FastQCFastQC Report
Wed 25 May 2016
SRR2431404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431404_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183772
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276280.8677757075569482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221720.6964066522351475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157310.4940994518451698No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53720.16873067543781403No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50540.15874252301986447No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49950.15688937524420718No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44610.14011681741029194No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41140.12921779574668035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87500.066.6953051
GTATCAA175450.045.618161
TGGTATC26450.036.4445422
GTGGTAT28250.034.623941
ATCAACG234850.033.912963
TATCAAC236900.033.64412
TCAACGC237550.033.4879234
CAACGCA245200.032.4623035
AACGCAG253900.031.3566636
ACGCAGA284300.027.8879997
CGCAGAG285500.027.7707828
GTACATG155350.026.1234861
CATGGGT73150.026.0948354
GCAGAGT303900.025.888329
TACATGG155800.025.472952
ACATGGG155700.024.4288373
AGAGTAC296400.022.37319210-11
CAGAGTA305800.022.00052310-11
GAGTACT227050.021.8792612-13
AGTACTT230350.021.56581712-13
Produced by FastQC (version 0.11.5)