##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431404_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3183772
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.721402474800332	33.0	31.0	34.0	26.0	34.0
2	30.70615860683491	33.0	31.0	34.0	26.0	34.0
3	30.874819239568662	33.0	31.0	34.0	27.0	34.0
4	34.095329376601086	37.0	35.0	37.0	30.0	37.0
5	34.18136380368946	37.0	35.0	37.0	30.0	37.0
6	34.22236956666495	37.0	35.0	37.0	30.0	37.0
7	34.06434003439945	37.0	35.0	37.0	30.0	37.0
8	34.05816151407827	37.0	35.0	37.0	30.0	37.0
9	35.47452078854893	39.0	37.0	39.0	29.0	39.0
10-11	35.522690695187976	39.0	37.0	39.0	29.5	39.0
12-13	35.47141535260691	39.0	37.0	39.0	29.0	39.0
14-15	36.80173423222517	40.0	38.0	41.0	29.0	41.0
16-17	36.81765748301072	40.0	38.0	41.0	29.5	41.0
18-19	36.82449936741701	40.0	38.0	41.0	29.5	41.0
20-21	36.92003368959838	40.0	38.0	41.0	30.0	41.0
22-23	36.8836837248396	40.0	38.0	41.0	29.0	41.0
24-25	36.932765757095666	40.0	38.0	41.0	30.0	41.0
26-27	36.982717826527775	40.0	38.0	41.0	30.0	41.0
28-29	36.95819879689877	40.0	38.0	41.0	30.0	41.0
30-31	36.89082745246833	40.0	38.0	41.0	30.0	41.0
32-33	36.84529058613494	40.0	38.0	41.0	30.0	41.0
34-35	36.77522542443366	40.0	37.5	41.0	30.0	41.0
36-37	36.637600619642356	40.0	37.0	41.0	29.5	41.0
38-39	36.507084521127766	40.0	37.0	41.0	29.0	41.0
40-41	36.447016777583315	40.0	37.0	41.0	29.0	41.0
42-43	36.26290450446828	40.0	36.5	41.0	28.0	41.0
44-45	36.09887988210211	40.0	36.0	41.0	28.0	41.0
46-47	35.95878960553708	40.0	36.0	41.0	27.0	41.0
48-49	35.81922888950591	40.0	35.0	41.0	27.0	41.0
50-51	35.67881494026582	40.0	35.0	41.0	27.0	41.0
52-53	35.51496369714917	40.0	35.0	41.0	27.0	41.0
54-55	35.34903912717368	39.0	35.0	41.0	27.0	41.0
56-57	35.16062535256921	39.0	35.0	41.0	26.5	41.0
58-59	34.94270035668383	39.0	34.5	41.0	26.0	41.0
60-61	34.7041796648755	38.5	34.0	40.5	26.0	41.0
62-63	34.604444979100265	38.0	34.0	40.0	26.0	41.0
64-65	34.35732316887014	38.0	34.0	40.0	25.5	41.0
66-67	34.09748546692414	37.5	34.0	40.0	25.0	41.0
68-69	33.807205258416744	37.0	33.5	40.0	24.5	41.0
70-71	33.487964590429215	36.5	33.0	39.5	23.5	41.0
72-73	33.15381252175093	36.0	33.0	39.0	23.0	41.0
74-75	32.872035120605375	36.0	33.0	39.0	22.5	41.0
76-77	32.60828303659935	35.0	33.0	38.5	22.0	40.0
78-79	32.26256277145474	35.0	33.0	37.0	20.5	39.5
80-81	31.93330411222914	35.0	32.0	37.0	20.0	39.0
82-83	31.604250555630237	35.0	32.0	36.5	19.5	39.0
84-85	31.29690898091949	35.0	32.0	36.0	18.0	37.5
86-87	31.011535373764204	35.0	32.0	36.0	16.5	37.0
88-89	30.74426717742351	35.0	32.0	35.5	11.5	37.0
90-91	30.508363664232235	35.0	31.0	35.0	7.0	36.0
92-93	30.277846058071997	35.0	31.0	35.0	2.0	36.0
94-95	30.060919249242723	35.0	31.0	35.0	2.0	36.0
96-97	29.857071737549045	35.0	31.0	35.0	2.0	36.0
98-99	29.638612469737154	35.0	31.0	35.0	2.0	35.0
100	29.46520950620836	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10972675697297518
1101	2	0.1409368684366683
1101	3	0.11222203047975299
1101	4	0.18675534589029752
1101	5	0.12312455469222527
1101	6	0.1521098476647751
1101	7	0.12881914700003705
1101	8	-0.0014752848822041642
1101	9	0.09480673026470754
1101	10-11	0.04184033330253811
1101	12-13	-0.05458983342491308
