##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431405_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3893213
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64982984491216	34.0	31.0	34.0	31.0	34.0
2	32.76773631445287	34.0	31.0	34.0	31.0	34.0
3	32.84816397150631	34.0	31.0	34.0	31.0	34.0
4	36.316889417558194	37.0	37.0	37.0	35.0	37.0
5	36.2315724826769	37.0	37.0	37.0	35.0	37.0
6	36.280121842806956	37.0	37.0	37.0	35.0	37.0
7	36.23459338083994	37.0	37.0	37.0	35.0	37.0
8	36.25412994357103	37.0	37.0	37.0	35.0	37.0
9	38.04789540156164	39.0	39.0	39.0	35.0	39.0
10-11	38.027563608772496	39.0	39.0	39.0	35.0	39.0
12-13	38.008273115290635	39.0	39.0	39.0	35.0	39.0
14-15	39.460982612561914	41.0	39.0	41.0	36.0	41.0
16-17	39.49112211430507	41.0	39.0	41.0	36.5	41.0
18-19	39.57011124230809	41.0	40.0	41.0	37.0	41.0
20-21	39.569934909803294	41.0	40.0	41.0	37.0	41.0
22-23	39.506705130184244	41.0	39.5	41.0	36.0	41.0
24-25	39.45238688456038	41.0	39.0	41.0	36.0	41.0
26-27	39.32905186538728	41.0	39.0	41.0	36.0	41.0
28-29	39.247150361411	41.0	39.0	41.0	35.0	41.0
30-31	39.04917904568797	41.0	39.0	41.0	35.0	41.0
32-33	38.801264533946636	41.0	38.0	41.0	35.0	41.0
34-35	38.66078416464755	41.0	38.0	41.0	35.0	41.0
36-37	38.51486895271335	41.0	38.0	41.0	35.0	41.0
38-39	38.36283360299064	41.0	38.0	41.0	35.0	41.0
40-41	38.21260498719181	40.0	38.0	41.0	34.5	41.0
42-43	38.01619626257285	40.0	38.0	41.0	34.0	41.0
44-45	37.731052732023656	40.0	38.0	41.0	33.0	41.0
46-47	37.30543294189144	40.0	38.0	41.0	32.5	41.0
48-49	36.91330451223706	40.0	37.0	41.0	31.0	41.0
50-51	36.71398264107307	40.0	37.0	41.0	31.0	41.0
52-53	36.63248003641208	40.0	37.0	41.0	31.0	41.0
54-55	36.474180580410064	40.0	36.0	41.0	30.5	41.0
56-57	36.3066609764223	40.0	36.0	41.0	30.0	41.0
58-59	36.10997818511343	40.0	35.0	41.0	30.0	41.0
60-61	35.94667579194871	39.0	35.0	41.0	29.5	41.0
62-63	35.77606940591229	39.0	35.0	41.0	29.5	41.0
64-65	35.52835511440037	39.0	35.0	41.0	29.0	41.0
66-67	35.254350583952125	38.5	35.0	41.0	29.0	41.0
68-69	34.95191401035597	37.5	35.0	40.5	28.5	41.0
70-71	34.640801440866454	37.0	35.0	40.0	28.0	41.0
72-73	34.3134883706594	36.5	35.0	39.0	28.0	41.0
74-75	33.97365158289567	36.0	35.0	39.0	27.5	41.0
76-77	33.64816669419321	35.5	34.5	39.0	27.0	40.5
78-79	33.330489623865944	35.0	34.0	37.0	26.5	39.5
80-81	33.070829158332714	35.0	34.0	37.0	26.5	39.0
82-83	32.88669320173338	35.0	34.0	37.0	27.0	39.0
84-85	32.635440701549086	35.0	34.0	36.0	27.0	38.0
86-87	32.40638798853287	35.0	34.0	36.0	26.5	37.0
88-89	32.18843895774518	35.0	34.0	36.0	26.0	37.0
90-91	32.01767409078311	35.0	34.0	35.0	26.0	36.5
92-93	31.864512678859338	35.0	34.0	35.0	25.5	36.0
94-95	31.73442513933864	35.0	34.0	35.0	25.0	36.0
96-97	31.600722462398025	35.0	34.0	35.0	25.0	36.0
98-99	31.492367871986453	35.0	34.0	35.0	25.0	36.0
100	31.394209101839536	35.0	34.0	35.0	24.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.01416070723220031
1101	2	0.03146318072065668
1101	3	0.058214890587038326
1101	4	0.06417067300843371
1101	5	0.008524603881411963
1101	6	0.023808261029920175
1101	7	0.060338403655812556
1101	8	0.062009351446860705
1101	9	0.03303391160805802
1101	10-11	0.07114820034065872
1101	12-13	0.04460018107031516
1101	14-15	0.06708154706163327
1101	16-17	0.06805373245213531
1101	18-19	0.042656119546521154
