 58-59 -0.6774812142028779 1103 60-61 -0.6995947738274566 1103 62-63 -0.6197764491888407 1103 64-65 -0.5827358610945979 1103 66-67 -0.5694196419887163 1103 68-69 -0.5313794662370697 1103 70-71 -0.5602497667088926 1103 72-73 -0.5930260267746448 1103 74-75 -0.6306759131281865 1103 76-77 -0.6203181552965482 1103 78-79 -0.6456945414943505 1103 80-81 -0.6373395333531597 1103 82-83 -0.5416723313629426 1103 84-85 -0.6193169215265542 1103 86-87 -0.635723811951415 1103 88-89 -0.6661156327678235 1103 90-91 -0.7013299298226059 1103 92-93 -0.710483252564746 1103 94-95 -0.7346346384765035 1103 96-97 -0.601989751830672 1103 98-99 -0.7676774893801124 1103 100 -0.7818483452849314 1104 1 0.021644590146433274 1104 2 0.041600517875622245 1104 3 0.05867746665255069 1104 4 0.19066163165283 1104 5 0.12493138591162989 1104 6 0.16543531106970732 1104 7 0.11113105410273505 1104 8 0.16452049404880142 1104 9 0.20981732577355672 1104 10-11 0.14851044818203718 1104 12-13 0.14721223453803134 1104 14-15 0.13545142344631955 1104 16-17 0.153982473055855 1104 18-19 0.14927443984618805 1104 20-21 0.09334275266794378 1104 22-23 0.20176284980667702 1104 24-25 0.14549239168576378 1104 26-27 0.1567137243129011 1104 28-29 0.17708756568779904 1104 30-31 0.09804547081366621 1104 32-33 0.0637821981055211 1104 34-35 0.11820894863275555 1104 36-37 0.022371963727060518 1104 38-39 0.08268387154377166 1104 40-41 0.14292296982472408 1104 42-43 0.07604024413559785 1104 44-45 0.07027002286470463 1104 46-47 -0.04969783435231534 1104 48-49 -0.07655844603910111 1104 50-51 -0.045988566198289504 1104 52-53 -0.13139086811237632 1104 54-55 -0.018467225094106254 1104 56-57 -0.06851498897187724 1104 58-59 -0.1513345630172367 1104 60-61 -0.1330621341831204 1104 62-63 -0.08117106689655174 1104 64-65 -0.05684095975166059 1104 66-67 0.037533765737990166 1104 68-69 0.022916892058290728 1104 70-71 -0.03950872105552605 1104 72-73 -0.12517638245360985 1104 74-75 -0.04687867804160817 1104 76-77 -0.06875540388003287 1104 78-79 -0.057332639331534097 1104 80-81 -0.0834651808330058 1104 82-83 -0.10195106134831988 1104 84-85 -0.08038408395542973 1104 86-87 -0.024355115550896755 1104 88-89 -0.13762422683072018 1104 90-91 -0.11737855222431648 1104 92-93 -0.1535387324220885 1104 94-95 -0.10485810674545704 1104 96-97 -0.07076992587772679 1104 98-99 -0.11605928893474982 1104 100 -0.022271932809456274 1105 1 -0.20209386577447574 1105 2 -0.178181732434453 1105 3 -0.13443896723729054 1105 4 -0.02132384180367808 1105 5 -0.10853779487358395 1105 6 -0.10100428689897001 1105 7 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-0.2931263462364342 1105 70-71 -0.19944458653621666 1105 72-73 -0.2773773837819675 1105 74-75 -0.3114306265415081 1105 76-77 -0.26027369017229773 1105 78-79 -0.2788568602841721 1105 80-81 -0.2985084538116425 1105 82-83 -0.3080249757067044 1105 84-85 -0.3115159882774954 1105 86-87 -0.34309006852476287 1105 88-89 -0.3393649720177514 1105 90-91 -0.34343334307713036 1105 92-93 -0.39313059941607165 1105 94-95 -0.3895452655456815 1105 96-97 -0.35648102796732317 1105 98-99 -0.4477674645213483 1105 100 -0.45484049016736705 1106 1 -0.15371026265714605 1106 2 -0.1392274010128105 1106 3 -0.16307548077936218 1106 4 -0.06527170232167379 1106 5 -0.06740563460257931 1106 6 -0.126629739209406 