1101	14-15	0.09846229640785964
1101	16-17	0.07999900091694201
1101	18-19	0.007043484761922514
1101	20-21	0.03780222699040792
1101	22-23	0.12800699383080882
1101	24-25	0.05733061841230125
1101	26-27	0.08010178415606362
1101	28-29	0.11425284909209665
1101	30-31	0.09819904935196888
1101	32-33	0.13808868836322574
1101	34-35	0.1773010187668902
1101	36-37	0.08169311104614252
1101	38-39	0.13688144821530557
1101	40-41	0.08475169648281877
1101	42-43	0.04501845189092535
1101	44-45	0.05148250080961958
1101	46-47	0.019527844871539912
1101	48-49	0.004753109354780349
1101	50-51	-0.07218853013763038
1101	52-53	-0.08859703528670337
1101	54-55	-0.11039781731825116
1101	56-57	-0.16412983937322423
1101	58-59	-0.09345785209431767
1101	60-61	-0.07313211224997218
1101	62-63	-0.09044587924130809
1101	64-65	-0.013112020707531258
1101	66-67	-0.004505272839821828
1101	68-69	-0.014072622924565792
1101	70-71	-0.08262132627199037
1101	72-73	-0.08509711740279613
1101	74-75	-0.006333232927566712
1101	76-77	0.008243621462639794
1101	78-79	-0.0124754332372774
1101	80-81	-0.11805687291165867
1101	82-83	-0.0023941509202316524
1101	84-85	0.035075935104071476
1101	86-87	-0.0023728213119618147
1101	88-89	-0.0033116469629739242
1101	90-91	-0.02293402819298862
1101	92-93	-0.05530024140615453
1101	94-95	0.05632077728747831
1101	96-97	-0.05030599735911423
1101	98-99	-0.02408444205634197
1101	100	-0.03210081565773848
1102	1	-0.019800055399905148
1102	2	0.0018921687397437381
1102	3	0.030850959281380597
1102	4	0.06865816259210789
1102	5	0.03966277688651587
1102	6	0.09047088119842073
1102	7	0.01004418881300495
1102	8	0.037525554972312136
1102	9	0.016606138864247555
1102	10-11	0.09761903569599184
1102	12-13	0.03728315348138267
1102	14-15	0.03330264403016514
1102	16-17	-0.020060445289345807
1102	18-19	-0.04889152195621449
1102	20-21	0.0044524181900982285
1102	22-23	-0.050290919567920866
1102	24-25	-0.015710793767937048
1102	26-27	0.03281454208197232
1102	28-29	-0.07870705475487938
1102	30-31	-0.04908086342005902
1102	32-33	-0.015939772058885637
1102	34-35	-0.03117886774953149
1102	36-37	-0.10935804307539598
1102	38-39	-0.1473370336403832
1102	40-41	-0.1732844309459054
1102	42-43	-0.17378888511154145
1102	44-45	-0.24238630607509748
1102	46-47	-0.22923133444145805
1102	48-49	-0.24664331983708365
1102	50-51	-0.27472866676416885
1102	52-53	-0.367937240381913
1102	54-55	-0.4210941450827832
1102	56-57	-0.3621674883279482
1102	58-59	-0.36807631669367424
1102	60-61	-0.3829161506536565
1102	62-63	-0.38219802543954984
1102	64-65	-0.3307868645713654
1102	66-67	-0.26939074901599724
1102	68-69	-0.3112474089193782
1102	70-71	-0.32506930388329636
1102	72-73	-0.35373807229025545
1102	74-75	-0.3744574952135977
1102	76-77	-0.32871937089656456
1102	78-79	-0.23941759970938392
1102	80-81	-0.33640174487037555
1102	82-83	-0.30706915551844105
1102	84-85	-0.33975788572835697
1102	86-87	-0.3985388541254338
1102	88-89	-0.3062654484701639
1102	90-91	-0.3271570050446897
1102	92-93	-0.30400347680593853
1102	94-95	-0.4009446369189824
1102	96-97	-0.36860522353789804
1102	98-99	-0.39242213547395366
1102	100	-0.3793149966511393
1103	1	-0.2944062810109287
1103	2	-0.2619609092973647