1101	20-21	0.0887413374251409
1101	22-23	0.06094643691426427
1101	24-25	0.05416140158146732
1101	26-27	0.09536422702210956
1101	28-29	0.07258280113232729
1101	30-31	0.07115748507985131
1101	32-33	0.0729474323374859
1101	34-35	0.128931019118113
1101	36-37	0.10281552127331395
1101	38-39	-0.0058563140504617195
1101	40-41	-0.025401587808552506
1101	42-43	-0.047944607048926
1101	44-45	-0.11115606607850026
1101	46-47	-0.0797758919331244
1101	48-49	-0.003835985976003542
1101	50-51	-0.13135089629641783
1101	52-53	-0.028403248158397787
1101	54-55	0.012219509054332889
1101	56-57	0.006698632428637552
1101	58-59	-0.0019292109271660252
1101	60-61	0.0287088759484746
1101	62-63	-3.86642822817862E-4
1101	64-65	0.05807224100997388
1101	66-67	0.04760790058529807
1101	68-69	-0.010325971051585725
1101	70-71	-0.009423007992381827
1101	72-73	-0.04384947193750577
1101	74-75	-0.06623670724351882
1101	76-77	-0.07290400863993085
1101	78-79	-0.035749531515911315
1101	80-81	0.020731843406267103
1101	82-83	-0.05186690654930004
1101	84-85	0.017876118435864896
1101	86-87	0.02733032226589671
1101	88-89	0.051955061390849266
1101	90-91	0.04363210565079356
1101	92-93	0.03135727682860434
1101	94-95	-0.020955305110607725
1101	96-97	-0.009745441651926257
1101	98-99	0.004565075677799513
1101	100	0.07058237853605576
1102	1	-0.004648245575715748
1102	2	0.018143036370027232
1102	3	0.029424421181090565
1102	4	0.04400929889617089
1102	5	0.03301417906154569
1102	6	-0.008519576657150196
1102	7	0.010060912858889992
1102	8	0.015161466808010005
1102	9	-0.027846759357018414
1102	10-11	-0.010205608950492717
1102	12-13	-0.024192214467021245
1102	14-15	-0.017630071373602618
1102	16-17	0.038758419478419626
1102	18-19	0.03726321804669652
1102	20-21	0.022278881535676476
1102	22-23	0.029236533082297456
1102	24-25	0.045787725164075255
1102	26-27	0.030300747272598016
1102	28-29	-0.01086251245412484
1102	30-31	-4.930043919557647E-4
1102	32-33	-0.015077238661689307
1102	34-35	0.03121201957686992
1102	36-37	0.016177638238247027
1102	38-39	0.05669393719912108
1102	40-41	0.010588030796121473
1102	42-43	0.013295648404579197
1102	44-45	0.020485024511486927
1102	46-47	-0.070932404628266
1102	48-49	-0.08661454640191835
1102	50-51	-0.05661380124356441
1102	52-53	-0.01485892129470301
1102	54-55	-0.05124552140412675
1102	56-57	-0.04273141383581702
1102	58-59	-0.019548850860481082
1102	60-61	-0.13456059755962713
1102	62-63	-0.054316257239548804
1102	64-65	-0.09478546663257958
1102	66-67	-0.09847964168702106
1102	68-69	-0.1002015131256968
1102	70-71	-0.12994155559873377
1102	72-73	-0.07368086484220981
1102	74-75	-0.06319698950834862
1102	76-77	-0.10300047771873011
1102	78-79	-0.07664878711808143
1102	80-81	-0.08831330159706141
1102	82-83	-0.07991143295275549
1102	84-85	-0.04420245706875647
1102	86-87	-0.07727506322945743
1102	88-89	-0.06307487756437524
1102	90-91	-0.06479836899806557
1102	92-93	-0.0640676815717498
1102	94-95	-0.07037962359843064
1102	96-97	-0.04917312487550518
1102	98-99	-0.0372506241845727
1102	100	-0.029493201474590336
1103	1	-0.08385943129860607
1103	2	-0.07659074253328413
1103	3	-0.03032156200649183
1103	4	0.0027348380884077983
1103	5	-0.05850657049153796
1103	6	-0.015629585318514216
1103	7	-0.09088022097994042
1103	8	-0.00738087633713036
1103	9	0.03324933237465899