1106 7 -0.089744279247995 1106 8 -0.16579797011281983 1106 9 -0.1413237951092725 1106 10-11 -0.14358637432079746 1106 12-13 -0.07626791967231838 1106 14-15 -0.18099271171031717 1106 16-17 -0.134789085686009 1106 18-19 -0.23470868412250212 1106 20-21 -0.24573162112729108 1106 22-23 -0.10953160898011305 1106 24-25 -0.1054880819642463 1106 26-27 -0.1435610968352421 1106 28-29 -0.1859633303232613 1106 30-31 -0.14135085006586934 1106 32-33 -0.21764138986816306 1106 34-35 -0.1817562221403719 1106 36-37 -0.24565937433352047 1106 38-39 -0.2558716704251083 1106 40-41 -0.24932471063119976 1106 42-43 -0.22601708463444226 1106 44-45 -0.18809483080445233 1106 46-47 -0.23222135953722045 1106 48-49 -0.2012539479165838 1106 50-51 -0.3062927277069747 1106 52-53 -0.29921020815691435 1106 54-55 -0.33628787037594776 1106 56-57 -0.37890952098934605 1106 58-59 -0.3405817777095308 1106 60-61 -0.3690347832790053 1106 62-63 -0.36208458187311976 1106 64-65 -0.3316016547973888 1106 66-67 -0.20505660820562355 1106 68-69 -0.2061204816943487 1106 70-71 -0.28812310861756885 1106 72-73 -0.28974482291486936 1106 74-75 -0.26631276836677387 1106 76-77 -0.2880586269917984 1106 78-79 -0.2394771304744694 1106 80-81 -0.20672097162132985 1106 82-83 -0.24594724455966954 1106 84-85 -0.24869907069009756 1106 86-87 -0.2514497466290102 1106 88-89 -0.22959931878125417 1106 90-91 -0.28108107078272937 1106 92-93 -0.21039916271073977 1106 94-95 -0.21391428965454296 1106 96-97 -0.21173847378062405 1106 98-99 -0.22781525215179244 1106 100 -0.2493492179708312 1107 1 -0.14674708630062483 1107 2 -0.26127584578548735 1107 3 -0.22082961389850198 1107 4 -0.17258975466765492 1107 5 -0.11718602954029933 1107 6 -0.205273220423571 1107 7 -0.20032267839850704 1107 8 -0.1369571135422305 1107 9 -0.15399888986075894 1107 10-11 -0.17922689675206982 1107 12-13 -0.20507534119686 1107 14-15 -0.1979955109339926 1107 16-17 -0.19773690152691614 1107 18-19 -0.22671740649202832 1107 20-21 -0.1891539884039588 1107 22-23 -0.19032800855198673 1107 24-25 -0.19204586630502973 1107 26-27 -0.10991431057355783 1107 28-29 -0.0974372216543884 1107 30-31 -0.16291532367804962 1107 32-33 -0.13772906599974277 1107 34-35 -0.1624255323423398 1107 36-37 -0.12441803076044522 1107 38-39 -0.15326342012766503 1107 40-41 -0.16758694183310752 1107 42-43 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2215 9 0.050575329487266174 2215 10-11 0.047877391033807726 2215 12-13 0.12516480277355413 2215 14-15 0.10528408968568215 2215 16-17 0.13090727816442183 2215 18-19 0.15999922700014224 2215 20-21 0.029202202036998415 2215 22-23 0.029242032063230283 2215 24-25 0.10516906508764379 2215 26-27 0.08772056763478986 2215 28-29 0.04778861181687688 2215 30-31 -5.241548037346888E-4 2215 32-33 0.019353976117741922 2215 34-35 0.04272059790027072 2215 36-37 -0.017249657069115187 2215 38-39 0.03999401352819376 2215 40-41 0.1029612550166803 2215 42-43 0.16804301404180677 2215 44-45 0.18238320031094446 2215 46-47 0.22877914553264134 2215 48-49 0.24252963823284546 2215 50-51 0.29051940638276363 2215 52-53 0.3077236272399375 2215 54-55 0.3331023567164877 2215 56-57 0.31647162527333705 2215 58-59 0.3171742456978919 2215 60-61 0.3500889955850681 2215 62-63 0.2858866748915503 2215 64-65 0.24846762266945888 2215 66-67 0.31627617011653797 