1103	3	-0.16574370026424745
1103	4	-0.1760953651132624
1103	5	-0.12419046281038959
1103	6	-0.12104456158429144
1103	7	-0.17005877000000424
1103	8	-0.1720212410344999
1103	9	-0.188935789797128
1103	10-11	-0.19209683797934218
1103	12-13	-0.17491005953241512
1103	14-15	-0.29020289778302555
1103	16-17	-0.2824278467305703
1103	18-19	-0.28321263511784167
1103	20-21	-0.3043541838111139
1103	22-23	-0.39798684026778375
1103	24-25	-0.29012293524981203
1103	26-27	-0.2887117526917322
1103	28-29	-0.3466029440003666
1103	30-31	-0.3221743459669213
1103	32-33	-0.33075358270123445
1103	34-35	-0.37228936891568054
1103	36-37	-0.4653833460662611
1103	38-39	-0.41879421885239765
1103	40-41	-0.4410596296084748
1103	42-43	-0.5174140274587629
1103	44-45	-0.5865271200690998
1103	46-47	-0.5352048254685329
1103	48-49	-0.5767258517804805
1103	50-51	-0.6992444206265063
1103	52-53	-0.6662378033505902
1103	54-55	-0.5878921564825674
1103	56-57	-0.6330371094736051
1103	58-59	-0.6032315482008812
1103	60-61	-0.5770102565144981
1103	62-63	-0.5251026269931884
1103	64-65	-0.6469320590838876
1103	66-67	-0.5816288110753334
1103	68-69	-0.6893483035677761
1103	70-71	-0.7067873364097039
1103	72-73	-0.7200723279311916
1103	74-75	-0.622906450325523
1103	76-77	-0.6099321703799951
1103	78-79	-0.5742750076885876
1103	80-81	-0.6044389016810676
1103	82-83	-0.617505981065122
1103	84-85	-0.6983239495040721
1103	86-87	-0.7427786270072794
1103	88-89	-0.7869692736411018
1103	90-91	-0.8078652181243058
1103	92-93	-0.8350991306747702
1103	94-95	-0.9085560418788425
1103	96-97	-0.967632539655078
1103	98-99	-1.0062767954676168
1103	100	-0.94858508651782
1104	1	-0.2341284792573468
1104	2	-0.2625004788393568
1104	3	-0.17798800969680784
1104	4	-0.11092188120114344
1104	5	-0.1090162543074058
1104	6	-0.09743848526564136
1104	7	-0.14582120591586545
1104	8	-0.12001284380122001
1104	9	-0.17645993029338314
1104	10-11	-0.15945401998262554
1104	12-13	-0.17804273011272187
1104	14-15	-0.2306158954299775
1104	16-17	-0.2156645466831435
1104	18-19	-0.21601041074240612
1104	20-21	-0.2651348607320898
1104	22-23	-0.21402953698896
1104	24-25	-0.2605007041097025
1104	26-27	-0.25295886770361875
1104	28-29	-0.18190341997908632
1104	30-31	-0.2267281397286709
1104	32-33	-0.328888103038949
1104	34-35	-0.2749627126946663
1104	36-37	-0.3799153711135901
1104	38-39	-0.34771408903851864
1104	40-41	-0.377708218264992
1104	42-43	-0.44322859290157623
1104	44-45	-0.46495241826102784
1104	46-47	-0.5053965248655956
1104	48-49	-0.5683912568691483
1104	50-51	-0.5559582880832536
1104	52-53	-0.6230271695286476
1104	54-55	-0.4622405146098245
1104	56-57	-0.4708300412766633
1104	58-59	-0.6232935777352111
1104	60-61	-0.6695791306980894
1104	62-63	-0.5207945697889755
1104	64-65	-0.48820781202265096
1104	66-67	-0.4734331781069443
1104	68-69	-0.4108140980032644
1104	70-71	-0.5535376238991034
1104	72-73	-0.5668739747849401
1104	74-75	-0.5430525734135543
1104	76-77	-0.5350564832832987
1104	78-79	-0.5264526948656716
1104	80-81	-0.5253922372097435
1104	82-83	-0.5624196449255123
1104	84-85	-0.5191761749868995
1104	86-87	-0.5346013503405374
1104	88-89	-0.5270173600422261
1104	90-91	-0.5394156083142008