1103	10-11	0.052680819439444804
1103	12-13	0.0233792424485344
1103	14-15	0.0031928836560695117
1103	16-17	0.01768401520251217
1103	18-19	-0.026386186733247996
1103	20-21	0.008911949090979476
1103	22-23	0.03922552379112432
1103	24-25	0.012214277216443747
1103	26-27	-0.012822147553457341
1103	28-29	-0.012591931168088877
1103	30-31	-0.07228914110611129
1103	32-33	-0.11169221375442362
1103	34-35	-0.0999650099052829
1103	36-37	-0.13040331960679907
1103	38-39	-0.1966088167726241
1103	40-41	-0.23246779086978364
1103	42-43	-0.21401413639919298
1103	44-45	-0.220357856861213
1103	46-47	-0.28460514849131613
1103	48-49	-0.3394329943715846
1103	50-51	-0.3289923502228618
1103	52-53	-0.34437342831064655
1103	54-55	-0.4256074518346935
1103	56-57	-0.4452449115832877
1103	58-59	-0.4806339506584294
1103	60-61	-0.44676134024449965
1103	62-63	-0.4447962746183549
1103	64-65	-0.47302454883515566
1103	66-67	-0.43701010459797374
1103	68-69	-0.42292710746119155
1103	70-71	-0.4317515829393699
1103	72-73	-0.49228347596221056
1103	74-75	-0.45075897059594894
1103	76-77	-0.47883533728300165
1103	78-79	-0.4272645690648247
1103	80-81	-0.4858159562842417
1103	82-83	-0.5157572270376818
1103	84-85	-0.48515612898880534
1103	86-87	-0.4411556357526507
1103	88-89	-0.4069875326481096
1103	90-91	-0.3727628444568438
1103	92-93	-0.38998086992949865
1103	94-95	-0.42763968400499763
1103	96-97	-0.38310965712302547
1103	98-99	-0.40026809792109574
1103	100	-0.3948343359543074
1104	1	-0.1326904131890032
1104	2	-0.08208104514820036
1104	3	-0.10056547529593018
1104	4	-0.016402247416195337
1104	5	-0.02642909105070146
1104	6	-0.08642925715756178
1104	7	-0.0601500251382987
1104	8	-0.11854900861507645
1104	9	-0.054755212813226706
1104	10-11	-0.0722143376369786
1104	12-13	-0.06295067767963047
1104	14-15	-0.08222588837834621
1104	16-17	-0.07913959542387516
1104	18-19	-0.08254807591427493
1104	20-21	-0.09042444463243982
1104	22-23	-0.08666832391945434
1104	24-25	-0.08299208903839173
1104	26-27	-0.09760184603509003
1104	28-29	-0.1577721940064336
1104	30-31	-0.060810076716038
1104	32-33	-0.06069496048293388
1104	34-35	-0.07740716386282287
1104	36-37	-0.05195243949208361
1104	38-39	-0.1073279618319134
1104	40-41	-0.17311588338547068
1104	42-43	-0.17292527109490408
1104	44-45	-0.05141270479929716
1104	46-47	-0.013677814783441988
1104	48-49	-0.015441840850968447
1104	50-51	-0.0697669208969458
1104	52-53	-0.05349904548229745
1104	54-55	-0.02867103750691058
1104	56-57	-0.05940781498301817
1104	58-59	-0.0429239640057375
1104	60-61	-0.048270541771977094
1104	62-63	-0.07544161403171046
1104	64-65	0.008440415742633434
1104	66-67	-0.05827961191818787
1104	68-69	-0.07031479512970407
1104	70-71	-0.024794702128211554
1104	72-73	-0.03796147927945981
1104	74-75	-0.02266981807927948
1104	76-77	-0.04671482151305639
1104	78-79	-0.07160215365296807
1104	80-81	-0.12526998741010686
1104	82-83	-0.08252646242968353
1104	84-85	-0.08628355138390731
1104	86-87	-0.07709492493583525
1104	88-89	-0.036856143392220986
1104	90-91	-0.0747831584887173
1104	92-93	-0.08335398417147388
1104	94-95	-0.132879787958192
1104	96-97	-0.13373536515421947
1104	98-99	-0.1239881329745316
1104	100	-0.04738264337196796
1105	1	-0.15549956426380618
1105	2	-0.16511775211689184
1105	3	-0.14307188829991446
1105	4	-0.07763752451185013