2215 68-69 0.2842943080266096 2215 70-71 0.2880953902849157 2215 72-73 0.3096532342769649 2215 74-75 0.30640640028155985 2215 76-77 0.31579803975115794 2215 78-79 0.3305897633955084 2215 80-81 0.35617175691927017 2215 82-83 0.3771026284133434 2215 84-85 0.3234493532219638 2215 86-87 0.3514689099508317 2215 88-89 0.43581891949726526 2215 90-91 0.4747788011948799 2215 92-93 0.44766139659202864 2215 94-95 0.4680050364831452 2215 96-97 0.44977159561891966 2215 98-99 0.5143164279260439 2215 100 0.5085510119188008 2216 1 0.10275650785833434 2216 2 0.07382405958913196 2216 3 0.07093282631965536 2216 4 0.016746786998780294 2216 5 0.010968914545905761 2216 6 0.027598112303842015 2216 7 0.06071617839081256 2216 8 -0.011989072670964163 2216 9 0.02963082370637693 2216 10-11 0.009637895825704845 2216 12-13 0.021279456290692167 2216 14-15 0.03981597824997607 2216 16-17 0.031075208508809737 2216 18-19 0.04896788468888502 2216 20-21 0.04392835244917137 2216 22-23 0.01839522215829703 2216 24-25 -0.018509953829074277 2216 26-27 -0.021396389859035025 2216 28-29 -0.04730494021468701 2216 30-31 -0.09904361211365398 2216 32-33 -0.0851720841012451 2216 34-35 -0.10530384036676566 2216 36-37 0.006676050924092181 2216 38-39 0.05200469030486943 2216 40-41 0.05610888010535575 2216 42-43 0.08923066425840886 2216 44-45 0.13596611923660618 2216 46-47 0.1823428975102246 2216 48-49 0.1677874819752816 2216 50-51 0.24093369523602348 2216 52-53 0.24374527057762663 2216 54-55 0.19730652093659273 2216 56-57 0.2384901850921537 2216 58-59 0.28687878833827085 2216 60-61 0.2394723862028485 2216 62-63 0.22201187949000456 2216 64-65 0.21741484683138168 2216 66-67 0.183549754294404 2216 68-69 0.22612990902064922 2216 70-71 0.19907939371931604 2216 72-73 0.196418131038115 2216 74-75 0.17617116995339188 2216 76-77 0.22768078884869425 2216 78-79 0.24060033478882303 2216 80-81 0.21203235448119173 2216 82-83 0.2493630143801191 2216 84-85 0.2672891996601585 2216 86-87 0.21629787685093405 2216 88-89 0.2791676718852649 2216 90-91 0.28907977991003264 2216 92-93 0.29604071115169717 2216 94-95 0.27226737343614005 2216 96-97 0.23557752565957912 2216 98-99 0.20605157419843323 2216 100 0.24565818755818114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86403.0 3 18696.0 4 13079.0 5 11669.0 6 7766.0 7 6890.0 8 8003.0 9 8332.0 10 8433.0 11 9197.0 12 10707.0 13 12662.0 14 14158.0 15 15106.0 16 15632.0 17 15114.0 18 13924.0 19 12404.0 20 11370.0 21 12116.0 22 13791.0 23 20904.0 24 34409.0 25 45462.0 26 49187.0 27 36156.0 28 34540.0 29 42568.0 30 49830.0 31 56459.0 32 72326.0 33 97426.0 34 137528.0 35 223301.0 36 381046.0 37 714478.0 38 1058254.0 39 511745.0 40 2142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81364782389188 21.65544993281282 13.781400550363623 21.749501692931684 2 15.249092858087549 22.67793027036457 39.7416671855726 22.331309685975285 3 17.667704028417177 29.296037720748185 29.986239709054903 23.05001854177974 4 11.623865248418571 19.286974972743316 39.05604004731611 30.033119731522 5 11.658026421033886 41.63873513392209 34.17411879274013 12.529119652303889 6 27.61467276746697 40.445015680427744 18.807002210683255 13.133309341422034 7 25.16830600446725 35.80737397389827 22.514277958307876 16.510042063326605 8 24.15375619285067 37.570667574844336 