1104	92-93	-0.6114467352275348
1104	94-95	-0.6085410442153076
1104	96-97	-0.5946124841650899
1104	98-99	-0.6829374232695393
1104	100	-0.6968469571161009
1105	1	-0.20254926622616054
1105	2	-0.24856831937652757
1105	3	-0.12823447068673488
1105	4	-0.1289509437202625
1105	5	-0.1467793935704904
1105	6	-0.20140218548152689
1105	7	-0.2094870267013178
1105	8	-0.13380250927983894
1105	9	-0.27539618366644447
1105	10-11	-0.2701276681100353
1105	12-13	-0.10750309333390362
1105	14-15	-0.19113440825733363
1105	16-17	-0.2576067798899615
1105	18-19	-0.16140602362511203
1105	20-21	-0.1745067213417144
1105	22-23	-0.23843446826353443
1105	24-25	-0.27091741637593714
1105	26-27	-0.1814798873530563
1105	28-29	-0.26102843097881845
1105	30-31	-0.26840642404750525
1105	32-33	-0.12822043608535694
1105	34-35	-0.15855269898576552
1105	36-37	-0.21912455630261007
1105	38-39	-0.25169619286616296
1105	40-41	-0.3217027445305618
1105	42-43	-0.3494976135033454
1105	44-45	-0.375373485750913
1105	46-47	-0.33610996410307337
1105	48-49	-0.45179363352586677
1105	50-51	-0.4185616799761078
1105	52-53	-0.38534331767056784
1105	54-55	-0.4636136607921273
1105	56-57	-0.500981254641971
1105	58-59	-0.3614573305711275
1105	60-61	-0.41811890237715943
1105	62-63	-0.3186871105639142
1105	64-65	-0.27619166061988665
1105	66-67	-0.396644152734531
1105	68-69	-0.2960601860073666
1105	70-71	-0.2556619679912586
1105	72-73	-0.27966767878427845
1105	74-75	-0.3414096490439107
1105	76-77	-0.3313081097918271
1105	78-79	-0.30501494396570195
1105	80-81	-0.3351544598657519
1105	82-83	-0.25945320158875873
1105	84-85	-0.3297911504346054
1105	86-87	-0.3848230200573468
1105	88-89	-0.3494259237966695
1105	90-91	-0.3517802446128222
1105	92-93	-0.42627272059130306
1105	94-95	-0.41116286652128764
1105	96-97	-0.338002259708027
1105	98-99	-0.3083640676791468
1105	100	-0.3945538313564505
1106	1	-0.1773057026885425
1106	2	-0.20706335515419738
1106	3	-0.10644979171281577
1106	4	-0.012767770040674975
1106	5	0.011171397690674212
1106	6	0.011296824280123019
1106	7	-0.052363287302789274
1106	8	-0.17055239222166563
1106	9	-0.11214615347890344
1106	10-11	-0.13139476750828294
1106	12-13	-0.09491002230934953
1106	14-15	-0.15512572727496377
1106	16-17	-0.04160596938694994
1106	18-19	-0.16033953457993277
1106	20-21	-0.2302382936914853
1106	22-23	-0.11960026347296804
1106	24-25	-0.08705758909212591
1106	26-27	-0.16751506387851833
1106	28-29	-0.2189943160124841
1106	30-31	-0.14215670311941864
1106	32-33	-0.25039414347205025
1106	34-35	-0.1763780968315416
1106	36-37	-0.20235496241413387
1106	38-39	-0.3025394461018749
1106	40-41	-0.22142321655083919
1106	42-43	-0.2935845466833271
1106	44-45	-0.31524345778725404
1106	46-47	-0.3509701414755355
1106	48-49	-0.329617884679287
1106	50-51	-0.3950251460969909
1106	52-53	-0.4202354721230819
1106	54-55	-0.41497926714479405
1106	56-57	-0.34802498184385655
1106	58-59	-0.38444712240303147
1106	60-61	-0.402488377947833
1106	62-63	-0.4451404996913624
1106	64-65	-0.3499240226237177
1106	66-67	-0.23488499635261917
1106	68-69	-0.28984847966832916
1106	70-71	-0.2737501495022343
1106	72-73	-0.15857820376957932
1106	74-75	-0.24723625786631942
1106	76-77	-0.22817403452536666