1105	5	-0.11636796033727848
1105	6	-0.07033361864019128
1105	7	-0.031341869274179146
1105	8	-0.07880140638041411
1105	9	-0.054043072765992406
1105	10-11	-0.11375048102410545
1105	12-13	-0.13156137330194184
1105	14-15	-0.16248965824303951
1105	16-17	-0.19590656599925182
1105	18-19	-0.24131332739631262
1105	20-21	-0.10945714490546976
1105	22-23	-0.18047038578198027
1105	24-25	-0.1813074268398509
1105	26-27	-0.151009389926557
1105	28-29	-0.09277947186846092
1105	30-31	-0.14796556213017453
1105	32-33	-0.1928650272181045
1105	34-35	-0.14373339279634934
1105	36-37	-0.11459150309815413
1105	38-39	-0.067248318740873
1105	40-41	-0.10691323988270796
1105	42-43	-0.11208649125698145
1105	44-45	-0.014652414396415736
1105	46-47	-0.02537401994121069
1105	48-49	-0.06594169552410278
1105	50-51	-0.18574153688141593
1105	52-53	-0.20008267703114768
1105	54-55	-0.2328828033902539
1105	56-57	-0.17735712617462696
1105	58-59	-0.15516281239237628
1105	60-61	-0.1344350240134915
1105	62-63	-0.1832438174455575
1105	64-65	-0.17913121581286617
1105	66-67	-0.11466675655047709
1105	68-69	-0.13321457999985853
1105	70-71	-0.13787740303476426
1105	72-73	-0.05008131449632458
1105	74-75	-0.09877046272648471
1105	76-77	-0.1586198372545624
1105	78-79	-0.13188538298362573
1105	80-81	-0.10777349003718939
1105	82-83	-0.18684731625286588
1105	84-85	-0.18429476503794717
1105	86-87	-0.21862240054479543
1105	88-89	-0.16535360308368752
1105	90-91	-0.17305429350115276
1105	92-93	-0.22841064373199416
1105	94-95	-0.2698202523207236
1105	96-97	-0.2631323348042578
1105	98-99	-0.30130674923749723
1105	100	-0.26723701318002213
1106	1	-0.11987366987972337
1106	2	-0.0972503069472026
1106	3	-0.10596022714283748
1106	4	-0.03918756885492769
1106	5	-0.04202908115837545
1106	6	-0.1024726844634003
1106	7	-0.09854701512369957
1106	8	-0.05045993097819235
1106	9	-0.0902614456648152
1106	10-11	-0.09840753061837404
1106	12-13	-0.11399816496042803
1106	14-15	-0.12170880144816465
1106	16-17	-0.1006753238905489
1106	18-19	-0.08835122964238451
1106	20-21	-0.0672290038950294
1106	22-23	-0.11067095088126422
1106	24-25	-0.0743900600126608
1106	26-27	-0.11331638788993104
1106	28-29	-0.09376541776395442
1106	30-31	-0.1288390447404737
1106	32-33	-0.09194560451449263
1106	34-35	-0.06287746834033925
1106	36-37	-0.08753647135635845
1106	38-39	-0.12655118763659345
1106	40-41	-0.20924193221232912
1106	42-43	-0.27717251326588865
1106	44-45	-0.19976923929809942
1106	46-47	-0.25343049979396426
1106	48-49	-0.20505040018500154
1106	50-51	-0.23168916447620092
1106	52-53	-0.21085175632278208
1106	54-55	-0.245068390210875
1106	56-57	-0.24428798045963163
1106	58-59	-0.23009962971110554
1106	60-61	-0.3007314293058627
1106	62-63	-0.3703847125551434
1106	64-65	-0.3386230407730366
1106	66-67	-0.33074102841666075
1106	68-69	-0.3555943288714616
1106	70-71	-0.28731192782572634
1106	72-73	-0.24050665766417723
1106	74-75	-0.28020670477232557
1106	76-77	-0.27974639314241756
1106	78-79	-0.22197613848748432
1106	80-81	-0.2016552220162069
1106	82-83	-0.2509862274594923
1106	84-85	-0.18566483455582272
1106	86-87	-0.17432170785563983
1106	88-89	-0.23300480030303916
1106	90-91	-0.2534485052627886
1106	92-93	-0.20187330924013835
1106	94-95	-0.22922759660215775
1106	96-97	-0.2944251899364154
1106	98-99	-0.2666945356443584
