FastQCFastQC Report
Wed 25 May 2016
SRR2431404_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431404_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183772
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248430.7803008506890569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.046.6382521
GTATCAA73250.038.3444061
ATCAACG82650.032.670043
TCAACGC82950.032.6622244
CAACGCA90100.030.1751565
TATCAAC93050.030.0301532
GTACATG170100.029.5670991
TACATGG167200.028.7261142
AACGCAG96750.028.2715536
CATGGGT80700.027.3945374
ACATGGG171800.026.8065013
CGCAGAG105450.025.3588588
ACGCAGA110200.024.4790367
GCAGAGT117600.022.0598539
ATGGGTG43200.021.99455
ATGGGTA36000.021.8202845
CATGGGG66350.019.9916114
TGGTATC20250.018.8168722
AGAGTAC109200.018.41995410-11
GAGTACA120150.018.403881
Produced by FastQC (version 0.11.5)