1106	78-79	-0.2704050539178624
1106	80-81	-0.2582256929162057
1106	82-83	-0.23556204006758463
1106	84-85	-0.2863994646491612
1106	86-87	-0.2647198524934531
1106	88-89	-0.3237872074666406
1106	90-91	-0.3279320406197286
1106	92-93	-0.393616029858574
1106	94-95	-0.3391182271777531
1106	96-97	-0.33135350721375545
1106	98-99	-0.3748783053415643
1106	100	-0.29687414596713424
1107	1	-0.405913071202459
1107	2	-0.48953420954122606
1107	3	-0.5101149681007726
1107	4	-0.4502660588735239
1107	5	-0.41958506156394293
1107	6	-0.5024408481336948
1107	7	-0.5984056830111655
1107	8	-0.47091647789054747
1107	9	-0.5571669866221072
1107	10-11	-0.4839983357945883
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2216	58-59	0.9650956032299405
2216	60-61	0.9691388372821734
2216	62-63	0.9296649188874682
2216	64-65	0.8604411707415665
2216	66-67	0.8134344109767682
2216	68-69	0.8975493430105175
2216	70-71	0.8404551973126644
2216	72-73	0.8762472749978514
2216	74-75	0.8667948566983128
2216	76-77	0.8489802683810694
2216	78-79	0.8460968192494107
2216	80-81	0.8311538460125192
2216	82-83	0.9248687362271966
2216	84-85	0.9294517568228606
2216	86-87	0.9282580379517498
2216	88-89	0.8498044452793714
2216	90-91	0.9560694572719051
2216	92-93	0.9889091497318923
2216	94-95	0.9617773370240812
2216	96-97	0.9878944203415898
2216	98-99	0.96367578773161
2216	100	1.0074469422760224
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78067.0
3	16562.0
4	12005.0
5	10592.0
6	7001.0
7	5737.0
8	6390.0
9	6705.0
10	6822.0
11	7557.0
12	8271.0
13	9555.0
14	10121.0
15	10376.0
16	10339.0
17	10014.0
18	9935.0
19	9478.0
20	9344.0
21	9639.0
22	11108.0
23	16589.0
24	26984.0
25	35793.0
26	40440.0
27	32502.0
28	28919.0
29	35228.0
30	42429.0
31	47784.0
32	61328.0
33	82035.0
34	117102.0
35	189193.0
36	312512.0
37	567779.0
38	856439.0
39	423480.0
40	1618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.944677363278394	22.084344760427467	13.937667249269648	22.033310627024495
2	14.79719145329424	22.875365627362598	40.17469570773848	22.152747211604684
3	17.108696685081657	29.325962303026376	30.675258580244915	22.89008243164706
4	11.474934281835358	19.55936186914888	39.61023740342851	29.355466445587247
5	11.539738365845393	41.40629695441934	34.78620959618924	12.267755083546023
6	27.310156541724133	41.04200638940681	18.823950594622186	12.823886474246867
7	24.677906604309417	35.869113892300156	23.22162232162474	16.23135718176569
8	23.498604416399026	38.28273917185211	20.964428253865787	17.254228157883077
9	24.826280368133826	19.093147537943963	21.494345528512774	34.58622656540944
10-11	21.92669043989162	29.948495885941323	29.32962302973937	18.795190644427684
12-13	22.718832922510448	28.350849291517143	28.637652644209048	20.29266514176336
14-15	20.753638283859292	29.725376691065158	26.244721547745687	23.276263477329863
16-17	19.86273837476629	32.61745605563128	26.976227659314343	20.543577910288086
18-19	19.92400843708129	31.52460233921253	28.821178444395063	19.73021077931111
20-21	21.727240782345554	30.480492278756806	28.095789178567344	19.696477760330296
22-23	21.28542254832553	30.492185333051513	28.179097347232712	20.04329477139025