1106	100	-0.27213591917410795
1107	1	-0.2494268573164078
1107	2	-0.1627927999951453
1107	3	-0.12706360779247206
1107	4	-0.16016419192131082
1107	5	-0.22115804165419917
1107	6	-0.17069848699875934
1107	7	-0.1501264779520568
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2216	26-27	-0.02891171555429395
2216	28-29	-0.03526086780324533
2216	30-31	-0.024033926230004
2216	32-33	0.020455581124807054
2216	34-35	0.058379222625390526
2216	36-37	0.037448731856315476
2216	38-39	0.0729468269695488
2216	40-41	0.07454899574977247
2216	42-43	0.1374192451255425
2216	44-45	0.11623616315589658
2216	46-47	0.09496262110644693
2216	48-49	0.08148947698146713
2216	50-51	0.16358655209828044
2216	52-53	0.16367587725502375
2216	54-55	0.17665701605314155
2216	56-57	0.14621322947856186
2216	58-59	0.20632595844879376
2216	60-61	0.17446066528405169
2216	62-63	0.11142451499958383
2216	64-65	0.12936339934704932
2216	66-67	0.15914523744118014
2216	68-69	0.22052646457787262
2216	70-71	0.14427283119550083
2216	72-73	0.14476866803808264
2216	74-75	0.12766696145482115
2216	76-77	0.16189043363883115
2216	78-79	0.07830554590284322
2216	80-81	0.09643673135177266
2216	82-83	0.09249192919290294
2216	84-85	0.15879980280351447
2216	86-87	0.09828028500252373
2216	88-89	0.12717460569405148
2216	90-91	0.11189205885193587
2216	92-93	0.09471963947331474
2216	94-95	0.09061796823408841
2216	96-97	0.1463380882179841
2216	98-99	0.1078069390033427
2216	100	0.10217190924672437
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47.0
3	480.0
4	91.0
5	148.0
6	347.0
7	776.0
8	1490.0
9	2505.0
10	3594.0
11	5268.0
12	8058.0
13	12131.0
14	13924.0
15	19006.0
16	25425.0
17	39347.0
18	47476.0
19	23940.0
20	13549.0
21	11475.0
22	10992.0
23	10778.0
24	11820.0
25	13539.0
26	16578.0
27	20793.0
28	25916.0
29	33163.0
30	41661.0
31	51608.0
32	65052.0
33	84229.0
34	117459.0
35	197367.0
36	320597.0
37	694304.0
38	1216161.0
39	724790.0
40	7329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72269198541579	20.706718609763527	14.495612356401704	22.074977048418972
2	14.924960994512773	21.834647558387502	40.25564070462122	22.984750742478504
3	16.816830855369503	28.691664378478997	31.52322919318203	22.96827557296947
4	10.995339838842622	18.46528818228029	39.632483503985014	30.906888474892074
5	10.962654104625658	39.509578796108045	35.413510224303565	14.114256874962738
6	27.217699108679643	39.879220582074495	18.419028190854185	14.484052118391674
7	24.17471122181088	35.57015760504242	23.078547205097692	17.176583968049012
8	25.303264938239955	34.40402567236881	20.898060291075776	19.394649098315455
9	26.34426955025042	15.3814165222486	22.078433457626623	36.19588046987435
10-11	22.88160190567534	27.468186302675964	29.210371998655095	20.439839792993602
12-13	24.818857843123403	26.2369924275913	29.22606597686795	19.71808375241735
14-15	22.118954190279343	26.807459545624656	28.582933941708298	22.490652322387707
16-17	19.05320875071567	30.1106181449615	29.240822426104096	21.595350678218736
18-19	18.88735288172154	27.41768940856275	33.863255806068565	19.831701903647144
20-21	20.969957329583217	26.381079892248366	33.56661112127838	19.082351656890037
22-23	20.32912669538674	26.536700287577453	33.82078859306917	19.313384423966635
24-25	19.77725844075001	26.737219388158078	33.84203503970951	19.643487131382408