20.618700320646692 17.6568759116583 9 25.407298566188764 18.79601698254275 21.13948245857749 34.657201992691 10-11 22.268585762503086 29.884934706965677 28.683167065932064 19.163312464599176 12-13 23.226282736495957 28.316075351535495 28.033039908871377 20.42460200309717 14-15 21.274691435375736 29.44940645449013 25.87753035201461 23.39837175811952 16-17 20.020763179208238 32.33713511822649 26.721926333638436 20.92017536892684 18-19 20.06812976657747 31.238262684526152 28.65042399566108 20.0431835532353 20-21 22.422612804207603 30.016092624421585 27.634253114451706 19.927041456919103 22-23 21.79528817148774 30.164837784695948 27.814154352488902 20.22571969132741 24-25 21.07077407583101 30.439115458678355 27.889275153033733 20.600835312456905 26-27 20.6832993058916 30.35468868585956 28.09916091349061 20.86285109475823 28-29 20.720836969987275 30.259616928122323 28.380976445278357 20.638569656612045 30-31 21.137604887024576 30.784537720769805 27.745879452091415 20.331977940114207 32-33 20.56478266541482 30.471838946393653 28.32264959324672 20.640728794944806 34-35 20.989644081574948 30.08025756451162 28.292549072529987 20.637549281383446 36-37 21.0219869999928 30.336152298138437 27.859831910996945 20.782028790871816 38-39 20.855752374251466 30.434634514952446 28.081983220889477 20.62762988990661 40-41 20.785081818289314 30.011701179834695 28.370836917119558 20.832380084756437 42-43 20.604777387382665 30.13777879918027 28.3651142034243 20.892329610012762 44-45 20.88227067194424 30.12996224870606 28.181222664762135 20.80654441458757 46-47 20.93178541755783 30.29619428860518 28.051916068901978 20.720104224935014 48-49 20.757493686759947 30.142247459471992 28.102035533071916 20.99822332069614 50-51 20.796038227994437 30.0231248183633 27.988639520058793 21.192197433583466 52-53 20.844124269944857 30.058484102249555 28.03119974735423 21.066191880451353 54-55 20.938946437546033 30.503464701943088 27.87122954180586 20.68635931870502 56-57 20.91953597513945 30.129213831906725 27.727769374138134 21.223480818815688 58-59 20.879257185612463 29.848632686347248 28.144698005107315 21.127412122932967 60-61 21.068673875408397 30.258155078134692 27.803338032935603 20.86983301352131 62-63 20.76571003785508 30.189779205693657 28.038797407851145 21.005713348600118 64-65 20.917070281713965 29.985967511666324 27.980097978077545 21.116864228542166 66-67 20.67770399508721 30.101448414794618 27.84748189899217 21.373365691126 68-69 21.229017896905116 30.34663437446301 27.719966753287885 20.70438097534399 70-71 21.126914061827286 30.063953498832923 27.785431783148777 21.023700656191018 72-73 20.972187344415353 30.221648926750266 27.91838567489006 20.887778053944324 74-75 20.978868010759356 30.27182552662252 27.750170516696116 20.999135945922013 76-77 21.000698046049447 30.160332090491753 27.564631596589795 21.27433826686901 78-79 21.219554232149537 30.116352254203633 27.863003314527152 20.80109019911968 80-81 20.949533613623597 30.129022638730184 27.843851501857962 21.077592245788253 82-83 20.950569330534847 30.124524304662497 28.11667770147458 20.808228663328073 84-85 20.96536108107608 29.99450885909208 27.872617356426975 21.167512703404867 86-87 21.257972917529983 30.063683084304444 27.64971295954493 21.02863103862064 88-89 21.05588826254969 