24-25	20.760959652612616	30.653523140038967	28.218797350970064	20.36671985637835
26-27	20.451147717820284	30.6706082936664	28.26577788855375	20.612466099959565
28-29	20.497375036675	30.58517973380906	28.54357476835624	20.373870461159704
30-31	20.7756361113809	30.881360524460476	28.142037084010298	20.20096628014832
32-33	20.360345381148683	30.72006685972549	28.521619638504248	20.39796812062158
34-35	20.71524424386654	30.414939474340535	28.4465963421685	20.423219939624424
36-37	20.864651520773933	30.601474227888115	28.00710787884732	20.526766372490634
38-39	20.641643473323214	30.711192856874558	28.278308177022847	20.368855492779378
40-41	20.510471216530657	30.404418501658476	28.479527623650373	20.60558265816049
42-43	20.435130961425244	30.498064865738172	28.466040303221853	20.60076386961473
44-45	20.624035715767675	30.50662094686267	28.37923860682855	20.490104730541105
46-47	20.65517830472716	30.60437195210212	28.23113519280152	20.5093145503692
48-49	20.636108148004016	30.437156571871167	28.251695912756347	20.675039367368473
50-51	20.629043165817006	30.32473573111471	28.24307063793084	20.803150465137442
52-53	20.6009896315631	30.432097834088665	28.262917483134707	20.70399505121353
54-55	20.73951774886604	30.671998818104377	28.12564241999415	20.46284101303543
56-57	20.69461753524635	30.396742452935456	28.050987966500447	20.85765204531775
58-59	20.684016613349836	30.072530166155502	28.35719205091508	20.886261169579576
60-61	20.7789885052919	30.51778285831753	27.985486357430588	20.71774227895998
62-63	20.587479588198235	30.434678069879194	28.149483456284763	20.828358885637808
64-65	20.67981625568308	30.272551974717082	28.211997441534827	20.835634328065012
66-67	20.527426210069986	30.242355808783838	28.13367247273959	21.096545508406585
68-69	20.938630314718594	30.502797931630145	27.92703187919674	20.63153987445453
70-71	20.83282260938764	30.22396737904014	28.08147256956689	20.86173744200533
72-73	20.70513254667148	30.332544848635614	28.205993290363455	20.756329314329452
74-75	20.72690334078902	30.381792560773942	28.100849596055678	20.790454502381362
76-77	20.73217284901713	30.37584434969927	27.953367521432	20.938615279851604
78-79	20.83820440671387	30.306580997588494	28.168065193152238	20.687149402545398
80-81	20.720419276707762	30.324275586391895	28.12469794980706	20.83060718709329
82-83	20.67025698669614	30.325065098518483	28.296339394945264	20.708338519840115
84-85	20.671271127222113	30.203475789332728	28.09409344653366	21.031159636911504
86-87	20.897283791435946	30.22119339542651	28.026405972336406	20.855116840801138
88-89	20.724805781220777	30.177724131535978	28.107021109062707	20.99044897818054
90-91	20.698721938278357	30.086063280714658	28.10371599746022	21.11149878354676
92-93	20.757405131064445	30.197923800609676	27.98783283920219	21.056838229123677
94-95	20.66624917398523	30.028027535659085	28.233212354745156	21.072510935610527
96-97	20.718975242550467	30.22831440587433	28.01980005085317	21.032910300722033
98-99	20.61271443189878	30.204087790895866	27.98564657294798	21.19755120425737
100	20.621295767138882	30.25065086361633	27.876043880876082	21.2520094883687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22916.0