26-27	19.41552049306388	26.748522908167622	33.96556695218531	19.870389646583185
28-29	19.456343013737897	26.69145513270096	34.226435599326365	19.625766254234776
30-31	19.87169443384193	27.15364920325305	33.60803409837548	19.366622264529543
32-33	19.372833862851362	26.933729720538285	34.0886831607102	19.60475325590015
34-35	19.83727955039325	26.677471886735265	33.82509927617012	19.66014928670136
36-37	19.884197107393543	26.926985151163564	33.25219544910352	19.936622292339372
38-39	19.78318213499494	27.071474173203853	33.36673365996472	19.77861003183648
40-41	19.752761700988195	26.816423219157983	33.41169203028608	20.019123049567742
42-43	19.69598993730982	26.982553012070937	33.172561291905986	20.148895758713252
44-45	20.095399560952938	27.025205072068527	32.73681102546758	20.142584341510958
46-47	20.254731064696326	27.426471872524534	31.941621862096103	20.377175200683038
48-49	20.39228604710001	27.57083174402581	31.282773447583672	20.754108761290503
50-51	20.530039896511802	27.6088870395093	30.899536948742007	20.961536115236896
52-53	20.54659616740535	27.78721021477274	30.811212413486643	20.854981204335267
54-55	20.65338171200069	28.196541846554073	30.61757290191864	20.532503539526598
56-57	20.589658241931698	27.694872967552115	30.624605794915876	21.090862995600315
58-59	20.489192478715726	27.301675245104178	31.11213512101832	21.096997155161773
60-61	20.558445162825684	27.69037748578182	30.76493132261361	20.986246028778886
62-63	20.333671918134485	27.53206869833815	31.042592461070562	21.091666922456803
64-65	20.355272175068247	27.453112590402235	31.04162792346364	21.149987311065882
66-67	20.296162806064373	27.45300680302163	30.89046494313863	21.36036544777537
68-69	20.864303984226865	27.656938941262233	30.59362190813629	20.885135166374617
70-71	20.783983990566128	27.463712733302348	30.533292047720582	21.21901122841094
72-73	20.617409446336275	27.538986220213307	30.627343427422538	21.216260906027877
74-75	20.696953510960753	27.662032116879065	30.35176968788934	21.28924468427084
76-77	20.79265052963793	27.5302558051217	30.288028149749618	21.38906551549075
78-79	20.95957938695016	27.57260174184332	30.425337985749163	21.04248088545736
80-81	20.706997605942597	27.53190417657127	30.448233661852136	21.312864555633997
82-83	20.577110190292913	27.55886227009655	30.785486119744494	21.078541419866042
84-85	20.519366500169784	27.557404436389305	30.469199104276573	21.45402995916434
86-87	20.730211093400445	27.591546092229795	30.256776384170646	21.42146643019911
88-89	20.55944727733021	27.454867043874852	30.41581346174988	21.569872217045056
90-91	20.33752177851936	27.442745258299727	30.378141622857047	21.841591340323866
92-93	20.376005126934	27.612443859944285	30.317813398610127	21.693737614511583
94-95	20.167449468293803	27.29126320949652	30.609236755249476	21.9320505669602
96-97	20.186812664629787	27.557346710486602	30.315190183923015	21.940650440960596
98-99	20.05424335173467	27.43917850526919	30.332084487911732	22.174493655084408
100	20.153780951402446	27.45856878660228	30.147564985102232	22.240085276893044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5965.0
1	3343.5
2	2649.5
3	4430.5
4	2601.0
5	1502.0
6	4044.0
7	10431.5
8	18250.5
9	19916.5
10	12246.0
11	5204.5
12	4277.0
13	4716.5
14	5317.0
15	5925.5
16	6329.0
17	6874.0
18	7748.5
