FastQCFastQC Report
Wed 25 May 2016
SRR2431405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431405_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3893213
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355770.9138210521746434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295810.759809442740482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185420.476264720168149No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73540.18889282451281242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60520.1554500100559615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59490.152804380340865No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55120.1415797183457468No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42810.1099605903915352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122300.068.4861451
GTATCAA248550.046.3950841
TGGTATC34200.042.2127882
GTGGTAT37100.039.2964251
TATAACG2800.035.268992
ATCAACG326850.035.103983
TATCAAC328400.035.011242
TCAACGC331000.034.6767274
CAACGCA344450.033.3090255
AACGCAG355900.032.31376
CGCAGAG397300.028.840048
ACGCAGA399700.028.7139027
CATGGGT92750.028.1876684
GTACATG243350.027.0752241
GCAGAGT424650.026.7390829
TACATGG240100.026.6365832
ACATGGG242550.025.4747033
ATGGGTG48850.024.3531025
GAGTACT289500.023.71011712-13
CAGAGTA424950.022.74424710-11
Produced by FastQC (version 0.11.5)