29.94256262763575 27.869143665667863 21.1324054441467 90-91 21.018655300145593 29.86839015372798 27.79801546032984 21.314939085796592 92-93 21.097108234108088 30.12372021218433 27.644149392696004 21.13502216101158 94-95 20.98418439856683 29.8148596069452 27.95497325595652 21.245982738531445 96-97 20.979339495139083 30.11207279012178 27.676910296014462 21.231677418724672 98-99 20.89426026762693 30.084636618319287 27.698068555558013 21.323034558495767 100 20.98720331043901 30.097954730322417 27.514245109461875 21.400596849776697 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 27601.0 1 19946.5 2 10643.5 3 7360.5 4 4628.0 5 3146.0 6 2671.5 7 2831.5 8 3404.0 9 3495.0 10 2516.0 11 1601.0 12 1465.5 13 1485.0 14 1547.0 15 1713.0 16 1930.0 17 2407.5 18 3113.5 19 4125.5 20 5519.0 21 7814.0 22 11594.0 23 16714.0 24 22474.5 25 27954.5 26 35720.0 27 46080.5 28 59365.5 29 71945.5 30 78997.5 31 86648.5 32 96514.0 33 105851.0 34 114184.5 35 121805.5 36 126399.5 37 128759.0 38 130510.5 39 130589.5 40 131595.5 41 130570.5 42 128246.0 43 127889.0 44 128050.0 45 128057.0 46 126809.5 47 123543.5 48 120657.5 49 120288.0 50 121370.0 51 116314.0 52 106316.5 53 97106.5 54 91316.0 55 89944.5 56 82091.5 57 71081.0 58 63356.0 59 55903.0 60 46971.5 61 37251.5 62 29463.0 63 23091.0 64 17757.0 65 13932.0 66 11059.0 67 8443.5 68 6287.5 69 4658.0 70 3442.0 71 2495.0 72 1862.0 73 1415.0 74 1094.5 75 865.0 76 625.5 77 479.5 78 428.0 79 327.0 80 233.0 81 191.5 82 153.5 83 116.5 84 92.5 85 85.5 86 79.0 87 63.0 88 56.5 89 51.5 90 54.0 91 51.5 92 37.0 93 31.0 94 30.0 95 22.5 96 16.5 97 20.5 98 20.0 99 23.5 100 37.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1812127926214158 2 0.17545919013421563 3 0.18992025352838388 4 0.18005693497889788 5 0.19318234065282325 6 0.07739109059792003 7 0.08029357756690939 8 0.06775894357693761 9 0.06149162658195172 10-11 0.07560593268336462 12-13 0.07416753206156457 14-15 0.07244658846048238 16-17 0.06863225824017334 18-19 0.07302451728174134 20-21 0.07504084672480031 22-23 0.07224110265736809 24-25 0.07224110265736809 26-27 0.07629944726887535 28-29 0.07311441732060384 30-31 0.07898360557205578 32-33 0.07591416138803606 34-35 0.07514358962635746 36-37 0.08653520883650599 38-39 0.08213010693224337 40-41 0.0772369762455843 42-43 0.08057612054619154 44-45 0.0910558965050204 46-47 0.08129532085709155 48-49 0.08661226601267386 50-51 0.08313185022242554 52-53 0.0817191353260148 54-55 0.08394095057218806 56-57 0.0779176479684004 58-59 0.08487847954889702 60-61 0.08135953517056478 62-63 0.07320431735946634 64-65 0.0759013185253414 66-67 0.07602974715228784 68-69 0.07457850366779316 70-71 0.07012203031275197 72-73 0.0679644293800519 74-75 0.07272913143976453 76-77 0.07881664835702543 78-79 0.07486104664707531 80-81 0.08214294979493801 82-83 0.08346576465248627 84-85 0.08504543676392738 86-87 0.0813980637586487 88-89 0.08314469308512018 90-91 0.08200167830529694 92-93 0.0724851170485663 94-95 0.07227963124545202 96-97 0.07179160246305558 98-99 0.08144943520942728 100 0.0887955526707632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3893213.