19	8673.5
20	10000.5
21	12027.5
22	15424.0
23	20222.0
24	25447.0
25	30545.0
26	37886.5
27	47174.5
28	59405.5
29	71501.0
30	78194.5
31	85660.0
32	95291.0
33	104412.0
34	112599.5
35	119586.0
36	123492.5
37	126136.0
38	129203.0
39	130200.5
40	130519.0
41	129141.0
42	127467.5
43	127608.5
44	128226.0
45	127717.5
46	125892.0
47	123705.5
48	120543.0
49	118813.5
50	120770.5
51	116581.0
52	104797.5
53	94504.0
54	88554.0
55	85430.0
56	77276.0
57	66985.0
58	60069.5
59	54086.5
60	45219.0
61	34901.5
62	27494.5
63	21488.5
64	16227.5
65	12543.5
66	9738.0
67	7289.5
68	5360.5
69	3868.5
70	2644.0
71	1729.0
72	1197.5
73	869.0
74	664.5
75	489.5
76	297.0
77	226.5
78	188.0
79	98.5
80	35.0
81	19.5
82	15.5
83	11.0
84	9.0
85	6.5
86	6.5
87	7.0
88	3.5
89	2.0
90	1.0
91	0.5
92	1.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006036145466482312
2	0.00439225904156798
3	7.705717616785929E-4
4	0.0
5	0.04369141888717622
6	0.0
7	0.0
8	0.0
9	5.137145077857287E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.137145077857287E-5
20-21	1.2842862694643217E-5
22-23	2.5685725389286434E-5
24-25	3.8528588083929645E-5
26-27	1.4127148964107538E-4
28-29	1.1558576425178894E-4
30-31	1.7980007772500501E-4
32-33	0.0012842862694643215
34-35	8.476289378464523E-4
36-37	0.0011815433679071758
38-39	9.632147020982413E-4
40-41	1.4127148964107538E-4
42-43	5.008716450910854E-4
44-45	2.1832866580893468E-4
46-47	2.3117152850357788E-4
48-49	2.0548580311429148E-4
50-51	3.9812874353393975E-4
52-53	7.192003109000201E-4
54-55	9.50371839403598E-4
56-57	0.0024016153238982816
58-59	5.522430958696582E-4
60-61	0.001194386230601819
62-63	0.0012200719559911056
64-65	0.0014640863471893266
66-67	4.2381446892322615E-4
68-69	2.8254297928215075E-4
70-71	2.1832866580893468E-4
72-73	2.6970011658750755E-4
74-75	6.806717228160905E-4
76-77	8.861575259303819E-4
78-79	9.889004274875276E-4
80-81	8.091003497625226E-4
82-83	0.0014640863471893266
84-85	0.0012072290932964623
86-87	0.002016329443058985
88-89	0.0012586005440750352
90-91	0.0010788004663500302
92-93	0.0012329148186857486
94-95	9.889004274875276E-4
96-97	2.0548580311429148E-4
98-99	4.3665733161786936E-4
100	3.339144300607236E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3893213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.27320911385664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.35947661740111	38.015483200941716
2	16.94222826515975	18.051337384490953
3	5.719980308934418	9.141651213750166
4	2.40339804097007	5.121469056817276
5	1.1897258954032022	3.169025820699255
6	0.6764322374957159	2.1621429623677924
7	0.3795396224051202	1.4153505569967508
8	0.2499364422299413	1.0651933081671208
9	0.17454455374842345	0.8368693660372095
>10	0.7694668774529173	7.450408933267479
>50	0.07321969691719758	2.744692140018657
>100	0.05736754080144653	5.8784468716916365
>500	0.003235271913881772	1.2168144086715957
>1k	0.001110615694543533	0.9382965724245714
>5k	1.93150555572788E-4	0.6396880292387057
>10k+	1.4486291667959098E-4	2.153130174419096