1	16734.0
2	9026.0
3	6150.0
4	3934.0
5	2665.5
6	2194.5
7	2307.5
8	2729.0
9	2785.0
10	2068.0
11	1443.0
12	1338.5
13	1340.0
14	1431.5
15	1602.0
16	1742.5
17	2039.5
18	2680.5
19	3487.0
20	4678.0
21	6645.0
22	10033.0
23	15161.0
24	20650.5
25	26518.5
26	34310.0
27	43434.5
28	54731.5
29	65488.0
30	72902.0
31	79996.0
32	87047.5
33	93561.5
34	98944.5
35	103058.5
36	105355.5
37	107215.5
38	108485.5
39	108151.0
40	108904.5
41	108222.0
42	105705.5
43	104659.5
44	103287.0
45	100970.5
46	99272.0
47	96699.0
48	93319.5
49	90827.5
50	89800.0
51	85590.5
52	77736.0
53	70675.0
54	66202.0
55	64029.5
56	58970.5
57	52369.5
58	47391.0
59	43140.5
60	37743.5
61	31192.0
62	25929.0
63	20915.0
64	16321.0
65	12982.5
66	10162.0
67	7871.5
68	6032.0
69	4567.5
70	3508.5
71	2763.5
72	2197.5
73	1747.0
74	1430.5
75	1143.0
76	858.0
77	686.0
78	544.0
79	372.5
80	256.0
81	186.0
82	159.0
83	135.5
84	111.5
85	96.0
86	77.5
87	65.0
88	58.5
89	59.0
90	48.5
91	36.0
92	29.0
93	20.5
94	19.0
95	22.5
96	21.5
97	18.0
98	21.5
99	21.0
100	29.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1729709288227926
2	0.16744289478015387
3	0.18044633849408814
4	0.1704581860761386
5	0.18289626267207576
6	0.07085934545564193
7	0.07283813036863192
8	0.06121669516535731
9	0.055814298260051284
10-11	0.06851935377282041
12-13	0.06751425667415883
14-15	0.06553547176116883
16-17	0.06171924371468811
18-19	0.06605372495266622
20-21	0.06784405415965716
22-23	0.06578674603583423
24-25	0.06529990212867001
26-27	0.06833089806682137
28-29	0.06622647601649867
30-31	0.07202148897596938
32-33	0.06864499091015311
34-35	0.06870780947881946
36-37	0.07894723617143438
38-39	0.07423584352145819
40-41	0.0703882061906443
42-43	0.07346631605529541
44-45	0.08342305918891177
46-47	0.07470698278645581
48-49	0.07916710116176662
50-51	0.07539798704178566
52-53	0.07494255241895462
54-55	0.0767014723416124
56-57	0.0702625690533116
58-59	0.07773797872460717
60-61	0.0738589321094601
62-63	0.0658181553201674
64-65	0.06837801199332114
66-67	0.06877062804748581
68-69	0.06709023133566097
70-71	0.06325829864701367
72-73	0.06063562340519359
74-75	0.0654412439081693
76-77	0.07084364081347533
78-79	0.06751425667415883
80-81	0.07502107562978756
82-83	0.07663865377294606
84-85	0.0788058943919351
86-87	0.07445570851179041
88-89	0.07654442591994652
90-91	0.07513100812495367
92-93	0.06573963210933446
94-95	0.0653156067708366
96-97	0.06479735357933922
98-99	0.07448711779612359
100	0.08210386924691844
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3183772.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.996719715354054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.74005299472029	48.973683291651646
2	14.052037511798574	17.70464537120832
3	4.094693879942596	7.738568480247578
4	1.6437463575435545	4.142025142772218
5	0.7397039763727438	2.3299462035943304
6	0.43963223327828277	1.6617233146600277
7	0.24204411882859478	1.0673589858816384
8	0.17538886635600498	0.883913860401835
9	0.12109778152726573	0.6865886700921905
>10	0.662081357578613	7.579648793403482
>50	0.05955550738376545	2.583651414588364
>100	0.028621356074371385	3.1026192488263766
>500	9.458190121139405E-4	0.3862578474600525