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.251022484071434 #Duplication Level Percentage of deduplicated Percentage of total 1 74.45992330547122 43.37366666629239 2 15.288439892341826 17.81134511830357 3 5.0901957997120775 8.895273299320626 4 2.130217986917853 4.96349503407701 5 1.0199691295158715 2.9707122348243904 6 0.5435829168772389 1.8998556427783908 7 0.34456299833674897 1.4049802874305124 8 0.21075019251774854 0.9821131362299004 9 0.14905447094505622 0.7814317804534658 >10 0.6530845651111896 6.987067575201149 >50 0.0617964557047999 2.4839746568907186 >100 0.04546597591426311 5.08428884891049 >500 0.0020738298808869746 0.8594727231386154 >1k 8.383567155726957E-4 0.761879525266755 >5k 0.0 0.0 >10k+ 4.412403766172038E-5 0.7404434708820097 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28808 0.7399543770145636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.568572538928643E-5 0.0 4 0.0 0.0 0.0 5.137145077857286E-5 0.0 5 0.0 0.0 0.0 5.137145077857286E-5 0.0 6 0.0 0.0 0.0 5.137145077857286E-5 0.0 7 0.0 0.0 0.0 7.70571761678593E-5 0.0 8 0.0 0.0 0.0 7.70571761678593E-5 0.0 9 0.0 0.0 0.0 7.70571761678593E-5 0.0 10-11 0.0 0.0 0.0 7.70571761678593E-5 0.0 12-13 0.0 0.0 0.0 7.70571761678593E-5 0.0 14-15 0.0 0.0 0.0 7.70571761678593E-5 0.0 16-17 0.0 0.0 0.0 2.0548580311429148E-4 0.0 18-19 0.0 0.0 0.0 2.3117152850357788E-4 0.0 20-21 0.0 0.0 0.0 2.9538584197679396E-4 0.0 22-23 0.0 0.0 0.0 5.522430958696584E-4 0.0 24-25 0.0 0.0 0.0 0.001130171917128603 0.0 26-27 0.0 0.0 0.0 0.001772315051860764 0.0 28-29 0.0 0.0 0.0 0.003570315829110814 0.0 30-31 0.0 0.0 0.0 0.007962574870678795 0.0 32-33 0.0 0.0 0.0 0.01688836444345583 0.0 34-35 0.0 0.0 0.0 0.03047611317438835 0.0 36-37 0.0 0.0 0.0 0.05273279422420504 0.0 38-39 0.0 0.0 0.0 0.08220716410841122 0.0 40-41 0.0 0.0 0.0 0.11377492061184424 0.0 42-43 0.0 0.0 0.0 0.14503444841060584 0.0 44-45 0.0 0.0 0.0 0.18447487974585516 0.0 46-47 0.0 0.0 0.0 0.23293100069274403 0.0 48-49 0.0 0.0 0.0 0.2861389808366509 0.0 50-51 0.0 0.0 0.0 0.3419797478329596 0.0 52-53 0.0 0.0 0.0 0.3946483277436914 0.0 54-55 0.0 0.0 0.0 0.4522485669291662 0.0 56-57 0.0 0.0 0.0 0.5269683420866005 0.0 58-59 0.0 0.0 0.0 0.6145566656640672 0.0 60-61 0.0 0.0 0.0 0.7078600631406502 0.0 62-63 0.0 0.0 0.0 0.8037063474307724 0.0 64-65 0.0 0.0 0.0 0.9030330475111431 0.0 66-67 0.0 0.0 0.0 1.0186444974883213 0.0 68-69 0.0 0.0 0.0 1.1533789700178234 0.0 70-71 0.0 0.0 0.0 1.3025616630787988 0.0 72-73 0.0 0.0 0.0 1.4635597898188464 0.0 74-75 0.0 0.0 0.0 1.62324794456404 0.0 76-77 0.0 0.0 0.0 1.7975641199184325 0.0 78-79 0.0 0.0 0.0 2.0024206227606864 0.0 80-81 0.0 0.0 0.0 2.234683794593309 0.0 82-83 0.0 0.0 0.0 2.484015130947112 0.0 84-85 0.0 0.0 0.0 2.73407851047451 0.0 86-87 0.0 0.0 0.0 3.0052298705465126 0.0 88 0.0 0.0 0.0 3.22633259469749 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6190 0.0 52.751354 1 GTATCAA 11000 0.0 39.394154 1 ATCAACG 13110 0.0 32.18959 3 TCAACGC 13190 0.0 31.955395 4 TATCAAC 13720 0.0 31.272776 2 CAACGCA 14790 0.0 28.498056 5 GTACATG 25560 0.0 28.348148 1 CATGGGT 9845 0.0 27.809185 4 TACATGG 25050 0.0 27.73949 2 GTACCGG 205 3.6379788E-11 27.541792 1 AACGCAG 15665 0.0 26.910645 6 ACATGGG 26015 0.0 25.607378 3 CGCAGAG 16790 0.0 24.687342 8 ACGCAGA 17215 0.0 24.187687 7 ATGGGTG 5575 0.0 23.879045 5 GTGGTAT 2910 0.0 23.606123 1 TGGTATC 2785 0.0 22.80522 2 GCAGAGT 18475 0.0 21.392544 9 TCTGTCG 760 0.0 21.024313 8 TACCTGG 2485 0.0 20.825336 2 >>END_MODULE