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35577	0.9138210521746434	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29581	0.759809442740482	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18542	0.476264720168149	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7354	0.18889282451281242	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6052	0.1554500100559615	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5949	0.152804380340865	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5512	0.1415797183457468	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4281	0.1099605903915352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.568572538928643E-5	0.0
4	0.0	0.0	0.0	5.137145077857286E-5	0.0
5	0.0	0.0	0.0	5.137145077857286E-5	0.0
6	0.0	0.0	0.0	5.137145077857286E-5	0.0
7	0.0	0.0	0.0	5.137145077857286E-5	0.0
8	0.0	0.0	0.0	5.137145077857286E-5	0.0
9	0.0	0.0	0.0	1.2842862694643217E-4	0.0
10-11	0.0	0.0	0.0	1.2842862694643217E-4	0.0
12-13	0.0	0.0	0.0	1.2842862694643217E-4	0.0
14-15	0.0	0.0	0.0	1.2842862694643217E-4	0.0
16-17	0.0	0.0	0.0	2.0548580311429148E-4	0.0
18-19	0.0	0.0	0.0	2.5685725389286434E-4	0.0
20-21	0.0	0.0	0.0	3.210715673660804E-4	0.0
22-23	0.0	0.0	0.0	5.650859585643015E-4	0.0
24-25	0.0	0.0	0.0	0.001130171917128603	0.0
26-27	0.0	0.0	0.0	0.0017594721891661205	0.0
28-29	0.0	0.0	0.0	0.003596001554500101	0.0
30-31	0.0	0.0	0.0	0.00806531777223594	0.0
32-33	0.0	0.0	0.0	0.01691405016884512	0.0
34-35	0.0	0.0	0.0	0.03063022752672407	0.0
36-37	0.0	0.0	0.0	0.053015337203487195	0.0
38-39	0.0	0.0	0.0	0.08275940720428088	0.0
40-41	0.0	0.0	0.0	0.11458402096160678	0.0
42-43	0.0	0.0	0.0	0.1463572632681541	0.0
44-45	0.0	0.0	0.0	0.18623435193502128	0.0
46-47	0.0	0.0	0.0	0.23510144448813872	0.0
48-49	0.0	0.0	0.0	0.2885919676113277	0.0
50-51	0.0	2.568572538928643E-5	0.0	0.3452418349573989	0.0
52-53	0.0	2.568572538928643E-5	0.0	0.39882225811945043	0.0
54-55	0.0	2.568572538928643E-5	0.0	0.45709032616504675	0.0
56-57	0.0	2.568572538928643E-5	0.0	0.5327861588872739	0.0
58-59	0.0	2.568572538928643E-5	0.0	0.6212863257160602	0.0
60-61	0.0	2.568572538928643E-5	0.0	0.7158354808740236	0.0
62-63	0.0	2.568572538928643E-5	0.0	0.8125550798273817	0.0
64-65	0.0	2.568572538928643E-5	0.0	0.9134229234311095	0.0
66-67	0.0	2.568572538928643E-5	0.0	1.0308580599109272	0.0
68-69	0.0	2.568572538928643E-5	0.0	1.1669667187487558	0.0
70-71	0.0	2.568572538928643E-5	0.0	1.3184225985066833	0.0
72-73	0.0	2.568572538928643E-5	0.0	1.4816810690809878	0.0
74-75	0.0	2.568572538928643E-5	0.0	1.6433855532692405	0.0
76-77	0.0	2.568572538928643E-5	0.0	1.8200648153594474	0.0
78-79	0.0	2.568572538928643E-5	0.0	2.0283760482665603	0.0
80-81	0.0	2.568572538928643E-5	0.0	2.264132478752126	0.0
82-83	0.0	2.568572538928643E-5	0.0	2.517779016971329	0.0
84-85	0.0	2.568572538928643E-5	0.0	2.771040269309694	0.0
86-87	0.0	2.568572538928643E-5	0.0	3.046673788462126	0.0
88	0.0	2.568572538928643E-5	0.0	3.2715394713826345	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12230	0.0	68.486145	1
GTATCAA	24855	0.0	46.395084	1
TGGTATC	3420	0.0	42.212788	2
GTGGTAT	3710	0.0	39.296425	1
TATAACG	280	0.0	35.26899	2
ATCAACG	32685	0.0	35.10398	3
TATCAAC	32840	0.0	35.01124	2
TCAACGC	33100	0.0	34.676727	4
CAACGCA	34445	0.0	33.309025	5
AACGCAG	35590	0.0	32.3137	6
CGCAGAG	39730	0.0	28.84004	8
ACGCAGA	39970	0.0	28.713902	7
CATGGGT	9275	0.0	28.187668	4
GTACATG	24335	0.0	27.075224	1
GCAGAGT	42465	0.0	26.739082	9
TACATGG	24010	0.0	26.636583	2
ACATGGG	24255	0.0	25.474703	3
ATGGGTG	4885	0.0	24.353102	5
GAGTACT	28950	0.0	23.710117	12-13
CAGAGTA	42495	0.0	22.744247	10-11
>>END_MODULE