>1k	3.484596352633833E-4	0.3802994972463174
>5k	0.0	0.0
>10k+	4.9779947894769044E-5	0.779069877965718
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24843	0.7803008506890569	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.281856866634922E-5	0.0
4	0.0	0.0	0.0	6.281856866634922E-5	0.0
5	0.0	0.0	0.0	6.281856866634922E-5	0.0
6	0.0	0.0	0.0	6.281856866634922E-5	0.0
7	0.0	0.0	0.0	6.281856866634922E-5	0.0
8	0.0	0.0	0.0	6.281856866634922E-5	0.0
9	0.0	0.0	0.0	1.5704642166587305E-4	0.0
10-11	0.0	0.0	0.0	1.7275106383246035E-4	0.0
12-13	0.0	0.0	0.0	1.8845570599904766E-4	0.0
14-15	0.0	0.0	0.0	1.8845570599904766E-4	0.0
16-17	0.0	0.0	0.0	1.8845570599904766E-4	0.0
18-19	0.0	0.0	0.0	1.8845570599904766E-4	0.0
20-21	0.0	0.0	0.0	2.0416034816563496E-4	0.0
22-23	0.0	0.0	0.0	3.140928433317461E-4	0.0
24-25	0.0	0.0	0.0	6.910042553298414E-4	0.0
26-27	0.0	0.0	0.0	0.0012720760154935717	0.0
28-29	0.0	0.0	0.0	0.002575561315320318	0.0
30-31	0.0	0.0	0.0	0.0066587682786330175	0.0
32-33	0.0	0.0	0.0	0.01363162940059778	0.0
34-35	0.0	0.0	0.0	0.025347292456871912	0.0
36-37	0.0	0.0	0.0	0.04411433984594374	0.0
38-39	0.0	0.0	0.0	0.06946163230281566	0.0
40-41	0.0	0.0	0.0	0.09659925396667851	0.0
42-43	0.0	0.0	0.0	0.12381539884137431	0.0
44-45	0.0	0.0	0.0	0.15754897021520387	0.0
46-47	0.0	0.0	0.0	0.1995902972951581	0.0
48-49	3.140928433317461E-5	0.0	0.0	0.24846314371757777	0.0
50-51	3.140928433317461E-5	0.0	0.0	0.2966606905268342	0.0
52-53	3.140928433317461E-5	0.0	0.0	0.34432427950242667	0.0
54-55	3.140928433317461E-5	0.0	0.0	0.3979713371434889	0.0
56-57	3.140928433317461E-5	0.0	0.0	0.4650458638369833	0.0
58-59	3.140928433317461E-5	0.0	0.0	0.5409621040702663	0.0
60-61	3.140928433317461E-5	0.0	0.0	0.6241024797001795	0.0
62-63	3.140928433317461E-5	0.0	0.0	0.7082322477865878	0.0
64-65	3.140928433317461E-5	0.0	0.0	0.7977330034939688	0.0
66-67	3.140928433317461E-5	0.0	0.0	0.8993734475961218	0.0
68-69	3.140928433317461E-5	0.0	0.0	1.018210474870688	0.0
70-71	3.140928433317461E-5	0.0	0.0	1.1534745578515044	0.0
72-73	3.140928433317461E-5	0.0	0.0	1.2969364640432794	0.0
74-75	3.140928433317461E-5	0.0	0.0	1.4425184969275437	0.0
76-77	3.140928433317461E-5	0.0	0.0	1.6038051719783954	0.0
78-79	3.140928433317461E-5	0.0	0.0	1.7928576543797734	0.0
80-81	3.140928433317461E-5	0.0	0.0	2.008827893454682	0.0
82-83	3.140928433317461E-5	0.0	0.0	2.2337026646380456	0.0
84-85	3.140928433317461E-5	0.0	0.0	2.4560489884325887	0.0
86-87	3.140928433317461E-5	0.0	0.0	2.695591895399545	0.0
88	3.140928433317461E-5	0.0	0.0	2.893925821321376	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4025	0.0	46.638252	1
GTATCAA	7325	0.0	38.344406	1
ATCAACG	8265	0.0	32.67004	3
TCAACGC	8295	0.0	32.662224	4
CAACGCA	9010	0.0	30.175156	5
TATCAAC	9305	0.0	30.030153	2
GTACATG	17010	0.0	29.567099	1
TACATGG	16720	0.0	28.726114	2
AACGCAG	9675	0.0	28.271553	6
CATGGGT	8070	0.0	27.394537	4
ACATGGG	17180	0.0	26.806501	3
CGCAGAG	10545	0.0	25.358858	8
ACGCAGA	11020	0.0	24.479036	7
GCAGAGT	11760	0.0	22.059853	9
ATGGGTG	4320	0.0	21.9945	5
ATGGGTA	3600	0.0	21.820284	5
CATGGGG	6635	0.0	19.991611	4
TGGTATC	2025	0.0	18.816872	2
AGAGTAC	10920	0.0	18.419954	10-11
GAGTACA	12015	0.0	18.40388	1
>>END_MODULE